Standard name
Human Ortholog
Description Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.61 0.44 0.74 0.74 0.81 0.79 0.84 0.81 0.89 0.85 0.4 0.35 0.4 0.71 0.58 0.67 0.11 0.08 0 0.05 0 0
Endoplasmic Reticulum 0.3 0.43 0.5 0.3 0.25 0.18 0.19 0.11 0.14 0 0.09 0.64 0.67 0.57 0 0 0 0.6 0.72 0.82 0.63 0.69 0.66
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.06 0.11 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0.32 0.44 0.36 0.22 0.13 0.09 0.22 0.22 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 5 1 7 3 1 8 13 10 9 3 6 9 1 0 1 1 2 0 1 2 2
Bud 2 1 0 4 4 17 11 18 16 28 27 0 1 2 0 0 0 3 2 1 0 5 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 1 8 11 14 24 11 26 26 25 1 5 11 3 2 2 0 0 0 0 0 0
Cytoplasm 263 236 141 214 261 444 492 516 582 496 579 123 151 161 130 93 83 54 33 8 7 12 10
Endoplasmic Reticulum 136 166 160 87 89 97 118 66 99 16 60 196 285 228 1 4 4 300 304 279 95 262 234
Endosome 0 0 5 2 1 0 1 4 3 3 0 3 3 5 0 0 0 15 13 9 3 5 7
Golgi 2 0 2 0 0 0 0 0 0 0 0 1 2 8 0 3 2 2 1 2 2 1 4
Mitochondria 2 0 0 0 1 0 1 4 2 7 2 0 0 0 2 0 0 0 2 1 1 1 1
Nucleus 0 0 0 0 1 0 0 3 1 1 2 1 0 1 5 0 0 1 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 2 3 0 0 2 0 0 0 0 0 0 9 4 3 3 1 2
Nucleolus 0 0 2 1 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 89 23 36 8 11 18 20 26 54 30 39 7 18 16 58 71 44 110 53 32 34 83 86
Unique Cell Count 449 386 319 290 354 546 622 614 717 557 683 306 427 399 183 161 123 505 423 342 153 381 357
Labelled Cell Count 495 427 352 326 386 593 670 660 796 618 745 335 472 441 200 173 136 505 423 342 153 381 357


