Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 2 | 1 | 1 | 0 | – | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 5 | 7 | 10 | 14 | 2 | 1 | 0 | 1 | 16 | 24 |
Bud | 0 | 0 | 1 | 2 | 8 | 5 | – | 13 | 7 | 15 | 7 | 15 | 2 | 3 | 1 | 2 | 1 | 4 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 0 | 1 | – | 7 | 5 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 3 | 0 | 1 | 0 | – | 4 | 1 | 3 | 4 | 6 | 1 | 0 | 3 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 2 | 0 | 5 | 7 | – | 34 | 8 | 23 | 13 | 13 | 1 | 2 | 7 | 4 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 1 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 2 | 6 | 8 |
Golgi | 10 | 6 | 2 | 1 | 19 | 1 | – | 0 | 0 | 1 | 1 | 2 | 4 | 13 | 37 | 24 | 20 | 43 | 17 | 8 | 6 | 12 | 48 | 57 |
Mitochondria | 365 | 85 | 102 | 218 | 346 | 246 | – | 357 | 359 | 492 | 275 | 552 | 167 | 328 | 410 | 4 | 7 | 7 | 348 | 80 | 86 | 80 | 206 | 250 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | – | 11 | 2 | 11 | 2 | 4 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 9 | 2 | 5 | 5 | 8 | 28 | – | 22 | 41 | 36 | 40 | 51 | 6 | 7 | 7 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 50 | 10 | 8 | 21 | 35 | 49 | – | 78 | 67 | 72 | 49 | 85 | 7 | 28 | 22 | 5 | 6 | 17 | 4 | 0 | 0 | 1 | 6 | 6 |
Peroxisomes | 0 | 0 | 0 | 2 | 0 | 1 | – | 5 | 1 | 3 | 0 | 3 | 3 | 5 | 6 | 2 | 1 | 5 | 1 | 0 | 3 | 1 | 13 | 11 |
SpindlePole | 1 | 0 | 0 | 1 | 0 | 0 | – | 2 | 3 | 1 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 38 | 11 | 6 | 4 | 18 | 5 | – | 16 | 18 | 21 | 5 | 30 | 30 | 33 | 39 | 6 | 13 | 15 | 13 | 6 | 5 | 12 | 21 | 25 |
Unique Cell Count | 395 | 99 | 106 | 222 | 372 | 273 | 446 | 399 | 561 | 302 | 609 | 192 | 363 | 456 | 52 | 59 | 103 | 398 | 102 | 106 | 114 | 329 | 402 | |
Labelled Cell Count | 480 | 118 | 133 | 257 | 442 | 345 | 552 | 514 | 686 | 403 | 775 | 222 | 422 | 539 | 63 | 67 | 127 | 398 | 102 | 106 | 114 | 329 | 402 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.2 | 23.6 | 10.8 | 13.9 | 14.9 | 12.1 | – | 10.2 | 9.8 | 9.8 | 9.1 | 9.1 | 19.0 | 18.9 | 18.4 | 25.7 | 28.0 | 22.8 | 20.9 | 20.5 | 21.1 |
Std Deviation (1e-4) | 3.9 | 5.5 | 2.1 | 3.0 | 3.1 | 3.7 | – | 3.6 | 3.0 | 2.7 | 2.9 | 2.5 | 4.6 | 4.2 | 4.2 | 8.0 | 9.4 | 8.1 | 9.8 | 5.1 | 5.6 |
Intensity Change (Log2) | – | – | – | 0.37 | 0.46 | 0.16 | – | -0.08 | -0.14 | -0.15 | -0.25 | -0.24 | 0.81 | 0.81 | 0.77 | 1.25 | 1.37 | 1.08 | 0.95 | 0.92 | 0.97 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 7.1 | 5.8 | 7.0 |
