Standard name
Human Ortholog
Description Essential component of the TIM23 complex; involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel; TIM23 complex is short for the translocase of the inner mitochondrial membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 – – – 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0.07 0 0.06 0 0 0 0 – 0 0 0 0.33 0.32 0.44 – – – 0.06 0.07 0.07 0.09 0.11 0.11
Mitochondria 0.86 0.89 0.93 0.93 0.93 0.95 0.91 0.95 – 0.93 0.94 0.94 0.8 0.77 0.71 – – – 0.73 0.79 0.78 0.7 0.69 0.65
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.06 0 0.07 0.07 0.07 0.08 – 0.11 0.06 0.1 0 0 0.05 – – – 0 0 0 0 0 0
Nucleolus 0.2 0 0.1 0 0.05 0.12 0.13 0.12 – 0.14 0.11 0.1 0 0.05 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0.05 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.16 0 0.05 0.09 0 0.05 0 – 0 0 0 0.16 0.15 0.17 – – – 0 0.05 0 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 1 0 1 2 1 – 1 0 0 4 14 5 – – – 6 11 4 5 14 16
Bud 2 1 6 1 2 8 12 10 – 4 10 4 1 5 0 – – – 0 2 0 1 5 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 2 0 0 1 0 2
Bud Site 0 0 1 0 0 2 2 2 – 4 0 2 0 0 0 – – – – – – – – –
Cell Periphery 2 15 4 5 0 1 4 2 – 3 1 3 8 3 3 – – – 0 0 1 0 0 0
Cytoplasm 1 1 5 3 4 10 7 7 – 4 5 2 5 3 3 – – – 0 1 0 0 0 0
Endoplasmic Reticulum 3 1 0 1 1 2 1 0 – 1 1 2 1 0 2 – – – 0 0 1 0 2 1
Endosome 1 3 3 0 2 0 0 0 – 0 0 0 1 1 2 – – – 5 5 4 2 6 4
Golgi 3 26 2 8 5 0 0 0 – 1 0 1 93 91 132 – – – 11 29 15 14 35 32
Mitochondria 141 339 202 132 200 283 373 395 – 272 220 251 227 217 212 – – – 133 325 178 106 219 186
Nucleus 1 1 0 1 0 2 3 2 – 0 0 0 0 0 0 – – – 0 0 1 0 0 0
Nuclear Periphery 9 10 12 3 15 20 28 32 – 31 15 27 3 4 15 – – – 2 3 0 1 2 3
Nucleolus 32 16 21 5 11 35 52 48 – 42 25 26 12 14 10 – – – 4 2 1 1 0 3
Peroxisomes 1 1 7 0 1 0 3 2 – 2 1 3 7 4 2 – – – 6 9 12 5 11 12
SpindlePole 0 2 1 1 0 0 2 2 – 0 2 2 1 1 0 – – – 0 1 0 0 0 0
Vac/Vac Membrane 18 63 0 7 19 8 19 15 – 12 3 8 46 43 51 – – – 6 19 2 10 13 15
Unique Cell Count 164 383 218 142 216 298 410 415 291 235 267 285 281 299 182 414 229 153 318 286
Labelled Cell Count 215 480 266 168 260 372 508 518 377 283 331 409 400 437 182 414 229 153 318 286


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.4 16.6 13.3 14.0 11.7 10.2 9.0 9.1 – 8.1 8.3 8.2 17.0 16.9 16.2 – – – 17.9 18.5 18.7
Std Deviation (1e-4) 4.7 3.6 3.1 2.9 2.7 2.3 2.2 2.5 – 2.2 2.2 2.2 3.4 3.2 2.7 – – – 4.1 4.3 4.3
Intensity Change (Log2) – – – 0.07 -0.19 -0.38 -0.57 -0.55 – -0.71 -0.68 -0.7 0.36 0.34 0.28 – – – 0.43 0.47 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 2.6 0 – – –
Bud 0 0 0 0.1 -0.3 – 0 0.9 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0.7 0 0 – – –
Cytoplasm 0 0 0.7 0 0 – 0 0 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 9.0 9.0 11.1 – – –
Mitochondria 0.1 0 1.1 -0.7 1.3 – 0.4 0.4 0.6 -4.1 -4.7 -6.1 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery -1.6 0.6 0.6 0.6 1.0 – 2.1 0.4 1.9 -2.9 -2.6 -0.2 – – –
