Standard name | |
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Human Ortholog | |
Description | Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 1 | 3 | 0 | 3 | 4 | – | 2 | 6 | 5 | 8 | 2 | 0 | 1 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 149 | 292 | 202 | 216 | 206 | 205 | 266 | – | 184 | 247 | 154 | 194 | 168 | 290 | 246 | 17 | – | – | 149 | 277 | 199 | 158 | 164 | 269 |
Endoplasmic Reticulum | 0 | 3 | 2 | 1 | 3 | 2 | 2 | – | 1 | 4 | 3 | 5 | 1 | 1 | 4 | 0 | – | – | 0 | 0 | 3 | 0 | 2 | 2 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 0 | 1 |
Golgi | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 1 | 1 | 0 | 1 | 1 | 0 |
Mitochondria | 1 | 1 | 0 | 0 | 0 | 4 | 10 | – | 22 | 21 | 70 | 58 | 0 | 0 | 1 | 0 | – | – | 1 | 1 | 2 | 1 | 1 | 0 |
Nucleus | 0 | 2 | 0 | 2 | 2 | 3 | 7 | – | 10 | 15 | 7 | 9 | 0 | 1 | 2 | 0 | – | – | 0 | 0 | 2 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 10 | 5 | 11 | 11 | 26 | – | 14 | 58 | 20 | 32 | 0 | 0 | 2 | 1 | – | – | 1 | 1 | 3 | 0 | 0 | 2 |
Unique Cell Count | 151 | 297 | 210 | 222 | 219 | 218 | 304 | 221 | 331 | 228 | 277 | 169 | 293 | 249 | 18 | 155 | 291 | 221 | 168 | 177 | 286 | |||
Labelled Cell Count | 151 | 304 | 216 | 228 | 222 | 229 | 317 | 234 | 357 | 263 | 307 | 171 | 293 | 256 | 19 | 155 | 291 | 221 | 168 | 177 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 12.0 | 9.7 | 10.0 | 9.5 | 8.4 | 8.5 | – | 7.4 | 7.5 | 6.7 | 6.9 | 11.9 | 11.7 | 11.5 | 13.7 | – | – | 8.9 | 9.5 | 9.8 |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.3 | 1.8 | 1.3 | 1.3 | 1.8 | – | 1.4 | 1.4 | 1.4 | 1.3 | 1.8 | 2.1 | 2.0 | 2.7 | – | – | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.04 | -0.21 | -0.19 | – | -0.39 | -0.38 | -0.54 | -0.49 | 0.29 | 0.27 | 0.25 | 0.5 | – | – | -0.12 | -0.03 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | 0.6 | -1.0 | -1.0 | -3.4 | – | -4.4 | -6.5 | -7.7 | -7.3 | 2.0 | 2.1 | 1.8 | -0.4 | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.7 | 3.7 | 8.8 | 7.1 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | -1.4 | 0.1 | 0.1 | 1.7 | – | 0.7 | 4.4 | 1.7 | 2.6 | 0 | 0 | -2.6 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9155 | 5.7499 | 4.7794 | 4.0589 | 4.3647 | 5.1927 | 4.8577 | 6.2722 | 5.614 | 5.6478 | 4.9982 | 5.7482 | 5.8298 | 7.8108 | 6.6123 | 6.4106 | 7.1141 | 7.0002 |
Actin | 0.0387 | 0.0001 | 0.0083 | 0.0416 | 0.0002 | 0.0003 | 0.007 | 0.0005 | 0.011 | 0.0241 | 0.0085 | 0.0021 | 0.0336 | 0.0006 | 0.0124 | 0.0107 | 0.0322 | 0.0013 |
Bud | 0.0005 | 0.0001 | 0.0021 | 0.0009 | 0.0004 | 0.0001 | 0.0003 | 0.0027 | 0.0004 | 0.0004 | 0.0015 | 0.0016 | 0.0004 | 0.0017 | 0.0002 | 0.0005 | 0.0002 | 0.0001 |
Bud Neck | 0.0007 | 0.0001 | 0.0015 | 0.0006 | 0.0007 | 0.0007 | 0.0058 | 0.0002 | 0.0008 | 0.0008 | 0.0011 | 0.0011 | 0.0013 | 0.0001 | 0.0005 | 0.0008 | 0.0005 | 0.0008 |
