Standard name | |
---|---|
Human Ortholog | |
Description | Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | – | 6 | 8 | 11 | 5 | 13 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 46 | 64 | 66 | 52 | 103 | 236 | 321 | 474 | 229 | 358 | 217 | 307 | 81 | 132 | 201 | 95 | 71 | – | 39 | 48 | 60 | 22 | 52 | 67 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 6 | 5 | – | 1 | 1 | 1 | 1 | 2 | 4 |
Endosome | 0 | 0 | 1 | 3 | 3 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 7 | 3 | 2 | – | 4 | 1 | 5 | 0 | 6 | 8 |
Golgi | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 21 | 10 | 8 | 14 | 13 | 123 | 178 | 258 | 177 | 279 | 235 | 310 | 2 | 2 | 1 | 10 | 5 | – | 0 | 1 | 1 | 2 | 1 | 1 |
Nucleus | 34 | 48 | 45 | 51 | 46 | 24 | 14 | 34 | 22 | 22 | 16 | 15 | 66 | 53 | 45 | 46 | 23 | – | 78 | 146 | 96 | 63 | 138 | 107 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 2 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 4 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 5 | 3 | 12 | 7 |
Vac/Vac Membrane | 59 | 152 | 86 | 68 | 81 | 56 | 77 | 77 | 19 | 38 | 23 | 32 | 108 | 197 | 203 | 66 | 41 | – | 19 | 29 | 39 | 10 | 26 | 66 |
Unique Cell Count | 133 | 216 | 181 | 167 | 218 | 373 | 490 | 725 | 356 | 559 | 398 | 517 | 231 | 331 | 395 | 189 | 118 | 157 | 243 | 232 | 115 | 265 | 302 | |
Labelled Cell Count | 161 | 274 | 209 | 188 | 250 | 445 | 597 | 854 | 457 | 710 | 500 | 673 | 260 | 386 | 457 | 228 | 151 | 157 | 243 | 232 | 115 | 265 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.1 | 5.4 | 5.8 | 5.4 | 4.5 | 3.8 | 4.0 | 3.7 | 3.6 | 3.6 | 3.6 | 7.1 | 6.5 | 6.1 | 6.7 | 7.6 | – | 6.8 | 6.8 | 7.2 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.8 | 1.2 | 1.1 | 1.0 | 0.7 | 0.8 | 0.6 | 1.0 | 0.6 | 1.0 | 1.3 | 1.2 | 1.2 | 1.1 | 1.5 | – | 1.3 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.11 | -0.0 | -0.28 | -0.51 | -0.42 | -0.54 | -0.61 | -0.61 | -0.61 | 0.4 | 0.27 | 0.18 | 0.31 | 0.48 | – | 0.33 | 0.33 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | -1.0 | 2.2 | 5.9 | 6.8 | 7.1 | 6.1 | 6.5 | 4.0 | 5.3 | -0.3 | 0.8 | 3.2 | 2.7 | 4.0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | 1.5 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | -0.1 | – |
Nucleus | 1.2 | -0.9 | -6.2 | -8.9 | -8.6 | -6.2 | -8.5 | -7.6 | -9.1 | 0.8 | -2.4 | -4.1 | -0.1 | -1.1 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -1.3 | -2.1 | -8.2 | -8.5 | -11.6 | -11.6 | -12.7 | -11.9 | -12.8 | -0.2 | 2.6 | 0.9 | -2.5 | -2.2 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.571 | 1.6187 | 1.3748 | 1.0985 | 1.1314 | 1.454 | 0.1857 | 0.7058 | 0.4014 | 0.3696 | 0.5679 | 0.5285 | 1.2566 | 1.703 | 1.1667 | 1.6098 | 0.7501 | 1.2967 |
