Standard name
Human Ortholog
Description Putative metalloprotease; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0.06 0 0.06 – 0.05 0.05 0.05 0.05 0 0.07 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.98 0.97 0.96 0.91 0.84 0.82 – 0.8 0.84 0.98 0.99 0.99 0.97 0.98 0.98 0.96 0.98 0.93 0.88 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.14 – 0.16 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 4 3 – 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 1 1 2 1 3 0 – 0 2 1 0 3 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 14 0 12 14 12 34 26 17 – 13 22 15 23 19 45 41 34 0 0 0 0 0 0
Cytoplasm 105 344 111 232 334 333 521 589 221 – 196 363 291 475 548 601 632 618 100 345 102 86 271 500
Endoplasmic Reticulum 0 2 0 1 3 5 24 22 10 – 12 9 3 0 4 24 25 17 0 0 0 1 2 6
Endosome 0 0 0 0 1 0 0 1 0 – 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Mitochondria 1 1 1 3 1 10 21 51 39 – 38 72 1 0 3 3 3 2 0 0 0 0 0 0
Nucleus 0 0 2 3 4 2 1 3 0 – 1 2 2 4 4 14 5 2 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 – 0 1 1 0 0 1 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 0 0 – 0 1 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 1 4 5 44 3 – 2 11 0 2 1 7 7 9 0 1 3 6 8 9
Unique Cell Count 106 346 112 236 343 348 573 704 269 244 433 297 480 556 617 648 633 105 351 111 98 289 528
Labelled Cell Count 106 363 115 252 359 369 612 744 293 265 485 314 504 582 698 713 686 105 351 111 98 289 528


