Standard name | |
---|---|
Human Ortholog | |
Description | Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 1 | 3 | 2 | 7 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 1 | 2 | 3 |
Bud | 5 | 6 | 2 | 0 | 3 | 5 | 9 | 15 | 9 | 23 | 11 | 14 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 8 | 6 | 0 | 10 | 15 |
Bud Neck | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 28 | 36 | 95 | 50 | 43 | 72 | 70 | 94 | 94 | 108 | 58 | 81 | 52 | 46 | 57 | 93 | 67 | 91 | 19 | 6 | 58 | 10 | 18 | 26 |
Cytoplasm | 177 | 183 | 263 | 135 | 169 | 229 | 300 | 330 | 232 | 327 | 167 | 172 | 282 | 227 | 341 | 406 | 318 | 311 | 150 | 155 | 169 | 134 | 249 | 211 |
Endoplasmic Reticulum | 0 | 0 | 3 | 4 | 1 | 4 | 1 | 6 | 3 | 2 | 3 | 4 | 1 | 0 | 3 | 11 | 4 | 6 | 0 | 0 | 6 | 1 | 0 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 24 | 1 | 2 | 5 | 14 | 52 | 93 | 122 | 95 | 131 | 87 | 85 | 0 | 0 | 0 | 3 | 3 | 1 | 7 | 1 | 15 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 3 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 6 | 4 | 3 | 0 | 0 | 0 | 0 | 3 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 1 | 4 | 0 | 2 | 1 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 0 | 1 | 4 | 8 | 4 | 9 | 2 | 1 | 0 | 2 | 1 | 20 | 7 | 5 | 2 | 1 | 1 | 0 | 1 | 0 |
Unique Cell Count | 192 | 185 | 278 | 146 | 192 | 273 | 361 | 435 | 330 | 447 | 242 | 260 | 283 | 231 | 345 | 433 | 327 | 322 | 197 | 183 | 277 | 157 | 299 | 272 |
Labelled Cell Count | 235 | 227 | 370 | 196 | 235 | 367 | 484 | 590 | 449 | 607 | 334 | 360 | 336 | 276 | 404 | 541 | 404 | 419 | 197 | 183 | 277 | 157 | 299 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 6.5 | 6.5 | 6.1 | 5.6 | 5.1 | 5.2 | 5.3 | 5.3 | 5.3 | 5.0 | 5.5 | 8.1 | 7.8 | 7.4 | 9.7 | 9.0 | 9.2 | 7.7 | 7.8 | 8.5 |
Std Deviation (1e-4) | 1.8 | 2.4 | 2.2 | 2.5 | 2.2 | 2.1 | 2.2 | 1.7 | 1.7 | 1.6 | 1.8 | 1.6 | 2.1 | 2.1 | 2.2 | 1.4 | 1.5 | 1.4 | 2.1 | 2.1 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.22 | -0.34 | -0.32 | -0.28 | -0.29 | -0.28 | -0.36 | -0.23 | 0.33 | 0.26 | 0.2 | 0.58 | 0.47 | 0.5 | 0.25 | 0.28 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2.3 | 1.9 | 3.2 | 2.8 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -2.8 | -2.0 | -4.2 | -3.7 | -1.5 | -2.9 | -2.5 | -0.7 | -4.3 | -3.6 | -5.1 | -3.7 | -3.8 | -1.6 |
Cytoplasm | -0.9 | -2.6 | -4.1 | -4.5 | -6.5 | -7.7 | -7.2 | -7.7 | -8.4 | 3.6 | 2.2 | 3.1 | -0.5 | 1.7 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.9 | 7.2 | 8.8 | 9.4 | 9.4 | 9.7 | 10.6 | 10.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0931 | 2.1751 | 2.3224 | 2.4252 | 2.2483 | 1.9016 | 1.7283 | 1.3037 | 1.6359 | 0.5085 | 1.724 | 1.6753 | 0.8321 | 0.8889 | 0.73 | 1.3223 | 1.8333 | 0.7933 |
