Standard name
Human Ortholog
Description Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0.13 0.1 0.08 0.08 0 0 0.05 0 0.16 0.16 0.1 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0.07 0.13 0 0.08 0 0 0 0 0 0 0.19 0.19 0.15 0.18 0.1 0.09 0.08 0.09 0.06 0.06 0.08
Golgi 0 0.1 0.07 0 0.08 0 0 0 0 0 0 0.15 0.07 0.13 0.2 0.23 0 0 0 0.07 0 0.06
Mitochondria 0.87 0.67 0.47 0.77 0.64 0.78 0.78 0.89 0.85 0.92 0.89 0.46 0.44 0.18 0.11 0.12 0.67 0.67 0.77 0.67 0.63 0.59
Nucleus 0 0 0 0 0 0.06 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0.05 0 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.15 0.15 0 0.05 0 0.05 0.06 0 0.05
SpindlePole 0 0 0.09 0 0.06 0 0.09 0 0 0 0 0 0.05 0.2 0.2 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.21 0.24 0 0.13 0.11 0.06 0 0 0 0 0.13 0.18 0.16 0.15 0.21 0.06 0.08 0 0.06 0.13 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 1 3 0 0 0 1 1 1 1 1 3 7 7 6 3 0 0 0 4
Bud 9 3 0 2 0 0 8 2 8 5 1 1 2 11 10 9 1 3 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 4 0 0 1 1
Bud Site 2 0 0 0 0 0 1 0 4 4 2 1 0 0 2 1
Cell Periphery 2 8 4 2 1 2 1 1 0 0 0 3 1 32 18 22 0 0 0 0 0 0
Cytoplasm 7 7 2 10 15 11 20 5 7 9 6 14 16 35 29 30 1 1 0 1 0 0
Endoplasmic Reticulum 7 10 4 3 6 4 5 3 1 2 1 2 0 18 9 8 3 1 0 0 0 0
Endosome 6 15 15 3 11 3 10 0 1 0 2 17 19 50 54 29 25 21 15 2 5 9
Golgi 3 20 8 2 11 0 0 0 0 0 0 13 7 44 58 66 7 8 3 2 0 7
Mitochondria 214 139 55 60 91 109 200 129 147 161 168 41 45 63 33 34 183 175 135 28 56 72
Nucleus 8 3 5 2 1 9 9 5 12 7 10 0 1 9 2 5 0 0 0 0 2 0
Nuclear Periphery 8 4 4 1 0 10 12 6 12 14 13 0 1 0 0 0 3 1 1 0 1 2
Nucleolus 5 3 0 1 3 4 5 9 3 9 5 0 1 2 3 0 1 0 0 0 0 1
Peroxisomes 4 10 2 1 2 0 7 0 0 0 2 4 4 53 43 12 13 10 9 2 3 6
SpindlePole 5 5 10 1 8 2 22 3 6 1 6 2 5 68 59 57 3 1 0 0 1 1
Vac/Vac Membrane 34 44 28 2 19 15 16 4 6 2 5 12 18 54 43 59 17 21 4 2 11 10
Unique Cell Count 245 208 116 78 143 140 257 145 172 175 188 89 102 344 292 285 276 261 175 43 90 122
Labelled Cell Count 314 272 139 91 171 169 316 167 208 215 222 111 121 442 372 340 276 261 175 43 90 122


