Standard name
Description Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.93 0.92 0.95 0.92 0.86 0.80 0.75 0.73 0.70 0.69 0.96 0.95 0.95 0.96 0.79 0.73 0.44 0.62 0.68 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0.19 0.19 0.27 0.28 0.33 0.24 0 0 0 0 0.05 0.07 0.11 0.12 0.09 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0 0.05 0 0 0 0.07 0.07 0.10 0 0 0 0 0.07 0.06 0.28 0.11 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 11 7 11 11 1 3 3 1 4 4 11 1 5 3
Bud 0 2 0 7 3 17 15 33 45 38 48 0 5 1 0 2 2 11 1 8 7
Bud Neck 3 1 1 2 2 3 3 3 4 0 3 0 2 2 2 4 0 9 2 2 5
Bud Site 0 0 0 0 0 0 1 1 2 1 4 0 0 1 0
Cell Periphery 2 6 4 9 4 5 11 22 27 20 24 3 11 11 6 1 3 4 2 3 4
Cytoplasm 341 161 455 485 607 611 693 539 679 555 650 77 187 211 281 275 121 216 61 151 183
Endoplasmic Reticulum 0 4 1 0 0 3 7 8 8 10 11 0 2 2 13 2 1 9 1 4 2
Endosome 0 0 2 3 11 4 18 10 9 6 9 0 0 0 1 2 1 16 1 2 4
Golgi 4 0 3 1 0 0 0 0 2 0 1 0 0 0 0 3 0 2 0 0 0
Mitochondria 5 16 7 18 16 131 162 196 260 261 229 0 0 0 8 17 12 55 12 20 11
Nucleus 0 0 3 2 3 2 8 4 7 11 20 1 1 0 3 1 0 0 0 0 0
Nuclear Periphery 0 0 5 1 4 0 7 9 14 13 13 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 4 1 1 2 5 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 3 7 2 6 0 7 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 5 7 11 14 18 20 15 26 0 0 1 0 1 0 5 0 1 4
Vac/Vac Membrane 4 1 40 13 35 15 28 23 66 59 95 0 0 1 9 25 10 139 10 18 24
Unique Cell Count 352 173 493 512 660 707 867 714 935 796 948 80 196 223 292 350 167 490 100 224 255
Labelled Cell Count 359 191 522 546 694 805 980 880 1157 1002 1156 82 211 233 324 350 167 490 100 224 255

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cell Periphery, Vacuole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.2 4.0 3.9 4.1 3.3 3.6 3.5 3.6 3.8 4.0 5.1 5.3 5.4 4.1 4.6 5.4 4.8 5.7 6.0
Std Deviation (1e-4) 0.9 0.8 1.7 1.5 2.1 1.6 1.4 1.4 1.7 1.9 2.2 1.1 1.3 1.8 1.5 1.7 2.1 1.3 5.2 3.8
Intensity Change (Log2) -0.02 0.03 -0.26 -0.15 -0.21 -0.15 -0.06 -0.01 0.34 0.42 0.43 0.03 0.21 0.43 0.27 0.52 0.58

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 0