Standard name
Human Ortholog
Description NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and meiotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0.05 0 0.05 0 0 0.05 0 0.11 0.14 0.2 0.06 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.41 0.21 0.43 0.41 0.37 0.62 0.74 0.67 0.81 0.84 0.79 0.85 0.06 0 0.06 0.09 0.08 0.13 0 0 0 0 0 0
Nucleus 0.91 0.9 0.83 0.84 0.83 0.73 0.63 0.65 0.6 0.57 0.54 0.5 0.83 0.81 0.73 0.9 0.8 0.88 0.85 0.88 0.75 0.79 0.67 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.06 0.06 0.05 0 0 0 0.06 0 0 0 0 0.09 0.08 0.11 0.05 0.08 0.1 0.07 0 0.1 0.13 0.18 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 0 1 1 1 0 3 3 3 5 1 3 0 1 0 0 1 0 0 2 1 0 1 3
Bud Neck 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 1 0 1 1 6 7 9 8 16 5 7 1 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 10 9 7 14 13 28 10 14 11 11 23 48 80 14 11 5 0 0 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 4 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 1 0 0 0 0 1 3 4 1 3 1 0 1 5 0 5 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 1 0 0 0
Mitochondria 37 52 93 91 91 165 271 364 272 449 163 230 12 15 25 22 10 13 1 2 1 0 1 0
Nucleus 82 227 181 185 207 194 230 355 203 303 112 136 176 279 286 213 104 86 77 225 162 105 157 154
Nuclear Periphery 3 2 0 4 1 0 2 1 0 6 5 5 1 1 3 2 1 0 0 0 0 0 0 1
Nucleolus 17 15 14 11 7 9 11 31 11 22 8 5 20 27 43 13 11 10 6 11 22 17 42 41
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 4 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 2 2 1 0 9 17 5 4 2 0 1 2 2 3 0 0 0 1 2 1 4 6
Vac/Vac Membrane 2 5 3 0 0 2 3 4 2 4 1 2 4 3 4 3 2 2 3 7 17 4 16 22
Unique Cell Count 90 253 218 221 248 264 364 542 336 532 206 271 211 344 394 237 130 98 91 255 218 134 235 244
Labelled Cell Count 141 308 306 304 317 390 550 814 514 823 308 399 239 386 449 278 148 118 91 255 218 134 235 244


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.3 4.2 4.2 4.1 3.6 3.2 3.4 3.2 2.9 3.1 2.9 6.0 5.9 5.4 6.4 7.5 8.0 5.5 5.7 6.4
Std Deviation (1e-4) 0.8 0.9 0.9 1.0 0.9 1.0 0.9 0.9 0.8 0.5 0.8 0.7 1.7 1.7 2.0 1.7 1.9 1.8 1.3 1.5 1.9
Intensity Change (Log2) – – – -0.03 -0.05 -0.22 -0.41 -0.3 -0.38 -0.52 -0.44 -0.52 0.51 0.48 0.36 0.61 0.82 0.91 0.37 0.43 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.0 0.4 -0.6 0.3 -1.0 -1.4 0.4 -0.3 2.5 3.6 5.3 0.6 1.5 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -8.9 -11.2 -10.9 -8.2 -6.9 -5.1
Nucleus 0.2 0.1 -2.5 -5.1 -4.8 -5.6 -6.8 -6.4 -7.6 0.1 -0.6 -2.9 2.1 -0.7 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1.2 0.6 1.8 -0.4 0.7 1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3709 3.5175 3.3166 2.9178 2.3196 3.0952 2.1731 1.9854 2.163 2.1505 1.9334 2.2169 3.1693 3.7004 3.1769 3.4544 2.6173 3.2618
