ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 11 | 22 | 19 | 34 | 23 | 23 | 34 | 37 | 18 | 34 | 3 | 6 | 4 | 9 | 6 | 4 | 1 | 0 | 3 | 0 | 2 | 3 |
Bud | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 4 | 4 | 4 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 8 | 11 | 8 | 9 | 13 | 8 | 13 | 13 | 5 | 22 | 14 | 44 | 48 | 53 | 15 | 4 | 1 | 0 | 2 | 0 | 1 | 1 |
Cytoplasm | 3 | 4 | 8 | 11 | 11 | 7 | 11 | 17 | 8 | 9 | 10 | 13 | 6 | 13 | 16 | 25 | 9 | 3 | 1 | 1 | 1 | 4 | 8 | 8 |
Endoplasmic Reticulum | 80 | 31 | 171 | 120 | 137 | 157 | 264 | 222 | 190 | 207 | 140 | 174 | 150 | 236 | 217 | 2 | 1 | 0 | 98 | 64 | 175 | 92 | 166 | 180 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2 | 0 | 35 | 57 | 40 | 19 | 11 | 18 | 9 | 5 | 2 | 6 | 12 | 8 | 8 | 45 | 11 | 7 | 0 | 0 | 1 | 0 | 2 | 0 |
Mitochondria | 2 | 0 | 7 | 33 | 16 | 41 | 48 | 32 | 47 | 67 | 67 | 83 | 1 | 0 | 0 | 6 | 3 | 1 | 0 | 0 | 4 | 1 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 13 | 3 | 0 | 16 | 28 | 15 | 18 | 27 | 17 | 23 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 1 | 2 | 9 | 5 | 19 | 22 | 6 | 10 | 29 | 0 | 3 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 2 | 3 | 7 |
Unique Cell Count | 86 | 34 | 189 | 160 | 169 | 190 | 312 | 292 | 258 | 267 | 202 | 281 | 157 | 263 | 250 | 125 | 43 | 18 | 105 | 73 | 192 | 105 | 188 | 208 |
Labelled Cell Count | 91 | 41 | 255 | 258 | 234 | 292 | 403 | 356 | 343 | 375 | 274 | 389 | 188 | 313 | 297 | 145 | 48 | 20 | 105 | 73 | 192 | 105 | 188 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 24.0 | 28.9 | 26.1 | 25.3 | 21.8 | 18.4 | 16.9 | 16.4 | 15.9 | 15.2 | 13.9 | 14.3 | 29.4 | 28.2 | 26.6 | 36.0 | 34.2 | 36.2 | 28.7 | 28.6 | 29.5 |
Std Deviation (1e-4) | 4.2 | 5.7 | 4.8 | 3.8 | 4.2 | 2.7 | 2.9 | 2.9 | 3.0 | 2.9 | 2.7 | 2.7 | 5.7 | 5.0 | 4.7 | 8.3 | 7.4 | 8.0 | 5.0 | 5.8 | 6.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.26 | -0.51 | -0.63 | -0.67 | -0.72 | -0.78 | -0.91 | -0.86 | 0.17 | 0.11 | 0.03 | 0.46 | 0.39 | 0.47 | 0.14 | 0.13 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.5 | 1.8 | 3.6 | 0.7 | 0.9 | 2.6 | 2.8 | 1.2 | 2.3 | -1.8 | -2.0 | -2.4 | 0.5 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.1 | 0.2 | 0.2 | 0 | -0.9 | 0.4 | 0.3 | -1.0 | 1.6 | 1.8 | 4.1 | 4.7 | 8.4 | 0 | 0 |
Cytoplasm | 1.1 | 1.0 | -0.3 | -0.4 | 0.8 | -0.6 | -0.5 | 0.3 | 0.2 | -0.2 | 0.4 | 1.0 | 4.5 | 0 | 0 |
Endoplasmic Reticulum | -3.9 | -2.6 | -2.2 | -1.9 | -4.0 | -4.5 | -3.6 | -5.2 | -6.9 | 1.8 | -0.3 | -1.2 | -15.5 | -11.9 | -9.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.6 | 1.2 | -2.4 | -5.6 | -4.2 | -5.3 | -6.2 | -5.9 | -6.2 | -2.9 | -5.5 | -5.3 | 3.5 | 1.0 | 0 |
