Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.16 0.06 0.1 0 0 0 0 – 0 0 0 0.06 0 0 – – 0.21 0.17 0.22 0.24 0.24 0.2 0.16
Bud 0 0.05 0 0 0.06 0.05 0.07 0 – 0.05 0.09 0.06 0 0 0 – – 0.06 0 0.05 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0.13 0.11 0.06 0.08 0.08 0.09
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0.09 0.05 0.1 0.09 0 0 0 0 – 0 0 0 0.06 0.1 0.05 – – 0.09 0.11 0.14 0.21 0.15 0.14 0.15
Cytoplasm 0.79 0.74 0.77 0.76 0.82 0.81 0.73 0.76 – 0.68 0.57 0.52 0.9 0.93 0.94 – – 0.6 0.38 0.31 0.22 0.28 0.38 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.06 0.1 0.06 0.21 0.35 0.3 – 0.47 0.59 0.61 0 0 0 – – 0.07 0.1 0.08 0.12 0.14 0.1 0.1
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 35 9 18 5 5 2 2 – 0 0 2 38 25 19 – – 45 46 56 39 48 77 48
Bud 10 10 6 8 14 14 17 9 – 19 19 18 3 9 5 – – 13 9 13 4 9 12 11
Bud Neck 6 1 1 2 6 2 1 4 – 5 0 3 23 22 18 – – 2 34 27 9 15 30 28
Bud Site 3 7 4 5 3 7 3 1 – 4 0 2 6 0 2 – – 5 – – – – – –
Cell Periphery 21 11 15 17 9 9 6 7 – 1 3 6 39 57 39 – – 20 29 36 34 30 55 44
Cytoplasm 188 162 117 143 189 209 170 234 – 236 116 168 544 553 679 – – 128 101 78 35 55 145 104
Endoplasmic Reticulum 5 6 1 1 1 0 0 0 – 0 0 6 5 3 5 – – 15 4 3 4 2 3 5
Endosome 0 0 0 0 0 2 0 1 – 0 0 2 0 0 1 – – 1 4 3 2 1 2 4
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 – – 0 0 0 2 0 0 1
Mitochondria 9 17 9 18 13 54 81 94 – 164 121 197 0 0 0 – – 15 26 20 19 28 38 31
Nucleus 0 0 1 0 0 1 1 0 – 2 1 1 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 1 7 6 7 8 2 4 – 0 0 0 0 0 0 – – 5 2 1 2 1 2 2
SpindlePole 7 1 3 7 9 3 7 11 – 10 9 9 24 9 8 – – 27 1 1 0 1 3 4
Vac/Vac Membrane 1 0 0 0 0 2 1 0 – 0 0 1 0 0 0 – – 3 6 5 3 2 7 6
Unique Cell Count 239 220 151 187 230 258 234 309 348 205 324 604 596 719 215 269 255 164 199 385 302
Labelled Cell Count 278 251 173 225 257 316 291 368 441 269 415 682 678 777 279 269 255 164 199 385 302


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.1 4.0 4.0 3.9 3.1 3.2 3.0 – 2.4 2.7 2.6 5.2 5.1 5.5 4.0 4.3 5.0 5.2 5.4 5.6
Std Deviation (1e-4) 0.7 1.0 1.6 1.6 1.6 1.1 1.7 1.3 – 0.9 0.8 0.6 0.8 0.6 0.9 1.0 0.8 0.9 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.01 -0.03 -0.35 -0.32 -0.4 – -0.71 -0.57 -0.64 0.39 0.34 0.46 0.01 0.1 0.34 0.38 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9288 1.1737 1.1623 2.0256 1.3021 1.5074 0.8802 1.3994 1.5278 2.4246 2.0179 1.8752 0.8591 0.8944 0.8805 1.3951 0.903 1.0838
Actin 0.0597 0.0235 0.0451 0.0237 0.0442 0.037 0.0475 0.0158 0.055 0.0095 0.0086 0.0658 0.0052 0.0075 0.0267 0.0046 0.0097 0.0322
Bud 0.0056 0.0092 0.0069 0.0191 0.0157 0.0062 0.0027 0.0018 0.005 0.0006 0.0032 0.0017 0.0014 0.0023 0.003 0.0021 0.0013 0.0017