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.1 23.7 16.9 16.8 16.1 14.2 13.5 13.2 11.5 11.2 11.3 20.0 20.0 18.6 25.5 25.5 26.1 20.9 21.1 21.5
Std Deviation (1e-4) 4.9 4.5 3.4 3.4 3.2 2.7 2.7 2.7 2.5 2.6 2.5 4.1 4.2 4.1 6.9 6.9 6.1 5.6 4.7 5.0
Intensity Change (Log2) -0.01 -0.07 -0.25 -0.32 -0.36 -0.55 -0.6 -0.58 0.24 0.24 0.13 0.59 0.59 0.62 0.3 0.32 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0.4 0 0 0 0.3 0.3 0 0 0 0.7 0 0 0
Bud 0 0 3.2 0 3.1 0 4.1 3.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.7 2.4 3.2 0 3.1 3.6 3.1 0 0 2.5 0 0 0
Cytoplasm 7.4 7.8 11.3 10.8 12.6 12.0 14.3 13.3 -1.0 -2.4 -1.0 5.8 2.8 4.4
Endoplasmic Reticulum -5.1 -6.7 -10.1 -9.9 -13.3 -12.5 -16.8 -14.7 3.5 4.6 1.9 -11.5 -10.4 -9.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.1 -4.2 -4.7 -5.0 -4.1 -2.0 -3.2 -3.1 -4.4 -3.7 -3.7 5.6 8.2 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.1869 16.179 15.1888 14.0549 14.4626 14.9207 15.3418 17.4498 16.8926 15.7631 15.3873 15.9897 14.4384 17.1311 16.537 14.8424 13.9643 15.9055
Actin 0.0001 0.0001 0 0 0 0 0.0067 0.0001 0.0001 0.0237 0.0178 0.0002 0.0059 0.0001 0.0057 0.0003 0.0031 0
Bud 0.0001 0.0001 0.0001 0.0001 0.001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0006 0.0005 0.0003 0.0007 0.0008 0.0002 0.0003 0.0004 0.0019 0.0018 0.0005 0.0004 0.0008 0.001 0.0009 0.0004 0.0009
Bud Periphery 0.0003 0.0003 0.0004 0.0005 0.0017 0.0003 0.0002 0.0003 0.0004 0.0006 0.001 0.0003 0.0003 0.0002 0.0004 0.0003 0.0003 0.0004
Bud Site 0.0002 0.0004 0.0003 0.0002 0.001 0.0001 0.0002 0.0003 0.0008 0.0007 0.0091 0.0001 0.0005 0.0004 0.0031 0.0004 0.0001 0.0001
Cell Periphery 0.0057 0.0088 0.0077 0.0064 0.0023 0.0022 0.002 0.0047 0.0033 0.0016 0.0012 0.0016 0.0059 0.0075 0.0054 0.0019 0.0111 0.0059
Cytoplasm 0.064 0.0998 0.0758 0.0638 0.0771 0.0843 0.0395 0.0691 0.0707 0.0243 0.0834 0.0463 0.0522 0.1079 0.0696 0.0998 0.0525 0.0509
Cytoplasmic Foci 0.0026 0.0021 0.0025 0.0013 0.0031 0.0017 0.0051 0.0031 0.0041 0.0041 0.009 0.0033 0.0071 0.0032 0.0019 0.0072 0.0186 0.0017
Eisosomes 0 0 0 0 0 0 0 0 0 0.0014 0.0004 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.415 0.4221 0.4267 0.2342 0.1966 0.43 0.5153 0.518 0.5412 0.2792 0.3284 0.5097 0.5104 0.4875 0.3908 0.2294 0.3907 0.3758
Endosome 0.0598 0.0348 0.0508 0.0319 0.0715 0.0432 0.0896 0.0585 0.0741 0.0682 0.1464 0.0883 0.0417 0.0205 0.0431 0.0611 0.0599 0.0508
Golgi 0.001 0.0007 0.0014 0.0002 0.0007 0.0007 0.008 0.0018 0.0028 0.0056 0.005 0.0027 0.0024 0.0007 0.0017 0.0068 0.0019 0.0007
Lipid Particles 0.0102 0.0073 0.0091 0.0043 0.0062 0.0035 0.0231 0.0156 0.009 0.0096 0.0023 0.0119 0.0106 0.0107 0.0042 0.0447 0.0145 0.0086
Mitochondria 0.0004 0.0001 0.0003 0.0002 0.0003 0.0003 0.0024 0.0004 0.0009 0.0016 0.0014 0.0014 0.001 0.0001 0.0082 0.0029 0.0006 0.0003
None 0.0034 0.0002 0.0002 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002 0.0064 0.0004 0.0002 0.0029 0.0001 0.0001 0.0002 0.0052 0.0001
Nuclear Periphery 0.0671 0.0464 0.0466 0.064 0.0439 0.0893 0.0799 0.0557 0.0437 0.0403 0.0518 0.0812 0.0931 0.0429 0.0609 0.0572 0.0734 0.0975
Nucleolus 0.0004 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.0012 0.0002 0.0001 0.0002 0.0002 0.0001
Nucleus 0.0075 0.0072 0.004 0.0062 0.0074 0.0117 0.0036 0.004 0.0031 0.0024 0.0053 0.0048 0.0069 0.0039 0.0064 0.0082 0.0046 0.0085
Peroxisomes 0 0 0 0 0 0 0.0004 0 0 0.0029 0.0005 0.0001 0.0037 0 0.0021 0.0036 0.0041 0
Punctate Nuclear 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0004 0.0002 0.0002 0.0022 0.0026 0.0002 0.0022 0.0002 0.0001 0.0004 0.005 0.0001
Vacuole 0.3442 0.3592 0.3479 0.5593 0.5476 0.3086 0.2034 0.2528 0.2218 0.4595 0.2826 0.2128 0.228 0.3007 0.3706 0.4522 0.3346 0.3608
Vacuole Periphery 0.0176 0.0095 0.0252 0.0266 0.0384 0.0228 0.0196 0.0148 0.0229 0.0629 0.0488 0.0344 0.0234 0.0122 0.0243 0.0223 0.0189 0.0366

