Standard name | |
---|---|
Human Ortholog | |
Description | Anchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 18 | 20 | 11 | 11 | 6 | 15 | – | 10 | 8 | 9 | 7 | 7 | 5 | 1 | 2 | 0 | 0 | 6 | 28 | 17 | 43 | 59 | 69 |
Bud | 4 | 8 | 5 | 7 | 15 | 10 | 31 | – | 27 | 31 | 24 | 24 | 10 | 4 | 1 | 0 | 1 | 1 | 14 | 38 | 26 | 24 | 25 | 19 |
Bud Neck | 1 | 1 | 19 | 13 | 9 | 11 | 12 | – | 11 | 11 | 2 | 15 | 1 | 2 | 0 | 7 | 3 | 7 | 7 | 13 | 10 | 8 | 6 | 8 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 0 | – | 2 | 7 | 6 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 8 | 13 | 7 | 4 | 4 | 2 | – | 3 | 6 | 4 | 8 | 6 | 1 | 11 | 10 | 3 | 6 | 1 | 5 | 7 | 31 | 25 | 18 |
Cytoplasm | 146 | 255 | 351 | 270 | 234 | 304 | 306 | – | 319 | 304 | 260 | 350 | 607 | 600 | 639 | 527 | 404 | 325 | 90 | 127 | 163 | 21 | 20 | 18 |
Endoplasmic Reticulum | 17 | 28 | 27 | 12 | 9 | 4 | 14 | – | 12 | 6 | 3 | 4 | 13 | 14 | 17 | 48 | 18 | 30 | 37 | 45 | 38 | 190 | 234 | 247 |
Endosome | 7 | 13 | 1 | 8 | 14 | 25 | 15 | – | 14 | 4 | 7 | 13 | 12 | 12 | 2 | 1 | 3 | 4 | 6 | 15 | 12 | 3 | 6 | 8 |
Golgi | 4 | 2 | 4 | 12 | 5 | 7 | 8 | – | 1 | 4 | 1 | 3 | 0 | 5 | 4 | 1 | 0 | 0 | 2 | 4 | 2 | 40 | 97 | 110 |
Mitochondria | 18 | 15 | 10 | 25 | 51 | 116 | 146 | – | 227 | 215 | 200 | 170 | 16 | 11 | 8 | 24 | 4 | 7 | 44 | 64 | 149 | 40 | 52 | 54 |
Nucleus | 0 | 0 | 1 | 1 | 2 | 2 | 4 | – | 6 | 4 | 10 | 12 | 0 | 2 | 2 | 7 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 4 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 4 | 9 | – | 8 | 4 | 3 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 3 | 0 | 2 | 5 | 2 | 0 | – | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 8 | 2 | 12 | 20 | 17 | 32 | – | 28 | 17 | 15 | 54 | 3 | 1 | 2 | 3 | 1 | 2 | 2 | 10 | 13 | 2 | 1 | 2 |
Vac/Vac Membrane | 12 | 8 | 10 | 12 | 36 | 34 | 33 | – | 38 | 40 | 55 | 67 | 7 | 16 | 11 | 8 | 12 | 2 | 14 | 30 | 30 | 9 | 17 | 20 |
Unique Cell Count | 196 | 337 | 421 | 347 | 362 | 476 | 507 | 586 | 551 | 508 | 628 | 665 | 654 | 681 | 556 | 427 | 338 | 233 | 391 | 484 | 423 | 556 | 592 | |
Labelled Cell Count | 218 | 367 | 463 | 393 | 415 | 549 | 627 | 710 | 663 | 601 | 734 | 686 | 675 | 702 | 641 | 452 | 385 | 233 | 391 | 484 | 423 | 556 | 592 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.8 | 5.7 | 5.6 | 6.5 | 5.1 | 5.7 | – | 4.5 | 4.4 | 4.4 | 4.9 | 4.7 | 4.7 | 4.9 | 5.5 | 5.6 | 5.2 | 47.1 | 56.5 | 67.5 |
