Standard name
Human Ortholog
Description Mitochondrial translation initiation factor 2
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0.07 0.07 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0.07 0.05 – 0.16 0.05 0 0 0.12 0 0.06 0.06 0.2 0.2 0.21 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.08 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0.05 – – – 0 0 0 0 0 0
Endosome 0 0.07 0.12 – 0.16 0 0.06 0.05 0.08 0 0 0 0.18 0.1 0.23 – – – 0.07 0.07 0.12 0.07 0.07 0.05
Golgi 0 0 0.05 – 0.11 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0.08 0 0.06 0.06 0.06
Mitochondria 0.88 0.85 0.62 – 0.53 0.86 0.7 0.78 0.73 0.85 0.89 0.94 0.44 0.31 0.34 – – – 0.73 0.7 0.6 0.68 0.66 0.67
Nucleus 0 0 0 – 0 0 0 0.06 0.07 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0.07 0.14 – 0.11 0 0 0 0 0.05 0 0 0.08 0.31 0.2 – – – 0.07 0 0 0 0 0
SpindlePole 0 0 0 – 0.05 0.05 0.09 0.09 0.05 0.08 0 0.06 0 0.07 0.05 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.1 0.17 – 0.11 0.09 0.1 0.06 0.08 0.06 0 0 0.24 0.11 0.08 – – – 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 – 0 0 0 0 0 0 0 0 1 6 0 – – – 1 2 2 0 3 9
Bud 4 4 1 – 0 0 1 5 2 6 6 0 2 2 0 – – – 0 0 0 1 1 2
Bud Neck 0 0 0 – 0 0 0 0 1 0 0 0 0 1 0 – – – 0 0 1 0 0 1
Bud Site 2 0 0 – 0 0 1 0 0 1 0 0 0 0 0 – – – – – – – – –
Cell Periphery 5 4 1 – 0 1 1 0 1 0 0 0 1 8 3 – – – 0 0 0 0 0 1
Cytoplasm 3 11 2 – 3 3 3 6 7 2 5 3 23 28 23 – – – 0 0 2 3 2 5
Endoplasmic Reticulum 16 2 0 – 0 0 3 4 2 2 2 3 0 2 5 – – – 0 0 0 0 0 2
Endosome 4 11 5 – 3 2 4 8 5 3 0 0 21 14 25 – – – 16 12 9 7 11 10
Golgi 5 7 2 – 2 0 1 1 0 0 0 1 6 4 4 – – – 8 13 2 6 9 13
Mitochondria 181 135 26 – 10 50 47 116 44 71 71 46 51 44 37 – – – 164 120 51 67 106 148
Nucleus 1 1 0 – 0 0 3 9 4 0 1 0 0 2 0 – – – 0 0 0 0 0 2
Nuclear Periphery 6 1 0 – 0 0 1 2 1 1 2 3 0 0 1 – – – 2 1 1 0 1 1
Nucleolus 2 2 0 – 1 3 3 1 0 2 2 2 1 0 0 – – – 0 0 1 0 1 0
Peroxisomes 3 11 6 – 2 1 1 3 0 4 0 0 9 44 22 – – – 15 7 3 3 6 5
SpindlePole 3 2 1 – 1 3 6 14 3 7 3 3 2 10 5 – – – 2 1 1 2 2 1
Vac/Vac Membrane 28 16 7 – 2 5 7 9 5 5 2 0 28 15 9 – – – 7 5 3 3 10 13
Unique Cell Count 206 158 42 19 58 67 149 60 84 80 49 117 142 109 224 172 85 99 161 222
Labelled Cell Count 265 207 53 24 68 82 178 75 104 94 61 145 180 134 224 172 85 99 161 222


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.6 6.1 – 7.5 5.3 5.4 5.8 5.4 4.8 4.9 4.3 7.5 7.4 6.7 – – – 8.1 6.8 7.4
Std Deviation (1e-4) 0.7 1.2 1.3 – 2.0 1.5 1.2 1.5 1.5 1.1 1.6 0.9 3.3 3.6 2.4 – – – 4.5 2.2 2.9