Mitochondria | 4.9 | 2.2 | 5.2 | 4.1 | 2.8 | 4.7 | 6.1 | 7.4 | 7.0 | 0 | 0 | 0 | 0.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.2 | -3.5 | 0.6 | 0.8 | -0.8 | 0 | 1.2 | 0.6 | 0.5 | -2.5 | -3.6 | -3.5 | -2.6 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | 3.8 | 0 | 2.6 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.1391 | 25.2596 | 26.2554 | 26.1359 | 21.6515 | 24.9662 | 26.9134 | 30.5622 | 26.6772 | 25.9806 | 22.6843 | 28.3889 | 32.9326 | 30.3409 | 30.4601 | 28.8349 | 26.482 | 29.9674 |
Actin | 0.0235 | 0.0005 | 0.0346 | 0.0002 | 0.0562 | 0.0036 | 0.0176 | 0.0023 | 0.0153 | 0.0168 | 0.0447 | 0.0018 | 0.0009 | 0.0117 | 0.0003 | 0.0005 | 0.0001 | 0.0002 |
Bud | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0014 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0081 | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0.0013 | 0.0004 | 0.0007 | 0.0004 | 0.0019 | 0.0133 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0.0002 |
Bud Periphery | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0003 | 0.0006 | 0.001 | 0.0006 | 0.0004 | 0.0026 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Bud Site | 0.0016 | 0 | 0.001 | 0 | 0.0025 | 0.0001 | 0.0009 | 0.0004 | 0.0024 | 0.0007 | 0.0093 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0024 | 0.006 | 0.0027 | 0.0084 | 0.0039 | 0.0015 | 0.011 | 0.0143 | 0.0071 | 0.0014 | 0.0021 | 0.0021 | 0.0059 | 0.004 | 0.0097 | 0.0017 | 0.0016 | 0.0008 |
Cytoplasm | 0.0054 | 0.003 | 0.0007 | 0.001 | 0.0025 | 0.0024 | 0.0246 | 0.0247 | 0.0136 | 0.0007 | 0.0722 | 0.0086 | 0.0053 | 0.0032 | 0.0056 | 0.001 | 0.0003 | 0.0006 |
Cytoplasmic Foci | 0.0016 | 0.0002 | 0.0006 | 0.0001 | 0.0028 | 0.0009 | 0.0147 | 0.0008 | 0.0071 | 0.0029 | 0.0164 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0012 | 0 | 0 |
Eisosomes | 0.0003 | 0.0014 | 0.0006 | 0.0003 | 0.0045 | 0.0003 | 0.0008 | 0.0002 | 0.0004 | 0.0003 | 0.0012 | 0.0004 | 0.0002 | 0.0008 | 0.0014 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.7843 | 0.9549 | 0.9225 | 0.9402 | 0.7623 | 0.8988 | 0.6461 | 0.9053 | 0.8664 | 0.8788 | 0.6493 | 0.8927 | 0.8557 | 0.9259 | 0.9524 | 0.9538 | 0.9852 | 0.9251 |
Endosome | 0.0084 | 0.0015 | 0.001 | 0.001 | 0.0092 | 0.0035 | 0.016 | 0.005 | 0.0121 | 0.0077 | 0.0199 | 0.0022 | 0.0029 | 0.0007 | 0.0006 | 0.0012 | 0.0006 | 0.0004 |
Golgi | 0.0032 | 0.0024 | 0.0024 | 0.004 | 0.0146 | 0.003 | 0.0055 | 0.0046 | 0.0092 | 0.0086 | 0.0165 | 0.0024 | 0.0015 | 0.0012 | 0.001 | 0.0005 | 0.0003 | 0.0007 |
Lipid Particles | 0.0023 | 0.005 | 0.0008 | 0.0025 | 0.0274 | 0.0043 | 0.0171 | 0.0171 | 0.0122 | 0.0079 | 0.0232 | 0.0049 | 0.0027 | 0.0047 | 0.0044 | 0.0028 | 0.0002 | 0.0015 |
Mitochondria | 0.003 | 0.0005 | 0.0006 | 0.0001 | 0.0016 | 0.0021 | 0.0174 | 0.0021 | 0.0077 | 0.0365 | 0.0466 | 0.0027 | 0.0012 | 0.0044 | 0.0003 | 0.0038 | 0 | 0 |
None | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0002 | 0.0088 | 0.0001 | 0.0012 | 0.0013 | 0.0013 | 0.0014 | 0.0004 | 0.0001 | 0.0002 | 0.0014 | 0 | 0 |
Nuclear Periphery | 0.1342 | 0.0186 | 0.0283 | 0.0391 | 0.058 | 0.0668 | 0.1706 | 0.0128 | 0.0292 | 0.0187 | 0.0503 | 0.0711 | 0.11 | 0.037 | 0.0187 | 0.02 | 0.0102 | 0.0662 |
Nucleolus | 0.0003 | 0 | 0 | 0 | 0.0033 | 0.0001 | 0.0013 | 0.0001 | 0.0005 | 0.0007 | 0.0015 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0022 | 0 | 0 |
Nucleus | 0.0058 | 0.0005 | 0.0004 | 0.0002 | 0.0063 | 0.0047 | 0.0115 | 0.0008 | 0.0019 | 0.0008 | 0.0047 | 0.0027 | 0.0039 | 0.0019 | 0.0005 | 0.0018 | 0.0001 | 0.0011 |
Peroxisomes | 0.0006 | 0 | 0.0001 | 0 | 0.0011 | 0.0001 | 0.0061 | 0 | 0.0031 | 0.0028 | 0.0109 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.0013 | 0 | 0 | 0.0001 | 0.0343 | 0.0002 | 0.0048 | 0.0001 | 0.0006 | 0.0068 | 0.0051 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0 |
Vacuole | 0.0055 | 0.004 | 0.0013 | 0.0016 | 0.0021 | 0.0033 | 0.0142 | 0.0061 | 0.0048 | 0.002 | 0.0049 | 0.0021 | 0.0049 | 0.0017 | 0.003 | 0.0017 | 0.0009 | 0.0015 |
Vacuole Periphery | 0.006 | 0.001 | 0.0013 | 0.0007 | 0.0032 | 0.0024 | 0.0097 | 0.0012 | 0.0039 | 0.002 | 0.0025 | 0.0028 | 0.0036 | 0.002 | 0.0014 | 0.0049 | 0.0004 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.1309 | 105.5382 | 106.6274 | 131.1142 | 127.1655 | 126.1632 | 106.0939 | 117.4509 | 94.2761 | 140.8382 |
Translational Efficiency | 1.0287 | 1.1943 | 1.252 | 0.939 | 1.0321 | 1.0814 | 1.0148 | 0.9607 | 1.2118 | 0.9521 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
541 | 252 | 498 | 1339 | 111 | 1771 | 421 | 696 | 652 | 2023 | 919 | 2035 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1944.85 | 2318.68 | 2752.23 | 2777.30 | 2273.18 | 2153.64 | 2689.05 | 2651.51 | 2000.75 | 2174.20 | 2723.29 | 2734.28 |
Standard Deviation | 374.62 | 427.90 | 424.82 | 519.09 | 431.75 | 408.34 | 386.74 | 500.82 | 404.24 | 414.43 | 409.03 | 516.38 |
Intensity Change Log 2 | — | 0.253645 | 0.500942 | 0.514024 | — | -0.077935 | 0.242385 | 0.222102 | — | 0.084454 | 0.367377 | 0.364067 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000373 | 0.000593 | 0.000175 | 0.001563 | 0.001617 | 0.000360 | 0.002199 | 0.000171 | 0.000585 | 0.000389 | 0.001102 | 0.001087 |
Bud Neck | 0.000303 | 0.000258 | 0.001912 | 0.002148 | 0.002931 | 0.000351 | 0.008960 | 0.001450 | 0.000751 | 0.000340 | 0.005141 | 0.001909 |
Bud Site | 0.000548 | 0.004431 | 0.000786 | 0.005467 | 0.001960 | 0.001215 | 0.007186 | 0.002435 | 0.000789 | 0.001616 | 0.003718 | 0.004430 |
Cell Periphery | 0.002173 | 0.002577 | 0.007961 | 0.021750 | 0.011618 | 0.001798 | 0.010094 | 0.032553 | 0.003781 | 0.001895 | 0.008938 | 0.025445 |
