Standard name | |
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Human Ortholog | |
Description | 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 4 | 4 | 7 | 12 | 11 | 5 | 13 | 20 | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 6 | 6 | 7 | 9 | 15 | 17 | 14 | 13 | 23 | 26 | 38 | 10 | 9 | 15 | 6 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 189 | 360 | 310 | 194 | 210 | 248 | 246 | 225 | 166 | 155 | 176 | 167 | 337 | 507 | 492 | 220 | 173 | 185 | 179 | 355 | 287 | 227 | 305 | 250 |
Endoplasmic Reticulum | 0 | 11 | 2 | 6 | 13 | 16 | 30 | 51 | 32 | 19 | 24 | 34 | 14 | 16 | 16 | 7 | 5 | 4 | 4 | 3 | 14 | 7 | 15 | 21 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 1 |
Golgi | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 2 | 1 | 3 | 2 | 0 | 1 | 3 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 5 | 2 |
Mitochondria | 2 | 0 | 5 | 3 | 9 | 75 | 82 | 67 | 93 | 99 | 120 | 143 | 0 | 5 | 2 | 11 | 0 | 1 | 0 | 1 | 1 | 3 | 2 | 2 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 2 | 0 | 2 | 0 | 1 | 2 | 0 | 1 | 1 | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 6 | 1 | 4 | 6 | 9 | 1 | 6 | 3 | 5 | 1 | 1 | 0 | 34 | 17 | 12 | 1 | 4 | 6 | 4 | 3 | 7 |
Unique Cell Count | 190 | 367 | 318 | 204 | 235 | 319 | 346 | 341 | 264 | 256 | 299 | 322 | 355 | 526 | 510 | 257 | 185 | 198 | 190 | 373 | 328 | 248 | 345 | 298 |
Labelled Cell Count | 192 | 378 | 327 | 217 | 250 | 365 | 395 | 382 | 321 | 315 | 366 | 414 | 364 | 539 | 530 | 292 | 202 | 207 | 190 | 373 | 328 | 248 | 345 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.4 | 18.9 | 13.2 | 13.0 | 12.2 | 10.6 | 9.5 | 9.9 | 10.5 | 9.9 | 9.7 | 9.9 | 16.9 | 15.9 | 15.4 | 17.3 | 20.2 | 20.3 | 13.4 | 13.5 | 14.6 |
Std Deviation (1e-4) | 2.8 | 3.1 | 2.3 | 2.1 | 2.1 | 1.7 | 1.9 | 1.9 | 1.9 | 2.1 | 1.9 | 2.1 | 2.4 | 2.6 | 2.7 | 4.7 | 4.8 | 5.7 | 2.8 | 2.8 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.12 | -0.31 | -0.48 | -0.42 | -0.34 | -0.41 | -0.44 | -0.42 | 0.36 | 0.26 | 0.22 | 0.39 | 0.61 | 0.62 | 0.02 | 0.02 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 3.8 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.1 | 1.4 | 2.0 | 2.1 | 1.7 | 2.1 | 3.9 | 3.8 | 5.0 | 0.8 | -0.2 | 0.9 | 0.4 | 0 | 0 |
Cytoplasm | -1.5 | -4.0 | -7.6 | -9.2 | -10.3 | -10.8 | -11.2 | -11.7 | -13.2 | -1.7 | -0.9 | -0.8 | -5.3 | -2.2 | -2.3 |
Endoplasmic Reticulum | 0 | 3.5 | 3.3 | 4.8 | 6.8 | 5.9 | 4.3 | 4.6 | 5.5 | 2.8 | 2.4 | 2.4 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.7 | 8.4 | 8.4 | 7.4 | 10.8 | 11.5 | 11.9 | 12.9 | 0 | 0 | 0 | 2.0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 5.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.8789 | 16.2286 | 13.5481 | 13.4053 | 12.4029 | 13.8043 | 11.6397 | 14.8277 | 13.3596 | 13.5498 | 12.7828 | 14.0104 | 14.1391 | 17.528 | 15.5361 | 14.6863 | 14.63 | 15.6955 |
