Standard name
Human Ortholog
Description S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.92 1.0 0.97 0.93 0.88 0.71 0.75 0.71 0.63 0.7 0.67 0.97 0.94 0.96 0.96 0.98 0.96 0.97 0.84 0.98 0.9 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.15 0.22 0.27 0.24 0.26 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.14 0.1 0.08 0.2 0.06 0.08 0 0 0 0 0 0 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0
Bud 0 1 0 0 0 1 3 1 1 2 2 4 1 0 0 0 1 0 0 0 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 0 3 2 7 1 4 8 8 7 13 0 2 4 5 5 5 0 0 0 0 0 0
Cytoplasm 246 207 200 207 258 243 290 199 168 239 199 245 270 270 327 326 297 299 244 192 195 223 257 295
Endoplasmic Reticulum 1 3 1 2 0 4 13 1 3 3 12 7 5 9 8 15 10 11 0 0 0 6 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0
Mitochondria 1 1 1 4 9 26 63 58 65 89 74 114 1 0 0 0 0 1 1 1 0 1 0 0
Nucleus 2 17 1 1 1 4 9 7 7 12 12 7 2 4 2 4 1 3 0 12 0 2 3 5
Nuclear Periphery 0 0 0 0 1 1 6 0 0 3 2 2 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 5 0 2 17 9 56 28 19 74 16 31 7 10 5 6 3 11 0 19 0 5 8 7
Unique Cell Count 252 225 201 213 278 276 410 267 237 378 286 367 278 288 340 340 302 310 251 228 200 249 281 322
Labelled Cell Count 255 242 204 219 289 297 441 298 271 430 325 425 286 296 348 359 317 330 251 228 200 249 281 322


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.5 20.7 12.5 11.7 9.5 8.4 7.5 7.7 7.2 7.0 7.2 7.1 14.1 14.2 14.3 20.7 19.6 20.4 18.6 18.1 18.3
Std Deviation (1e-4) 3.2 4.3 1.9 2.0 1.6 1.6 1.5 1.5 1.3 1.6 1.4 1.5 2.3 2.2 1.9 5.0 5.0 5.1 3.2 3.2 3.5
Intensity Change (Log2) – – – -0.09 -0.39 -0.58 -0.73 -0.7 -0.8 -0.84 -0.79 -0.82 0.17 0.18 0.19 0.73 0.65 0.71 0.57 0.53 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.8 -3.5 -4.8 -8.4 -7.5 -8.1 -9.7 -8.4 -9.1 -1.9 -3.2 -2.4 -2.5 -1.2 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 2.5 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 5.6 6.8 7.9 7.3 7.6 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.6 0 5.5 4.7 4.1 6.7 3.4 4.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0103 0.0016 0.0041 0.004 0.0035 0.0007 0.0137 0.0002 0.0014 0.0111 0.0122 0.0003 – – – – – –
Bud 0.0003 0.0027 0.0014 0.0007 0.007 0.0004 0.0005 0.0001 0.0001 0.0005 0.0002 0.0001 – – – – – –
Bud Neck 0.0011 0.0004 0.0005 0.001 0.0014 0.0012 0.0012 0.0002 0.0003 0.0004 0.0009 0.0009 – – – – – –
Bud Periphery 0.0003 0.0011 0.0006 0.0005 0.0027 0.0002 0.0004 0 0 0.0002 0.0001 0 – – – – – –
Bud Site 0.0013 0.0009 0.0008 0.0003 0.0005 0.0001 0.0011 0.0001 0.0001 0.0005 0.0004 0 – – – – – –
Cell Periphery 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 – – – – – –
Cytoplasm 0.842 0.9447 0.8987 0.9252 0.8641 0.8931 0.8879 0.9791 0.963 0.8993 0.9021 0.9546 – – – – – –
Cytoplasmic Foci 0.0139 0.002 0.0084 0.0031 0.0126 0.0029 0.0089 0.0009 0.002 0.0085 0.0061 0.002 – – – – – –
Eisosomes 0.0001 0 0.0001 0 0 0 0 0 0 0.0001 0 0 – – – – – –
Endoplasmic Reticulum 0.0077 0.0019 0.0029 0.0018 0.007 0.0028 0.0084 0.0006 0.0023 0.0017 0.0093 0.0019 – – – – – –
Endosome 0.0181 0.0013 0.0095 0.0023 0.0205 0.002 0.0123 0.0004 0.0021 0.0079 0.0079 0.0012 – – – – – –
Golgi 0.0025 0.0001 0.0015 0.0005 0.001 0.0001 0.0039 0 0.0004 0.0062 0.0049 0.0001 – – – – – –
Lipid Particles 0.0036 0.0001 0.0015 0.0001 0.0005 0.0005 0.0006 0 0.0002 0.0105 0.0009 0 – – – – – –
Mitochondria 0.0017 0.002 0.0042 0.0005 0.0012 0.0003 0.002 0.0001 0.0007 0.0066 0.0004 0.0002 – – – – – –
None 0.0104 0.002 0.003 0.0011 0.0051 0.0016 0.0031 0.0006 0.0014 0.0027 0.0012 0.0012 – – – – – –
Nuclear Periphery 0.0167 0.0035 0.0062 0.0028 0.041 0.0057 0.0117 0.0013 0.0022 0.002 0.0051 0.0027 – – – – – –
Nucleolus 0.0004 0.0001 0.0004 0 0.0003 0 0.0001 0 0 0.0001 0 0 – – – – – –
Nucleus 0.0592 0.0329 0.0507 0.0547 0.0212 0.0819 0.0392 0.016 0.0225 0.0296 0.0459 0.0337 – – – – – –
Peroxisomes 0.0017 0.0002 0.0009 0.0003 0.0004 0.0041 0.0017 0 0.0002 0.0101 0.0003 0 – – – – – –
Punctate Nuclear 0.0056 0.0014 0.0028 0.0006 0.0056 0.0013 0.0013 0.0001 0.0005 0.0012 0.0014 0.0005 – – – – – –
Vacuole 0.0021 0.0009 0.0011 0.0006 0.003 0.0007 0.0013 0.0002 0.0003 0.0006 0.0005 0.0004 – – – – – –
Vacuole Periphery 0.0009 0.0002 0.0005 0.0002 0.0013 0.0002 0.0009 0 0.0001 0.0003 0.0002 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.4039 45.5505 22.1239 31.0045 24.6243 33.3338 33.286 33.6281 27.1417 28.42
Translational Efficiency 1.1617 0.9968 1.5383 1.2314 1.435 0.8239 1.0344 0.9634 1.0954 0.9729

