Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.05 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0.08 0 0 – 0 0 0 0 0 0.05 0.06 0.25 0.19 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0 0
Endosome 0 0 – 0.07 0.12 0 0.1 – 0 0 0 0 0.06 0.05 0 0.15 0.19 0.09 0.05 0.06 0.08 0.05 0.07 0.06
Golgi 0 0 – 0.18 0.2 0 0 – 0 0 0 0 0.19 0.14 0.1 0.13 0.14 0.23 0.05 0.07 0 0.05 0.07 0.09
Mitochondria 0.9 0.88 – 0.79 0.58 0.87 0.73 – 0.89 0.85 0.92 0.86 0.8 0.86 0.81 0.12 0.16 0.06 0.8 0.73 0.66 0.72 0.64 0.63
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 – 0 0 0 0.07 – 0.13 0.06 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 – 0 0 0 0.07 – 0.08 0.08 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0.09 0.05 0 0.11 0.09 0.1 0.09
SpindlePole 0 0 – 0 0 0 0.07 – 0 0 0 0 0 0 0 0.07 0.16 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.14 – 0.08 0.28 0.07 0.16 – 0.05 0 0 0 0.15 0.15 0.11 0.17 0.27 0.34 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 – 5 0 1 0 – 0 0 2 0 3 2 0 0 0 0 1 2 0 2 6 7
Bud 1 2 – 2 1 4 2 – 2 5 8 4 1 5 1 2 0 0 0 0 0 1 1 1
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 – 0 0 1 0 – 1 3 1 1 0 0 1 1 0 0 – – – – – –
Cell Periphery 2 2 – 7 4 0 4 – 0 2 0 4 6 8 11 6 2 4 0 0 0 0 0 0
Cytoplasm 3 3 – 4 17 6 4 – 3 5 2 1 6 14 21 22 7 5 1 0 0 0 0 2
Endoplasmic Reticulum 3 5 – 2 2 1 2 – 3 2 0 0 3 2 6 5 4 1 0 1 0 0 1 6
Endosome 3 0 – 10 25 0 20 – 1 7 7 3 10 14 15 13 7 3 6 6 3 7 18 18
Golgi 3 1 – 26 41 0 8 – 2 4 1 1 31 41 34 12 5 8 5 7 1 6 17 27
Mitochondria 94 86 – 111 121 118 153 – 160 191 193 138 130 257 287 11 6 2 99 77 25 96 164 183
Nucleus 0 1 – 0 1 5 5 – 1 3 4 3 0 0 3 5 1 1 0 0 0 0 0 0
Nuclear Periphery 6 4 – 1 2 6 15 – 23 13 7 13 6 6 8 0 0 0 0 0 0 1 3 3
Nucleolus 4 6 – 1 4 6 15 – 14 18 16 13 4 4 2 1 0 0 0 0 0 0 1 3
Peroxisomes 2 0 – 3 0 0 1 – 3 0 1 3 5 11 10 7 0 3 6 2 4 12 24 25
SpindlePole 1 0 – 3 9 1 14 – 2 7 6 7 1 5 3 6 6 2 0 0 0 1 5 1
Vac/Vac Membrane 18 14 – 11 58 9 34 – 9 9 7 2 25 44 40 15 10 12 1 4 0 1 6 5
Unique Cell Count 104 98 141 210 136 210 180 225 210 161 162 300 354 89 37 35 124 106 38 134 258 293
Labelled Cell Count 140 126 186 285 158 277 224 269 255 193 231 413 442 106 48 41 124 106 38 134 258 293


