Standard name | |
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Human Ortholog | |
Description | Protein with a role in snRNA and snoRNA cap trimethylation; interacts with Tgs1p and shows similar phenotypes; required for trimethylation of the caps of spliceosomal snRNAs and the U3 snoRNA, and for efficient 3' end processing of U3 snoRNA; may act as a specificity factor for Tgs1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 |
Bud | 1 | 0 | 11 | 4 | 2 | 6 | – | 10 | 15 | 25 | 15 | 30 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 1 | 3 | 3 | 2 | 5 |
Bud Neck | 0 | 0 | 3 | 0 | 2 | 0 | – | 1 | 1 | 3 | 2 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 8 | 2 | 10 |
Bud Site | 3 | 0 | 2 | 21 | 6 | 8 | – | 25 | 33 | 39 | 23 | 42 | 0 | 1 | 0 | 3 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 8 | 16 | 14 | 18 | 18 | – | 34 | 27 | 36 | 39 | 26 | 37 | 94 | 127 | 36 | 24 | 21 | 1 | 2 | 2 | 2 | 3 | 6 |
Endoplasmic Reticulum | 0 | 1 | 0 | 4 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 6 | 4 | 4 | 0 | 0 | 1 | 1 | 1 | 1 |
Endosome | 13 | 5 | 4 | 14 | 69 | 34 | – | 31 | 24 | 49 | 7 | 15 | 56 | 110 | 144 | 48 | 17 | 18 | 17 | 7 | 40 | 39 | 48 | 72 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 6 |
Mitochondria | 109 | 19 | 197 | 94 | 48 | 148 | – | 239 | 328 | 322 | 278 | 363 | 11 | 7 | 4 | 32 | 10 | 18 | 6 | 2 | 5 | 6 | 7 | 6 |
Nucleus | 215 | 69 | 159 | 64 | 43 | 45 | – | 76 | 78 | 77 | 54 | 61 | 137 | 145 | 127 | 86 | 39 | 54 | 111 | 39 | 56 | 39 | 38 | 36 |
Nuclear Periphery | 0 | 0 | 4 | 1 | 0 | 1 | – | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 4 | 2 | 2 | 3 |
Nucleolus | 126 | 65 | 130 | 212 | 141 | 142 | – | 195 | 174 | 180 | 98 | 141 | 237 | 291 | 315 | 113 | 50 | 68 | 185 | 72 | 156 | 128 | 119 | 153 |
Peroxisomes | 0 | 0 | 0 | 13 | 21 | 7 | – | 15 | 4 | 8 | 3 | 6 | 4 | 8 | 18 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 34 | 17 | 50 | 118 | 111 | 105 | – | 175 | 121 | 180 | 85 | 129 | 120 | 141 | 210 | 106 | 37 | 72 | 54 | 22 | 76 | 101 | 122 | 130 |
Vac/Vac Membrane | 7 | 4 | 1 | 2 | 9 | 7 | – | 2 | 11 | 6 | 6 | 13 | 7 | 5 | 7 | 20 | 2 | 7 | 18 | 5 | 17 | 24 | 18 | 34 |
Unique Cell Count | 353 | 148 | 349 | 321 | 293 | 323 | 506 | 506 | 577 | 396 | 537 | 417 | 550 | 630 | 295 | 126 | 177 | 405 | 162 | 382 | 370 | 381 | 479 | |
Labelled Cell Count | 513 | 188 | 577 | 561 | 470 | 521 | 804 | 817 | 926 | 611 | 832 | 613 | 803 | 953 | 456 | 183 | 265 | 405 | 162 | 382 | 370 | 381 | 479 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.5 | 4.3 | 4.3 | 4.8 | 3.6 | – | 3.8 | 3.2 | 3.5 | 3.2 | 3.2 | 5.5 | 5.9 | 5.7 | 6.6 | 6.7 | 6.6 | 5.7 | 6.0 | 6.2 |