Nucleolus -2.2 -1.8 0.8 1.1 0.7 – 1.6 0.4 0 -2.4 -2.0 -3.0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 -0.5 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole 0 4.5 0 3.2 2.8 – 3.0 0 0 6.2 6.0 6.4 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.234 21.3364 20.0315 19.7673 15.2139 19.8144 26.2264 23.5938 24.056 23.5181 20.3766 23.6494 25.1713 23.4523 23.1398 22.0386 22.4975 22.7649
Actin 0.0189 0.0033 0.001 0.0009 0.0045 0.0004 0.0151 0.0017 0.0009 0.0004 0 0.0103 0.0048 0.003 0.0007 0.0001 0.0001 0.0013
Bud 0.0002 0.0009 0.0003 0.0001 0.0002 0 0.0002 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Bud Neck 0.0004 0.0002 0 0 0.0001 0 0.001 0.0001 0 0 0 0 0.0003 0.0001 0 0 0 0.0002
Bud Periphery 0.0003 0.0011 0.0005 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0
Bud Site 0.001 0.0016 0.0002 0.0003 0.0004 0 0.0007 0.0003 0.0002 0 0 0.0001 0.0003 0.0003 0.0001 0 0 0
Cell Periphery 0.0005 0.0022 0.001 0.0001 0.0002 0.0002 0.0003 0.0009 0.0001 0.0001 0.0003 0.0001 0.0005 0.0004 0.0005 0.0005 0 0.0001
Cytoplasm 0.0065 0.0003 0.0001 0.0003 0.0025 0 0.003 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0.0005
Cytoplasmic Foci 0.0172 0.0012 0.002 0.005 0.0318 0.0004 0.0038 0.0009 0.0001 0.002 0.0001 0.0003 0.0009 0.0015 0.0002 0.0003 0 0.0002
Eisosomes 0.0006 0.0004 0.0002 0.0001 0.0005 0.0002 0.0003 0.0005 0.0002 0.0004 0.0001 0.0002 0.0003 0.0002 0.0003 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0024 0.0002 0 0.0003 0.0009 0 0.0029 0 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0008
Endosome 0.0149 0.0057 0.0049 0.0025 0.0319 0.0039 0.0081 0.0007 0.0004 0.0053 0.0002 0.0016 0.0012 0.0051 0.0027 0.0012 0.0001 0.003
Golgi 0.006 0.0076 0.0068 0.0021 0.0047 0.0079 0.006 0.0031 0.0005 0.0105 0.0002 0.0212 0.0061 0.0224 0.0027 0.0026 0.0003 0.0057
Lipid Particles 0.0286 0.0092 0.0131 0.0159 0.0154 0.0098 0.0097 0.0094 0.0011 0.03 0.0105 0.0086 0.0107 0.0119 0.003 0.0036 0.0001 0.002
Mitochondria 0.8315 0.8995 0.9267 0.9544 0.8349 0.9556 0.9175 0.9389 0.9905 0.9436 0.9557 0.9528 0.9484 0.9271 0.9679 0.9607 0.9987 0.9703
None 0.0092 0 0 0.0006 0.0446 0 0.0024 0 0 0 0 0 0.0002 0 0 0.0001 0 0.0003
Nuclear Periphery 0.0086 0.0034 0.0001 0.0025 0.0015 0.0001 0.0073 0.0001 0 0 0.0001 0 0.0002 0.0008 0.0001 0.0001 0 0.0053
Nucleolus 0.0062 0.0015 0.0003 0.0013 0.0017 0.0002 0.0009 0.0023 0.0002 0.0001 0.0027 0.0001 0.0016 0.0006 0.0016 0.0044 0 0.0001
Nucleus 0.0041 0.0004 0.0001 0.0009 0.001 0.0001 0.0021 0.0023 0 0 0.0004 0 0.0003 0.0011 0.0001 0.001 0 0.0017
Peroxisomes 0.0081 0.003 0.0017 0.0013 0.009 0.0004 0.0036 0.0088 0.0026 0.0012 0.0006 0.0011 0.0013 0.002 0.0007 0.0014 0.0001 0.0002
Punctate Nuclear 0.0153 0 0 0.0073 0.0051 0 0.0052 0.0053 0 0 0 0 0.0004 0 0 0 0 0.0014
Vacuole 0.0044 0.0212 0.0127 0.0007 0.0037 0.0053 0.0024 0.0044 0.0007 0.0013 0.003 0.0003 0.0043 0.0051 0.0045 0.0046 0 0.0006
