Standard name | |
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Human Ortholog | |
Description | High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 16 | 31 | 29 | 5 | 5 | 1 | 7 | – | 1 | 5 | 4 | 32 | 30 | 12 | 1 | 1 | 2 | 3 | 5 | 4 | 7 | 12 | 7 |
Bud | 13 | 20 | 9 | 16 | 5 | 7 | 15 | 15 | – | 14 | 29 | 16 | 8 | 8 | 7 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 3 | 3 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 166 | 129 | 99 | 114 | 45 | 43 | 27 | 31 | – | 23 | 29 | 35 | 179 | 179 | 163 | 14 | 2 | 12 | 66 | 72 | 66 | 161 | 289 | 240 |
Cytoplasm | 117 | 102 | 63 | 119 | 140 | 207 | 229 | 294 | – | 273 | 281 | 372 | 43 | 43 | 42 | 90 | 33 | 70 | 49 | 32 | 8 | 11 | 9 | 7 |
Endoplasmic Reticulum | 28 | 13 | 27 | 14 | 60 | 25 | 13 | 10 | – | 9 | 1 | 7 | 7 | 6 | 8 | 3 | 0 | 0 | 141 | 96 | 88 | 78 | 67 | 80 |
Endosome | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 1 | 1 | 2 | 5 | 11 | 20 | – | 37 | 36 | 37 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 5 | 6 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 9 | 8 | 4 | 11 | – | 3 | 2 | 3 | 1 | 1 | 3 | 24 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | – | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 25 | 38 | 73 | 71 | – | 37 | 29 | 38 | 0 | 0 | 0 | 9 | 4 | 7 | 24 | 8 | 23 | 12 | 10 | 13 |
Unique Cell Count | 257 | 202 | 179 | 205 | 245 | 285 | 316 | 390 | 329 | 333 | 444 | 209 | 207 | 205 | 124 | 41 | 91 | 293 | 226 | 200 | 285 | 408 | 363 | |
Labelled Cell Count | 343 | 280 | 233 | 294 | 296 | 339 | 377 | 462 | 399 | 417 | 522 | 270 | 267 | 235 | 145 | 45 | 101 | 293 | 226 | 200 | 285 | 408 | 363 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.6 | 16.2 | 13.4 | 12.9 | 10.9 | 8.5 | 7.1 | 6.8 | – | 5.3 | 5.4 | 5.3 | 14.4 | 14.4 | 13.7 | 9.2 | 9.0 | 12.6 | 17.3 | 16.8 | 17.0 |
Std Deviation (1e-4) | 2.6 | 2.9 | 2.3 | 2.4 | 2.2 | 2.5 | 2.4 | 2.3 | – | 2.1 | 2.0 | 1.9 | 2.5 | 2.5 | 2.2 | 1.6 | 2.2 | 2.7 | 3.1 | 2.7 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.3 | -0.65 | -0.92 | -0.98 | – | -1.34 | -1.31 | -1.33 | 0.11 | 0.11 | 0.03 | -0.54 | -0.58 | -0.09 | 0.37 | 0.33 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -5.6 | -6.1 | -7.4 | -6.9 | – | -7.5 | -6.7 | -8.1 | -0.5 | -0.8 | -3.6 | -4.6 | -2.4 | -3.6 |
Bud | 1.1 | -1.7 | -1.5 | -0.1 | -0.7 | – | -0.4 | 1.5 | -0.8 | -0.6 | -0.6 | -0.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -7.9 | -9.2 | -11.5 | -12.5 | – | -12.2 | -11.6 | -13.0 | 6.6 | 6.8 | 5.1 | -7.8 | -5.8 | -6.6 |
Cytoplasm | 4.5 | 4.5 | 8.0 | 8.1 | 9.2 | – | 10.9 | 11.3 | 12.0 | -3.2 | -3.2 | -3.2 | 6.4 | 5.3 | 6.5 |
Endoplasmic Reticulum | -2.6 | 2.4 | -2.1 | -4.3 | -5.6 | – | -5.2 | -7.0 | -6.7 | -4.1 | -4.3 | -3.8 | -3.6 | -2.7 | -3.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.7 | – | 4.4 | 4.3 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.1 | 4.8 | 6.8 | 5.9 | – | 4.4 | 3.7 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.4822 | 21.5009 | 19.2671 | 16.8984 | 15.1623 | 19.1814 | 16.7519 | 24.7564 | 20.0802 | 19.9436 | 19.8145 | 20.5397 | 19.2909 | 26.541 | 22.4238 | 21.2746 | 20.6965 | 22.5118 |
Actin | 0.019 | 0.0004 | 0.0004 | 0.0118 | 0.0155 | 0.0003 | 0.027 | 0.0003 | 0.0142 | 0.0094 | 0.0065 | 0.0011 | 0.0197 | 0.0001 | 0.0051 | 0.0023 | 0.0151 | 0.0095 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0036 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0005 |
Bud Neck | 0.0013 | 0.0009 | 0.0025 | 0.0042 | 0.0022 | 0.0021 | 0.0012 | 0.0013 | 0.0021 | 0.0043 | 0.0015 | 0.0026 | 0.0008 | 0.0008 | 0.0031 | 0.0035 | 0.0108 | 0.0064 |
