Standard name
Human Ortholog
Description Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.96 0.96 1.0 0.99 0.97 0.97 0.97 0.96 0.95 0.92 0.92 0.99 0.99 1.0 0.91 0.96 0.88 0.47 0.58 0.65 0.77 0.67 0.64
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0 0 0.06 0.06 0.11 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0.06 0.38 0.32 0.19 0.17 0.24 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 0 3 0
Bud 1 2 2 5 3 10 18 21 10 19 19 29 0 0 0 1 0 1 1 4 1 0 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 2 2 0 2 6
Bud Site 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 1 1 1 2 3 1 4 4 8 3 3 2 3 2 0 0 0 0 1 0 0
Cytoplasm 72 186 288 254 320 357 567 667 392 550 365 507 322 448 512 144 90 61 69 111 189 207 309 353
Endoplasmic Reticulum 0 3 1 0 1 1 1 0 0 0 0 1 2 2 1 3 6 4 10 0 10 1 2 7
Endosome 0 0 1 0 0 3 3 7 0 1 2 4 0 1 0 1 1 0 1 4 9 2 8 10
Golgi 0 0 2 0 0 0 0 0 1 0 0 0 0 1 0 2 0 2 2 0 2 0 1 0
Mitochondria 5 2 3 0 3 9 20 18 23 37 45 70 0 2 0 1 0 1 3 3 7 2 2 10
Nucleus 0 2 0 0 0 1 1 2 0 1 1 4 1 0 0 3 1 0 0 0 0 0 2 4
Nuclear Periphery 5 2 0 0 0 2 0 4 1 0 0 1 0 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 0 0 0 0 1 0 1 3 0 0 5 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 2 0 1 0 1 0 0 0 0 0 0 0 3 4
Vac/Vac Membrane 4 1 9 0 1 2 6 17 10 7 10 10 1 1 2 18 5 4 55 61 55 44 112 145
Unique Cell Count 83 194 299 254 324 369 585 691 408 579 395 554 325 454 514 159 94 69 148 192 290 270 465 556
Labelled Cell Count 88 198 308 260 330 389 619 743 439 621 453 635 331 459 519 176 105 73 148 192 290 270 465 556


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.3 5.9 4.7 5.3 4.7 4.5 4.5 4.3 4.1 4.0 3.9 6.2 5.9 5.9 7.2 7.2 6.8 6.3 6.5 6.6
Std Deviation (1e-4) 0.5 1.0 5.9 1.7 1.7 1.5 1.4 1.3 1.4 0.9 1.5 1.5 1.0 0.9 1.1 1.8 1.1 1.6 1.2 1.4 1.4
Intensity Change (Log2) – – – -0.31 -0.15 -0.32 -0.39 -0.38 -0.47 -0.52 -0.57 -0.6 0.08 0.0 -0.01 0.29 0.28 0.21 0.09 0.15 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.1 2.0 0.3 0.5 0.2 -0.2 -0.9 -2.2 -2.7 2.3 2.1 3.6 -2.5 -0.3 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 -2.2 -0.5 -0.5 -1.9 -0.4 -1.1 0 0 0 3.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2101 4.6595 4.6468 4.8911 3.9836 4.6997 1.1626 2.1794 1.4193 1.3517 1.1911 1.4289 0.3019 0.8988 0.5549 0.9413 0.7223 0.7212
Actin 0.0032 0.0008 0.0059 0.0002 0.0003 0.0016 0.0433 0.0025 0.0113 0.0265 0.0104 0.0017 0.0454 0.0093 0.0133 0 0 0.0012
Bud 0.0005 0.001 0.0008 0.0006 0.0002 0.0012 0.0021 0.0024 0.0005 0.0012 0.0012 0.0008 0.0011 0.0003 0.0029 0.0003 0.0002 0.0005
Bud Neck 0.0019 0.004 0.0014 0.0003 0.0004 0.0019 0.005 0.0034 0.001 0.0015 0.002 0.0018 0.0028 0.0004 0.0004 0.0008 0.0003 0.0008