Std Deviation (1e-4) | 3.2 | 3.5 | 2.1 | 2.9 | 4.5 | 4.3 | 3.9 | – | 2.4 | 2.0 | 2.2 | 2.8 | 2.6 | 2.5 | 2.4 | 1.4 | 1.3 | 1.2 | 19.4 | 22.8 | 22.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.18 | -0.16 | -0.01 | – | -0.35 | -0.39 | -0.38 | -0.23 | -0.27 | -0.27 | -0.23 | -0.06 | -0.03 | -0.15 | 3.05 | 3.31 | 3.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.1 | -1.2 | -3.1 | -1.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.6 | -4.6 | -4.1 |
Bud | 0.9 | 2.6 | 1.1 | 3.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -0.5 | -1.5 | -1.8 | -1.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.5 | -1.8 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -1.9 | -2.5 | -3.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | -2.6 | -1.2 |
Cytoplasm | -2.0 | -6.0 | -6.6 | -7.7 | – | -9.6 | -9.3 | -10.3 | -9.3 | 3.9 | 4.2 | 5.6 | 5.9 | 5.2 | 5.6 |
Endoplasmic Reticulum | -1.9 | -2.6 | -4.6 | -2.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | -1.4 | 1.3 |
Endosome | 0 | 3.7 | 4.5 | 3.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.4 | 0 | 0.7 | 0.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 6.1 | 9.5 | 10.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | -1.6 | -0.3 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3.1 | 4.3 | 3.2 | 4.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.9 | 4.5 | 3.3 | 3.0 | – | 3.0 | 3.4 | 5.0 | 5.0 | -1.7 | 0.1 | -0.9 | -1.1 | 0.4 | -2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.577 | 2.6487 | 3.8679 | -0.1491 | 2.3686 | 2.9202 | 7.9297 | 8.8907 | 6.8316 | 1.1451 | 4.3464 | 6.2312 | 7.0281 | 4.3243 | 5.1812 | 2.1797 | 4.5956 | 4.47 |
Actin | 0.0138 | 0.0144 | 0.0126 | 0.0043 | 0.0313 | 0.0165 | 0.0565 | 0.0179 | 0.0459 | 0.0117 | 0.017 | 0.0287 | 0.0091 | 0.0144 | 0.0166 | 0.0029 | 0.0315 | 0.0254 |
Bud | 0.0113 | 0.0287 | 0.0217 | 0.0126 | 0.0139 | 0.014 | 0.0209 | 0.0376 | 0.0286 | 0.045 | 0.0362 | 0.0325 | 0.0149 | 0.0512 | 0.0252 | 0.0255 | 0.0256 | 0.0161 |
Bud Neck | 0.0037 | 0.0026 | 0.0048 | 0.0055 | 0.0084 | 0.0268 | 0.0061 | 0.0036 | 0.0086 | 0.0028 | 0.007 | 0.0249 | 0.0054 | 0.0025 | 0.0047 | 0.0078 | 0.0176 | 0.0383 |
Bud Periphery | 0.0344 | 0.049 | 0.0344 | 0.1207 | 0.1442 | 0.0559 | 0.0588 | 0.0491 | 0.0874 | 0.1226 | 0.0573 | 0.1076 | 0.0395 | 0.0593 | 0.0692 | 0.0891 | 0.091 | 0.0548 |
Bud Site | 0.0275 | 0.1034 | 0.0578 | 0.0062 | 0.0026 | 0.0073 | 0.0186 | 0.1516 | 0.0727 | 0.0128 | 0.014 | 0.0078 | 0.0381 | 0.1471 | 0.0637 | 0.0272 | 0.0561 | 0.0043 |
