Standard name
Human Ortholog
Description Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.93 0.92 0.95 0.92 0.86 0.8 0.75 0.73 0.7 0.69 0.96 0.95 0.95 0.96 0.79 0.73 0.44 0.62 0.68 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0.19 0.19 0.27 0.28 0.33 0.24 0 0 0 0 0.05 0.07 0.11 0.12 0.09 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0 0.05 0 0 0 0.07 0.07 0.1 0 0 0 0 0.07 0.06 0.28 0.11 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 11 7 11 11 1 3 3 1 4 4 11 1 5 3
Bud 0 2 0 7 3 17 15 33 45 38 48 0 5 1 0 2 2 11 1 8 7
Bud Neck 3 1 1 2 2 3 3 3 4 0 3 0 2 2 2 4 0 9 2 2 5
Bud Site 0 0 0 0 0 0 1 1 2 1 4 0 0 1 0
Cell Periphery 2 6 4 9 4 5 11 22 27 20 24 3 11 11 6 1 3 4 2 3 4
Cytoplasm 341 161 455 485 607 611 693 539 679 555 650 77 187 211 281 275 121 216 61 151 183
Endoplasmic Reticulum 0 4 1 0 0 3 7 8 8 10 11 0 2 2 13 2 1 9 1 4 2
Endosome 0 0 2 3 11 4 18 10 9 6 9 0 0 0 1 2 1 16 1 2 4
Golgi 4 0 3 1 0 0 0 0 2 0 1 0 0 0 0 3 0 2 0 0 0
Mitochondria 5 16 7 18 16 131 162 196 260 261 229 0 0 0 8 17 12 55 12 20 11
Nucleus 0 0 3 2 3 2 8 4 7 11 20 1 1 0 3 1 0 0 0 0 0
Nuclear Periphery 0 0 5 1 4 0 7 9 14 13 13 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 4 1 1 2 5 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 3 7 2 6 0 7 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 5 7 11 14 18 20 15 26 0 0 1 0 1 0 5 0 1 4
Vac/Vac Membrane 4 1 40 13 35 15 28 23 66 59 95 0 0 1 9 25 10 139 10 18 24
Unique Cell Count 352 173 493 512 660 707 867 714 935 796 948 80 196 223 292 350 167 490 100 224 255
Labelled Cell Count 359 191 522 546 694 805 980 880 1157 1002 1156 82 211 233 324 350 167 490 100 224 255


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.2 4.0 3.9 4.1 3.3 3.6 3.5 3.6 3.8 4.0 5.1 5.3 5.4 4.1 4.6 5.4 4.8 5.7 6.0
Std Deviation (1e-4) 0.9 0.8 1.7 1.5 2.1 1.6 1.4 1.4 1.7 1.9 2.2 1.1 1.3 1.8 1.5 1.7 2.1 1.3 5.2 3.8
Intensity Change (Log2) -0.02 0.03 -0.26 -0.15 -0.21 -0.15 -0.06 -0.01 0.34 0.42 0.43 0.03 0.21 0.43 0.27 0.52 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole -2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0017 1.665 0.8846 0.3757 1.3208 1.0799 1.0586 1.2642 0.9237 0.7183 0.486 0.9344 1.1576 0.8466 0.7204 2.616 0.9674 0.8964
Actin 0.0032 0.0005 0.0077 0.0011 0.0022 0.0038 0.0504 0.0021 0.0108 0.0014 0.0058 0.0013 0.0825 0.001 0.0248 0.0002 0.0009 0.0005
Bud 0.0031 0.0045 0.008 0.0083 0.0055 0.0036 0.0051 0.0041 0.0028 0.0011 0.0008 0.009 0.0014 0.0023 0.0038 0.0015 0.0006 0.0006
Bud Neck 0.0014 0.0019 0.0096 0.0086 0.0079 0.0343 0.0214 0.0014 0.0065 0.0015 0.0002 0.004 0.0027 0.0006 0.0044 0.0017 0.0015 0.007
Bud Periphery 0.0152 0.0103 0.0395 0.0251 0.0644 0.0479 0.0165 0.0126 0.0137 0.0072 0.0011 0.0111 0.0022 0.006 0.0199 0.019 0.008 0.0042