Bud Periphery 0.001 0.0015 0.0016 0.0005 0.0001 0.002 0.004 0.0018 0.0008 0.0021 0.0017 0.0012 0.0022 0.0002 0.0024 0.0002 0.0001 0.0002
Bud Site 0.0012 0.0033 0.0045 0.0007 0.0005 0.0009 0.0069 0.013 0.0018 0.0035 0.0014 0.0006 0.0027 0.0015 0.0026 0.0005 0.0002 0.0001
Cell Periphery 0.0009 0.0018 0.0006 0.0002 0.0004 0.0003 0.0008 0.0004 0.0003 0.0004 0.0003 0.0003 0.0005 0.0003 0.0003 0.0001 0.0001 0.0002
Cytoplasm 0.4986 0.6956 0.6021 0.5663 0.6285 0.6798 0.3985 0.7761 0.7459 0.652 0.7057 0.7259 0.5152 0.7162 0.6838 0.5924 0.734 0.6969
Cytoplasmic Foci 0.0317 0.0154 0.0251 0.0177 0.0333 0.0323 0.0692 0.0159 0.0213 0.051 0.0148 0.0159 0.0341 0.0209 0.0254 0.0123 0.02 0.028
Eisosomes 0.0001 0.0001 0.0003 0 0 0 0.0003 0 0.0002 0.0002 0.0002 0 0.0005 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0196 0.0144 0.0116 0.0082 0.0088 0.0115 0.0169 0.0112 0.0244 0.0096 0.0088 0.0197 0.0327 0.0114 0.0217 0.0191 0.0094 0.0154
Endosome 0.0697 0.0351 0.0569 0.0454 0.0766 0.0375 0.1221 0.0373 0.0673 0.0991 0.0397 0.0721 0.0666 0.0339 0.0686 0.0929 0.0774 0.0775
Golgi 0.0017 0.0009 0.0026 0.0006 0.0008 0.002 0.022 0.0029 0.0056 0.0153 0.0054 0.0055 0.008 0.0022 0.0037 0.0005 0.0006 0.001
Lipid Particles 0.0048 0.0022 0.0042 0.0005 0.0008 0.0028 0.0313 0.0018 0.0096 0.0162 0.001 0.0069 0.0081 0.0016 0.0059 0.0003 0.0002 0.0007
Mitochondria 0.0008 0.0006 0.0022 0.0005 0.0003 0.0045 0.009 0.0009 0.0049 0.0263 0.0587 0.0071 0.0073 0.0002 0.0019 0.0003 0.0002 0.0005
None 0.2647 0.1492 0.2045 0.2393 0.1609 0.16 0.1228 0.0803 0.0573 0.015 0.0069 0.0286 0.1845 0.1141 0.1078 0.0412 0.0839 0.0543
Nuclear Periphery 0.0113 0.0036 0.0107 0.0027 0.0029 0.0078 0.028 0.0028 0.0102 0.0085 0.0039 0.0079 0.0135 0.0031 0.0069 0.009 0.0032 0.0168
Nucleolus 0.0012 0.0005 0.0006 0.0003 0.0002 0.0002 0.003 0.0003 0.0004 0.0016 0.0003 0.0002 0.0009 0.0002 0.0004 0.0002 0.0001 0.0002
Nucleus 0.0072 0.0049 0.0047 0.0039 0.0036 0.0025 0.02 0.0029 0.0038 0.0038 0.0092 0.0049 0.0058 0.0032 0.003 0.004 0.0024 0.0044
Peroxisomes 0.0006 0.001 0.0019 0.0002 0.0002 0.0013 0.0228 0.0003 0.0022 0.0063 0.0006 0.0008 0.0082 0.0009 0.0029 0 0 0.0001
Punctate Nuclear 0.0106 0.0021 0.0019 0.001 0.0037 0.0021 0.0101 0.0007 0.0018 0.0048 0.0004 0.0007 0.003 0.0011 0.0034 0.0003 0.0003 0.0015
Vacuole 0.0657 0.06 0.0516 0.1049 0.0743 0.0448 0.0544 0.0407 0.0265 0.0463 0.1231 0.0905 0.0528 0.0767 0.0389 0.2122 0.0612 0.0941
Vacuole Periphery 0.0032 0.0022 0.0043 0.0062 0.0032 0.0031 0.0075 0.0022 0.0031 0.0089 0.0042 0.007 0.0041 0.0025 0.0035 0.0135 0.0061 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.9681 21.0365 10.9848 18.8709 18.6454 15.6795 23.8302 19.9386 19.0115 20.9906
Translational Efficiency 1.3803 1.0535 1.7078 0.9365 1.3046 2.2131 0.9184 1.1272 1.3075 1.2994