Mitochondria | 1.1 | -1.2 | -2.0 | – | -4.0 | -2.0 | -2.6 | -1.7 | -1.9 | -2.6 | -1.9 | -2.1 | -11.1 | -11.0 | -12.9 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.7 | – | 0.1 | 1.8 | 0.7 | 2.4 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.6 | 0.6 | 2.5 | – | 2.5 | 2.4 | 1.5 | 2.2 | 1.8 | -1.5 | 0.1 | -1.1 | 0 | 0.6 | 2.0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.3 | 0 | – | 0 | -0.5 | 0 | 0 | -0.3 | 2.5 | 1.1 | 1.0 | 0 | 3.2 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.3382 | 14.1558 | 13.3844 | 13.9158 | 10.4468 | 13.2667 | 14.661 | 14.6739 | 13.9518 | 13.8045 | 13.6804 | 14.0177 | 17.7806 | 17.2387 | 17.7349 | 17.227 | 15.0113 | 17.2529 |
Actin | 0.012 | 0.0058 | 0.0048 | 0.0257 | 0.0018 | 0.0087 | 0.0243 | 0.0037 | 0.0041 | 0.0092 | 0.002 | 0.0037 | 0.0133 | 0.0116 | 0.0051 | 0.0006 | 0.0172 | 0.0046 |
Bud | 0.0027 | 0.0059 | 0.001 | 0.009 | 0.0001 | 0.0004 | 0.0005 | 0.0044 | 0.0013 | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0017 | 0.0015 | 0.0007 | 0.0003 | 0.0006 |
Bud Neck | 0.0004 | 0.0007 | 0.0007 | 0.0007 | 0.0001 | 0.0019 | 0.0006 | 0.0019 | 0.0004 | 0.0005 | 0.0003 | 0.0006 | 0.0008 | 0.0006 | 0.0004 | 0.0008 | 0.0005 | 0.0091 |
Bud Periphery | 0.0022 | 0.0106 | 0.0018 | 0.0151 | 0.0003 | 0.0005 | 0.0008 | 0.0075 | 0.0012 | 0.0005 | 0.0001 | 0.0006 | 0.0011 | 0.0025 | 0.0018 | 0.0012 | 0.0003 | 0.0013 |
Bud Site | 0.0038 | 0.0301 | 0.0047 | 0.0045 | 0.0001 | 0.0008 | 0.0022 | 0.0239 | 0.0048 | 0.0004 | 0.0001 | 0.0004 | 0.0044 | 0.0058 | 0.0053 | 0.0021 | 0.0007 | 0.0006 |
Cell Periphery | 0.0007 | 0.0026 | 0.0004 | 0.0008 | 0.0002 | 0.0003 | 0.0009 | 0.0035 | 0.0013 | 0.0004 | 0.0002 | 0.0005 | 0.0009 | 0.0016 | 0.0006 | 0.0007 | 0.0004 | 0.0003 |
Cytoplasm | 0.0034 | 0.001 | 0.0005 | 0.0064 | 0.0001 | 0.0004 | 0.0012 | 0.0017 | 0.001 | 0.0001 | 0 | 0.0001 | 0.0036 | 0.0081 | 0.0003 | 0 | 0.0001 | 0.0028 |
Cytoplasmic Foci | 0.0167 | 0.0071 | 0.0125 | 0.0067 | 0.0043 | 0.0082 | 0.0094 | 0.0127 | 0.0088 | 0.0005 | 0.002 | 0.0056 | 0.0124 | 0.0075 | 0.0032 | 0.0037 | 0.0075 | 0.0036 |
Eisosomes | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0014 | 0.0003 | 0.0005 | 0.0033 | 0.0001 | 0.0005 | 0.001 | 0.0005 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0014 |
Endosome | 0.013 | 0.0072 | 0.0099 | 0.0146 | 0.006 | 0.0216 | 0.0216 | 0.0264 | 0.0078 | 0.0033 | 0.0064 | 0.0097 | 0.0147 | 0.0113 | 0.0045 | 0.001 | 0.0018 | 0.0062 |
Golgi | 0.0069 | 0.0173 | 0.0113 | 0.0041 | 0.0055 | 0.0302 | 0.0144 | 0.0188 | 0.0135 | 0.0069 | 0.0266 | 0.0263 | 0.0116 | 0.0148 | 0.012 | 0.0013 | 0.0122 | 0.0159 |
Lipid Particles | 0.0296 | 0.0081 | 0.0126 | 0.0022 | 0.0707 | 0.0133 | 0.0216 | 0.0107 | 0.0125 | 0.0033 | 0.0262 | 0.0131 | 0.0241 | 0.0224 | 0.0115 | 0.0111 | 0.0043 | 0.0048 |
Mitochondria | 0.8686 | 0.8728 | 0.9044 | 0.8945 | 0.8925 | 0.8941 | 0.8114 | 0.8008 | 0.8786 | 0.9589 | 0.9213 | 0.9223 | 0.8409 | 0.8175 | 0.9173 | 0.9527 | 0.9064 | 0.9222 |
