Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | 2 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 4 | 7 | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 6 | 8 | 2 | 11 | 23 | 16 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 14 | 38 | 36 | 42 | 105 | 152 | 149 | 184 | 151 | 138 | 141 | 207 | 606 | 714 | 53 | 47 | 35 | 1 | 1 | 0 | 11 | 21 | 32 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 4 | 4 | 1 | 18 | 6 | 9 | 0 | 0 | 0 | 0 | 3 | 3 |
Endosome | 0 | 9 | 6 | 5 | 7 | 3 | 1 | 7 | 2 | 4 | 0 | 1 | 43 | 31 | 9 | 18 | 9 | 7 | 7 | 6 | 5 | 10 | 22 | 19 |
Golgi | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 7 | 3 | 4 | 0 | 1 | 1 | 2 | 2 | 1 |
Mitochondria | 52 | 8 | 9 | 6 | 16 | 26 | 56 | 18 | 50 | 33 | 44 | 26 | 6 | 1 | 1 | 7 | 10 | 8 | 4 | 2 | 1 | 3 | 10 | 12 |
Nucleus | 46 | 37 | 57 | 54 | 75 | 65 | 53 | 70 | 61 | 33 | 26 | 26 | 27 | 18 | 9 | 200 | 116 | 162 | 8 | 7 | 4 | 6 | 16 | 5 |
Nuclear Periphery | 4 | 0 | 2 | 1 | 1 | 1 | 4 | 0 | 1 | 3 | 3 | 0 | 1 | 0 | 0 | 4 | 0 | 2 | 1 | 1 | 2 | 2 | 4 | 4 |
Nucleolus | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 4 | 0 | 0 | 5 | 7 | 0 | 2 | 2 | 0 | 1 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 3 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 9 | 9 | 3 | 4 | 7 | 2 | 1 | 0 | 1 | 9 | 13 | 14 |
Vac/Vac Membrane | 105 | 128 | 97 | 84 | 199 | 170 | 183 | 193 | 202 | 173 | 121 | 149 | 193 | 159 | 72 | 251 | 167 | 214 | 161 | 159 | 175 | 266 | 584 | 453 |
Unique Cell Count | 182 | 170 | 177 | 152 | 286 | 285 | 359 | 369 | 390 | 322 | 260 | 278 | 433 | 776 | 777 | 474 | 303 | 374 | 201 | 196 | 197 | 331 | 714 | 571 |
Labelled Cell Count | 238 | 200 | 216 | 189 | 343 | 373 | 458 | 445 | 510 | 407 | 338 | 349 | 501 | 834 | 812 | 569 | 373 | 443 | 201 | 196 | 197 | 331 | 714 | 571 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 9.2 | 7.3 | 6.9 | 7.9 | 6.5 | 6.2 | 6.9 | 5.8 | 6.1 | 5.5 | 5.9 | 5.9 | 5.4 | 5.2 | 11.1 | 11.6 | 10.8 | 6.4 | 6.3 | 6.3 |
Std Deviation (1e-4) | 2.8 | 4.5 | 2.7 | 2.6 | 2.6 | 2.9 | 2.8 | 2.7 | 2.7 | 2.6 | 2.4 | 2.3 | 1.8 | 1.5 | 1.5 | 5.2 | 5.5 | 5.1 | 2.4 | 2.1 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.11 | -0.16 | -0.24 | -0.08 | -0.33 | -0.25 | -0.41 | -0.31 | -0.3 | -0.44 | -0.48 | 0.61 | 0.67 | 0.57 | -0.19 | -0.21 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -1.9 | 3.5 | 4.8 | 4.4 | 5.8 | 5.6 | 6.6 | 6.2 | 6.0 | 14.5 | 20.7 | -3.4 | -1.6 | -3.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 |
Endosome | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0.4 | 0 | 0.2 | -0.3 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.5 | 0.2 | 1.6 | 3.5 | -0.1 | 2.8 | 2.0 | 3.7 | 1.7 | 0 | 0 | 0 | 0 | -1.0 | -1.9 |