Std Deviation (1e-4) | 0.6 | 1.1 | 1.4 | 1.6 | 1.9 | 1.2 | – | 1.4 | 1.1 | 1.4 | 0.9 | 1.1 | 1.2 | 1.7 | 1.8 | 1.6 | 1.6 | 1.7 | 1.6 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.14 | -0.24 | – | -0.18 | -0.42 | -0.29 | -0.42 | -0.42 | 0.35 | 0.45 | 0.41 | 0.62 | 0.65 | 0.62 | 0.42 | 0.49 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.7 | 0 | 0 | -2.2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | 0.9 | 0.6 | – | 1.3 | 0.5 | 1.1 | 2.7 | 0.2 | 2.3 | 5.6 | 6.6 | 3.5 | 5.0 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6.3 | 8.3 | 9.1 | 7.0 | 5.8 | 4.9 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -16.7 | -19.2 | -20.7 | -12.0 | -9.4 | -10.2 |
Nucleus | -7.0 | -8.4 | -8.9 | – | -9.8 | -9.7 | -10.9 | -9.6 | -11.5 | -3.6 | -5.9 | -8.4 | -4.3 | -2.9 | -3.3 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.4 | 4.6 | 3.8 | 0.3 | 0.5 | 0.3 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.8 | 4.0 | 6.4 | 6.4 | 3.7 | 6.8 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 4.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8057 | 1.5123 | 1.6186 | 1.6207 | 1.5386 | 1.388 | 0.7559 | 0.589 | 0.6098 | 0.3636 | 1.0852 | 0.742 | 2.2283 | 2.3346 | 1.9305 | 1.9897 | 1.8049 | 2.218 |
Actin | 0.0104 | 0 | 0.0009 | 0.0026 | 0.0179 | 0.0006 | 0.026 | 0.0007 | 0.0112 | 0 | 0.0032 | 0.0013 | 0.0148 | 0 | 0.0003 | 0.0001 | 0.0482 | 0.0017 |
Bud | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0032 | 0.0021 | 0.0019 | 0.0053 | 0.0062 | 0.0027 | 0.0039 | 0.0022 | 0.0028 | 0.0019 | 0.0024 | 0.0028 | 0.004 | 0.0073 | 0.0026 | 0.0028 | 0.003 | 0.0034 |
Bud Periphery | 0.0012 | 0.0002 | 0.0003 | 0.0015 | 0.0009 | 0.0003 | 0.0019 | 0.0003 | 0.0008 | 0.0002 | 0.0004 | 0.0004 | 0.001 | 0.0001 | 0.0004 | 0.0008 | 0.0004 | 0.0002 |
Bud Site | 0.0072 | 0.0014 | 0.0008 | 0.0027 | 0.0055 | 0.0003 | 0.0029 | 0.002 | 0.0046 | 0.0011 | 0.0012 | 0.0005 | 0.0033 | 0.0015 | 0.0008 | 0.0044 | 0.0007 | 0.0007 |
Cell Periphery | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Cytoplasm | 0.003 | 0.0001 | 0.0003 | 0.0012 | 0.0085 | 0.0001 | 0.0036 | 0.0008 | 0.0008 | 0.0001 | 0.0005 | 0.0035 | 0.005 | 0.0021 | 0.0002 | 0.0001 | 0.0002 | 0.0004 |
Cytoplasmic Foci | 0.0145 | 0.0004 | 0.0026 | 0.0081 | 0.0176 | 0.0029 | 0.0345 | 0.0095 | 0.0034 | 0.0004 | 0.0355 | 0.0027 | 0.0104 | 0.0016 | 0.0014 | 0.0012 | 0.0014 | 0.0015 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0 | 0.0002 | 0.001 | 0.0025 | 0.0001 | 0.0044 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0005 | 0.0026 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0006 |
Endosome | 0.0316 | 0.0003 | 0.0065 | 0.0299 | 0.0368 | 0.0037 | 0.0361 | 0.0043 | 0.0042 | 0.0002 | 0.0084 | 0.0056 | 0.0167 | 0.0017 | 0.0014 | 0.0006 | 0.0025 | 0.0047 |
Golgi | 0.0086 | 0.0001 | 0.0042 | 0.0059 | 0.0159 | 0.0026 | 0.0187 | 0.0018 | 0.0042 | 0.0001 | 0.0058 | 0.0102 | 0.0047 | 0.0002 | 0.0003 | 0.0003 | 0.0027 | 0.0017 |