Actin 0.0297 0 0.0051 0 0 0 0.0452 0 0.0066 0.0001 0.0001 0.0002 0.0108 0 0.0019 0.0037 0.0005 0.0022
Bud 0.001 0.0001 0.0011 0 0.0001 0 0.0002 0 0.0007 0 0 0 0.0019 0 0.0001 0.0001 0 0.0001
Bud Neck 0.0014 0.0002 0.0006 0.0001 0.001 0.0008 0.0003 0.0001 0.0001 0 0.0008 0.0007 0.0035 0.0001 0.0001 0.0008 0.0007 0.0009
Bud Periphery 0.0042 0.0003 0.0026 0 0.0002 0 0.0002 0 0.0012 0 0.0001 0 0.0048 0 0.0002 0.0002 0.0001 0.0001
Bud Site 0.0012 0.0005 0.0026 0 0.0002 0.0001 0.0015 0 0.0024 0 0.0001 0.0001 0.0043 0 0.0003 0.0034 0.0001 0.0001
Cell Periphery 0.002 0.0006 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0015 0 0 0.0001 0 0.0001
Cytoplasm 0.0029 0.0005 0.0041 0 0 0 0.0076 0.0006 0.001 0 0.0001 0.0001 0.0034 0 0.0002 0.0008 0.0002 0.0002
Cytoplasmic Foci 0.002 0 0.0033 0 0 0 0.0133 0 0.0044 0.0008 0.0071 0.0023 0.0332 0 0.0013 0.0188 0.0001 0.0016
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0022 0 0.0052 0 0 0 0.0023 0 0.0014 0 0 0 0.0038 0 0.0001 0.0001 0.0002 0
Endosome 0.021 0.0001 0.0056 0 0.0001 0 0.0107 0.0001 0.0073 0.0077 0.0023 0.0028 0.0149 0 0.001 0.0061 0.0009 0.0004
Golgi 0.0042 0 0.0058 0 0 0 0.0032 0 0.0032 0.007 0.0009 0.0048 0.0052 0 0.002 0.0035 0.0001 0.0007
Lipid Particles 0.0063 0 0.0025 0 0 0 0.0164 0 0.0018 0.0008 0.0208 0.0003 0.0249 0 0.0027 0.0108 0.0004 0.001
Mitochondria 0.0043 0.0003 0.024 0.0001 0.0012 0.0001 0.001 0.0001 0.0025 0.018 0.0004 0.0007 0.0137 0.0001 0.0354 0.0022 0.0005 0.0002
None 0.0029 0.0001 0.003 0 0.0001 0 0.011 0.0087 0.0005 0 0.0001 0 0.0018 0 0.0002 0.0006 0.0004 0.0001
Nuclear Periphery 0.0259 0.0026 0.0052 0.0008 0.0009 0.0001 0.0045 0.0012 0.0009 0.0003 0.002 0.0012 0.031 0.0001 0.0041 0.0008 0.0695 0.0004
Nucleolus 0.0286 0.0596 0.0245 0.0196 0.126 0.0765 0.0234 0.0218 0.0156 0.0119 0.1129 0.0607 0.1127 0.0462 0.0232 0.0264 0.1312 0.0761
Nucleus 0.8278 0.9307 0.8914 0.9782 0.8657 0.9183 0.8201 0.963 0.9397 0.952 0.8375 0.9246 0.5625 0.953 0.914 0.9043 0.7783 0.9035
Peroxisomes 0.0008 0 0.0022 0 0 0 0.0272 0 0.0086 0.0005 0.0049 0.0004 0.0522 0 0.0051 0.0154 0.0001 0.0111
Punctate Nuclear 0.0114 0.0015 0.0042 0.001 0.0037 0.004 0.0104 0.0037 0.0009 0.0001 0.0094 0.0009 0.0255 0.0004 0.0077 0.0012 0.0158 0.0009
Vacuole 0.015 0.0026 0.0057 0 0.0003 0.0001 0.0007 0.0003 0.0008 0.0003 0.0001 0.0001 0.0688 0 0.0002 0.0004 0.0002 0.0002
Vacuole Periphery 0.005 0.0002 0.0011 0 0.0002 0 0.0004 0.0001 0.0003 0.0003 0.0001 0 0.0194 0 0.0002 0.0002 0.0008 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.0959 32.6183 31.9495 34.8795 28.8582 38.6561 38.5869 41.4026 33.3473 38.0608
Translational Efficiency 0.6286 0.4473 0.2121 0.1973 0.4238 0.2922 0.297 0.1993 0.1598 0.257