Nucleus | 0.6 | -1.4 | -2.2 | -4.7 | -3.4 | -4.5 | -6.1 | -5.8 | -6.2 | -8.4 | -13.3 | -14.7 | 2.3 | 1.3 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.2 | 1.0 | -0.8 | -0.5 | -0.7 | -0.2 | -1.7 | -0.3 | -2.3 | -9.3 | -14.3 | -0.4 | 0.1 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3054 | 5.1282 | 3.9979 | 2.2602 | 2.1631 | 3.0567 | 1.0751 | 3.8964 | 4.2934 | 2.729 | 3.1627 | 3.9222 | 0.8408 | 7.0001 | 5.3789 | 3.7257 | 3.2588 | 4.19 |
Actin | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0274 | 0.0002 | 0.0111 | 0.0005 | 0 | 0.0134 | 0.0207 | 0.0004 | 0.0009 | 0.0049 | 0.0065 | 0.0004 |
Bud | 0.001 | 0.0042 | 0.0009 | 0.0009 | 0.0007 | 0.0007 | 0.0007 | 0.0013 | 0.001 | 0.0008 | 0.0002 | 0.0005 | 0.0035 | 0.0023 | 0.0007 | 0.0008 | 0.0004 | 0.0006 |
Bud Neck | 0.0009 | 0.0022 | 0.0029 | 0.0019 | 0.0023 | 0.0074 | 0.0018 | 0.0026 | 0.0023 | 0.0009 | 0.0008 | 0.0027 | 0.0035 | 0.0019 | 0.0048 | 0.0086 | 0.0016 | 0.0135 |
Bud Periphery | 0.0028 | 0.0056 | 0.0027 | 0.0025 | 0.0027 | 0.0028 | 0.0019 | 0.0023 | 0.0031 | 0.0012 | 0.0005 | 0.0018 | 0.0054 | 0.0038 | 0.0022 | 0.0017 | 0.0012 | 0.0024 |
Bud Site | 0.0013 | 0.0055 | 0.0029 | 0.0014 | 0.0017 | 0.0011 | 0.0022 | 0.008 | 0.0022 | 0.0047 | 0.0004 | 0.0008 | 0.0072 | 0.011 | 0.0022 | 0.0114 | 0.0009 | 0.0006 |
Cell Periphery | 0.0043 | 0.0083 | 0.0069 | 0.0039 | 0.0087 | 0.0078 | 0.0025 | 0.0058 | 0.0065 | 0.0026 | 0.0027 | 0.0064 | 0.0041 | 0.0083 | 0.0097 | 0.0076 | 0.0079 | 0.0065 |
Cytoplasm | 0.0029 | 0.0103 | 0.0024 | 0.0008 | 0.0008 | 0.0034 | 0.0095 | 0.0111 | 0.002 | 0.0011 | 0.0006 | 0.0009 | 0.0027 | 0.0069 | 0.0014 | 0.0009 | 0.0011 | 0.0015 |
Cytoplasmic Foci | 0.0184 | 0.0132 | 0.0096 | 0.0065 | 0.0029 | 0.0104 | 0.0303 | 0.0101 | 0.0071 | 0.0196 | 0.0019 | 0.0035 | 0.0258 | 0.0091 | 0.0154 | 0.0098 | 0.0026 | 0.0041 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.002 | 0.0014 | 0.0006 | 0.0018 | 0.0013 | 0.0051 | 0.0027 | 0.0025 | 0.0006 | 0.0003 | 0.0011 | 0.0021 | 0.0022 | 0.0016 | 0.0006 | 0.0015 | 0.0022 |
Endosome | 0.2013 | 0.0763 | 0.1255 | 0.1106 | 0.0681 | 0.1021 | 0.1989 | 0.1341 | 0.1003 | 0.1061 | 0.0715 | 0.1095 | 0.1629 | 0.091 | 0.0972 | 0.1402 | 0.1021 | 0.0893 |
Golgi | 0.011 | 0.009 | 0.0097 | 0.0049 | 0.007 | 0.0092 | 0.016 | 0.0074 | 0.015 | 0.0134 | 0.0012 | 0.0091 | 0.02 | 0.0168 | 0.0126 | 0.0105 | 0.0065 | 0.0095 |
Lipid Particles | 0.0449 | 0.0136 | 0.0397 | 0.012 | 0.0339 | 0.033 | 0.0556 | 0.0204 | 0.0478 | 0.022 | 0.005 | 0.0162 | 0.0385 | 0.0127 | 0.0335 | 0.0329 | 0.0076 | 0.0215 |
Mitochondria | 0.0572 | 0.0118 | 0.0668 | 0.0148 | 0.0057 | 0.0223 | 0.0318 | 0.0102 | 0.061 | 0.0125 | 0.0012 | 0.0158 | 0.0368 | 0.0164 | 0.0454 | 0.0162 | 0.0034 | 0.0178 |
