Standard name | |
---|---|
Human Ortholog | |
Description | Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 4 | 1 | 3 | 2 | 2 | 3 | 6 | 6 | 4 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 5 | 2 | 7 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 3 | 3 | 0 | 10 | 9 | 17 | 12 | 22 | 15 | 3 | 1 | 0 | 2 | 2 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 1 | 5 | 10 | 6 | 8 | 7 | 2 | 4 | 4 | 7 | 15 | 18 | 17 | 20 | 25 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 5 | 6 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 3 | 11 | 9 | 9 | 9 | 26 | 18 | 12 | 1 | 2 | 7 | 9 | 4 | 16 | 11 | 34 | 37 | 24 | 3 | 9 | 18 | 12 | 21 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 10 | 20 | 9 | 5 | 9 | 38 | 35 | 17 | 59 | 80 | 84 | 43 | 24 | 9 | 9 | 34 | 25 | 46 | 1 | 2 | 3 | 1 | 4 | 4 |
Nucleus | 67 | 93 | 97 | 40 | 48 | 54 | 66 | 58 | 50 | 48 | 53 | 89 | 57 | 38 | 30 | 219 | 127 | 165 | 47 | 83 | 63 | 14 | 21 | 29 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 1 | 5 | 2 | 3 | 3 | 1 | 4 | 4 | 3 | 0 | 0 | 12 | 14 | 15 | 1 | 0 | 2 | 1 | 2 | 7 |
Nucleolus | 107 | 149 | 130 | 117 | 115 | 135 | 185 | 164 | 159 | 170 | 203 | 198 | 119 | 107 | 94 | 268 | 230 | 165 | 93 | 124 | 117 | 58 | 73 | 42 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 3 |
SpindlePole | 6 | 21 | 68 | 47 | 69 | 51 | 52 | 69 | 39 | 38 | 58 | 59 | 16 | 26 | 29 | 83 | 62 | 56 | 15 | 26 | 53 | 25 | 50 | 36 |
Vac/Vac Membrane | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 1 | 5 | 16 | 20 | 18 | 1 | 5 | 6 | 10 | 17 | 24 |
Unique Cell Count | 159 | 230 | 233 | 161 | 189 | 217 | 271 | 238 | 214 | 244 | 286 | 299 | 185 | 167 | 146 | 522 | 405 | 371 | 167 | 259 | 273 | 133 | 208 | 189 |
Labelled Cell Count | 194 | 300 | 320 | 233 | 266 | 321 | 384 | 345 | 340 | 364 | 449 | 434 | 245 | 218 | 199 | 697 | 551 | 521 | 167 | 259 | 273 | 133 | 208 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 7.0 | 6.9 | 6.7 | 7.6 | 5.9 | 6.3 | 6.1 | 5.2 | 4.9 | 5.0 | 5.8 | 7.1 | 7.5 | 7.2 | 9.1 | 10.5 | 8.7 | 6.7 | 6.9 | 6.9 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.6 | 1.7 | 2.3 | 1.7 | 1.6 | 1.6 | 1.3 | 1.2 | 1.3 | 1.5 | 2.2 | 2.2 | 2.6 | 2.8 | 3.1 | 2.8 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.14 | -0.23 | -0.13 | -0.18 | -0.41 | -0.49 | -0.46 | -0.26 | 0.04 | 0.13 | 0.07 | 0.4 | 0.6 | 0.34 | -0.04 | 0.0 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4.4 | 3.4 | 4.3 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 4.8 | 5.0 | 2.6 | 3.5 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.8 | 0.5 | 3.2 | 1.4 | 0.6 | 0 | -2.2 | -0.9 | -0.5 | -1.0 | 2.3 | 1.6 | 1.5 | 2.5 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.4 | 0.5 | 4.7 | 3.6 | 1.6 | 7.0 | 8.1 | 7.5 | 4.1 | 3.4 | 0.7 | 1.0 | 1.5 | 1.3 | 3.5 |
