Standard name
Human Ortholog
Description Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.3 0.31 0.27 0.34 0.33 0.4 0.5 0.41 0.43 0.4 0.44 0.36 0.43 0.51 0.14 0.15 0.15 0.23 0.14 0.14 0.3 0.31 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.2 0 0.12 0.2 0.24 0.22 0.2 0.56 0.38 0.5 0.5 0 0 0 0.09 0 0 0 0 0 0 0.05 0.05
Nucleus 0.49 0.5 0.65 0.58 0.53 0.46 0.4 0.29 0.24 0.28 0.26 0.19 0.58 0.51 0.39 0.71 0.73 0.74 0.4 0.53 0.53 0.29 0.29 0.15
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0
Vac/Vac Membrane 0.16 0.12 0.05 0.08 0 0.08 0.06 0.07 0 0 0 0 0.09 0.09 0.14 0.15 0.12 0.09 0.23 0.19 0.2 0.27 0.25 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 2 2
Bud 1 0 0 0 2 2 4 5 9 4 5 5 0 0 0 0 0 0 11 11 6 2 2 4
Bud Neck 0 0 3 1 4 5 3 5 5 9 3 7 0 2 0 0 0 0 3 1 2 1 1 3
Bud Site 0 0 0 0 3 5 5 1 10 3 2 6 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 142 165 84 84 112 137 197 274 240 209 220 229 76 151 193 33 49 28 116 95 50 81 87 113
Endoplasmic Reticulum 0 3 1 0 1 1 2 1 0 0 6 2 0 0 0 11 20 13 1 2 0 1 1 3
Endosome 0 0 0 3 0 6 1 2 0 5 0 0 1 7 3 6 6 3 1 5 3 1 2 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 2 3 1 0 0 0
Mitochondria 29 111 4 37 64 97 107 112 328 183 277 260 0 0 3 20 12 7 10 19 6 11 13 14
Nucleus 184 274 177 183 172 190 200 162 140 136 146 102 122 179 150 165 241 142 205 349 192 79 81 41
Nuclear Periphery 0 1 1 1 0 0 2 0 0 1 0 0 0 0 1 1 1 0 0 0 0 2 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 2 1 1 0 0 0 0 0 1 0 2 1 2 2 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 1 3 3 4 2 2 10 18 5 13 10 4 0 1 1 1 5 0 23 32 11 6 9 11
Vac/Vac Membrane 60 67 13 24 10 33 29 39 10 8 9 17 18 31 54 35 39 18 117 125 71 73 70 65
Unique Cell Count 377 546 271 313 327 411 494 550 582 484 554 524 209 352 380 234 330 193 508 663 363 271 281 278
Labelled Cell Count 419 625 286 338 370 478 560 619 749 574 679 633 217 372 405 273 376 213 508 663 363 271 281 278