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2212 961 270 1515 1214 749 1308 1667 3426 1710 1578 3182

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1372.93 1336.97 1764.78 1774.39 1208.52 1302.46 1681.61 1819.00 1314.67 1321.85 1695.84 1797.76
Standard Deviation 214.28 302.94 239.80 286.51 251.29 256.54 249.16 259.09 241.26 284.07 249.56 273.40
Intensity Change Log 2 — -0.038291 0.362230 0.370065 — 0.107998 0.476602 0.589904 — 0.032045 0.416904 0.477164

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000407 0.001088 0.007995 0.000220 0.000391 0.000306 0.000487 0.000121 0.000400 0.000440 0.004061
Bud Neck 0.000293 0.000840 0.004957 0.004279 0.000541 0.000659 0.001999 0.001473 0.000381 0.000761 0.002505 0.002809
Bud Site 0.001184 0.004911 0.007011 0.015045 0.003704 0.007039 0.003391 0.009872 0.002077 0.005843 0.004011 0.012335
Cell Periphery 0.000028 0.000118 0.000330 0.000122 0.000074 0.000105 0.000078 0.000070 0.000044 0.000112 0.000121 0.000095
Cytoplasm 0.855559* 0.808890* 0.457750* 0.587507* 0.844470* 0.827933* 0.660864* 0.706591* 0.851630* 0.817231* 0.626111* 0.649893*
Cytoplasmic Foci 0.006792 0.014052 0.011173 0.010899 0.020155 0.023496 0.006171 0.005191 0.011527 0.018189 0.007027 0.007909
Eisosomes 0.000001 0.000005 0.000016 0.000009 0.000004 0.000008 0.000005 0.000004 0.000002 0.000006 0.000007 0.000006
Endoplasmic Reticulum 0.000192 0.000783 0.002147 0.000541 0.000876 0.000613 0.000488 0.000216 0.000435 0.000708 0.000772 0.000371
Endosome 0.000126 0.001603 0.003415 0.011029 0.000874 0.002104 0.003585 0.002117 0.000391 0.001823 0.003556 0.006360
Golgi 0.000211 0.002181 0.001057 0.010561 0.001211 0.001958 0.000716 0.002297 0.000565 0.002083 0.000775 0.006232
Lipid Particles 0.000140 0.000370 0.002176 0.000996 0.000418 0.001108 0.000818 0.000485 0.000238 0.000693 0.001050 0.000728
Mitochondria 0.000147 0.000508 0.000474 0.006047 0.000869 0.001307 0.000723 0.001349 0.000403 0.000858 0.000680 0.003586
Mitotic Spindle 0.001818 0.005694 0.016706 0.119343* 0.001854 0.005078 0.019508 0.025955 0.001831 0.005424 0.019028 0.070419
None 0.012448 0.007536 0.010415 0.008278 0.004934 0.006307 0.009465 0.011195 0.009785 0.006997 0.009627 0.009806
Nuclear Periphery 0.000059 0.000152 0.001454 0.000862 0.000123 0.000134 0.000760 0.000271 0.000081 0.000144 0.000878 0.000552
Nuclear Periphery Foci 0.000668 0.002829 0.004470 0.004197 0.003247 0.003300 0.002541 0.001107 0.001582 0.003035 0.002871 0.002578
Nucleolus 0.000073 0.000326 0.000351 0.000421 0.000161 0.001053 0.001384 0.000111 0.000104 0.000645 0.001208 0.000259
Nucleus 0.116136 0.134423* 0.338628* 0.172781* 0.098763 0.092925 0.241012* 0.212106* 0.109980 0.116246 0.257715* 0.193383*
Peroxisomes 0.000049 0.000199 0.000201 0.003052 0.000085 0.000373 0.000388 0.000281 0.000062 0.000275 0.000356 0.001600
Vacuole 0.003710 0.011987 0.135627 0.031882 0.016820 0.020869 0.045392 0.017188 0.008356 0.015877 0.060832 0.024184
Vacuole Periphery 0.000299 0.002186 0.000552 0.004155 0.000598 0.003240 0.000405 0.001635 0.000405 0.002647 0.000430 0.002835