Nucleus | -3.4 | -3.5 | -3.8 | -4.1 | -4.0 | -4.1 | -5.2 | -5.8 | -2.8 | -2.3 | -3.9 | -4.2 | 0.1 | -2.6 | 0.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.3 |
Nucleolus | 3.4 | 1.0 | 1.4 | 2.9 | 2.9 | 4.1 | 3.1 | 3.6 | 2.5 | 1.8 | 1.7 | 1.7 | -1.1 | 0.2 | -2.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1.6 | -1.4 | -2.6 | 0 | -2.7 | -3.6 | -2.4 | -2.5 | -5.2 | -3.2 | -2.0 | -4.2 | -4.2 | -4.2 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 2.1 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.3637 | 6.2664 | 6.0713 | 4.9828 | 5.258 | 5.9918 | 9.7903 | 18.6943 | 8.5981 | 7.4412 | 6.8465 | 7.7922 | 8.3702 | 6.9471 | 7.1107 | 6.4064 | 6.4936 | 6.808 |
Actin | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.003 | 0 | 0.002 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 |
Bud Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0025 | 0.0145 | 0.0076 | 0 | 0 | 0.0088 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.006 | 0 | 0.007 | 0.0001 | 0.0003 | 0.003 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0043 | 0.0005 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0001 |
Mitochondria | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0021 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
None | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.002 | 0 | 0.0049 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0004 | 0.0023 | 0.0008 | 0.0001 | 0.0041 | 0.0009 | 0.0008 | 0.0041 | 0.0012 | 0.0054 | 0.0076 | 0.0012 | 0.0006 | 0.0008 | 0.0009 | 0.0007 | 0.0024 | 0.0001 |
Nucleolus | 0.5292 | 0.4451 | 0.4982 | 0.504 | 0.6251 | 0.6209 | 0.6996 | 0.5304 | 0.6446 | 0.6042 | 0.7232 | 0.6907 | 0.6135 | 0.4229 | 0.5858 | 0.6517 | 0.673 | 0.6881 |
Nucleus | 0.4642 | 0.543 | 0.494 | 0.4082 | 0.2859 | 0.3631 | 0.2654 | 0.4377 | 0.3089 | 0.3797 | 0.2232 | 0.2739 | 0.3674 | 0.5489 | 0.392 | 0.3229 | 0.2642 | 0.2938 |
Peroxisomes | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0055 | 0.0057 | 0.0065 | 0.0872 | 0.0802 | 0.0135 | 0.0157 | 0.0111 | 0.0188 | 0.0099 | 0.0384 | 0.0181 | 0.0172 | 0.0273 | 0.0196 | 0.0244 | 0.0594 | 0.0175 |
Vacuole | 0.0001 | 0.0021 | 0 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Vacuole Periphery | 0.0001 | 0.0009 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.5413 | 25.6202 | 26.9326 | 42.1844 | 47.3774 | 37.2372 | 21.803 | 21.8548 | 38.7872 | 43.0445 |
Translational Efficiency | 1.4994 | 1.3522 | 1.2528 | 1.4139 | 1.2216 | 1.3759 | 1.1397 | 0.9283 | 0.9447 | 1.1524 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2104 | 123 | 1555 | 703 | 1634 | 2130 | 1852 | 1108 | 3738 | 2253 | 3407 | 1811 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 761.86 | 958.88 | 1125.53 | 1172.98 | 779.34 | 890.18 | 1112.77 | 1155.63 | 769.50 | 893.93 | 1118.59 | 1162.36 |