Vacuole Periphery 0.0153 0.037 0.028 0.0034 0.0052 0.0154 0.007 0.0199 0.0022 0.0051 0.026 0.0029 0.0179 0.0177 0.0145 0.0191 0.0002 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 111.33 109.1059 130.1945 102.5392 98.3679 138.4674 120.3795 102.6291 145.221 115.4835
Translational Efficiency 1.2807 1.0576 0.9915 1.6377 1.4086 1.2373 1.1351 1.4694 1.1806 1.2957

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
297 786 192 1162 1157 1635 1936 1446 1454 2421 2128 2608

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 971.59 1346.23 1500.66 1460.65 1236.41 1384.64 1601.71 1454.60 1182.32 1372.17 1592.59 1457.30
Standard Deviation 177.00 291.85 261.00 274.40 312.48 289.07 285.19 284.24 309.03 290.53 284.57 279.91
Intensity Change Log 2 — 0.470505 0.627178 0.588191 — 0.163354 0.373456 0.234465 — 0.306620 0.490631 0.400879

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000990 0.016323 0.031760 0.027372 0.003827 0.008467 0.011698 0.018007 0.003248 0.011018 0.013509 0.022179
Bud Neck 0.001472 0.003471 0.007145 0.003510 0.002923 0.002725 0.000992 0.001809 0.002626 0.002967 0.001547 0.002567
Bud Site 0.000961 0.002932 0.003443 0.004635 0.002429 0.002329 0.001456 0.002546 0.002129 0.002525 0.001635 0.003477
Cell Periphery 0.000169 0.000192 0.000284 0.000314 0.000511 0.000154 0.000122 0.000375 0.000441 0.000166 0.000136 0.000348
Cytoplasm 0.000543 0.000547 0.000433 0.000778 0.000405 0.000474 0.000207 0.000239 0.000433 0.000498 0.000227 0.000479
Cytoplasmic Foci 0.027004 0.040066 0.058123 0.018159 0.030361 0.032892 0.010878 0.009730 0.029675 0.035221 0.015141 0.013486
Eisosomes 0.000091 0.000706 0.000782 0.000888 0.000436 0.000382 0.000618 0.001106 0.000365 0.000487 0.000633 0.001009
Endoplasmic Reticulum 0.000312 0.000539 0.001456 0.000674 0.000562 0.000407 0.000261 0.000566 0.000511 0.000450 0.000369 0.000614
Endosome 0.012874 0.014037 0.028120 0.008598 0.012146 0.011952 0.002548 0.004255 0.012295 0.012629 0.004855 0.006190
Golgi 0.023912 0.077208 0.155281* 0.066100 0.033010 0.071691 0.042743 0.045093 0.031152 0.073482 0.052897 0.054453
Lipid Particles 0.007537 0.016136 0.024149 0.004610 0.013143 0.017517 0.004394 0.004777 0.011998 0.017069 0.006177 0.004702
Mitochondria 0.885234* 0.778242* 0.621424* 0.826915* 0.848140* 0.810437* 0.902503* 0.890922* 0.855717* 0.799985* 0.877142* 0.862403*
Mitotic Spindle 0.003923 0.001661 0.006719 0.004374 0.002392 0.001936 0.000888 0.001294 0.002705 0.001847 0.001414 0.002666
None 0.001179 0.001371 0.002320 0.001330 0.002552 0.001409 0.001893 0.001482 0.002271 0.001397 0.001931 0.001414
Nuclear Periphery 0.000942 0.000469 0.003457 0.000519 0.002080 0.001149 0.000456 0.000241 0.001848 0.000928 0.000726 0.000365
Nuclear Periphery Foci 0.001094 0.000452 0.001436 0.000304 0.001666 0.001074 0.000208 0.000162 0.001549 0.000872 0.000319 0.000225
