Standard name
Human Ortholog
Description O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06
Bud 0.08 0 0.05 0.05 0.07 0.06 0.06 0.09 0.07 0.06 0.06 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.21 0.71 0.63 0.6 0.39 0.26 0.17 0.18 0.11 0.12 0.08 0.1 0.82 0.8 0.81 0.07 0.12 0.13 0.07 0.29 0.2 0.13 0.11 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.22 0.25 0.17 0.09 0.11 0.13 0.13 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria 0.39 0.11 0.17 0.25 0.5 0.69 0.83 0.74 0.87 0.85 0.86 0.88 0 0 0 0.22 0.11 0.11 0.3 0.28 0.39 0.41 0.43 0.36
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.15 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.33 0.38 0.37 0.15 0 0 0 0.05 0.05
SpindlePole 0.29 0.12 0.17 0.15 0.17 0.14 0.14 0.16 0.11 0.14 0.12 0.07 0.08 0.12 0.11 0.48 0.39 0.39 0.13 0.15 0.14 0.11 0.1 0.12
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 9 14 6 2 1 1 6 27 9 13 23 33
Bud 7 19 13 8 13 15 23 27 24 24 23 30 4 7 5 7 6 5 1 14 3 3 11 18
Bud Neck 0 10 10 0 5 2 5 3 2 4 0 3 10 18 23 2 3 1 0 11 3 7 15 19
Bud Site 2 1 1 0 0 8 3 5 3 9 5 9 2 0 0 4 1 2 – – – – – –
Cell Periphery 3 6 1 4 0 0 0 0 0 1 1 3 1 4 3 2 3 9 0 4 2 2 4 6
Cytoplasm 18 379 179 94 77 69 61 56 40 48 31 44 306 370 459 10 18 22 8 192 66 57 65 63
Endoplasmic Reticulum 0 3 1 1 0 2 0 1 0 0 0 5 2 8 3 2 3 10 0 6 3 3 4 3
Endosome 11 26 7 6 1 2 0 2 3 5 1 2 15 8 24 21 34 43 20 55 36 58 73 72
Golgi 0 2 0 0 0 1 0 0 0 0 0 0 2 0 0 3 3 2 5 4 0 5 6 11
Mitochondria 34 58 49 40 100 179 295 233 306 335 321 388 7 1 2 32 16 19 36 180 125 181 249 214
Nucleus 0 0 0 0 1 4 5 8 4 5 8 2 0 0 2 3 1 0 0 1 0 0 0 1
Nuclear Periphery 0 3 1 0 0 0 1 1 2 1 9 4 0 2 0 1 0 0 0 2 0 2 0 1
Nucleolus 3 1 0 1 1 2 4 3 5 3 2 9 0 0 0 3 1 1 0 1 0 1 4 0
Peroxisomes 13 9 8 3 5 3 7 11 9 19 14 9 6 0 3 49 58 63 18 13 8 18 26 30
SpindlePole 25 64 48 24 34 36 51 50 37 57 44 32 31 56 61 71 60 66 15 96 43 49 54 70
Vac/Vac Membrane 3 11 3 1 0 2 3 1 6 6 5 2 7 4 8 5 5 9 2 21 5 19 22 29
Unique Cell Count 87 537 284 157 200 261 356 314 352 395 374 440 372 461 568 148 152 171 123 652 324 439 576 592
Labelled Cell Count 119 594 321 182 237 325 458 401 441 517 464 542 402 492 599 217 213 253 123 652 324 439 576 592


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 4.0 3.4 3.6 3.3 3.3 2.7 3.1 2.9 3.1 3.0 2.9 5.1 4.7 5.0 5.8 5.7 5.6 5.4 5.5 6.2
Std Deviation (1e-4) 3.7 0.9 1.0 1.7 1.6 1.1 0.9 1.2 0.8 1.0 0.9 0.8 1.0 0.7 1.0 1.3 1.4 1.3 1.3 1.3 11.3
Intensity Change (Log2) – – – 0.08 -0.04 -0.03 -0.33 -0.11 -0.22 -0.14 -0.18 -0.2 0.59 0.49 0.58 0.78 0.75 0.74 0.68 0.69 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3937 0.667 0.602 1.0361 0.2778 0.7178 3.7808 3.1038 3.4278 2.3774 3.4083 3.4659 1.5332 1.5124 1.5651 1.4543 1.3869 1.384
Actin 0.0107 0.0126 0.0051 0.0199 0.0027 0.0069 0.0965 0.0405 0.0335 0.0465 0.0419 0.0362 0.0124 0.0042 0.0101 0.0039 0.0064 0.004
