Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.93 0.99 0.98 0.99 0.95 0.86 0.76 0.74 0.65 0.68 0.59 0.58 0.98 0.98 0.99 0.97 – 0.97 0.66 0.83 0.7 0.68 0.69 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0.08 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0.11 0.3 0.41 0.42 0.52 0.43 0.63 0.61 0 0 0 0 – 0 0.16 0 0.13 0.14 0.12 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 – 1 2 9 7 4 3 6
Bud 1 0 0 0 3 8 11 10 11 13 10 4 0 0 0 0 – 0 2 10 1 5 4 7
Bud Neck 1 2 1 2 4 4 7 6 4 2 2 3 1 1 2 1 – 0 1 3 2 2 1 2
Bud Site 0 0 0 0 0 0 2 1 5 1 1 3 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 3 0 5 0 1 3 0 0 2 0 1 0 4 1 5 – 3 1 3 2 1 2 1
Cytoplasm 126 362 273 269 307 298 276 349 232 307 165 208 237 222 335 117 – 110 88 317 197 148 204 161
Endoplasmic Reticulum 2 3 1 1 0 0 1 1 0 0 0 0 2 3 2 7 – 9 2 6 14 3 5 3
Endosome 0 0 0 0 1 0 2 0 0 3 0 1 2 0 1 0 – 0 3 3 2 3 2 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 1 2 0 1 1
Mitochondria 11 1 11 10 35 104 148 198 185 196 178 217 0 0 0 5 – 2 21 14 37 30 35 32
Nucleus 0 0 0 0 1 1 0 0 2 2 2 4 0 0 2 0 – 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 3
Nucleolus 3 1 0 0 0 0 0 2 1 4 0 2 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 2 2 11 15 8 26 5 9 0 0 0 0 – 0 2 0 3 3 3 6
Vac/Vac Membrane 1 0 0 0 4 1 3 2 2 7 1 0 1 0 1 0 – 0 0 5 2 10 22 19
Unique Cell Count 135 366 279 272 323 347 362 470 355 454 281 357 241 226 340 120 113 134 382 282 218 297 261
Labelled Cell Count 148 374 288 287 357 420 464 585 450 563 364 452 244 231 344 135 126 134 382 282 218 297 261


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.1 3.7 3.5 3.3 3.0 2.8 2.9 2.5 2.6 2.4 2.5 4.9 4.7 4.8 4.9 4.8 5.0 5.4 5.4 5.5
Std Deviation (1e-4) 2.5 1.0 1.1 0.8 1.4 1.0 1.6 1.3 0.5 1.0 0.4 0.8 1.0 0.8 1.1 0.9 1.4 1.3 1.5 1.4 1.7
Intensity Change (Log2) – – – -0.09 -0.19 -0.29 -0.43 -0.37 -0.57 -0.51 -0.61 -0.56 0.41 0.33 0.38 0.4 0.38 0.42 0.55 0.55 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.573 -0.4426 -0.7026 -0.9552 -0.8953 -0.5851 1.0516 1.5561 1.501 2.0607 1.2828 1.6492 -0.6397 0.0073 -0.1884 0.1459 -0.3592 0.1288
Actin 0.019 0.0001 0.0024 0.0001 0.0007 0.0026 0.0072 0.0001 0.0084 0 0.04 0.0003 0 0.0003 0.0003 0.0002 0 0.0004
Bud 0.0003 0.0005 0.0008 0 0 0.0009 0.0004 0.0007 0.0004 0 0.0005 0.001 0 0.0008 0.0001 0 0 0.0003
Bud Neck 0.0096 0.0001 0.0003 0 0 0.0003 0.0005 0.0001 0.001 0 0.0004 0.0002 0 0.0003 0.0001 0 0 0.0075
Bud Periphery 0.001 0.0008 0.0027 0 0 0.0011 0.0017 0.0005 0.0008 0 0.0008 0.007 0 0.0004 0.0004 0 0 0.0002