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1270 541 1702 221 1712 1745 125 145 2982 2286 1827 366

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 717.91 719.38 936.24 919.40 727.73 767.85 1095.81 911.12 723.55 756.38 947.16 916.12
Standard Deviation 102.00 127.43 117.27 136.50 100.87 98.28 177.26 104.58 101.47 107.89 128.78 124.90
Intensity Change Log 2 — 0.002951 0.383075 0.356890 — 0.077421 0.590522 0.324238 — 0.040919 0.491228 0.340545

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000194 0.000849 0.001118 0.010225 0.000264 0.000325 0.000938 0.004060 0.000234 0.000449 0.001106 0.007782
Bud Neck 0.020561 0.023312 0.005601 0.003746 0.014155 0.023214 0.004724 0.002584 0.016883 0.023237 0.005541 0.003285
Bud Site 0.007352 0.022116 0.011062 0.031508 0.007070 0.016057 0.008493 0.030852 0.007190 0.017491 0.010887 0.031248
Cell Periphery 0.000234 0.000130 0.000420 0.000437 0.000139 0.000156 0.000548 0.000312 0.000179 0.000150 0.000429 0.000387
Cytoplasm 0.417312* 0.401535* 0.523286* 0.370090* 0.530340* 0.411008* 0.355780* 0.439390* 0.482203* 0.408766* 0.511825* 0.397545*
Cytoplasmic Foci 0.189477* 0.227302* 0.030995 0.052583 0.129280 0.166102 0.025646 0.038870 0.154917 0.180585* 0.030629 0.047151
Eisosomes 0.000259 0.000137 0.000093 0.000169 0.000149 0.000140 0.000161 0.000127 0.000196 0.000140 0.000098 0.000152
Endoplasmic Reticulum 0.001889 0.000991 0.009154 0.008610 0.000991 0.001507 0.013242 0.003314 0.001373 0.001385 0.009434 0.006511
Endosome 0.042063 0.050251 0.004613 0.007583 0.020281 0.044245 0.008779 0.013479 0.029558 0.045666 0.004898 0.009919
Golgi 0.002236 0.003151 0.000752 0.017479 0.001508 0.003837 0.000144 0.011886 0.001818 0.003675 0.000711 0.015263
Lipid Particles 0.010479 0.005526 0.003628 0.004864 0.004074 0.005903 0.006187 0.001823 0.006802 0.005814 0.003803 0.003659
Mitochondria 0.001965 0.001336 0.000366 0.008418 0.000923 0.003954 0.000182 0.025993 0.001366 0.003334 0.000353 0.015381
Mitotic Spindle 0.003151 0.001332 0.001548 0.005054 0.001668 0.008451 0.000792 0.008113 0.002300 0.006766 0.001496 0.006266
None 0.001786 0.002685 0.002364 0.001705 0.001151 0.001590 0.002046 0.002312 0.001421 0.001849 0.002343 0.001946
Nuclear Periphery 0.000382 0.000345 0.001908 0.001010 0.000260 0.000399 0.002004 0.000658 0.000312 0.000386 0.001915 0.000871
Nuclear Periphery Foci 0.001119 0.001323 0.006291 0.009435 0.000787 0.000653 0.011384 0.002997 0.000928 0.000811 0.006639 0.006885
Nucleolus 0.000678 0.001542 0.000274 0.000171 0.000529 0.001331 0.000249 0.000138 0.000592 0.001381 0.000272 0.000158
Nucleus 0.047480 0.038179 0.254957* 0.094078 0.066493 0.058026 0.192799* 0.088963 0.058395 0.053329 0.250704* 0.092052
Peroxisomes 0.002998 0.014879 0.000645 0.000551 0.003079 0.007351 0.000624 0.002279 0.003044 0.009133 0.000643 0.001236