Actin | 0.0259 | 0.0005 | 0.0125 | 0.0104 | 0.0026 | 0.0006 | 0.0342 | 0.0011 | 0.0032 | 0.0152 | 0.0139 | 0.0023 | 0.0194 | 0.0003 | 0.0296 | 0.0015 | 0.0032 | 0.0003 |
Bud | 0.0056 | 0.0026 | 0.0006 | 0.0003 | 0.0008 | 0.0002 | 0.0023 | 0.0011 | 0.0003 | 0.0009 | 0.0003 | 0.0003 | 0.002 | 0.0001 | 0.0061 | 0.0003 | 0.0002 | 0.0007 |
Bud Neck | 0.0024 | 0.0007 | 0.0007 | 0.0041 | 0.0009 | 0.0036 | 0.0036 | 0.0006 | 0.0004 | 0.0073 | 0.0023 | 0.0026 | 0.0259 | 0.0002 | 0.0007 | 0.0011 | 0.0007 | 0.0011 |
Bud Periphery | 0.0033 | 0.0008 | 0.0023 | 0.0004 | 0.0021 | 0.0002 | 0.0036 | 0.0008 | 0.0004 | 0.0013 | 0.0003 | 0.0009 | 0.0039 | 0 | 0.0065 | 0.0002 | 0.0003 | 0.0002 |
Bud Site | 0.0142 | 0.0148 | 0.0018 | 0.0017 | 0.0015 | 0.0003 | 0.0131 | 0.0244 | 0.001 | 0.0077 | 0.0024 | 0.0004 | 0.052 | 0.0008 | 0.0073 | 0.0103 | 0.0003 | 0.0002 |
Cell Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0024 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.2505 | 0.4485 | 0.3025 | 0.3568 | 0.3286 | 0.3238 | 0.2388 | 0.356 | 0.27 | 0.1511 | 0.3643 | 0.2979 | 0.392 | 0.3193 | 0.3114 | 0.2971 | 0.3607 | 0.44 |
Cytoplasmic Foci | 0.033 | 0.0125 | 0.0105 | 0.0099 | 0.018 | 0.0066 | 0.0351 | 0.0094 | 0.0068 | 0.0225 | 0.0413 | 0.0093 | 0.0103 | 0.0025 | 0.0145 | 0.0297 | 0.0114 | 0.0119 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0019 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.009 | 0.0043 | 0.0056 | 0.0042 | 0.0049 | 0.0056 | 0.0117 | 0.0056 | 0.0045 | 0.0032 | 0.0088 | 0.003 | 0.009 | 0.007 | 0.0041 | 0.0024 | 0.0071 | 0.0037 |
Endosome | 0.0333 | 0.0028 | 0.0121 | 0.0053 | 0.0089 | 0.0087 | 0.027 | 0.0028 | 0.0041 | 0.0385 | 0.0231 | 0.004 | 0.0137 | 0.0032 | 0.0049 | 0.004 | 0.0256 | 0.0094 |
Golgi | 0.0132 | 0.0003 | 0.0033 | 0.0054 | 0.0006 | 0.0002 | 0.0109 | 0.0003 | 0.0014 | 0.0208 | 0.0084 | 0.0008 | 0.0044 | 0.0001 | 0.0067 | 0.004 | 0.0274 | 0.0011 |
Lipid Particles | 0.0397 | 0.0008 | 0.0042 | 0.002 | 0.0003 | 0.0004 | 0.0273 | 0.0003 | 0.0003 | 0.009 | 0.0131 | 0.0035 | 0.0045 | 0.0001 | 0.0025 | 0.0075 | 0.0011 | 0.0046 |
Mitochondria | 0.0084 | 0.0007 | 0.007 | 0.0113 | 0.0536 | 0.0012 | 0.0078 | 0.0005 | 0.0109 | 0.0057 | 0.0012 | 0.0064 | 0.026 | 0.0003 | 0.0033 | 0.0034 | 0.0367 | 0.0011 |
None | 0.2516 | 0.2666 | 0.3393 | 0.2115 | 0.2873 | 0.2288 | 0.3591 | 0.3601 | 0.3688 | 0.4473 | 0.1401 | 0.4044 | 0.2938 | 0.2671 | 0.2773 | 0.3034 | 0.3049 | 0.2535 |
Nuclear Periphery | 0.016 | 0.0056 | 0.0105 | 0.0116 | 0.0101 | 0.0111 | 0.0164 | 0.012 | 0.0123 | 0.0052 | 0.0122 | 0.0045 | 0.0113 | 0.0228 | 0.0124 | 0.0054 | 0.0135 | 0.0049 |