Intensity Change (Log2) – – – – 0.29 -0.22 -0.17 -0.07 -0.18 -0.35 -0.31 -0.5 0.3 0.27 0.14 – – – 0.41 0.15 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – 0 0 0 0 0 – 0 – 0 0 0 – – –
Bud Neck – 0 0 0 0 0 – 0 – 0 0 0 – – –
Bud Site – 0 0 0 0 0 – 0 – 0 0 0 – – –
Cell Periphery – 0 0 0 0 0 – 0 – 0 0 0 – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 0 0 0 0 – 0 – 0 0 0 – – –
Endosome – 0 0 0 0 0 – 0 – 0.9 0 1.5 – – –
Golgi – 0 0 0 0 0 – 0 – 0 0 0 – – –
Mitochondria – -0.7 2.8 0.9 2.1 1.2 – 3.5 – -2.0 -3.6 -3.1 – – –
Nuclear Periphery – 0 0 0 0 0 – 0 – 0 0 0 – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – 0 0 0 0 0 – 0 – 0 0 0 – – –
Peroxisomes – 0 0 0 0 0 – 0 – 0 2.1 0.8 – – –
SpindlePole – 0 0 0 0 0 – 0 – 0 0 0 – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – 0 0 0 0 0 – 0 – 0 0 0 – – –
Cytoplasm – 0 0 0 0 0 – 0 – 2.3 2.3 2.4 – – –
Nucleus – 0 0 0 0 0 – 0 – 0 0 0 – – –
Vacuole – 0 -1.2 -0.9 0 0 – 0 – 1.0 -1.1 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.947 0.1971 0.4112 0.7073 0.1733 0.36 0.1002 0.7308 0.7167 0.7722 -0.3326 0.5379 0.6552 0.9145 0.7853 1.2879 1.215 0.6135
Actin 0.0269 0.0374 0.0375 0.0311 0.0072 0.0188 0.0521 0.0201 0.0351 0.012 0.0046 0.0299 0.0191 0.0146 0.0276 0.0515 0.0525 0.0076
Bud 0.0011 0.0031 0.0069 0.0021 0.0013 0.0009 0.0014 0.0076 0.0019 0.0002 0.0006 0.01 0.0011 0.0036 0.0012 0.0072 0.004 0.0006
Bud Neck 0.0016 0.0045 0.0077 0.004 0.0006 0.018 0.0049 0.0012 0.004 0.0002 0.0038 0.0262 0.001 0.0021 0.0018 0.0014 0.0028 0.0015
Bud Periphery 0.0014 0.0058 0.0126 0.0042 0.0028 0.0022 0.0027 0.0137 0.0042 0.0004 0.0013 0.0109 0.0059 0.0047 0.0016 0.0056 0.0088 0.0009
Bud Site 0.0074 0.0332 0.0389 0.0032 0.0025 0.0012 0.0155 0.0293 0.022 0.0008 0.0017 0.0039 0.0048 0.0361 0.007 0.0028 0.0086 0.0011
Cell Periphery 0.0005 0.0018 0.0014 0.0005 0.0002 0.0007 0.0008 0.0009 0.0035 0.0002 0.0003 0.0012 0.0059 0.0018 0.0009 0.0003 0.0007 0.0002
Cytoplasm 0.0054 0.0226 0.0072 0.0047 0.0014 0.0226 0.0397 0.02 0.0125 0.0003 0.0021 0.0162 0.0046 0.0268 0.0126 0.0062 0.0204 0.0071
Cytoplasmic Foci 0.1294 0.1034 0.0911 0.058 0.0352 0.0774 0.0775 0.099 0.0469 0.0224 0.0342 0.0616 0.0735 0.099 0.0548 0.0678 0.1029 0.0411
Eisosomes 0.001 0.0019 0.0006 0.0005 0.0002 0.0005 0.0009 0.0005 0.0009 0.0002 0.0001 0.0005 0.0014 0.0007 0.0011 0.0004 0.0003 0.0002
Endoplasmic Reticulum 0.0041 0.0046 0.0012 0.0016 0.0004 0.0042 0.0023 0.0029 0.0011 0 0.0001 0.0012 0.0036 0.0039 0.0018 0.001 0.0039 0.001