Cytoplasm | 0.000131 | 0.002136 | 0.000614 | 0.001884 | 0.000035 | 0.001401 | 0.000848 | 0.000693 | 0.000114 | 0.001493 | 0.000721 | 0.001477 |
Cytoplasmic Foci | 0.000533 | 0.001177 | 0.000092 | 0.001476 | 0.000950 | 0.001269 | 0.000311 | 0.000489 | 0.000604 | 0.001258 | 0.000192 | 0.001138 |
Eisosomes | 0.000126 | 0.000222 | 0.000423 | 0.000594 | 0.001716 | 0.000273 | 0.000436 | 0.000429 | 0.000397 | 0.000266 | 0.000429 | 0.000538 |
Endoplasmic Reticulum | 0.964696* | 0.967245* | 0.941949* | 0.886083* | 0.932069* | 0.971777* | 0.889031* | 0.878557* | 0.959142* | 0.971212* | 0.917707* | 0.883509* |
Endosome | 0.005209 | 0.003702 | 0.005304 | 0.006690 | 0.008948 | 0.005932 | 0.015336 | 0.006086 | 0.005846 | 0.005654 | 0.009900 | 0.006483 |
Golgi | 0.011147 | 0.008865 | 0.004942 | 0.015905 | 0.014545 | 0.003088 | 0.004742 | 0.020541 | 0.011726 | 0.003808 | 0.004850 | 0.017491 |
Lipid Particles | 0.004625 | 0.002049 | 0.006656 | 0.022995 | 0.002503 | 0.005140 | 0.026705 | 0.024606 | 0.004264 | 0.004755 | 0.015840 | 0.023546 |
Mitochondria | 0.000473 | 0.000258 | 0.000216 | 0.001752 | 0.001639 | 0.000315 | 0.000216 | 0.000218 | 0.000671 | 0.000308 | 0.000216 | 0.001228 |
Mitotic Spindle | 0.000682 | 0.000169 | 0.000249 | 0.001366 | 0.000215 | 0.000482 | 0.001533 | 0.000164 | 0.000603 | 0.000443 | 0.000837 | 0.000955 |
None | 0.000110 | 0.000734 | 0.000038 | 0.000392 | 0.000052 | 0.001152 | 0.000040 | 0.000204 | 0.000101 | 0.001100 | 0.000039 | 0.000327 |
Nuclear Periphery | 0.001244 | 0.001026 | 0.008204 | 0.003174 | 0.003947 | 0.000819 | 0.004590 | 0.004464 | 0.001704 | 0.000844 | 0.006548 | 0.003615 |
Nuclear Periphery Foci | 0.002651 | 0.001071 | 0.007697 | 0.003882 | 0.004918 | 0.002415 | 0.014005 | 0.004618 | 0.003037 | 0.002247 | 0.010586 | 0.004133 |
Nucleolus | 0.000007 | 0.000005 | 0.000009 | 0.000107 | 0.000024 | 0.000003 | 0.000006 | 0.000013 | 0.000010 | 0.000003 | 0.000008 | 0.000075 |
Nucleus | 0.000036 | 0.000030 | 0.000097 | 0.000230 | 0.000101 | 0.000057 | 0.000227 | 0.000106 | 0.000047 | 0.000053 | 0.000156 | 0.000188 |
Peroxisomes | 0.000015 | 0.000043 | 0.000002 | 0.000078 | 0.000025 | 0.000075 | 0.000014 | 0.000097 | 0.000017 | 0.000071 | 0.000008 | 0.000084 |
Vacuole | 0.002601 | 0.001971 | 0.009956 | 0.016425 | 0.005459 | 0.001693 | 0.012756 | 0.017447 | 0.003087 | 0.001728 | 0.011239 | 0.016775 |
Vacuole Periphery | 0.002317 | 0.001439 | 0.002718 | 0.006038 | 0.004727 | 0.000385 | 0.000766 | 0.004658 | 0.002727 | 0.000516 | 0.001824 | 0.005566 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.56 | 0.86 | -2.38 | -1.72 | -2.94 | 1.50 | -0.58 | 1.72 | 1.85 | 2.80 | 0.76 | -1.32 | -1.44 | -2.24 | -0.08 |
