ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 10 | 6 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 1 | 3 | 2 | 8 | 7 | 14 | 5 | 21 | 16 | 22 | 19 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 9 | 33 | 10 | 15 | 25 | 37 | 41 | 75 | 27 | 53 | 63 | 9 | 12 | 24 | 10 | 10 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 625 | 318 | 594 | 313 | 416 | 471 | 527 | 454 | 510 | 483 | 402 | 460 | 577 | 412 | 589 | 149 | 194 | 170 | 615 | 310 | 589 | 338 | 513 | 574 |
Endoplasmic Reticulum | 3 | 2 | 8 | 4 | 13 | 16 | 26 | 25 | 35 | 52 | 11 | 26 | 2 | 1 | 2 | 7 | 6 | 6 | 3 | 1 | 6 | 0 | 1 | 2 |
Endosome | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 2 | 5 | 3 | 4 | 0 | 0 | 3 |
Golgi | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 1 | 6 |
Mitochondria | 5 | 2 | 5 | 30 | 67 | 152 | 241 | 197 | 230 | 346 | 332 | 301 | 0 | 1 | 1 | 2 | 5 | 3 | 2 | 2 | 4 | 0 | 1 | 5 |
Nucleus | 2 | 6 | 0 | 0 | 6 | 2 | 4 | 7 | 4 | 7 | 4 | 3 | 2 | 1 | 0 | 2 | 7 | 2 | 2 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 12 | 0 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 0 | 11 | 10 | 13 | 16 | 66 | 91 | 78 | 81 | 79 | 91 | 1 | 2 | 1 | 7 | 1 | 4 | 2 | 9 | 2 | 2 | 2 | 1 |
Unique Cell Count | 635 | 324 | 614 | 337 | 486 | 588 | 771 | 714 | 777 | 839 | 720 | 802 | 580 | 417 | 595 | 158 | 202 | 182 | 644 | 337 | 623 | 346 | 529 | 600 |
Labelled Cell Count | 652 | 338 | 655 | 371 | 543 | 693 | 922 | 839 | 964 | 1026 | 911 | 972 | 592 | 431 | 621 | 179 | 223 | 202 | 644 | 337 | 623 | 346 | 529 | 600 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 9.8 | 8.8 | 8.3 | 8.2 | 7.8 | 7.6 | 7.7 | 7.8 | 7.1 | 6.7 | 7.3 | 9.8 | 10.1 | 9.4 | 9.5 | 8.5 | 9.4 | 11.1 | 11.4 | 11.9 |
Std Deviation (1e-4) | 1.1 | 1.9 | 1.3 | 1.3 | 1.5 | 1.6 | 1.6 | 1.6 | 1.5 | 1.5 | 1.5 | 1.6 | 1.4 | 1.3 | 1.4 | 1.7 | 1.5 | 1.8 | 1.7 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.1 | -0.17 | -0.2 | -0.18 | -0.17 | -0.3 | -0.38 | -0.27 | 0.16 | 0.21 | 0.11 | 0.12 | -0.04 | 0.1 | 0.34 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.2 | 0 | 3.1 | 2.4 | 3.4 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.7 | -1.8 | -0.9 | -0.5 | 0.3 | 3.0 | -2.0 | 1.5 | 1.8 | -3.6 | -1.9 | -1.1 | 0.5 | -0.2 | 0.3 |
Cytoplasm | -2.7 | -6.7 | -9.1 | -13.4 | -14.8 | -14.2 | -16.8 | -17.1 | -16.8 | 3.4 | 2.1 | 2.7 | -1.4 | -0.5 | -2.0 |