Cell Periphery | 0.0283 | 0.0188 | 0.0258 | 0.0283 | 0.0107 | 0.0122 | 0.0466 | 0.0445 | 0.0361 | 0.0069 | 0.0207 | 0.0256 | 0.0312 | 0.0169 | 0.0259 | 0.0144 | 0.0213 | 0.0125 |
Cytoplasm | 0.2643 | 0.2902 | 0.2529 | 0.3751 | 0.3772 | 0.2688 | 0.2833 | 0.2389 | 0.3197 | 0.2343 | 0.2342 | 0.288 | 0.2663 | 0.2392 | 0.244 | 0.3113 | 0.1706 | 0.2647 |
Cytoplasmic Foci | 0.0282 | 0.0146 | 0.0174 | 0.015 | 0.0304 | 0.0253 | 0.0296 | 0.0226 | 0.0151 | 0.0178 | 0.0331 | 0.0402 | 0.0333 | 0.0219 | 0.0297 | 0.0278 | 0.0557 | 0.0301 |
Eisosomes | 0.0007 | 0.0006 | 0.0007 | 0.0004 | 0.0006 | 0.0003 | 0.0011 | 0.0012 | 0.001 | 0.0003 | 0.0008 | 0.0008 | 0.0006 | 0.0006 | 0.0008 | 0.0001 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0999 | 0.043 | 0.126 | 0.084 | 0.0345 | 0.1164 | 0.1679 | 0.0836 | 0.0983 | 0.1055 | 0.1398 | 0.1392 | 0.138 | 0.0498 | 0.0814 | 0.0643 | 0.0721 | 0.0988 |
Endosome | 0.0361 | 0.0164 | 0.0333 | 0.0302 | 0.0238 | 0.0311 | 0.0204 | 0.0315 | 0.0118 | 0.0145 | 0.0391 | 0.0301 | 0.0444 | 0.0276 | 0.0422 | 0.0331 | 0.0547 | 0.0576 |
Golgi | 0.0071 | 0.0047 | 0.0117 | 0.0026 | 0.0073 | 0.0108 | 0.0112 | 0.0079 | 0.0056 | 0.0017 | 0.0214 | 0.0132 | 0.0069 | 0.0049 | 0.013 | 0.0035 | 0.0292 | 0.0217 |
Lipid Particles | 0.0205 | 0.0099 | 0.0104 | 0.0009 | 0.0031 | 0.0128 | 0.0124 | 0.0075 | 0.008 | 0.0021 | 0.0095 | 0.0069 | 0.0147 | 0.0068 | 0.0082 | 0.0013 | 0.0161 | 0.008 |
Mitochondria | 0.0164 | 0.0113 | 0.0136 | 0.0068 | 0.0099 | 0.0035 | 0.0061 | 0.0095 | 0.0032 | 0.0052 | 0.0073 | 0.0085 | 0.0075 | 0.0079 | 0.0157 | 0.0088 | 0.0165 | 0.0288 |
None | 0.3572 | 0.3451 | 0.3241 | 0.2431 | 0.2899 | 0.3522 | 0.1821 | 0.2153 | 0.2191 | 0.3582 | 0.3136 | 0.1882 | 0.2497 | 0.2748 | 0.287 | 0.3199 | 0.2766 | 0.2814 |
Nuclear Periphery | 0.0079 | 0.0052 | 0.0102 | 0.016 | 0.0019 | 0.0115 | 0.0238 | 0.0119 | 0.0079 | 0.0125 | 0.0062 | 0.0116 | 0.0187 | 0.0088 | 0.0117 | 0.011 | 0.0094 | 0.0091 |
Nucleolus | 0.0013 | 0.0021 | 0.0013 | 0.0007 | 0.0002 | 0.0006 | 0.0014 | 0.0026 | 0.0012 | 0.001 | 0.0011 | 0.0011 | 0.0042 | 0.004 | 0.0054 | 0.0014 | 0.0021 | 0.0015 |
Nucleus | 0.0035 | 0.0086 | 0.0091 | 0.0091 | 0.0011 | 0.0049 | 0.0072 | 0.015 | 0.0061 | 0.0068 | 0.0104 | 0.008 | 0.0104 | 0.0183 | 0.0124 | 0.0172 | 0.0172 | 0.007 |
Peroxisomes | 0.0013 | 0.0006 | 0.0019 | 0.0013 | 0.0014 | 0.0005 | 0.0044 | 0.0013 | 0.0013 | 0.0004 | 0.0022 | 0.0007 | 0.0009 | 0.0013 | 0.0014 | 0.002 | 0.0027 | 0.0011 |
