Standard name
Human Ortholog
Description Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0.13 0.05 0 0.05 0 0 0 0.07 0 0 0 0
Bud – 0.09 0.13 0.12 0.09 0.14 0.14 0.17 – 0.13 0.13 0.16 0.07 0.07 0 0.09 0.06 0.07 0 0 0 0 0 0
Bud Neck – 0 0.11 0.09 0.08 0.14 0.11 0.08 – 0.06 0.05 0.06 0.16 0.13 0.12 0.05 0.08 0.05 0 0 0 0 0 0
Bud Site – 0.06 0 0.06 0.05 0.08 0 0.05 – 0.06 0.07 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.25 0.29 0.21 0.23 0.25 0.21 0.19 – 0.18 0.14 0.11 0.37 0.34 0.33 0.11 0.23 0.13 0.09 0.09 0.06 0.07 0.07 0.06
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.07 0.09 0.12 0.12 0.13 0.17 0.21 0.2 0.17 0.16
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.62 0.17 0.16 0.16 0.29 0.4 0.35 – 0.38 0.5 0.47 0 0 0 0.18 0.06 0.07 0.16 0.25 0.13 0.15 0.13 0.14
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0.1 0.06 0 0 0 – 0 0 0.08 0 0 0 0.06 0.08 0.06 0.1 0.05 0.13 0.15 0.16 0.14
SpindlePole – 0.17 0.49 0.59 0.59 0.5 0.46 0.54 – 0.54 0.48 0.56 0.45 0.54 0.56 0.66 0.54 0.74 0.39 0.28 0.37 0.33 0.35 0.34
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 1 2 1 7 0 0 – 0 0 0 23 14 5 6 3 3 0 5 5 1 6 14
Bud – 5 27 29 32 62 74 98 – 74 59 92 12 18 10 11 5 8 0 0 0 0 4 4
Bud Neck – 0 23 22 30 62 57 48 – 32 23 33 29 35 34 6 7 6 0 1 4 3 6 12
Bud Site – 3 8 15 17 34 23 32 – 37 30 53 2 3 3 4 2 5 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 13 61 52 80 111 112 111 – 104 63 64 66 90 97 14 19 16 2 8 12 10 21 21
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 1 0 2 2 4 3 0 0 0 0 0 3
Endosome – 1 1 6 6 0 1 1 – 5 1 1 0 12 20 11 10 15 4 15 48 30 50 53
Golgi – 0 1 0 0 0 0 0 – 0 0 0 0 0 2 0 2 2 0 0 0 1 0 2
Mitochondria – 33 36 38 58 131 212 204 – 217 229 276 2 0 0 23 5 8 5 21 29 22 39 46
Nucleus – 0 0 1 1 4 4 2 – 1 2 2 1 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 2 2 0 1 4 2 11 – 7 5 5 0 0 0 1 2 1 0 0 1 0 2 1
Peroxisomes – 1 8 23 20 10 14 6 – 17 16 47 0 8 9 7 7 7 3 4 29 22 47 45
SpindlePole – 9 104 142 210 221 247 313 – 312 221 332 81 141 165 84 45 90 11 24 85 49 104 114
Vac/Vac Membrane – 1 0 0 2 1 0 0 – 1 0 0 0 0 1 2 2 1 0 1 1 2 8 5
Unique Cell Count 53 211 242 353 446 532 582 576 458 591 179 262 295 127 83 122 31 88 230 149 299 332
Labelled Cell Count 69 272 330 458 647 746 826 807 652 905 218 321 348 172 114 165 31 88 230 149 299 332


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.5 3.1 2.9 2.9 3.0 2.3 2.5 – 2.4 2.4 2.5 4.7 4.7 4.9 5.0 5.5 5.2 4.9 5.3 5.2
Std Deviation (1e-4) – 0.4 1.0 1.2 0.8 1.0 0.8 0.7 – 0.7 0.7 0.6 0.7 0.7 0.9 1.3 2.2 1.6 1.0 1.6 1.5
Intensity Change (Log2) – – – -0.07 -0.06 -0.04 -0.39 -0.27 – -0.35 -0.37 -0.31 0.6 0.61 0.66 0.71 0.85 0.77 0.67 0.79 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.644 -0.499 -0.5756 -0.5882 -0.8315 -0.4965 0.609 1.2781 0.935 0.7312 0.7176 1.0227 -1.7561 -1.3091 -1.6648 -1.3652 -1.7533 -1.6298
