Standard name | |
---|---|
Human Ortholog | |
Description | Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 5 | 1 | 7 | 3 | 1 | 8 | – | 13 | 10 | 9 | 3 | 6 | 9 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 2 | 2 |
Bud | 2 | 1 | 0 | 4 | 4 | 17 | 11 | 18 | – | 16 | 28 | 27 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 5 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 8 | 11 | 14 | 24 | 11 | – | 26 | 26 | 25 | 1 | 5 | 11 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 263 | 236 | 141 | 214 | 261 | 444 | 492 | 516 | – | 582 | 496 | 579 | 123 | 151 | 161 | 130 | 93 | 83 | 54 | 33 | 8 | 7 | 12 | 10 |
Endoplasmic Reticulum | 136 | 166 | 160 | 87 | 89 | 97 | 118 | 66 | – | 99 | 16 | 60 | 196 | 285 | 228 | 1 | 4 | 4 | 300 | 304 | 279 | 95 | 262 | 234 |
Endosome | 0 | 0 | 5 | 2 | 1 | 0 | 1 | 4 | – | 3 | 3 | 0 | 3 | 3 | 5 | 0 | 0 | 0 | 15 | 13 | 9 | 3 | 5 | 7 |
Golgi | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 8 | 0 | 3 | 2 | 2 | 1 | 2 | 2 | 1 | 4 |
Mitochondria | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | – | 2 | 7 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 1 | 1 | 2 | 1 | 0 | 1 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 4 | 3 | 3 | 1 | 2 |
Nucleolus | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 89 | 23 | 36 | 8 | 11 | 18 | 20 | 26 | – | 54 | 30 | 39 | 7 | 18 | 16 | 58 | 71 | 44 | 110 | 53 | 32 | 34 | 83 | 86 |
Unique Cell Count | 449 | 386 | 319 | 290 | 354 | 546 | 622 | 614 | 717 | 557 | 683 | 306 | 427 | 399 | 183 | 161 | 123 | 505 | 423 | 342 | 153 | 381 | 357 | |
Labelled Cell Count | 495 | 427 | 352 | 326 | 386 | 593 | 670 | 660 | 796 | 618 | 745 | 335 | 472 | 441 | 200 | 173 | 136 | 505 | 423 | 342 | 153 | 381 | 357 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.1 | 23.7 | 16.9 | 16.8 | 16.1 | 14.2 | 13.5 | 13.2 | – | 11.5 | 11.2 | 11.3 | 20.0 | 20.0 | 18.6 | 25.5 | 25.5 | 26.1 | 20.9 | 21.1 | 21.5 |
Std Deviation (1e-4) | 4.9 | 4.5 | 3.4 | 3.4 | 3.2 | 2.7 | 2.7 | 2.7 | – | 2.5 | 2.6 | 2.5 | 4.1 | 4.2 | 4.1 | 6.9 | 6.9 | 6.1 | 5.6 | 4.7 | 5.0 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.07 | -0.25 | -0.32 | -0.36 | – | -0.55 | -0.6 | -0.58 | 0.24 | 0.24 | 0.13 | 0.59 | 0.59 | 0.62 | 0.3 | 0.32 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.4 | 0 | 0 | 0 | – | 0.3 | 0.3 | 0 | 0 | 0 | 0.7 | 0 | 0 | 0 |
Bud | 0 | 0 | 3.2 | 0 | 3.1 | – | 0 | 4.1 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.7 | 2.4 | 3.2 | 0 | – | 3.1 | 3.6 | 3.1 | 0 | 0 | 2.5 | 0 | 0 | 0 |
Cytoplasm | 7.4 | 7.8 | 11.3 | 10.8 | 12.6 | – | 12.0 | 14.3 | 13.3 | -1.0 | -2.4 | -1.0 | 5.8 | 2.8 | 4.4 |