Bud Site 0.0017 0.0039 0.0014 0 0.0001 0.0003 0.0075 0.0065 0.005 0 0.0011 0 0.0001 0.0031 0.0006 0 0 0.0004
Cell Periphery 0.0003 0.0001 0.0001 0 0 0.0001 0.0002 0.0003 0.0001 0 0.0001 0.0004 0 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0127 0.0128 0.0222 0.0295 0.0301 0.0198 0.0184 0.0229 0.0298 0.0034 0.0275 0.0778 0.0202 0.0402 0.0315 0.0332 0.0192 0.0681
Cytoplasmic Foci 0.0172 0.0049 0.0074 0.0039 0.0014 0.0087 0.0095 0.0035 0.0038 0.0009 0.0145 0.0072 0.0033 0.0025 0.0068 0.0015 0.001 0.003
Eisosomes 0.0006 0.0002 0.0003 0.0001 0.0002 0.0001 0.0004 0.0002 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0021 0.0006 0.0003 0.0007 0.0001 0.0003 0.0014 0.0005 0.0006 0.0001 0.0012 0.0015 0.0004 0.001 0.0006 0.0004 0.0001 0.0008
Endosome 0.007 0.0002 0.0004 0.0087 0 0.001 0.0124 0.0003 0.0006 0 0.009 0.0026 0.0008 0.0012 0.0007 0.0002 0 0.0011
Golgi 0.0023 0.0001 0.0003 0.0001 0 0.0004 0.011 0.0001 0.0007 0 0.0038 0.0003 0.0001 0.0001 0.0001 0 0 0
Lipid Particles 0.0095 0.0006 0.0005 0.0001 0 0.0009 0.0014 0.0003 0.0024 0.0001 0.0092 0.007 0.0008 0.0004 0.0001 0.0001 0 0.0001
Mitochondria 0.0014 0.0004 0.0015 0.0003 0.0001 0.0003 0.0028 0.0002 0.0005 0.0001 0.0019 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0001
None 0.8822 0.9718 0.9501 0.9539 0.9661 0.9584 0.9168 0.9597 0.9387 0.9949 0.8699 0.8909 0.9733 0.9402 0.9552 0.9633 0.9791 0.9154
Nuclear Periphery 0.0064 0.0004 0.0004 0.0005 0.0001 0.0015 0.0015 0.0004 0.0003 0.0001 0.0017 0.0005 0.0002 0.0011 0.0005 0.0001 0.0001 0.0002
Nucleolus 0.0011 0.0004 0.0014 0.0001 0.0001 0.0002 0.0005 0.0004 0.0002 0 0.0005 0.0001 0.0001 0.0006 0.0002 0 0 0.0001
Nucleus 0.0086 0.0006 0.0009 0.0003 0.0001 0.0004 0.0008 0.0012 0.0003 0 0.0005 0.0008 0.0001 0.0039 0.0009 0.0002 0.0001 0.0014
Peroxisomes 0.0109 0.0001 0.0027 0 0.0001 0.0004 0.001 0.0001 0.0047 0 0.0115 0.0002 0.0001 0.0001 0.0001 0 0 0
Punctate Nuclear 0.0046 0.001 0.0036 0.0003 0.0005 0.002 0.0024 0.001 0.001 0.0001 0.0046 0.0007 0.0003 0.0028 0.0012 0.0002 0.0002 0.0007
Vacuole 0.0007 0.0003 0.0002 0.0011 0.0001 0.0002 0.0019 0.001 0.0003 0.0001 0.0004 0.0008 0.0001 0.0005 0.0003 0.0001 0 0.0002
Vacuole Periphery 0.0006 0 0.0001 0.0003 0 0.0001 0.0004 0.0001 0 0 0.0003 0.0002 0 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
131 1631 367 414 2185 1812 1247 14 2316 3443 1614 428

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.16 714.28 717.84 841.91 700.03 659.83 796.48 383.25 696.30 685.62 778.60 826.91