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.5 14.7 11.3 12.2 12.2 11.2 10.1 9.9 9.0 – 9.2 8.2 16.4 16.2 14.0 13.9 14.7 14.7 13.8 15.9 16.7
Std Deviation (1e-4) 3.1 1.9 2.2 1.7 1.9 1.9 1.8 2.0 1.8 – 1.9 1.8 3.0 2.6 2.4 3.0 2.9 3.2 2.8 3.1 3.4
Intensity Change (Log2) – – – 0.11 0.12 -0.01 -0.15 -0.19 -0.32 – -0.3 -0.46 0.54 0.52 0.31 0.3 0.39 0.39 0.29 0.5 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.6 0 0 – 0 0 0 0 0 3.0 2.7 2.5
Cytoplasm -0.6 -1.1 -1.7 -3.0 -4.3 -4.5 – -4.8 -4.3 -0.8 -0.1 -0.5 -1.1 -1.0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.6 3.9 – 4.1 4.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.7 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.8969 28.3311 35.0057 39.5978 34.9276 34.8841 25.6241 36.1215 32.0612 31.1169 29.3981 42.801 31.8066 29.7349 38.3308 35.0247 24.6007 35.8431
Actin 0.0027 0.0068 0.0123 0.0047 0.0104 0.0096 0.0454 0.0097 0.009 0.026 0.0037 0.0142 0.0179 0.0018 0.009 0.0041 0.0281 0.0037
Bud 0.0082 0.0249 0.0115 0.0003 0.0185 0.0029 0.0018 0.0173 0.0049 0.0306 0.0089 0.0005 0.0037 0.0099 0.0112 0.0002 0.0017 0.0016
Bud Neck 0.0004 0.0011 0.0006 0.0002 0.0016 0.0022 0.0095 0.0004 0.0006 0.0004 0.0004 0.0016 0.0002 0.0018 0.0004 0.0003 0.0012 0.0012
Bud Periphery 0.0126 0.0212 0.0103 0.0009 0.0323 0.0043 0.002 0.0073 0.0054 0.0463 0.0035 0.0006 0.0061 0.0046 0.0069 0.0004 0.0031 0.0009
Bud Site 0.005 0.0326 0.0139 0.0003 0.0035 0.001 0.0224 0.0184 0.0046 0.0023 0.0019 0.0004 0.0041 0.0226 0.0141 0.0011 0.0053 0.002
Cell Periphery 0.001 0.0031 0.001 0.0011 0.0012 0.0011 0.0019 0.0035 0.001 0.0008 0.0018 0.0001 0.0006 0.0055 0.0004 0.0013 0.0007 0.0003
Cytoplasm 0.0145 0.0051 0.0143 0.0056 0.0103 0.0063 0.3603 0.3141 0.3271 0.1505 0.2322 0.2672 0.0005 0.023 0.0093 0.0001 0.1281 0.0332
Cytoplasmic Foci 0.0914 0.0459 0.0292 0.0302 0.0325 0.0491 0.0643 0.0142 0.0321 0.0133 0.1264 0.0288 0.049 0.0568 0.017 0.0035 0.0158 0.0607
Eisosomes 0.0009 0.0016 0.001 0.0008 0.0004 0.0009 0.001 0.0018 0.0004 0.003 0.0002 0.0004 0.001 0.0007 0.0006 0.0008 0.0003 0.0002
Endoplasmic Reticulum 0.0002 0.0003 0.0002 0.0003 0.0014 0.0002 0.0032 0.0006 0.0015 0.0004 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0054 0.0002
Endosome 0.0007 0.0012 0.0013 0.0041 0.0008 0.0008 0.0288 0.0027 0.0123 0.0009 0.0128 0.0006 0.0009 0.0006 0.0014 0.0004 0.0644 0.0038
Golgi 0.0007 0.0006 0.0012 0.0029 0.0011 0.0015 0.0118 0.002 0.0045 0.0014 0.0027 0.0015 0.0024 0.001 0.0024 0.0007 0.0533 0.0051
Lipid Particles 0.0123 0.0074 0.011 0.0388 0.0021 0.008 0.0177 0.0073 0.0047 0.0013 0.0226 0.0039 0.0182 0.012 0.0145 0.0051 0.0132 0.0132
Mitochondria 0.5696 0.4323 0.5965 0.6699 0.5506 0.6077 0.3022 0.3961 0.3924 0.4195 0.4521 0.5602 0.6814 0.5331 0.7299 0.8029 0.5413 0.666
None 0.0072 0.0053 0.0056 0.0065 0.0065 0.0028 0.009 0.0022 0.0037 0.0058 0.0152 0.0014 0.0031 0.0058 0.0045 0.0003 0.0009 0.0023
Nuclear Periphery 0.0017 0.0043 0.0116 0.003 0.0043 0.0009 0.0037 0.0065 0.0031 0.0023 0.0002 0.0011 0.0002 0.0022 0.0019 0.047 0.0063 0.0003
Nucleolus 0.0405 0.0441 0.0233 0.0165 0.0272 0.0373 0.0178 0.0054 0.016 0.0071 0.0031 0.0204 0.0162 0.054 0.0201 0.0035 0.0036 0.0365
Nucleus 0.1706 0.2602 0.1677 0.1037 0.2312 0.1555 0.0636 0.1667 0.1352 0.2098 0.0035 0.0738 0.1699 0.1996 0.1062 0.1109 0.1118 0.1273
Peroxisomes 0.014 0.0093 0.0108 0.004 0.0027 0.0139 0.024 0.0021 0.0079 0.0062 0.0095 0.007 0.0063 0.0141 0.0072 0.0072 0.0032 0.0032
Punctate Nuclear 0.0367 0.0757 0.0547 0.0383 0.0537 0.0797 0.0044 0.0007 0.0246 0.0687 0.0925 0.0148 0.0031 0.0231 0.037 0.0052 0.0043 0.0241
Vacuole 0.0023 0.0086 0.0042 0.0081 0.0028 0.0036 0.0038 0.0071 0.0027 0.0022 0.0043 0.0004 0.0036 0.0123 0.0015 0.0017 0.0046 0.0016
Vacuole Periphery 0.0067 0.0085 0.0179 0.0597 0.0048 0.0107 0.0014 0.0138 0.0062 0.0013 0.0022 0.0008 0.0114 0.015 0.0043 0.0031 0.0034 0.0127