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 5.1 4.9 4.4 4.2 4.2 4.4 3.4 3.7 3.4 3.5 5.1 5.2 4.8 5.1 6.2 6.1 5.4 5.4 5.8
Std Deviation (1e-4) 0.6 0.7 1.0 1.4 1.3 0.8 1.1 1.3 1.1 0.7 0.5 0.5 0.8 1.2 1.0 1.3 1.7 1.4 1.8 1.3 1.8
Intensity Change (Log2) – – – -0.07 -0.22 -0.29 -0.26 -0.2 -0.6 -0.48 -0.57 -0.55 -0.01 0.03 -0.08 -0.01 0.28 0.26 0.09 0.09 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 3.0 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4857 2.1747 2.0779 1.5234 1.6112 2.1402 4.8234 4.5508 6.1265 4.7658 4.4231 4.9994 2.7518 3.0884 2.9528 2.7996 2.9584 3.0067
Actin 0.0182 0.0003 0.0015 0.0006 0.0006 0.0002 0.0056 0 0.0281 0.0009 0 0.0005 0.0066 0 0.0036 0.0002 0.0001 0.0001
Bud 0.0008 0.0001 0.0002 0.0001 0 0.0001 0.0003 0 0.0005 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Bud Neck 0.0019 0.0006 0.0031 0.0009 0.0006 0.0046 0.0006 0.0002 0.0002 0.0002 0.0006 0.0233 0.0006 0.0002 0.0003 0.0002 0.0003 0.0014
Bud Periphery 0.002 0.0002 0.0007 0.0003 0 0.0003 0.0003 0 0.0009 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Bud Site 0.0067 0.0006 0.0084 0.0002 0.0001 0.0005 0.0007 0.0003 0.0011 0.0004 0.0003 0.0003 0.0006 0.0002 0.0003 0 0 0
Cell Periphery 0.0007 0.0001 0.0002 0.0001 0 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0.0287 0.0136 0.0041 0.006 0.0112 0.0178 0.0593 0.022 0.0077 0.0054 0.001 0.0274 0.0069 0.0169 0.0049 0.0105 0.0042 0.011
Cytoplasmic Foci 0.0029 0.0005 0.0028 0.0015 0.0017 0.002 0.0244 0.0007 0.0011 0.0141 0 0.0035 0.0044 0.0009 0.0011 0.0003 0.0003 0.0003
Eisosomes 0.0014 0 0 0.0001 0 0.0001 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0027 0.0006 0.0008 0.0003 0.0007 0.0005 0.0047 0.0007 0.0036 0.0004 0.0001 0.0027 0.001 0.0005 0.0005 0.0002 0.0004 0.0003
Endosome 0.0055 0.0008 0.0049 0.0235 0.0052 0.0042 0.0248 0.0012 0.0105 0.0036 0.0001 0.012 0.0069 0.0012 0.0021 0.0003 0.0004 0.0005
Golgi 0.0031 0.0002 0.0034 0.0107 0.013 0.0018 0.0045 0 0.0061 0.005 0 0.0013 0.0047 0 0.0009 0 0 0
Lipid Particles 0.004 0.0005 0.0059 0.0017 0.0106 0.0032 0.0213 0.0004 0.0012 0.061 0 0.0004 0.0078 0.0001 0.0017 0.0001 0.0002 0.0001
Mitochondria 0.0171 0.0162 0.0036 0.0282 0.0156 0.0014 0.0017 0.0001 0.0049 0.0017 0.0003 0.0009 0.0021 0.0001 0.0008 0.0002 0.0004 0.0002
None 0.0635 0.0141 0.0253 0.133 0.067 0.0723 0.1488 0.1752 0.0457 0.1005 0.0002 0.065 0.022 0.0364 0.0176 0.036 0.023 0.0252
Nuclear Periphery 0.0097 0.0105 0.0171 0.0027 0.01 0.0022 0.0413 0.0043 0.0233 0.0119 0.004 0.0043 0.0166 0.0085 0.0088 0.01 0.0123 0.005
Nucleolus 0.0141 0.0106 0.0098 0.0023 0.0102 0.0196 0.0038 0.0041 0.0054 0.0054 0.0138 0.0164 0.0083 0.0074 0.0073 0.0072 0.0094 0.0179
Nucleus 0.7901 0.9166 0.8977 0.77 0.8235 0.8585 0.6348 0.7848 0.8501 0.7505 0.9765 0.83 0.8797 0.9076 0.9312 0.9027 0.9184 0.919
Peroxisomes 0.0009 0.0006 0.0008 0.0004 0.0011 0.0006 0.0037 0 0.0004 0.0158 0 0.0002 0.0032 0 0.0026 0 0 0
Punctate Nuclear 0.0223 0.0121 0.008 0.0079 0.0273 0.0083 0.0136 0.003 0.0034 0.0221 0.0014 0.0096 0.0271 0.0186 0.0155 0.0318 0.0302 0.0186
Vacuole 0.0027 0.0009 0.0011 0.0054 0.0009 0.0016 0.0042 0.0026 0.002 0.0004 0.0015 0.0017 0.0007 0.0012 0.0003 0.0001 0.0001 0.0003
Vacuole Periphery 0.0011 0.0004 0.0006 0.004 0.0005 0.0002 0.0013 0.0001 0.0036 0.0003 0.0001 0.0003 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.2277 22.4176 22.1798 19.3649 17.0311 33.418 15.111 15.6626 20.3443 28.5694
Translational Efficiency 1.912 1.3066 0.9399 1.7625 3.0202 1.9645 1.3015 1.234 1.0634 1.3604