Lipid Particles | 0.0361 | 0.0019 | 0.0143 | 0.0121 | 0.0373 | 0.0117 | 0.072 | 0.0097 | 0.0174 | 0.0011 | 0.0156 | 0.0144 | 0.0157 | 0.0073 | 0.007 | 0.0017 | 0.0042 | 0.0062 |
Mitochondria | 0.0123 | 0.0001 | 0.0072 | 0.0062 | 0.0351 | 0.0029 | 0.0164 | 0.0007 | 0.0277 | 0.0002 | 0.0008 | 0.0133 | 0.0027 | 0.0002 | 0.0007 | 0.001 | 0.0006 | 0.0009 |
None | 0.0017 | 0.0001 | 0.0002 | 0.0003 | 0.0027 | 0.0002 | 0.0056 | 0.0011 | 0.0009 | 0.0002 | 0.0006 | 0.0006 | 0.0065 | 0.0007 | 0.0002 | 0.0003 | 0.0009 | 0.0006 |
Nuclear Periphery | 0.0158 | 0.0002 | 0.0062 | 0.0029 | 0.0056 | 0.0022 | 0.0224 | 0.0025 | 0.0021 | 0.0001 | 0.0012 | 0.0062 | 0.0079 | 0.0017 | 0.0026 | 0.0002 | 0.0014 | 0.0041 |
Nucleolus | 0.535 | 0.6837 | 0.7587 | 0.6101 | 0.4329 | 0.6948 | 0.45 | 0.6979 | 0.6754 | 0.7205 | 0.548 | 0.6472 | 0.6148 | 0.6371 | 0.7297 | 0.8084 | 0.5755 | 0.6552 |
Nucleus | 0.2059 | 0.277 | 0.1577 | 0.2105 | 0.2656 | 0.1978 | 0.1822 | 0.2181 | 0.199 | 0.2359 | 0.3099 | 0.2084 | 0.1914 | 0.285 | 0.2157 | 0.1244 | 0.2204 | 0.2068 |
Peroxisomes | 0.0083 | 0.0001 | 0.0015 | 0.0014 | 0.0141 | 0.0026 | 0.0229 | 0.0041 | 0.0016 | 0.0001 | 0.0379 | 0.0012 | 0.0099 | 0.0002 | 0.0006 | 0.0003 | 0.0012 | 0.0006 |
Punctate Nuclear | 0.0906 | 0.0314 | 0.0307 | 0.0904 | 0.09 | 0.0735 | 0.0767 | 0.0371 | 0.0367 | 0.0365 | 0.0274 | 0.0787 | 0.0844 | 0.0512 | 0.0338 | 0.0503 | 0.1351 | 0.1092 |
Vacuole | 0.0072 | 0.0006 | 0.0044 | 0.0053 | 0.0027 | 0.0005 | 0.0124 | 0.006 | 0.005 | 0.001 | 0.0006 | 0.0009 | 0.0024 | 0.0013 | 0.0015 | 0.0019 | 0.0004 | 0.0008 |
Vacuole Periphery | 0.0047 | 0.0001 | 0.0011 | 0.0016 | 0.0011 | 0.0003 | 0.0053 | 0.0006 | 0.0008 | 0.0001 | 0.0002 | 0.0012 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.2551 | 6.71 | 6.6389 | 7.3056 | 1.622 | 2.5007 | 6.2191 | 8.395 | 8.7642 | 5.0548 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1711 | 1755 | 382 | 708 | 666 | 1868 | 772 | 1131 | 2377 | 3623 | 1154 | 1839 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 699.58 | 756.65 | 868.49 | 849.04 | 534.90 | 772.54 | 834.42 | 783.94 | 653.44 | 764.84 | 845.70 | 809.00 |
Standard Deviation | 80.09 | 104.05 | 110.22 | 125.40 | 82.26 | 104.28 | 130.36 | 119.95 | 109.46 | 104.47 | 125.09 | 126.12 |
Intensity Change Log 2 | — | 0.113137 | 0.312020 | 0.279343 | — | 0.530340 | 0.641505 | 0.551474 | — | 0.308864 | 0.464099 | 0.403604 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000113 | 0.000408 | 0.000505 | 0.001078 | 0.000248 | 0.000279 | 0.000545 | 0.001394 | 0.000151 | 0.000341 | 0.000532 | 0.001272 |
Bud Neck | 0.005219 | 0.010515 | 0.006008 | 0.030825 | 0.004672 | 0.007793 | 0.017533 | 0.027579 | 0.005066 | 0.009111 | 0.013718 | 0.028829 |
Bud Site | 0.000597 | 0.001497 | 0.000981 | 0.012744 | 0.003448 | 0.001649 | 0.002218 | 0.021226 | 0.001396 | 0.001576 | 0.001809 | 0.017961 |