Bud Neck | 0.43 | -4.06 | -4.00 | -4.11 | -1.06 | 2.98 | -3.09 | 1.29 | -1.85 | 3.79 | 1.84 | -4.78 | -3.31 | -4.23 | 3.00 |
Bud Site | -1.38 | -0.51 | -4.97 | -0.48 | -4.55 | 0.74 | -2.59 | -0.47 | -1.18 | 2.23 | -1.55 | -3.10 | -4.84 | -3.41 | -0.59 |
Cell Periphery | -0.59 | -5.38 | -12.31 | -11.69 | -7.48 | 2.03 | 0.24 | -3.79 | -11.10 | -6.84 | 2.02 | -4.08 | -13.17 | -16.63 | -9.72 |
Cytoplasm | -1.41 | -0.90 | -2.75 | 0.10 | -1.52 | -3.82 | -2.57 | -2.51 | 1.60 | 0.53 | -3.77 | -1.87 | -3.15 | -0.07 | -1.35 |
Cytoplasmic Foci | -0.98 | 2.53 | -1.81 | -0.34 | -2.76 | -0.47 | 1.03 | 0.82 | 1.77 | -0.68 | -1.60 | 2.16 | -1.47 | 0.23 | -2.73 |
Eisosomes | -1.76 | -1.64 | -5.46 | -3.87 | -0.87 | 2.31 | 2.09 | 2.12 | -0.96 | 0.07 | 0.76 | -0.22 | -1.17 | -1.87 | -0.91 |
Endoplasmic Reticulum | -0.31 | 3.21 | 10.57 | 9.09 | 7.01 | -3.08 | 2.95 | 3.76 | 11.83 | 0.39 | -2.34 | 5.95 | 11.91 | 16.92 | 4.33 |
Endosome | 1.97 | -0.18 | -1.91 | -5.16 | -2.00 | 1.34 | -2.54 | 1.28 | -0.22 | 5.60 | 0.21 | -3.99 | -0.96 | -1.66 | 3.94 |
Golgi | 0.78 | 3.01 | -2.13 | -3.02 | -8.61 | 2.59 | 2.20 | -1.23 | -7.94 | -7.06 | 4.29 | 3.72 | -2.79 | -12.22 | -11.25 |
Lipid Particles | 2.04 | -1.04 | -8.05 | -10.16 | -6.27 | -2.76 | -5.35 | -8.30 | -7.19 | 0.59 | -0.46 | -4.76 | -10.47 | -10.94 | -2.58 |
Mitochondria | 1.08 | 1.47 | -2.18 | -2.60 | -2.71 | 1.89 | 2.02 | 2.05 | 0.91 | 0.07 | 1.79 | 2.38 | -1.38 | -2.42 | -2.68 |
Mitotic Spindle | 0.80 | 0.64 | -0.86 | -2.02 | -1.84 | -0.61 | -1.22 | 0.35 | 0.76 | 1.27 | 0.24 | -0.37 | -0.60 | -1.01 | -0.20 |
None | -0.98 | 3.30 | -2.39 | 0.52 | -3.02 | -2.16 | 0.53 | -3.90 | 1.86 | -4.21 | -2.21 | 3.29 | -2.86 | 1.67 | -3.66 |
Nuclear Periphery | 0.67 | -5.44 | -5.83 | -7.15 | 3.97 | 3.36 | -0.72 | -0.55 | -9.92 | 0.35 | 2.97 | -6.33 | -5.88 | -13.58 | 4.01 |
Nuclear Periphery Foci | 1.63 | -2.76 | -1.24 | -3.86 | 2.06 | 2.08 | -3.55 | 0.16 | -2.11 | 3.70 | 0.84 | -4.75 | -1.29 | -2.87 | 4.21 |
Nucleolus | 0.51 | -0.97 | -1.38 | -1.40 | -1.35 | 3.51 | 2.76 | 1.71 | -6.27 | -2.91 | 3.01 | 0.56 | -1.37 | -1.49 | -1.39 |
Nucleus | 0.50 | -1.11 | -3.63 | -3.72 | -1.92 | 1.62 | -1.67 | -0.34 | -2.02 | 1.59 | -0.60 | -2.27 | -3.87 | -3.64 | -0.12 |
Peroxisomes | -1.06 | 2.75 | -2.90 | -1.04 | -3.54 | -1.51 | 0.85 | -1.82 | -0.44 | -2.15 | -1.97 | 1.80 | -3.48 | -0.41 | -3.97 |
Vacuole | 0.78 | -5.64 | -9.07 | -9.41 | -3.63 | 3.10 | -3.82 | -5.77 | -8.71 | -1.55 | 2.23 | -7.28 | -11.22 | -13.07 | -3.48 |
Vacuole Periphery | 1.36 | -0.51 | -3.99 | -5.00 | -3.40 | 2.47 | 2.21 | 0.02 | -6.70 | -5.62 | 4.39 | 1.47 | -3.78 | -8.63 | -5.60 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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