Bud Neck 0.0382 0.0097 0.0645 0.0973 0.0631 0.1635 0.0357 0.0093 0.0545 0.1164 0.1685 0.1829 0.028 0.0098 0.0575 0.1588 0.2288 0.1689
Bud Periphery 0.0272 0.0232 0.0589 0.0521 0.0397 0.0401 0.0171 0.0069 0.0343 0.0073 0.031 0.0192 0.0056 0.0138 0.0147 0.0666 0.011 0.0275
Bud Site 0.0538 0.248 0.1279 0.0592 0.02 0.0176 0.1096 0.324 0.1077 0.0047 0.0425 0.0145 0.0361 0.2701 0.1171 0.0257 0.019 0.0039
Cell Periphery 0.1109 0.0724 0.1075 0.0925 0.106 0.099 0.2311 0.1094 0.1633 0.2088 0.2968 0.199 0.3073 0.0623 0.1378 0.1439 0.0967 0.1683
Cytoplasm 0.1766 0.2036 0.1965 0.2626 0.1807 0.2298 0.3468 0.2316 0.2252 0.3082 0.2507 0.274 0.2196 0.2268 0.2881 0.214 0.1887 0.3589
Cytoplasmic Foci 0.0163 0.0084 0.015 0.0218 0.0379 0.0183 0.023 0.0032 0.0176 0.0094 0.0103 0.0047 0.0043 0.0038 0.0051 0.0049 0.005 0.0161
Eisosomes 0.0099 0.0093 0.0042 0.0021 0.0026 0.0021 0.0083 0.0028 0.0059 0.0037 0.0015 0.0032 0.0088 0.0027 0.005 0.0026 0.0005 0.001
Endoplasmic Reticulum 0.0104 0.0024 0.0023 0.0053 0.0065 0.0026 0.0016 0.0009 0.0028 0.0038 0.0036 0.001 0.0023 0.0025 0.0022 0.0022 0.0008 0.0013
Endosome 0.0209 0.0007 0.0074 0.016 0.0172 0.0029 0.0018 0.0002 0.0039 0.0119 0.0011 0.0003 0.0001 0.0005 0.0003 0.0003 0.0001 0.0003
Golgi 0.0068 0.0012 0.0044 0.0052 0.0069 0.0013 0.0026 0.0004 0.0064 0.0234 0.0003 0.0006 0.0004 0.0004 0.0008 0.0003 0.0003 0.0005
Lipid Particles 0.0135 0.0008 0.008 0.0155 0.0257 0.0019 0.0105 0.0003 0.0049 0.0149 0.0002 0.0005 0.001 0.0007 0.0007 0.0012 0.0004 0.0015
Mitochondria 0.006 0.0014 0.0065 0.0068 0.0028 0.0011 0.0009 0.0002 0.0028 0.029 0.0028 0.0005 0.0003 0.0002 0.0002 0.0002 0 0.0002
None 0.3948 0.3713 0.3235 0.2417 0.3859 0.3548 0.1495 0.2915 0.3017 0.2369 0.131 0.2289 0.3765 0.3931 0.3368 0.3689 0.4361 0.2151
Nuclear Periphery 0.014 0.0005 0.0042 0.0121 0.0039 0.0042 0.0003 0.0001 0.0004 0.0003 0.0222 0.0001 0.0002 0.0003 0.0002 0.0002 0 0.0001
Nucleolus 0.0018 0.001 0.0023 0.003 0.0023 0.0011 0.0008 0.0003 0.0005 0.0005 0.0003 0.0003 0.0003 0.0005 0.0003 0.0003 0.0001 0.0001
Nucleus 0.0102 0.0022 0.0029 0.0185 0.0088 0.0051 0.0005 0.0004 0.0007 0.0006 0.0216 0.0004 0.0004 0.0007 0.0006 0.0004 0.0001 0.0003
Peroxisomes 0.007 0.0053 0.0061 0.0144 0.0189 0.0023 0.0064 0.0002 0.0042 0.0055 0.0002 0.0006 0.0002 0.0001 0.0003 0.0003 0.0003 0.0004
Punctate Nuclear 0.0062 0.0038 0.0014 0.0192 0.0074 0.0042 0.0011 0.0004 0.0006 0.0002 0.0005 0.0001 0.0004 0.0003 0.0008 0.0003 0.0002 0.0001
Vacuole 0.0078 0.002 0.0038 0.0093 0.0033 0.0047 0.0023 0.0007 0.0024 0.0038 0.0022 0.0018 0.0014 0.0014 0.0017 0.0021 0.0006 0.0015
Vacuole Periphery 0.0025 0.0001 0.0007 0.0024 0.0006 0.0003 0.0001 0 0.0002 0.0006 0.001 0.0001 0 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5462 8.2641 9.1984 7.5935 6.4211 11.2193 12.5337 10.8764 8.6212 10.7425