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 126.5372 63.3453 48.1241 62.0707 98.4366 60.7042 80.8229 59.6962 53.7258 100.6411
Translational Efficiency 1.2577 1.6409 1.5828 0.8701 1.0916 2.9572 1.329 1.1597 1.4605 1.3466

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1506 1379 1608 1580 1820 1774 2162 1176 3326 3153 3770 2756

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1160.71 1665.23 1993.94 2824.62 1391.56 1572.25 2208.72 2791.84 1287.03 1612.92 2117.11 2810.63
Standard Deviation 234.88 325.63 341.99 660.89 391.87 307.42 370.85 587.19 349.59 318.86 374.22 630.71
Intensity Change Log 2 0.520714 0.780614 1.283049 0.176128 0.666507 1.004513 0.343090 0.719522 1.137880

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000380 0.000198 0.000102 0.000572 0.000115 0.000127 0.000213 0.000507 0.000235 0.000158 0.000166 0.000545
Bud Neck 0.000836 0.000727 0.001608 0.001896 0.003043 0.000598 0.000608 0.001290 0.002044 0.000655 0.001034 0.001637
Bud Site 0.005452 0.002768 0.001618 0.005491 0.010128 0.003366 0.001679 0.005090 0.008011 0.003105 0.001653 0.005320
Cell Periphery 0.009153 0.003626 0.006900 0.048082 0.003354 0.004088 0.011521 0.049774 0.005979 0.003886 0.009550 0.048804
Cytoplasm 0.042114 0.021338 0.009158 0.010157 0.044134 0.014250 0.008870 0.006437 0.043219 0.017350 0.008993 0.008570
Cytoplasmic Foci 0.015573 0.009191 0.002751 0.001346 0.012308 0.016721 0.002555 0.001861 0.013786 0.013428 0.002639 0.001565
Eisosomes 0.000073 0.000014 0.000013 0.000147 0.000059 0.000021 0.000031 0.000203 0.000065 0.000018 0.000023 0.000171
Endoplasmic Reticulum 0.576279* 0.493420* 0.281497* 0.721733* 0.367883* 0.550093* 0.684108* 0.742096* 0.462244* 0.525306* 0.512384* 0.730422*
Endosome 0.024705 0.047726 0.034627 0.013404 0.027749 0.064848 0.027844 0.012593 0.026371 0.057359 0.030737 0.013058
Golgi 0.005469 0.009924 0.017039 0.009256 0.004390 0.015250 0.008941 0.015459 0.004879 0.012920 0.012395 0.011903
Lipid Particles 0.012589 0.011522 0.019585 0.076569 0.005580 0.019538 0.027671 0.093823 0.008754 0.016032 0.024222 0.083932
Mitochondria 0.000778 0.000534 0.000250 0.000933 0.000452 0.000838 0.000485 0.000589 0.000600 0.000705 0.000385 0.000786
Mitotic Spindle 0.000041 0.000308 0.000302 0.000309 0.000195 0.000163 0.000077 0.000418 0.000125 0.000226 0.000173 0.000356
None 0.000884 0.000294 0.000218 0.000749 0.008782 0.000351 0.000750 0.000527 0.005206 0.000326 0.000523 0.000654
Nuclear Periphery 0.000421 0.002421 0.007134 0.002673 0.001898 0.001055 0.001463 0.002371 0.001229 0.001653 0.003881 0.002544
Nuclear Periphery Foci 0.004129 0.022829 0.065920 0.009741 0.017061 0.011487 0.014808 0.006677 0.011206 0.016448 0.036608 0.008434
Nucleolus 0.000139 0.000186 0.000059 0.000034 0.003279 0.000075 0.000035 0.000028 0.001857 0.000124 0.000045 0.000031
Nucleus 0.000592 0.000896 0.000299 0.000421 0.007729 0.000274 0.000164 0.000371 0.004497 0.000546 0.000222 0.000399
Peroxisomes 0.000632 0.000097 0.000026 0.000288 0.000317 0.000173 0.000195 0.000358 0.000460 0.000140 0.000123 0.000318
Vacuole 0.281209* 0.338985* 0.505578* 0.090529 0.442203* 0.270170* 0.198743 0.055866 0.369306* 0.300267* 0.329616* 0.075738
Vacuole Periphery 0.018553 0.032995 0.045316 0.005668 0.039341 0.026515 0.009240 0.003663 0.029928 0.029349 0.024627 0.004812

