Standard name
Human Ortholog
Description Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 – 0 0.08 0.05 0.05 0.05 0.1 0.1 0.17 0.13 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.79 0.85 0.86 0.66 0.55 0.54 – 0.53 0.52 0.61 0.45 0.75 0.71 0.53 0.52 0.45 0.39 0.84 0.84 0.72 0.71 0.61 0.45
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0.05 0 0.14 0 0.05 – 0.08 0.21 0.15 0.21 0.07 0 0.16 0.05 0.06 0.07 0.06 0.05 0.13 0.07 0.14 0.24
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.19 0.08 0.08 0.18 0.38 0.34 – 0.32 0.23 0.18 0.25 0.09 0.15 0.22 0.24 0.33 0.39 0.07 0.09 0.08 0.1 0.16 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 1 0 4 3 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 3 5 3 3 6 – 3 9 5 6 4 7 9 32 22 31 1 0 2 1 3 2
Endoplasmic Reticulum 1 1 0 0 0 0 0 – 0 0 0 0 2 0 0 6 4 9 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 3 – 1 1 0 1 0 1 1 1 2 2 0 0 0 0 2 3
Golgi 0 2 1 0 0 6 3 – 0 3 2 0 2 0 0 4 15 7 0 0 1 0 0 1
Mitochondria 0 0 0 0 1 0 0 – 4 3 4 7 0 0 0 2 7 1 0 0 0 2 0 0
Nucleus 295 200 128 87 48 70 76 – 47 55 67 50 60 51 47 98 75 73 303 268 128 54 86 45
Nuclear Periphery 0 0 0 1 0 0 1 – 0 0 0 0 1 0 0 0 0 0 1 0 2 1 0 1
Nucleolus 16 8 8 4 10 4 7 – 7 22 17 23 6 3 14 10 10 14 22 16 22 5 19 23
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 1
Vac/Vac Membrane 22 49 12 8 13 48 48 – 28 24 20 28 7 11 19 45 55 72 25 27 13 7 23 16
Unique Cell Count 333 253 151 101 73 127 141 88 106 110 110 80 72 88 187 167 187 362 320 177 77 143 100
Labelled Cell Count 340 262 152 105 75 131 145 90 117 116 117 82 74 90 199 193 210 362 320 177 77 143 100


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.4 32.1 27.5 25.8 15.2 24.9 22.4 – 19.3 19.0 18.3 16.5 29.5 30.8 27.7 25.3 31.5 27.8 26.8 31.6 26.5
Std Deviation (1e-4) 8.2 8.1 6.5 6.0 5.1 5.9 5.5 – 5.4 5.6 4.9 5.1 9.5 9.6 9.7 10.3 11.7 12.5 9.9 11.1 10.5
Intensity Change (Log2) – – – -0.09 -0.86 -0.15 -0.3 – -0.51 -0.54 -0.59 -0.74 0.1 0.16 0.01 -0.12 0.19 0.01 -0.04 0.2 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 4.5 3.7 4.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 3.4 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0.3 -3.3 -5.4 -5.7 – -5.3 -5.7 -4.4 -6.7 -1.8 -2.4 -5.3 -6.3 -7.4 -8.5
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.2 -0.9 -0.1 – 0.8 3.8 2.8 3.8 0 0 2.7 0 0.3 0.8
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.2 6.0 5.5 – 4.8 3.3 2.5 3.9 0.2 1.7 3.0 3.9 5.5 6.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.6141 27.4412 23.7735 23.149 18.413 22.6824 29.001 36.8251 31.1057 30.8683 33.9522 31.8946 23.844 31.3153 25.2651 28.7513 26.1396 26.6356
