Standard name | |
---|---|
Human Ortholog | |
Description | Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 2 | 5 | 5 |
Bud | 1 | 4 | 0 | 0 | 0 | 4 | 4 | 1 | 5 | 7 | 10 | 2 | 5 | 2 | 1 | – | 0 | 0 | 0 | 3 | 0 | 1 | 16 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 11 | 12 | 5 | 3 | 3 | 8 | 3 | 7 | 3 | 1 | 4 | 4 | 7 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 113 | 505 | 244 | 126 | 211 | 190 | 237 | 284 | 204 | 180 | 154 | 187 | 227 | 367 | 368 | – | 27 | 16 | 112 | 504 | 237 | 143 | 196 | 169 |
Endoplasmic Reticulum | 1 | 10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | – | 1 | 2 | 0 | 2 | 2 | 8 | 12 | 12 |
Endosome | 0 | 1 | 0 | 0 | 3 | 2 | 5 | 2 | 0 | 3 | 4 | 2 | 3 | 5 | 4 | – | 1 | 3 | 1 | 4 | 0 | 0 | 10 | 10 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 4 | 0 | 1 | 6 | 6 |
Mitochondria | 2 | 6 | 5 | 6 | 9 | 38 | 53 | 36 | 101 | 107 | 95 | 69 | 6 | 4 | 3 | – | 3 | 0 | 1 | 8 | 3 | 6 | 20 | 12 |
Nucleus | 0 | 2 | 1 | 1 | 2 | 0 | 3 | 9 | 2 | 7 | 3 | 7 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | – | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 5 | 5 | – | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 7 |
SpindlePole | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 3 | 0 | 4 | 4 | 4 | 1 | 3 | 3 | – | 0 | 2 | 0 | 1 | 0 | 0 | 4 | 4 |
Vac/Vac Membrane | 0 | 3 | 16 | 3 | 2 | 6 | 5 | 4 | 4 | 1 | 1 | 1 | 1 | 0 | 1 | – | 3 | 1 | 0 | 10 | 4 | 5 | 10 | 15 |
Unique Cell Count | 116 | 525 | 262 | 131 | 220 | 214 | 269 | 315 | 253 | 239 | 221 | 241 | 239 | 376 | 379 | 35 | 20 | 121 | 549 | 259 | 175 | 299 | 262 | |
Labelled Cell Count | 119 | 545 | 281 | 143 | 234 | 244 | 318 | 345 | 325 | 314 | 274 | 279 | 252 | 394 | 389 | 41 | 25 | 121 | 549 | 259 | 175 | 299 | 262 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 7.2 | 7.3 | 6.8 | 6.9 | 4.8 | 5.4 | 5.7 | 4.4 | 4.3 | 4.3 | 4.4 | 7.6 | 6.1 | 5.5 | – | 9.4 | 7.8 | 7.0 | 6.0 | 6.2 |
Std Deviation (1e-4) | 1.2 | 1.4 | 1.1 | 1.6 | 2.1 | 1.2 | 1.9 | 2.0 | 1.4 | 1.5 | 1.9 | 1.3 | 1.9 | 1.1 | 1.5 | – | 2.6 | 2.2 | 1.7 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.09 | -0.61 | -0.44 | -0.36 | -0.74 | -0.76 | -0.77 | -0.72 | 0.06 | -0.26 | -0.41 | – | 0.36 | 0.1 | -0.06 | -0.29 | -0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -0.4 | -2.0 | 0 | -1.0 | -2.7 | 0 | 0 | 0 | 0 | -1.8 | -2.0 | -3.8 | – | 0 | 0 |
Cytoplasm | 1.2 | 1.3 | -1.7 | -2.0 | -1.3 | -4.2 | -5.5 | -6.7 | -5.0 | 0.9 | 2.8 | 2.4 | – | -3.1 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 1.4 | 0 | 6.6 | 4.4 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -1.7 | -3.0 | -1.7 | -2.5 | -3.2 | -2.7 | -3.5 | -3.4 | -3.5 | -3.5 | -4.9 | -4.5 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4655 | 6.6952 | 5.8557 | 4.9203 | 4.7998 | 5.7004 | 2.6796 | 4.37 | 3.6928 | 2.9074 | 3.3296 | 3.8257 | 2.0128 | 3.1859 | 2.8728 | 3.0406 | 2.7504 | 2.9792 |
