ORF
Human Ortholog
Description Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.95 0.96 0.97 0.92 0.81 0.72 0.76 0.63 0.68 0.6 0.61 0.99 0.99 1.0 0.96 0.95 0.96 0.77 0.83 0.67 0.85 0.85 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.05 0 0.07 0.17 0.36 0.5 0.38 0.54 0.55 0.63 0.58 0 0 0 0 0 0 0.12 0.08 0.18 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 6 1 0 5
Bud 0 0 0 1 3 4 16 15 14 12 18 24 0 0 0 0 0 0 0 0 1 6 1 10
Bud Neck 0 1 1 1 2 3 4 1 6 1 0 0 1 9 1 2 1 0 0 0 1 2 2 3
Bud Site 0 0 0 0 0 0 4 1 4 5 7 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 0 1 1 0 1 1 1 1 1 2 0 2 5 1 2 0 3 0 1 2
Cytoplasm 216 163 274 193 246 318 322 359 283 337 232 267 394 534 619 195 200 103 193 154 185 278 350 383
Endoplasmic Reticulum 2 0 1 0 1 0 0 1 0 0 0 0 0 2 0 10 11 10 2 1 3 0 0 4
Endosome 0 1 2 0 1 1 0 2 0 0 0 0 0 0 0 2 2 1 5 3 4 2 4 8
Golgi 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 3 1 0 2
Mitochondria 46 8 11 14 46 143 223 180 243 274 243 256 0 0 0 5 5 1 30 14 50 6 9 11
Nucleus 0 0 0 0 1 2 2 1 1 4 2 2 1 1 1 2 1 1 0 0 0 2 5 5
Nuclear Periphery 0 0 1 0 0 1 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 1 1 0 1 3 1 17 13 13 11 14 19 0 0 0 0 0 0 1 2 4 5 17 34
Vac/Vac Membrane 0 0 1 0 1 4 0 4 2 3 4 1 1 0 2 2 3 0 4 2 1 11 11 7
Unique Cell Count 239 171 285 198 267 392 448 475 449 495 387 440 397 537 622 203 210 107 252 185 277 329 413 491
Labelled Cell Count 265 175 294 211 306 479 589 579 568 648 524 578 398 548 624 221 230 118 252 185 277 329 413 491


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.7 3.8 3.7 3.5 3.5 2.7 2.8 2.9 2.5 2.4 2.3 2.3 4.8 4.7 4.5 5.2 5.2 4.9 5.0 4.8 5.3
Std Deviation (1e-4) 0.3 0.5 2.2 1.8 1.6 1.0 1.5 1.5 0.9 0.5 1.4 0.8 1.2 0.9 1.2 1.4 1.9 1.8 1.7 1.1 1.5
Intensity Change (Log2) – – – -0.1 -0.1 -0.44 -0.44 -0.37 -0.58 -0.66 -0.68 -0.72 0.36 0.35 0.28 0.48 0.48 0.4 0.41 0.37 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5072 -0.8557 -1.2849 -1.6206 -1.2814 -1.0267 -0.904 -0.8111 -0.8675 -1.0164 0.1512 -1.023 -1.2869 -1.2188 -1.091 -0.1025 -1.741 -1.3681
Actin 0.0003 0 0.0003 0.0001 0.0002 0.0003 0.0359 0.0001 0.0141 0.017 0.0448 0.007 0.0299 0 0.0275 0.0634 0.0001 0.0081
Bud 0 0.0001 0.0006 0 0 0.0001 0.0032 0.0004 0.0019 0.002 0.0017 0.0003 0.0014 0 0.001 0.0013 0 0.0003
Bud Neck 0.0001 0 0.0001 0 0 0.0003 0.009 0 0.0063 0.0005 0.0025 0.0021 0.0014 0 0.0013 0.0052 0 0.001
Bud Periphery 0 0.0002 0.0002 0 0 0.0001 0.0083 0.0005 0.0039 0.0075 0.0052 0.0009 0.0033 0 0.0015 0.002 0.0001 0.0007
Bud Site 0.0001 0 0.0004 0 0 0 0.0164 0.003 0.005 0.0005 0.0045 0.0003 0.0059 0.0001 0.0056 0.0104 0 0.0011