Nucleolus | 0.0041 | 0.0013 | 0.0021 | 0.0021 | 0.0012 | 0.0024 | 0.0028 | 0.001 | 0.0011 | 0.0021 | 0.0013 | 0.0011 | 0.0015 | 0.0009 | 0.0013 | 0.0007 | 0.0005 | 0.0005 |
Nucleus | 0.2203 | 0.2172 | 0.2601 | 0.3427 | 0.2654 | 0.3794 | 0.1423 | 0.2015 | 0.3025 | 0.2096 | 0.3264 | 0.2451 | 0.1075 | 0.366 | 0.2809 | 0.293 | 0.1657 | 0.2532 |
Peroxisomes | 0.0319 | 0.001 | 0.0043 | 0.0037 | 0.0005 | 0.0002 | 0.0279 | 0.0008 | 0.0004 | 0.0116 | 0.025 | 0.0029 | 0.002 | 0.0001 | 0.0194 | 0.0316 | 0.0007 | 0.0002 |
Punctate Nuclear | 0.0288 | 0.0165 | 0.0139 | 0.0141 | 0.0087 | 0.0227 | 0.0273 | 0.0191 | 0.0095 | 0.0232 | 0.0115 | 0.0087 | 0.0075 | 0.0067 | 0.0086 | 0.0034 | 0.0368 | 0.0113 |
Vacuole | 0.0059 | 0.0019 | 0.0055 | 0.0016 | 0.0029 | 0.0033 | 0.0059 | 0.0022 | 0.0015 | 0.0151 | 0.0035 | 0.0014 | 0.0079 | 0.0019 | 0.0016 | 0.0007 | 0.0022 | 0.0015 |
Vacuole Periphery | 0.0021 | 0.0002 | 0.0009 | 0.0004 | 0.0005 | 0.0006 | 0.0014 | 0.0003 | 0.0004 | 0.0013 | 0.0006 | 0.0003 | 0.0011 | 0.0003 | 0.0005 | 0.0002 | 0.001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.8812 | 15.7191 | 16.5244 | 15.8636 | 16.624 | 16.8421 | 17.3836 | 10.7271 | 10.2657 | 19.5931 |
Translational Efficiency | 1.1628 | 1.2474 | 0.9329 | 0.726 | 1.0431 | 0.8626 | 0.8024 | 0.8479 | 0.8846 | 0.7519 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
72 | 1038 | 41 | 1427 | 1797 | 650 | 2011 | 91 | 1869 | 1688 | 2052 | 1518 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 759.93 | 834.85 | 931.18 | 817.14 | 790.64 | 814.34 | 957.11 | 936.75 | 789.46 | 826.95 | 956.59 | 824.31 |
Standard Deviation | 91.27 | 131.08 | 121.88 | 129.07 | 107.77 | 126.89 | 121.47 | 132.04 | 107.34 | 129.87 | 121.53 | 132.33 |
Intensity Change Log 2 | — | 0.135650 | 0.293194 | 0.104717 | — | 0.042610 | 0.275664 | 0.244643 | — | 0.088959 | 0.284282 | 0.177760 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000076 | 0.000435 | 0.000663 | 0.001673 | 0.000094 | 0.000440 | 0.000314 | 0.000467 | 0.000093 | 0.000437 | 0.000321 | 0.001601 |
Bud Neck | 0.000647 | 0.003977 | 0.001727 | 0.011337 | 0.001916 | 0.012748 | 0.007253 | 0.010635 | 0.001867 | 0.007354 | 0.007143 | 0.011295 |
Bud Site | 0.000351 | 0.002673 | 0.001920 | 0.037225 | 0.001470 | 0.007743 | 0.006072 | 0.018311 | 0.001427 | 0.004625 | 0.005989 | 0.036092 |
Cell Periphery | 0.000195 | 0.000133 | 0.000257 | 0.000212 | 0.000115 | 0.000158 | 0.000170 | 0.000227 | 0.000118 | 0.000142 | 0.000171 | 0.000213 |
Cytoplasm | 0.323883* | 0.248543* | 0.108427* | 0.393412* | 0.302498* | 0.268137* | 0.241692* | 0.332430* | 0.303322* | 0.256088* | 0.239029* | 0.389756* |