Actin | 0.0202 | 0.0004 | 0.0029 | 0.0003 | 0.0351 | 0.0064 | 0.0085 | 0.0007 | 0.0079 | 0.0107 | 0.0013 | 0.0063 | 0.0018 | 0.0001 | 0.0019 | 0.0002 | 0.0195 | 0.0016 |
Bud | 0.0013 | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0005 | 0.0028 | 0.001 | 0.0015 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.001 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0037 | 0.0003 | 0.0005 | 0.0004 | 0.0009 | 0.0012 | 0.0006 | 0.0002 | 0.0018 | 0.0067 | 0.0004 | 0.0012 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0005 |
Bud Periphery | 0.0015 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0018 | 0.0008 | 0.0012 | 0.0003 | 0.0012 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0042 | 0.0042 | 0.0014 | 0.0002 | 0.0014 | 0.0002 | 0.0015 | 0.019 | 0.0055 | 0.0035 | 0.0004 | 0.0007 | 0.0008 | 0.0006 | 0.0084 | 0.0001 | 0.0006 | 0.0001 |
Cell Periphery | 0.0012 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.8047 | 0.9022 | 0.9214 | 0.9365 | 0.8335 | 0.8957 | 0.8325 | 0.9263 | 0.9192 | 0.6702 | 0.8905 | 0.8935 | 0.8222 | 0.9317 | 0.8726 | 0.9351 | 0.8979 | 0.943 |
Cytoplasmic Foci | 0.0248 | 0.0042 | 0.0114 | 0.0052 | 0.0117 | 0.0181 | 0.0239 | 0.0073 | 0.0117 | 0.0576 | 0.0135 | 0.0191 | 0.0389 | 0.0048 | 0.0232 | 0.0077 | 0.0174 | 0.0136 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0102 | 0.002 | 0.0051 | 0.0028 | 0.0017 | 0.0063 | 0.0088 | 0.0011 | 0.007 | 0.0075 | 0.0044 | 0.0044 | 0.0056 | 0.0014 | 0.0029 | 0.004 | 0.0026 | 0.0034 |
Endosome | 0.0235 | 0.0025 | 0.0112 | 0.0052 | 0.0146 | 0.0127 | 0.0329 | 0.0133 | 0.0147 | 0.089 | 0.0369 | 0.031 | 0.0245 | 0.0024 | 0.0226 | 0.0088 | 0.0208 | 0.0129 |
Golgi | 0.0053 | 0.0003 | 0.0015 | 0.0003 | 0.0049 | 0.0055 | 0.0069 | 0.0009 | 0.003 | 0.0349 | 0.0062 | 0.0098 | 0.0042 | 0.0002 | 0.005 | 0.0003 | 0.0091 | 0.0014 |
Lipid Particles | 0.005 | 0.0002 | 0.0028 | 0.0013 | 0.0241 | 0.0018 | 0.0084 | 0.0001 | 0.0004 | 0.0067 | 0.0058 | 0.0037 | 0.0056 | 0 | 0.004 | 0.0003 | 0.0022 | 0.0008 |
Mitochondria | 0.0035 | 0.0172 | 0.0041 | 0.0003 | 0.0135 | 0.0036 | 0.0103 | 0.0002 | 0.0015 | 0.0435 | 0.0187 | 0.0152 | 0.0072 | 0.0001 | 0.0022 | 0.0003 | 0.0018 | 0.0005 |
None | 0.0562 | 0.0632 | 0.0288 | 0.0414 | 0.0187 | 0.0383 | 0.0462 | 0.0224 | 0.0176 | 0.0058 | 0.0131 | 0.0061 | 0.0729 | 0.0548 | 0.0445 | 0.0352 | 0.0204 | 0.0184 |
