ORF
Human Ortholog
Description Nuclear protein of unknown function; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0.1 0.13 0.15 0.15 0.06 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.15 0.16 0.11 0.13 0.26 0.21 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.93 0.94 0.95 0.91 0.86 0.83 0.83 0.81 0.79 0.82 0.78 0.9 0.85 0.81 0.82 0.86 0.8 0.95 0.92 0.88 0.87 0.75 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.12 0.17 0.13 0.17 0.15 0.2 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0.07 0.16 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 0
Bud 0 0 0 0 0 1 4 4 1 2 2 1 0 2 0 0 0 0 0 1 0 0 6 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 1 0 0 0 4 21 19 10 10 17 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 2 9 11 10 17 20 15 16 16 7 10 33 82 100 17 5 11 0 0 1 4 3 4
Endoplasmic Reticulum 0 0 3 0 2 0 0 0 0 0 0 0 0 0 0 2 0 2 1 0 0 0 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 3 9 0 0 0 0 0 2 2 4 6
Golgi 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4 1 3 1
Mitochondria 15 4 0 1 14 49 76 59 56 140 85 101 1 0 3 1 1 2 0 1 3 1 3 6
Nucleus 185 92 296 189 308 281 404 449 344 418 332 333 293 554 538 94 67 57 181 103 277 312 505 388
Nuclear Periphery 0 1 2 0 3 4 14 16 17 5 3 6 5 19 10 0 0 0 0 0 0 0 2 3
Nucleolus 2 3 4 4 10 22 56 92 54 91 60 84 0 5 7 2 0 1 0 0 7 4 21 36
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 0 1 0 1 3 10 0 1 0 0 1 0 0 6 3
Vac/Vac Membrane 0 3 3 0 4 1 9 6 6 9 1 9 2 10 10 5 5 5 2 2 10 24 107 151
Unique Cell Count 191 99 315 200 337 328 486 543 425 532 407 425 327 651 667 114 78 71 190 112 316 359 673 613
Labelled Cell Count 205 106 320 205 351 379 605 661 505 691 508 563 336 678 697 121 79 78 190 112 316 359 673 613