Endoplasmic Reticulum | 0 | 1.7 | 1.8 | 2.5 | 2.6 | 3.4 | 4.6 | 0.3 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.3 | 8.6 | 12.9 | 14.7 | 13.5 | 14.2 | 17.8 | 19.0 | 16.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.2 | 1.0 | 1.1 | 5.5 | 7.5 | 6.2 | 6.1 | 6.7 | 6.9 | -2.8 | -1.9 | -2.8 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3697 | 5.9211 | 6.3544 | 5.3927 | 5.0311 | 6.0363 | 5.0385 | 5.9576 | 5.7665 | 4.2842 | 5.0694 | 5.3945 | 3.773 | 5.4698 | 4.8086 | 4.2551 | 3.82 | 4.9156 |
Actin | 0.0177 | 0.0003 | 0.0011 | 0.0014 | 0.0001 | 0.0003 | 0.0046 | 0.0003 | 0.0013 | 0.0016 | 0.0025 | 0.0021 | 0.0007 | 0.0002 | 0.0007 | 0.0006 | 0.0003 | 0.0003 |
Bud | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.002 | 0.0019 | 0.0007 | 0.0007 | 0.0001 | 0.0016 | 0.0004 | 0.0002 | 0.0002 | 0.0018 |
Bud Neck | 0.0005 | 0.0009 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0016 | 0.0003 | 0.0004 | 0.0013 | 0.0005 | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0007 |
Bud Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0007 | 0.0015 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0015 | 0.0037 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0007 | 0.0084 | 0.001 | 0.0069 | 0.0008 | 0.0002 | 0.0002 | 0.0008 | 0.0008 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.8139 | 0.8827 | 0.904 | 0.9198 | 0.941 | 0.9234 | 0.8935 | 0.9533 | 0.957 | 0.8965 | 0.8911 | 0.937 | 0.9049 | 0.9654 | 0.9374 | 0.9593 | 0.9243 | 0.9514 |
Cytoplasmic Foci | 0.0132 | 0.0089 | 0.0063 | 0.0088 | 0.0131 | 0.0054 | 0.0121 | 0.0056 | 0.0069 | 0.0212 | 0.0175 | 0.0092 | 0.0083 | 0.0039 | 0.0069 | 0.0075 | 0.01 | 0.006 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0093 | 0.0012 | 0.0052 | 0.0094 | 0.0018 | 0.0052 | 0.0091 | 0.005 | 0.0026 | 0.0027 | 0.0044 | 0.0036 | 0.0091 | 0.0015 | 0.0031 | 0.005 | 0.0034 | 0.0048 |
Endosome | 0.004 | 0.0068 | 0.005 | 0.0165 | 0.0043 | 0.0035 | 0.0149 | 0.0035 | 0.0063 | 0.0172 | 0.0273 | 0.0064 | 0.0101 | 0.0024 | 0.0054 | 0.0094 | 0.0165 | 0.0047 |
Golgi | 0.002 | 0.0006 | 0.001 | 0.0012 | 0.0002 | 0.0003 | 0.0015 | 0.0003 | 0.0009 | 0.002 | 0.0019 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0008 | 0.0003 |
Lipid Particles | 0.0008 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0022 | 0.0001 | 0.0003 | 0.0171 | 0.0028 | 0.0017 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Mitochondria | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0008 | 0.0003 | 0.0006 | 0.0013 | 0.0011 | 0.002 | 0.0002 | 0.0062 | 0.0002 | 0.0003 | 0.0003 | 0.0008 |
None | 0.1309 | 0.084 | 0.0722 | 0.0299 | 0.0338 | 0.0579 | 0.0359 | 0.0177 | 0.0132 | 0.0057 | 0.0185 | 0.027 | 0.0599 | 0.0142 | 0.0405 | 0.0128 | 0.0401 | 0.0257 |
