Standard name | |
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Human Ortholog | |
Description | ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 111 | 74 | 157 | 94 | 107 | 175 | 214 | 142 | – | 147 | 153 | 182 | 62 | 49 | 38 | 198 | 168 | 117 | 21 | 18 | 35 | 31 | 20 | 19 |
Bud | 12 | 11 | 20 | 11 | 7 | 12 | 24 | 15 | – | 16 | 32 | 18 | 3 | 1 | 2 | 29 | 37 | 10 | 2 | 3 | 1 | 3 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 338 | 166 | 233 | 166 | 140 | 161 | 170 | 107 | – | 153 | 125 | 192 | 142 | 151 | 151 | 465 | 257 | 278 | 468 | 217 | 333 | 203 | 293 | 341 |
Cytoplasm | 10 | 18 | 13 | 13 | 13 | 11 | 15 | 16 | – | 11 | 6 | 6 | 12 | 13 | 18 | 74 | 82 | 56 | 1 | 2 | 1 | 0 | 1 | 1 |
Endoplasmic Reticulum | 47 | 18 | 48 | 34 | 51 | 78 | 137 | 120 | – | 96 | 86 | 72 | 14 | 6 | 12 | 27 | 23 | 21 | 5 | 10 | 18 | 11 | 7 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 1 | 2 | 2 | 1 | 1 | 1 | 0 |
Golgi | 3 | 1 | 4 | 8 | 5 | 0 | 16 | 5 | – | 6 | 0 | 1 | 1 | 0 | 0 | 17 | 16 | 14 | 1 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 115 | 81 | 204 | 176 | 125 | 210 | 323 | 275 | – | 323 | 274 | 329 | 5 | 4 | 5 | 58 | 49 | 48 | 5 | 3 | 4 | 11 | 7 | 5 |
Nucleus | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 2 | – | 2 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | – | 10 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 10 | 2 | 6 | 8 | 17 | 8 | – | 10 | 3 | 10 | 1 | 0 | 0 | 12 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 2 | 1 | 1 | 0 | 19 | 2 | 2 | 1 | 3 | 1 | 0 | 1 | 0 |
Unique Cell Count | 440 | 230 | 368 | 272 | 260 | 342 | 536 | 420 | 443 | 370 | 446 | 174 | 170 | 178 | 666 | 483 | 411 | 513 | 269 | 408 | 268 | 341 | 381 | |
Labelled Cell Count | 642 | 370 | 691 | 506 | 457 | 659 | 920 | 694 | 775 | 681 | 819 | 241 | 225 | 227 | 914 | 640 | 554 | 513 | 269 | 408 | 268 | 341 | 381 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.5 | 14.8 | 12.7 | 13.0 | 13.6 | 12.4 | 12.3 | 11.8 | – | 11.2 | 10.7 | 11.3 | 13.5 | 13.3 | 13.1 | 10.2 | 11.4 | 13.5 | 14.2 | 15.0 | 15.0 |
Std Deviation (1e-4) | 2.1 | 2.9 | 2.2 | 2.1 | 2.5 | 2.2 | 2.2 | 2.1 | – | 2.2 | 2.2 | 2.2 | 2.3 | 1.9 | 2.3 | 1.9 | 2.4 | 2.9 | 2.3 | 2.3 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.1 | -0.04 | -0.05 | -0.1 | – | -0.17 | -0.24 | -0.16 | 0.09 | 0.07 | 0.05 | -0.32 | -0.16 | 0.09 | 0.17 | 0.24 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.1 | -0.4 | 2.3 | -0.8 | -2.6 | – | -2.8 | -0.4 | -0.5 | -1.6 | -3.1 | -4.9 | -4.2 | -2.3 | -4.1 |
Bud | -0.8 | -1.7 | -1.2 | -0.7 | -1.3 | – | -1.3 | 1.7 | -0.9 | -2.0 | -2.7 | -2.4 | -0.8 | 1.3 | -2.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -2.4 | -4.4 | -9.4 | -10.7 | – | -8.2 | -8.0 | -5.8 | 4.3 | 6.1 | 5.2 | 2.1 | -3.0 | 1.3 |