Cell Periphery 0 0 0 0 0 0 0.0014 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002 0 0.0002 0.0008 0 0.0001
Cytoplasm 0.0123 0.0114 0.0147 0.0058 0.0073 0.0201 0.0224 0.0298 0.0324 0.0418 0.019 0.0679 0.0353 0.0338 0.0552 0.0245 0.0702 0.0819
Cytoplasmic Foci 0.0054 0.001 0.003 0.0008 0.0012 0.0026 0.033 0.003 0.0096 0.0076 0.038 0.0079 0.0262 0.0012 0.0099 0.0133 0.003 0.0105
Eisosomes 0.0005 0.0001 0.0002 0.0001 0.0002 0.0001 0.0008 0.0001 0.0002 0.0002 0.0003 0.0002 0.0005 0.0001 0.0003 0.0007 0.0001 0.0001
Endoplasmic Reticulum 0.0002 0.0002 0.0001 0 0.0002 0.0002 0.0034 0.0004 0.0009 0.0008 0.0043 0.0007 0.0035 0.0002 0.0008 0.0031 0.0004 0.0037
Endosome 0.0005 0.0001 0.0002 0 0.0001 0.0002 0.012 0.0003 0.0061 0.0081 0.0585 0.0088 0.014 0.0001 0.0054 0.0491 0.0002 0.0056
Golgi 0.0001 0 0 0 0 0 0.0048 0 0.0039 0.0086 0.0179 0.004 0.0045 0 0.0051 0.0126 0 0.0015
Lipid Particles 0.0012 0.0001 0.0001 0 0.0002 0.0002 0.0107 0.0002 0.0022 0.0034 0.0154 0.0016 0.0149 0.0001 0.0033 0.0361 0.0003 0.0052
Mitochondria 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0055 0.0002 0.0024 0.015 0.013 0.0099 0.0066 0 0.0052 0.0081 0.0001 0.0016
None 0.9743 0.9862 0.9737 0.9928 0.9891 0.9686 0.7907 0.96 0.8912 0.856 0.7026 0.8793 0.8212 0.9634 0.8671 0.6945 0.9236 0.8567
Nuclear Periphery 0.0002 0.0001 0.0001 0 0.0004 0.0003 0.0084 0.0003 0.0013 0.001 0.0281 0.0008 0.0034 0.0001 0.0008 0.0201 0.0002 0.0062
Nucleolus 0.0004 0 0.0003 0 0.0001 0.0002 0.0011 0.0002 0.0009 0.0004 0.0008 0.0003 0.0006 0 0.0003 0.0026 0.0001 0.0022
Nucleus 0.0006 0.0001 0.0009 0.0001 0.0003 0.0009 0.0042 0.0004 0.0018 0.001 0.0041 0.0012 0.0023 0.0005 0.0013 0.0206 0.0006 0.003
Peroxisomes 0.0003 0 0.0002 0 0.0001 0.0001 0.0169 0.0001 0.0076 0.0239 0.0223 0.0019 0.0157 0 0.003 0.0061 0.0001 0.002
Punctate Nuclear 0.003 0.0001 0.0047 0.0002 0.0005 0.0054 0.0051 0.0005 0.0066 0.0014 0.011 0.004 0.0072 0.0002 0.0045 0.0137 0.0005 0.0065
Vacuole 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0048 0.0004 0.0011 0.0016 0.0027 0.0004 0.0014 0.0001 0.0006 0.0092 0.0001 0.0013
Vacuole Periphery 0 0 0 0 0 0 0.0021 0.0001 0.0003 0.0014 0.003 0.0004 0.0006 0 0.0002 0.0028 0 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.8438 2.5422 1.8864 5.0673 3.0726 4.7371 4.4982 2.3131 5.5844 6.602
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1985 152 1275 1254 1733 1413 1066 1508 3718 1565 2341 2762

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.45 757.92 783.74 729.64 660.33 695.56 836.98 813.05 666.27 701.62 807.98 775.18
Standard Deviation 73.37 79.29 90.70 101.68 72.30 81.45 94.56 103.01 73.09 83.32 96.20 110.51