None | 0.0026 | 0.0003 | 0.0004 | 0.0017 | 0.0002 | 0.0002 | 0.0015 | 0.0002 | 0.0003 | 0.0007 | 0 | 0 | 0.0028 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0061 | 0.0002 | 0.0008 | 0.002 | 0.0008 | 0.0005 | 0.0043 | 0.0011 | 0.001 | 0.002 | 0.0001 | 0 | 0.0013 | 0.0002 | 0.0001 | 0 | 0 | 0.0008 |
Nucleolus | 0.0008 | 0.0032 | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0136 | 0.0104 | 0.0123 | 0.0005 | 0.0005 | 0.0061 | 0.0074 | 0.0117 | 0.005 | 0.0129 | 0.035 | 0.0009 |
Nucleus | 0.0014 | 0.0008 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0019 | 0.0026 | 0.0024 | 0.0006 | 0.0001 | 0.0004 | 0.0027 | 0.0013 | 0.0008 | 0.0007 | 0.0017 | 0.0014 |
Peroxisomes | 0.0122 | 0.0069 | 0.0077 | 0.0017 | 0.0099 | 0.0057 | 0.0183 | 0.014 | 0.0067 | 0.0021 | 0.0029 | 0.0043 | 0.0161 | 0.02 | 0.008 | 0.006 | 0.0091 | 0.0088 |
Punctate Nuclear | 0.0031 | 0.0007 | 0.0024 | 0.002 | 0.0007 | 0.0011 | 0.0021 | 0.0002 | 0.0034 | 0.0003 | 0 | 0 | 0.0016 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0009 |
Vacuole | 0.0053 | 0.0087 | 0.0057 | 0.0015 | 0.0017 | 0.0033 | 0.0184 | 0.0283 | 0.0246 | 0.0034 | 0.0013 | 0.0024 | 0.019 | 0.0267 | 0.0111 | 0.0027 | 0.0009 | 0.0036 |
Vacuole Periphery | 0.0067 | 0.0095 | 0.0159 | 0.0024 | 0.0043 | 0.0078 | 0.0292 | 0.0263 | 0.0135 | 0.0054 | 0.0096 | 0.0033 | 0.0195 | 0.0343 | 0.0096 | 0.0014 | 0.0011 | 0.01 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.6501 | 41.647 | 47.1691 | 47.4728 | 30.2018 | 41.6611 | 38.2308 | 55.346 | 60.9035 | 57.4892 |
Translational Efficiency | 2.9933 | 2.1175 | 1.7319 | 2.2742 | 2.8046 | 2.4941 | 3.2196 | 2.2186 | 2.0505 | 2.03 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1392 | 1328 | 336 | 1354 | 1458 | 50 | 418 | 1626 | 2850 | 1378 | 754 | 2980 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1225.01 | 1576.63 | 1764.39 | 1958.56 | 1333.67 | 1693.92 | 1879.95 | 1944.22 | 1280.60 | 1580.89 | 1828.45 | 1950.74 |
Standard Deviation | 275.78 | 391.03 | 442.21 | 407.33 | 345.09 | 378.63 | 336.95 | 391.45 | 317.84 | 391.21 | 391.64 | 398.81 |
Intensity Change Log 2 | — | 0.364051 | 0.526376 | 0.677000 | — | 0.344964 | 0.495293 | 0.543790 | — | 0.354134 | 0.510258 | 0.609102 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001996 | 0.004584 | 0.014825 | 0.003257 | 0.002237 | 0.003378 | 0.005633 | 0.002609 | 0.002119 | 0.004541 | 0.009729 | 0.002903 |
Bud Neck | 0.002727 | 0.002668 | 0.015404 | 0.003331 | 0.000997 | 0.003916 | 0.007244 | 0.000492 | 0.001842 | 0.002714 | 0.010880 | 0.001782 |
Bud Site | 0.007809 | 0.006643 | 0.034553 | 0.004307 | 0.002829 | 0.005271 | 0.009876 | 0.000988 | 0.005261 | 0.006594 | 0.020873 | 0.002496 |
