Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 4 | 13 | 19 | 29 | 23 | 26 | 26 | 37 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 7 | 13 | 26 | 19 | 35 | 52 | 32 | 52 | 37 | 50 | 8 | 24 | 13 | 3 | 3 | 7 | 0 | 0 | 2 | 0 | 0 | 1 |
Cytoplasm | 307 | 249 | 140 | 180 | 297 | 285 | 378 | 423 | 253 | 255 | 241 | 224 | 358 | 464 | 536 | 174 | 225 | 248 | 289 | 242 | 146 | 392 | 388 | 423 |
Endoplasmic Reticulum | 7 | 3 | 9 | 9 | 10 | 17 | 55 | 61 | 55 | 66 | 45 | 70 | 9 | 17 | 6 | 7 | 5 | 9 | 9 | 6 | 3 | 16 | 28 | 27 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Golgi | 0 | 1 | 1 | 2 | 13 | 1 | 38 | 21 | 12 | 17 | 18 | 10 | 0 | 1 | 0 | 4 | 2 | 5 | 0 | 1 | 0 | 1 | 1 | 2 |
Mitochondria | 0 | 1 | 0 | 1 | 3 | 27 | 10 | 13 | 29 | 26 | 51 | 33 | 0 | 0 | 1 | 2 | 5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 5 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 38 | 14 | 23 | 47 | 25 | 13 | 33 | 17 | 0 | 1 | 0 | 7 | 17 | 12 | 3 | 2 | 1 | 1 | 4 | 2 |
Unique Cell Count | 316 | 255 | 150 | 192 | 349 | 341 | 503 | 580 | 366 | 385 | 395 | 366 | 364 | 479 | 546 | 185 | 243 | 266 | 310 | 260 | 156 | 419 | 433 | 474 |
Labelled Cell Count | 319 | 259 | 158 | 207 | 391 | 377 | 564 | 654 | 430 | 456 | 455 | 443 | 375 | 509 | 557 | 199 | 258 | 284 | 310 | 260 | 156 | 419 | 433 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.7 | 21.2 | 18.5 | 17.3 | 19.7 | 14.7 | 18.6 | 17.4 | 17.1 | 18.0 | 16.9 | 17.3 | 18.2 | 21.0 | 21.8 | 24.4 | 23.1 | 24.7 | 17.4 | 18.1 | 19.2 |
Std Deviation (1e-4) | 2.9 | 3.9 | 3.0 | 2.8 | 5.9 | 3.8 | 3.5 | 3.5 | 3.7 | 4.0 | 4.1 | 4.1 | 3.3 | 4.1 | 4.1 | 6.8 | 6.6 | 6.0 | 2.9 | 3.4 | 3.7 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.09 | -0.34 | 0.0 | -0.09 | -0.11 | -0.04 | -0.13 | -0.1 | -0.02 | 0.18 | 0.23 | 0.4 | 0.32 | 0.41 | -0.09 | -0.03 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.8 | 3.1 | 3.3 | 3.2 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.8 | 1.1 | 0.4 | 1.0 | 1.7 | 1.6 | 2.9 | 1.8 | 3.0 | 0 | 0.2 | 0 | 0 | 0 | -1.1 |
Cytoplasm | 0.2 | -2.6 | -2.9 | -4.8 | -5.3 | -5.9 | -6.4 | -7.3 | -7.3 | 3.0 | 1.9 | 3.1 | 0.3 | -0.3 | 0 |
Endoplasmic Reticulum | -0.5 | -1.7 | -0.5 | 1.8 | 1.7 | 2.8 | 3.3 | 1.9 | 3.8 | -2.0 | -1.3 | 0 | -0.9 | -2.0 | -1.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3.1 | 0 | 0 | 2.2 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 0 | 0 | 3.5 | 3.3 | 4.6 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.2 | 0 | 2.7 | 3.6 | 3.3 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.786 | 14.6225 | 14.0868 | 13.1055 | 11.7413 | 13.8 | 14.6223 | 20.7999 | 18.4995 | 18.4953 | 15.612 | 18.7037 | 17.7158 | 22.784 | 21.0821 | 19.6204 | 20.8354 | 21.0542 |