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.3 – 7.0 8.1 4.9 6.9 – 5.6 6.1 6.1 6.2 7.3 6.9 6.9 8.2 8.5 8.3 6.5 7.0 7.1
Std Deviation (1e-4) 1.1 1.2 – 1.3 2.3 1.4 1.6 – 1.1 1.4 1.3 1.2 1.6 1.5 1.5 2.6 2.1 2.3 1.6 1.8 2.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7568 3.689 3.2515 2.8901 2.6741 2.9657 4.8509 5.0258 4.8381 4.4432 4.5083 4.8587 6.0087 6.2612 5.756 6.0255 5.0176 5.6989
Actin 0.0219 0.0058 0.0098 0.0506 0.0008 0.0013 0.0402 0.0052 0.0022 0.0005 0.0012 0.0176 0.0125 0.0032 0.0128 0.0001 0.0202 0.0005
Bud 0.0004 0.0002 0.0003 0.0011 0.0001 0 0.0005 0.0011 0.0005 0.0001 0.0002 0.0001 0.0002 0.0016 0.0002 0 0.0005 0
Bud Neck 0.0009 0.0001 0.0017 0.0022 0 0 0.0042 0.0003 0.0003 0 0 0.0001 0.0003 0.0002 0.0006 0 0.0001 0
Bud Periphery 0.0007 0.0004 0.0004 0.0019 0.0002 0.0001 0.0007 0.0014 0.0012 0.0003 0.0004 0.0002 0.0005 0.0042 0.0005 0 0.0021 0.0001
Bud Site 0.0018 0.0006 0.0066 0.0033 0.0001 0 0.0089 0.0071 0.001 0 0.0002 0.0002 0.0009 0.0061 0.0009 0 0.0004 0
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.0003 0.0007 0.0002 0 0.0003 0.0001
Cytoplasm 0.0007 0.0002 0.0012 0.0024 0 0 0.0043 0.0004 0.0003 0 0.0001 0.0001 0.0045 0.0002 0.0002 0 0.0008 0
Cytoplasmic Foci 0.0131 0.0077 0.0141 0.0243 0.0072 0.0008 0.0115 0.0078 0.0021 0.0019 0.0121 0.0021 0.0145 0.003 0.0059 0.0001 0.0022 0.0007
Eisosomes 0.0003 0.0001 0.0002 0.0005 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0002 0.0021 0 0.0003 0.0001
Endoplasmic Reticulum 0.0005 0.0008 0.0007 0.002 0 0 0.0018 0.0002 0.0006 0 0 0.0001 0.0016 0.0001 0.0001 0 0.001 0.0001
Endosome 0.0259 0.0322 0.0105 0.0455 0.0089 0.0033 0.0261 0.0153 0.0186 0.0014 0.0272 0.0094 0.0163 0.0077 0.0065 0.0004 0.0077 0.0048
Golgi 0.0337 0.0614 0.0155 0.0287 0.0142 0.0091 0.0255 0.0321 0.0214 0.0029 0.0189 0.0305 0.01 0.0124 0.0154 0.0008 0.0082 0.0123
Lipid Particles 0.0257 0.0047 0.0117 0.0138 0.006 0.0025 0.0572 0.0153 0.0059 0.0092 0.0076 0.0121 0.032 0.0096 0.012 0.0002 0.0054 0.0046
Mitochondria 0.8431 0.8483 0.8955 0.785 0.9068 0.9672 0.746 0.8797 0.9141 0.9706 0.8722 0.9099 0.8389 0.9149 0.9183 0.9972 0.8506 0.9628
None 0.0003 0.0001 0.0015 0.0021 0 0 0.0015 0.0032 0.0002 0 0 0.0001 0.0059 0.0001 0.0012 0 0.048 0
Nuclear Periphery 0.002 0.0044 0.0008 0.0027 0 0 0.0117 0.0018 0.0042 0 0 0.0001 0.0086 0.0001 0.0001 0 0.0007 0.0001
Nucleolus 0.0006 0.0001 0.0003 0.0004 0.0001 0 0.0005 0.0002 0.0003 0 0.0001 0 0.0008 0.0002 0.0001 0 0.0002 0
Nucleus 0.0008 0.0004 0.0002 0.0008 0 0 0.008 0.0003 0.0005 0 0 0 0.0023 0.0002 0.0001 0 0.0002 0
Peroxisomes 0.0208 0.024 0.0249 0.0257 0.0527 0.0136 0.0398 0.0147 0.0067 0.0124 0.0579 0.0144 0.0202 0.0243 0.0182 0.0007 0.0445 0.0114
Punctate Nuclear 0.0005 0 0.001 0.0024 0 0 0.0018 0.0002 0.0002 0 0.0001 0.0001 0.0057 0 0.0001 0 0.0002 0
Vacuole 0.0032 0.0036 0.0014 0.002 0.0011 0.0004 0.0048 0.004 0.0104 0.0001 0.0007 0.0006 0.0124 0.0051 0.0014 0.0001 0.0031 0.0004
Vacuole Periphery 0.0029 0.0046 0.0015 0.0022 0.0014 0.0013 0.0042 0.0091 0.0089 0.0003 0.001 0.0019 0.0111 0.0056 0.0032 0.0003 0.0034 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7575 20.2958 15.0604 31.208 22.0773 27.2295 26.6774 27.7007 24.9427 41.281
Translational Efficiency 2.6377 1.5543 2.009 1.081 1.4537 1.9089 1.7542 1.5468 1.9641 1.6564