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
54 984 1071 144 192 1999 1324 1336 246 2983 2395 1480

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 993.16 674.84 856.66 909.62 681.84 704.95 827.09 835.54 750.18 695.02 840.31 842.75
Standard Deviation 166.63 87.32 102.25 122.68 86.43 86.00 102.13 114.20 168.91 87.59 103.24 117.13
Intensity Change Log 2 — -0.557481 -0.213303 -0.126762 — 0.048088 0.278611 0.293276 — -0.279712 0.007517 0.059198

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.018495 0.008667 0.007408 0.007220 0.002803 0.005837 0.025804 0.011510 0.006248 0.006770 0.017578 0.011092
Bud Neck 0.055332 0.049693 0.011241 0.010144 0.077930 0.079804 0.060206 0.029299 0.072970 0.069871 0.038310 0.027435
Bud Site 0.104941* 0.026876 0.039689 0.072639 0.021368 0.032422 0.085714 0.076149 0.039713 0.030593 0.065133 0.075807
Cell Periphery 0.075439 0.002069 0.001912 0.015553 0.022414 0.002977 0.022340 0.014523 0.034054 0.002677 0.013205 0.014623
Cytoplasm 0.179747* 0.414666* 0.444401* 0.270105* 0.423587* 0.397462* 0.382761* 0.434970* 0.370061* 0.403137* 0.410326* 0.418929*
Cytoplasmic Foci 0.044757 0.260948* 0.041415 0.059171 0.244888* 0.248504* 0.034870 0.034496 0.200957* 0.252609* 0.037796 0.036897
Eisosomes 0.008792 0.002962 0.000598 0.008445 0.015698 0.003971 0.008566 0.005335 0.014182 0.003638 0.005003 0.005637
Endoplasmic Reticulum 0.014883 0.000776 0.006848 0.008999 0.004057 0.000847 0.008341 0.002808 0.006433 0.000823 0.007674 0.003410
Endosome 0.048023 0.020721 0.003914 0.009235 0.010210 0.019362 0.001610 0.003123 0.018510 0.019810 0.002641 0.003718
Golgi 0.006389 0.005243 0.000263 0.004724 0.011646 0.007853 0.000497 0.000997 0.010492 0.006992 0.000393 0.001360
Lipid Particles 0.007060 0.010023 0.003180 0.005405 0.024612 0.011715 0.003269 0.002040 0.020759 0.011157 0.003229 0.002367
Mitochondria 0.004744 0.007424 0.000604 0.000271 0.008489 0.011871 0.000356 0.002641 0.007667 0.010404 0.000467 0.002410
Mitotic Spindle 0.000321 0.000727 0.001993 0.002376 0.000054 0.001577 0.000191 0.011925 0.000113 0.001297 0.000997 0.010996
None 0.000068 0.002431 0.002193 0.001077 0.000521 0.001023 0.001030 0.001374 0.000422 0.001488 0.001550 0.001346
Nuclear Periphery 0.003477 0.000259 0.001042 0.000586 0.000223 0.000285 0.000396 0.000623 0.000937 0.000276 0.000685 0.000619
Nuclear Periphery Foci 0.003741 0.000415 0.003117 0.003410 0.000231 0.000194 0.001081 0.001036 0.001002 0.000267 0.001991 0.001267
Nucleolus 0.000254 0.001146 0.000378 0.000151 0.000249 0.000650 0.000113 0.000231 0.000250 0.000813 0.000232 0.000224