Nuclear Periphery | 0.0015 | 0.0003 | 0.0008 | 0.0006 | 0.0007 | 0.0012 | 0.0041 | 0.0002 | 0.0011 | 0.002 | 0.001 | 0.0007 | 0.0013 | 0.0005 | 0.001 | 0.0024 | 0.0006 | 0.0008 |
Nucleolus | 0.0041 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0.0001 | 0 | 0 |
Nucleus | 0.0055 | 0.0006 | 0.001 | 0.001 | 0.0021 | 0.0009 | 0.0019 | 0.0007 | 0.0015 | 0.0029 | 0.0012 | 0.0009 | 0.001 | 0.0013 | 0.0016 | 0.0016 | 0.001 | 0.0008 |
Peroxisomes | 0.011 | 0 | 0.0015 | 0.0001 | 0.0274 | 0.0016 | 0.0044 | 0.0001 | 0.0009 | 0.0123 | 0.0008 | 0.0017 | 0.008 | 0 | 0.002 | 0.0001 | 0.0022 | 0.0003 |
Punctate Nuclear | 0.001 | 0.0001 | 0.0005 | 0.0002 | 0.0019 | 0.0037 | 0.0016 | 0.0001 | 0.0004 | 0.0009 | 0.0002 | 0.0005 | 0.0023 | 0.0002 | 0.0008 | 0.0007 | 0.0004 | 0.0006 |
Vacuole | 0.0101 | 0.0011 | 0.0034 | 0.0026 | 0.0052 | 0.0012 | 0.0048 | 0.0022 | 0.0031 | 0.0379 | 0.0034 | 0.002 | 0.0023 | 0.0011 | 0.0032 | 0.0021 | 0.002 | 0.0009 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.0004 | 0.0005 | 0.0011 | 0.0004 | 0.0013 | 0.0003 | 0.0006 | 0.0036 | 0.0012 | 0.0009 | 0.0005 | 0.0001 | 0.0006 | 0.0005 | 0.0007 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 122.3792 | 102.8712 | 99.8225 | 133.3479 | 109.8926 | 71.6617 | 109.4136 | 109.7577 | 97.038 | 142.4559 |
Translational Efficiency | 0.6101 | 0.7221 | 0.7697 | 0.511 | 0.6185 | 1.0503 | 0.6837 | 0.5728 | 0.7319 | 0.5735 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
210 | 1580 | 237 | 1659 | 1442 | 1696 | 2237 | 1063 | 1652 | 3276 | 2474 | 2722 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 834.56 | 950.12 | 1274.20 | 1442.10 | 760.45 | 898.30 | 1274.06 | 1374.50 | 769.87 | 923.29 | 1274.07 | 1415.70 |
Standard Deviation | 117.32 | 141.82 | 195.50 | 230.56 | 131.26 | 132.94 | 185.47 | 257.71 | 131.90 | 139.71 | 186.45 | 243.77 |
Intensity Change Log 2 | — | 0.187094 | 0.610504 | 0.789084 | — | 0.240344 | 0.744508 | 0.853982 | — | 0.212727 | 0.675947 | 0.820389 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000432 | 0.000282 | 0.000632 | 0.000986 | 0.000210 | 0.000239 | 0.000134 | 0.003467 | 0.000238 | 0.000260 | 0.000181 | 0.001955 |
Bud Neck | 0.012805 | 0.006022 | 0.005169 | 0.006588 | 0.006521 | 0.004784 | 0.002530 | 0.009769 | 0.007320 | 0.005381 | 0.002783 | 0.007830 |
Bud Site | 0.039090 | 0.018115 | 0.019766 | 0.048353 | 0.013078 | 0.022982 | 0.008744 | 0.056288 | 0.016385 | 0.020634 | 0.009800 | 0.051452 |
Cell Periphery | 0.000229 | 0.000072 | 0.000199 | 0.000219 | 0.000117 | 0.000085 | 0.000057 | 0.000211 | 0.000131 | 0.000079 | 0.000070 | 0.000216 |
Cytoplasm | 0.565659* | 0.668892* | 0.428636* | 0.558880* | 0.631954* | 0.696394* | 0.783637* | 0.496334* | 0.623527* | 0.683130* | 0.749629* | 0.534454* |