Bud Site 0.0104 0.0523 0.0435 0.0034 0.0033 0.0031 0.0326 0.0513 0.0152 0.0012 0.0007 0.0011 0.0272 0.0107 0.0285 0.0066 0.0011 0.0006
Cell Periphery 0.1457 0.0954 0.0862 0.0587 0.1939 0.1234 0.013 0.0183 0.0284 0.0076 0.0005 0.0069 0.0621 0.0392 0.0544 0.1689 0.0843 0.0378
Cytoplasm 0.2448 0.2381 0.1903 0.2648 0.1397 0.301 0.0715 0.2312 0.1539 0.243 0.1501 0.1935 0.2139 0.2916 0.3146 0.1865 0.2441 0.3294
Cytoplasmic Foci 0.0142 0.0122 0.0142 0.0125 0.011 0.0162 0.0387 0.0137 0.0242 0.019 0.0259 0.0206 0.0396 0.0196 0.0144 0.0017 0.0091 0.0051
Eisosomes 0.0012 0.0002 0.0012 0.0003 0.0004 0.0004 0.0013 0.0008 0.0005 0.0003 0.0003 0.0002 0.0009 0.0001 0.0009 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0086 0.008 0.0111 0.0085 0.004 0.0084 0.0049 0.0085 0.0047 0.0045 0.0013 0.002 0.0054 0.01 0.017 0.002 0.003 0.0018
Endosome 0.0165 0.0311 0.0159 0.0305 0.0372 0.0125 0.0338 0.0395 0.0293 0.0542 0.0297 0.0214 0.0312 0.0387 0.0306 0.0134 0.0742 0.0114
Golgi 0.0012 0.0009 0.0009 0.0008 0.0017 0.0007 0.0147 0.0014 0.0032 0.0005 0.0029 0.0015 0.0092 0.0018 0.0019 0.0001 0.0023 0.0001
Lipid Particles 0.0059 0.0025 0.005 0.0038 0.0029 0.0026 0.0368 0.0029 0.0065 0.0022 0.0041 0.0123 0.0164 0.0099 0.0109 0.0008 0.0015 0.0008
Mitochondria 0.0028 0.0012 0.0018 0.001 0.0006 0.0008 0.0188 0.0021 0.0144 0.0013 0.0129 0.0031 0.0048 0.0061 0.0057 0.0002 0.0002 0.0001
None 0.4883 0.4465 0.4985 0.5269 0.5104 0.3967 0.5544 0.528 0.6052 0.539 0.6471 0.6186 0.3053 0.4644 0.392 0.4352 0.5012 0.5751
Nuclear Periphery 0.0013 0.0016 0.0019 0.0031 0.0007 0.0009 0.0198 0.0022 0.0026 0.0027 0.0016 0.0009 0.0025 0.0013 0.0013 0.0013 0.0005 0.0002
Nucleolus 0.0011 0.0006 0.0008 0.001 0.0003 0.0004 0.0034 0.0018 0.001 0.0006 0.0007 0.0004 0.0011 0.0007 0.0009 0.0006 0.0001 0.0001
Nucleus 0.001 0.0024 0.004 0.0037 0.0011 0.0012 0.0048 0.0029 0.0025 0.0032 0.0013 0.0015 0.0017 0.0018 0.001 0.0025 0.0004 0.0003
Peroxisomes 0.0006 0.0001 0.0007 0.0005 0.0002 0.0002 0.0209 0.0007 0.0067 0.0003 0.008 0.0014 0.0342 0.0003 0.0016 0 0.0001 0
Punctate Nuclear 0.0005 0.0004 0.0007 0.0033 0.0004 0.0004 0.0081 0.0012 0.0017 0.0011 0.0023 0.0011 0.0405 0.0004 0.0007 0.0001 0.0001 0.0001
Vacuole 0.032 0.0856 0.0566 0.0319 0.0117 0.04 0.0249 0.0682 0.056 0.1 0.0967 0.0827 0.1113 0.0885 0.0543 0.1493 0.0622 0.0241
Vacuole Periphery 0.0011 0.0037 0.0019 0.0022 0.0004 0.0015 0.0041 0.0052 0.0102 0.008 0.0059 0.0053 0.0038 0.0052 0.0163 0.0084 0.0048 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.2246 0.6629 0.4919 1.0326 0.4807 2.5939 1.1729 1.244 1.5349 1.2418
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (20%), vacuole (5%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Phm7

Phm7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Phm7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available