Bud 0.0004 0.0016 0.0008 0.0021 0.0002 0.0006 0.001 0.0049 0.0006 0.0005 0.0009 0.0007 0.0004 0.001 0.0012 0.001 0.0009 0.0003
Bud Neck 0.001 0.0009 0.0004 0.002 0.0003 0.0011 0.0016 0.0006 0.0122 0.0002 0.0014 0.0024 0.001 0.0009 0.0008 0.0003 0.0006 0.0013
Bud Periphery 0.0008 0.0024 0.0023 0.0052 0.0005 0.0008 0.0015 0.0114 0.001 0.0006 0.0012 0.0015 0.0007 0.002 0.0028 0.004 0.0024 0.0006
Bud Site 0.0017 0.0172 0.003 0.0013 0.0005 0.0007 0.0053 0.0112 0.0072 0.0007 0.0016 0.0007 0.0054 0.0084 0.0022 0.0014 0.0022 0.0006
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0.0002 0.0009 0.0005 0.0006 0.0001 0.0001 0.0002 0.0004 0.0004 0.0003 0.0003 0.0008 0.0001
Cytoplasm 0.006 0.0043 0.0047 0.0053 0.0053 0.0085 0.0111 0.0048 0.0107 0.0014 0.0015 0.0024 0.0027 0.0057 0.0013 0.0049 0.0091 0.0015
Cytoplasmic Foci 0.1637 0.1573 0.151 0.1233 0.103 0.1336 0.1188 0.1396 0.1549 0.0809 0.1587 0.1305 0.1347 0.1669 0.1294 0.0988 0.1152 0.1233
Eisosomes 0.0004 0.0001 0.0003 0.0002 0.0003 0.0004 0.001 0.0008 0.0004 0.0002 0.0001 0.0008 0.0002 0.0001 0.0002 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0025 0.0005 0.0005 0.0006 0.0005 0.0011 0.0098 0.001 0.0005 0.0003 0.0001 0.001 0.0013 0.0004 0.0002 0.0002 0.0023 0.0002
Endosome 0.084 0.0705 0.0524 0.0856 0.0771 0.053 0.0746 0.0818 0.0928 0.039 0.0485 0.0527 0.093 0.0882 0.0744 0.0836 0.0836 0.0612
Golgi 0.0453 0.0586 0.0323 0.0497 0.0384 0.0366 0.0598 0.088 0.1013 0.0367 0.0492 0.0721 0.0725 0.0859 0.0797 0.0755 0.0596 0.0687
Lipid Particles 0.1445 0.0614 0.0839 0.028 0.0457 0.1034 0.0853 0.0386 0.0395 0.1227 0.0173 0.0717 0.142 0.0604 0.0671 0.0414 0.079 0.092
Mitochondria 0.1059 0.1193 0.1782 0.2697 0.1674 0.1437 0.0658 0.0868 0.1296 0.1497 0.0838 0.0829 0.1349 0.1615 0.2215 0.1107 0.1358 0.1649
None 0.0469 0.0249 0.0454 0.0335 0.0628 0.0491 0.0125 0.0099 0.0017 0.0007 0.0013 0.0283 0.0088 0.0126 0.0091 0.003 0.0273 0.009
Nuclear Periphery 0.0071 0.0005 0.0003 0.0004 0.001 0.0015 0.0035 0.0004 0.0003 0.0004 0.0001 0.0012 0.0027 0.0002 0.0003 0.0001 0.0053 0.0003
Nucleolus 0.0011 0.0008 0.0009 0.0002 0.0012 0.0011 0.0002 0.0003 0.0024 0.0003 0.0001 0.0002 0.001 0.0035 0.0004 0.0002 0.0005 0.0003
Nucleus 0.0014 0.0005 0.0004 0.0002 0.0015 0.0011 0.0007 0.0002 0.0005 0.0001 0.0001 0.0003 0.0022 0.0004 0.0002 0.0001 0.0009 0.0002
Peroxisomes 0.3658 0.4594 0.4203 0.3666 0.4854 0.4425 0.4432 0.4755 0.4009 0.5162 0.5895 0.51 0.3774 0.3907 0.3893 0.5662 0.4581 0.4645
Punctate Nuclear 0.0047 0.0026 0.0126 0.0007 0.0029 0.01 0.0041 0.0009 0.002 0.0011 0.0011 0.0024 0.0035 0.0023 0.0065 0.0021 0.0062 0.004
Vacuole 0.0035 0.0034 0.0034 0.0028 0.0021 0.0027 0.0018 0.0013 0.005 0.0009 0.0008 0.0011 0.0017 0.0031 0.0016 0.0012 0.002 0.0016
Vacuole Periphery 0.0024 0.0011 0.0016 0.0024 0.0011 0.0013 0.0011 0.0009 0.0022 0.0008 0.0006 0.0009 0.0012 0.0013 0.0013 0.001 0.0013 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2256 6.3027 8.5743 11.2433 11.4266 12.9188 11.6832 17.2045 17.0257 13.7441