Nuclear Periphery | 0.0009 | 0.0005 | 0.0006 | 0.0025 | 0.0005 | 0.0007 | 0.0024 | 0.0007 | 0.0008 | 0.0012 | 0.0127 | 0.0022 | 0.0012 | 0.0003 | 0.0007 | 0.0007 | 0.0005 | 0.0004 |
Nucleolus | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Nucleus | 0.0008 | 0.003 | 0.0007 | 0.0021 | 0.0015 | 0.0011 | 0.0105 | 0.0016 | 0.0025 | 0.0028 | 0.0038 | 0.0019 | 0.0009 | 0.0009 | 0.0009 | 0.0009 | 0.0007 | 0.0016 |
Peroxisomes | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0008 | 0.0001 | 0.0003 | 0.0115 | 0.0011 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0014 | 0.0033 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0058 | 0.0002 | 0.0005 | 0.0013 | 0.0073 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 |
Vacuole | 0.001 | 0.0026 | 0.0012 | 0.004 | 0.0021 | 0.0007 | 0.0025 | 0.0015 | 0.0021 | 0.005 | 0.0036 | 0.0013 | 0.0023 | 0.0009 | 0.0011 | 0.0014 | 0.0017 | 0.0007 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0011 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.9614 | 21.5837 | 15.4822 | 16.3411 | 17.7392 | 16.0874 | 24.0056 | 21.6029 | 23.4501 | 27.5127 |
Translational Efficiency | 0.6618 | 0.6666 | 0.8942 | 0.9483 | 0.8186 | 1.4692 | 0.915 | 1.0094 | 0.9245 | 0.8077 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2196 | 415 | 2711 | 1290 | 1689 | 805 | 602 | 1286 | 3885 | 1220 | 3313 | 2576 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 937.10 | 1101.31 | 969.96 | 802.16 | 941.07 | 1010.12 | 994.68 | 803.24 | 938.83 | 1041.14 | 974.45 | 802.70 |
Standard Deviation | 121.75 | 173.58 | 155.68 | 117.84 | 128.11 | 138.43 | 155.54 | 133.93 | 124.57 | 157.35 | 155.95 | 126.13 |
Intensity Change Log 2 | — | 0.232946 | 0.049722 | -0.224313 | — | 0.102153 | 0.079930 | -0.228471 | — | 0.168893 | 0.064937 | -0.226395 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000200 | 0.001812 | 0.000110 | 0.000636 | 0.000090 | 0.000808 | 0.000147 | 0.001142 | 0.000152 | 0.001149 | 0.000116 | 0.000889 |
Bud Neck | 0.002067 | 0.001946 | 0.002699 | 0.007772 | 0.001370 | 0.002283 | 0.001210 | 0.009645 | 0.001764 | 0.002168 | 0.002428 | 0.008707 |
Bud Site | 0.016723 | 0.030594 | 0.011171 | 0.030920 | 0.006702 | 0.018166 | 0.003752 | 0.052753 | 0.012366 | 0.022394 | 0.009822 | 0.041820 |
Cell Periphery | 0.000157 | 0.000154 | 0.000052 | 0.000207 | 0.000070 | 0.000147 | 0.000089 | 0.000246 | 0.000119 | 0.000149 | 0.000059 | 0.000226 |
Cytoplasm | 0.756231* | 0.715630* | 0.809450* | 0.479543* | 0.745337* | 0.674812* | 0.683112* | 0.495670* | 0.751495* | 0.688697* | 0.786494* | 0.487594* |
Cytoplasmic Foci | 0.150678 | 0.161248 | 0.016357 | 0.025719 | 0.137995 | 0.176902 | 0.024377 | 0.032703 | 0.145164 | 0.171577 | 0.017814 | 0.029206 |