Actin | 0.0165 | 0.0021 | 0.0023 | 0.0443 | 0.0015 | 0.0058 | 0.0261 | 0.0027 | 0.0088 | 0.002 | 0.0058 | 0.0117 | 0.012 | 0.0018 | 0.0084 | 0.0043 | 0.0772 | 0.0141 |
Bud | 0.0011 | 0.0012 | 0.0008 | 0.0156 | 0.0062 | 0.0008 | 0.004 | 0.006 | 0.0027 | 0.0012 | 0.0044 | 0.0011 | 0.0011 | 0.0022 | 0.0033 | 0.0005 | 0.0034 | 0.0018 |
Bud Neck | 0.0006 | 0.0005 | 0.0011 | 0.0011 | 0.0009 | 0.0028 | 0.0123 | 0.0019 | 0.0022 | 0.0008 | 0.0027 | 0.0071 | 0.0086 | 0.0008 | 0.0056 | 0.0007 | 0.0086 | 0.0079 |
Bud Periphery | 0.0013 | 0.0047 | 0.0015 | 0.0107 | 0.0396 | 0.0008 | 0.009 | 0.0067 | 0.0085 | 0.0023 | 0.0237 | 0.0028 | 0.0021 | 0.0057 | 0.0075 | 0.0008 | 0.0059 | 0.0046 |
Bud Site | 0.0094 | 0.0063 | 0.0054 | 0.0043 | 0.0005 | 0.0002 | 0.0193 | 0.0271 | 0.0094 | 0.0022 | 0.003 | 0.0013 | 0.0108 | 0.0442 | 0.014 | 0.0003 | 0.0209 | 0.0009 |
Cell Periphery | 0.0122 | 0.0039 | 0.0107 | 0.0033 | 0.0048 | 0.0022 | 0.0032 | 0.0016 | 0.0021 | 0.0007 | 0.0024 | 0.0006 | 0.0033 | 0.0034 | 0.0057 | 0.0006 | 0.0014 | 0.0013 |
Cytoplasm | 0.4779 | 0.5183 | 0.5223 | 0.5924 | 0.6952 | 0.5997 | 0.4415 | 0.453 | 0.5025 | 0.322 | 0.4945 | 0.5505 | 0.4929 | 0.445 | 0.5035 | 0.4214 | 0.547 | 0.5062 |
Cytoplasmic Foci | 0.0168 | 0.0028 | 0.0029 | 0.0047 | 0.0019 | 0.0028 | 0.013 | 0.0042 | 0.0081 | 0.0229 | 0.0041 | 0.0107 | 0.012 | 0.0038 | 0.0051 | 0.0151 | 0.0061 | 0.005 |
Eisosomes | 0.0007 | 0.0001 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 |
Endoplasmic Reticulum | 0.0033 | 0.0022 | 0.006 | 0.0038 | 0.0039 | 0.0015 | 0.0048 | 0.0011 | 0.0029 | 0.0051 | 0.0016 | 0.0018 | 0.0026 | 0.0018 | 0.0019 | 0.0011 | 0.0026 | 0.0013 |
Endosome | 0.0093 | 0.0005 | 0.0021 | 0.001 | 0.0006 | 0.0012 | 0.0153 | 0.0004 | 0.007 | 0.0105 | 0.0083 | 0.0046 | 0.0042 | 0.0006 | 0.0019 | 0.0085 | 0.0125 | 0.0016 |
Golgi | 0.0031 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0048 | 0.0002 | 0.001 | 0.0014 | 0.0033 | 0.0014 | 0.0015 | 0.0002 | 0.0011 | 0.0146 | 0.0036 | 0.0007 |
Lipid Particles | 0.0098 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0159 | 0.0001 | 0.001 | 0.0007 | 0.0029 | 0.0006 | 0.0027 | 0.0002 | 0.0022 | 0.0078 | 0.0052 | 0.0022 |
Mitochondria | 0.0011 | 0.0002 | 0.0002 | 0.0011 | 0.0008 | 0.0001 | 0.0061 | 0.0003 | 0.0025 | 0.0025 | 0.0025 | 0.001 | 0.0005 | 0.0002 | 0.0007 | 0.0008 | 0.035 | 0.0017 |
None | 0.4059 | 0.454 | 0.44 | 0.307 | 0.2407 | 0.3804 | 0.3773 | 0.483 | 0.4304 | 0.595 | 0.4297 | 0.3981 | 0.4349 | 0.4865 | 0.4265 | 0.5149 | 0.2508 | 0.4463 |