Bud Periphery | 0.0026 | 0.0008 | 0.0025 | 0.0254 | 0.002 | 0.003 | 0.0028 | 0.0013 | 0.0071 | 0.0039 | 0.0039 | 0.0029 | 0.0042 | 0.0014 | 0.0041 | 0.0082 | 0.0183 | 0.008 |
Bud Site | 0.0017 | 0.0027 | 0.0009 | 0.0045 | 0.0028 | 0.0003 | 0.0041 | 0.0024 | 0.0167 | 0.0006 | 0.001 | 0.0002 | 0.0015 | 0.0016 | 0.0022 | 0.0003 | 0.0018 | 0.001 |
Cell Periphery | 0.5332 | 0.5352 | 0.5126 | 0.4255 | 0.3909 | 0.5575 | 0.4624 | 0.6372 | 0.5298 | 0.5089 | 0.5162 | 0.5405 | 0.5721 | 0.6891 | 0.6613 | 0.6492 | 0.6503 | 0.6633 |
Cytoplasm | 0.0548 | 0.1047 | 0.0983 | 0.1483 | 0.0438 | 0.0786 | 0.0175 | 0.0438 | 0.018 | 0.0214 | 0.0315 | 0.0265 | 0.0389 | 0.0641 | 0.0264 | 0.0327 | 0.047 | 0.0236 |
Cytoplasmic Foci | 0.0054 | 0.0004 | 0.0004 | 0.0024 | 0.0066 | 0.0004 | 0.0066 | 0.0003 | 0.0064 | 0.0069 | 0.0064 | 0.002 | 0.0036 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0003 |
Eisosomes | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0001 | 0.0111 | 0.0004 | 0.0054 | 0.016 | 0.0005 | 0.0003 | 0.0094 | 0.0051 | 0.017 | 0.0015 | 0.0034 | 0.0066 |
Endoplasmic Reticulum | 0.1395 | 0.1827 | 0.2063 | 0.102 | 0.2729 | 0.1796 | 0.2229 | 0.1898 | 0.2234 | 0.2337 | 0.205 | 0.2307 | 0.2285 | 0.1588 | 0.1937 | 0.2107 | 0.122 | 0.1752 |
Endosome | 0.0117 | 0.001 | 0.001 | 0.0258 | 0.0119 | 0.0015 | 0.0212 | 0.0001 | 0.0031 | 0.0065 | 0.035 | 0.0037 | 0.0021 | 0 | 0.0006 | 0.0003 | 0.0006 | 0.0003 |
Golgi | 0.0031 | 0.0006 | 0.0003 | 0.0026 | 0.0062 | 0.0006 | 0.0101 | 0.0003 | 0.0045 | 0.0049 | 0.0077 | 0.001 | 0.0025 | 0.0002 | 0.0016 | 0.0008 | 0.0022 | 0.0008 |
Lipid Particles | 0.0434 | 0.0667 | 0.0459 | 0.0405 | 0.07 | 0.035 | 0.0475 | 0.0305 | 0.0442 | 0.0444 | 0.0397 | 0.0513 | 0.0185 | 0.0149 | 0.0118 | 0.0086 | 0.0164 | 0.0208 |
Mitochondria | 0.0056 | 0.0002 | 0.0003 | 0.0042 | 0.005 | 0.0001 | 0.0183 | 0 | 0.008 | 0.001 | 0.0028 | 0.0003 | 0.0016 | 0 | 0.0007 | 0 | 0.0312 | 0.0061 |
None | 0.0085 | 0.0002 | 0.0028 | 0.0023 | 0.0024 | 0.0001 | 0.0056 | 0.0001 | 0.0015 | 0.0003 | 0.0075 | 0.0002 | 0.0013 | 0.0002 | 0.0034 | 0.0001 | 0.0004 | 0.0009 |
Nuclear Periphery | 0.0143 | 0.0062 | 0.0069 | 0.0259 | 0.0123 | 0.0054 | 0.025 | 0.0129 | 0.0116 | 0.0091 | 0.0193 | 0.0063 | 0.015 | 0.0107 | 0.0121 | 0.0083 | 0.0079 | 0.0067 |
Nucleolus | 0.0009 | 0.0002 | 0.0004 | 0.0011 | 0.0145 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0002 |
Nucleus | 0.0034 | 0.0003 | 0.0009 | 0.0074 | 0.0044 | 0.0003 | 0.0012 | 0.0003 | 0.0006 | 0.0003 | 0.0041 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0012 | 0.0004 |
Peroxisomes | 0.0034 | 0 | 0 | 0.0006 | 0.0269 | 0 | 0.0026 | 0 | 0.0162 | 0.0037 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0039 | 0 | 0.0001 | 0.0038 | 0.0012 | 0 | 0.0055 | 0 | 0.0007 | 0.0006 | 0.0067 | 0.0006 | 0.006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Vacuole | 0.1423 | 0.0963 | 0.1163 | 0.1548 | 0.1021 | 0.1344 | 0.1048 | 0.0787 | 0.0838 | 0.1233 | 0.1016 | 0.1288 | 0.0723 | 0.0523 | 0.0551 | 0.0719 | 0.0658 | 0.0689 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0005 | 0.003 | 0.005 | 0.0003 | 0.0017 | 0.0002 | 0.0008 | 0.0004 | 0.0016 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0005 | 0.0026 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.8596 | 47.6832 | 53.8973 | 53.0204 | 54.2812 | 48.3518 | 57.7428 | 52.5939 | 60.8324 | 65.1256 |
Translational Efficiency | 0.7881 | 0.848 | 0.6843 | 0.7681 | 0.7191 | 0.823 | 0.6577 | 0.734 | 0.6564 | 0.5586 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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