Cytoplasmic Foci | 0.047220 | 0.028593 | 0.022855 | 0.024759 | 0.021938 | 0.069089 | 0.002649 | 0.014977 | 0.022912 | 0.044187 | 0.003053 | 0.024173 |
Eisosomes | 0.000066 | 0.000035 | 0.000106 | 0.000046 | 0.000022 | 0.000067 | 0.000021 | 0.000081 | 0.000024 | 0.000047 | 0.000023 | 0.000048 |
Endoplasmic Reticulum | 0.001749 | 0.004244 | 0.006966 | 0.002613 | 0.001706 | 0.003676 | 0.003680 | 0.008967 | 0.001707 | 0.004026 | 0.003746 | 0.002994 |
Endosome | 0.002850 | 0.003027 | 0.003570 | 0.023572 | 0.001109 | 0.009587 | 0.001177 | 0.002868 | 0.001176 | 0.005553 | 0.001225 | 0.022331 |
Golgi | 0.000171 | 0.001150 | 0.000133 | 0.012639 | 0.000427 | 0.002454 | 0.000724 | 0.000100 | 0.000417 | 0.001652 | 0.000713 | 0.011888 |
Lipid Particles | 0.004744 | 0.002116 | 0.004215 | 0.001434 | 0.001297 | 0.002945 | 0.000444 | 0.001772 | 0.001430 | 0.002435 | 0.000519 | 0.001454 |
Mitochondria | 0.000266 | 0.001605 | 0.000540 | 0.007755 | 0.000862 | 0.003826 | 0.001528 | 0.000591 | 0.000839 | 0.002460 | 0.001508 | 0.007326 |
Mitotic Spindle | 0.000036 | 0.003520 | 0.018393 | 0.082871 | 0.000952 | 0.006673 | 0.010195 | 0.017714 | 0.000917 | 0.004734 | 0.010359 | 0.078965 |
None | 0.006037 | 0.007469 | 0.004281 | 0.015150 | 0.008977 | 0.004478 | 0.010072 | 0.006267 | 0.008864 | 0.006317 | 0.009956 | 0.014617 |
Nuclear Periphery | 0.000459 | 0.001018 | 0.001827 | 0.001754 | 0.000947 | 0.001018 | 0.001894 | 0.001852 | 0.000928 | 0.001018 | 0.001892 | 0.001760 |
Nuclear Periphery Foci | 0.005938 | 0.001470 | 0.001852 | 0.001900 | 0.000664 | 0.001675 | 0.000553 | 0.001458 | 0.000867 | 0.001549 | 0.000579 | 0.001874 |
Nucleolus | 0.001400 | 0.001831 | 0.002158 | 0.001687 | 0.001083 | 0.003231 | 0.000626 | 0.000643 | 0.001095 | 0.002370 | 0.000657 | 0.001624 |
Nucleus | 0.588501* | 0.667439* | 0.759261* | 0.279750* | 0.645405* | 0.533880* | 0.689204* | 0.431777* | 0.643213* | 0.616009* | 0.690604* | 0.288863* |
Peroxisomes | 0.000493 | 0.003187 | 0.001125 | 0.002506 | 0.000385 | 0.009480 | 0.000118 | 0.000256 | 0.000389 | 0.005610 | 0.000138 | 0.002371 |
Vacuole | 0.014689 | 0.016998 | 0.059454 | 0.092969 | 0.007929 | 0.056736 | 0.021184 | 0.148299 | 0.008190 | 0.032300 | 0.021949 | 0.096286 |
Vacuole Periphery | 0.000226 | 0.000537 | 0.000271 | 0.004736 | 0.000206 | 0.001961 | 0.000430 | 0.000308 | 0.000207 | 0.001085 | 0.000427 | 0.004470 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.40 | -3.33 | -4.71 | -2.30 | 1.00 | -7.92 | -14.77 | -4.95 | -2.58 | -2.83 | -9.50 | -15.10 | -4.94 | -2.50 | -2.61 |
Bud Neck | -5.92 | -2.97 | -10.66 | -5.92 | -5.28 | -6.18 | -9.93 | -5.24 | 1.31 | -0.97 | -7.43 | -10.07 | -10.36 | -2.37 | -2.10 |