Punctate Nuclear | 0.0017 | 0.0049 | 0.0031 | 0.0008 | 0.0006 | 0.0015 | 0.0071 | 0.0072 | 0.0019 | 0.0029 | 0.0026 | 0.0046 | 0.0046 | 0.0122 | 0.0049 | 0.0011 | 0.009 | 0.0107 |
Vacuole | 0.0317 | 0.0245 | 0.0246 | 0.0323 | 0.0056 | 0.0239 | 0.0306 | 0.037 | 0.0184 | 0.0301 | 0.0246 | 0.0282 | 0.0572 | 0.0282 | 0.0327 | 0.0268 | 0.0215 | 0.0234 |
Vacuole Periphery | 0.0035 | 0.0015 | 0.0027 | 0.0041 | 0.0012 | 0.003 | 0.0039 | 0.0028 | 0.002 | 0.005 | 0.002 | 0.0038 | 0.0044 | 0.0024 | 0.0041 | 0.0035 | 0.0032 | 0.0043 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.5026 | 12.2278 | 10.0817 | 24.1456 | 20.0338 | 41.5193 | 29.6496 | 24.7246 | 13.7127 | 29.4659 |
Translational Efficiency | 1.4752 | 1.8824 | 1.4076 | 0.499 | 1.5297 | 1.9669 | 1.3757 | 1.4991 | 2.7012 | 1.3107 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
563 | 965 | 316 | 444 | 1629 | 1742 | 1964 | 811 | 2192 | 2707 | 2280 | 1255 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 578.09 | 714.84 | 895.62 | 732.79 | 632.36 | 702.58 | 830.76 | 936.01 | 618.42 | 706.95 | 839.75 | 864.11 |
Standard Deviation | 58.81 | 171.28 | 240.34 | 103.81 | 108.68 | 158.97 | 150.50 | 138.53 | 101.13 | 163.57 | 167.39 | 160.18 |
Intensity Change Log 2 | — | 0.306326 | 0.631593 | 0.342106 | — | 0.151916 | 0.393686 | 0.565778 | — | 0.227724 | 0.512207 | 0.463268 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000100 | 0.003207 | 0.004560 | 0.000263 | 0.000461 | 0.002805 | 0.000828 | 0.000229 | 0.000369 | 0.002948 | 0.001345 | 0.000241 |
Bud Neck | 0.033667 | 0.046376 | 0.015357 | 0.009146 | 0.040062 | 0.071508 | 0.013934 | 0.022961 | 0.038420 | 0.062549 | 0.014131 | 0.018073 |
Bud Site | 0.006693 | 0.064233 | 0.049182 | 0.014130 | 0.027159 | 0.095225 | 0.031906 | 0.048296 | 0.021902 | 0.084177 | 0.034301 | 0.036208 |
Cell Periphery | 0.000300 | 0.002681 | 0.004605 | 0.000391 | 0.000552 | 0.001970 | 0.001201 | 0.000341 | 0.000488 | 0.002223 | 0.001673 | 0.000359 |
Cytoplasm | 0.427149* | 0.277627* | 0.288993* | 0.375435* | 0.391771* | 0.274264* | 0.496386* | 0.571058* | 0.400858* | 0.275463* | 0.467642* | 0.501849* |
Cytoplasmic Foci | 0.272593* | 0.287059* | 0.057834 | 0.052103 | 0.239185* | 0.266019* | 0.017255 | 0.004510 | 0.247766* | 0.273520* | 0.022879 | 0.021348 |
Eisosomes | 0.000557 | 0.000407 | 0.000164 | 0.000143 | 0.000296 | 0.000460 | 0.000042 | 0.000029 | 0.000363 | 0.000441 | 0.000059 | 0.000069 |