Standard Deviation 62.98 94.06 98.58 130.25 83.14 83.35 135.36 5.34 83.53 92.67 132.11 151.88
Intensity Change Log 2 — 0.171643 0.178815 0.408819 — -0.085322 0.186221 -0.869131 — 0.042533 0.182706 -0.122994

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000506 0.000206 0.005026 0.000233 0.000885 0.000681 0.000493 0.000223 0.000705 0.000573 0.004877
Bud Neck 0.010694 0.046926 0.004493 0.015516 0.022893 0.058801 0.014729 0.000010 0.022203 0.053176 0.012401 0.015008
Bud Site 0.000900 0.018073 0.002362 0.057827 0.008744 0.025267 0.029273 0.000026 0.008300 0.021859 0.023154 0.055936
Cell Periphery 0.000267 0.000146 0.000227 0.001085 0.000228 0.000292 0.000616 0.000410 0.000230 0.000223 0.000527 0.001063
Cytoplasm 0.381633* 0.382354* 0.297362* 0.312805* 0.480903* 0.281909* 0.462093* 0.000099 0.475288* 0.329491* 0.424636* 0.302577*
Cytoplasmic Foci 0.325317* 0.217390* 0.033148 0.067737 0.217344* 0.284942* 0.031102 0.000029 0.223451* 0.252942* 0.031568 0.065523
Eisosomes 0.000341 0.000141 0.000070 0.000344 0.000229 0.000311 0.000066 0.002648 0.000236 0.000230 0.000067 0.000419
Endoplasmic Reticulum 0.004191 0.001299 0.017143 0.065239 0.009716 0.000910 0.006798 0.000002 0.009404 0.001094 0.009151 0.063105
Endosome 0.015158 0.025273 0.003944 0.016309 0.015600 0.045792 0.007866 0.000000 0.015575 0.036071 0.006974 0.015776
Golgi 0.001194 0.003827 0.000089 0.003856 0.003115 0.014889 0.001398 0.000009 0.003007 0.009648 0.001100 0.003730
Lipid Particles 0.036996 0.008696 0.003293 0.011066 0.014094 0.015751 0.002592 0.000136 0.015389 0.012409 0.002751 0.010708
Mitochondria 0.002566 0.012702 0.000527 0.010307 0.005310 0.050123 0.002412 0.000020 0.005155 0.032396 0.001984 0.009971
Mitotic Spindle 0.000024 0.007683 0.000645 0.022185 0.003499 0.014301 0.025671 0.000249 0.003303 0.011166 0.019980 0.021467
None 0.001391 0.003316 0.006543 0.004178 0.002862 0.002919 0.006184 0.994901* 0.002779 0.003107 0.006265 0.036584
Nuclear Periphery 0.002435 0.001120 0.002093 0.002570 0.001972 0.000610 0.003293 0.000000 0.001998 0.000852 0.003020 0.002486
Nuclear Periphery Foci 0.002044 0.000388 0.004185 0.013527 0.001178 0.000363 0.001853 0.000001 0.001227 0.000375 0.002383 0.013084
Nucleolus 0.004589 0.002112 0.001007 0.000413 0.001431 0.002535 0.001368 0.000001 0.001610 0.002335 0.001286 0.000399
Nucleus 0.143060* 0.143581* 0.563663* 0.103083 0.141923* 0.078973 0.311417* 0.000011 0.141987* 0.109579 0.368774* 0.099712
Peroxisomes 0.006938 0.014674 0.000415 0.005989 0.006786 0.035587 0.001670 0.000955 0.006794 0.025680 0.001385 0.005824