Cytoplasm | 0.8 | 0.9 | -0.2 | -0.6 | 0.2 | – | -0.9 | -1.6 | -2.1 | 1.7 | 2.1 | 3.1 | 4.2 | 6.2 | 4.9 |
Endoplasmic Reticulum | -0.2 | 2.2 | 3.4 | 4.6 | 5.3 | – | 3.2 | 3.6 | 1.2 | -1.7 | -3.4 | -2.2 | -5.3 | -4.3 | -3.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.7 | 0 | 0 | 1.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.1 | 2.2 |
Mitochondria | 2.4 | -1.8 | 1.6 | 1.4 | 2.9 | – | 5.2 | 5.3 | 5.5 | -11.7 | -11.8 | -11.9 | -16.5 | -14.3 | -13.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.8 | -0.3 | -0.3 | 0.4 | -0.8 | – | -0.4 | -2.0 | -0.4 | 0 | 0 | 0 | -1.0 | 0 | -2.9 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0094 | 0.0083 | 0.0315 | 0.0261 | 0.0347 | 0.004 | 0.0455 | 0.0014 | 0.0273 | 0.0905 | 0.0236 | 0.0045 | 0.034 | 0.0019 | 0.148 | 0 | 0.0026 | 0.0215 |
Bud | 0.0007 | 0.0011 | 0.0056 | 0.0032 | 0.0016 | 0.0013 | 0.0045 | 0.001 | 0.0027 | 0.0009 | 0.0013 | 0.0011 | 0.0475 | 0.0004 | 0.0047 | 0 | 0.0003 | 0.0009 |
Bud Neck | 0.0046 | 0.0126 | 0.0137 | 0.0105 | 0.0034 | 0.0321 | 0.0049 | 0.0035 | 0.0114 | 0.011 | 0.0027 | 0.0213 | 0.0019 | 0.0098 | 0.0127 | 0.0006 | 0.0191 | 0.1049 |
Bud Periphery | 0.0054 | 0.004 | 0.0711 | 0.0438 | 0.0216 | 0.0203 | 0.0252 | 0.0103 | 0.0296 | 0.0103 | 0.0132 | 0.014 | 0.0247 | 0.0022 | 0.0641 | 0.001 | 0.0048 | 0.0146 |
Bud Site | 0.0159 | 0.2212 | 0.0576 | 0.0129 | 0.0031 | 0.0038 | 0.0157 | 0.1416 | 0.0308 | 0.0058 | 0.0051 | 0.0021 | 0.0772 | 0.3957 | 0.0197 | 0.0003 | 0.0055 | 0.0048 |
Cell Periphery | 0.6252 | 0.5235 | 0.6517 | 0.5564 | 0.7534 | 0.8529 | 0.6799 | 0.7674 | 0.7909 | 0.7186 | 0.826 | 0.8945 | 0.0011 | 0.5482 | 0.6887 | 0.9552 | 0.7235 | 0.749 |
Cytoplasm | 0.0352 | 0.1387 | 0.0475 | 0.0237 | 0.0127 | 0.0159 | 0.0103 | 0.0164 | 0.0114 | 0.0071 | 0.003 | 0.0056 | 0.1662 | 0.0324 | 0.0064 | 0.0012 | 0.0087 | 0.0109 |
Cytoplasmic Foci | 0.0091 | 0.0062 | 0.0129 | 0.0704 | 0.0477 | 0.0038 | 0.0096 | 0.0001 | 0.0041 | 0.0094 | 0.0095 | 0.0013 | 0.0451 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0001 |
Eisosomes | 0.0325 | 0.0166 | 0.0374 | 0.0382 | 0.0038 | 0.0095 | 0.0909 | 0.0473 | 0.0422 | 0.0254 | 0.0662 | 0.0359 | 0.0003 | 0.0037 | 0.0394 | 0.0255 | 0.2274 | 0.0867 |
Endoplasmic Reticulum | 0.0295 | 0.0103 | 0.0137 | 0.0132 | 0.0017 | 0.0088 | 0.0219 | 0.0038 | 0.013 | 0.0102 | 0.0082 | 0.0084 | 0.0052 | 0.0006 | 0.0029 | 0.0099 | 0.0036 | 0.0015 |
Endosome | 0.0954 | 0.026 | 0.0162 | 0.0616 | 0.0162 | 0.008 | 0.0157 | 0 | 0.006 | 0.0079 | 0.0013 | 0.0002 | 0.0206 | 0 | 0.0007 | 0 | 0 | 0 |
Golgi | 0.0027 | 0.0045 | 0.0093 | 0.0227 | 0.0439 | 0.0105 | 0.0075 | 0.0001 | 0.0065 | 0.015 | 0.0023 | 0.0005 | 0.0046 | 0 | 0.003 | 0 | 0.0005 | 0.0003 |
Lipid Particles | 0.0047 | 0.005 | 0.0051 | 0.0193 | 0.0055 | 0.0008 | 0.0078 | 0.0002 | 0.0067 | 0.023 | 0.0029 | 0.0019 | 0.0021 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0001 |