Endoplasmic Reticulum | -5.1 | -6.7 | -10.1 | -9.9 | -13.3 | – | -12.5 | -16.8 | -14.7 | 3.5 | 4.6 | 1.9 | -11.5 | -10.4 | -9.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.1 | -4.2 | -4.7 | -5.0 | -4.1 | – | -2.0 | -3.2 | -3.1 | -4.4 | -3.7 | -3.7 | 5.6 | 8.2 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.1869 | 16.179 | 15.1888 | 14.0549 | 14.4626 | 14.9207 | 15.3418 | 17.4498 | 16.8926 | 15.7631 | 15.3873 | 15.9897 | 14.4384 | 17.1311 | 16.537 | 14.8424 | 13.9643 | 15.9055 |
Actin | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0067 | 0.0001 | 0.0001 | 0.0237 | 0.0178 | 0.0002 | 0.0059 | 0.0001 | 0.0057 | 0.0003 | 0.0031 | 0 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0019 | 0.0018 | 0.0005 | 0.0004 | 0.0008 | 0.001 | 0.0009 | 0.0004 | 0.0009 |
Bud Periphery | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0017 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0004 |
Bud Site | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0007 | 0.0091 | 0.0001 | 0.0005 | 0.0004 | 0.0031 | 0.0004 | 0.0001 | 0.0001 |
Cell Periphery | 0.0057 | 0.0088 | 0.0077 | 0.0064 | 0.0023 | 0.0022 | 0.002 | 0.0047 | 0.0033 | 0.0016 | 0.0012 | 0.0016 | 0.0059 | 0.0075 | 0.0054 | 0.0019 | 0.0111 | 0.0059 |
Cytoplasm | 0.064 | 0.0998 | 0.0758 | 0.0638 | 0.0771 | 0.0843 | 0.0395 | 0.0691 | 0.0707 | 0.0243 | 0.0834 | 0.0463 | 0.0522 | 0.1079 | 0.0696 | 0.0998 | 0.0525 | 0.0509 |
Cytoplasmic Foci | 0.0026 | 0.0021 | 0.0025 | 0.0013 | 0.0031 | 0.0017 | 0.0051 | 0.0031 | 0.0041 | 0.0041 | 0.009 | 0.0033 | 0.0071 | 0.0032 | 0.0019 | 0.0072 | 0.0186 | 0.0017 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0014 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.415 | 0.4221 | 0.4267 | 0.2342 | 0.1966 | 0.43 | 0.5153 | 0.518 | 0.5412 | 0.2792 | 0.3284 | 0.5097 | 0.5104 | 0.4875 | 0.3908 | 0.2294 | 0.3907 | 0.3758 |
Endosome | 0.0598 | 0.0348 | 0.0508 | 0.0319 | 0.0715 | 0.0432 | 0.0896 | 0.0585 | 0.0741 | 0.0682 | 0.1464 | 0.0883 | 0.0417 | 0.0205 | 0.0431 | 0.0611 | 0.0599 | 0.0508 |
Golgi | 0.001 | 0.0007 | 0.0014 | 0.0002 | 0.0007 | 0.0007 | 0.008 | 0.0018 | 0.0028 | 0.0056 | 0.005 | 0.0027 | 0.0024 | 0.0007 | 0.0017 | 0.0068 | 0.0019 | 0.0007 |
Lipid Particles | 0.0102 | 0.0073 | 0.0091 | 0.0043 | 0.0062 | 0.0035 | 0.0231 | 0.0156 | 0.009 | 0.0096 | 0.0023 | 0.0119 | 0.0106 | 0.0107 | 0.0042 | 0.0447 | 0.0145 | 0.0086 |
Mitochondria | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0024 | 0.0004 | 0.0009 | 0.0016 | 0.0014 | 0.0014 | 0.001 | 0.0001 | 0.0082 | 0.0029 | 0.0006 | 0.0003 |
None | 0.0034 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0064 | 0.0004 | 0.0002 | 0.0029 | 0.0001 | 0.0001 | 0.0002 | 0.0052 | 0.0001 |