Cell Periphery | 0.000065 | 0.000086 | 0.000064 | 0.000144 | 0.000237 | 0.000073 | 0.000064 | 0.000501 | 0.000113 | 0.000079 | 0.000064 | 0.000364 |
Cytoplasm | 0.018720 | 0.010886 | 0.011918 | 0.031490 | 0.039674 | 0.011035 | 0.003319 | 0.089188 | 0.024591 | 0.010963 | 0.006166 | 0.066975 |
Cytoplasmic Foci | 0.007087 | 0.005928 | 0.002248 | 0.006443 | 0.036650 | 0.009757 | 0.000300 | 0.012557 | 0.015370 | 0.007902 | 0.000945 | 0.010203 |
Eisosomes | 0.000051 | 0.000063 | 0.000066 | 0.000080 | 0.000153 | 0.000065 | 0.000041 | 0.000075 | 0.000080 | 0.000064 | 0.000049 | 0.000077 |
Endoplasmic Reticulum | 0.000872 | 0.002234 | 0.002049 | 0.004865 | 0.001429 | 0.002144 | 0.002123 | 0.003709 | 0.001028 | 0.002188 | 0.002099 | 0.004154 |
Endosome | 0.002646 | 0.002486 | 0.000590 | 0.008492 | 0.002422 | 0.003453 | 0.001181 | 0.022827 | 0.002583 | 0.002985 | 0.000986 | 0.017308 |
Golgi | 0.001023 | 0.001493 | 0.000328 | 0.003782 | 0.002995 | 0.002703 | 0.000490 | 0.005380 | 0.001576 | 0.002116 | 0.000436 | 0.004765 |
Lipid Particles | 0.003097 | 0.003474 | 0.000861 | 0.001553 | 0.011860 | 0.004947 | 0.000185 | 0.004134 | 0.005552 | 0.004233 | 0.000409 | 0.003140 |
Mitochondria | 0.003168 | 0.008672 | 0.000768 | 0.009493 | 0.008255 | 0.012331 | 0.001175 | 0.016216 | 0.004593 | 0.010559 | 0.001040 | 0.013628 |
Mitotic Spindle | 0.002930 | 0.018987 | 0.006687 | 0.060805 | 0.006186 | 0.019418 | 0.013695 | 0.073123 | 0.003843 | 0.019210 | 0.011375 | 0.068381 |
None | 0.014707 | 0.010475 | 0.009584 | 0.011066 | 0.076315 | 0.010020 | 0.003244 | 0.029473 | 0.031968 | 0.010241 | 0.005342 | 0.022387 |
Nuclear Periphery | 0.001099 | 0.001985 | 0.000489 | 0.002124 | 0.000794 | 0.003076 | 0.000589 | 0.002183 | 0.001014 | 0.002548 | 0.000555 | 0.002161 |
Nuclear Periphery Foci | 0.006798 | 0.005937 | 0.003897 | 0.006596 | 0.007827 | 0.004597 | 0.002974 | 0.003950 | 0.007086 | 0.005246 | 0.003279 | 0.004969 |
Nucleolus | 0.543908* | 0.441418* | 0.327068* | 0.332247* | 0.452873* | 0.407769* | 0.456946* | 0.151797 | 0.518402* | 0.424069* | 0.413953* | 0.221269* |
Nucleus | 0.384531* | 0.467060* | 0.622739* | 0.448045* | 0.334643* | 0.490364* | 0.490072* | 0.489267* | 0.370553* | 0.479075* | 0.533988* | 0.473397* |
Peroxisomes | 0.001752 | 0.003742 | 0.001784 | 0.003610 | 0.006931 | 0.004852 | 0.001285 | 0.004383 | 0.003203 | 0.004315 | 0.001450 | 0.004086 |
Vacuole | 0.001250 | 0.001847 | 0.001120 | 0.020058 | 0.001413 | 0.002643 | 0.001477 | 0.031959 | 0.001296 | 0.002257 | 0.001359 | 0.027377 |
Vacuole Periphery | 0.000368 | 0.000804 | 0.000247 | 0.004459 | 0.000974 | 0.001033 | 0.000544 | 0.009078 | 0.000537 | 0.000922 | 0.000446 | 0.007300 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.26 | -8.85 | -13.10 | -9.38 | -5.91 | -0.54 | -6.82 | -8.09 | -8.22 | -5.02 | -8.12 | -13.87 | -13.74 | -11.33 | -7.24 |