Endosome 0.2983 0.1385 0.0817 0.1348 0.0985 0.1419 0.0881 0.0732 0.0561 0.0092 0.0252 0.0443 0.1226 0.1536 0.1106 0.0957 0.1211 0.0946
Golgi 0.1301 0.0751 0.0642 0.076 0.1173 0.0957 0.0533 0.0552 0.0407 0.0117 0.0209 0.0354 0.0836 0.1064 0.0955 0.0888 0.1112 0.0881
Lipid Particles 0.0856 0.0387 0.0571 0.0445 0.0119 0.043 0.0607 0.0253 0.0168 0.0036 0.0028 0.0452 0.0629 0.0237 0.018 0.0043 0.0098 0.0152
Mitochondria 0.1747 0.3894 0.4906 0.5643 0.6187 0.4544 0.3889 0.4692 0.5949 0.8116 0.7073 0.5321 0.5156 0.4197 0.5959 0.6034 0.4249 0.6685
None 0.0151 0.0165 0.035 0.0021 0.0004 0.0326 0.0357 0.0612 0.0326 0.0002 0.0003 0.0466 0.0079 0.0143 0.0182 0.0111 0.0013 0.0006
Nuclear Periphery 0.0118 0.0023 0.0006 0.001 0.0001 0.002 0.0069 0.0013 0.0016 0 0 0.0012 0.0062 0.001 0.0008 0.0008 0.0066 0.0059
Nucleolus 0.0019 0.0048 0.0014 0.0005 0.0002 0.0006 0.001 0.002 0.0015 0.0001 0.0002 0.0005 0.0006 0.0008 0.0004 0.0018 0.0003 0.0001
Nucleus 0.0016 0.0011 0.0008 0.0007 0.0003 0.0016 0.0019 0.0031 0.0009 0.0001 0.0002 0.0008 0.0015 0.0009 0.0007 0.0017 0.001 0.0003
Peroxisomes 0.0668 0.0887 0.0505 0.0497 0.0945 0.0724 0.1419 0.1023 0.1075 0.1256 0.1909 0.1142 0.0651 0.0669 0.035 0.0402 0.0826 0.0568
Punctate Nuclear 0.0219 0.0005 0.001 0.0016 0.0002 0.0016 0.0097 0.003 0.0008 0.0002 0.0001 0.0008 0.0006 0.0008 0.0008 0.0016 0.0064 0.0002
Vacuole 0.008 0.0193 0.007 0.0074 0.0029 0.0046 0.0094 0.0066 0.0097 0.0005 0.0025 0.0061 0.0069 0.0134 0.0084 0.0031 0.008 0.0034
Vacuole Periphery 0.0055 0.0066 0.005 0.0074 0.0031 0.0032 0.0047 0.0024 0.0049 0.0005 0.0008 0.0113 0.0056 0.0052 0.0054 0.0031 0.0219 0.0048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9761 8.2562 5.7661 7.8094 7.3961 10.8596 9.6983 11.1028 8.2176 10.8313
Translational Efficiency 0.7516 0.6048 0.6073 0.5412 0.4758 0.4545 0.7202 0.5025 0.7444 0.6848

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
868 1596 281 1504 1873 2607 140 272 2741 4203 421 1776

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 602.54 690.53 941.48 893.13 681.51 685.07 1036.97 931.53 656.50 687.14 973.23 899.01
Standard Deviation 66.67 86.85 146.65 129.77 74.54 86.35 126.53 146.02 80.96 86.58 147.32 133.11
Intensity Change Log 2 — 0.196647 0.643873 0.567813 — 0.007517 0.605567 0.450867 — 0.099360 0.623669 0.506926

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000242 0.000659 0.000589 0.000076 0.000274 0.000686 0.000857 0.000073 0.000262 0.000668 0.000630