Nuclear Periphery | 0.0671 | 0.0464 | 0.0466 | 0.064 | 0.0439 | 0.0893 | 0.0799 | 0.0557 | 0.0437 | 0.0403 | 0.0518 | 0.0812 | 0.0931 | 0.0429 | 0.0609 | 0.0572 | 0.0734 | 0.0975 |
Nucleolus | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0075 | 0.0072 | 0.004 | 0.0062 | 0.0074 | 0.0117 | 0.0036 | 0.004 | 0.0031 | 0.0024 | 0.0053 | 0.0048 | 0.0069 | 0.0039 | 0.0064 | 0.0082 | 0.0046 | 0.0085 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0029 | 0.0005 | 0.0001 | 0.0037 | 0 | 0.0021 | 0.0036 | 0.0041 | 0 |
Punctate Nuclear | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0022 | 0.0026 | 0.0002 | 0.0022 | 0.0002 | 0.0001 | 0.0004 | 0.005 | 0.0001 |
Vacuole | 0.3442 | 0.3592 | 0.3479 | 0.5593 | 0.5476 | 0.3086 | 0.2034 | 0.2528 | 0.2218 | 0.4595 | 0.2826 | 0.2128 | 0.228 | 0.3007 | 0.3706 | 0.4522 | 0.3346 | 0.3608 |
Vacuole Periphery | 0.0176 | 0.0095 | 0.0252 | 0.0266 | 0.0384 | 0.0228 | 0.0196 | 0.0148 | 0.0229 | 0.0629 | 0.0488 | 0.0344 | 0.0234 | 0.0122 | 0.0243 | 0.0223 | 0.0189 | 0.0366 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 126.5372 | 63.3453 | 48.1241 | 62.0707 | 98.4366 | 60.7042 | 80.8229 | 59.6962 | 53.7258 | 100.6411 |
Translational Efficiency | 1.2577 | 1.6409 | 1.5828 | 0.8701 | 1.0916 | 2.9572 | 1.329 | 1.1597 | 1.4605 | 1.3466 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1506 | 1379 | 1608 | 1580 | 1820 | 1774 | 2162 | 1176 | 3326 | 3153 | 3770 | 2756 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1160.71 | 1665.23 | 1993.94 | 2824.62 | 1391.56 | 1572.25 | 2208.72 | 2791.84 | 1287.03 | 1612.92 | 2117.11 | 2810.63 |
Standard Deviation | 234.88 | 325.63 | 341.99 | 660.89 | 391.87 | 307.42 | 370.85 | 587.19 | 349.59 | 318.86 | 374.22 | 630.71 |
Intensity Change Log 2 | — | 0.520714 | 0.780614 | 1.283049 | — | 0.176128 | 0.666507 | 1.004513 | — | 0.343090 | 0.719522 | 1.137880 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000380 | 0.000198 | 0.000102 | 0.000572 | 0.000115 | 0.000127 | 0.000213 | 0.000507 | 0.000235 | 0.000158 | 0.000166 | 0.000545 |
Bud Neck | 0.000836 | 0.000727 | 0.001608 | 0.001896 | 0.003043 | 0.000598 | 0.000608 | 0.001290 | 0.002044 | 0.000655 | 0.001034 | 0.001637 |
Bud Site | 0.005452 | 0.002768 | 0.001618 | 0.005491 | 0.010128 | 0.003366 | 0.001679 | 0.005090 | 0.008011 | 0.003105 | 0.001653 | 0.005320 |
Cell Periphery | 0.009153 | 0.003626 | 0.006900 | 0.048082 | 0.003354 | 0.004088 | 0.011521 | 0.049774 | 0.005979 | 0.003886 | 0.009550 | 0.048804 |
Cytoplasm | 0.042114 | 0.021338 | 0.009158 | 0.010157 | 0.044134 | 0.014250 | 0.008870 | 0.006437 | 0.043219 | 0.017350 | 0.008993 | 0.008570 |