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.3121 53.5317 66.048 64.0182 49.8463 55.9542 60.291 56.0939 61.5512 58.8132
Translational Efficiency 0.9425 0.8181 0.7465 0.8106 0.7413 0.901 0.788 0.6661 0.6565 0.6331

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
921 910 165 686 709 1560 91 1688 1630 2470 256 2374

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2289.24 3433.52 3570.34 3280.25 2163.27 3226.09 2661.13 3125.42 2234.45 3302.51 3247.14 3170.16
Standard Deviation 728.29 1509.41 1769.75 840.51 1068.90 1119.99 620.40 879.65 894.75 1281.26 1531.30 871.35
Intensity Change Log 2 — 0.584820 0.641193 0.518937 — 0.576573 0.298825 0.530836 — 0.580819 0.484954 0.524731

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005739 0.009619 0.006722 0.007132 0.013723 0.008017 0.068184 0.005418 0.009212 0.008607 0.028570 0.005913
Bud Neck 0.002774 0.009013 0.012753 0.001396 0.009754 0.001702 0.005355 0.001040 0.005810 0.004396 0.010123 0.001143
Bud Site 0.006787 0.028686 0.013883 0.001143 0.021945 0.003398 0.001781 0.000971 0.013380 0.012715 0.009581 0.001021
Cell Periphery 0.000877 0.004973 0.004132 0.000321 0.003640 0.000329 0.001017 0.000627 0.002079 0.002040 0.003025 0.000539
Cytoplasm 0.001900 0.020239 0.001047 0.000286 0.002966 0.000637 0.000179 0.000144 0.002364 0.007859 0.000739 0.000185
Cytoplasmic Foci 0.029910 0.022490 0.109991 0.013620 0.029778 0.018626 0.020356 0.005547 0.029853 0.020050 0.078128 0.007880
Eisosomes 0.006299 0.009265 0.007219 0.006310 0.013814 0.006108 0.007406 0.006768 0.009567 0.007271 0.007285 0.006636
Endoplasmic Reticulum 0.000219 0.000497 0.000353 0.000070 0.000610 0.000166 0.002089 0.000082 0.000389 0.000288 0.000970 0.000078
Endosome 0.000416 0.002457 0.003991 0.000511 0.002621 0.000788 0.008198 0.000235 0.001375 0.001403 0.005487 0.000315
Golgi 0.004515 0.006764 0.014466 0.009414 0.006535 0.007748 0.178451* 0.004599 0.005393 0.007385 0.072757 0.005991
Lipid Particles 0.005629 0.003723 0.008992 0.001991 0.008018 0.007157 0.017538 0.000886 0.006668 0.005892 0.012030 0.001205
Mitochondria 0.866513* 0.823878* 0.707423* 0.928408* 0.805154* 0.913777* 0.616103* 0.956157* 0.839824* 0.880657* 0.674962* 0.948138*
Mitotic Spindle 0.000252 0.000346 0.005282 0.001174 0.001356 0.000215 0.000789 0.000150 0.000732 0.000263 0.003685 0.000446
None 0.009112 0.017383 0.007530 0.003111 0.011327 0.004993 0.004783 0.002721 0.010075 0.009558 0.006554 0.002834
Nuclear Periphery 0.000323 0.000146 0.001229 0.000273 0.000271 0.000194 0.001715 0.000199 0.000301 0.000176 0.001402 0.000220
Nuclear Periphery Foci 0.000306 0.000263 0.000825 0.000137 0.000602 0.000186 0.000546 0.000116 0.000435 0.000215 0.000726 0.000122
Nucleolus 0.005565 0.005996 0.013686 0.000848 0.007926 0.003151 0.000419 0.000956 0.006592 0.004199 0.008970 0.000925
Nucleus 0.003505 0.002293 0.001951 0.000060 0.001813 0.000602 0.000072 0.000171 0.002769 0.001225 0.001283 0.000139
Peroxisomes 0.016005 0.016431 0.030374 0.015748 0.015867 0.009968 0.003076 0.004617 0.015945 0.012349 0.020670 0.007833
Vacuole 0.015464 0.009293 0.026475 0.002782 0.022007 0.006606 0.019922 0.003012 0.018310 0.007596 0.024145 0.002945
Vacuole Periphery 0.017889 0.006245 0.021675 0.005266 0.020275 0.005632 0.042022 0.005585 0.018927 0.005858 0.028908 0.005493