Cell Periphery | 0.000505 | 0.000209 | 0.000919 | 0.000666 | 0.001611 | 0.000419 | 0.000346 | 0.000575 | 0.001070 | 0.000216 | 0.000601 | 0.000616 |
Cytoplasm | 0.001592 | 0.000613 | 0.003058 | 0.000480 | 0.000248 | 0.000719 | 0.000810 | 0.000228 | 0.000904 | 0.000617 | 0.001812 | 0.000342 |
Cytoplasmic Foci | 0.026301 | 0.024373 | 0.059782 | 0.006038 | 0.005903 | 0.075143 | 0.031522 | 0.004039 | 0.015866 | 0.026215 | 0.044115 | 0.004947 |
Eisosomes | 0.000285 | 0.000549 | 0.000675 | 0.000480 | 0.001095 | 0.001044 | 0.000391 | 0.000462 | 0.000700 | 0.000567 | 0.000518 | 0.000470 |
Endoplasmic Reticulum | 0.000387 | 0.000182 | 0.008639 | 0.000232 | 0.000160 | 0.001259 | 0.000535 | 0.000116 | 0.000271 | 0.000221 | 0.004146 | 0.000169 |
Endosome | 0.019108 | 0.008507 | 0.039099 | 0.002456 | 0.001414 | 0.014712 | 0.010460 | 0.001350 | 0.010056 | 0.008732 | 0.023222 | 0.001853 |
Golgi | 0.027592 | 0.024712 | 0.051922 | 0.015923 | 0.004320 | 0.046871 | 0.022871 | 0.011212 | 0.015687 | 0.025516 | 0.035817 | 0.013352 |
Lipid Particles | 0.009117 | 0.006607 | 0.010521 | 0.003651 | 0.003251 | 0.020436 | 0.005695 | 0.000567 | 0.006116 | 0.007109 | 0.007846 | 0.001968 |
Mitochondria | 0.853484* | 0.883398* | 0.629822* | 0.895468* | 0.956363* | 0.721956* | 0.797372* | 0.958303* | 0.906115* | 0.877540* | 0.722708* | 0.929753* |
Mitotic Spindle | 0.001281 | 0.002345 | 0.020059 | 0.002505 | 0.000138 | 0.028642 | 0.021616 | 0.000506 | 0.000696 | 0.003300 | 0.020922 | 0.001414 |
None | 0.001961 | 0.002017 | 0.003113 | 0.002997 | 0.002958 | 0.001603 | 0.002444 | 0.001588 | 0.002471 | 0.002002 | 0.002742 | 0.002228 |
Nuclear Periphery | 0.000586 | 0.000436 | 0.002143 | 0.000753 | 0.000181 | 0.009385 | 0.002611 | 0.000092 | 0.000378 | 0.000760 | 0.002402 | 0.000392 |
Nuclear Periphery Foci | 0.000709 | 0.000373 | 0.002336 | 0.000372 | 0.000202 | 0.006297 | 0.001451 | 0.000055 | 0.000450 | 0.000588 | 0.001845 | 0.000199 |
Nucleolus | 0.001178 | 0.001069 | 0.002506 | 0.001110 | 0.000653 | 0.000925 | 0.001953 | 0.000177 | 0.000909 | 0.001064 | 0.002200 | 0.000601 |
Nucleus | 0.000365 | 0.000221 | 0.000531 | 0.002887 | 0.000096 | 0.000209 | 0.000220 | 0.000029 | 0.000228 | 0.000221 | 0.000358 | 0.001328 |
Peroxisomes | 0.013410 | 0.012008 | 0.013946 | 0.003671 | 0.005963 | 0.029634 | 0.013550 | 0.002381 | 0.009600 | 0.012647 | 0.013726 | 0.002967 |
Vacuole | 0.014000 | 0.010297 | 0.050131 | 0.016980 | 0.003928 | 0.018957 | 0.030224 | 0.003303 | 0.008848 | 0.010611 | 0.039095 | 0.009517 |
Vacuole Periphery | 0.015607 | 0.008189 | 0.036017 | 0.032435 | 0.005456 | 0.009225 | 0.033177 | 0.010930 | 0.010414 | 0.008227 | 0.034443 | 0.020701 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.47 | -5.33 | -1.60 | 1.25 | 4.61 | -1.16 | -2.25 | -0.43 | 0.69 | 1.90 | -3.33 | -5.58 | -1.35 | 1.91 | 4.77 |