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 8.9 8.2 7.6 7.2 6.3 5.9 6.0 6.0 5.7 5.6 5.5 9.9 9.8 9.0 11.3 11.0 11.0 9.6 9.9 10.3
Std Deviation (1e-4) 1.2 1.9 1.7 1.7 2.1 2.0 1.7 1.6 1.8 1.8 1.5 1.5 2.4 4.4 2.4 2.9 2.6 3.2 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.11 -0.19 -0.38 -0.47 -0.45 -0.44 -0.52 -0.54 -0.58 0.28 0.27 0.14 0.47 0.43 0.43 0.23 0.28 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.7 3.4 0 0 3.7 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 0.1 1.5 0.9 -0.1 0.7 0.1 -1.0 -0.4 3.7 4.9 5.7 4.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.7 7.1 7.4 6.1 6.7 10.0 8.6 9.3 0 0 0 0 0 0
Nucleus 0.3 -1.3 -3.5 -4.5 -4.7 -5.1 -6.0 -4.9 -5.9 -2.0 -4.0 -5.4 -3.7 -2.4 -3.7
Nuclear Periphery 0 0 0 2.2 2.3 2.9 0 0 0 0 2.3 0 0 0 0
Nucleolus 0 1.5 3.5 5.4 7.0 5.7 7.1 6.3 7.7 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 1.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3518 2.9023 2.6271 2.5067 1.8003 2.6537 4.6532 4.5999 4.4118 4.1735 3.8692 4.285 4.0467 4.0359 3.4478 3.9219 3.5832 3.5231
Actin 0.0002 0 0 0 0 0 0.015 0.0001 0.0026 0.0001 0.0118 0.0053 0.0051 0 0.0093 0.0046 0.0053 0.0062
Bud 0.0001 0 0 0 0 0 0.0005 0 0.0003 0.0002 0.0004 0.0001 0.0004 0 0.0003 0.0002 0.0003 0.0001
Bud Neck 0.0005 0.0001 0.0001 0.0002 0.0001 0.0006 0.0014 0.0001 0.0019 0.0007 0.0009 0.0011 0.0015 0 0.0018 0.0002 0.0006 0.0022
Bud Periphery 0.0003 0 0 0 0 0.0001 0.0016 0.0001 0.0007 0.0013 0.0014 0.0002 0.0013 0 0.0008 0.0005 0.0016 0.0002
Bud Site 0.0007 0 0 0 0 0 0.0012 0.0002 0.0032 0.0004 0.0006 0.0003 0.0014 0.0001 0.0022 0.0004 0.0004 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.001 0.0003 0 0.0006 0 0.0004 0 0.0004 0.0001
Cytoplasm 0.0006 0.0007 0.0001 0.0002 0 0.0001 0.0059 0.0003 0.0013 0.0003 0.0008 0.0003 0.004 0.0001 0.0019 0.0009 0.0011 0.0002
Cytoplasmic Foci 0.0005 0.0003 0.0001 0 0 0 0.0097 0.0027 0.0112 0.0001 0.0041 0.0024 0.0073 0 0.0113 0.0028 0.0025 0.0004
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0001 0 0 0.0001 0 0 0.0051 0 0.0009 0.001 0.0051 0.0003 0.0031 0 0.002 0.0004 0.0034 0.0005
Endosome 0.0005 0.0001 0.0001 0.0003 0 0 0.0174 0.0017 0.0123 0.0033 0.0202 0.0051 0.0151 0 0.0111 0.0034 0.0148 0.003
Golgi 0.0001 0 0 0 0 0 0.0045 0.0018 0.0033 0.0003 0.0143 0.0031 0.0024 0 0.0035 0.0016 0.003 0.0014
Lipid Particles 0.0003 0.0001 0.0002 0.0002 0 0 0.0159 0.0001 0.009 0.0003 0.0347 0.004 0.015 0 0.0185 0.0112 0.0078 0.0053
Mitochondria 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0081 0.0004 0.0147 0.001 0.051 0.0009 0.0072 0.0002 0.0041 0.0041 0.0233 0.0041
None 0.001 0.0053 0.0001 0.0001 0 0.0007 0.0027 0 0.0015 0 0.0006 0.0001 0.0026 0 0.0014 0.001 0.0009 0.0003
Nuclear Periphery 0.0029 0.0028 0.0057 0.0213 0.0019 0.0009 0.0211 0.002 0.0095 0.0012 0.0303 0.0009 0.0224 0.0007 0.0178 0.0095 0.0262 0.0056
Nucleolus 0.0366 0.0371 0.0387 0.029 0.0398 0.0655 0.0136 0.0151 0.0135 0.0098 0.0266 0.0512 0.0188 0.0138 0.022 0.0115 0.0322 0.049
Nucleus 0.9488 0.933 0.9519 0.9323 0.9566 0.9289 0.8535 0.9746 0.8973 0.9576 0.7787 0.9221 0.8699 0.9848 0.8708 0.9356 0.8521 0.9189
Peroxisomes 0.0001 0 0 0 0 0 0.0087 0.0003 0.0047 0 0.0012 0.0009 0.0035 0 0.0063 0.006 0.0021 0.0002
Punctate Nuclear 0.0049 0.0202 0.0024 0.0159 0.0015 0.0028 0.0055 0.0003 0.0062 0.0004 0.0103 0.0013 0.0092 0.0002 0.009 0.0047 0.0112 0.001
Vacuole 0.0007 0.0001 0.0001 0.0001 0 0.0002 0.0052 0.0001 0.0039 0.0188 0.0024 0.0002 0.0054 0.0001 0.004 0.0007 0.0038 0.0005
Vacuole Periphery 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0026 0.0002 0.0017 0.0022 0.0039 0.0001 0.0035 0 0.0015 0.0008 0.0066 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.0486 16.0181 18.1419 22.3257 20.7867 22.6214 19.3924 20.4239 20.5715 23.3146
Translational Efficiency 1.0162 1.2831 0.9849 0.9718 1.1525 1.1087 1.0604 0.8734 0.8952 0.9437

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1794 220 1494 1441 1942 911 1401 1424 3736 1131 2895 2865