Nuclear Periphery | 0.0108 | 0.0002 | 0.001 | 0.0008 | 0.0003 | 0.0002 | 0.0121 | 0.0004 | 0.0017 | 0.0026 | 0.0019 | 0.0011 | 0.0017 | 0.0003 | 0.0006 | 0.0003 | 0.0013 | 0.0003 |
Nucleolus | 0.0004 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0 | 0.0049 | 0.0007 | 0.0007 | 0.0041 | 0.001 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0004 |
Nucleus | 0.0052 | 0.0007 | 0.0008 | 0.0026 | 0.0004 | 0.0005 | 0.0091 | 0.003 | 0.0027 | 0.0143 | 0.0018 | 0.0016 | 0.0012 | 0.001 | 0.0015 | 0.0004 | 0.0027 | 0.0009 |
Peroxisomes | 0.0099 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0078 | 0.0002 | 0.001 | 0.001 | 0.0013 | 0.0009 | 0.0017 | 0.0001 | 0.0077 | 0.0071 | 0.002 | 0.0013 |
Punctate Nuclear | 0.0031 | 0.0002 | 0.0004 | 0.0019 | 0.0001 | 0.0002 | 0.006 | 0.0063 | 0.0026 | 0.0059 | 0.0029 | 0.002 | 0.0033 | 0.0009 | 0.0013 | 0.0004 | 0.0028 | 0.0007 |
Vacuole | 0.0012 | 0.001 | 0.0007 | 0.0014 | 0.0014 | 0.0004 | 0.0058 | 0.0008 | 0.0015 | 0.0019 | 0.0017 | 0.0006 | 0.001 | 0.0008 | 0.001 | 0.0004 | 0.0081 | 0.0006 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0014 | 0.0001 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.4564 | 30.484 | 28.0063 | 37.931 | 24.7383 | 25.1557 | 37.7941 | 42.5967 | 28.8281 | 32.2449 |
Translational Efficiency | 1.6125 | 1.1694 | 1.341 | 0.8818 | 1.2069 | 1.6936 | 1.0472 | 1.2373 | 1.9569 | 1.4928 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1174 | 491 | 2219 | 1495 | 1845 | 1571 | 619 | 128 | 3019 | 2062 | 2838 | 1623 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 743.78 | 846.40 | 989.80 | 1038.55 | 770.73 | 852.30 | 1023.26 | 1377.48 | 760.25 | 850.90 | 997.10 | 1065.28 |
Standard Deviation | 103.15 | 177.82 | 175.52 | 176.72 | 143.47 | 162.15 | 176.84 | 210.02 | 129.96 | 166.03 | 176.35 | 201.47 |
Intensity Change Log 2 | — | 0.186464 | 0.412261 | 0.481623 | — | 0.145136 | 0.408875 | 0.837734 | — | 0.165580 | 0.410539 | 0.673787 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004464 | 0.007373 | 0.001993 | 0.002710 | 0.003312 | 0.004661 | 0.000765 | 0.000168 | 0.003760 | 0.005307 | 0.001725 | 0.002510 |
Bud Neck | 0.019428 | 0.025082 | 0.010276 | 0.005891 | 0.030422 | 0.030801 | 0.003516 | 0.012259 | 0.026147 | 0.029439 | 0.008801 | 0.006393 |
Bud Site | 0.081082 | 0.096697 | 0.035625 | 0.034076 | 0.053814 | 0.070298 | 0.016344 | 0.008852 | 0.064418 | 0.076584 | 0.031420 | 0.032087 |
Cell Periphery | 0.011116 | 0.007506 | 0.001728 | 0.001674 | 0.009394 | 0.005897 | 0.000770 | 0.000317 | 0.010063 | 0.006280 | 0.001519 | 0.001567 |
Cytoplasm | 0.623034* | 0.524591* | 0.572717* | 0.539734* | 0.634843* | 0.601566* | 0.516205* | 0.688798* | 0.630251* | 0.583237* | 0.560391* | 0.551490* |