Mitochondria | 0.003 | 0.0006 | 0.0038 | 0.0559 | 0.0046 | 0.016 | 0.0208 | 0.0005 | 0.0027 | 0.0274 | 0.0068 | 0.001 | 0.0401 | 0 | 0.0024 | 0.0001 | 0.0005 | 0.0003 |
None | 0.0118 | 0.0038 | 0.0007 | 0.0023 | 0.0004 | 0.0004 | 0.017 | 0.0006 | 0.001 | 0.0021 | 0.0006 | 0.0005 | 0.0285 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0002 |
Nuclear Periphery | 0.0631 | 0.0011 | 0.0017 | 0.0032 | 0.0002 | 0.0012 | 0.0049 | 0.0014 | 0.002 | 0.0009 | 0.0007 | 0.0007 | 0.0503 | 0.0003 | 0.0009 | 0.001 | 0.0007 | 0.0003 |
Nucleolus | 0.0012 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.0185 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Nucleus | 0.0105 | 0.0009 | 0.0011 | 0.0007 | 0.0001 | 0.0006 | 0.0011 | 0.0003 | 0.0004 | 0.0006 | 0.0003 | 0.0002 | 0.3164 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0003 |
Peroxisomes | 0.0014 | 0.0003 | 0.0062 | 0.0106 | 0.0437 | 0.0006 | 0.0079 | 0 | 0.0036 | 0.0278 | 0.0209 | 0.0024 | 0.0103 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0192 | 0.0002 | 0.0007 | 0.0148 | 0.0004 | 0 | 0.0009 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0826 | 0 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.0153 | 0.0132 | 0.0107 | 0.0089 | 0.0012 | 0.0077 | 0.0063 | 0.004 | 0.0067 | 0.0046 | 0.0048 | 0.0038 | 0.015 | 0.0035 | 0.0036 | 0.0046 | 0.0015 | 0.0033 |
Vacuole Periphery | 0.0042 | 0.0013 | 0.001 | 0.0012 | 0.0001 | 0.0016 | 0.0011 | 0.0001 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0076 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.9969 | 57.448 | 37.0416 | 37.514 | 44.0873 | 33.6731 | 51.3183 | 46.4356 | 35.2161 | 52.0117 |
Translational Efficiency | 0.7202 | 0.7037 | 0.7757 | 0.6787 | 0.6396 | 0.9534 | 0.6914 | 0.5746 | 0.6403 | 0.5513 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1958 | 920 | 2058 | 319 | 2062 | 1031 | 3039 | 1779 | 4020 | 1951 | 5097 | 2098 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1198.64 | 1237.60 | 1383.30 | 1302.26 | 1095.35 | 1266.01 | 1399.89 | 1269.06 | 1145.66 | 1252.61 | 1393.19 | 1274.11 |
Standard Deviation | 168.05 | 213.71 | 203.45 | 208.42 | 161.63 | 168.93 | 163.08 | 171.07 | 172.69 | 191.88 | 180.65 | 177.66 |
Intensity Change Log 2 | — | 0.046147 | 0.206716 | 0.119619 | — | 0.208897 | 0.353922 | 0.212368 | — | 0.126151 | 0.278880 | 0.164650 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.017879 | 0.011902 | 0.004269 | 0.016081 | 0.005153 | 0.005849 | 0.001784 | 0.007033 | 0.011351 | 0.008703 | 0.002788 | 0.008409 |
Bud Neck | 0.007611 | 0.005819 | 0.003490 | 0.009823 | 0.002111 | 0.002218 | 0.001318 | 0.003633 | 0.004790 | 0.003916 | 0.002195 | 0.004574 |