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2052 893 310 2106 1532 1104 419 1917 3584 1997 729 4023

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.78 755.93 1056.66 946.00 709.77 775.15 1065.75 968.55 672.56 766.56 1061.88 956.75
Standard Deviation 77.44 154.94 137.69 140.35 95.37 126.82 131.59 144.93 91.40 140.42 134.29 143.00
Intensity Change Log 2 — 0.229446 0.712632 0.553033 — 0.127124 0.586446 0.448475 — 0.176736 0.647890 0.499192

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000074 0.000773 0.001876 0.001038 0.000166 0.000581 0.001720 0.000954 0.000113 0.000667 0.001786 0.000998
Bud Neck 0.002586 0.010367 0.013722 0.052136 0.003474 0.005896 0.015524 0.032092 0.002966 0.007896 0.014757 0.042585
Bud Site 0.000670 0.001945 0.001988 0.004527 0.000351 0.000860 0.002165 0.007426 0.000534 0.001345 0.002089 0.005908
Cell Periphery 0.000102 0.000190 0.000061 0.000124 0.000048 0.000099 0.000068 0.000167 0.000079 0.000140 0.000065 0.000144
Cytoplasm 0.019905 0.014560 0.000553 0.003664 0.006533 0.007923 0.003183 0.011160 0.014189 0.010891 0.002065 0.007236
Cytoplasmic Foci 0.001932 0.001895 0.000077 0.001916 0.000970 0.003215 0.000088 0.000539 0.001521 0.002625 0.000084 0.001260
Eisosomes 0.000015 0.000037 0.000053 0.000033 0.000022 0.000031 0.000059 0.000032 0.000018 0.000034 0.000057 0.000033
Endoplasmic Reticulum 0.000695 0.003859 0.006273 0.003962 0.001265 0.003266 0.006441 0.003179 0.000939 0.003531 0.006369 0.003589
Endosome 0.000232 0.001004 0.000232 0.002109 0.000267 0.001338 0.000286 0.001444 0.000247 0.001189 0.000263 0.001792
Golgi 0.000116 0.000647 0.000228 0.001562 0.000252 0.001891 0.000202 0.001090 0.000174 0.001335 0.000213 0.001337
Lipid Particles 0.000387 0.001665 0.000209 0.000753 0.000690 0.001528 0.000223 0.000102 0.000517 0.001589 0.000217 0.000443
Mitochondria 0.001835 0.002975 0.001399 0.004196 0.000526 0.003046 0.001409 0.002553 0.001276 0.003014 0.001405 0.003413
Mitotic Spindle 0.000614 0.003971 0.000635 0.016888 0.001028 0.005489 0.001962 0.014979 0.000791 0.004810 0.001398 0.015978
None 0.015889 0.024465 0.001433 0.003583 0.009469 0.010710 0.003204 0.009938 0.013145 0.016861 0.002451 0.006611
Nuclear Periphery 0.000509 0.000778 0.000250 0.001883 0.000337 0.000541 0.000394 0.000812 0.000435 0.000647 0.000333 0.001373
Nuclear Periphery Foci 0.000290 0.001964 0.000371 0.000986 0.000319 0.000685 0.000346 0.000397 0.000302 0.001257 0.000357 0.000706
Nucleolus 0.028380 0.056084 0.049956 0.015462 0.041707 0.050152 0.040453 0.019608 0.034076 0.052804 0.044494 0.017438
Nucleus 0.924771* 0.870001* 0.917376* 0.879632* 0.931890* 0.899969* 0.918571* 0.889269* 0.927814* 0.886568* 0.918063* 0.884224*
Peroxisomes 0.000276 0.001227 0.001343 0.000523 0.000307 0.001550 0.000908 0.000340 0.000289 0.001405 0.001093 0.000436