None | 0.0072 | 0.0044 | 0.0002 | 0.0002 | 0.0001 | 0.0035 | 0.0074 | 0.0033 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.008 | 0.0012 | 0.0003 | 0.0002 | 0.0007 | 0.0023 |
Nuclear Periphery | 0.0032 | 0.0052 | 0.0054 | 0.0012 | 0.0038 | 0.0031 | 0.015 | 0.0043 | 0.0053 | 0.0013 | 0.0007 | 0.0016 | 0.0067 | 0.0038 | 0.0032 | 0.001 | 0.0149 | 0.0039 |
Nucleolus | 0.0075 | 0.0098 | 0.0139 | 0.0074 | 0.0055 | 0.0129 | 0.0194 | 0.0055 | 0.0054 | 0.0043 | 0.0045 | 0.0038 | 0.015 | 0.0047 | 0.006 | 0.0074 | 0.0061 | 0.0064 |
Nucleus | 0.0062 | 0.0151 | 0.0068 | 0.0036 | 0.0045 | 0.0095 | 0.0071 | 0.0045 | 0.0034 | 0.0027 | 0.0021 | 0.0038 | 0.009 | 0.0042 | 0.0029 | 0.0022 | 0.0037 | 0.0091 |
Peroxisomes | 0.0098 | 0.0027 | 0.0038 | 0.0021 | 0.0008 | 0.0025 | 0.0178 | 0.001 | 0.0024 | 0.007 | 0.0004 | 0.0008 | 0.0086 | 0.0016 | 0.0045 | 0.0058 | 0.0011 | 0.0016 |
Punctate Nuclear | 0.0007 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0152 | 0.0014 | 0.0003 | 0.0018 | 0 | 0.0001 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0017 | 0.0001 |
Vacuole | 0.4324 | 0.6307 | 0.5265 | 0.6355 | 0.6969 | 0.588 | 0.3935 | 0.5961 | 0.5337 | 0.6316 | 0.6899 | 0.6633 | 0.4324 | 0.5954 | 0.5321 | 0.5768 | 0.6821 | 0.6103 |
Vacuole Periphery | 0.1852 | 0.169 | 0.1714 | 0.1891 | 0.1518 | 0.1781 | 0.1407 | 0.1676 | 0.1868 | 0.1651 | 0.2158 | 0.1446 | 0.1853 | 0.2062 | 0.2231 | 0.1601 | 0.1464 | 0.1963 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 97.3276 | 16.3435 | 6.4205 | 18.0195 | 73.2038 | 51.5927 | 15.5528 | 13.4494 | 11.13 | 84.5632 |
Translational Efficiency | 1.371 | 1.5981 | 0.8472 | 0.6251 | 1.4084 | 2.7052 | 1.6104 | 0.8037 | 1.176 | 1.2486 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1360 | 769 | 1935 | 1542 | 1889 | 1199 | 162 | 102 | 3249 | 1968 | 2097 | 1644 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 813.42 | 920.75 | 729.80 | 917.38 | 786.08 | 907.39 | 925.51 | 1092.33 | 797.52 | 912.61 | 744.92 | 928.23 |
Standard Deviation | 176.53 | 212.90 | 142.42 | 188.18 | 146.63 | 168.21 | 132.88 | 518.89 | 160.40 | 187.06 | 151.04 | 227.38 |
Intensity Change Log 2 | — | 0.178809 | -0.156499 | 0.173519 | — | 0.207047 | 0.235572 | 0.474661 | — | 0.192756 | 0.049480 | 0.329366 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000230 | 0.000715 | 0.001171 | 0.003974 | 0.000079 | 0.000158 | 0.000811 | 0.001434 | 0.000142 | 0.000376 | 0.001143 | 0.003817 |
Bud Neck | 0.019508 | 0.017753 | 0.096385 | 0.038399 | 0.020590 | 0.015058 | 0.051265 | 0.054501 | 0.020137 | 0.016111 | 0.092900 | 0.039398 |
Bud Site | 0.044074 | 0.051409 | 0.190856* | 0.166671* | 0.029940 | 0.023493 | 0.061778 | 0.110789* | 0.035856 | 0.034401 | 0.180884* | 0.163204* |
Cell Periphery | 0.009394 | 0.007186 | 0.000495 | 0.001230 | 0.006238 | 0.004225 | 0.000331 | 0.001266 | 0.007559 | 0.005382 | 0.000482 | 0.001232 |