Actin 0.0013 0 0.0002 0.0001 0.0046 0.0007 0.02 0.0001 0.0003 0.0002 0.0001 0.0001 0.0092 0 0.0066 0 0.0003 0.0005
Bud 0.0007 0 0.0001 0.0001 0.0004 0.0004 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0004 0 0.0001 0 0.0003 0.0002
Bud Neck 0.0005 0.0001 0.0002 0.0001 0.0007 0.0008 0.0003 0.0001 0.0001 0.0001 0.0002 0.0007 0.0002 0 0.0001 0 0.0005 0.0006
Bud Periphery 0.0009 0 0.0002 0 0.0009 0.0008 0.0004 0 0.0001 0 0 0.0002 0.0005 0 0.0001 0 0.0004 0.0002
Bud Site 0.001 0 0.0001 0.0001 0.0009 0.0002 0.0007 0.0002 0.0002 0.0001 0 0 0.001 0 0.0004 0 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0.0005 0.0003 0.0007 0.0004 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0004 0.0001
Cytoplasm 0.0303 0.0067 0.0104 0.0183 0.0034 0.0015 0.001 0.0016 0.0014 0.0005 0.0439 0.0003 0.0054 0.0002 0.0078 0.0002 0.0174 0.0038
Cytoplasmic Foci 0.012 0 0.0005 0.0288 0.0016 0.0004 0.0033 0.0033 0.008 0 0.0005 0 0.0147 0 0.005 0 0.002 0.0014
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0026 0 0.0003 0.0005 0.0062 0.0017 0.0028 0 0.0002 0 0.0001 0.0001 0.005 0 0.0019 0 0.001 0.0011
Endosome 0.0065 0 0.006 0.0017 0.0103 0.011 0.0122 0.0001 0.0003 0.0001 0.0008 0.0002 0.0249 0 0.0082 0 0.0011 0.009
Golgi 0.001 0 0.001 0.0003 0.0013 0.0007 0.0031 0 0.0001 0 0 0 0.0042 0 0.005 0 0.0001 0.0005
Lipid Particles 0.0042 0 0.0002 0.006 0.0015 0.0009 0.0034 0 0.0017 0 0 0 0.0079 0 0.0055 0 0.0004 0.0003
Mitochondria 0.0048 0.0006 0.0013 0.001 0.0069 0.004 0.0032 0.0002 0.0094 0.0023 0.0018 0.0006 0.0035 0.0003 0.0071 0.0003 0.0019 0.0014
None 0.0024 0 0.0001 0.003 0.0021 0.0002 0.0018 0.0001 0.0011 0 0.0001 0 0.0044 0 0.0034 0 0.0004 0.0005
Nuclear Periphery 0.0414 0.0098 0.0105 0.0144 0.063 0.024 0.017 0.0026 0.0132 0.0106 0.016 0.0143 0.0216 0.0086 0.0119 0.0029 0.0091 0.0116
Nucleolus 0.0423 0.0068 0.0111 0.0076 0.0878 0.0339 0.0223 0.0059 0.0269 0.0127 0.0278 0.0345 0.022 0.0071 0.0078 0.0023 0.0502 0.0119
Nucleus 0.8229 0.9751 0.9516 0.9058 0.7764 0.9113 0.8944 0.9492 0.9291 0.9716 0.8988 0.945 0.8251 0.9834 0.9182 0.9942 0.9027 0.9486
Peroxisomes 0.0005 0 0.0001 0.0003 0.0002 0.0001 0.0013 0.0001 0.0004 0 0 0 0.0012 0 0.0017 0 0 0.0002
Punctate Nuclear 0.0141 0.0001 0.0001 0.0071 0.0139 0.0008 0.009 0.0364 0.004 0.0004 0.0003 0.0005 0.0415 0 0.0064 0 0.0046 0.0055
Vacuole 0.0053 0.0002 0.0039 0.0024 0.008 0.0028 0.0014 0 0.0011 0.0002 0.0012 0.0013 0.0038 0 0.0008 0 0.0031 0.0013
Vacuole Periphery 0.0049 0.0004 0.0017 0.0019 0.0092 0.0033 0.0017 0 0.002 0.0009 0.0082 0.0019 0.0032 0.0002 0.0019 0.0001 0.0035 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 134.1394 131.5168 147.3435 137.423 134.6458 167.221 146.0134 132.8655 166.9565 109.9634
