Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 12 | 12 | 15 | 40 | 35 | 65 | 28 | 45 | 58 | 22 | 37 | 19 | 26 | 12 | 5 | 5 | 5 | 4 | 15 | 7 | 13 | 21 | 39 |
Bud | 22 | 17 | 39 | 36 | 64 | 89 | 124 | 110 | 89 | 110 | 84 | 81 | 22 | 22 | 13 | 10 | 8 | 8 | 2 | 7 | 7 | 7 | 9 | 7 |
Bud Neck | 0 | 1 | 3 | 5 | 0 | 3 | 2 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 3 | 0 | 2 | 4 | 4 | 6 | 5 | 2 |
Bud Site | 27 | 14 | 18 | 10 | 19 | 40 | 51 | 22 | 34 | 27 | 28 | 34 | 8 | 4 | 3 | 8 | 7 | 12 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 4 | 6 | 7 | 3 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 3 | 9 | 3 | 20 | 5 | 15 | 22 | 8 | 15 | 7 | 7 | 6 | 12 | 10 | 12 | 4 | 13 | 0 | 0 | 0 | 2 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 4 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
Endosome | 11 | 21 | 24 | 30 | 104 | 60 | 72 | 92 | 47 | 53 | 32 | 32 | 15 | 25 | 43 | 46 | 41 | 31 | 25 | 32 | 21 | 27 | 52 | 25 |
Golgi | 0 | 0 | 0 | 0 | 10 | 3 | 0 | 2 | 2 | 0 | 0 | 1 | 3 | 8 | 4 | 10 | 8 | 1 | 4 | 7 | 5 | 6 | 6 | 10 |
Mitochondria | 101 | 116 | 58 | 71 | 46 | 105 | 187 | 121 | 175 | 188 | 271 | 223 | 24 | 27 | 36 | 20 | 26 | 16 | 2 | 8 | 1 | 6 | 10 | 5 |
Nucleus | 2 | 2 | 1 | 1 | 0 | 2 | 2 | 6 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 10 | 18 | 4 | 10 | 20 | 27 | 32 | 22 | 23 | 26 | 23 | 29 | 10 | 10 | 10 | 19 | 18 | 15 | 1 | 0 | 0 | 2 | 0 | 0 |
Peroxisomes | 180 | 135 | 249 | 213 | 244 | 333 | 395 | 397 | 298 | 332 | 224 | 292 | 154 | 175 | 225 | 146 | 142 | 109 | 200 | 156 | 259 | 128 | 209 | 293 |
SpindlePole | 92 | 44 | 181 | 110 | 122 | 151 | 200 | 213 | 142 | 122 | 85 | 106 | 91 | 102 | 122 | 96 | 104 | 75 | 34 | 35 | 47 | 48 | 49 | 21 |
Vac/Vac Membrane | 0 | 2 | 0 | 3 | 4 | 2 | 5 | 18 | 6 | 10 | 8 | 9 | 1 | 1 | 3 | 3 | 3 | 5 | 0 | 2 | 0 | 0 | 2 | 3 |
Unique Cell Count | 267 | 245 | 358 | 292 | 422 | 507 | 693 | 650 | 509 | 567 | 505 | 537 | 245 | 274 | 324 | 255 | 253 | 188 | 281 | 277 | 358 | 257 | 375 | 421 |
Labelled Cell Count | 450 | 387 | 598 | 507 | 697 | 860 | 1153 | 1059 | 878 | 953 | 793 | 860 | 360 | 413 | 486 | 384 | 373 | 294 | 281 | 277 | 358 | 257 | 375 | 421 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 7.1 | 6.0 | 6.7 | 9.5 | 8.1 | 7.3 | 7.7 | 7.6 | 7.9 | 7.1 | 7.5 | 9.1 | 9.7 | 10.3 | 10.5 | 10.0 | 9.3 | 8.6 | 8.3 | 8.5 |
Std Deviation (1e-4) | 1.0 | 1.5 | 1.4 | 1.5 | 2.5 | 2.2 | 1.9 | 2.1 | 2.2 | 2.4 | 2.1 | 2.4 | 2.1 | 2.4 | 3.1 | 3.0 | 3.1 | 2.4 | 2.7 | 2.2 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.65 | 0.43 | 0.28 | 0.36 | 0.33 | 0.39 | 0.24 | 0.31 | 0.6 | 0.68 | 0.77 | 0.8 | 0.74 | 0.63 | 0.52 | 0.47 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | 3.4 | 2.3 | 3.6 | 0.7 | 3.2 | 3.9 | 0.7 | 2.3 | 2.4 | 3.2 | 0.2 | -1.0 | -1.0 | -0.4 |