Cytoplasm | 0.023848 | 0.020710 | 0.211775* | 0.063385 | 0.016044 | 0.011304 | 0.086224 | 0.086298 | 0.019311 | 0.014979 | 0.202076* | 0.064806 |
Cytoplasmic Foci | 0.011626 | 0.011658 | 0.031731 | 0.021618 | 0.005951 | 0.007355 | 0.034637 | 0.051765 | 0.008326 | 0.009036 | 0.031956 | 0.023489 |
Eisosomes | 0.000081 | 0.000050 | 0.000052 | 0.000050 | 0.000038 | 0.000070 | 0.000084 | 0.000046 | 0.000056 | 0.000063 | 0.000054 | 0.000050 |
Endoplasmic Reticulum | 0.004072 | 0.003169 | 0.002166 | 0.002079 | 0.004162 | 0.004866 | 0.003588 | 0.001185 | 0.004124 | 0.004203 | 0.002276 | 0.002024 |
Endosome | 0.055162 | 0.055438 | 0.060799 | 0.105888 | 0.045207 | 0.046763 | 0.119287 | 0.120920 | 0.049374 | 0.050153 | 0.065317 | 0.106820 |
Golgi | 0.005687 | 0.005554 | 0.006660 | 0.036542 | 0.002696 | 0.004492 | 0.004768 | 0.066633 | 0.003948 | 0.004907 | 0.006514 | 0.038409 |
Lipid Particles | 0.001105 | 0.001465 | 0.001487 | 0.001420 | 0.001205 | 0.001233 | 0.002928 | 0.019509 | 0.001163 | 0.001324 | 0.001598 | 0.002543 |
Mitochondria | 0.003951 | 0.005000 | 0.004677 | 0.010012 | 0.003759 | 0.007881 | 0.001744 | 0.004619 | 0.003840 | 0.006755 | 0.004450 | 0.009677 |
Mitotic Spindle | 0.000312 | 0.000415 | 0.027711 | 0.019682 | 0.000618 | 0.002503 | 0.007884 | 0.027048 | 0.000490 | 0.001687 | 0.026179 | 0.020139 |
None | 0.001265 | 0.000353 | 0.006564 | 0.000501 | 0.000464 | 0.000343 | 0.002590 | 0.000521 | 0.000800 | 0.000347 | 0.006257 | 0.000502 |
Nuclear Periphery | 0.000150 | 0.000207 | 0.000840 | 0.000751 | 0.000124 | 0.000328 | 0.001480 | 0.002831 | 0.000135 | 0.000281 | 0.000889 | 0.000880 |
Nuclear Periphery Foci | 0.000245 | 0.000344 | 0.001854 | 0.001109 | 0.000172 | 0.000402 | 0.003136 | 0.001993 | 0.000203 | 0.000380 | 0.001953 | 0.001164 |
Nucleolus | 0.000733 | 0.001544 | 0.004289 | 0.001219 | 0.000607 | 0.001713 | 0.006421 | 0.001541 | 0.000660 | 0.001647 | 0.004454 | 0.001239 |
Nucleus | 0.006183 | 0.006519 | 0.124947 | 0.015129 | 0.005321 | 0.012043 | 0.277605* | 0.013457 | 0.005682 | 0.009885 | 0.136740* | 0.015025 |
Peroxisomes | 0.001263 | 0.003548 | 0.017696 | 0.008695 | 0.000874 | 0.000790 | 0.015266 | 0.014669 | 0.001037 | 0.001868 | 0.017508 | 0.009065 |
Vacuole | 0.749651* | 0.719886* | 0.201511* | 0.461513* | 0.789284* | 0.785215* | 0.302356* | 0.383289* | 0.772694* | 0.759688* | 0.209301* | 0.456659* |
Vacuole Periphery | 0.061459 | 0.087076 | 0.006334 | 0.040133 | 0.066626 | 0.069764 | 0.015818 | 0.035683 | 0.064463 | 0.076529 | 0.007067 | 0.039857 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.46 | -5.72 | -4.66 | -3.62 | -3.33 | -1.22 | -3.02 | -4.44 | -4.10 | -1.37 | -1.72 | -7.15 | -4.90 | -4.46 | -3.38 |