Actin 0.0123 0.0089 0.0075 0.0033 0.0058 0.0063 0.0225 0.0171 0.0187 0.0051 0.0123 0.0084 0.0206 0.0035 0.004 0.0064 0.0091 0.0033
Bud 0.0002 0.0013 0.0003 0.0001 0.0002 0.0001 0.0006 0.0013 0.0012 0.0001 0.0011 0.0001 0.0001 0.0001 0.0002 0.0002 0 0
Bud Neck 0.0076 0.0017 0.0045 0.0097 0.001 0.0051 0.0045 0.0031 0.0069 0.0116 0.0052 0.0062 0.0013 0.0008 0.0064 0.0047 0.0005 0.004
Bud Periphery 0.0005 0.0015 0.0008 0.0003 0.0005 0.0002 0.0022 0.0056 0.0018 0.0001 0.0028 0.0003 0.0001 0.0002 0.0004 0.0006 0.0001 0.0001
Bud Site 0.0085 0.0107 0.0043 0.0022 0.0002 0.0013 0.0025 0.0067 0.005 0.0002 0.0173 0.0002 0.0011 0.004 0.0046 0.0008 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0006 0.0004 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0089 0.0105 0.009 0.0061 0.0208 0.0063 0.0158 0.017 0.0196 0.0104 0.0094 0.0113 0.0155 0.0103 0.0121 0.0066 0.0074 0.0129
Cytoplasmic Foci 0.3944 0.3109 0.3324 0.3087 0.3143 0.4061 0.4412 0.362 0.3764 0.3771 0.3941 0.4497 0.4278 0.4255 0.4488 0.3919 0.4796 0.5306
Eisosomes 0.0006 0.0009 0.0005 0.0002 0.0002 0.0002 0.0006 0.0007 0.0008 0.0007 0.0006 0.0005 0.0007 0.0003 0.0004 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0015 0.0003 0.0004 0.0002 0.0004 0.0002 0.002 0.0008 0.0008 0.0001 0.0005 0.0004 0.0026 0.0006 0.0005 0.0005 0.0001 0.0003
Endosome 0.0235 0.0166 0.0184 0.016 0.0175 0.0099 0.0378 0.0081 0.0209 0.0044 0.0205 0.0138 0.0293 0.0228 0.0181 0.0256 0.0032 0.0132
Golgi 0.005 0.0048 0.0061 0.0055 0.0034 0.0038 0.007 0.0047 0.0061 0.0037 0.0128 0.0046 0.0077 0.0063 0.0069 0.0065 0.0035 0.0046
Lipid Particles 0.051 0.0367 0.0302 0.0368 0.0285 0.0413 0.0567 0.023 0.0221 0.0246 0.0316 0.0389 0.0659 0.0508 0.0376 0.0689 0.0233 0.0558
Mitochondria 0.0031 0.0041 0.0093 0.0027 0.0041 0.0037 0.0071 0.009 0.0088 0.0034 0.0064 0.003 0.0028 0.0046 0.006 0.0058 0.0008 0.0025
None 0.2045 0.3291 0.2856 0.3343 0.2603 0.2156 0.1843 0.2634 0.2917 0.1755 0.2153 0.197 0.2415 0.2562 0.2226 0.1806 0.117 0.1231
Nuclear Periphery 0.0026 0.0004 0.0006 0.0004 0.0007 0.0003 0.0047 0.0012 0.0007 0.0003 0.0003 0.0014 0.0029 0.0004 0.0005 0.001 0.0001 0.0002
Nucleolus 0.0028 0.0018 0.0014 0.0009 0.0004 0.001 0.0011 0.0009 0.001 0.0005 0.0002 0.0022 0.0009 0.0005 0.0006 0.0006 0.0001 0.0003
Nucleus 0.0035 0.0008 0.0007 0.0005 0.0008 0.0006 0.0021 0.0007 0.0006 0.0009 0.0002 0.0007 0.0011 0.0003 0.0007 0.0004 0 0.0003
Peroxisomes 0.2224 0.2414 0.2551 0.2509 0.3052 0.2587 0.1857 0.2422 0.1977 0.3628 0.2622 0.2305 0.145 0.2037 0.2106 0.2629 0.3406 0.2209
Punctate Nuclear 0.0455 0.0164 0.032 0.0206 0.0342 0.0386 0.0191 0.0308 0.0171 0.0184 0.0059 0.0303 0.0318 0.0083 0.0182 0.0335 0.0142 0.0272
Vacuole 0.001 0.0009 0.0006 0.0003 0.0012 0.0005 0.0018 0.0009 0.0014 0.0001 0.0007 0.0004 0.0008 0.0006 0.0007 0.002 0 0.0002