Intensity Change Log 2 — 0.174766 0.223095 0.119905 — 0.074988 0.342006 0.300157 — 0.126155 0.283279 0.212092

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000498 0.000106 0.000582 0.000085 0.000328 0.000155 0.001167 0.000104 0.000345 0.000128 0.000901
Bud Neck 0.024277 0.069159 0.003451 0.007638 0.008129 0.030155 0.001681 0.006619 0.016750 0.033943 0.002645 0.007082
Bud Site 0.004703 0.012585 0.003990 0.015467 0.001548 0.006719 0.003025 0.030282 0.003232 0.007289 0.003550 0.023556
Cell Periphery 0.000141 0.000202 0.000169 0.000233 0.000157 0.000190 0.000134 0.000183 0.000149 0.000191 0.000153 0.000206
Cytoplasm 0.422496* 0.228551* 0.327825* 0.399140* 0.498942* 0.367294* 0.282530* 0.443883* 0.458128* 0.353818* 0.307199* 0.423569*
Cytoplasmic Foci 0.261907* 0.359335* 0.019095 0.023809 0.175748 0.289154* 0.012980 0.022554 0.221747* 0.295970* 0.016311 0.023124
Eisosomes 0.000189 0.000231 0.000053 0.000060 0.000144 0.000223 0.000050 0.000051 0.000168 0.000224 0.000052 0.000055
Endoplasmic Reticulum 0.000913 0.000683 0.001274 0.002981 0.001020 0.000571 0.001738 0.001818 0.000963 0.000582 0.001485 0.002346
Endosome 0.012577 0.033854 0.002688 0.003558 0.004574 0.023716 0.002105 0.008438 0.008847 0.024701 0.002423 0.006223
Golgi 0.003649 0.005878 0.000071 0.002649 0.001091 0.004604 0.000090 0.004429 0.002457 0.004728 0.000080 0.003621
Lipid Particles 0.012576 0.009142 0.001955 0.001799 0.009613 0.013328 0.001718 0.001476 0.011195 0.012921 0.001847 0.001623
Mitochondria 0.007039 0.007454 0.000703 0.001637 0.003958 0.009023 0.000463 0.007415 0.005603 0.008871 0.000594 0.004792
Mitotic Spindle 0.001886 0.005400 0.003660 0.009864 0.001467 0.003972 0.000859 0.032177 0.001690 0.004111 0.002385 0.022047
None 0.002389 0.003635 0.008831 0.005371 0.003454 0.003393 0.005311 0.006683 0.002886 0.003417 0.007228 0.006087
Nuclear Periphery 0.000524 0.000343 0.001298 0.001772 0.000595 0.000610 0.001567 0.001754 0.000557 0.000584 0.001421 0.001762
Nuclear Periphery Foci 0.000238 0.000515 0.000385 0.001639 0.000227 0.000286 0.000623 0.000938 0.000233 0.000308 0.000493 0.001256
Nucleolus 0.000987 0.001591 0.001035 0.001321 0.000950 0.002406 0.000956 0.000656 0.000970 0.002327 0.000999 0.000958
Nucleus 0.191195* 0.152560* 0.593608* 0.432094* 0.258998* 0.163625* 0.641382* 0.338687* 0.222799* 0.162550* 0.615363* 0.381095*
Peroxisomes 0.008054 0.036967 0.000362 0.000899 0.003027 0.013719 0.001209 0.002304 0.005711 0.015977 0.000748 0.001666
Vacuole 0.043173 0.069897 0.029133 0.084770 0.025678 0.064646 0.041215 0.085957 0.035019 0.065156 0.034635 0.085418