Bud Neck | 0.66 | -18.55 | -6.81 | -6.67 | 13.30 | 2.57 | -3.15 | -2.04 | -2.65 | 0.56 | 2.42 | -19.29 | -7.47 | -9.25 | 13.03 |
Bud Site | -1.24 | -18.72 | -15.03 | -12.59 | 1.86 | 1.91 | -1.98 | -3.51 | -3.89 | -2.02 | 0.48 | -21.48 | -17.59 | -17.41 | 1.32 |
Cell Periphery | 1.67 | 12.07 | 10.70 | 5.34 | -2.72 | 2.67 | 9.68 | 7.34 | 5.63 | -1.47 | 3.13 | 15.03 | 12.44 | 7.71 | -2.88 |
Cytoplasm | 0.71 | -30.16 | -13.59 | -13.31 | 16.90 | 1.76 | -6.69 | -5.58 | -5.78 | -1.50 | 1.87 | -32.43 | -16.35 | -16.95 | 15.95 |
Cytoplasmic Foci | -0.04 | -8.21 | -4.22 | -3.66 | 4.77 | -1.04 | -4.69 | -2.77 | -2.62 | -0.42 | -0.60 | -12.46 | -8.06 | -6.94 | 4.31 |
Eisosomes | 0.92 | 0.90 | 0.97 | 0.30 | 0.61 | -2.38 | -5.29 | -2.76 | 0.82 | 2.84 | -0.43 | 0.09 | 0.44 | 1.47 | 1.32 |
Endoplasmic Reticulum | 2.54 | 5.98 | 5.76 | 3.22 | 0.83 | -1.21 | -0.77 | 4.04 | 3.85 | 2.83 | -0.23 | 7.23 | 7.43 | 5.16 | 1.60 |
Endosome | -0.03 | 0.41 | -7.51 | -6.55 | -8.13 | -0.42 | -3.66 | -1.86 | -1.75 | 1.07 | -0.25 | -2.27 | -9.85 | -9.14 | -7.35 |
Golgi | 0.07 | 0.01 | -9.29 | -9.54 | -9.65 | -2.02 | -0.86 | -2.86 | -2.71 | -2.77 | -1.35 | -2.16 | -10.72 | -10.30 | -10.06 |
Lipid Particles | -0.72 | -1.72 | -1.12 | 0.16 | 0.48 | -0.09 | -3.18 | -1.58 | -1.58 | -1.35 | -0.56 | -1.92 | -1.81 | -1.42 | -0.98 |
Mitochondria | -0.67 | -0.57 | -4.48 | -2.37 | -4.47 | -2.94 | 3.23 | 0.36 | 2.52 | -1.26 | -2.78 | -0.53 | -4.84 | -1.49 | -4.61 |
Mitotic Spindle | -1.28 | -12.10 | -8.96 | -8.91 | 2.03 | -1.81 | -1.90 | -3.25 | -3.02 | -2.37 | -1.91 | -12.10 | -9.37 | -8.63 | 1.49 |
None | 1.33 | -4.70 | 0.87 | -2.26 | 6.91 | 0.78 | -2.18 | -1.77 | -2.23 | 1.58 | 1.50 | -6.45 | 0.38 | -2.51 | 7.03 |
Nuclear Periphery | -0.94 | -13.06 | -7.28 | -6.07 | 1.54 | -3.11 | -8.45 | -2.05 | -1.89 | -0.58 | -3.13 | -15.21 | -7.11 | -5.69 | 1.04 |
Nuclear Periphery Foci | -1.93 | -3.97 | -4.44 | -3.88 | 1.99 | -3.67 | -2.71 | -3.14 | -2.93 | 0.36 | -4.11 | -4.60 | -5.43 | -4.50 | 2.08 |
Nucleolus | -2.28 | -9.26 | -3.15 | 0.63 | 7.88 | -3.53 | -3.87 | -1.61 | 0.12 | 2.85 | -4.17 | -10.48 | -4.69 | 1.16 | 8.39 |
Nucleus | -0.32 | -25.65 | -8.52 | -8.05 | 20.56 | -3.16 | -11.02 | -4.11 | -3.50 | 8.42 | -2.91 | -27.95 | -10.16 | -7.51 | 22.07 |
Peroxisomes | -2.16 | -10.43 | -6.80 | -2.26 | 4.85 | 0.36 | -3.84 | -2.86 | -2.90 | 0.60 | -1.95 | -11.17 | -7.58 | -5.63 | 4.88 |
Vacuole | 2.40 | 56.32* | 25.18 | 19.44 | -24.36 | 0.45 | 20.87 | 11.33 | 11.07 | -3.30 | 1.75 | 75.93* | 33.12 | 29.30 | -24.24 |
Vacuole Periphery | -4.56 | 18.46 | 7.35 | 10.18 | -14.37 | -0.71 | 12.39 | 7.69 | 7.73 | -1.49 | -3.47 | 28.14 | 10.91 | 12.50 | -14.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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