Vacuole 0.059523 0.105921 0.058390 0.279669* 0.060920 0.080441 0.086847 0.000000 0.060841 0.092511 0.080376 0.270521*
Vacuole Periphery 0.000687 0.003872 0.000196 0.001270 0.001018 0.004400 0.002072 0.000000 0.000999 0.004150 0.001645 0.001228

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.54 -4.77 -4.73 -3.92 -4.40 NaN NaN NaN NaN NaN -4.29 -2.07 -4.46 -3.62 -3.92
Bud Neck -7.39 1.37 -0.08 12.31 -2.90 NaN NaN NaN NaN NaN -13.69 5.97 6.00 18.28 0.86
Bud Site -11.56 -3.51 -6.27 -3.00 -5.96 NaN NaN NaN NaN NaN -9.12 -4.60 -4.77 -2.20 -2.73
Cell Periphery 3.71 1.17 -3.86 -5.36 -4.41 NaN NaN NaN NaN NaN 0.29 -2.98 -4.42 -4.53 -1.83
Cytoplasm -0.04 4.76 4.90 7.67 0.16 NaN NaN NaN NaN NaN 20.45 10.44 15.35 5.10 8.18
Cytoplasmic Foci 6.52 18.30 17.05 25.66 -3.25 NaN NaN NaN NaN NaN -4.94 42.56 30.27 37.93 -4.46
Eisosomes 5.58 7.19 2.02 -3.69 -5.65 NaN NaN NaN NaN NaN 0.32 13.80 -1.53 -1.61 -4.30
Endoplasmic Reticulum 1.43 -2.11 -4.45 -5.07 -2.49 NaN NaN NaN NaN NaN 6.57 0.71 -3.72 -5.09 -3.91
Endosome -3.47 4.68 1.87 6.26 -2.67 NaN NaN NaN NaN NaN -13.69 9.15 2.93 11.66 -1.91
Golgi -5.48 3.29 -0.76 2.49 -2.33 NaN NaN NaN NaN NaN -9.19 4.93 1.62 8.57 -1.38
Lipid Particles 3.49 4.16 3.56 0.62 -2.87 NaN NaN NaN NaN NaN 2.72 14.01 4.81 3.30 -3.46
Mitochondria -5.35 1.36 -0.77 2.75 -1.67 NaN NaN NaN NaN NaN -15.34 5.33 0.21 9.25 -1.21
Mitotic Spindle -5.17 -2.46 -3.71 -1.35 -3.54 NaN NaN NaN NaN NaN -5.33 -6.10 -2.61 -0.32 1.39
None -2.73 -3.42 -1.67 0.13 1.73 NaN NaN NaN NaN NaN -0.75 -5.35 -3.82 -3.78 -3.51
Nuclear Periphery 1.11 0.01 -0.29 -3.28 -0.72 NaN NaN NaN NaN NaN 3.23 -3.74 -1.22 -4.05 1.87
Nuclear Periphery Foci 2.67 -1.76 -4.10 -5.27 -2.76 NaN NaN NaN NaN NaN 4.32 -3.09 -4.74 -5.26 -4.09
Nucleolus 0.61 0.95 1.11 9.81 4.34 NaN NaN NaN NaN NaN -1.96 1.36 3.73 11.49 5.91
Nucleus -0.02 -20.95 1.42 2.30 24.63 NaN NaN NaN NaN NaN 7.99 -30.96 2.75 -1.05 25.11
Peroxisomes -2.92 2.63 1.53 10.37 -3.87 NaN NaN NaN NaN NaN -15.10 10.52 4.83 17.16 -2.67
Vacuole -6.30 -2.62 -20.91 -19.15 -18.69 NaN NaN NaN NaN NaN -10.73 -11.37 -21.69 -19.58 -17.71
Vacuole Periphery -5.04 3.54 0.15 4.87 -1.86 NaN NaN NaN NaN NaN -9.54 -0.34 1.57 8.95 1.48
Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (21%), ER (7%), mixed (28%)
Cell Cycle Regulation Yes
ER - T/G1 (0.00056)
Subcompartmental Group N/A

Izh4

Izh4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Izh4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available