Cytoplasmic Foci | 0.015573 | 0.009191 | 0.002751 | 0.001346 | 0.012308 | 0.016721 | 0.002555 | 0.001861 | 0.013786 | 0.013428 | 0.002639 | 0.001565 |
Eisosomes | 0.000073 | 0.000014 | 0.000013 | 0.000147 | 0.000059 | 0.000021 | 0.000031 | 0.000203 | 0.000065 | 0.000018 | 0.000023 | 0.000171 |
Endoplasmic Reticulum | 0.576279* | 0.493420* | 0.281497* | 0.721733* | 0.367883* | 0.550093* | 0.684108* | 0.742096* | 0.462244* | 0.525306* | 0.512384* | 0.730422* |
Endosome | 0.024705 | 0.047726 | 0.034627 | 0.013404 | 0.027749 | 0.064848 | 0.027844 | 0.012593 | 0.026371 | 0.057359 | 0.030737 | 0.013058 |
Golgi | 0.005469 | 0.009924 | 0.017039 | 0.009256 | 0.004390 | 0.015250 | 0.008941 | 0.015459 | 0.004879 | 0.012920 | 0.012395 | 0.011903 |
Lipid Particles | 0.012589 | 0.011522 | 0.019585 | 0.076569 | 0.005580 | 0.019538 | 0.027671 | 0.093823 | 0.008754 | 0.016032 | 0.024222 | 0.083932 |
Mitochondria | 0.000778 | 0.000534 | 0.000250 | 0.000933 | 0.000452 | 0.000838 | 0.000485 | 0.000589 | 0.000600 | 0.000705 | 0.000385 | 0.000786 |
Mitotic Spindle | 0.000041 | 0.000308 | 0.000302 | 0.000309 | 0.000195 | 0.000163 | 0.000077 | 0.000418 | 0.000125 | 0.000226 | 0.000173 | 0.000356 |
None | 0.000884 | 0.000294 | 0.000218 | 0.000749 | 0.008782 | 0.000351 | 0.000750 | 0.000527 | 0.005206 | 0.000326 | 0.000523 | 0.000654 |
Nuclear Periphery | 0.000421 | 0.002421 | 0.007134 | 0.002673 | 0.001898 | 0.001055 | 0.001463 | 0.002371 | 0.001229 | 0.001653 | 0.003881 | 0.002544 |
Nuclear Periphery Foci | 0.004129 | 0.022829 | 0.065920 | 0.009741 | 0.017061 | 0.011487 | 0.014808 | 0.006677 | 0.011206 | 0.016448 | 0.036608 | 0.008434 |
Nucleolus | 0.000139 | 0.000186 | 0.000059 | 0.000034 | 0.003279 | 0.000075 | 0.000035 | 0.000028 | 0.001857 | 0.000124 | 0.000045 | 0.000031 |
Nucleus | 0.000592 | 0.000896 | 0.000299 | 0.000421 | 0.007729 | 0.000274 | 0.000164 | 0.000371 | 0.004497 | 0.000546 | 0.000222 | 0.000399 |
Peroxisomes | 0.000632 | 0.000097 | 0.000026 | 0.000288 | 0.000317 | 0.000173 | 0.000195 | 0.000358 | 0.000460 | 0.000140 | 0.000123 | 0.000318 |
Vacuole | 0.281209* | 0.338985* | 0.505578* | 0.090529 | 0.442203* | 0.270170* | 0.198743 | 0.055866 | 0.369306* | 0.300267* | 0.329616* | 0.075738 |
Vacuole Periphery | 0.018553 | 0.032995 | 0.045316 | 0.005668 | 0.039341 | 0.026515 | 0.009240 | 0.003663 | 0.029928 | 0.029349 | 0.024627 | 0.004812 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.29 | 1.90 | -1.26 | -2.52 | -2.99 | -0.61 | -0.80 | -4.29 | -4.16 | -2.08 | 1.18 | 0.49 | -3.21 | -4.23 | -3.26 |
Bud Neck | 0.84 | -5.24 | -3.96 | -4.30 | -1.26 | 4.57 | 4.50 | 2.55 | -3.92 | -3.78 | 4.64 | 3.13 | 0.39 | -5.77 | -3.64 |