Nucleolus 0.001253 0.001299 0.000489 0.000298 0.003716 0.000995 0.000229 0.000114 0.003213 0.001094 0.000253 0.000196
Nucleus 0.000362 0.000178 0.000095 0.000121 0.000659 0.000364 0.000034 0.000036 0.000598 0.000304 0.000039 0.000074
Peroxisomes 0.012659 0.026266 0.014870 0.014643 0.017814 0.010815 0.004754 0.007985 0.016761 0.015831 0.005667 0.010951
Vacuole 0.007866 0.010556 0.014485 0.006097 0.011102 0.011260 0.003510 0.003150 0.010441 0.011032 0.004500 0.004463
Vacuole Periphery 0.009625 0.007351 0.023728 0.009760 0.010126 0.011569 0.009608 0.006112 0.010024 0.010199 0.010882 0.007737

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.00 -4.33 -9.39 -3.11 0.57 -3.65 -5.62 -7.50 -4.59 -2.93 -6.74 -7.33 -11.28 -5.90 -4.23
Bud Neck -2.98 -4.11 -3.76 -0.08 2.63 0.28 3.74 2.00 1.87 -3.13 -0.65 2.45 0.12 0.99 -3.80
Bud Site -3.16 -2.91 -4.56 -1.81 -1.08 0.08 1.44 -0.14 -0.21 -1.40 -0.77 0.85 -2.12 -1.51 -2.89
Cell Periphery -0.34 -1.43 -1.98 -3.04 -0.54 2.45 2.68 0.90 -4.66 -5.28 2.36 2.61 0.77 -5.76 -6.50
Cytoplasm -0.01 0.50 -0.62 -0.71 -1.02 -0.76 3.57 3.02 2.68 -0.90 -0.81 3.25 -0.33 0.07 -1.62
Cytoplasmic Foci -2.48 -3.73 1.97 6.62 5.49 -0.88 8.06 8.53 11.96 0.97 -2.19 6.43 7.56 12.71 1.28
Eisosomes -9.97 -5.56 -7.05 -1.42 -0.63 1.05 -3.12 -5.83 -6.60 -4.30 -2.78 -5.24 -7.57 -6.36 -4.37
Endoplasmic Reticulum -2.37 -2.36 -2.88 -1.00 1.58 1.76 3.48 -0.03 -2.12 -4.17 0.82 1.70 -1.15 -2.32 -3.01
Endosome -0.39 -2.90 1.70 2.69 4.08 0.11 6.24 4.95 7.84 -3.20 -0.22 5.34 4.40 6.73 -1.92
Golgi -7.58 -8.18 -7.02 1.58 5.55 -8.37 -2.53 -2.85 5.48 -0.57 -10.85 -5.83 -6.59 4.72 -0.40
Lipid Particles -3.34 -3.11 1.58 5.52 3.81 -2.03 5.62 4.95 7.01 -0.37 -2.92 4.16 5.44 8.95 1.62
Mitochondria 6.10 8.96 3.72 -3.61 -7.55 3.56 -5.98 -4.42 -8.86 1.59 6.11 -2.51 -0.80 -8.18 2.13
Mitotic Spindle 1.90 -1.47 -0.31 -3.15 1.37 0.56 2.36 1.40 0.87 -0.78 1.30 2.21 0.06 -1.38 -2.48
None -0.99 -4.08 -0.81 0.37 4.34 1.82 1.05 1.70 -0.61 3.21 1.75 0.68 1.72 -0.20 4.98
Nuclear Periphery 0.83 -2.01 0.73 -0.40 2.61 1.13 2.08 2.37 3.17 2.16 1.40 1.75 2.36 2.81 2.54
Nuclear Periphery Foci 1.33 -0.56 1.63 1.71 3.13 1.45 4.26 4.41 4.05 0.97 2.08 4.21 4.57 4.16 1.62
Nucleolus -0.05 1.18 1.50 1.71 1.08 2.31 3.08 3.19 2.71 1.62 2.22 3.25 3.32 3.09 0.77
Nucleus 1.09 1.66 1.42 0.74 -0.57 1.23 3.84 3.83 1.97 -0.31 1.64 4.19 3.87 1.98 -1.60
Peroxisomes -3.34 -0.43 -0.62 3.20 0.04 2.78 5.92 3.94 1.47 -2.14 0.42 5.84 2.79 2.74 -3.97
Vacuole -0.79 -1.56 0.57 2.42 2.68 -0.11 5.97 5.98 6.76 0.51 -0.42 4.84 4.86 6.50 0.05
Vacuole Periphery 1.04 -3.50 -0.06 -1.98 3.88 -1.18 0.43 3.80 5.36 3.54 -0.17 -0.76 2.37 3.04 3.31
Standard name
Human Ortholog
Description Essential component of the TIM23 complex; involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel; TIM23 complex is short for the translocase of the inner mitochondrial membrane
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Tim23

Tim23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available