Bud Neck | -7.77 | -3.72 | -15.61 | -13.01 | -13.54 | -2.76 | -9.73 | -14.94 | -14.33 | -5.00 | -7.47 | -10.85 | -21.92 | -19.09 | -10.49 |
Bud Site | -3.99 | -2.27 | -8.02 | -7.43 | -6.77 | 1.69 | 0.11 | -6.90 | -8.42 | -7.66 | -0.40 | -3.48 | -11.00 | -10.93 | -9.93 |
Cell Periphery | -2.06 | 0.12 | -5.12 | -3.15 | -4.66 | 6.93 | 6.40 | -0.96 | -2.86 | -2.92 | 3.45 | 3.86 | -2.57 | -3.19 | -3.43 |
Cytoplasm | 3.45 | 2.85 | -3.13 | -5.26 | -4.85 | 5.80 | 8.04 | -6.35 | -13.01 | -15.00 | 6.25 | 8.99 | -9.38 | -13.87 | -15.44 |
Cytoplasmic Foci | 1.45 | 5.78 | 3.52 | 2.43 | -2.02 | 7.40 | 10.20 | 8.30 | 2.32 | -7.42 | 5.87 | 12.05 | 7.72 | 2.85 | -7.57 |
Eisosomes | -4.69 | -3.64 | -8.11 | -5.45 | -3.20 | 3.45 | 4.37 | 2.73 | -4.32 | -10.08 | 1.93 | 3.59 | -1.08 | -6.91 | -9.68 |
Endoplasmic Reticulum | -9.59 | -8.41 | -15.17 | -10.63 | -9.45 | -3.59 | -5.51 | -13.50 | -9.98 | -7.99 | -9.85 | -10.84 | -22.26 | -14.62 | -12.15 |
Endosome | 0.08 | 4.93 | -3.88 | -4.16 | -6.73 | -2.25 | 1.94 | -5.80 | -5.07 | -6.43 | -1.38 | 3.94 | -6.57 | -6.18 | -8.05 |
Golgi | -1.51 | 2.16 | -1.44 | -0.73 | -2.45 | 0.43 | 3.44 | 0.30 | -0.15 | -4.30 | -1.37 | 3.80 | -1.83 | -0.76 | -4.87 |
Lipid Particles | -0.82 | 6.39 | 4.20 | 4.39 | -2.08 | 4.30 | 7.80 | 5.96 | 3.50 | -4.03 | 2.06 | 10.46 | 5.93 | 4.76 | -4.57 |
Mitochondria | -4.86 | 3.78 | -1.47 | 1.89 | -3.19 | -2.64 | 6.39 | -0.35 | 1.87 | -5.01 | -6.52 | 6.34 | -2.51 | 2.30 | -5.91 |
Mitotic Spindle | -7.43 | -2.84 | -11.42 | -8.80 | -9.33 | -3.52 | -3.48 | -11.68 | -9.91 | -9.11 | -8.61 | -5.66 | -17.06 | -13.24 | -12.79 |
None | 3.04 | 2.25 | 3.77 | 1.43 | 0.27 | 10.38 | 11.62 | 8.75 | -3.50 | -7.11 | 10.20 | 12.36 | 7.18 | -2.66 | -5.81 |
Nuclear Periphery | -3.38 | 2.92 | -7.58 | -3.72 | -10.80 | -4.60 | -1.17 | -10.18 | 0.87 | -9.00 | -5.18 | 1.41 | -10.63 | -0.85 | -13.42 |
Nuclear Periphery Foci | 0.70 | 2.05 | 0.29 | -0.26 | -1.62 | 2.66 | 3.17 | 3.95 | 2.21 | 0.48 | 2.33 | 3.74 | 3.31 | 1.59 | -0.88 |
Nucleolus | 10.68 | 12.59 | 20.68 | 12.92 | 3.60 | 4.05 | 0.94 | 21.33 | 25.92* | 24.27* | 12.69 | 10.62 | 35.81* | 28.49* | 20.56 |
Nucleus | -8.90 | -13.47 | -5.63 | 0.91 | 7.95 | -14.20 | -11.87 | -14.08 | -2.19 | -2.42 | -15.65 | -15.96 | -13.90 | -1.59 | 2.72 |
Peroxisomes | -3.90 | -0.53 | -1.62 | 2.00 | -1.26 | 2.87 | 7.01 | 4.62 | 2.37 | -1.95 | -1.36 | 5.84 | 1.74 | 2.83 | -2.36 |
Vacuole | -1.86 | -0.82 | -8.52 | -8.31 | -8.45 | -2.16 | -2.15 | -12.27 | -11.87 | -11.72 | -3.09 | -2.65 | -14.98 | -14.51 | -14.39 |
Vacuole Periphery | -3.64 | 1.04 | -3.37 | -2.61 | -3.48 | -0.40 | 0.52 | -3.93 | -4.15 | -4.66 | -2.75 | -0.10 | -5.54 | -4.89 | -5.63 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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