Translational Efficiency 2.0133 1.9545 1.4458 1.6341 1.1437 1.3055 1.3868 1.0794 1.3169 1.3262

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1295 1516 2769 1260 1411 1353 2359 1472 2706 2869 5128 2732

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 623.37 713.45 842.32 776.10 650.80 677.68 788.44 808.75 637.67 696.58 817.53 793.69
Standard Deviation 73.94 100.55 109.34 115.06 88.59 91.85 102.69 113.59 83.05 98.18 109.67 115.42
Intensity Change Log 2 — 0.194724 0.434280 0.316154 — 0.058390 0.276787 0.313480 — 0.126699 0.355987 0.314789

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000439 0.002559 0.007739 0.015396 0.000944 0.001013 0.005198 0.008880 0.000703 0.001830 0.006570 0.011885
Bud Neck 0.046082 0.048011 0.036781 0.029667 0.047004 0.045830 0.052585 0.041194 0.046563 0.046983 0.044051 0.035878
Bud Site 0.010793 0.033874 0.045711 0.080900 0.015395 0.017431 0.057636 0.083154 0.013193 0.026120 0.051197 0.082114
Cell Periphery 0.000225 0.000198 0.000139 0.000201 0.000147 0.000308 0.000144 0.000260 0.000184 0.000250 0.000141 0.000233
Cytoplasm 0.141777* 0.086772 0.045330 0.133057* 0.099425 0.072408 0.082156 0.253245* 0.119693* 0.079998 0.062271 0.197815*
Cytoplasmic Foci 0.363923* 0.273371* 0.103777 0.133518 0.319687* 0.277053* 0.117776 0.121128 0.340857* 0.275108* 0.110216 0.126842
Eisosomes 0.000378 0.000235 0.000093 0.000113 0.000257 0.000318 0.000079 0.000160 0.000315 0.000274 0.000086 0.000138
Endoplasmic Reticulum 0.001288 0.000641 0.002948 0.001981 0.000772 0.000759 0.001175 0.002153 0.001019 0.000697 0.002132 0.002074
Endosome 0.079589 0.076279 0.089840 0.056504 0.097064 0.117243 0.096975 0.030701 0.088701 0.095597 0.093122 0.042602
Golgi 0.040844 0.069311 0.037853 0.017910 0.069058 0.081636 0.027219 0.011121 0.055556 0.075124 0.032961 0.014252
Lipid Particles 0.036715 0.011395 0.007440 0.006757 0.027486 0.027010 0.004699 0.006389 0.031903 0.018759 0.006179 0.006559
Mitochondria 0.117319* 0.139219* 0.103943* 0.013612 0.157222* 0.148229* 0.054061 0.013500 0.138126* 0.143468* 0.080996 0.013552
Mitotic Spindle 0.004128 0.023952 0.270503* 0.140501* 0.007549 0.015375 0.186868* 0.056638 0.005912 0.019907 0.232029* 0.095315
None 0.001647 0.001260 0.001453 0.001915 0.001601 0.002154 0.001726 0.002557 0.001623 0.001682 0.001578 0.002261
Nuclear Periphery 0.000568 0.000211 0.003332 0.000925 0.000364 0.000277 0.000844 0.001176 0.000462 0.000242 0.002187 0.001061
Nuclear Periphery Foci 0.000311 0.000319 0.003345 0.001593 0.000349 0.000305 0.000927 0.001509 0.000331 0.000313 0.002233 0.001548
Nucleolus 0.002222 0.001695 0.008706 0.002177 0.002780 0.003850 0.004357 0.003161 0.002513 0.002711 0.006705 0.002708
Nucleus 0.040501 0.023488 0.054546 0.060842 0.027424 0.019142 0.040334 0.128652 0.033682 0.021439 0.048008 0.097378