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1458 1015 122 2201 1153 1999 273 1448 2611 3014 395 3649

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.93 838.22 1186.65 1050.87 730.73 878.51 998.66 1073.20 685.61 864.94 1056.72 1059.73
Standard Deviation 90.71 118.61 178.17 151.96 95.43 118.40 142.35 143.62 101.13 119.99 177.07 149.11
Intensity Change Log 2 — 0.367045 0.868538 0.693228 — 0.265720 0.450655 0.554509 — 0.314305 0.662480 0.621473

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000873 0.006532 0.007708 0.005709 0.000668 0.001839 0.005620 0.017797 0.000782 0.003419 0.006265 0.010506
Bud Neck 0.001557 0.002647 0.015344 0.000853 0.001530 0.000350 0.008800 0.001678 0.001545 0.001124 0.010821 0.001180
Bud Site 0.003524 0.009596 0.013739 0.008277 0.002940 0.001830 0.017555 0.015423 0.003266 0.004445 0.016376 0.011112
Cell Periphery 0.000467 0.000507 0.000582 0.000352 0.000235 0.000235 0.000759 0.001028 0.000364 0.000327 0.000705 0.000620
Cytoplasm 0.001500 0.002256 0.001138 0.001362 0.000978 0.000692 0.001592 0.003082 0.001270 0.001219 0.001452 0.002044
Cytoplasmic Foci 0.013601 0.041776 0.110030 0.012395 0.024295 0.013171 0.047354 0.016991 0.018323 0.022804 0.066712 0.014219
Eisosomes 0.000062 0.000102 0.000101 0.000076 0.000044 0.000045 0.000099 0.000133 0.000054 0.000065 0.000100 0.000099
Endoplasmic Reticulum 0.000630 0.000512 0.002540 0.000327 0.000330 0.000104 0.000635 0.000586 0.000498 0.000241 0.001223 0.000430
Endosome 0.023268 0.067801 0.131209 0.020313 0.042255 0.032250 0.082960 0.020044 0.031652 0.044222 0.097862 0.020206
Golgi 0.029934 0.064109 0.103536 0.048480 0.033972 0.030258 0.045532 0.053640 0.031717 0.041658 0.063447 0.050528
Lipid Particles 0.006838 0.009122 0.020404 0.000493 0.016105 0.001492 0.003844 0.000544 0.010930 0.004061 0.008958 0.000513
Mitochondria 0.878626* 0.713279* 0.360033* 0.860903* 0.826466* 0.893687* 0.672014* 0.828157* 0.855592* 0.832933* 0.575655* 0.847909*
Mitotic Spindle 0.002435 0.005934 0.099808 0.006968 0.004954 0.001287 0.027671 0.006523 0.003547 0.002852 0.049951 0.006792
None 0.000614 0.000215 0.000371 0.000326 0.000433 0.000142 0.000318 0.000325 0.000534 0.000167 0.000334 0.000326
Nuclear Periphery 0.000046 0.000103 0.004154 0.000122 0.000349 0.000028 0.000516 0.000085 0.000180 0.000053 0.001640 0.000107
Nuclear Periphery Foci 0.000206 0.000336 0.004401 0.000168 0.000624 0.000038 0.000957 0.000222 0.000390 0.000138 0.002021 0.000189
Nucleolus 0.000517 0.000234 0.000726 0.000304 0.000347 0.000056 0.001707 0.000042 0.000442 0.000116 0.001404 0.000200
Nucleus 0.001040 0.000454 0.001034 0.000200 0.000255 0.000410 0.001059 0.000119 0.000694 0.000425 0.001052 0.000168
Peroxisomes 0.027014 0.062624 0.026559 0.009409 0.033022 0.016544 0.051488 0.020660 0.029667 0.032062 0.043788 0.013874
Vacuole 0.002715 0.005601 0.047026 0.008963 0.004070 0.002711 0.017327 0.005894 0.003313 0.003684 0.026500 0.007745
Vacuole Periphery 0.004536 0.006260 0.049556 0.013999 0.006127 0.002830 0.012192 0.007026 0.005238 0.003985 0.023732 0.011232