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.4 6.4 5.6 6.5 5.4 5.5 4.8 4.8 4.5 4.6 6.8 6.3 7.3 7.3 7.3 6.0 6.4
Std Deviation (1e-4) 0.7 1.0 0.9 1.1 1.4 1.2 1.1 1.4 0.9 1.4 0.8 1.5 1.1 1.3 1.2 1.5 1.6 1.2
Intensity Change (Log2) -0.18 0.03 -0.23 -0.21 -0.4 -0.4 -0.51 -0.48 0.1 -0.03 0.19 0.2 0.2 -0.09 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2.0 1.1 1.6
Cytoplasm 0 2.8 2.2 2.3 0 0 0 0 3.7 3.7 2.9 2.8 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.8 0 0
Endosome -2.1 -1.4 -3.4 -3.2 0 0 0 0 1.2 1.2 0.4 1.4 -0.8
Golgi 0 0.2 0 0 0 0 0 0 1.8 0 1.7 3.2 3.8
Mitochondria 4.1 2.6 5.1 5.8 0 0 0 0 -0.2 -0.5 -6.2 -8.0 -7.8
Nucleus 0 0 0.7 0 0 0.9 0 0.4 0 0 0 0 0
Nuclear Periphery 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 3.9 3.8 0
SpindlePole 0 -1.0 -2.7 0 0 0 0 0 -1.9 -1.1 2.8 2.8 2.8
Vacuole -4.1 -2.3 -2.9 -5.0 -5.2 -5.3 -6.3 -5.8 -1.9 -1.2 -2.1 -2.3 -0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5936 1.9299 1.8093 2.163 1.9738 1.717 2.2328 2.3488 2.3845 2.4742 1.8257 2.1554 1.759 1.9726 1.6424 1.7957 1.9669 1.5607
Actin 0.0063 0.0078 0.0079 0.0267 0.0191 0.0015 0.0509 0.01 0.0129 0.0832 0.0158 0.0125 0.0201 0.0164 0.0164 0.0137 0.1142 0.0056
Bud 0.0007 0.0014 0.0007 0.0004 0.0008 0.0003 0.0006 0.0004 0.001 0.0014 0.0034 0.0006 0.0004 0.0015 0.0019 0.0009 0.0006 0.0002
Bud Neck 0.0006 0.0004 0.0007 0.0038 0.001 0.0006 0.0145 0.0004 0.0005 0.002 0.001 0.0003 0.0004 0.0007 0.0005 0.0002 0.0026 0.0031
Bud Periphery 0.0016 0.0031 0.003 0.0019 0.0021 0.0007 0.0011 0.0009 0.0015 0.0022 0.0099 0.0023 0.0008 0.0021 0.0033 0.0029 0.0021 0.0004
Bud Site 0.0036 0.0059 0.0021 0.0007 0.0034 0.0008 0.0083 0.0104 0.0036 0.0019 0.0117 0.0004 0.0011 0.0191 0.0098 0.0008 0.0008 0.0003
Cell Periphery 0.0005 0.0004 0.0004 0.0001 0.0007 0.0002 0.0007 0.0003 0.0004 0.0003 0.0007 0.0003 0.0002 0.0013 0.0006 0.0002 0.0003 0.0003
Cytoplasm 0.0016 0.0015 0.0029 0.0004 0.0054 0.0021 0.0056 0.0026 0.0046 0.0057 0.0067 0.0021 0.0027 0.0019 0.0022 0.0026 0.0002 0.0003
Cytoplasmic Foci 0.0276 0.0313 0.0288 0.0249 0.0244 0.0114 0.032 0.0236 0.0284 0.0189 0.0123 0.0052 0.0226 0.038 0.0177 0.0173 0.0029 0.0116
Eisosomes 0.0004 0.0002 0.0002 0.0002 0.0003 0.0001 0.0009 0.0003 0.0003 0.0004 0.0002 0.0004 0.0006 0.0007 0.0003 0.0002 0.0019 0.0002
Endoplasmic Reticulum 0.0023 0.0007 0.0012 0.0004 0.0087 0.0018 0.004 0.0007 0.0022 0.0011 0.0014 0.005 0.0015 0.0007 0.0003 0.0024 0.0023 0.0003
Endosome 0.0754 0.0882 0.0624 0.0242 0.0923 0.0364 0.0712 0.0628 0.0718 0.0821 0.0349 0.0445 0.044 0.0514 0.0255 0.0293 0.028 0.039
Golgi 0.0489 0.0634 0.0516 0.0139 0.0748 0.0291 0.0312 0.0585 0.0375 0.0367 0.0086 0.0338 0.0431 0.0679 0.034 0.0307 0.0704 0.0605
Lipid Particles 0.0564 0.0305 0.0167 0.0031 0.0699 0.031 0.0635 0.0313 0.0273 0.0584 0.0023 0.0232 0.0751 0.04 0.0364 0.0682 0.0084 0.0567
Mitochondria 0.6966 0.7171 0.7686 0.8484 0.6024 0.834 0.6318 0.7528 0.7477 0.6021 0.769 0.8429 0.6714 0.6534 0.7781 0.7164 0.7256 0.7811
None 0.0077 0.0011 0.0051 0.0006 0.0021 0.0065 0.0263 0.0009 0.01 0.0006 0.0044 0.0029 0.0149 0.0042 0.0004 0.0023 0.0008 0.0001
Nuclear Periphery 0.0029 0.0005 0.0007 0.0002 0.004 0.0056 0.0079 0.0024 0.0009 0.0015 0.0066 0.0031 0.0097 0.0008 0.0003 0.0359 0.0143 0.0002
Nucleolus 0.0011 0.0006 0.0004 0.0001 0.0028 0.0003 0.0005 0.0003 0.0003 0.0003 0.0111 0.0002 0.0012 0.001 0.0004 0.0004 0.0003 0.0006
Nucleus 0.0008 0.0004 0.0004 0.0001 0.0019 0.0006 0.0014 0.0002 0.0003 0.0004 0.0301 0.0006 0.0019 0.0004 0.0003 0.0098 0.0006 0.0002
Peroxisomes 0.0474 0.0299 0.0382 0.0473 0.0696 0.0258 0.0353 0.0301 0.0277 0.0894 0.0109 0.0097 0.0604 0.066 0.0539 0.0581 0.0174 0.0305
Punctate Nuclear 0.0008 0.0002 0.0007 0.0002 0.0015 0.0003 0.0044 0.0002 0.0002 0.001 0.0034 0.0003 0.0106 0.0002 0.0044 0.0051 0.001 0.0001
Vacuole 0.0086 0.0084 0.0037 0.001 0.0073 0.0038 0.0035 0.0048 0.0088 0.004 0.0517 0.0035 0.0029 0.0182 0.011 0.001 0.0012 0.0036
Vacuole Periphery 0.0083 0.0067 0.0036 0.0013 0.0056 0.0069 0.0044 0.006 0.0122 0.0063 0.0038 0.0062 0.0146 0.0142 0.0024 0.0016 0.004 0.0049

