Standard name
Description High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.08 0.17 0.14 0 0 0 0 – 0 0 0 0.15 0.14 0.06 0 0 0 0 0 0 0 0 0
Bud 0.05 0.1 0.05 0.08 0 0 0.05 0 – 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.65 0.64 0.55 0.56 0.18 0.15 0.09 0.08 – 0.07 0.09 0.08 0.86 0.86 0.8 0.11 0.05 0.13 0.23 0.32 0.33 0.57 0.71 0.66
Cytoplasm 0.46 0.5 0.35 0.58 0.57 0.73 0.72 0.75 – 0.83 0.84 0.84 0.21 0.21 0.2 0.73 0.8 0.77 0.17 0.15 0 0 0 0
Endoplasmic Reticulum 0.11 0.06 0.15 0.07 0.24 0.09 0 0 – 0 0 0 0 0 0 0 0 0 0.48 0.43 0.44 0.27 0.16 0.22
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 – 0.11 0.11 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.19 0.07 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.13 0.23 0.18 – 0.11 0.09 0.09 0 0 0 0.07 0.1 0.08 0.08 0 0.12 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 16 31 29 5 5 1 7 – 1 5 4 32 30 12 1 1 2 3 5 4 7 12 7
Bud 13 20 9 16 5 7 15 15 – 14 29 16 8 8 7 1 0 1 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 6 6 3 3 3 1
Bud Site 0 0 0 0 0 1 0 0 – 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 166 129 99 114 45 43 27 31 – 23 29 35 179 179 163 14 2 12 66 72 66 161 289 240
Cytoplasm 117 102 63 119 140 207 229 294 – 273 281 372 43 43 42 90 33 70 49 32 8 11 9 7
Endoplasmic Reticulum 28 13 27 14 60 25 13 10 – 9 1 7 7 6 8 3 0 0 141 96 88 78 67 80
Endosome 0 0 0 0 5 0 1 0 – 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0
Golgi 1 0 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 1 1 2 5 11 20 – 37 36 37 0 0 0 1 2 1 0 0 3 5 6 3
Nucleus 0 0 0 0 9 8 4 11 – 3 2 3 1 1 3 24 3 7 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 3 3 – 2 1 6 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 2 2 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 25 38 73 71 – 37 29 38 0 0 0 9 4 7 24 8 23 12 10 13
Unique Cell Count 257 202 179 205 245 285 316 390 329 333 444 209 207 205 124 41 91 293 226 200 285 408 363
Labelled Cell Count 343 280 233 294 296 339 377 462 399 417 522 270 267 235 145 45 101 293 226 200 285 408 363


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 16.2 13.4 12.9 10.9 8.5 7.1 6.8 – 5.3 5.4 5.3 14.4 14.4 13.7 9.2 9.0 12.6 17.3 16.8 17.0
Std Deviation (1e-4) 2.6 2.9 2.3 2.4 2.2 2.5 2.4 2.3 – 2.1 2.0 1.9 2.5 2.5 2.2 1.6 2.2 2.7 3.1 2.7 2.9
Intensity Change (Log2) – – – -0.06 -0.3 -0.65 -0.92 -0.98 – -1.34 -1.31 -1.33 0.11 0.11 0.03 -0.54 -0.58 -0.09 0.37 0.33 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -5.6 -6.1 -7.4 -6.9 – -7.5 -6.7 -8.1 -0.5 -0.8 -3.6 -4.6 -2.4 -3.6
Bud 1.1 -1.7 -1.5 -0.1 -0.7 – -0.4 1.5 -0.8 -0.6 -0.6 -0.8 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -7.9 -9.2 -11.5 -12.5 – -12.2 -11.6 -13.0 6.6 6.8 5.1 -7.8 -5.8 -6.6
Cytoplasm 4.5 4.5 8.0 8.1 9.2 – 10.9 11.3 12.0 -3.2 -3.2 -3.2 6.4 5.3 6.5
Endoplasmic Reticulum -2.6 2.4 -2.1 -4.3 -5.6 – -5.2 -7.0 -6.7 -4.1 -4.3 -3.8 -3.6 -2.7 -3.9
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.7 – 4.4 4.3 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 6.1 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 4.1 4.8 6.8 5.9 – 4.4 3.7 3.7 0 0 0 0 0 0