Endoplasmic Reticulum | 0.001775 | 0.014161 | 0.032204 | 0.000968 | 0.006055 | 0.009796 | 0.005339 | 0.000584 | 0.004956 | 0.011352 | 0.009063 | 0.000720 |
Endosome | 0.012070 | 0.044573 | 0.018387 | 0.011320 | 0.020985 | 0.044134 | 0.003362 | 0.002110 | 0.018695 | 0.044291 | 0.005444 | 0.005369 |
Golgi | 0.003520 | 0.019873 | 0.009015 | 0.002435 | 0.005244 | 0.025298 | 0.000430 | 0.000804 | 0.004802 | 0.023364 | 0.001620 | 0.001381 |
Lipid Particles | 0.021190 | 0.017943 | 0.008217 | 0.003720 | 0.016380 | 0.014022 | 0.001476 | 0.000293 | 0.017615 | 0.015420 | 0.002411 | 0.001506 |
Mitochondria | 0.007489 | 0.032312 | 0.000432 | 0.002669 | 0.009389 | 0.055122 | 0.001062 | 0.003497 | 0.008901 | 0.046990 | 0.000975 | 0.003204 |
Mitotic Spindle | 0.000958 | 0.005846 | 0.004805 | 0.010498 | 0.002278 | 0.005994 | 0.005712 | 0.018227 | 0.001939 | 0.005941 | 0.005586 | 0.015493 |
None | 0.002407 | 0.010147 | 0.003147 | 0.010484 | 0.006424 | 0.003341 | 0.003672 | 0.008723 | 0.005392 | 0.005767 | 0.003599 | 0.009346 |
Nuclear Periphery | 0.001083 | 0.000484 | 0.003142 | 0.001243 | 0.000656 | 0.000282 | 0.001383 | 0.000719 | 0.000766 | 0.000354 | 0.001627 | 0.000905 |
Nuclear Periphery Foci | 0.000204 | 0.001038 | 0.007701 | 0.001760 | 0.000606 | 0.000492 | 0.000832 | 0.000149 | 0.000503 | 0.000687 | 0.001784 | 0.000719 |
Nucleolus | 0.000959 | 0.003334 | 0.000831 | 0.001246 | 0.002231 | 0.001080 | 0.000360 | 0.001219 | 0.001904 | 0.001883 | 0.000425 | 0.001229 |
Nucleus | 0.162072* | 0.061482 | 0.366861* | 0.423506* | 0.164087* | 0.040163 | 0.355816* | 0.273121* | 0.163569* | 0.047763 | 0.357347* | 0.326325* |
Peroxisomes | 0.008123 | 0.035053 | 0.002342 | 0.002159 | 0.010227 | 0.030103 | 0.000650 | 0.000455 | 0.009686 | 0.031868 | 0.000885 | 0.001058 |
Vacuole | 0.036052 | 0.066329 | 0.120529 | 0.074124 | 0.053788 | 0.051712 | 0.057917 | 0.041267 | 0.049233 | 0.056923 | 0.066595 | 0.052891 |
Vacuole Periphery | 0.001040 | 0.005835 | 0.001689 | 0.002256 | 0.002163 | 0.006211 | 0.000435 | 0.001408 | 0.001874 | 0.006077 | 0.000609 | 0.001708 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.26 | -2.88 | -2.50 | 4.01 | 2.74 | -6.07 | -1.39 | 3.21 | 6.82 | 3.07 | -7.17 | -3.29 | 2.36 | 7.62 | 3.99 |
Bud Neck | -2.46 | 3.93 | 5.38 | 9.99 | 1.30 | -7.99 | 10.17 | 6.84 | 14.58 | -3.11 | -7.78 | 10.91 | 9.05 | 17.51 | -1.62 |
Bud Site | -10.87 | -5.29 | -1.84 | 8.51 | 3.96 | -12.83 | 0.08 | -1.61 | 10.10 | -1.71 | -15.47 | -2.25 | -1.54 | 12.85 | 0.47 |