Bud | 0.6 | 1.8 | 2.7 | 3.0 | 2.6 | 2.7 | 3.4 | 2.4 | 1.8 | -0.8 | -1.2 | -3.4 | -3.1 | -3.5 | -2.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.0 | -0.3 | 1.7 | 1.4 | -1.3 | 1.0 | -0.2 | 0.3 | 0.8 | -1.0 | -2.4 | -3.1 | -1.1 | -1.4 | 0.7 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.4 | 1.6 | -1.8 | -0.4 | 0.8 | -1.0 | 0.1 | -1.2 | -1.3 | -0.1 | 1.3 | 0.5 | 1.5 | -0.8 | 2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.6 | 6.7 | 2.5 | 2.0 | 3.5 | 1.3 | 1.4 | -0.2 | -0.5 | -0.3 | 1.1 | 2.9 | 4.3 | 3.8 | 3.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | -2.2 | 1.7 | 3.9 | 1.0 | 5.9 | 5.7 | 11.2 | 8.0 | -2.3 | -2.3 | -1.9 | -3.1 | -2.1 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.0 | 2.9 | 3.3 | 3.0 | 2.2 | 2.8 | 2.9 | 2.9 | 3.3 | 2.4 | 2.1 | 1.8 | 4.1 | 3.9 | 4.2 |
Peroxisomes | 0.9 | -3.4 | -1.2 | -4.0 | -2.7 | -3.3 | -3.4 | -7.3 | -4.5 | -1.7 | -1.5 | 0 | -3.1 | -3.4 | -2.7 |
SpindlePole | -3.3 | -6.2 | -6.2 | -6.9 | -5.5 | -6.8 | -9.2 | -10.6 | -9.7 | -3.3 | -3.3 | -3.4 | -3.2 | -2.3 | -2.4 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.8109 | 7.9354 | 7.891 | 7.0558 | 8.3726 | 7.9376 | 3.4306 | 5.1644 | 4.307 | 3.4433 | 4.0376 | 4.4724 | 3.8392 | 5.4795 | 4.8737 | 5.3389 | 4.0066 | 4.6555 |
Actin | 0.1042 | 0.0547 | 0.0957 | 0.017 | 0.0124 | 0.0034 | 0.0375 | 0.046 | 0.0616 | 0.0016 | 0.0296 | 0.0405 | 0.0212 | 0.032 | 0.0256 | 0.0019 | 0.0347 | 0.0353 |
Bud | 0.0004 | 0.0024 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0029 | 0.0026 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0.003 | 0.0012 | 0 | 0.0032 | 0.0003 |
Bud Neck | 0.0023 | 0.008 | 0.0027 | 0.0006 | 0.0007 | 0.0003 | 0.0016 | 0.004 | 0.009 | 0.0001 | 0.0112 | 0.0215 | 0.0004 | 0.0035 | 0.0019 | 0.0048 | 0.0004 | 0.0006 |
Bud Periphery | 0.0005 | 0.0029 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0015 | 0.0019 | 0.0014 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0026 | 0.0012 | 0.0001 | 0.0031 | 0.0004 |
Bud Site | 0.0035 | 0.0376 | 0.0136 | 0.0005 | 0.0003 | 0.0003 | 0.0128 | 0.0458 | 0.0104 | 0.0003 | 0.0022 | 0.001 | 0.0035 | 0.0294 | 0.0045 | 0.0003 | 0.0013 | 0.0008 |
Cell Periphery | 0.0004 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0077 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0068 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0009 | 0.0006 | 0.0002 | 0 | 0.0009 | 0.0001 |
Cytoplasmic Foci | 0.2274 | 0.1935 | 0.1883 | 0.2586 | 0.2947 | 0.2029 | 0.232 | 0.278 | 0.2405 | 0.2376 | 0.3253 | 0.2234 | 0.1996 | 0.1947 | 0.2079 | 0.1471 | 0.343 | 0.2633 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0067 | 0.0001 | 0 | 0 | 0 | 0 | 0.0068 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0 | 0.0012 | 0.0003 |
Endosome | 0.0343 | 0.0123 | 0.0033 | 0.0024 | 0.0097 | 0.003 | 0.0565 | 0.0095 | 0.0118 | 0.0247 | 0.0177 | 0.0111 | 0.0188 | 0.0164 | 0.0048 | 0.0057 | 0.0133 | 0.0053 |