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.12 -0.63 -0.95 1.41 -0.17 1.96 -2.96 3.00 1.72 3.45 0.37 -2.82 2.30 2.32 3.35
Bud Neck -3.59 -4.02 2.95 4.48 4.62 5.27 2.25 5.73 2.23 3.44 1.49 -2.41 6.51 4.94 5.42
Bud Site -5.45 -1.30 3.65 7.40 2.43 4.85 5.40 5.66 2.40 1.36 0.30 0.99 6.73 7.63 2.52
Cell Periphery -2.83 -1.09 2.48 3.25 1.29 4.05 2.95 3.67 -1.61 1.00 0.07 -0.48 4.02 2.76 1.30
Cytoplasm -4.91 1.05 2.89 5.43 1.64 2.71 3.65 3.69 1.49 0.69 -3.75 2.77 4.74 5.56 1.82
Cytoplasmic Foci 2.16 -5.06 4.98 3.04 6.13 3.49 1.68 8.47 7.24 2.97 4.25 -4.51 10.67 7.96 6.64
Eisosomes -2.96 -0.80 -0.01 2.33 0.65 5.58 1.61 4.96 -0.75 0.17 2.89 1.41 3.49 0.88 0.41
Endoplasmic Reticulum -2.70 -1.70 4.20 4.36 3.91 5.08 -2.29 6.28 2.55 3.14 1.78 -2.42 7.33 4.99 3.76
Endosome -3.54 -2.14 -0.59 3.27 2.07 3.37 -2.63 4.98 2.11 3.85 -0.08 -3.11 4.86 3.98 3.96
Golgi -1.99 -2.23 -2.72 -1.40 1.07 -0.86 -6.67 1.52 2.66 6.75 -2.21 -6.26 -0.64 1.42 6.20
Lipid Particles 1.85 -1.32 3.45 2.01 2.81 0.47 -2.00 4.98 5.47 3.66 0.71 -2.25 6.74 6.01 4.79
Mitochondria 3.32 5.59 -6.49 -8.70 -7.87 -8.92 4.67 -13.13 -7.31 -8.72 -4.98 7.02 -15.46 -11.76 -12.02
Mitotic Spindle -1.27 -2.60 -1.27 -1.14 1.98 1.12 0.55 1.19 0.76 3.20 1.05 -2.21 0.58 -0.85 2.55
None -2.57 1.41 7.67 4.56 5.71 6.05 5.55 8.42 7.24 3.27 0.39 4.24 11.55 5.72 6.75
Nuclear Periphery 4.39 -4.21 0.77 -2.09 4.34 1.46 -4.17 0.80 -0.07 4.32 3.90 -5.93 1.28 -0.77 6.16
Nuclear Periphery Foci 0.80 -3.56 2.23 1.65 4.37 3.12 0.37 3.72 1.26 3.87 3.34 -2.52 4.73 2.12 5.80
Nucleolus -0.31 -2.05 6.27 4.29 3.29 3.88 6.83 6.17 3.47 -2.04 2.83 -0.90 8.58 5.45 3.15
Nucleus 1.13 1.19 3.19 4.83 2.51 1.72 2.62 2.44 2.07 -1.47 2.22 1.77 3.87 4.98 2.32
Peroxisomes -0.14 -2.41 0.07 0.20 2.37 2.62 4.94 5.33 3.78 -0.81 1.98 -1.18 4.56 3.14 3.34
Vacuole 2.77 -1.73 6.62 4.70 3.87 5.78 0.35 7.43 3.23 3.12 6.37 -1.27 9.83 5.29 4.83
Vacuole Periphery 5.24 -0.72 5.85 0.70 3.28 4.88 -2.51 4.84 0.04 4.44 7.32 -2.15 7.43 0.41 5.35
Standard name
Human Ortholog
Description Putative metalloprotease; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
Localization
Cell Percentages mitochondrion (88%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Sdd3

Sdd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available