Vacuole 0.000516 0.001083 0.001758 0.003704 0.000275 0.000781 0.002595 0.003561 0.000413 0.000916 0.002239 0.003636
Vacuole Periphery 0.000207 0.000509 0.000205 0.001316 0.000104 0.000449 0.000200 0.000358 0.000163 0.000476 0.000202 0.000860

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.28 -15.41 -12.32 -3.29 5.25 -5.14 -17.96 -11.56 -4.57 8.26 -10.91 -23.78 -16.96 -5.64 9.67
Bud Neck -7.89 -10.38 -27.11 -20.90 -17.28 -3.54 -11.67 -22.83 -20.13 -7.53 -8.60 -15.52 -35.05 -28.84 -17.45
Bud Site -2.90 -5.05 -9.23 -4.74 -4.11 -3.74 -12.02 -7.66 -6.90 -3.09 -3.66 -11.02 -11.25 -8.77 -4.63
Cell Periphery -2.16 1.10 -0.19 2.69 -3.71 -3.50 -5.19 -8.26 -2.27 -3.93 -2.71 -0.14 -2.36 1.16 -5.05
Cytoplasm 1.76 10.90 8.82 4.68 -7.22 -0.76 2.36 -2.20 -1.12 -5.16 1.77 9.53 5.80 2.97 -6.32
Cytoplasmic Foci -0.54 4.68 2.19 2.39 -2.55 -2.36 3.37 2.45 3.37 -2.39 -1.95 5.66 3.10 3.99 -3.05
Eisosomes -8.23 -12.50 -16.52 1.37 5.69 -1.99 -8.75 -3.08 -1.87 9.92 -6.57 -14.83 -8.50 -0.27 11.34
Endoplasmic Reticulum -13.95 -8.11 -21.74 -2.43 2.10 -7.63 -14.93 -17.07 -2.63 8.31 -14.63 -16.27 -27.77 -3.73 7.10
Endosome -3.37 -0.63 -3.16 -0.67 -3.07 -1.95 -2.41 -2.76 0.62 -1.90 -2.96 -2.81 -4.18 0.14 -3.50
Golgi -1.49 -3.13 -2.60 -0.56 -2.15 -2.27 -0.20 -1.14 1.63 -1.16 -2.72 -1.53 -2.71 1.16 -2.36
Lipid Particles -1.81 3.30 -0.21 1.67 -1.03 -1.18 1.11 1.66 2.34 3.34 -2.23 1.68 1.20 2.74 -0.20
Mitochondria -1.38 0.54 -3.27 -1.79 -6.29 -3.10 -14.25 -6.84 0.54 -2.51 -2.81 -1.16 -5.01 -0.75 -6.53
Mitotic Spindle -2.18 -1.63 -8.26 -5.34 -7.90 -2.36 -2.51 -8.22 -5.20 -6.69 -3.27 -3.11 -11.70 -7.32 -9.94
None -2.29 12.76 10.82 6.63 -6.51 -0.78 5.16 0.71 1.41 -6.15 -1.81 12.63 8.31 6.45 -7.34
Nuclear Periphery -1.14 0.83 -5.60 -4.72 -8.12 -2.08 -4.21 -8.26 -5.65 -4.88 -1.55 -0.92 -7.75 -6.94 -9.07
Nuclear Periphery Foci -1.69 -0.43 -1.43 1.20 -1.30 -1.51 -0.99 -0.75 1.07 0.40 -2.03 -1.06 -1.61 1.34 -0.89
Nucleolus -8.51 -5.18 4.87 11.96 7.10 -2.36 -0.96 8.26 9.98 5.83 -7.93 -4.63 9.20 15.45 9.11
Nucleus 7.47 2.48 9.71 -0.29 5.14 5.09 5.21 9.87 3.30 2.65 8.59 5.41 13.67 2.32 5.32
Peroxisomes -2.89 -6.80 -0.40 2.63 4.98 -2.92 -6.07 0.62 3.05 7.54 -4.07 -8.98 -0.12 3.97 7.50
Vacuole -2.38 -4.49 -6.25 -4.91 -2.09 -0.84 -3.32 -4.71 -4.57 0.33 -2.20 -4.14 -7.21 -6.52 -0.07
Vacuole Periphery -2.73 -0.45 -3.46 -1.71 -3.51 -3.15 -7.44 -3.14 1.39 -0.67 -3.84 -1.95 -4.07 -0.83 -3.55
Standard name
Human Ortholog
Description NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and meiotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hst1

Hst1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hst1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available