Golgi | 0.0103 | 0.0201 | 0.0103 | 0.0091 | 0.0408 | 0.0077 | 0.0319 | 0.0154 | 0.0235 | 0.0218 | 0.0359 | 0.0182 | 0.0158 | 0.0216 | 0.0191 | 0.0145 | 0.0192 | 0.0133 |
Lipid Particles | 0.0774 | 0.0584 | 0.097 | 0.0484 | 0.1742 | 0.1542 | 0.1245 | 0.0727 | 0.0617 | 0.1857 | 0.2673 | 0.134 | 0.1189 | 0.0636 | 0.058 | 0.2055 | 0.1922 | 0.22 |
Mitochondria | 0.0033 | 0.015 | 0.0233 | 0.0091 | 0.0123 | 0.0104 | 0.0183 | 0.0092 | 0.0205 | 0.0044 | 0.0039 | 0.0049 | 0.0179 | 0.0224 | 0.0222 | 0.0122 | 0.0579 | 0.0231 |
None | 0.0044 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0002 |
Nuclear Periphery | 0.0205 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.01 | 0.0002 | 0 | 0.0015 | 0 | 0 | 0.001 | 0.0001 | 0 | 0 | 0.018 | 0.0013 |
Nucleolus | 0.0007 | 0.0159 | 0.0004 | 0.0001 | 0.0005 | 0.0028 | 0.0011 | 0.0096 | 0.0016 | 0.0008 | 0.0005 | 0.0003 | 0.0006 | 0.0019 | 0.0015 | 0.0004 | 0.0021 | 0.0069 |
Nucleus | 0.0017 | 0.0014 | 0.0001 | 0 | 0 | 0.0006 | 0.0014 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0043 | 0.0005 |
Peroxisomes | 0.4423 | 0.5685 | 0.5501 | 0.6502 | 0.3491 | 0.6013 | 0.4426 | 0.4917 | 0.5395 | 0.5043 | 0.3018 | 0.5419 | 0.5777 | 0.5909 | 0.6478 | 0.6059 | 0.2923 | 0.4216 |
Punctate Nuclear | 0.048 | 0.0048 | 0.0125 | 0.0035 | 0.1043 | 0.0109 | 0.004 | 0.0071 | 0.014 | 0.0142 | 0.0022 | 0.0016 | 0.02 | 0.0145 | 0.0026 | 0.001 | 0.0044 | 0.006 |
Vacuole | 0.0023 | 0.0025 | 0.0001 | 0.0001 | 0.0004 | 0.0007 | 0.0035 | 0.0036 | 0.0007 | 0.0007 | 0.0005 | 0.0002 | 0.0008 | 0.0016 | 0.0003 | 0.0001 | 0.0034 | 0.0002 |
Vacuole Periphery | 0.0011 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.003 | 0.0008 | 0.0003 | 0.0009 | 0.0002 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.0002 | 0.0042 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.7041 | 34.7928 | 16.7815 | 23.253 | 10.7233 | 40.8442 | 53.2082 | 45.1125 | 42.1392 | 36.6253 |
Translational Efficiency | 1.1947 | 1.1773 | 1.6531 | 0.9866 | 1.6034 | 1.5373 | 1.2612 | 1.5586 | 1.4156 | 1.2065 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2210 | 988 | 1661 | 1451 | 1329 | 891 | 1496 | 299 | 3539 | 1879 | 3157 | 1750 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 806.54 | 845.22 | 1615.28 | 1555.23 | 802.94 | 829.44 | 1273.32 | 1494.20 | 805.19 | 837.74 | 1453.24 | 1544.80 |
Standard Deviation | 105.03 | 143.23 | 327.10 | 456.52 | 110.81 | 119.78 | 243.08 | 331.29 | 107.25 | 132.86 | 336.82 | 438.27 |
Intensity Change Log 2 | — | 0.067581 | 1.001966 | 0.947310 | — | 0.046845 | 0.665231 | 0.896009 | — | 0.057274 | 0.843776 | 0.921945 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007562 | 0.013561 | 0.040676 | 0.389045* | 0.019314 | 0.009769 | 0.078067 | 0.471470* | 0.011975 | 0.011763 | 0.058394 | 0.403128* |
Bud Neck | 0.002804 | 0.002960 | 0.006682 | 0.005807 | 0.002787 | 0.003441 | 0.009174 | 0.003365 | 0.002797 | 0.003188 | 0.007863 | 0.005389 |