Actin | 0.0103 | 0.0083 | 0.016 | 0.0009 | 0.0014 | 0.017 | 0.0041 | 0.0105 | 0.0195 | 0.002 | 0.0021 | 0.0026 | 0.0122 | 0.0009 | 0.0009 | 0.0061 | 0.0122 | 0.0007 |
Bud | 0.001 | 0.0004 | 0.0014 | 0.0011 | 0.0012 | 0.0005 | 0.0015 | 0.0004 | 0.0009 | 0.0003 | 0.0023 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0002 |
Bud Neck | 0.001 | 0.0005 | 0.004 | 0.0011 | 0.0013 | 0.0019 | 0.0013 | 0.0004 | 0.0008 | 0.0004 | 0.001 | 0.0013 | 0.0006 | 0.0003 | 0.0007 | 0.0021 | 0.0007 | 0.001 |
Bud Periphery | 0.0008 | 0.0004 | 0.0019 | 0.0005 | 0.0013 | 0.0005 | 0.0015 | 0.0001 | 0.0008 | 0.0003 | 0.0039 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Bud Site | 0.0033 | 0.0033 | 0.0102 | 0.0006 | 0.0045 | 0.0003 | 0.0014 | 0.0022 | 0.0022 | 0.0006 | 0.0029 | 0.0001 | 0.0012 | 0.0009 | 0.0013 | 0.0215 | 0.0007 | 0.0001 |
Cell Periphery | 0.001 | 0.0007 | 0.0014 | 0.0002 | 0.0006 | 0.0004 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.7663 | 0.9392 | 0.7856 | 0.8948 | 0.7552 | 0.8368 | 0.7941 | 0.9405 | 0.843 | 0.7846 | 0.7883 | 0.8921 | 0.8135 | 0.9628 | 0.9 | 0.881 | 0.798 | 0.909 |
Cytoplasmic Foci | 0.0132 | 0.0038 | 0.0118 | 0.0126 | 0.0227 | 0.0133 | 0.0187 | 0.0081 | 0.0205 | 0.015 | 0.0156 | 0.015 | 0.0177 | 0.0146 | 0.0112 | 0.01 | 0.0233 | 0.0107 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0614 | 0.0216 | 0.0345 | 0.0032 | 0.0486 | 0.0447 | 0.0465 | 0.0075 | 0.0161 | 0.0494 | 0.013 | 0.029 | 0.0628 | 0.0075 | 0.0213 | 0.0109 | 0.0205 | 0.0324 |
Endosome | 0.0527 | 0.007 | 0.0484 | 0.033 | 0.0533 | 0.043 | 0.0555 | 0.0108 | 0.0475 | 0.083 | 0.0901 | 0.0315 | 0.0406 | 0.0063 | 0.0313 | 0.0363 | 0.0869 | 0.0291 |
Golgi | 0.0113 | 0.002 | 0.0142 | 0.0111 | 0.0057 | 0.0109 | 0.0163 | 0.0017 | 0.0132 | 0.0076 | 0.0085 | 0.0091 | 0.0115 | 0.0014 | 0.0052 | 0.0014 | 0.0079 | 0.0029 |
Lipid Particles | 0.0082 | 0.0008 | 0.0043 | 0 | 0.0045 | 0.0031 | 0.0044 | 0.0002 | 0.005 | 0.0017 | 0.0021 | 0.0031 | 0.0038 | 0.0003 | 0.0009 | 0.0007 | 0.0053 | 0.0002 |
Mitochondria | 0.0235 | 0.0023 | 0.0229 | 0.0132 | 0.0077 | 0.0118 | 0.0196 | 0.0005 | 0.0076 | 0.0294 | 0.0325 | 0.0038 | 0.0032 | 0.0002 | 0.0018 | 0.0005 | 0.0152 | 0.0038 |
None | 0.0022 | 0.0004 | 0.0022 | 0.0006 | 0.0015 | 0.0005 | 0.0015 | 0.0007 | 0.0007 | 0.0005 | 0.0013 | 0.0006 | 0.0048 | 0.0003 | 0.0008 | 0.0006 | 0.006 | 0.0009 |
Nuclear Periphery | 0.0108 | 0.0012 | 0.0108 | 0.0021 | 0.0104 | 0.0017 | 0.0074 | 0.0006 | 0.0018 | 0.002 | 0.0049 | 0.0043 | 0.009 | 0.001 | 0.0023 | 0.0022 | 0.0057 | 0.0017 |
