Standard name
Description Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.97 0.94 0.94 0.88 0.83 0.75 0.68 0.7 0.99 0.99 0.99 0.94 0.96 0.95 0.9 0.94 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.1 0.06 0.31 0.21 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.05 0.05 0.09 0.06 0.18 0.09 0.12 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 2 0 0 0 1
Bud 0 0 1 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 4 1 3 0 3 4 2 6 5 8 2 0 1 0 0 0 0 0 0 0
Cytoplasm 149 292 202 216 206 205 266 184 247 154 194 168 290 246 17 149 277 199 158 164 269
Endoplasmic Reticulum 0 3 2 1 3 2 2 1 4 3 5 1 1 4 0 0 0 3 0 2 2
Endosome 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Golgi 1 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 0 1 1 0
Mitochondria 1 1 0 0 0 4 10 22 21 70 58 0 0 1 0 1 1 2 1 1 0
Nucleus 0 2 0 2 2 3 7 10 15 7 9 0 1 2 0 0 0 2 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 1 10 5 11 11 26 14 58 20 32 0 0 2 1 1 1 3 0 0 2
Unique Cell Count 151 297 210 222 219 218 304 221 331 228 277 169 293 249 18 155 291 221 168 177 286
Labelled Cell Count 151 304 216 228 222 229 317 234 357 263 307 171 293 256 19 155 291 221 168 177 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 12.0 9.7 10.0 9.5 8.4 8.5 7.4 7.5 6.7 6.9 11.9 11.7 11.5 13.7 8.9 9.5 9.8
Std Deviation (1e-4) 1.3 1.7 1.3 1.8 1.3 1.3 1.8 1.4 1.4 1.4 1.3 1.8 2.1 2.0 2.7 1.5 1.6 1.8
Intensity Change (Log2) 0.04 -0.04 -0.21 -0.19 -0.39 -0.38 -0.54 -0.49 0.29 0.27 0.25 0.5 -0.12 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -1.0 -1.0 -3.4 -4.4 -6.5 -7.7 -7.3 2.0 2.1 1.8 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.7 3.7 8.8 7.1 0 0 0 0
Nucleus 0 0 0 0 0 3.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 0.1 0.1 1.7 0.7 4.4 1.7 2.6 0 0 -2.6 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available