Eisosomes | 0.000028 | 0.000019 | 0.000008 | 0.000055 | 0.000012 | 0.000031 | 0.000028 | 0.000054 | 0.000021 | 0.000027 | 0.000012 | 0.000054 |
Endoplasmic Reticulum | 0.002641 | 0.000706 | 0.000274 | 0.003530 | 0.003186 | 0.002693 | 0.001071 | 0.000999 | 0.002877 | 0.002017 | 0.000419 | 0.002266 |
Endosome | 0.005950 | 0.010773 | 0.001240 | 0.006267 | 0.008683 | 0.018177 | 0.002830 | 0.014533 | 0.007138 | 0.015659 | 0.001529 | 0.010394 |
Golgi | 0.012964 | 0.005506 | 0.000170 | 0.003604 | 0.004621 | 0.006352 | 0.000171 | 0.010623 | 0.009337 | 0.006065 | 0.000170 | 0.007108 |
Lipid Particles | 0.001362 | 0.001338 | 0.000285 | 0.001709 | 0.001454 | 0.002077 | 0.000887 | 0.003425 | 0.001402 | 0.001826 | 0.000394 | 0.002566 |
Mitochondria | 0.001270 | 0.000865 | 0.000306 | 0.002535 | 0.001523 | 0.003358 | 0.003059 | 0.007332 | 0.001380 | 0.002510 | 0.000806 | 0.004930 |
Mitotic Spindle | 0.000565 | 0.009688 | 0.007663 | 0.025848 | 0.004370 | 0.004393 | 0.004838 | 0.043988 | 0.002219 | 0.006194 | 0.007149 | 0.034904 |
None | 0.002397 | 0.000422 | 0.004233 | 0.006684 | 0.001515 | 0.000570 | 0.004620 | 0.006079 | 0.002014 | 0.000520 | 0.004303 | 0.006382 |
Nuclear Periphery | 0.000036 | 0.000075 | 0.000299 | 0.001780 | 0.000111 | 0.000090 | 0.000653 | 0.001468 | 0.000068 | 0.000085 | 0.000363 | 0.001624 |
Nuclear Periphery Foci | 0.000360 | 0.001497 | 0.000688 | 0.001940 | 0.000489 | 0.001315 | 0.003356 | 0.002002 | 0.000416 | 0.001377 | 0.001173 | 0.001971 |
Nucleolus | 0.000307 | 0.000245 | 0.000210 | 0.000479 | 0.000216 | 0.000259 | 0.000290 | 0.000972 | 0.000268 | 0.000254 | 0.000225 | 0.000725 |
Nucleus | 0.010406 | 0.007077 | 0.088579 | 0.246129* | 0.015761 | 0.007922 | 0.160319* | 0.151562* | 0.012734 | 0.007635 | 0.101615 | 0.198919* |
Peroxisomes | 0.000722 | 0.001860 | 0.000272 | 0.001073 | 0.000493 | 0.001795 | 0.000179 | 0.004097 | 0.000622 | 0.001817 | 0.000255 | 0.002583 |
Vacuole | 0.029266 | 0.045489 | 0.055697 | 0.150065 | 0.061541 | 0.073764 | 0.104721 | 0.151916 | 0.043298 | 0.064146 | 0.064605 | 0.150989 |
Vacuole Periphery | 0.005670 | 0.003059 | 0.000238 | 0.003505 | 0.004461 | 0.004084 | 0.000291 | 0.008791 | 0.005144 | 0.003735 | 0.000247 | 0.006144 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.64 | 4.42 | -4.78 | 1.40 | -6.48 | -1.92 | -6.24 | -2.69 | 0.43 | -2.12 | -2.40 | 1.58 | -3.70 | 1.44 | -3.91 |
Bud Neck | 0.46 | -1.38 | -5.77 | -5.98 | -4.56 | -2.77 | -1.68 | -7.06 | -5.43 | -6.64 | -1.63 | -2.24 | -8.99 | -7.66 | -6.87 |