Translational Efficiency 1.6032 1.6218 1.4314 1.9577 1.2981 1.3361 1.5221 1.6414 1.5302 1.5004

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
599 688 1746 2039 1923 1741 1829 1807 2522 2429 3575 3846

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1671.46 2047.19 2543.54 2887.70 2030.62 2285.45 2526.75 2796.76 1945.32 2217.96 2534.95 2844.97
Standard Deviation 383.61 587.33 527.11 594.62 430.99 475.37 654.16 617.71 447.15 520.77 595.56 607.28
Intensity Change Log 2 — 0.292536 0.605729 0.788812 — 0.170558 0.315363 0.461836 — 0.226910 0.453732 0.618687

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001109 0.000918 0.000857 0.000858 0.000810 0.000767 0.000880 0.001026 0.000881 0.000810 0.000869 0.000937
Bud Neck 0.016919 0.013748 0.022239 0.027103 0.013697 0.014000 0.022754 0.027458 0.014462 0.013929 0.022502 0.027270
Bud Site 0.001389 0.001450 0.001104 0.001675 0.000365 0.000899 0.001283 0.001740 0.000609 0.001056 0.001196 0.001705
Cell Periphery 0.000191 0.000213 0.000097 0.000165 0.000066 0.000263 0.000133 0.000179 0.000096 0.000249 0.000116 0.000172
Cytoplasm 0.003273 0.006639 0.001503 0.001615 0.000379 0.000724 0.003753 0.002153 0.001066 0.002399 0.002654 0.001868
Cytoplasmic Foci 0.000251 0.000366 0.000235 0.000881 0.000040 0.000411 0.000712 0.000259 0.000090 0.000398 0.000479 0.000589
Eisosomes 0.000035 0.000031 0.000034 0.000025 0.000024 0.000031 0.000032 0.000030 0.000027 0.000031 0.000033 0.000027
Endoplasmic Reticulum 0.002683 0.000845 0.000761 0.001088 0.001952 0.001738 0.000759 0.001633 0.002125 0.001485 0.000760 0.001344
Endosome 0.000189 0.000184 0.000051 0.000398 0.000061 0.000398 0.000495 0.000313 0.000091 0.000337 0.000278 0.000358
Golgi 0.000079 0.000171 0.000105 0.000527 0.000036 0.000215 0.000376 0.000362 0.000046 0.000203 0.000244 0.000449
Lipid Particles 0.000036 0.000055 0.000014 0.000216 0.000010 0.000117 0.000056 0.000050 0.000016 0.000099 0.000036 0.000138
Mitochondria 0.002586 0.002708 0.003305 0.004197 0.002091 0.001949 0.002942 0.004212 0.002209 0.002164 0.003119 0.004204
Mitotic Spindle 0.000094 0.003287 0.001293 0.007111 0.000006 0.000386 0.003786 0.000974 0.000027 0.001208 0.002569 0.004228
None 0.002218 0.011131 0.001792 0.001353 0.000709 0.001254 0.003063 0.001232 0.001067 0.004052 0.002442 0.001296
Nuclear Periphery 0.001129 0.000700 0.001297 0.002842 0.001141 0.000909 0.000787 0.002039 0.001138 0.000850 0.001036 0.002465
Nuclear Periphery Foci 0.000195 0.000904 0.000061 0.000613 0.000084 0.000437 0.000245 0.000351 0.000111 0.000569 0.000155 0.000490
Nucleolus 0.023729 0.030390 0.028457 0.021022 0.030639 0.030556 0.021015 0.018601 0.028998 0.030509 0.024649 0.019885
Nucleus 0.942138* 0.923891* 0.935043* 0.925437* 0.946747* 0.942574* 0.935487* 0.934689* 0.945652* 0.937282* 0.935270* 0.929784*