Cytoplasmic Foci | 0.234801* | 0.170308 | 0.044445 | 0.105370 | 0.185169* | 0.165202 | 0.026174 | 0.121864 | 0.191479* | 0.167665 | 0.027925 | 0.111811 |
Eisosomes | 0.000123 | 0.000027 | 0.000062 | 0.000012 | 0.000057 | 0.000029 | 0.000008 | 0.000019 | 0.000066 | 0.000028 | 0.000013 | 0.000015 |
Endoplasmic Reticulum | 0.000707 | 0.001156 | 0.002206 | 0.000213 | 0.002353 | 0.000619 | 0.000439 | 0.000514 | 0.002143 | 0.000878 | 0.000608 | 0.000330 |
Endosome | 0.022959 | 0.013942 | 0.014199 | 0.004960 | 0.014484 | 0.013451 | 0.003502 | 0.010202 | 0.015561 | 0.013688 | 0.004527 | 0.007007 |
Golgi | 0.008064 | 0.004197 | 0.000507 | 0.004717 | 0.004351 | 0.008181 | 0.001145 | 0.006005 | 0.004823 | 0.006260 | 0.001084 | 0.005220 |
Lipid Particles | 0.002530 | 0.001202 | 0.004077 | 0.003621 | 0.002836 | 0.001158 | 0.000687 | 0.007142 | 0.002797 | 0.001179 | 0.001011 | 0.004996 |
Mitochondria | 0.007989 | 0.001456 | 0.000153 | 0.000285 | 0.002321 | 0.003333 | 0.000510 | 0.003191 | 0.003042 | 0.002428 | 0.000476 | 0.001420 |
Mitotic Spindle | 0.006415 | 0.005396 | 0.036495 | 0.203308* | 0.000467 | 0.003474 | 0.035926 | 0.198502* | 0.001223 | 0.004401 | 0.035981 | 0.201431* |
None | 0.000271 | 0.001106 | 0.002758 | 0.001597 | 0.004051 | 0.001039 | 0.002513 | 0.001265 | 0.003570 | 0.001071 | 0.002536 | 0.001468 |
Nuclear Periphery | 0.000047 | 0.000103 | 0.002556 | 0.000200 | 0.000202 | 0.000043 | 0.000236 | 0.000337 | 0.000182 | 0.000072 | 0.000458 | 0.000253 |
Nuclear Periphery Foci | 0.000403 | 0.000523 | 0.010505 | 0.004792 | 0.000795 | 0.000326 | 0.001963 | 0.006461 | 0.000745 | 0.000421 | 0.002782 | 0.005444 |
Nucleolus | 0.000263 | 0.000212 | 0.000506 | 0.000386 | 0.000766 | 0.000260 | 0.000602 | 0.000966 | 0.000702 | 0.000237 | 0.000593 | 0.000613 |
Nucleus | 0.009291 | 0.020729 | 0.069162 | 0.018304 | 0.049818 | 0.014079 | 0.047190 | 0.019509 | 0.044666 | 0.017286 | 0.049295 | 0.018775 |
Peroxisomes | 0.004450 | 0.002288 | 0.002647 | 0.009346 | 0.001645 | 0.002456 | 0.001518 | 0.014384 | 0.002002 | 0.002375 | 0.001626 | 0.011313 |
Vacuole | 0.080758 | 0.081155 | 0.354238* | 0.026857 | 0.075595 | 0.058870 | 0.081729 | 0.042168 | 0.076252 | 0.069618 | 0.107834 | 0.032836 |
Vacuole Periphery | 0.002712 | 0.002817 | 0.001081 | 0.001005 | 0.003210 | 0.002997 | 0.000755 | 0.001401 | 0.003147 | 0.002910 | 0.000786 | 0.001160 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.49 | -2.22 | -1.21 | -2.49 | 0.53 | -0.58 | 0.79 | -1.86 | -1.82 | -1.91 | -0.47 | 0.26 | -2.52 | -2.46 | -2.57 |