Nucleolus | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0021 | 0 | 0.0001 | 0.0088 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 |
Nucleus | 0.0037 | 0.0012 | 0.003 | 0.0026 | 0.022 | 0.0014 | 0.006 | 0.0028 | 0.0011 | 0.0011 | 0.0042 | 0.0022 | 0.0029 | 0.0008 | 0.0018 | 0.002 | 0.0028 | 0.0017 |
Peroxisomes | 0.004 | 0.0007 | 0.0021 | 0.0001 | 0.0051 | 0.0007 | 0.0007 | 0.0001 | 0.0058 | 0.0006 | 0.0008 | 0.0007 | 0.0022 | 0.0002 | 0.0002 | 0.0009 | 0.0022 | 0.0001 |
Punctate Nuclear | 0.0034 | 0.0001 | 0.0016 | 0.0001 | 0.0019 | 0.0003 | 0.0004 | 0.0002 | 0.0007 | 0.0001 | 0.0005 | 0.0002 | 0.0027 | 0.0001 | 0.0002 | 0.0009 | 0.0015 | 0.0008 |
Vacuole | 0.0171 | 0.0055 | 0.0177 | 0.0186 | 0.0388 | 0.0079 | 0.0149 | 0.003 | 0.009 | 0.017 | 0.018 | 0.0027 | 0.0078 | 0.0017 | 0.0155 | 0.0189 | 0.0063 | 0.0029 |
Vacuole Periphery | 0.0039 | 0.0007 | 0.0051 | 0.0035 | 0.0101 | 0.0031 | 0.0029 | 0.0005 | 0.0033 | 0.004 | 0.0071 | 0.0011 | 0.0015 | 0.0003 | 0.0033 | 0.0029 | 0.0033 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 85.105 | 96.5868 | 93.2712 | 80.1161 | 84.9156 | 68.0463 | 70.7358 | 74.0394 | 68.1052 | 80.3019 |
Translational Efficiency | 1.5423 | 1.3081 | 1.2441 | 1.1858 | 1.2097 | 1.5894 | 1.3756 | 1.1083 | 1.1183 | 1.1258 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2273 | 1428 | 1376 | 1436 | 1885 | 1734 | 1113 | 142 | 4158 | 3162 | 2489 | 1578 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1287.38 | 1276.80 | 1612.41 | 1719.15 | 1223.36 | 1356.30 | 1763.87 | 1920.42 | 1258.36 | 1320.40 | 1680.14 | 1737.26 |
Standard Deviation | 188.30 | 236.73 | 307.34 | 280.22 | 181.49 | 221.21 | 256.47 | 308.33 | 187.97 | 231.75 | 295.47 | 288.67 |
Intensity Change Log 2 | — | -0.011905 | 0.324781 | 0.417257 | — | 0.148827 | 0.527895 | 0.650573 | — | 0.068650 | 0.427322 | 0.535655 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000144 | 0.000434 | 0.000424 | 0.000546 | 0.000130 | 0.000366 | 0.000291 | 0.002075 | 0.000138 | 0.000397 | 0.000365 | 0.000683 |
Bud Neck | 0.000231 | 0.000647 | 0.001542 | 0.001371 | 0.000247 | 0.000388 | 0.001447 | 0.005046 | 0.000238 | 0.000505 | 0.001499 | 0.001702 |
Bud Site | 0.019700 | 0.036992 | 0.020807 | 0.028987 | 0.019554 | 0.025853 | 0.015521 | 0.048518 | 0.019634 | 0.030883 | 0.018443 | 0.030745 |
Cell Periphery | 0.000743 | 0.000639 | 0.001376 | 0.003231 | 0.000496 | 0.000285 | 0.000786 | 0.012081 | 0.000631 | 0.000445 | 0.001112 | 0.004028 |
Cytoplasm | 0.754355* | 0.774412* | 0.799222* | 0.851635* | 0.795390* | 0.789788* | 0.802795* | 0.710586* | 0.772958* | 0.782844* | 0.800820* | 0.838942* |