Bud Site | 2.74 | 3.81 | -0.73 | -3.20 | -4.09 | 4.85 | 6.25 | 2.25 | -2.20 | -3.61 | 5.54 | 7.29 | 1.45 | -3.71 | -5.42 |
Cell Periphery | 7.36 | 3.01 | -16.01 | -18.91 | -17.47 | -1.90 | -11.90 | -16.43 | -16.15 | -13.31 | 5.19 | -6.57 | -23.41 | -24.83 | -21.39 |
Cytoplasm | 6.39 | 12.07 | 11.23 | 4.63 | -0.67 | 9.84 | 12.34 | 12.76 | 4.56 | 1.85 | 11.70 | 17.17 | 16.61 | 5.99 | 0.27 |
Cytoplasmic Foci | 3.90 | 8.95 | 9.84 | 9.34 | 4.48 | -2.37 | 9.36 | 9.82 | 10.40 | 1.45 | 0.44 | 12.90 | 14.04 | 13.47 | 3.67 |
Eisosomes | 1.96 | 2.00 | -2.25 | -8.06 | -8.14 | 1.50 | 1.13 | -2.10 | -2.83 | -2.69 | 2.44 | 2.18 | -3.07 | -5.24 | -5.07 |
Endoplasmic Reticulum | 6.40 | 24.73 | -11.20 | -17.79 | -38.40* | -16.07 | -29.84 | -31.75 | -15.51 | -5.26 | -7.07 | -5.22 | -30.36 | -22.99 | -25.11 |
Endosome | -10.28 | -6.29 | 8.10 | 16.48 | 15.94 | -14.48 | -0.27 | 10.02 | 22.65 | 13.48 | -17.75 | -4.03 | 12.70 | 27.75 | 20.29 |
Golgi | -3.83 | -9.81 | -4.69 | 0.50 | 6.06 | -8.28 | -5.83 | -8.48 | -0.12 | -4.83 | -8.97 | -11.10 | -9.64 | 0.95 | 0.45 |
Lipid Particles | 0.59 | -4.07 | -15.65 | -15.81 | -13.76 | -8.25 | -11.94 | -17.21 | -13.94 | -12.28 | -5.98 | -11.86 | -23.55 | -20.60 | -17.90 |
Mitochondria | 1.37 | 5.03 | -0.59 | -1.30 | -2.40 | -1.62 | -0.39 | -1.58 | 0.75 | -1.14 | -0.68 | 3.12 | -1.24 | -0.52 | -2.43 |
Mitotic Spindle | -2.00 | -3.21 | -5.37 | -0.05 | -0.07 | 0.41 | 1.82 | -1.53 | -1.64 | -1.92 | -1.43 | -1.09 | -2.48 | -1.62 | -2.08 |
None | 1.15 | 1.31 | 0.17 | -2.50 | -2.97 | 5.42 | 5.15 | 5.28 | -1.42 | 0.76 | 5.52 | 5.27 | 5.07 | -2.74 | -1.09 |
Nuclear Periphery | -7.64 | -15.28 | -18.59 | -0.90 | 9.94 | 2.68 | 1.25 | -1.40 | -8.11 | -5.48 | -2.06 | -10.50 | -6.96 | -5.88 | 6.48 |
Nuclear Periphery Foci | -10.44 | -20.43 | -6.33 | 6.44 | 17.88 | 3.16 | 0.92 | 5.94 | 4.69 | 6.64 | -3.96 | -14.67 | 2.35 | 7.26 | 17.70 |
Nucleolus | -0.45 | 4.41 | 5.73 | 1.43 | 5.81 | 6.73 | 6.82 | 6.83 | 6.80 | 1.36 | 6.52 | 6.91 | 6.96 | 1.95 | 4.31 |
Nucleus | -0.79 | 2.13 | 0.58 | 1.03 | -1.62 | 6.30 | 6.39 | 6.01 | -1.93 | -2.63 | 5.85 | 6.52 | 6.04 | 0.13 | -3.30 |
Peroxisomes | 6.61 | 7.83 | 4.04 | -4.20 | -6.84 | 3.47 | 2.86 | -0.34 | -2.27 | -2.00 | 7.39 | 7.85 | 2.61 | -4.12 | -4.59 |
Vacuole | -5.07 | -20.47 | 20.41 | 26.43 | 47.55* | 17.29 | 25.94 | 47.49* | 28.26 | 21.90 | 8.89 | 4.80 | 46.05* | 37.08 | 42.77* |
Vacuole Periphery | -7.14 | -12.75 | 10.34 | 15.22 | 21.37* | 6.40 | 18.12 | 21.92 | 17.81 | 9.18 | 0.42 | 4.27 | 23.22 | 22.69 | 21.33* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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