Peroxisomes 0.000594 0.000598 0.000865 0.000487 0.000269 0.000383 0.000647 0.000299 0.000346 0.000444 0.000753 0.000399
Vacuole 0.000864 0.001046 0.000461 0.001368 0.000486 0.001040 0.000551 0.000974 0.000576 0.001042 0.000507 0.001183
Vacuole Periphery 0.000298 0.000725 0.000427 0.001018 0.000387 0.000946 0.000245 0.001427 0.000366 0.000883 0.000334 0.001210

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.38 2.57 3.83 0.25 1.83 1.05 -6.05 -6.26 -6.80 -2.54 1.84 -4.52 -4.49 -5.80 -1.01
Bud Neck 2.00 -5.67 -8.67 -11.19 -4.04 -0.49 -11.93 -12.73 -11.71 -3.53 0.55 -14.56 -17.65 -16.86 -5.29
Bud Site 0.51 0.77 0.18 -0.48 -1.22 -2.41 -13.68 -5.33 -3.41 -1.74 -1.35 -6.06 -5.49 -3.33 -2.10
Cell Periphery -0.50 2.67 0.97 1.37 -2.11 -1.92 -7.06 -4.07 0.75 -1.31 -2.06 -3.28 -3.98 1.03 -2.29
Cytoplasm -1.49 1.74 1.84 3.27 0.14 -2.20 -6.56 -3.89 -2.68 2.06 -2.37 -3.41 -1.86 1.20 1.95
Cytoplasmic Foci -0.44 0.06 -1.94 -1.68 -2.08 -2.04 -2.56 -3.34 0.51 1.19 -2.19 -3.11 -3.67 -0.89 -0.67
Eisosomes 1.53 -2.13 3.54 1.69 7.90 -3.40 -9.18 -3.36 -0.22 2.72 -2.58 -9.83 -1.89 0.94 7.04
Endoplasmic Reticulum 8.18 8.70 7.50 -2.09 -3.31 1.23 11.36 2.59 0.72 -7.05 4.55 14.42 7.93 1.35 -7.65
Endosome 0.12 4.61 -1.41 -1.31 -3.24 -1.95 -1.43 -1.62 0.49 0.22 -1.97 -1.15 -2.66 0.28 -0.71
Golgi -2.06 -4.18 -1.67 -1.17 -1.34 -2.27 -2.12 -1.52 -0.52 -0.06 -2.70 -2.77 -2.36 -1.11 -0.91
Lipid Particles -0.51 0.46 -1.59 -1.42 -1.69 -2.14 -2.88 -2.20 1.13 0.08 -2.25 -2.68 -2.38 -0.23 -1.56
Mitochondria -0.53 -7.69 -8.32 -5.26 -2.71 1.21 -12.21 -11.48 -11.74 -5.43 0.22 -15.75 -15.37 -13.89 -5.86
Mitotic Spindle -1.70 -3.15 -5.26 -1.91 -2.33 -2.34 -3.81 -3.38 -2.52 1.67 -2.23 -4.98 -6.14 -3.89 -1.14
None -3.04 0.57 1.45 3.31 1.51 -2.48 -9.20 -4.23 -0.31 5.99 -3.55 -7.08 -2.72 3.07 5.45
Nuclear Periphery 1.25 -0.79 -3.70 -5.28 -3.54 1.05 0.94 -3.82 -4.70 -4.93 1.56 -0.30 -5.40 -6.73 -5.57
Nuclear Periphery Foci -1.54 1.76 -1.01 0.97 -2.04 -2.40 -2.00 -2.61 0.22 -1.43 -2.77 -0.54 -2.62 0.68 -2.43
Nucleolus -1.94 -2.19 0.52 2.73 3.50 0.05 3.11 3.84 3.70 0.81 -0.80 1.05 3.84 4.61 3.10
Nucleus 2.46 3.45 4.46 0.77 1.32 1.35 6.12 6.71 4.98 1.19 2.73 7.73 9.02 5.14 1.85
Peroxisomes -0.10 -3.34 0.48 0.70 4.63 -2.63 -5.32 -2.80 -0.31 3.43 -2.23 -7.90 -2.70 -0.55 5.61
Vacuole -0.44 -0.41 -1.07 -0.49 -0.30 -2.66 -3.41 -3.44 -0.08 -0.69 -2.66 -1.95 -3.26 -0.52 -0.57
Vacuole Periphery -3.36 -0.65 -2.13 -0.11 -1.56 -2.46 4.63 -1.61 -0.49 -1.88 -3.10 0.93 -2.26 -0.28 -2.41
Standard name
Human Ortholog
Description Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Pre6

Pre6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available