Cytoplasmic Foci | 0.127030 | 0.088940 | 0.035809 | 0.018027 | 0.097589 | 0.103589 | 0.026907 | 0.028206 | 0.113683 | 0.096974 | 0.031828 | 0.018943 |
Eisosomes | 0.000004 | 0.000005 | 0.000003 | 0.000004 | 0.000004 | 0.000005 | 0.000002 | 0.000012 | 0.000004 | 0.000005 | 0.000003 | 0.000005 |
Endoplasmic Reticulum | 0.005090 | 0.004050 | 0.002156 | 0.000516 | 0.004280 | 0.002368 | 0.002002 | 0.001032 | 0.004723 | 0.003128 | 0.002087 | 0.000563 |
Endosome | 0.005214 | 0.006547 | 0.007637 | 0.002638 | 0.004085 | 0.006040 | 0.006502 | 0.008319 | 0.004702 | 0.006269 | 0.007130 | 0.003149 |
Golgi | 0.023512 | 0.023795 | 0.033895 | 0.012386 | 0.019033 | 0.026760 | 0.011639 | 0.016464 | 0.021481 | 0.025421 | 0.023943 | 0.012753 |
Lipid Particles | 0.000808 | 0.001526 | 0.004845 | 0.009225 | 0.000760 | 0.000860 | 0.005148 | 0.030469 | 0.000786 | 0.001161 | 0.004980 | 0.011137 |
Mitochondria | 0.001574 | 0.001492 | 0.000084 | 0.000806 | 0.001311 | 0.001455 | 0.000076 | 0.000042 | 0.001455 | 0.001471 | 0.000081 | 0.000737 |
Mitotic Spindle | 0.000795 | 0.002275 | 0.004373 | 0.001614 | 0.001407 | 0.003906 | 0.005759 | 0.001476 | 0.001072 | 0.003169 | 0.004993 | 0.001601 |
None | 0.000368 | 0.000218 | 0.000540 | 0.000159 | 0.000568 | 0.000172 | 0.000161 | 0.000218 | 0.000459 | 0.000193 | 0.000370 | 0.000164 |
Nuclear Periphery | 0.000015 | 0.000049 | 0.000097 | 0.000026 | 0.000025 | 0.000015 | 0.000111 | 0.000066 | 0.000020 | 0.000030 | 0.000103 | 0.000030 |
Nuclear Periphery Foci | 0.000312 | 0.001412 | 0.006014 | 0.001913 | 0.000691 | 0.000345 | 0.004109 | 0.004987 | 0.000484 | 0.000827 | 0.005162 | 0.002190 |
Nucleolus | 0.000108 | 0.000439 | 0.000051 | 0.000091 | 0.000038 | 0.000049 | 0.000075 | 0.000096 | 0.000076 | 0.000225 | 0.000062 | 0.000092 |
Nucleus | 0.001046 | 0.003235 | 0.004246 | 0.002736 | 0.001243 | 0.001116 | 0.003693 | 0.003775 | 0.001135 | 0.002073 | 0.003999 | 0.002829 |
Peroxisomes | 0.000224 | 0.000491 | 0.000146 | 0.000610 | 0.000202 | 0.000418 | 0.000211 | 0.001075 | 0.000214 | 0.000451 | 0.000175 | 0.000652 |
Vacuole | 0.021044 | 0.025821 | 0.055738 | 0.057733 | 0.019159 | 0.017362 | 0.101185 | 0.118014 | 0.020190 | 0.021182 | 0.076060 | 0.063158 |
Vacuole Periphery | 0.037682 | 0.026581 | 0.020995 | 0.005745 | 0.033791 | 0.018860 | 0.011580 | 0.007442 | 0.035918 | 0.022347 | 0.016785 | 0.005898 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.38 | -6.92 | -6.64 | -1.75 | -1.79 | -2.44 | -4.94 | -2.69 | -2.41 | -2.49 | -4.46 | -8.49 | -6.18 | -3.11 | -3.59 |