Bud Periphery | 0.0008 | 0 | 0.0022 | 0.0011 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Bud Site | 0.0014 | 0.0002 | 0.0021 | 0.0025 | 0.0003 | 0.0002 | 0.0014 | 0.003 | 0.0014 | 0.0006 | 0.0018 | 0.0004 | 0.0009 | 0.0033 | 0.001 | 0.003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.8271 | 0.9851 | 0.9047 | 0.8211 | 0.867 | 0.9187 | 0.8641 | 0.9564 | 0.8927 | 0.8789 | 0.8823 | 0.9478 | 0.8317 | 0.9753 | 0.951 | 0.9235 | 0.9435 | 0.9711 |
Cytoplasmic Foci | 0.0214 | 0.0015 | 0.0124 | 0.014 | 0.0123 | 0.0041 | 0.0263 | 0.0063 | 0.0192 | 0.0181 | 0.0198 | 0.0117 | 0.034 | 0.0048 | 0.006 | 0.0047 | 0.0046 | 0.0039 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0007 | 0.0056 | 0.0011 | 0.001 | 0.0022 | 0.0033 | 0.001 | 0.0019 | 0.0013 | 0.0011 | 0.0014 | 0.0064 | 0.0005 | 0.0036 | 0.0054 | 0.0015 | 0.0014 |
Endosome | 0.0179 | 0.0008 | 0.0115 | 0.0202 | 0.0867 | 0.021 | 0.0276 | 0.0061 | 0.0151 | 0.0142 | 0.0308 | 0.0063 | 0.0191 | 0.0008 | 0.0056 | 0.0164 | 0.0017 | 0.0041 |
Golgi | 0.0162 | 0.0001 | 0.0054 | 0.0195 | 0.0171 | 0.0052 | 0.0102 | 0.0025 | 0.0052 | 0.0154 | 0.0267 | 0.0021 | 0.0077 | 0.0001 | 0.0026 | 0.0011 | 0.0008 | 0.0003 |
Lipid Particles | 0.0098 | 0 | 0.0078 | 0.0045 | 0.0014 | 0.0005 | 0.0052 | 0.0001 | 0.0017 | 0.0033 | 0.0044 | 0.0033 | 0.0104 | 0.0001 | 0.0033 | 0.0014 | 0.0001 | 0.0001 |
Mitochondria | 0.0207 | 0 | 0.0222 | 0.0535 | 0.0016 | 0.0074 | 0.0021 | 0.0074 | 0.0055 | 0.005 | 0.0031 | 0.0016 | 0.0034 | 0.0008 | 0.0007 | 0.0009 | 0.0003 | 0.0006 |
None | 0.0172 | 0.0091 | 0.0035 | 0.0043 | 0.0019 | 0.0257 | 0.0124 | 0.0065 | 0.0187 | 0.0069 | 0.0019 | 0.0054 | 0.014 | 0.0034 | 0.0035 | 0.0036 | 0.0062 | 0.0017 |
Nuclear Periphery | 0.0022 | 0.0003 | 0.0014 | 0.0007 | 0.0006 | 0.0016 | 0.0106 | 0.0007 | 0.0025 | 0.0012 | 0.0009 | 0.001 | 0.0093 | 0.001 | 0.0011 | 0.0073 | 0.0009 | 0.0031 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0007 | 0 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 |
Nucleus | 0.0022 | 0.0014 | 0.0023 | 0.003 | 0.0029 | 0.0063 | 0.0069 | 0.004 | 0.0097 | 0.0206 | 0.0079 | 0.0068 | 0.0095 | 0.0053 | 0.004 | 0.0094 | 0.0057 | 0.0092 |
Peroxisomes | 0.0142 | 0 | 0.002 | 0.0084 | 0.0004 | 0.0001 | 0.0057 | 0.0006 | 0.0053 | 0.0054 | 0.0055 | 0.0015 | 0.0117 | 0.0001 | 0.0027 | 0.0004 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.0001 | 0.0003 | 0.0004 | 0.0006 | 0.0009 | 0.0036 | 0.0007 | 0.0047 | 0.0023 | 0.0007 | 0.0043 | 0.0034 | 0.001 | 0.0006 | 0.0053 | 0.0002 | 0.0011 |
Vacuole | 0.0016 | 0.0003 | 0.0031 | 0.0014 | 0.0031 | 0.0034 | 0.0045 | 0.0005 | 0.0022 | 0.0009 | 0.0009 | 0.0006 | 0.0012 | 0.0004 | 0.0006 | 0.0036 | 0.0006 | 0.0006 |
Vacuole Periphery | 0.001 | 0 | 0.001 | 0.0006 | 0.0016 | 0.0012 | 0.0017 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.376 | 44.5491 | 62.5216 | 70.589 | 35.1157 | 37.3553 | 49.2052 | 58.9853 | 58.2161 | 39.7707 |
Translational Efficiency | 0.4673 | 0.5183 | 0.5405 | 0.5348 | 0.4205 | 0.5181 | 0.5448 | 0.3716 | 0.4599 | 0.3863 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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