Bud Site | -5.81 | -3.51 | -8.98 | -7.57 | -6.94 | -6.07 | -3.89 | -2.23 | -0.66 | -1.48 | -5.48 | -4.01 | -8.46 | -6.65 | -6.60 |
Cell Periphery | 1.57 | -1.38 | 1.35 | -0.75 | 2.56 | -2.67 | -1.78 | -3.09 | -0.75 | -2.05 | -2.66 | -1.80 | -2.83 | -0.11 | -0.75 |
Cytoplasm | 2.23 | 6.13 | 1.05 | -3.91 | -10.62 | 2.99 | 14.84 | 4.61 | 3.16 | 0.11 | 5.45 | 15.44 | 2.17 | -2.96 | -12.02 |
Cytoplasmic Foci | 1.49 | 1.10 | 2.82 | 5.93 | 0.85 | -9.22 | 13.19 | 5.91 | 11.72 | -4.11 | -7.19 | 13.02 | 6.17 | 11.98 | -8.88 |
Eisosomes | 1.86 | -2.74 | 0.33 | -7.56 | 3.73 | -9.72 | -5.11 | -9.07 | -4.24 | -8.24 | -9.30 | -4.97 | -17.27 | -4.78 | -13.98 |
Endoplasmic Reticulum | -3.77 | -4.05 | -4.00 | -0.13 | 2.97 | -5.68 | -14.29 | -6.06 | -4.44 | -3.57 | -10.20 | -14.75 | -13.75 | -2.09 | 1.63 |
Endosome | -0.06 | -0.81 | -3.57 | -4.40 | -3.28 | -5.90 | 0.42 | -4.99 | 3.87 | -5.17 | -6.60 | 0.50 | -6.78 | -2.20 | -6.87 |
Golgi | -1.73 | 0.11 | -4.31 | -2.71 | -4.33 | -2.24 | 0.28 | 1.68 | 2.65 | 1.12 | -2.36 | 0.27 | -3.92 | -2.23 | -3.98 |
Lipid Particles | 1.52 | 0.31 | 1.96 | 2.88 | 3.46 | -4.70 | 5.12 | -2.91 | 0.69 | -4.83 | -4.08 | 5.12 | -0.01 | 4.55 | -7.26 |
Mitochondria | -4.05 | -3.78 | -4.29 | -2.17 | -3.73 | -4.05 | -2.63 | 0.21 | 4.24 | 3.74 | -4.11 | -2.75 | -3.34 | -0.99 | -2.43 |
Mitotic Spindle | -2.46 | -1.01 | -10.86 | -9.43 | -1.90 | -2.81 | -6.85 | -2.03 | -1.29 | -0.80 | -3.15 | -6.94 | -10.76 | -9.36 | -7.60 |
None | -1.05 | 1.30 | -2.46 | -1.99 | -3.14 | 4.58 | 1.22 | 3.73 | 0.41 | 3.22 | 2.86 | 1.23 | -0.39 | -2.94 | -1.53 |
Nuclear Periphery | -4.38 | -5.33 | -15.79 | -14.54 | -2.25 | -0.54 | -19.62 | -6.72 | -6.11 | -2.12 | -1.33 | -20.18 | -17.36 | -15.20 | -3.25 |
Nuclear Periphery Foci | 1.36 | 1.20 | 1.39 | 0.35 | 1.17 | -2.35 | 0.64 | -2.62 | -1.09 | -2.75 | -2.59 | 1.47 | -2.50 | 0.31 | -4.78 |
Nucleolus | -0.85 | -0.61 | 0.41 | 1.52 | 0.88 | -2.73 | 2.41 | 2.61 | 3.42 | 0.88 | -3.22 | 2.40 | -0.25 | 2.85 | -2.07 |
Nucleus | -2.00 | -2.87 | 4.44 | 20.74 | 8.51 | 7.59 | -9.34 | 6.22 | 2.79 | 8.70 | 2.91 | -9.75 | 22.10 | 18.18 | 33.17 |
Peroxisomes | -2.94 | -1.04 | -1.72 | 2.51 | 0.59 | -4.63 | 4.57 | 1.80 | 4.70 | -3.04 | -5.53 | 4.19 | -2.76 | 4.96 | -4.58 |
Vacuole | -1.21 | -3.34 | -21.39 | -24.51 | -4.30 | -11.54 | -13.86 | -9.25 | -7.50 | -8.30 | -12.64 | -14.16 | -28.23 | -22.65 | -22.15 |
Vacuole Periphery | -2.11 | -0.81 | -4.49 | -3.33 | -4.35 | -3.32 | -0.41 | -0.27 | 3.27 | 0.22 | -4.04 | -0.42 | -4.73 | -1.30 | -4.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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