Bud Site | 0.009090 | 0.011911 | 0.020702 | 0.031512 | 0.010856 | 0.013441 | 0.030594 | 0.018902 | 0.009753 | 0.012637 | 0.025390 | 0.029357 |
Cell Periphery | 0.000118 | 0.000156 | 0.000099 | 0.000199 | 0.000165 | 0.000142 | 0.000231 | 0.000121 | 0.000136 | 0.000149 | 0.000162 | 0.000186 |
Cytoplasm | 0.002887 | 0.005529 | 0.006998 | 0.005918 | 0.003816 | 0.005696 | 0.006009 | 0.004193 | 0.003236 | 0.005609 | 0.006529 | 0.005623 |
Cytoplasmic Foci | 0.157040 | 0.115022 | 0.092486 | 0.056488 | 0.128135 | 0.123703 | 0.093482 | 0.036685 | 0.146185 | 0.119138 | 0.092958 | 0.053105 |
Eisosomes | 0.000151 | 0.000925 | 0.002528 | 0.003696 | 0.000318 | 0.000185 | 0.001550 | 0.002853 | 0.000214 | 0.000574 | 0.002065 | 0.003552 |
Endoplasmic Reticulum | 0.000072 | 0.000206 | 0.000127 | 0.000426 | 0.000136 | 0.000220 | 0.000384 | 0.000312 | 0.000096 | 0.000213 | 0.000249 | 0.000407 |
Endosome | 0.009685 | 0.017904 | 0.005816 | 0.010913 | 0.010923 | 0.026239 | 0.009615 | 0.010416 | 0.010150 | 0.021857 | 0.007616 | 0.010828 |
Golgi | 0.027423 | 0.039091 | 0.020445 | 0.021449 | 0.032552 | 0.035847 | 0.024733 | 0.014072 | 0.029349 | 0.037552 | 0.022477 | 0.020189 |
Lipid Particles | 0.027592 | 0.062187 | 0.024910 | 0.030856 | 0.038245 | 0.057555 | 0.066566 | 0.016081 | 0.031593 | 0.059990 | 0.044650 | 0.028331 |
Mitochondria | 0.003180 | 0.004312 | 0.029111 | 0.012502 | 0.003644 | 0.007384 | 0.008473 | 0.003813 | 0.003354 | 0.005769 | 0.019331 | 0.011018 |
Mitotic Spindle | 0.003973 | 0.009255 | 0.027124 | 0.011982 | 0.006600 | 0.012361 | 0.049156 | 0.011943 | 0.004959 | 0.010728 | 0.037564 | 0.011975 |
None | 0.000111 | 0.000261 | 0.000139 | 0.000117 | 0.000123 | 0.000137 | 0.000194 | 0.000058 | 0.000116 | 0.000202 | 0.000165 | 0.000107 |
Nuclear Periphery | 0.000017 | 0.000058 | 0.000199 | 0.000115 | 0.000027 | 0.000092 | 0.000235 | 0.000044 | 0.000021 | 0.000074 | 0.000216 | 0.000103 |
Nuclear Periphery Foci | 0.000464 | 0.002890 | 0.000461 | 0.000117 | 0.001024 | 0.001374 | 0.001354 | 0.000057 | 0.000674 | 0.002171 | 0.000884 | 0.000107 |
Nucleolus | 0.000250 | 0.000920 | 0.000265 | 0.000073 | 0.000096 | 0.000393 | 0.000429 | 0.000013 | 0.000192 | 0.000670 | 0.000343 | 0.000063 |
Nucleus | 0.000261 | 0.000362 | 0.000055 | 0.000114 | 0.000084 | 0.000528 | 0.000292 | 0.000019 | 0.000195 | 0.000441 | 0.000167 | 0.000098 |
Peroxisomes | 0.747085* | 0.710868* | 0.719481* | 0.416067* | 0.740777* | 0.699401* | 0.617971* | 0.405064* | 0.744716* | 0.705430* | 0.671379* | 0.414187* |
Vacuole | 0.000179 | 0.000980 | 0.001500 | 0.002152 | 0.000322 | 0.001419 | 0.001397 | 0.000456 | 0.000233 | 0.001188 | 0.001451 | 0.001862 |
Vacuole Periphery | 0.000056 | 0.000642 | 0.000196 | 0.000453 | 0.000055 | 0.000674 | 0.000094 | 0.000062 | 0.000055 | 0.000657 | 0.000148 | 0.000386 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.18 | -12.03 | -41.41* | -40.46* | -36.37* | 3.95 | -12.33 | -21.19 | -21.70* | -18.18* | 0.16 | -17.55 | -45.93* | -45.94* | -39.05* |