Bud Neck | 3.11 | 3.13 | 3.18 | -0.06 | -0.21 | 2.49 | 5.56 | -0.87 | -2.87 | -5.14 | 2.73 | 6.50 | 0.98 | -1.99 | -6.52 |
Bud Site | 4.59 | 4.89 | 0.01 | -8.40 | -7.91 | -6.01 | 3.44 | -10.45 | -7.06 | -12.38 | -2.93 | 5.47 | -12.10 | -10.69 | -17.31 |
Cell Periphery | 6.54 | 0.88 | 1.12 | -7.09 | 0.31 | 2.20 | 7.74 | -5.18 | -5.81 | -8.38 | 5.61 | 7.66 | -5.17 | -8.65 | -9.69 |
Cytoplasm | -5.21 | 8.54 | 4.11 | 16.21 | -6.65 | -7.06 | -7.84 | 14.20 | 19.43 | 20.10 | -7.57 | -5.11 | 16.29 | 25.23 | 21.44 |
Cytoplasmic Foci | 4.81 | 15.67 | 8.22 | 7.03 | -15.77 | 3.09 | 32.20 | 6.91 | 4.57 | -15.11 | 4.44 | 35.54 | 11.18 | 8.38 | -22.86 |
Eisosomes | 5.09 | 3.58 | 6.00 | 8.52 | 9.38 | 6.96 | 13.41 | 11.23 | 5.73 | -2.59 | 9.10 | 13.02 | 13.33 | 9.99 | 1.79 |
Endoplasmic Reticulum | -1.55 | -6.16 | 2.75 | 4.71 | 10.04 | 2.82 | 3.21 | 3.39 | 2.29 | 1.40 | 2.34 | 2.87 | 3.66 | 5.40 | 7.81 |
Endosome | 2.69 | 3.83 | 5.96 | 10.18 | 6.27 | 0.89 | 12.56 | 7.60 | 6.30 | -4.16 | 1.82 | 13.09 | 11.94 | 12.13 | -2.10 |
Golgi | 1.74 | 3.46 | 2.62 | 3.29 | -3.47 | -5.41 | 9.10 | 2.35 | 6.18 | -2.69 | -2.55 | 9.18 | 4.37 | 7.28 | -3.74 |
Lipid Particles | 2.03 | -1.53 | -3.71 | -8.88 | -2.93 | 5.19 | 6.45 | -6.03 | -7.81 | -7.91 | 6.48 | 5.62 | -7.61 | -11.50 | -11.05 |
Mitochondria | 1.25 | 1.48 | 1.49 | 2.84 | 1.56 | -1.09 | 4.03 | 1.11 | 1.79 | -0.90 | 0.69 | 3.41 | 2.85 | 3.16 | -0.66 |
Mitotic Spindle | 0.23 | -2.90 | -24.49 | -27.54* | -18.27 | -2.93 | -14.03 | -21.63 | -21.21 | -15.32 | -3.16 | -14.18 | -35.47 | -34.83* | -24.40 |
None | -2.88 | -5.57 | -10.68 | -1.90 | 1.57 | 3.86 | 2.28 | 3.56 | -1.35 | 4.10 | 3.65 | 1.91 | 3.04 | -2.59 | 3.57 |
Nuclear Periphery | -3.74 | -4.05 | -17.06 | -12.59 | 3.40 | 6.58 | -8.35 | -7.68 | -15.78 | 1.17 | 4.95 | -7.89 | -9.89 | -20.57 | 4.02 |
Nuclear Periphery Foci | -1.83 | -6.21 | -11.55 | -11.33 | 1.90 | 4.68 | -7.06 | -11.13 | -11.89 | -7.68 | 3.77 | -9.18 | -15.37 | -16.31 | -7.65 |
Nucleolus | 0.39 | -1.24 | -1.49 | -4.04 | 0.58 | 3.94 | 1.25 | -0.19 | -1.77 | -0.84 | 4.69 | 0.90 | 0.42 | -2.94 | -0.44 |
Nucleus | -7.16 | -9.43 | -7.26 | -0.18 | 8.15 | 12.96 | -2.94 | 9.40 | -4.83 | 17.18 | 11.36 | -6.18 | 9.44 | -3.93 | 21.37 |
Peroxisomes | 2.48 | 2.93 | -0.28 | -3.14 | -3.42 | -2.66 | 1.98 | -4.91 | -4.07 | -5.43 | -1.42 | 2.98 | -5.49 | -4.98 | -6.71 |
Vacuole | -0.46 | -16.81 | 2.65 | 6.08 | 18.81 | 4.70 | -11.07 | -2.42 | -5.80 | 6.69 | 1.73 | -15.98 | 1.52 | -0.03 | 17.25 |
Vacuole Periphery | -0.09 | 3.21 | 3.35 | 5.27 | 0.44 | 0.33 | 5.09 | 4.74 | 6.25 | -0.71 | 0.47 | 5.58 | 5.40 | 8.04 | -0.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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