Bud Site | -5.18 | 6.17 | 0.20 | 4.33 | -3.18 | -7.53 | 1.94 | -6.66 | -2.83 | -7.06 | -7.17 | 4.27 | -5.01 | 0.04 | -6.72 |
Cell Periphery | 0.25 | 11.14 | 0.63 | 0.08 | -8.15 | -3.62 | -2.87 | -3.01 | -0.04 | -2.05 | -1.64 | 9.69 | -1.88 | -0.00 | -6.41 |
Cytoplasm | 3.35 | -0.89 | 40.51 | 21.52 | 42.24* | 6.48 | 12.13 | 30.84 | 19.48 | 11.61 | 7.76 | 3.68 | 50.31 | 29.28 | 45.54* |
Cytoplasmic Foci | -1.00 | 36.82 | 35.99 | 16.66 | -0.29 | -5.41 | 26.65 | 27.24 | 24.75 | 0.73 | -4.65 | 46.33 | 44.72 | 30.12 | -1.49 |
Eisosomes | 2.29 | 10.81 | -13.13 | -10.93 | -25.17 | -4.21 | -9.33 | -21.74 | -5.40 | -6.41 | -1.85 | 5.49 | -22.55 | -9.45 | -29.53 |
Endoplasmic Reticulum | 4.87 | 6.01 | -3.11 | -11.15 | -13.27 | 0.54 | 2.99 | 3.14 | 1.89 | 0.50 | 1.51 | 6.98 | 0.42 | -1.44 | -14.08 |
Endosome | -3.24 | 13.81 | 4.57 | 4.86 | -5.55 | -6.24 | 9.09 | 1.17 | 6.41 | -4.73 | -7.76 | 17.01 | 2.79 | 8.74 | -7.67 |
Golgi | 7.55 | 25.63 | 14.29 | 3.67 | -1.69 | -1.78 | 17.52 | 0.13 | 1.28 | -3.78 | 4.40 | 29.52 | 8.93 | 3.28 | -4.12 |
Lipid Particles | -0.01 | 12.34 | -3.28 | -1.09 | -17.91 | -1.10 | 2.65 | -1.25 | -0.06 | -1.94 | -1.12 | 12.97 | -1.88 | -0.17 | -5.26 |
Mitochondria | 0.81 | 10.16 | 0.19 | -0.64 | -3.39 | -1.21 | 0.05 | -1.53 | 0.00 | -1.16 | -1.13 | 2.66 | -1.50 | 0.19 | -3.04 |
Mitotic Spindle | -2.85 | -6.25 | -6.40 | -1.52 | -3.41 | 0.02 | -0.54 | -7.29 | -7.10 | -6.17 | -2.60 | -4.32 | -9.76 | -6.76 | -7.24 |
None | 4.70 | -2.63 | -2.03 | -4.51 | -0.72 | 5.31 | -7.17 | -4.12 | -5.40 | -1.03 | 6.04 | -5.17 | -4.03 | -6.93 | -1.29 |
Nuclear Periphery | -2.26 | -23.40 | -20.58 | -19.99 | -17.29 | 0.72 | -14.27 | -8.24 | -8.32 | -3.97 | -1.10 | -24.52 | -18.07 | -17.79 | -14.06 |
Nuclear Periphery Foci | -3.56 | -5.23 | -10.79 | -1.08 | -7.48 | -5.44 | -9.12 | -3.19 | -0.07 | 5.94 | -6.42 | -10.07 | -8.03 | -1.15 | -1.17 |
Nucleolus | 0.29 | 1.12 | -1.19 | -1.04 | -4.67 | -0.53 | -1.13 | -4.14 | -3.42 | -3.74 | 0.10 | 0.88 | -3.95 | -3.46 | -5.19 |
Nucleus | 1.87 | -34.70 | -38.93 | -38.61 | -23.58 | 9.66 | -18.17 | -29.43 | -30.78 | -1.93 | 6.61 | -38.01 | -47.94 | -48.79 | -22.06 |
Peroxisomes | -5.64 | 6.48 | 1.56 | 5.61 | -1.57 | -2.95 | 6.01 | -2.91 | -0.21 | -3.52 | -4.01 | 6.51 | -2.29 | 1.54 | -3.73 |
Vacuole | -4.44 | -20.19 | -31.47 | -23.64 | -21.88 | -2.67 | -13.05 | -23.98 | -21.06 | -6.13 | -6.91 | -20.29 | -39.41 | -31.21 | -25.09 |
Vacuole Periphery | 1.82 | 9.85 | 6.30 | 1.14 | -2.54 | 0.38 | 9.40 | 0.69 | 0.21 | -4.06 | 1.68 | 13.32 | 4.29 | 1.23 | -4.76 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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