Cytoplasmic Foci | 0.102027 | 0.161704 | 0.026448 | 0.051862 | 0.091584 | 0.078620 | 0.072968 | 0.002176 | 0.095645 | 0.098404 | 0.036594 | 0.047944 |
Eisosomes | 0.004252 | 0.002371 | 0.000467 | 0.000296 | 0.005156 | 0.004114 | 0.000202 | 0.000066 | 0.004804 | 0.003699 | 0.000409 | 0.000277 |
Endoplasmic Reticulum | 0.005857 | 0.002716 | 0.003567 | 0.006973 | 0.001889 | 0.001941 | 0.002617 | 0.000116 | 0.003432 | 0.002125 | 0.003360 | 0.006432 |
Endosome | 0.003719 | 0.014419 | 0.001249 | 0.002835 | 0.004668 | 0.007961 | 0.006348 | 0.000087 | 0.004299 | 0.009498 | 0.002361 | 0.002618 |
Golgi | 0.003996 | 0.005343 | 0.000158 | 0.000238 | 0.004163 | 0.004566 | 0.000179 | 0.000045 | 0.004098 | 0.004751 | 0.000162 | 0.000223 |
Lipid Particles | 0.003926 | 0.005055 | 0.001585 | 0.002901 | 0.005141 | 0.003487 | 0.002548 | 0.000110 | 0.004668 | 0.003860 | 0.001795 | 0.002681 |
Mitochondria | 0.001633 | 0.003128 | 0.000433 | 0.000259 | 0.003079 | 0.003601 | 0.000389 | 0.000510 | 0.002517 | 0.003488 | 0.000423 | 0.000278 |
Mitotic Spindle | 0.000146 | 0.002445 | 0.003655 | 0.002183 | 0.000514 | 0.006332 | 0.001906 | 0.000059 | 0.000371 | 0.005406 | 0.003274 | 0.002016 |
None | 0.005081 | 0.002077 | 0.002714 | 0.001612 | 0.004932 | 0.001903 | 0.004187 | 0.007577 | 0.004990 | 0.001945 | 0.003036 | 0.002082 |
Nuclear Periphery | 0.000121 | 0.000156 | 0.000512 | 0.000493 | 0.000107 | 0.000134 | 0.000463 | 0.000075 | 0.000113 | 0.000139 | 0.000502 | 0.000460 |
Nuclear Periphery Foci | 0.001049 | 0.001909 | 0.002134 | 0.006173 | 0.000840 | 0.001014 | 0.005320 | 0.000020 | 0.000921 | 0.001227 | 0.002829 | 0.005688 |
Nucleolus | 0.000257 | 0.002226 | 0.000153 | 0.000132 | 0.000360 | 0.000610 | 0.000390 | 0.000099 | 0.000320 | 0.000995 | 0.000205 | 0.000130 |
Nucleus | 0.078285 | 0.047550 | 0.241719* | 0.169741* | 0.091517 | 0.074820 | 0.166833* | 0.245644* | 0.086371 | 0.068326 | 0.225386* | 0.175727* |
Peroxisomes | 0.002383 | 0.009925 | 0.000668 | 0.000396 | 0.002857 | 0.005551 | 0.000449 | 0.000078 | 0.002672 | 0.006592 | 0.000620 | 0.000371 |
Vacuole | 0.047601 | 0.076724 | 0.092116 | 0.169715 | 0.050433 | 0.091241 | 0.197408 | 0.032903 | 0.049331 | 0.087784 | 0.115082 | 0.158925 |
Vacuole Periphery | 0.000544 | 0.001003 | 0.000082 | 0.000106 | 0.000978 | 0.000884 | 0.000195 | 0.000040 | 0.000809 | 0.000912 | 0.000107 | 0.000101 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.68 | 6.03 | 4.84 | 4.64 | -1.96 | -2.82 | 7.33 | 10.76 | 11.97 | 4.00 | -3.49 | 6.98 | 4.81 | 7.00 | -2.53 |