Actin | 0.0175 | 0.0009 | 0.0015 | 0.0015 | 0.0104 | 0.0014 | 0.0187 | 0.0004 | 0.0018 | 0.0006 | 0.0064 | 0.0018 | 0.0007 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.004 |
Bud | 0.0007 | 0.0003 | 0.001 | 0.0005 | 0.001 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0007 |
Bud Neck | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0008 | 0.001 | 0.0025 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.001 |
Bud Periphery | 0.0008 | 0.0001 | 0.0012 | 0.0002 | 0.001 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0012 |
Bud Site | 0.0013 | 0.0005 | 0.0007 | 0.0007 | 0.0008 | 0.0001 | 0.0018 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0011 |
Cell Periphery | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.7675 | 0.9622 | 0.9371 | 0.8335 | 0.7432 | 0.899 | 0.7841 | 0.983 | 0.9293 | 0.9135 | 0.8422 | 0.9221 | 0.8941 | 0.946 | 0.9542 | 0.9671 | 0.9747 | 0.9129 |
Cytoplasmic Foci | 0.0218 | 0.0048 | 0.0085 | 0.0425 | 0.0254 | 0.0132 | 0.0177 | 0.0029 | 0.0091 | 0.015 | 0.021 | 0.0078 | 0.0105 | 0.0295 | 0.0111 | 0.0048 | 0.0047 | 0.0087 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0542 | 0.0164 | 0.0118 | 0.0203 | 0.0173 | 0.0309 | 0.0532 | 0.0052 | 0.0147 | 0.0075 | 0.0133 | 0.0349 | 0.0551 | 0.0055 | 0.0163 | 0.0044 | 0.0081 | 0.0371 |
Endosome | 0.0493 | 0.007 | 0.02 | 0.0485 | 0.0931 | 0.0318 | 0.0453 | 0.0031 | 0.02 | 0.0178 | 0.0497 | 0.0198 | 0.0182 | 0.0056 | 0.0075 | 0.0111 | 0.0063 | 0.0169 |
Golgi | 0.0131 | 0.002 | 0.0018 | 0.0041 | 0.0237 | 0.0041 | 0.0073 | 0.0004 | 0.0032 | 0.001 | 0.0084 | 0.0037 | 0.002 | 0.003 | 0.001 | 0.0004 | 0.0003 | 0.0037 |
Lipid Particles | 0.0088 | 0.0001 | 0.0003 | 0.0125 | 0.0038 | 0.002 | 0.0081 | 0.0001 | 0.0027 | 0.0017 | 0.0121 | 0.0007 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Mitochondria | 0.0125 | 0.001 | 0.0056 | 0.0021 | 0.059 | 0.0033 | 0.0095 | 0.0001 | 0.0015 | 0.0007 | 0.0147 | 0.0015 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.003 |
None | 0.0035 | 0.0004 | 0.0006 | 0.0015 | 0.0014 | 0.0005 | 0.0033 | 0.0003 | 0.0009 | 0.0072 | 0.0028 | 0.0006 | 0.0021 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0011 |
Nuclear Periphery | 0.0166 | 0.0007 | 0.0009 | 0.0044 | 0.0034 | 0.0017 | 0.0142 | 0.0006 | 0.0041 | 0.0038 | 0.0132 | 0.0014 | 0.005 | 0.0006 | 0.0015 | 0.0019 | 0.0009 | 0.0026 |
Nucleolus | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0003 |