Vacuole Periphery 0.000969 0.001520 0.000307 0.002717 0.000592 0.002039 0.000206 0.002531 0.000793 0.001989 0.000261 0.002615

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -0.96 -2.54 -0.13 -2.39 -1.36 -2.73 -2.58 -1.19 -2.09 -1.51 -2.76 -3.50 -1.21 -3.05
Bud Neck -3.21 12.08 8.87 4.47 -2.97 -9.94 6.92 0.89 10.90 -10.56 -6.89 13.66 8.73 11.25 -6.39
Bud Site -3.74 0.77 -3.93 -0.02 -4.15 -4.69 -1.09 -7.82 -4.89 -7.10 -3.96 0.03 -8.34 -4.83 -8.01
Cell Periphery -1.57 -1.23 -1.81 0.02 -1.17 -1.89 1.59 1.98 4.00 0.08 -2.53 -0.23 -0.83 1.68 -0.79
Cytoplasm 10.91 12.52 9.39 -5.58 -2.54 14.83 27.26 17.77 3.06 -8.89 13.82 26.90 18.63 2.21 -7.56
Cytoplasmic Foci -4.92 47.48 46.79 18.03 -1.21 -15.36 36.57 35.59 44.97 -2.42 -11.13 58.67 57.82 48.08 -2.04
Eisosomes -1.44 13.62 12.29 5.94 -3.23 -4.66 5.99 5.96 14.77 -0.39 -4.42 13.20 12.47 15.61 -2.60
Endoplasmic Reticulum 0.86 -5.88 -14.28 -14.29 -9.61 2.24 -8.09 -10.09 -17.17 -2.71 3.07 -9.88 -17.39 -23.38 -9.18
Endosome -3.57 10.11 10.51 5.13 -0.79 -12.66 5.69 -0.09 11.86 -3.40 -10.42 11.31 7.88 13.78 -3.01
Golgi -1.11 8.13 2.44 2.18 -1.85 -4.57 3.00 -0.84 3.17 -2.64 -3.15 8.41 1.56 3.85 -3.16
Lipid Particles 2.38 13.05 13.61 6.22 0.40 -2.76 8.73 9.14 11.88 1.98 -1.56 15.44 16.14 12.44 1.56
Mitochondria -0.16 10.54 8.08 3.50 -1.27 -3.67 3.99 0.49 4.02 -3.07 -2.93 9.64 4.31 5.86 -3.28
Mitotic Spindle -0.80 -1.75 -3.83 -0.97 -2.24 -1.83 0.53 -7.64 -6.35 -7.99 -1.98 -1.39 -8.50 -6.11 -7.43
None -0.58 -5.27 -3.06 -0.20 3.48 0.14 -1.83 -2.97 -2.81 -1.49 -0.80 -5.49 -4.46 -2.21 2.21
Nuclear Periphery 2.16 -15.01 -17.08 -17.32 -7.09 -0.21 -18.97 -17.14 -16.48 -6.94 -0.49 -23.85 -23.39 -20.89 -10.09
Nuclear Periphery Foci -1.57 -5.43 -4.65 -3.66 -4.10 -1.78 -8.20 -7.62 -7.18 -3.10 -2.34 -9.80 -7.19 -6.77 -5.19
Nucleolus -2.04 -0.01 -0.75 0.34 -0.75 -3.42 0.72 0.33 4.58 -0.63 -4.35 0.67 -0.45 3.27 -0.89
Nucleus 2.16 -45.05 -27.95 -15.76 13.17 13.97 -39.34 -15.70 -26.82 21.31 10.85 -59.37 -30.97 -36.29 24.29
Peroxisomes -4.87 14.48 13.50 6.20 -2.23 -9.97 3.26 3.30 12.87 -0.44 -9.29 12.17 11.77 14.42 -1.21
Vacuole -3.31 0.54 -16.95 -6.42 -16.30 -13.52 -11.49 -24.39 -16.34 -12.98 -11.27 -8.57 -29.13 -18.86 -21.09
Vacuole Periphery -1.62 5.57 -0.85 -0.12 -1.77 -6.50 4.28 -1.52 2.90 -3.08 -6.09 6.95 -1.34 1.67 -2.85
ORF
Human Ortholog
Description Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
Localization
Cell Percentages cytoplasm (17%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJL107C

YJL107C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjl107c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available