Bud Neck | -1.93 | 7.15 | 11.94 | 7.39 | 5.65 | -0.11 | 17.54 | 4.06 | 4.09 | -1.93 | -1.86 | 15.13 | 17.93 | 16.19 | 3.47 |
Bud Site | -1.82 | 10.52 | 11.02 | 9.48 | 0.34 | -3.94 | 12.93 | 14.29 | 15.17 | 3.42 | -3.09 | 13.07 | 12.97 | 13.55 | -0.15 |
Cell Periphery | 2.23 | 8.58 | 8.68 | 4.64 | 0.72 | 4.02 | 11.44 | 11.97 | 12.00 | 2.90 | 4.93 | 13.63 | 13.55 | 10.19 | 0.16 |
Cytoplasm | 7.81 | 11.19 | 14.73 | 2.62 | 4.77 | 4.30 | 13.33 | -1.91 | -3.43 | -8.58 | 7.21 | 17.69 | 16.51 | 9.21 | 1.86 |
Cytoplasmic Foci | -7.01 | 21.54 | 14.20 | 13.97 | -10.33 | 3.22 | 5.97 | 31.31 | 25.47 | 19.47 | -0.79 | 24.85 | 18.68 | 15.49 | -4.68 |
Eisosomes | 3.54 | 12.08 | 12.70 | 4.61 | 2.51 | 1.91 | 12.72 | 13.03 | 9.87 | 4.21 | 2.70 | 16.39 | 16.98 | 10.41 | 2.41 |
Endoplasmic Reticulum | 2.82 | 1.72 | -1.10 | -6.30 | -5.61 | -0.14 | -3.81 | 9.26 | 6.86 | 10.46 | 2.81 | -1.05 | -5.22 | -8.96 | -5.46 |
Endosome | -6.02 | 8.54 | 3.29 | 6.65 | -8.40 | -4.04 | -1.81 | 10.68 | 11.07 | 12.62 | -7.04 | 6.50 | 5.67 | 9.98 | -1.15 |
Golgi | -1.46 | 8.61 | 8.48 | 6.90 | -3.50 | -0.62 | 20.97 | 21.68 | 7.98 | 11.14 | -1.19 | 18.87 | 18.66 | 9.75 | -2.72 |
Lipid Particles | -1.66 | 6.84 | 2.51 | 3.13 | -7.49 | 2.24 | 3.60 | 7.54 | 9.36 | 13.06 | 1.60 | 6.23 | 4.08 | 2.91 | -5.40 |
Mitochondria | -1.70 | 3.05 | 3.58 | 3.65 | 2.69 | -0.52 | 4.02 | 3.87 | 4.60 | -0.95 | -1.36 | 4.80 | 5.17 | 5.90 | 2.10 |
Mitotic Spindle | -1.95 | -4.73 | -3.14 | -0.23 | 0.76 | -4.02 | -1.87 | 2.51 | 4.38 | 2.23 | -4.44 | -4.77 | -2.80 | 2.04 | 0.94 |
None | 2.26 | 2.30 | 3.25 | 0.99 | 4.93 | 5.23 | 1.54 | -1.07 | -2.70 | -1.76 | 5.13 | 3.90 | 5.39 | 0.36 | 2.83 |
Nuclear Periphery | -1.32 | -15.13 | -11.08 | -9.62 | 0.71 | -1.31 | -12.48 | 1.38 | 1.99 | 12.49 | -1.57 | -21.63 | -12.53 | -11.11 | 1.52 |
Nuclear Periphery Foci | -3.62 | -8.70 | -15.19 | -10.49 | -11.02 | -2.18 | -11.52 | 16.95 | 13.90 | 13.53 | -3.66 | -14.90 | -15.41 | -14.06 | -8.02 |
Nucleolus | -2.07 | 2.71 | 3.99 | 2.22 | 1.32 | -1.53 | 0.42 | 7.32 | 3.09 | 6.21 | -2.62 | 4.06 | 7.29 | 3.39 | 3.25 |
Nucleus | 5.20 | -28.03 | -14.25 | -17.12 | 11.28 | 4.00 | -7.71 | -7.40 | -8.17 | -3.65 | 5.29 | -28.86 | -15.60 | -18.39 | 8.45 |
Peroxisomes | -5.26 | 8.39 | 13.10 | 6.68 | 1.18 | -2.98 | 9.18 | 10.75 | 6.36 | 8.32 | -5.18 | 10.23 | 13.56 | 8.45 | 1.50 |
Vacuole | -6.09 | -18.98 | -29.02 | -19.39 | -14.77 | -11.77 | -20.54 | 1.60 | 7.95 | 18.72 | -13.52 | -26.88 | -28.65 | -20.12 | -9.27 |
Vacuole Periphery | -1.89 | 2.82 | 2.73 | 4.96 | -1.06 | 0.29 | 2.75 | 3.22 | 4.68 | 4.70 | -0.42 | 3.72 | 3.78 | 5.68 | 0.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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