Nucleus | 0.0055 | 0.0006 | 0.0011 | 0.0014 | 0.002 | 0.0008 | 0.0082 | 0.0007 | 0.0032 | 0.0016 | 0.0022 | 0.0009 | 0.0018 | 0.0008 | 0.001 | 0.0012 | 0.0017 | 0.0014 |
Peroxisomes | 0.0033 | 0 | 0.0004 | 0.0019 | 0.0015 | 0.0047 | 0.0037 | 0 | 0.0004 | 0.0004 | 0.0011 | 0.0003 | 0.0002 | 0.0005 | 0.0012 | 0 | 0 | 0.0002 |
Punctate Nuclear | 0.0059 | 0.0001 | 0.0001 | 0.0011 | 0.0007 | 0.0011 | 0.0052 | 0 | 0.0004 | 0.0004 | 0.0025 | 0.0005 | 0.0008 | 0.0027 | 0.0001 | 0.0001 | 0.0001 | 0.0008 |
Vacuole | 0.0124 | 0.0018 | 0.0055 | 0.0173 | 0.007 | 0.0027 | 0.0116 | 0.0024 | 0.0056 | 0.0241 | 0.0061 | 0.0022 | 0.0057 | 0.0031 | 0.0034 | 0.0062 | 0.0016 | 0.0018 |
Vacuole Periphery | 0.0037 | 0.0003 | 0.0012 | 0.0051 | 0.0042 | 0.0012 | 0.0028 | 0.0002 | 0.0013 | 0.0036 | 0.0021 | 0.0008 | 0.001 | 0.0002 | 0.0006 | 0.0012 | 0.0005 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.1692 | 63.2886 | 26.345 | 43.0499 | 55.9707 | 32.7904 | 51.1148 | 52.6703 | 51.9627 | 44.1575 |
Translational Efficiency | 3.5211 | 2.7198 | 4.0475 | 2.5238 | 2.6407 | 4.5545 | 2.2738 | 2.221 | 2.1468 | 2.4772 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
292 | 855 | 177 | 1271 | 2122 | 2251 | 1048 | 1549 | 2414 | 3106 | 1225 | 2820 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1357.40 | 1378.17 | 1950.68 | 2021.05 | 1319.79 | 1399.84 | 1905.47 | 2219.84 | 1324.34 | 1393.87 | 1912.00 | 2130.24 |
Standard Deviation | 273.85 | 253.95 | 263.71 | 330.29 | 198.51 | 216.24 | 283.80 | 369.25 | 209.43 | 227.45 | 281.44 | 365.85 |
Intensity Change Log 2 | — | 0.021908 | 0.523131 | 0.574259 | — | 0.084954 | 0.529839 | 0.750147 | — | 0.053332 | 0.526442 | 0.663648 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000766 | 0.000433 | 0.001817 | 0.000698 | 0.000260 | 0.000366 | 0.000603 | 0.000782 | 0.000322 | 0.000385 | 0.000778 | 0.000744 |
Bud Neck | 0.000624 | 0.000763 | 0.008692 | 0.001947 | 0.000448 | 0.000673 | 0.001618 | 0.001391 | 0.000469 | 0.000698 | 0.002640 | 0.001642 |
Bud Site | 0.070761 | 0.025369 | 0.048419 | 0.022092 | 0.015645 | 0.017940 | 0.014741 | 0.017588 | 0.022312 | 0.019985 | 0.019607 | 0.019618 |
Cell Periphery | 0.014891 | 0.000926 | 0.007335 | 0.018400 | 0.000395 | 0.000262 | 0.004223 | 0.011343 | 0.002148 | 0.000445 | 0.004672 | 0.014524 |
Cytoplasm | 0.551843* | 0.834407* | 0.588300* | 0.774263* | 0.794347* | 0.822864* | 0.873969* | 0.843260* | 0.765014* | 0.826042* | 0.832693* | 0.812162* |
Cytoplasmic Foci | 0.184047* | 0.072985 | 0.067471 | 0.011303 | 0.115064 | 0.075471 | 0.012200 | 0.010091 | 0.123408 | 0.074787 | 0.020186 | 0.010637 |