Bud Neck | -2.87 | -10.44 | -12.45 | -4.91 | 1.04 | -3.22 | -11.12 | -7.06 | -6.86 | -5.05 | -3.83 | -15.03 | -13.80 | -10.02 | -1.18 |
Bud Site | -8.11 | -0.95 | -4.65 | 2.89 | -3.56 | -3.98 | 2.28 | -5.05 | -3.91 | -5.58 | -8.65 | 0.57 | -6.07 | -0.07 | -6.10 |
Cell Periphery | 0.35 | -1.91 | -4.52 | -5.64 | -3.83 | 3.96 | -2.42 | -4.86 | -4.95 | -4.74 | 1.12 | -2.50 | -6.76 | -7.58 | -6.04 |
Cytoplasm | -2.33 | -4.74 | -12.08 | -8.59 | -5.80 | 0.70 | 0.12 | 3.94 | 3.68 | 3.83 | -1.67 | -3.56 | -9.20 | -7.33 | -5.25 |
Cytoplasmic Foci | 8.16 | 24.09 | 29.63 | 20.51 | 8.60 | -1.31 | 20.33 | 12.33 | 13.12 | -0.13 | 5.04 | 31.31 | 35.32 | 29.36 | 7.85 |
Eisosomes | -1.53 | 3.58 | 0.16 | 1.63 | -3.40 | -1.63 | 3.19 | -3.08 | -2.81 | -3.45 | -2.09 | 4.77 | -2.26 | -0.22 | -5.33 |
Endoplasmic Reticulum | 1.09 | 3.88 | 6.40 | 5.56 | 5.70 | 2.38 | 2.70 | 4.99 | 2.23 | 1.23 | 2.55 | 4.67 | 8.70 | 6.11 | 4.28 |
Endosome | -2.71 | -4.53 | 6.77 | 7.40 | 8.64 | -4.46 | -4.02 | -1.81 | -0.93 | -0.68 | -4.76 | -6.07 | 4.21 | 7.06 | 8.00 |
Golgi | -0.19 | -4.88 | 7.94 | 7.05 | 9.56 | -4.71 | 6.54 | 1.27 | 3.80 | -1.69 | -3.59 | -1.55 | 7.25 | 8.86 | 6.90 |
Lipid Particles | -3.70 | -9.29 | -10.34 | -9.35 | -4.92 | -0.60 | -7.83 | -6.57 | -6.55 | -5.54 | -2.95 | -12.07 | -12.08 | -11.60 | -6.72 |
Mitochondria | 0.14 | 7.25 | 1.55 | 0.96 | -1.43 | -0.41 | 6.97 | 7.17 | 4.58 | 1.51 | -0.05 | 9.95 | 1.65 | 1.44 | -1.43 |
Mitotic Spindle | -1.87 | -8.28 | -1.73 | 0.18 | 2.84 | -2.37 | -3.30 | -0.02 | 2.20 | 3.15 | -3.18 | -6.10 | -1.32 | 1.29 | 3.44 |
None | 2.26 | -0.96 | 3.09 | 2.18 | 2.33 | 1.38 | 1.41 | 1.20 | -0.72 | -0.91 | 1.96 | 0.59 | 2.14 | 1.15 | 2.22 |
Nuclear Periphery | -2.28 | -12.59 | -2.99 | 1.24 | 9.13 | 1.89 | -4.22 | -3.70 | -4.90 | 2.05 | -1.50 | -8.55 | -2.88 | -0.39 | 6.86 |
Nuclear Periphery Foci | -4.12 | -13.31 | -9.61 | -1.88 | 8.80 | 1.57 | -5.87 | -5.76 | -6.47 | -0.96 | -2.15 | -13.23 | -9.05 | -6.93 | 7.71 |
Nucleolus | -1.33 | 0.78 | 0.19 | 1.44 | -7.01 | -0.60 | -7.69 | -4.91 | -2.19 | -1.34 | -1.30 | 0.30 | -0.46 | 1.21 | -5.52 |
Nucleus | -2.93 | -10.34 | -9.45 | 0.16 | 3.48 | 0.91 | -12.19 | -4.68 | -4.83 | 0.46 | -2.75 | -15.12 | -10.04 | -2.80 | 4.04 |
Peroxisomes | -3.10 | 2.70 | -2.96 | -0.89 | -3.50 | -4.13 | -1.13 | -4.18 | -3.10 | -4.03 | -4.87 | 1.46 | -3.64 | -1.68 | -3.86 |
Vacuole | -2.36 | -11.01 | -12.89 | -10.72 | -0.80 | 1.19 | -17.51 | -7.26 | -7.39 | -1.13 | -0.77 | -20.24 | -15.23 | -14.78 | 3.21 |
Vacuole Periphery | 4.27 | 7.12 | 17.86 | 10.56 | 8.82 | 7.24 | 12.21 | 10.07 | 4.88 | 1.76 | 8.27 | 12.51 | 23.47 | 13.89 | 9.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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