Bud Neck | 0.12 | -7.36 | -1.31 | -1.42 | 6.62 | -2.30 | -5.93 | 2.28 | 2.72 | 6.51 | -2.08 | -9.24 | -0.27 | 1.70 | 9.01 |
Bud Site | 0.65 | -4.71 | 2.09 | 1.21 | 5.29 | -0.93 | -2.86 | 1.83 | 1.74 | 3.77 | -0.85 | -5.37 | 2.93 | 2.80 | 6.34 |
Cell Periphery | 2.09 | -2.54 | -0.87 | -3.26 | 1.63 | 1.74 | 1.97 | 1.60 | -0.50 | -1.21 | 2.58 | 1.28 | 1.30 | -3.65 | 0.11 |
Cytoplasm | 1.93 | -1.57 | 1.87 | -0.15 | 2.64 | -2.46 | -3.14 | 0.22 | 2.53 | 3.20 | 1.11 | -1.98 | 1.94 | 1.10 | 3.22 |
Cytoplasmic Foci | 0.73 | -5.23 | 9.71 | 9.17 | 8.59 | -4.15 | -6.11 | 2.02 | 4.26 | 6.60 | -4.72 | -7.55 | 9.60 | 10.81 | 10.72 |
Eisosomes | -5.80 | -4.09 | -4.12 | 1.19 | 1.92 | 0.12 | 4.70 | 3.89 | 1.41 | -0.59 | 1.67 | 2.04 | 2.60 | 1.39 | 0.59 |
Endoplasmic Reticulum | 3.07 | -2.87 | 1.54 | -1.53 | 2.91 | -1.36 | -1.01 | 1.27 | 1.41 | 1.13 | 1.04 | -2.97 | 2.07 | 0.90 | 3.03 |
Endosome | 4.81 | -3.90 | 8.69 | 5.06 | 7.67 | -2.40 | -5.35 | 0.13 | 2.41 | 5.39 | 0.90 | -5.17 | 8.35 | 5.99 | 8.98 |
Golgi | 0.93 | -3.81 | 4.20 | 3.25 | 5.84 | -2.78 | -5.11 | -4.47 | 2.32 | 3.06 | -4.02 | -5.68 | 1.49 | 5.09 | 6.41 |
Lipid Particles | 1.67 | -0.89 | 3.47 | 2.29 | 4.19 | -1.56 | -1.98 | 4.79 | 1.80 | 4.57 | -0.88 | -1.68 | 5.16 | 5.07 | 6.07 |
Mitochondria | -3.09 | 10.36 | -4.02 | -0.86 | -12.28 | 4.90 | 10.50 | -0.40 | -4.95 | -10.69 | 3.69 | 13.87 | -4.13 | -6.89 | -15.76 |
Mitotic Spindle | -1.24 | -5.26 | -2.25 | -0.52 | 4.78 | -1.40 | -4.43 | -2.83 | 1.38 | 4.35 | -2.40 | -6.53 | -2.64 | 1.54 | 6.23 |
None | -0.30 | -3.07 | -5.78 | -4.78 | 0.17 | 4.06 | 1.92 | 8.59 | 0.05 | 3.60 | 2.76 | -1.15 | 1.96 | -1.45 | 2.27 |
Nuclear Periphery | 1.04 | -4.58 | -1.48 | -2.30 | 4.07 | -1.28 | -3.82 | 2.35 | 1.30 | 3.96 | -1.33 | -5.27 | -0.31 | 1.27 | 5.23 |
Nuclear Periphery Foci | 2.55 | -3.79 | 2.45 | -0.01 | 4.68 | -1.28 | -2.62 | 2.89 | 1.31 | 2.94 | -0.68 | -4.27 | 3.52 | 2.03 | 5.10 |
Nucleolus | 0.21 | -2.10 | -0.25 | -0.53 | 2.17 | -0.83 | -2.82 | 3.13 | 2.57 | 4.05 | -0.43 | -3.33 | 1.00 | 1.14 | 4.31 |
Nucleus | 1.19 | -1.38 | -3.14 | -3.24 | -2.95 | -2.05 | -3.04 | 3.68 | 3.44 | 5.12 | 0.14 | -1.85 | -3.08 | -3.09 | -2.81 |
Peroxisomes | 0.67 | -0.19 | 5.72 | 5.47 | 4.81 | -1.74 | -2.75 | 2.77 | 2.01 | 4.45 | -1.76 | -2.15 | 6.25 | 6.63 | 6.37 |
Vacuole | 2.20 | -6.67 | -1.56 | -3.43 | 6.00 | -1.96 | -6.43 | 0.84 | 2.04 | 6.57 | -1.30 | -9.06 | -0.72 | 0.71 | 8.77 |
Vacuole Periphery | 4.39 | -4.53 | -5.73 | -9.20 | 0.73 | -1.59 | -6.44 | -3.27 | -0.63 | 4.95 | 1.94 | -7.68 | -6.24 | -7.74 | 4.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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