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.12 -10.54 -2.03 -1.55 -0.51 -2.36 -1.66 -1.81 0.90 -0.23 -6.35 -7.88 -2.24 -1.42 -1.29
Bud Neck -7.00 -14.20 -11.18 -7.77 7.73 -1.54 -8.49 -8.11 -5.60 4.84 -6.92 -14.01 -13.94 -9.15 4.83
Bud Site -5.16 -12.46 -8.17 -1.41 2.58 -2.01 0.62 -1.59 1.11 -2.06 -4.76 -6.93 -7.22 0.17 -2.51
Cell Periphery -7.94 -12.60 -15.10 -1.33 9.27 -2.54 -4.74 -3.51 0.80 2.52 -8.21 -13.68 -15.97 -0.23 6.02
Cytoplasm 9.27 37.62 43.52 26.36 -15.20 2.41 38.97 27.06 18.33 -15.61 8.66 54.14 51.32 31.09 -15.85
Cytoplasmic Foci -7.07 -4.55 -1.77 6.13 3.68 -1.82 10.57 9.01 7.85 -3.63 -6.26 6.97 5.88 9.95 -2.02
Eisosomes -4.64 -15.67 -13.07 -1.91 10.96 -2.60 -7.96 -1.88 2.06 7.55 -5.22 -18.43 -12.17 0.58 10.00
Endoplasmic Reticulum -5.25 -9.49 -1.86 4.48 8.97 1.37 2.22 4.60 5.49 10.48 -3.06 -4.49 3.82 6.81 12.05
Endosome -3.34 -8.34 -4.07 -0.78 1.52 -2.05 -0.90 1.40 2.60 1.69 -4.06 -3.86 -3.49 1.12 0.69
Golgi -2.36 -6.39 -3.09 0.31 -1.80 -0.82 2.20 1.37 1.88 -0.89 -2.58 1.11 -1.78 1.42 -2.59
Lipid Particles -3.82 -10.95 -10.18 -8.68 4.30 -1.36 -6.20 -4.96 -0.11 0.92 -2.38 -13.53 -11.71 -3.70 0.51
Mitochondria -1.82 -10.11 -2.28 -1.40 -1.15 -0.35 0.37 0.57 1.29 0.88 -1.04 -0.71 -1.07 0.05 -0.75
Mitotic Spindle -2.68 -3.57 -9.61 -7.11 -4.89 -1.95 -8.42 -5.76 -3.32 3.52 -3.30 -9.04 -11.14 -7.54 -0.47
None 5.63 4.25 5.47 -1.72 -1.43 -0.67 -5.32 -5.32 -2.63 0.26 3.66 0.97 1.34 -3.27 0.30
Nuclear Periphery -3.85 -15.79 -17.68 -13.26 8.73 -0.44 -24.31 -11.96 -11.32 17.71 -3.88 -30.16 -21.99 -16.23 15.46
Nuclear Periphery Foci -7.37 -8.90 -6.83 1.63 4.78 -0.54 4.89 7.06 4.99 2.72 -6.40 -5.39 -2.32 4.70 2.76
Nucleolus -1.30 -12.63 -6.82 0.50 1.80 -1.57 -1.49 0.56 1.68 1.75 -2.08 -2.09 -3.29 1.70 1.57
Nucleus -4.04 -16.96 -18.63 -11.29 9.71 0.98 -29.12 -22.54 -21.52 9.69 -2.18 -34.27 -29.16 -21.87 13.73
Peroxisomes -1.70 -6.28 -1.61 -0.88 -1.05 -2.72 -0.80 -1.26 2.28 -0.02 -2.92 -2.03 -1.77 -0.16 -1.22
Vacuole -8.88 -15.87 -28.24 -23.57 5.84 -2.26 -19.97 -16.37 -12.12 8.05 -8.08 -27.26 -33.49 -26.03 6.03
Vacuole Periphery -1.70 -0.85 -2.08 1.27 -1.82 -1.64 2.64 1.12 1.82 -1.11 -2.39 2.47 -0.82 2.23 -2.58
Standard name
Human Ortholog
Description S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Spe2

Spe2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available