Eisosomes | 0.000011 | 0.000005 | 0.000013 | 0.000008 | 0.000005 | 0.000005 | 0.000004 | 0.000006 | 0.000006 | 0.000005 | 0.000005 | 0.000007 |
Endoplasmic Reticulum | 0.022573 | 0.004798 | 0.002660 | 0.001640 | 0.010246 | 0.003265 | 0.001536 | 0.000832 | 0.011737 | 0.003687 | 0.001699 | 0.001196 |
Endosome | 0.010130 | 0.006221 | 0.020653 | 0.003153 | 0.005763 | 0.004899 | 0.001972 | 0.001616 | 0.006292 | 0.005263 | 0.004671 | 0.002309 |
Golgi | 0.056142 | 0.016641 | 0.025843 | 0.013562 | 0.016897 | 0.010803 | 0.011448 | 0.010853 | 0.021644 | 0.012410 | 0.013528 | 0.012074 |
Lipid Particles | 0.013647 | 0.004493 | 0.028482 | 0.038281 | 0.002029 | 0.001112 | 0.019093 | 0.027355 | 0.003435 | 0.002042 | 0.020450 | 0.032279 |
Mitochondria | 0.000396 | 0.000225 | 0.000028 | 0.000056 | 0.000352 | 0.000755 | 0.000020 | 0.000099 | 0.000358 | 0.000609 | 0.000021 | 0.000079 |
Mitotic Spindle | 0.002330 | 0.001826 | 0.009844 | 0.001584 | 0.000959 | 0.001933 | 0.000704 | 0.000979 | 0.001125 | 0.001904 | 0.002025 | 0.001252 |
None | 0.000558 | 0.000635 | 0.000152 | 0.000988 | 0.000312 | 0.000583 | 0.000656 | 0.000518 | 0.000342 | 0.000598 | 0.000583 | 0.000730 |
Nuclear Periphery | 0.000059 | 0.000034 | 0.000225 | 0.000146 | 0.000044 | 0.000028 | 0.000048 | 0.000056 | 0.000045 | 0.000030 | 0.000074 | 0.000097 |
Nuclear Periphery Foci | 0.003184 | 0.002846 | 0.013681 | 0.005131 | 0.001230 | 0.000396 | 0.006418 | 0.003696 | 0.001466 | 0.001070 | 0.007467 | 0.004343 |
Nucleolus | 0.001379 | 0.000065 | 0.000050 | 0.000075 | 0.000041 | 0.000064 | 0.000032 | 0.000072 | 0.000203 | 0.000065 | 0.000035 | 0.000074 |
Nucleus | 0.006026 | 0.001983 | 0.006091 | 0.003380 | 0.002032 | 0.003349 | 0.005536 | 0.004252 | 0.002515 | 0.002973 | 0.005616 | 0.003859 |
Peroxisomes | 0.000681 | 0.000437 | 0.000410 | 0.000290 | 0.000316 | 0.000495 | 0.000091 | 0.000323 | 0.000360 | 0.000479 | 0.000137 | 0.000308 |
Vacuole | 0.015506 | 0.017681 | 0.162984 | 0.096737 | 0.018923 | 0.044421 | 0.042796 | 0.060660 | 0.018510 | 0.037060 | 0.060162 | 0.076921 |
Vacuole Periphery | 0.044447 | 0.007229 | 0.006850 | 0.006266 | 0.014692 | 0.010315 | 0.002293 | 0.004227 | 0.018291 | 0.009465 | 0.002951 | 0.005146 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.98 | -4.14 | 0.26 | -3.94 | 4.44 | -1.08 | -3.24 | -3.99 | -2.85 | -0.70 | -0.82 | -4.49 | -5.28 | -3.97 | 0.61 |
Bud Neck | -1.24 | -9.12 | -9.20 | -8.34 | 7.41 | -2.21 | -7.87 | -8.96 | -6.37 | 1.28 | -2.76 | -11.23 | -13.16 | -10.26 | 4.61 |