Vacuole Periphery 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0005 0.0003 0.0004 0 0.0003 0.0001 0.0003 0.0002 0.0002 0.0005 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.4372 9.6704 7.1758 8.5646 2.6298 2.7029 4.8888 8.2491 6.2436 6.6858
Translational Efficiency 0.6342 0.51 0.7684 0.7702 1.2738 1.7709 1.095 0.861 1.0227 0.7882

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
512 81 653 1110 1515 141 2442 2052 2027 222 3095 3162

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 619.71 713.88 773.07 744.73 608.43 664.27 735.07 781.63 611.28 682.37 743.09 768.68
Standard Deviation 65.18 101.08 73.44 89.86 94.83 95.58 76.91 95.17 88.42 100.50 77.75 94.99
Intensity Change Log 2 — 0.204088 0.319006 0.265124 — 0.126678 0.272790 0.361395 — 0.166258 0.296295 0.313620

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000393 0.001301 0.001039 0.001227 0.000265 0.000621 0.000325 0.001807 0.000298 0.000869 0.000476 0.001603
Bud Neck 0.062093 0.063799 0.024565 0.020069 0.052273 0.054476 0.054253 0.060586 0.054753 0.057878 0.047989 0.046363
Bud Site 0.005445 0.042723 0.023106 0.027661 0.006259 0.006145 0.061633 0.053324 0.006053 0.019491 0.053505 0.044315
Cell Periphery 0.000078 0.000193 0.000155 0.000098 0.000151 0.000337 0.000090 0.000253 0.000133 0.000285 0.000104 0.000199
Cytoplasm 0.101077* 0.088400 0.123874* 0.250356* 0.109022* 0.062371 0.095415 0.135604* 0.107016* 0.071868 0.101420* 0.175887*
Cytoplasmic Foci 0.421931* 0.284138* 0.396408* 0.176577 0.399072* 0.273188* 0.410508* 0.226949* 0.404846* 0.277183* 0.407533* 0.209267*
Eisosomes 0.000159 0.000230 0.000113 0.000106 0.000261 0.000600 0.000043 0.000125 0.000236 0.000465 0.000058 0.000119
Endoplasmic Reticulum 0.000267 0.000181 0.000668 0.000669 0.000183 0.000369 0.000151 0.000574 0.000204 0.000300 0.000260 0.000607
Endosome 0.060257 0.090043 0.089871 0.035558 0.095697 0.091897 0.059664 0.057021 0.086746 0.091220 0.066037 0.049487
Golgi 0.010384 0.019688 0.005599 0.005656 0.014688 0.039471 0.011571 0.004838 0.013601 0.032253 0.010311 0.005125
Lipid Particles 0.029959 0.006722 0.010651 0.002105 0.023373 0.035926 0.001316 0.005952 0.025037 0.025270 0.003286 0.004601
Mitochondria 0.020956 0.005802 0.000987 0.006161 0.010441 0.057154 0.001345 0.005135 0.013097 0.038417 0.001269 0.005495
Mitotic Spindle 0.005795 0.015293 0.028970 0.043466 0.004074 0.013145 0.046882 0.049651 0.004509 0.013929 0.043102 0.047480
None 0.001394 0.000557 0.002497 0.002413 0.005075 0.001220 0.001186 0.002658 0.004145 0.000978 0.001463 0.002572
Nuclear Periphery 0.000351 0.000081 0.000490 0.000590 0.000443 0.000463 0.000137 0.000582 0.000419 0.000323 0.000211 0.000585
Nuclear Periphery Foci 0.000246 0.000807 0.005727 0.000879 0.000559 0.000347 0.002285 0.001236 0.000480 0.000515 0.003011 0.001111