Peroxisomes 0.066846 0.163487* 0.105837* 0.212496* 0.073277 0.123262* 0.204700* 0.148792* 0.070200 0.144517* 0.151316* 0.178172*
Vacuole 0.038624 0.039882 0.064873 0.088431 0.045046 0.038916 0.057630 0.083284 0.041972 0.039427 0.061541 0.085658
Vacuole Periphery 0.005780 0.003840 0.005813 0.001503 0.007150 0.007479 0.002910 0.002344 0.006495 0.005556 0.004477 0.001956

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.74 -9.70 -6.13 -4.94 -2.11 -0.25 -6.10 -6.19 -6.32 -1.78 -5.73 -11.25 -8.45 -7.31 -2.52
Bud Neck -0.46 1.79 5.12 5.55 4.68 0.30 -2.39 2.24 1.88 5.42 -0.12 -0.35 4.92 4.98 6.63
Bud Site -9.63 -16.61 -14.69 -8.20 -5.66 -0.99 -14.74 -12.67 -11.71 -2.35 -7.99 -21.79 -19.25 -13.69 -5.72
Cell Periphery 1.01 4.71 1.24 0.02 -3.03 -5.44 0.09 -4.41 2.64 -4.19 -3.20 3.75 -1.82 1.81 -5.27
Cytoplasm 8.08 15.42 -1.65 -9.12 -15.56 4.66 0.59 -18.86 -22.88 -20.24 8.83 11.72 -15.09 -23.05 -25.98
Cytoplasmic Foci 11.07 40.02 32.27 22.55 -4.75 5.18 32.48 30.01 23.84 0.32 11.33 51.21 43.93 33.00 -3.24
Eisosomes 4.72 10.02 8.87 8.28 -4.52 -2.54 12.67 6.52 7.48 -7.97 2.13 14.69 10.88 10.56 -8.94
Endoplasmic Reticulum 2.93 -9.07 -5.27 -9.92 3.73 0.07 -5.90 -5.03 -4.13 -2.53 1.93 -11.28 -7.06 -8.02 0.66
Endosome 0.82 -0.62 7.65 7.24 9.55 -3.41 1.86 17.25 19.47 17.62 -1.70 1.12 17.77 19.41 19.41
Golgi -7.02 2.54 10.76 17.27 11.59 -2.55 13.31 18.40 19.38 10.08 -6.05 11.68 21.07 26.07 15.67
Lipid Particles 11.36 13.70 13.76 3.91 0.75 0.11 10.58 9.77 9.80 -1.15 7.27 16.93 16.47 10.40 -0.06
Mitochondria -2.55 3.32 18.89 21.83 24.39* 1.04 16.03 22.95 22.06 14.46* -0.75 13.45 29.61 31.03 28.10*
Mitotic Spindle -8.93 -42.70* -18.17 -14.53 14.85 -3.44 -30.96* -11.75 -8.93 19.13* -8.78 -52.12 -21.29 -16.60 24.39*
None 0.74 0.33 -0.90 -1.64 -1.42 -0.91 -0.52 -1.68 -0.33 -1.33 -0.33 -0.21 -1.90 -1.40 -2.11
Nuclear Periphery 3.22 -7.23 -4.94 -7.59 4.76 1.40 -9.42 -8.99 -9.61 -1.97 3.54 -8.85 -9.30 -12.14 4.10
Nuclear Periphery Foci -0.30 -7.10 -8.04 -7.91 1.99 0.63 -4.84 -3.24 -3.36 -0.83 0.33 -8.26 -6.95 -6.99 1.36
Nucleolus 1.48 -8.90 -2.18 -3.28 6.82 -1.16 -3.52 -0.70 0.46 2.82 -0.33 -8.69 -1.68 -1.42 6.94
Nucleus 5.06 -7.16 -8.83 -12.35 -3.16 3.11 -7.74 -21.64 -23.34 -16.13 5.69 -10.74 -22.20 -25.60 -14.25
Peroxisomes -14.88 -6.77 -11.49 0.87 -7.22 -8.21 -17.35 -5.73 1.62 9.33 -16.51 -17.16 -12.17 2.20 1.14
Vacuole -0.90 -11.62 -17.27 -16.61 -11.06 1.62 -8.33 -15.92 -16.88 -9.98 0.64 -13.88 -23.51 -23.67 -14.90
Vacuole Periphery 4.18 0.86 10.71 7.76 10.20 -0.38 7.13 6.94 7.07 1.61 2.00 5.73 10.86 9.16 6.95
Standard name
Human Ortholog
Description O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
Localization
Cell Percentages mitochondrion (56%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Coq3

Coq3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available