Bud Site | 0.033188 | 0.019287 | 0.013792 | 0.050027 | 0.006716 | 0.010431 | 0.002826 | 0.015922 | 0.019609 | 0.014607 | 0.007254 | 0.021107 |
Cell Periphery | 0.886229* | 0.813470* | 0.918774* | 0.861680* | 0.926759* | 0.945519* | 0.977325* | 0.951146* | 0.907018* | 0.883251* | 0.953684* | 0.937542* |
Cytoplasm | 0.007035 | 0.004377 | 0.008343 | 0.010484 | 0.001269 | 0.001147 | 0.000503 | 0.003410 | 0.004077 | 0.002670 | 0.003668 | 0.004486 |
Cytoplasmic Foci | 0.001745 | 0.004464 | 0.001734 | 0.002411 | 0.000994 | 0.001328 | 0.000188 | 0.000255 | 0.001360 | 0.002807 | 0.000812 | 0.000583 |
Eisosomes | 0.013326 | 0.011092 | 0.004035 | 0.007834 | 0.017220 | 0.010614 | 0.007597 | 0.005599 | 0.015324 | 0.010839 | 0.006159 | 0.005939 |
Endoplasmic Reticulum | 0.014727 | 0.100280* | 0.027202 | 0.022554 | 0.027897 | 0.010380 | 0.004329 | 0.006134 | 0.021482 | 0.052773 | 0.013565 | 0.008631 |
Endosome | 0.002987 | 0.007526 | 0.002137 | 0.002931 | 0.003111 | 0.002971 | 0.000781 | 0.001181 | 0.003051 | 0.005119 | 0.001329 | 0.001447 |
Golgi | 0.003261 | 0.003567 | 0.001380 | 0.002071 | 0.000857 | 0.001135 | 0.000262 | 0.000627 | 0.002028 | 0.002282 | 0.000714 | 0.000847 |
Lipid Particles | 0.000973 | 0.004720 | 0.005472 | 0.003636 | 0.000455 | 0.000150 | 0.000103 | 0.000344 | 0.000708 | 0.002305 | 0.002271 | 0.000845 |
Mitochondria | 0.005505 | 0.003081 | 0.000702 | 0.000474 | 0.002892 | 0.004924 | 0.000359 | 0.000962 | 0.004165 | 0.004055 | 0.000498 | 0.000888 |
Mitotic Spindle | 0.001013 | 0.000838 | 0.000742 | 0.000869 | 0.000546 | 0.000560 | 0.000337 | 0.000464 | 0.000774 | 0.000691 | 0.000501 | 0.000526 |
None | 0.000049 | 0.000292 | 0.000048 | 0.000033 | 0.000081 | 0.000035 | 0.000019 | 0.000028 | 0.000066 | 0.000156 | 0.000031 | 0.000029 |
Nuclear Periphery | 0.000311 | 0.000957 | 0.001291 | 0.000554 | 0.000687 | 0.000475 | 0.000566 | 0.000511 | 0.000504 | 0.000703 | 0.000859 | 0.000517 |
Nuclear Periphery Foci | 0.000130 | 0.000309 | 0.000287 | 0.000574 | 0.000068 | 0.000088 | 0.000039 | 0.000088 | 0.000098 | 0.000192 | 0.000139 | 0.000162 |
Nucleolus | 0.000020 | 0.000034 | 0.000038 | 0.000018 | 0.000034 | 0.000019 | 0.000018 | 0.000016 | 0.000027 | 0.000026 | 0.000026 | 0.000016 |
Nucleus | 0.000193 | 0.000176 | 0.000225 | 0.000241 | 0.000094 | 0.000066 | 0.000067 | 0.000120 | 0.000142 | 0.000118 | 0.000130 | 0.000138 |
Peroxisomes | 0.001523 | 0.005250 | 0.002023 | 0.000752 | 0.002211 | 0.000945 | 0.000494 | 0.000226 | 0.001876 | 0.002975 | 0.001111 | 0.000306 |
Vacuole | 0.001238 | 0.001240 | 0.003089 | 0.004908 | 0.000308 | 0.000587 | 0.000702 | 0.001508 | 0.000761 | 0.000895 | 0.001666 | 0.002024 |
Vacuole Periphery | 0.001055 | 0.001318 | 0.000926 | 0.002043 | 0.000537 | 0.000559 | 0.000382 | 0.000793 | 0.000789 | 0.000917 | 0.000602 | 0.000983 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.41 | 8.44 | 0.44 | -0.99 | -3.10 | -0.50 | 3.96 | -1.33 | -0.71 | -4.30 | 1.89 | 9.55 | 2.05 | 0.18 | -4.72 |