Cell Periphery | -2.25 | -1.26 | -0.60 | 2.22 | 1.25 | -3.67 | -1.15 | 2.85 | 4.61 | 2.07 | -3.84 | -1.77 | 2.61 | 4.37 | 2.24 |
Cytoplasm | 11.72 | 9.07 | 5.35 | -4.17 | -3.45 | 12.86 | -7.76 | -11.02 | -21.03 | -4.90 | 16.85 | -4.47 | -5.78 | -18.91 | -1.97 |
Cytoplasmic Foci | -1.24 | 23.59 | 24.95 | 28.93 | 0.63 | -3.42 | 40.64 | 43.45 | 46.55 | 10.76 | -3.99 | 48.55 | 47.83 | 53.97 | 0.91 |
Eisosomes | 1.25 | 3.60 | 3.79 | 4.57 | 0.81 | -4.09 | 9.19 | 9.79 | 14.39 | 2.88 | -1.76 | 8.72 | 8.45 | 13.22 | -1.79 |
Endoplasmic Reticulum | -4.12 | -4.33 | 0.04 | 4.30 | 4.37 | -1.89 | 0.75 | 4.21 | 5.83 | 5.10 | -3.71 | -2.13 | 3.98 | 7.16 | 6.35 |
Endosome | -12.24 | -1.60 | 1.05 | 11.37 | 2.20 | -9.07 | 11.17 | 11.93 | 20.86 | 2.78 | -13.05 | 10.72 | 9.94 | 23.30 | 0.53 |
Golgi | -8.62 | -1.14 | 1.50 | 9.46 | 1.72 | -12.02 | 6.66 | 6.11 | 16.22 | -0.61 | -14.46 | 5.02 | 5.72 | 18.72 | 0.52 |
Lipid Particles | 1.09 | 5.57 | 7.73 | 7.78 | 3.45 | 1.45 | 12.27 | 13.28 | 13.82 | 6.13 | 1.49 | 14.17 | 14.93 | 15.19 | 2.69 |
Mitochondria | -7.23 | 4.75 | 3.27 | 9.58 | -2.71 | -14.23 | 7.98 | 5.54 | 16.90 | -2.46 | -15.78 | 9.16 | 6.54 | 19.34 | -3.17 |
Mitotic Spindle | -3.38 | -1.73 | -2.58 | -0.99 | -1.29 | -3.49 | -2.75 | -5.39 | -3.98 | -4.19 | -4.61 | -3.36 | -5.87 | -3.91 | -4.15 |
None | -4.08 | -0.62 | -2.53 | 0.58 | -2.33 | 2.78 | 2.93 | -1.70 | -6.66 | -7.87 | -0.52 | 2.59 | -2.59 | -2.14 | -5.34 |
Nuclear Periphery | 1.08 | -2.23 | -0.96 | -4.83 | 1.91 | 4.50 | -7.94 | -5.36 | -9.62 | 2.64 | 2.73 | -5.62 | -3.69 | -9.87 | 2.74 |
Nuclear Periphery Foci | -3.00 | -6.80 | -1.72 | -0.78 | 4.01 | 0.82 | -1.59 | 4.34 | 4.49 | 5.66 | -1.45 | -6.29 | -0.69 | -0.12 | 2.73 |
Nucleolus | -4.10 | 0.34 | -0.94 | 3.60 | -1.06 | 4.10 | 5.64 | 3.83 | -0.20 | -3.05 | -0.06 | 5.62 | 3.43 | 3.20 | -3.33 |
Nucleus | 13.53 | -11.58 | -16.60 | -24.70 | -1.38 | 25.78 | -27.97 | -16.44 | -31.12 | 4.87 | 28.26 | -30.83 | -22.98 | -39.35 | 2.62 |
Peroxisomes | -9.30 | 4.73 | 4.66 | 12.27 | 0.45 | -11.37 | 12.74 | 13.05 | 18.90 | 1.85 | -14.55 | 13.79 | 12.92 | 22.16 | -0.11 |
Vacuole | -7.22 | -10.49 | -9.62 | -6.42 | 1.02 | 0.87 | -4.86 | -0.77 | -1.40 | 3.12 | -2.86 | -9.46 | -7.40 | -5.80 | -0.21 |
Vacuole Periphery | -7.83 | -0.68 | -1.09 | 4.52 | -0.45 | -6.77 | 5.77 | 3.15 | 9.25 | -2.44 | -9.21 | 5.52 | 1.72 | 9.98 | -2.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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