Vacuole 0.246767* 0.200924* 0.140768 0.370170* 0.216126* 0.243698* 0.365121* 0.318387* 0.229176* 0.233576* 0.156118 0.349655*
Vacuole Periphery 0.001619 0.002156 0.000159 0.002114 0.000735 0.002053 0.000158 0.003463 0.001112 0.002077 0.000159 0.002649

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -1.72 -1.55 -1.36 -1.30 -0.84 -3.34 -1.60 -1.53 -0.67 -3.23 -1.74 -1.77 -1.66 -1.36
Bud Neck -0.67 9.10 10.68 6.97 3.33 -5.44 6.90 11.20 14.95 4.43 -4.18 11.05 15.03 15.99 4.78
Bud Site -6.84 -2.11 -2.48 0.30 -1.81 -5.81 -2.46 -1.58 0.02 -0.84 -8.09 -2.57 -2.96 -0.27 -1.97
Cell Periphery 5.92 -7.42 -3.13 -5.95 1.36 -0.95 -7.67 -1.47 -0.75 5.96 2.11 -12.02 -4.16 -4.92 4.29
Cytoplasm 1.10 -6.11 6.06 4.69 9.24 12.68 12.12 7.49 2.58 -3.30 9.46 2.43 10.75 5.80 9.05
Cytoplasmic Foci -3.87 33.20 24.01 20.37 -2.57 -6.89 26.58 18.64 23.40 -2.78 -5.35 41.33 28.89 30.18 -2.69
Eisosomes 6.72 11.05 5.41 -0.71 -3.32 0.52 -1.14 3.24 2.64 4.26 4.44 9.66 4.99 0.66 -2.53
Endoplasmic Reticulum 3.81 -9.64 -6.54 -7.72 1.75 -1.05 -6.49 -4.60 -2.00 5.35 -0.01 -11.43 -7.75 -6.47 4.51
Endosome -1.79 18.46 17.57 12.47 -1.53 -10.72 9.12 6.59 13.78 1.03 -7.79 22.67 17.69 20.88 -0.76
Golgi -1.13 2.67 -0.97 -0.79 -1.34 -3.78 4.79 -0.77 0.28 -1.41 -3.62 2.64 -1.21 -0.56 -1.64
Lipid Particles 4.21 6.92 5.98 1.52 -1.47 -2.52 -2.27 7.30 5.92 5.44 1.50 6.13 6.91 4.18 1.53
Mitochondria 1.33 3.66 -0.41 -0.63 -0.94 -3.99 4.22 -1.40 -0.70 -1.57 -3.23 4.74 -1.33 -0.55 -1.73
Mitotic Spindle 1.06 0.82 -0.23 -1.33 -1.10 -3.92 0.37 -1.51 1.42 -1.71 -3.25 0.44 -1.34 1.67 -1.72
None -0.76 -0.60 0.44 0.98 1.09 -0.77 -1.06 -1.55 -0.87 -1.04 -0.79 -1.95 -1.04 -0.10 0.31
Nuclear Periphery 0.47 -14.07 -7.38 -7.08 5.72 -3.27 -12.39 -7.32 -5.83 6.36 -1.94 -16.32 -10.52 -9.32 7.47
Nuclear Periphery Foci -0.83 -8.14 -7.53 -7.48 -1.75 1.21 -8.25 -6.63 -7.04 5.99 0.57 -10.58 -9.07 -9.47 0.60
Nucleolus -3.06 6.82 9.27 4.95 3.97 -2.54 5.45 8.01 3.72 1.69 -3.16 8.35 12.73 4.94 4.14
Nucleus 2.12 -37.11 -5.82 -6.45 17.37 2.69 -6.70 -3.45 -4.17 3.53 2.01 -36.51 -6.12 -6.74 18.82
Peroxisomes -5.52 8.49 11.15 6.80 0.86 -4.60 4.23 2.94 7.56 -0.82 -7.35 6.51 6.23 11.09 -0.21
Vacuole 3.93 8.13 -11.39 -12.86 -14.67 -3.64 -10.00 -9.22 -8.15 1.18 -0.90 4.04 -15.03 -14.53 -16.27
Vacuole Periphery -1.28 11.04 1.47 2.03 -1.39 -7.56 7.97 -0.33 1.47 -1.15 -5.83 13.39 0.51 2.63 -1.80
Standard name
Human Ortholog
Description Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein
Localization
Cell Percentages vacuole (37%), cytoplasm (14%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sil1

Sil1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sil1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available