Bud Neck | 2.37 | 6.57 | -1.85 | -3.39 | -5.74 | -0.31 | 2.98 | -2.94 | -2.69 | -4.83 | 2.14 | 7.86 | 0.41 | -1.29 | -5.29 |
Bud Site | 4.32 | 6.88 | -2.35 | -4.39 | -5.30 | -1.74 | 3.30 | -4.20 | -2.08 | -6.71 | 2.60 | 8.35 | -0.64 | -2.76 | -6.87 |
Cell Periphery | 7.26 | -5.09 | 1.80 | -3.14 | 4.32 | -3.13 | -13.57 | -5.01 | -0.94 | 7.23 | 4.03 | -13.10 | -6.52 | -8.83 | 4.09 |
Cytoplasm | 2.31 | -1.05 | -1.91 | -3.43 | -1.16 | 0.26 | 2.27 | -3.16 | -3.35 | -4.91 | 2.32 | 0.71 | -0.57 | -2.61 | -1.21 |
Cytoplasmic Foci | -2.80 | 0.03 | -0.49 | 1.30 | -0.51 | -0.64 | 4.28 | 3.79 | 2.20 | -1.02 | -2.70 | 2.38 | 2.72 | 4.14 | 0.97 |
Eisosomes | 1.76 | 12.13 | 4.16 | 2.15 | -3.38 | 3.96 | 7.87 | 9.50 | 3.96 | 3.55 | 4.23 | 12.41 | 11.92 | 5.53 | 0.48 |
Endoplasmic Reticulum | -13.54 | -5.26 | -1.68 | 10.17 | 0.95 | 7.58 | 11.52 | 10.48 | 3.03 | -2.02 | -9.28 | 5.33 | 8.66 | 13.44 | 3.87 |
Endosome | -7.49 | 2.27 | 0.06 | 4.53 | -0.89 | 0.23 | 10.20 | 6.59 | 2.98 | -1.73 | -4.80 | 7.98 | 5.36 | 8.30 | -0.50 |
Golgi | -0.33 | 2.25 | 1.18 | 2.05 | -1.14 | -0.68 | 4.11 | 0.90 | 1.12 | -1.54 | -0.51 | 3.18 | 2.58 | 3.97 | -0.60 |
Lipid Particles | -3.76 | -5.58 | -2.71 | 0.82 | 1.56 | 2.97 | 3.61 | 0.71 | -1.47 | -1.88 | -3.35 | -4.55 | -0.56 | 3.04 | 4.10 |
Mitochondria | 2.10 | 4.40 | 4.59 | 6.64 | 1.78 | -1.39 | 8.68 | 5.39 | 2.72 | -2.77 | 0.12 | 6.63 | 5.64 | 3.94 | -2.11 |
Mitotic Spindle | 0.41 | 0.64 | 0.33 | -0.24 | -1.01 | -0.31 | 7.58 | 2.20 | 2.00 | -4.17 | 0.39 | 1.31 | 1.18 | 3.87 | -0.77 |
None | -1.19 | 0.14 | 2.83 | 1.27 | 2.51 | 6.33 | 10.74 | 8.78 | 1.49 | -3.73 | -0.95 | 8.27 | 9.00 | 1.33 | 0.76 |
Nuclear Periphery | -8.30 | -18.43 | -2.16 | 2.98 | 6.00 | 4.13 | 3.02 | 3.46 | -0.63 | 1.21 | -4.33 | -11.32 | -0.32 | 3.30 | 7.56 |
Nuclear Periphery Foci | -6.74 | -6.39 | -3.12 | -1.87 | -2.03 | -2.75 | 6.90 | -2.72 | 0.07 | -7.19 | -6.95 | -3.53 | -2.62 | 1.24 | -0.99 |
Nucleolus | -6.74 | -10.66 | 0.74 | 4.53 | 5.89 | 6.53 | 9.99 | 10.73 | 1.37 | 2.24 | 0.59 | 0.68 | 8.77 | 6.29 | 9.21 |
Nucleus | 0.94 | -1.63 | -1.26 | -1.84 | -0.47 | 3.37 | 2.98 | -1.10 | -2.23 | -2.17 | 2.45 | 1.19 | 0.16 | -0.97 | -0.40 |
Peroxisomes | -3.87 | -1.23 | 2.39 | 4.81 | 3.78 | 2.36 | 3.72 | 4.50 | 2.34 | 1.88 | -2.05 | 2.54 | 5.91 | 5.71 | 5.38 |
Vacuole | 0.01 | -6.43 | -3.36 | -3.39 | -1.66 | -2.40 | -2.57 | -3.22 | -2.38 | -2.02 | -1.06 | -5.76 | -3.44 | -3.10 | -0.96 |
Vacuole Periphery | -0.73 | 0.38 | -0.60 | -0.45 | -0.69 | -0.19 | 1.02 | -0.96 | -0.82 | -1.37 | -0.69 | 1.01 | -0.53 | -0.20 | -1.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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