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.2108 9.1173 12.6852 20.1298 16.9425 24.8463 25.927 21.3876 28.6208 28.1282
Translational Efficiency 0.4221 0.611 0.4519 0.3562 0.3633 0.4518 0.3578 0.4676 0.435 0.3511

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
246 462 1574 159 1280 1772 1886 172 1526 2234 3460 331

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.28 769.49 888.86 939.16 684.64 794.23 859.30 908.16 676.84 789.11 872.75 923.05
Standard Deviation 72.46 126.01 123.33 130.22 94.51 114.65 112.24 122.23 93.03 117.52 118.33 127.08
Intensity Change Log 2 0.274241 0.482294 0.561709 0.214211 0.327816 0.407601 0.243439 0.404294 0.483891

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001578 0.007081 0.015057 0.006930 0.005282 0.003987 0.020395 0.019631 0.004685 0.004627 0.017967 0.013530
Bud Neck 0.048664 0.015276 0.018008 0.022701 0.013766 0.015865 0.036825 0.023365 0.019392 0.015743 0.028265 0.023046
Bud Site 0.010191 0.045628 0.073728 0.087129 0.015083 0.024711 0.107175* 0.082590 0.014294 0.029037 0.091960 0.084770
Cell Periphery 0.003656 0.001181 0.000467 0.000510 0.001759 0.000582 0.000891 0.000362 0.002065 0.000706 0.000698 0.000433
Cytoplasm 0.191673* 0.017634 0.025649 0.047986 0.039542 0.017287 0.091140 0.038987 0.064066 0.017359 0.061347 0.043309
Cytoplasmic Foci 0.278464* 0.098552 0.090360 0.158710 0.142623 0.093680 0.127603 0.133030 0.164521 0.094688 0.110661 0.145366
Eisosomes 0.003536 0.000370 0.000167 0.000199 0.000787 0.000185 0.000606 0.000191 0.001230 0.000223 0.000406 0.000195
Endoplasmic Reticulum 0.005402 0.001655 0.007990 0.008436 0.002111 0.001347 0.004500 0.004621 0.002641 0.001411 0.006088 0.006453
Endosome 0.031614 0.146516 0.110638 0.121354 0.059905 0.154551 0.089701 0.134183 0.055344 0.152889 0.099226 0.128021
Golgi 0.057203 0.129786* 0.081051 0.099441 0.153637* 0.146749* 0.076308 0.078903 0.138092* 0.143241* 0.078465 0.088769
Lipid Particles 0.072155 0.011211 0.010170 0.008478 0.027596 0.013168 0.013108 0.015519 0.034779 0.012764 0.011772 0.012137
Mitochondria 0.151187* 0.365627* 0.248668* 0.107422* 0.424980* 0.392302* 0.177128* 0.100334* 0.380843* 0.386786* 0.209672* 0.103739*
Mitotic Spindle 0.007623 0.016892 0.116806* 0.092689 0.008302 0.021611 0.053682 0.124307* 0.008192 0.020635 0.082397 0.109119*
None 0.002791 0.000384 0.000842 0.002110 0.001352 0.000815 0.000958 0.000376 0.001584 0.000725 0.000905 0.001209
Nuclear Periphery 0.000234 0.000804 0.001403 0.000838 0.000389 0.000368 0.000572 0.000796 0.000364 0.000458 0.000950 0.000816
Nuclear Periphery Foci 0.000319 0.001115 0.004393 0.002591 0.000904 0.000746 0.001794 0.002847 0.000810 0.000822 0.002976 0.002724
Nucleolus 0.001927 0.001303 0.003280 0.002034 0.001863 0.001326 0.001524 0.000738 0.001873 0.001321 0.002323 0.001360
Nucleus 0.036575 0.002309 0.017897 0.014818 0.007701 0.002628 0.022540 0.012173 0.012355 0.002562 0.020428 0.013443
Peroxisomes 0.079070 0.097124 0.059909 0.058340 0.072897 0.042457 0.087397 0.075928 0.073892 0.053763 0.074893 0.067480
Vacuole 0.012060 0.024033 0.088878 0.137180 0.009664 0.042216 0.078111 0.129384 0.010051 0.038455 0.083009 0.133129
Vacuole Periphery 0.004078 0.015519 0.024639 0.020105 0.009858 0.023418 0.008045 0.021736 0.008926 0.021785 0.015593 0.020952