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1745 1313 3022 1339 2146 1832 1867 1395 3891 3145 4889 2734

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 716.45 756.52 802.82 807.86 649.19 822.59 814.61 830.78 679.35 795.01 807.32 819.55
Standard Deviation 81.70 95.62 119.60 121.67 76.43 98.49 108.12 114.87 85.64 102.61 115.49 118.80
Intensity Change Log 2 — 0.078512 0.164211 0.173239 — 0.341533 0.327469 0.355826 — 0.209534 0.244126 0.262922

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000041 0.000497 0.000222 0.000793 0.000042 0.000543 0.000603 0.000619 0.000042 0.000524 0.000368 0.000704
Bud Neck 0.004449 0.018709 0.015884 0.011136 0.004708 0.016145 0.010537 0.019017 0.004592 0.017215 0.013842 0.015157
Bud Site 0.000857 0.009505 0.002456 0.010340 0.000691 0.007728 0.005046 0.023063 0.000765 0.008470 0.003445 0.016832
Cell Periphery 0.000125 0.000290 0.000169 0.000182 0.000138 0.000280 0.000136 0.000209 0.000132 0.000284 0.000157 0.000196
Cytoplasm 0.260064* 0.208678* 0.030682 0.108467* 0.272023* 0.215933* 0.025073 0.104394* 0.266660* 0.212904* 0.028541 0.106389*
Cytoplasmic Foci 0.041469 0.072257 0.000156 0.002641 0.031839 0.059297 0.000462 0.004786 0.036158 0.064708 0.000273 0.003735
Eisosomes 0.000034 0.000075 0.000013 0.000031 0.000031 0.000070 0.000016 0.000030 0.000032 0.000072 0.000014 0.000030
Endoplasmic Reticulum 0.000761 0.004216 0.003232 0.004565 0.000541 0.001707 0.003826 0.005780 0.000640 0.002755 0.003459 0.005185
Endosome 0.001185 0.006023 0.000323 0.002791 0.001290 0.006379 0.000450 0.003673 0.001243 0.006230 0.000372 0.003241
Golgi 0.000253 0.002287 0.000030 0.002027 0.000139 0.002209 0.000197 0.002482 0.000190 0.002242 0.000094 0.002260
Lipid Particles 0.003284 0.005806 0.000096 0.000722 0.002599 0.006042 0.000144 0.000568 0.002906 0.005944 0.000115 0.000643
Mitochondria 0.003334 0.010774 0.001338 0.005369 0.003744 0.008618 0.001884 0.007825 0.003560 0.009518 0.001547 0.006622
Mitotic Spindle 0.001147 0.006585 0.002017 0.021128 0.002048 0.008510 0.003077 0.019942 0.001644 0.007706 0.002422 0.020523
None 0.005746 0.007427 0.004384 0.012892 0.008778 0.009752 0.004680 0.006107 0.007418 0.008782 0.004497 0.009430
Nuclear Periphery 0.001241 0.001974 0.001366 0.002455 0.000922 0.001605 0.001033 0.002396 0.001065 0.001759 0.001238 0.002425
Nuclear Periphery Foci 0.000255 0.001566 0.000150 0.000859 0.000172 0.000630 0.000114 0.000937 0.000209 0.001021 0.000136 0.000899
Nucleolus 0.001966 0.005614 0.000921 0.001533 0.002196 0.005776 0.001243 0.001095 0.002093 0.005709 0.001044 0.001309
Nucleus 0.665619* 0.604444* 0.932791* 0.783075* 0.661409* 0.619526* 0.937336* 0.763629* 0.663297* 0.613230* 0.934527* 0.773152*
Peroxisomes 0.001405 0.008789 0.000033 0.000509 0.001331 0.007034 0.000130 0.000717 0.001364 0.007767 0.000070 0.000615
Vacuole 0.006359 0.023333 0.003524 0.026674 0.005206 0.020523 0.003874 0.029706 0.005723 0.021696 0.003657 0.028221
Vacuole Periphery 0.000408 0.001153 0.000212 0.001812 0.000152 0.001693 0.000138 0.003023 0.000267 0.001468 0.000184 0.002430