Bud Site | 7.41 | 2.65 | 7.83 | 0.96 | 4.82 | -1.75 | 0.06 | -1.58 | -0.13 | -1.26 | 1.65 | 0.92 | 1.27 | -0.29 | 0.06 |
Cell Periphery | 4.96 | 2.44 | -0.82 | -11.24 | -4.72 | 3.71 | -9.78 | -11.23 | -11.38 | -6.67 | 4.79 | -4.46 | -12.87 | -15.97 | -9.95 |
Cytoplasm | -14.13 | -0.82 | -10.72 | 6.63 | -8.33 | -3.99 | -9.50 | -5.72 | -2.11 | 3.79 | -9.07 | -7.23 | -5.74 | 3.37 | 2.63 |
Cytoplasmic Foci | 9.05 | 9.10 | 14.86 | 14.16 | 10.08 | 9.53 | 29.19 | 30.29 | 24.57 | 1.74 | 12.42 | 29.03 | 33.47 | 28.28 | 6.39 |
Eisosomes | 4.90 | -1.14 | 2.37 | -4.70 | 3.66 | -0.48 | 0.96 | -2.10 | -1.50 | -2.69 | 1.42 | -0.14 | -2.83 | -4.01 | -2.43 |
Endoplasmic Reticulum | 5.13 | 5.95 | 6.26 | 3.14 | 1.98 | 5.64 | 6.65 | 8.14 | 4.97 | 1.07 | 6.88 | 8.25 | 9.57 | 5.40 | 0.88 |
Endosome | 3.41 | -4.92 | 6.51 | 3.89 | 8.69 | 1.78 | 10.46 | 11.11 | 7.65 | 1.29 | 2.33 | 3.25 | 10.21 | 7.24 | 5.29 |
Golgi | 7.65 | 5.70 | 8.40 | 2.08 | 4.16 | 5.98 | 4.71 | 5.61 | 0.08 | 0.54 | 8.33 | 6.63 | 8.72 | 0.64 | 1.30 |
Lipid Particles | 2.48 | -3.08 | -5.72 | -14.70 | -2.31 | 4.25 | -11.07 | -13.68 | -14.23 | -3.28 | 2.73 | -11.59 | -19.14 | -21.01 | -5.61 |
Mitochondria | 1.33 | 4.67 | 4.33 | 1.62 | -3.28 | -2.38 | 11.67 | 7.69 | 3.90 | -4.70 | -1.98 | 12.56 | 9.79 | 4.23 | -5.91 |
Mitotic Spindle | 0.67 | -4.58 | 0.63 | 0.02 | 4.88 | -1.51 | 1.26 | -0.08 | 1.45 | -1.18 | -1.56 | -2.98 | -0.66 | 1.00 | 1.87 |
None | -0.27 | 1.92 | -0.73 | -0.65 | -1.70 | -2.50 | -3.54 | -2.08 | 0.41 | 0.90 | -2.88 | -2.71 | -1.68 | -0.50 | -0.61 |
Nuclear Periphery | 1.62 | -5.65 | -1.32 | -1.68 | 1.53 | 1.37 | -0.66 | -1.36 | -5.22 | -1.46 | 1.52 | -3.21 | -1.70 | -2.28 | -0.57 |
Nuclear Periphery Foci | 0.40 | -6.41 | -2.10 | -4.74 | 5.95 | 2.74 | -10.40 | -6.70 | -14.08 | 5.87 | 1.32 | -12.19 | -8.43 | -14.54 | 6.91 |
Nucleolus | 1.04 | 1.05 | 1.03 | -0.50 | -4.10 | -3.54 | 1.83 | -5.37 | -1.46 | -8.97 | 0.92 | 1.09 | 0.85 | -1.32 | -11.46 |
Nucleus | 1.12 | -0.21 | 0.59 | -7.44 | 3.98 | -5.86 | -10.38 | -7.66 | -3.97 | 1.90 | -0.96 | -6.30 | -3.83 | -5.76 | 4.03 |
Peroxisomes | 1.31 | 1.38 | 2.07 | 2.39 | 1.79 | -2.71 | 4.54 | -0.09 | 2.90 | -5.61 | -1.94 | 4.45 | 0.96 | 3.91 | -6.09 |
Vacuole | -0.93 | -9.08 | -18.19 | -18.29 | 3.87 | -9.59 | -8.53 | -14.64 | -5.07 | -4.56 | -8.99 | -11.72 | -23.13 | -14.07 | -4.04 |
Vacuole Periphery | 7.02 | 7.22 | 7.34 | 0.97 | 0.34 | 3.52 | 12.04 | 9.76 | 7.27 | -4.15 | 7.03 | 13.69 | 11.50 | 6.29 | -5.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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