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.29 1.90 -1.26 -2.52 -2.99 -0.61 -0.80 -4.29 -4.16 -2.08 1.18 0.49 -3.21 -4.23 -3.26
Bud Neck 0.84 -5.24 -3.96 -4.30 -1.26 4.57 4.50 2.55 -3.92 -3.78 4.64 3.13 0.39 -5.77 -3.64
Bud Site 2.74 3.81 -0.73 -3.20 -4.09 4.85 6.25 2.25 -2.20 -3.61 5.54 7.29 1.45 -3.71 -5.42
Cell Periphery 7.36 3.01 -16.01 -18.91 -17.47 -1.90 -11.90 -16.43 -16.15 -13.31 5.19 -6.57 -23.41 -24.83 -21.39
Cytoplasm 6.39 12.07 11.23 4.63 -0.67 9.84 12.34 12.76 4.56 1.85 11.70 17.17 16.61 5.99 0.27
Cytoplasmic Foci 3.90 8.95 9.84 9.34 4.48 -2.37 9.36 9.82 10.40 1.45 0.44 12.90 14.04 13.47 3.67
Eisosomes 1.96 2.00 -2.25 -8.06 -8.14 1.50 1.13 -2.10 -2.83 -2.69 2.44 2.18 -3.07 -5.24 -5.07
Endoplasmic Reticulum 6.40 24.73 -11.20 -17.79 -38.40* -16.07 -29.84 -31.75 -15.51 -5.26 -7.07 -5.22 -30.36 -22.99 -25.11
Endosome -10.28 -6.29 8.10 16.48 15.94 -14.48 -0.27 10.02 22.65 13.48 -17.75 -4.03 12.70 27.75 20.29
Golgi -3.83 -9.81 -4.69 0.50 6.06 -8.28 -5.83 -8.48 -0.12 -4.83 -8.97 -11.10 -9.64 0.95 0.45
Lipid Particles 0.59 -4.07 -15.65 -15.81 -13.76 -8.25 -11.94 -17.21 -13.94 -12.28 -5.98 -11.86 -23.55 -20.60 -17.90
Mitochondria 1.37 5.03 -0.59 -1.30 -2.40 -1.62 -0.39 -1.58 0.75 -1.14 -0.68 3.12 -1.24 -0.52 -2.43
Mitotic Spindle -2.00 -3.21 -5.37 -0.05 -0.07 0.41 1.82 -1.53 -1.64 -1.92 -1.43 -1.09 -2.48 -1.62 -2.08
None 1.15 1.31 0.17 -2.50 -2.97 5.42 5.15 5.28 -1.42 0.76 5.52 5.27 5.07 -2.74 -1.09
Nuclear Periphery -7.64 -15.28 -18.59 -0.90 9.94 2.68 1.25 -1.40 -8.11 -5.48 -2.06 -10.50 -6.96 -5.88 6.48
Nuclear Periphery Foci -10.44 -20.43 -6.33 6.44 17.88 3.16 0.92 5.94 4.69 6.64 -3.96 -14.67 2.35 7.26 17.70
Nucleolus -0.45 4.41 5.73 1.43 5.81 6.73 6.82 6.83 6.80 1.36 6.52 6.91 6.96 1.95 4.31
Nucleus -0.79 2.13 0.58 1.03 -1.62 6.30 6.39 6.01 -1.93 -2.63 5.85 6.52 6.04 0.13 -3.30
Peroxisomes 6.61 7.83 4.04 -4.20 -6.84 3.47 2.86 -0.34 -2.27 -2.00 7.39 7.85 2.61 -4.12 -4.59
Vacuole -5.07 -20.47 20.41 26.43 47.55* 17.29 25.94 47.49* 28.26 21.90 8.89 4.80 46.05* 37.08 42.77*
Vacuole Periphery -7.14 -12.75 10.34 15.22 21.37* 6.40 18.12 21.92 17.81 9.18 0.42 4.27 23.22 22.69 21.33*
Standard name
Human Ortholog
Description Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
Localization
Cell Percentages ER (36%), vacuole (4%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Phs1

Phs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Phs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available