Nucleolus 0.003644 0.002657 0.011521 0.001509 0.006767 0.004242 0.004375 0.006103 0.005978 0.003664 0.005883 0.004490
Nucleus 0.043475 0.030413 0.072195 0.052435 0.048715 0.028297 0.016227 0.103112 0.047391 0.029069 0.028035 0.085322
Peroxisomes 0.213006* 0.311018* 0.170577* 0.117630* 0.205423* 0.313172* 0.218738* 0.242328* 0.207338* 0.312386* 0.208577* 0.198553*
Vacuole 0.018596 0.035051 0.030486 0.253054* 0.016582 0.014781 0.013628 0.041232 0.017091 0.022176 0.017184 0.115591
Vacuole Periphery 0.000493 0.000904 0.000501 0.001779 0.000676 0.001781 0.000229 0.000931 0.000630 0.001461 0.000286 0.001229

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.26 -1.72 -4.35 0.82 -0.04 -1.96 -1.20 -7.16 -4.25 -6.90 -3.07 -2.02 -8.27 -2.79 -6.63
Bud Neck 0.05 6.44 8.18 3.06 2.93 -0.23 -0.37 -2.65 -0.63 -2.67 -0.29 2.24 2.47 1.13 0.25
Bud Site -3.00 -5.80 -7.60 1.80 0.46 0.02 -21.28 -16.53 -11.54 2.52 -2.69 -21.89 -17.93 -3.82 4.27
Cell Periphery -1.55 -4.15 0.26 1.62 5.67 -2.92 4.44 -4.00 1.65 -8.10 -3.12 2.66 -3.08 2.19 -6.09
Cytoplasm -0.05 -1.89 -9.87 -4.90 -7.81 3.51 1.39 -4.18 -5.75 -6.00 2.56 0.39 -9.46 -7.42 -10.52
Cytoplasmic Foci 5.61 1.91 25.95 6.49 22.82 7.04 -0.72 22.74 3.04 24.60* 8.89 0.27 33.67 6.27 34.47*
Eisosomes -1.10 2.10 2.74 1.90 1.32 -2.06 7.39 3.91 2.79 -17.56 -2.13 7.80 4.55 3.12 -16.48
Endoplasmic Reticulum 0.21 -3.56 -6.12 -6.92 -1.65 -1.09 -0.24 -9.06 -3.46 -9.92 -1.01 -2.22 -11.36 -5.33 -11.07
Endosome -1.84 -3.33 7.44 4.08 10.53 0.25 7.34 8.74 2.95 2.12 -0.60 5.13 11.78 4.81 8.44
Golgi -1.81 4.64 7.09 3.68 4.05 -3.21 3.50 10.34 4.60 14.30 -3.50 4.54 12.29 5.48 14.69
Lipid Particles 3.64 4.69 7.43 1.60 9.06 -1.41 13.01 9.14 3.44 -13.65 -0.09 13.51 12.13 3.70 -5.53
Mitochondria 4.53 7.46 6.67 1.26 -1.49 -3.27 8.76 5.15 3.69 -6.52 -2.69 11.44 8.37 3.73 -5.63
Mitotic Spindle -1.10 -6.43 -6.72 -1.66 1.20 -1.16 -17.46 -14.46 -5.81 0.08 -1.62 -18.50 -15.80 -5.64 1.42
None 2.10 -2.74 -0.22 -2.39 2.64 2.96 4.67 3.22 -0.80 -3.13 3.52 4.07 3.33 -1.28 -1.52
Nuclear Periphery 1.85 -1.67 -5.58 -14.43 -6.84 -0.12 1.54 -5.22 -3.48 -13.58 0.54 0.96 -7.03 -6.52 -16.58
Nuclear Periphery Foci -1.74 -5.06 -7.06 0.57 4.60 2.24 -5.58 -5.83 -7.36 2.16 -0.73 -7.46 -7.06 -2.94 5.15
Nucleolus 0.94 -4.85 3.32 1.81 6.58 2.01 0.53 -2.69 -3.89 -3.47 2.45 -2.29 -0.93 -3.20 1.43
Nucleus 0.88 -3.34 -4.27 -2.79 -0.05 2.33 8.31 -11.43 -9.33 -18.53 2.38 5.47 -11.90 -8.99 -17.71
Peroxisomes -2.50 4.49 12.92 7.31 9.91 -4.53 0.48 -0.10 4.51 -0.60 -5.19 2.40 7.63 7.86 6.12
Vacuole -1.65 -3.54 -39.84 -25.81 -38.35 0.53 -0.21 -15.70 -10.96 -15.62 -1.24 -1.81 -33.91 -24.36 -33.34
Vacuole Periphery -1.13 0.24 -0.87 0.56 -0.96 -2.72 6.57 -0.46 2.52 -4.42 -2.85 6.42 -0.73 2.48 -4.24
Standard name
Human Ortholog
Description Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdm12

Mdm12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available