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 896.72 1101.30 1131.82 1025.53 902.14 1049.43 1026.98 1112.31 899.54 1059.52 1081.08 1068.66
Standard Deviation 138.35 175.18 174.44 220.58 133.67 156.98 161.94 174.90 135.97 161.99 176.47 203.86
Intensity Change Log 2 — 0.296478 0.335915 0.193640 — 0.218183 0.186985 0.302136 — 0.257744 0.263147 0.249071

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000257 0.000757 0.000578 0.000713 0.000234 0.000450 0.000606 0.000813 0.000245 0.000510 0.000592 0.000763
Bud Neck 0.005501 0.012169 0.016588 0.022027 0.003363 0.010968 0.009967 0.033922 0.004389 0.011201 0.013384 0.027939
Bud Site 0.000568 0.003707 0.000835 0.002128 0.000392 0.001442 0.001073 0.004552 0.000476 0.001883 0.000950 0.003333
Cell Periphery 0.000406 0.000086 0.000058 0.000199 0.000208 0.000123 0.000135 0.000231 0.000303 0.000116 0.000095 0.000215
Cytoplasm 0.001107 0.000642 0.000251 0.005120 0.001092 0.000762 0.003303 0.006031 0.001099 0.000739 0.001728 0.005572
Cytoplasmic Foci 0.000082 0.001761 0.000011 0.000338 0.000244 0.002196 0.000327 0.000623 0.000166 0.002112 0.000164 0.000480
Eisosomes 0.000037 0.000035 0.000044 0.000028 0.000036 0.000032 0.000037 0.000027 0.000037 0.000032 0.000040 0.000028
Endoplasmic Reticulum 0.008051 0.004229 0.003146 0.005022 0.007591 0.005525 0.004359 0.004886 0.007812 0.005273 0.003733 0.004954
Endosome 0.000252 0.001471 0.000080 0.000853 0.000331 0.001588 0.000494 0.002502 0.000293 0.001565 0.000281 0.001673
Golgi 0.000039 0.001639 0.000032 0.000789 0.000251 0.001124 0.000159 0.002276 0.000149 0.001224 0.000093 0.001528
Lipid Particles 0.000091 0.000174 0.000029 0.000045 0.000151 0.000273 0.000098 0.000141 0.000122 0.000254 0.000062 0.000093
Mitochondria 0.000887 0.001216 0.001109 0.001894 0.000549 0.001587 0.001095 0.005061 0.000711 0.001515 0.001102 0.003468
Mitotic Spindle 0.000747 0.007028 0.000339 0.004257 0.000441 0.005778 0.001084 0.018902 0.000588 0.006021 0.000699 0.011536
None 0.001537 0.001256 0.000677 0.004373 0.001339 0.001523 0.006774 0.002940 0.001434 0.001471 0.003628 0.003660
Nuclear Periphery 0.000517 0.000258 0.000218 0.001469 0.000422 0.000383 0.000308 0.002966 0.000468 0.000359 0.000262 0.002213
Nuclear Periphery Foci 0.000166 0.000106 0.000034 0.000508 0.000333 0.000094 0.000125 0.001333 0.000253 0.000097 0.000078 0.000918
Nucleolus 0.007319 0.009487 0.006981 0.007608 0.007362 0.008276 0.008482 0.008196 0.007341 0.008511 0.007707 0.007900
Nucleus 0.970543* 0.950793* 0.968172* 0.938163* 0.974593* 0.955251* 0.959458* 0.898015* 0.972649* 0.954384* 0.963955* 0.918208*
Peroxisomes 0.000112 0.000910 0.000213 0.000143 0.000125 0.000567 0.000191 0.000179 0.000119 0.000634 0.000202 0.000161
Vacuole 0.001410 0.001769 0.000506 0.003010 0.000749 0.001420 0.001103 0.004619 0.001066 0.001488 0.000795 0.003810
Vacuole Periphery 0.000371 0.000507 0.000099 0.001315 0.000192 0.000637 0.000821 0.001784 0.000278 0.000612 0.000449 0.001548