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.36 -16.18 -6.66 -1.67 -4.54 -1.77 -2.96 -10.85 -0.43 -0.33 -2.95 -4.73 -11.30 -0.87 -3.27
Bud Neck -8.23 -10.66 -6.77 3.45 3.65 -8.33 -7.12 -10.86 -1.68 -4.05 -11.69 -13.03 -12.69 1.15 -0.11
Bud Site -7.31 -5.50 -6.61 1.20 -4.68 -7.27 -4.31 -8.30 -4.12 -5.50 -10.28 -6.55 -10.40 -2.77 -7.44
Cell Periphery -5.51 -1.82 -1.44 4.99 0.82 -5.54 0.63 -1.62 4.06 -2.05 -7.82 -1.05 -2.09 6.46 -0.78
Cytoplasm 5.99 37.35 22.32 14.60 -14.03 7.01 41.15 23.34 15.62 -15.43 9.17 55.77 32.39 21.56 -20.90
Cytoplasmic Foci -6.46 17.52 16.59 17.77 -6.01 -8.70 18.84 16.22 20.34 -4.23 -10.53 25.58 23.25 27.00 -6.57
Eisosomes -10.24 11.10 -4.58 7.12 -16.19 -8.81 7.09 -4.75 6.33 -12.37 -12.98 13.59 -6.68 9.10 -21.03
Endoplasmic Reticulum -13.15 -20.15 -20.56 -6.30 -9.93 -7.77 -16.33 -20.40 -16.06 -8.25 -14.91 -26.40 -28.76 -16.88 -13.15
Endosome -7.06 5.64 -2.43 5.76 -6.29 -7.64 2.19 -2.38 6.11 -5.99 -10.44 4.03 -3.19 8.51 -8.70
Golgi -4.85 3.70 -1.41 2.35 -1.96 -5.02 -0.23 -2.40 1.80 -2.29 -6.88 2.32 -2.72 2.90 -3.08
Lipid Particles -3.49 5.68 4.30 10.28 -11.14 -6.69 9.93 7.30 11.78 -12.58 -6.98 9.79 7.27 15.59 -16.84
Mitochondria -4.70 2.43 0.18 4.88 -2.29 -3.84 1.70 -0.85 2.91 -2.74 -6.04 2.94 -0.54 5.61 -3.75
Mitotic Spindle -3.68 -1.97 -6.70 -4.18 -6.11 -3.71 -1.06 -6.46 -3.71 -5.89 -5.17 -1.87 -9.27 -5.50 -8.58
None -2.14 3.31 -2.20 -0.69 -3.69 -0.90 4.84 4.12 4.40 -1.50 -2.01 6.46 0.50 2.10 -4.12
Nuclear Periphery -3.77 -2.84 -7.31 -4.42 -6.31 -2.97 -5.72 -15.48 -7.22 -12.49 -4.42 -6.08 -14.67 -8.09 -12.08
Nuclear Periphery Foci -2.48 0.86 -4.10 0.91 -4.43 -2.59 1.39 -4.48 -1.79 -4.68 -3.34 0.94 -6.12 -0.26 -6.32
Nucleolus -4.84 2.96 1.77 6.37 -2.27 -4.25 2.46 2.55 6.53 0.25 -6.29 3.91 3.08 9.01 -1.51
Nucleus 5.45 -35.52 -10.68 -14.55 19.64 4.31 -36.76 -8.00 -11.17 21.64 6.81 -51.80 -13.21 -18.14 29.43
Peroxisomes -7.16 6.22 4.22 8.43 -2.93 -7.15 4.51 3.06 8.67 -3.08 -10.11 7.37 4.98 12.09 -4.47
Vacuole -10.74 2.74 -13.44 -7.96 -13.87 -10.16 -1.39 -15.45 -10.74 -14.86 -14.66 0.76 -20.45 -13.34 -20.49
Vacuole Periphery -4.15 1.63 -1.43 0.91 -2.22 -3.10 0.51 -2.26 0.30 -2.29 -4.00 1.46 -2.70 0.67 -2.99
Standard name
Human Ortholog
Description Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
Localization
Cell Percentages nucleus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rnh201

Rnh201


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnh201-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available