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.02 -9.01 -4.16 0.07 4.23 1.16 -7.68 -2.40 -2.66 0.26 0.05 -9.54 -2.76 -2.81 1.54
Bud Neck 3.89 3.59 2.93 -1.50 -0.83 -1.01 -8.71 -1.82 -1.43 2.50 1.61 -3.91 -0.86 -2.02 1.72
Bud Site -6.22 -16.45 -5.89 -2.85 -0.80 -4.52 -21.47 -5.88 -4.99 2.08 -7.07 -26.36 -8.14 -6.30 1.00
Cell Periphery 2.75 3.66 3.63 2.81 -0.17 1.99 1.49 2.41 2.11 6.77 2.63 2.68 3.23 2.79 4.64
Cytoplasm 11.42 10.91 7.01 -3.45 -2.70 6.42 -11.06 -0.33 -2.71 5.15 11.51 -0.01 1.62 -4.42 1.73
Cytoplasmic Foci 12.77 14.30 6.75 -3.94 -4.89 9.32 2.67 0.88 -3.10 -0.29 13.67 10.94 2.23 -5.23 -3.55
Eisosomes 6.57 7.10 7.01 1.78 -1.05 4.61 1.12 4.50 -0.41 3.83 7.33 5.61 7.51 0.81 3.40
Endoplasmic Reticulum 1.21 -1.05 -0.52 -1.53 0.15 2.00 -3.74 -2.48 -3.31 -0.17 2.08 -5.24 -1.73 -2.39 0.04
Endosome -12.40 -13.68 -7.12 2.42 -0.48 -18.37 -6.86 -5.20 2.21 -2.97 -21.42 -12.48 -7.55 3.63 -2.67
Golgi -7.16 -3.18 -2.23 2.68 -0.75 0.97 12.65 7.20 6.90 0.14 -0.85 11.70 6.16 7.04 -0.50
Lipid Particles 8.01 8.50 8.49 0.63 0.62 5.40 5.52 2.17 -0.46 -0.42 8.50 9.29 6.15 0.08 -0.13
Mitochondria -9.50 -5.35 2.23 10.69 7.16 2.57 21.61 17.86 16.96 4.68 -0.53 17.27 18.82 20.57 8.33
Mitotic Spindle -1.85 -16.65 -5.19 -4.70 1.55 -6.81 -13.62 -6.08 -5.34 -3.60 -6.90 -23.96 -8.05 -7.01 -1.96
None 2.46 1.94 0.56 -1.36 -0.94 1.28 0.72 2.53 2.13 2.08 2.26 1.63 0.69 -0.69 -0.32
Nuclear Periphery -1.46 -6.88 -2.94 -0.08 2.32 0.09 -0.94 -1.40 -1.72 -0.94 -0.50 -3.29 -2.04 -1.97 0.66
Nuclear Periphery Foci -2.14 -5.82 -5.14 -2.86 2.09 0.34 -2.08 -2.05 -2.31 -1.13 -0.07 -4.76 -3.29 -3.65 0.30
Nucleolus 0.97 -2.19 0.13 -0.70 2.01 0.90 0.39 1.98 1.74 2.73 1.09 -1.12 1.10 0.10 2.82
Nucleus 7.20 3.08 2.97 -3.24 0.71 4.53 -8.01 -1.77 -3.10 2.04 7.88 -5.63 -1.14 -4.48 1.94
Peroxisomes -1.64 2.29 1.81 3.23 0.31 6.84 -2.55 -0.06 -3.24 1.27 4.71 -0.04 1.15 -1.59 1.23
Vacuole -3.03 -17.42 -8.22 -7.58 -3.83 -14.42 -20.93 -8.01 -6.19 -3.86 -14.53 -29.46 -11.58 -9.33 -5.46
Vacuole Periphery -6.20 -12.78 -3.91 -0.66 1.61 -9.67 2.17 -2.06 0.68 -2.48 -10.78 -6.66 -3.28 0.85 -1.24
Standard name
Human Ortholog
Description Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)
Localization
Cell Percentages mitochondrion (46%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Msk1

Msk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available