Bud Neck 0.017402 0.033396 0.004223 0.016488 0.009886 0.040137 0.001833 0.017803 0.012266 0.037577 0.003428 0.016690
Bud Site 0.002207 0.010209 0.000781 0.018574 0.002048 0.013688 0.000579 0.054949 0.002098 0.012367 0.000714 0.024145
Cell Periphery 0.000228 0.000109 0.000086 0.000249 0.000153 0.000161 0.000092 0.000158 0.000177 0.000141 0.000088 0.000235
Cytoplasm 0.493480* 0.410345* 0.010944 0.161289* 0.402308* 0.400810* 0.017123 0.181253* 0.431180* 0.404431* 0.012999 0.164347*
Cytoplasmic Foci 0.186414* 0.196626* 0.002022 0.003368 0.146300 0.181826* 0.002156 0.003385 0.159003 0.187446* 0.002067 0.003370
Eisosomes 0.000238 0.000105 0.000044 0.000030 0.000110 0.000119 0.000055 0.000043 0.000151 0.000114 0.000047 0.000032
Endoplasmic Reticulum 0.000334 0.000593 0.006640 0.005871 0.000575 0.000802 0.007264 0.004872 0.000499 0.000723 0.006847 0.005718
Endosome 0.010437 0.017928 0.000521 0.001946 0.005017 0.017262 0.000691 0.002384 0.006734 0.017515 0.000578 0.002013
Golgi 0.001077 0.002692 0.000054 0.002483 0.001280 0.003249 0.000054 0.000249 0.001216 0.003037 0.000054 0.002141
Lipid Particles 0.008838 0.007573 0.000593 0.000603 0.008019 0.008264 0.000855 0.001440 0.008278 0.008001 0.000680 0.000731
Mitochondria 0.002068 0.013238 0.000623 0.003659 0.006259 0.014887 0.000592 0.003590 0.004932 0.014261 0.000613 0.003648
Mitotic Spindle 0.000037 0.005413 0.003410 0.016618 0.005596 0.008830 0.000263 0.030743 0.003836 0.007532 0.002363 0.018781
None 0.009852 0.004536 0.003576 0.006646 0.005206 0.004874 0.002947 0.011479 0.006677 0.004746 0.003367 0.007386
Nuclear Periphery 0.000527 0.000612 0.000534 0.002502 0.000723 0.000707 0.000642 0.002026 0.000661 0.000671 0.000570 0.002429
Nuclear Periphery Foci 0.000230 0.000415 0.000225 0.000826 0.000243 0.000244 0.000264 0.000913 0.000239 0.000309 0.000238 0.000839
Nucleolus 0.001274 0.002603 0.004972 0.001024 0.001523 0.002777 0.003213 0.001400 0.001444 0.002711 0.004387 0.001082
Nucleus 0.221476* 0.211756* 0.953837* 0.726426* 0.377743* 0.217637* 0.946766* 0.624898* 0.328257* 0.215404* 0.951485* 0.710876*
Peroxisomes 0.003309 0.014433 0.000332 0.000289 0.004800 0.012292 0.000259 0.000912 0.004328 0.013105 0.000308 0.000385
Vacuole 0.039931 0.065230 0.005811 0.029941 0.021594 0.069000 0.013549 0.056107 0.027401 0.067568 0.008384 0.033948
Vacuole Periphery 0.000575 0.001947 0.000113 0.000579 0.000540 0.002159 0.000117 0.000539 0.000551 0.002078 0.000114 0.000573