Bud Neck | -0.23 | -5.49 | -4.63 | -5.22 | 1.26 | -0.83 | -7.65 | -0.68 | 0.05 | 5.83 | -0.73 | -9.12 | -5.11 | -4.58 | 4.35 |
Bud Site | -1.26 | -6.40 | -9.50 | -7.02 | -4.27 | -1.00 | -7.22 | -2.55 | -1.60 | 3.29 | -1.72 | -9.84 | -9.71 | -7.40 | -1.95 |
Cell Periphery | -1.28 | 1.07 | -4.26 | -1.59 | -6.16 | 0.76 | -1.88 | 1.67 | 1.05 | 3.89 | -0.70 | -1.56 | -3.14 | -2.00 | -1.57 |
Cytoplasm | -2.86 | -7.79 | -5.91 | -0.51 | 1.06 | -1.95 | -2.60 | -0.35 | 1.46 | 1.86 | -3.57 | -6.92 | -5.06 | -0.02 | 1.16 |
Cytoplasmic Foci | 5.88 | 10.35 | 17.61 | 9.16 | 6.97 | 0.59 | 5.24 | 12.69 | 11.47 | 9.29 | 5.15 | 11.69 | 20.65 | 13.61 | 10.28 |
Eisosomes | -7.84 | -6.48 | -9.41 | -7.11 | -2.20 | 4.67 | -13.57 | -3.81 | -4.01 | -1.95 | -6.67 | -9.35 | -10.04 | -8.84 | -3.83 |
Endoplasmic Reticulum | -3.21 | -3.26 | -5.87 | -2.08 | -5.01 | -1.57 | -7.17 | -3.22 | -1.25 | 1.36 | -3.63 | -8.10 | -6.13 | -2.67 | -2.94 |
Endosome | -3.97 | 3.97 | -1.06 | 3.19 | -4.43 | -5.67 | 0.87 | 0.21 | 5.13 | -0.37 | -7.00 | 3.07 | -0.70 | 6.15 | -3.19 |
Golgi | -5.65 | 4.50 | 3.48 | 7.71 | -0.52 | -1.40 | 4.21 | 6.80 | 7.69 | 4.20 | -5.41 | 5.89 | 6.13 | 9.88 | 1.53 |
Lipid Particles | -6.45 | 0.92 | -0.93 | 5.56 | -1.73 | -3.40 | -5.76 | 4.76 | 6.95 | 9.77 | -7.33 | -4.64 | 1.07 | 7.54 | 5.02 |
Mitochondria | -1.07 | -11.35 | -6.65 | -5.34 | 6.51 | -2.04 | -4.07 | -0.14 | 1.80 | 3.30 | -2.33 | -11.91 | -6.62 | -3.72 | 5.00 |
Mitotic Spindle | -2.93 | -9.76 | -4.82 | -1.23 | 5.21 | -3.24 | -11.82 | -1.47 | -0.07 | 6.50 | -4.57 | -15.40 | -4.64 | -0.77 | 9.69 |
None | -1.66 | -0.60 | -0.08 | 1.75 | 1.82 | -0.32 | -0.93 | 2.53 | 2.13 | 1.90 | -1.65 | -1.10 | 0.49 | 2.05 | 1.65 |
Nuclear Periphery | -2.43 | -10.20 | -8.50 | -1.38 | 4.09 | -1.57 | -13.15 | -1.82 | 1.14 | 11.25 | -2.71 | -16.23 | -8.62 | -0.76 | 7.66 |
Nuclear Periphery Foci | -2.75 | -0.21 | 3.43 | 3.10 | 2.04 | -0.54 | -0.92 | 1.74 | 3.56 | 3.68 | -2.83 | -1.22 | 2.60 | 4.01 | 4.11 |
Nucleolus | -1.13 | -0.30 | 1.17 | 1.45 | 3.65 | -1.41 | -1.71 | 2.70 | 1.85 | 2.20 | -1.45 | -1.27 | 1.38 | 1.89 | 3.05 |
Nucleus | -0.77 | 1.21 | 0.44 | 1.25 | -1.26 | -1.57 | -1.64 | 2.59 | 1.81 | 2.16 | -1.50 | 0.10 | 0.35 | 1.83 | 0.33 |
Peroxisomes | 3.62 | 3.24 | 32.27* | 23.61 | 27.02* | 3.59 | 11.58 | 16.00 | 13.40 | 9.83 | 5.21 | 10.79 | 36.57* | 28.37 | 26.61* |
Vacuole | -3.06 | -5.78 | -4.87 | -2.37 | -1.48 | -2.00 | -6.92 | -1.15 | 1.76 | 5.53 | -3.26 | -8.69 | -4.83 | -1.46 | -1.23 |
Vacuole Periphery | -1.14 | -3.22 | -2.82 | 0.34 | -1.79 | -1.94 | -2.34 | -0.23 | 1.91 | 1.09 | -1.94 | -3.60 | -2.85 | 0.81 | -2.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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