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.52 -2.10 -5.61 0.55 0.59 -2.84 -3.30 -7.00 -6.44 -4.25 -5.21 -3.79 -8.79 -5.86 -2.34
Bud Neck -1.85 -2.22 2.24 3.27 2.33 3.16 -3.87 -0.39 -4.88 3.81 1.45 -4.03 1.27 -0.42 4.19
Bud Site -3.70 -2.95 -3.71 0.66 1.50 1.06 -3.57 -6.41 -7.58 0.40 -1.42 -4.36 -7.17 -6.15 1.62
Cell Periphery -0.43 -0.60 1.48 1.95 1.26 -0.01 -1.62 -2.95 -2.86 -0.65 0.53 -1.46 -2.21 -2.39 0.33
Cytoplasm -0.84 0.75 0.30 1.26 -0.99 0.50 -1.01 -2.51 -3.46 -2.18 0.16 -0.36 -1.66 -2.17 -1.86
Cytoplasmic Foci -9.27 -8.08 0.81 10.10 8.21 5.41 -3.62 3.02 -2.40 4.80 -2.81 -8.25 2.98 6.01 9.00
Eisosomes -2.75 -2.20 -0.95 2.73 1.80 -0.29 -4.39 -9.57 -9.54 -2.25 -1.30 -3.86 -4.80 -5.54 0.08
Endoplasmic Reticulum 0.45 -2.43 1.21 1.79 2.98 3.17 -2.53 -2.24 -5.04 0.34 1.75 -2.57 0.46 -3.36 3.21
Endosome -10.79 -9.09 1.38 11.82 9.37 2.87 -4.64 7.02 5.19 7.49 -5.19 -9.39 6.22 11.57 11.19
Golgi -8.05 -5.14 -5.27 3.40 3.81 1.24 -1.66 -4.65 -5.99 -1.10 -4.18 -4.79 -6.96 -3.19 1.91
Lipid Particles -1.11 -1.58 5.13 5.39 2.35 6.05 4.75 6.54 1.78 3.10 4.90 0.68 8.27 5.57 3.09
Mitochondria 13.05 17.49 2.10 -11.96 -16.97 -6.95 6.46 -0.08 7.30 -6.56 3.06 14.01 1.17 -2.07 -13.70
Mitotic Spindle -3.39 -5.28 -6.10 -0.93 5.04 3.25 -4.15 -1.16 -6.49 3.90 1.19 -6.63 -4.44 -6.38 6.17
None 1.65 1.06 1.25 -4.96 0.79 1.40 0.57 0.53 -5.39 -0.28 2.23 1.29 1.34 -6.85 0.28
Nuclear Periphery -1.85 -5.32 -3.99 -0.61 5.21 2.47 -1.04 1.95 -1.62 4.17 2.17 -5.46 1.18 -2.72 5.85
Nuclear Periphery Foci -1.12 -4.70 0.32 1.65 4.74 2.94 -1.22 1.60 -1.55 3.14 2.61 -4.96 1.66 -1.19 5.69
Nucleolus 1.16 -0.40 0.71 -0.28 1.10 2.50 -1.47 2.73 0.30 1.77 2.15 -1.42 1.22 -0.57 1.79
Nucleus 1.18 0.12 1.76 0.99 1.90 -0.48 -1.52 1.31 0.81 1.71 0.83 -0.73 1.89 1.03 2.16
Peroxisomes -6.58 0.12 6.41 10.86 3.62 4.90 -2.07 3.26 -1.43 3.53 -0.84 -2.23 7.05 7.96 4.89
Vacuole -3.52 -5.27 -6.76 -3.35 4.50 2.25 -5.63 -2.10 -3.89 4.72 -0.82 -7.39 -6.81 -6.37 5.90
Vacuole Periphery -1.80 -4.74 -7.37 -5.41 3.72 5.22 -3.53 -0.95 -5.05 2.87 2.49 -5.66 -6.94 -8.56 3.74
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl49

Mrpl49


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl49-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available