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.30 -8.52 -11.67 -0.05 -3.57 -5.25 -13.12 -8.98 -6.34 -4.24 -5.04 -14.76 -13.24 -5.70 -5.45
Bud Neck -2.34 -11.88 -12.16 -4.07 -4.98 -8.38 -11.64 -18.53 -13.23 -13.84 -7.37 -16.62 -21.95 -13.57 -13.61
Bud Site -2.22 -2.56 -5.44 0.89 -4.43 -2.61 -4.52 -6.72 -4.08 -5.26 -3.32 -5.19 -8.52 -2.74 -6.75
Cell Periphery 1.58 1.74 1.10 -1.57 -2.27 1.52 1.54 0.48 -0.52 -0.46 1.83 2.11 1.20 -1.07 -1.69
Cytoplasm 0.77 1.69 -3.53 -4.23 -4.72 0.65 -3.61 -4.20 -4.48 -1.89 0.80 -1.65 -5.45 -6.04 -4.64
Cytoplasmic Foci -1.32 1.36 -1.62 1.15 -2.46 -2.18 -0.25 -0.61 2.06 -0.41 -2.58 0.18 -1.33 2.36 -1.73
Eisosomes 0.30 -1.90 2.17 1.66 9.11 0.54 -0.31 0.67 0.25 4.06 0.82 -1.20 1.59 1.12 9.41
Endoplasmic Reticulum 6.36 10.19 5.65 -1.33 -5.17 4.04 7.58 4.67 0.21 -2.91 6.13 12.83 7.35 0.39 -5.86
Endosome -1.52 4.43 -2.28 0.85 -3.06 -2.10 -0.71 -3.26 0.01 -2.70 -2.51 0.24 -3.95 0.73 -3.84
Golgi -1.17 0.39 -1.88 0.70 -1.89 -1.60 0.42 -1.81 -0.33 -2.10 -2.18 0.48 -2.46 0.36 -2.78
Lipid Particles -0.63 1.56 0.93 0.93 -2.65 -0.72 0.41 -0.32 0.63 -2.43 -1.02 0.97 0.05 1.13 -3.18
Mitochondria -0.98 -1.14 -4.11 -2.59 -5.86 -2.58 -3.06 -4.80 -3.12 -3.96 -2.36 -2.86 -5.97 -3.27 -4.98
Mitotic Spindle -1.58 0.83 -3.19 0.81 -3.57 -3.01 -2.06 -6.13 -3.17 -5.30 -3.32 -1.41 -6.90 -1.59 -6.34
None 0.50 2.84 -2.88 -3.03 -4.03 -0.41 -4.54 -4.28 -2.53 2.94 -0.15 -3.54 -4.34 -3.45 -0.29
Nuclear Periphery 1.63 2.12 -3.57 -4.67 -4.97 -0.12 0.25 -3.96 -3.97 -4.05 0.70 1.90 -5.16 -5.44 -5.77
Nuclear Periphery Foci 0.67 1.99 -2.52 -2.87 -3.49 1.62 1.29 -1.75 -2.41 -2.30 1.76 2.03 -2.56 -3.36 -3.46
Nucleolus -1.49 -0.17 -2.71 -0.63 -2.49 -1.26 -1.97 -5.53 -4.02 -4.00 -1.69 -1.66 -6.06 -3.46 -4.73
Nucleus 2.21 1.99 9.86 1.66 9.24 4.80 6.58 16.51 9.91 12.05 5.03 6.49 18.96 8.55 15.16
Peroxisomes -1.52 -4.00 -2.58 1.42 2.06 -1.45 -2.99 -3.89 1.03 -1.73 -1.95 -4.90 -4.61 1.60 0.27
Vacuole -0.57 1.94 -2.79 -1.90 -4.01 -2.26 -2.51 -7.31 -4.94 -5.58 -1.70 -0.04 -6.56 -4.65 -6.71
Vacuole Periphery -0.49 1.60 -1.90 -1.42 -2.88 -2.34 -1.26 -3.21 -1.40 -0.82 -1.90 -0.57 -3.49 -1.79 -2.35
ORF
Human Ortholog
Description Nuclear protein of unknown function; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

YNL035C

YNL035C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl035c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available