Nucleus 0.063412 0.087087 0.345661* 0.133460* 0.075124 0.090158 0.276359* 0.203245* 0.072553 0.089145 0.307350* 0.196455*
Peroxisomes 0.005128 0.026643 0.001756 0.000893 0.022944 0.027211 0.002569 0.002009 0.019033 0.027023 0.002205 0.001900
Vacuole 0.345897* 0.069769 0.082177 0.385559* 0.030777 0.055151 0.083862 0.160752 0.099949 0.059973 0.083108 0.182625
Vacuole Periphery 0.009099 0.001456 0.000212 0.000578 0.002176 0.001127 0.000063 0.000915 0.003695 0.001236 0.000130 0.000882

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.86 1.05 1.25 2.10 1.23 -2.50 -8.92 -3.74 -1.91 5.86 -0.22 -3.49 -0.81 -1.16 4.20
Bud Neck 0.27 3.35 3.55 10.91 1.53 -0.15 1.93 4.83 14.07 7.71 0.30 3.96 5.43 14.97 4.81
Bud Site 3.09 2.73 1.91 -1.80 -1.12 -1.39 -6.90 -4.72 -5.91 3.00 1.12 -2.41 -2.32 -6.68 -0.06
Cell Periphery 2.91 2.93 2.62 -1.91 -2.05 2.88 0.32 1.70 -4.98 3.91 4.07 2.81 2.97 -5.47 0.80
Cytoplasm -8.58 -8.62 -2.55 6.93 6.98 1.47 2.73 0.94 -0.96 -3.21 -2.04 -1.35 -1.34 1.24 -0.06
Cytoplasmic Foci -18.59 0.30 -0.46 24.46 -1.34 -0.26 13.04 13.24 41.98 1.08 -3.70 11.83 12.09 51.64 1.70
Eisosomes 1.67 2.47 1.03 -0.88 -2.26 3.76 2.48 3.74 -0.03 3.75 4.12 3.74 3.92 -0.66 0.68
Endoplasmic Reticulum 2.33 1.13 1.24 -5.48 0.62 2.22 -2.55 0.71 -9.10 5.94 3.19 -0.99 1.68 -11.21 8.04
Endosome 1.99 3.29 3.07 5.22 -1.00 -5.01 5.70 4.79 14.19 -2.90 -0.56 4.84 4.70 17.07 -0.81
Golgi 0.32 2.11 0.99 1.12 -0.96 2.24 6.95 6.66 11.04 -0.93 2.38 7.19 6.58 10.96 -1.54
Lipid Particles -1.52 1.79 1.15 5.40 -2.03 3.58 5.96 6.39 13.71 2.15 3.33 6.10 6.52 15.97 3.14
Mitochondria -0.68 0.97 1.07 7.10 2.61 -1.74 4.92 3.56 7.92 -2.23 -1.58 4.58 3.39 8.47 -2.04
Mitotic Spindle -1.55 -2.15 -1.74 -1.64 -1.32 -2.78 -3.00 -5.36 -4.43 -5.29 -3.17 -2.58 -5.60 -4.85 -5.03
None -6.32 -12.95 -2.57 0.78 0.31 -2.83 -2.35 -3.44 -1.21 -1.29 -5.95 -7.07 -4.46 0.55 0.46
Nuclear Periphery 0.97 0.65 0.77 -7.00 3.61 -0.57 -4.73 -3.15 -2.45 -1.66 0.90 0.10 0.19 -3.03 0.41
Nuclear Periphery Foci 1.76 0.03 0.18 -5.98 0.54 0.52 -9.22 -8.25 -8.60 0.35 1.73 -2.93 -0.79 -10.05 6.16
Nucleolus -4.78 -1.02 -0.14 5.71 1.28 -6.58 3.25 -0.22 4.57 -1.84 -8.54 -0.09 -0.24 6.79 -0.20
Nucleus -0.53 -9.52 -2.43 -4.33 12.81 -2.01 -21.88 -15.05 -18.65 8.69 -1.72 -24.88 -13.15 -19.60 15.11
Peroxisomes -7.74 1.93 2.59 11.52 2.86 -1.07 5.51 5.69 15.21 1.42 -2.48 5.70 5.83 18.62 1.13
Vacuole 6.49 5.71 -1.36 -14.07 -12.64 -5.29 -11.80 -23.64 -23.23 -15.77 3.13 0.18 -8.93 -25.60 -18.79
Vacuole Periphery 1.17 1.37 1.35 3.83 -0.54 1.04 2.10 1.34 0.95 -1.42 1.51 2.22 1.82 1.39 -1.32
Standard name
Human Ortholog
Description Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Wsc3

Wsc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wsc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available