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.84 -11.08 -12.30 -8.57 0.40 -3.36 -8.92 -8.34 -6.60 -2.28 -4.97 -14.24 -14.85 -9.20 -0.10
Bud Neck -5.27 5.97 0.02 6.31 -9.67 -14.57 6.55 -3.76 7.27 -6.89 -14.98 8.14 -3.95 11.33 -11.95
Bud Site -7.47 3.15 -6.01 -1.18 -6.82 -9.30 2.83 -3.40 -1.25 -3.59 -11.68 4.10 -6.98 -0.72 -7.69
Cell Periphery 2.44 2.58 0.85 -4.61 -4.27 -0.50 2.40 0.03 0.46 -2.20 2.10 3.86 -0.12 -2.71 -4.65
Cytoplasm 8.06 55.80 38.74 32.28 -19.71 0.22 47.70 22.01 22.65 -8.38 4.26 73.19 45.04 44.57 -21.13
Cytoplasmic Foci -1.43 31.21 31.38 37.61 -0.49 -5.91 32.03 32.55 44.36 -0.12 -5.99 43.69 44.16 58.34 -0.39
Eisosomes 7.19 10.44 11.11 9.75 1.16 -1.04 4.32 4.02 5.00 -0.77 4.78 12.00 14.74 14.75 1.70
Endoplasmic Reticulum -4.78 -13.91 -23.03 -22.28 -1.18 -1.93 -11.03 -10.94 -10.68 -1.83 -3.07 -17.49 -25.04 -24.02 -1.26
Endosome -4.54 8.17 7.37 13.54 -3.87 -12.26 9.34 6.14 15.57 -3.62 -12.56 12.69 10.48 21.11 -4.41
Golgi -3.99 3.78 0.12 2.64 -1.70 -4.48 6.65 5.83 7.66 -3.71 -5.82 7.67 0.71 3.97 -1.74
Lipid Particles 1.02 8.38 8.44 9.97 -0.22 -0.24 12.17 10.97 9.88 -1.73 0.42 15.22 15.53 15.12 -0.38
Mitochondria -7.34 4.06 -0.92 6.76 -3.88 -5.35 4.93 3.23 10.25 -3.84 -7.73 5.44 2.66 11.66 -4.43
Mitotic Spindle -4.32 -1.04 -8.02 -5.32 -4.09 -1.61 3.40 -3.73 -3.33 -4.47 -2.59 0.64 -6.98 -5.63 -6.14
None 3.25 2.90 2.53 -2.02 -1.47 0.37 2.06 -3.35 -3.52 -4.16 2.71 2.77 0.16 -3.27 -2.85
Nuclear Periphery -0.93 -0.92 -17.07 -20.31 -19.28 0.26 -1.37 -10.96 -11.01 -9.86 -0.24 -0.85 -21.83 -22.42 -21.09
Nuclear Periphery Foci -2.42 -1.31 -3.49 -2.73 -2.95 0.78 -1.09 -3.89 -4.40 -3.28 -0.03 -0.90 -3.80 -3.96 -3.46
Nucleolus -5.70 -3.89 -1.01 3.93 3.52 -3.84 -2.67 -1.34 0.63 1.47 -5.81 -4.38 -0.48 4.41 4.10
Nucleus 0.88 -78.91* -50.55* -54.61* 22.49 18.87 -40.22 -13.62 -22.64 13.06 16.98 -77.80 -44.30 -64.36 24.95
Peroxisomes -9.75 7.63 8.02 13.22 2.72 -8.49 9.76 8.51 15.26 -1.89 -12.42 11.75 11.89 20.75 1.24
Vacuole -7.53 10.03 -5.70 0.33 -13.67 -18.64 -0.53 -8.05 -4.08 -6.50 -19.90 4.26 -12.03 -1.41 -12.72
Vacuole Periphery -7.55 7.00 1.57 7.54 -1.88 -7.94 4.64 2.19 9.59 -3.18 -10.46 6.70 1.65 10.26 -2.12
Standard name
Human Ortholog
Description Mitochondrial translation initiation factor 2
Localization
Cell Percentages cytoplasm (17%), nucleus (10%), mixed (38%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00042)
Subcompartmental Group N/A

Ifm1

Ifm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ifm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available