Standard Deviation | 93.99 | 154.39 | 188.52 | 175.45 | 140.68 | 144.48 | 171.84 | 220.02 | 117.04 | 145.87 | 179.75 | 204.05 |
Intensity Change Log 2 | — | 0.331824 | 0.563007 | 0.622581 | — | 0.191844 | 0.513831 | 0.568355 | — | 0.262738 | 0.538349 | 0.595415 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000171 | 0.000784 | 0.000424 | 0.000487 | 0.000275 | 0.000382 | 0.000350 | 0.000474 | 0.000217 | 0.000404 | 0.000384 | 0.000479 |
Bud Neck | 0.004481 | 0.003200 | 0.006741 | 0.011703 | 0.002893 | 0.005650 | 0.007635 | 0.006822 | 0.003787 | 0.005516 | 0.007227 | 0.008717 |
Bud Site | 0.001520 | 0.000730 | 0.000655 | 0.001470 | 0.001201 | 0.001188 | 0.000700 | 0.001570 | 0.001381 | 0.001163 | 0.000679 | 0.001531 |
Cell Periphery | 0.000108 | 0.000049 | 0.000017 | 0.000033 | 0.000091 | 0.000088 | 0.000019 | 0.000036 | 0.000101 | 0.000086 | 0.000018 | 0.000035 |
Cytoplasm | 0.003179 | 0.000303 | 0.000087 | 0.000343 | 0.002618 | 0.001675 | 0.000220 | 0.000262 | 0.002934 | 0.001600 | 0.000159 | 0.000294 |
Cytoplasmic Foci | 0.001926 | 0.000601 | 0.000064 | 0.000245 | 0.001428 | 0.001647 | 0.000051 | 0.000827 | 0.001708 | 0.001590 | 0.000057 | 0.000601 |
Eisosomes | 0.000088 | 0.000082 | 0.000044 | 0.000051 | 0.000064 | 0.000054 | 0.000045 | 0.000046 | 0.000077 | 0.000055 | 0.000045 | 0.000048 |
Endoplasmic Reticulum | 0.001416 | 0.003298 | 0.000588 | 0.000818 | 0.001728 | 0.001801 | 0.000594 | 0.000579 | 0.001552 | 0.001883 | 0.000591 | 0.000672 |
Endosome | 0.001624 | 0.000367 | 0.000056 | 0.000342 | 0.001354 | 0.002229 | 0.000095 | 0.000488 | 0.001506 | 0.002128 | 0.000077 | 0.000432 |
Golgi | 0.002002 | 0.001301 | 0.000319 | 0.000394 | 0.001637 | 0.001304 | 0.000296 | 0.000453 | 0.001843 | 0.001304 | 0.000306 | 0.000430 |
Lipid Particles | 0.000764 | 0.000642 | 0.000019 | 0.000045 | 0.000498 | 0.000465 | 0.000017 | 0.000062 | 0.000648 | 0.000474 | 0.000018 | 0.000056 |
Mitochondria | 0.000984 | 0.000653 | 0.000827 | 0.001587 | 0.000869 | 0.003573 | 0.000801 | 0.001370 | 0.000934 | 0.003413 | 0.000813 | 0.001455 |
Mitotic Spindle | 0.038044 | 0.031202 | 0.021864 | 0.033604 | 0.038023 | 0.023006 | 0.015120 | 0.010801 | 0.038035 | 0.023453 | 0.018198 | 0.019652 |
None | 0.004819 | 0.000901 | 0.000648 | 0.001021 | 0.011703 | 0.002794 | 0.000628 | 0.000863 | 0.007828 | 0.002690 | 0.000637 | 0.000924 |
Nuclear Periphery | 0.000531 | 0.000262 | 0.000157 | 0.000536 | 0.000451 | 0.000715 | 0.000312 | 0.000333 | 0.000496 | 0.000690 | 0.000241 | 0.000412 |
Nuclear Periphery Foci | 0.029024 | 0.011218 | 0.000389 | 0.000899 | 0.019471 | 0.006075 | 0.000468 | 0.000601 | 0.024848 | 0.006356 | 0.000432 | 0.000717 |
Nucleolus | 0.672002* | 0.532717* | 0.537329* | 0.543799* | 0.580200* | 0.522815* | 0.563606* | 0.533023* | 0.631873* | 0.523355* | 0.551613* | 0.537206* |
Nucleus | 0.232473* | 0.400310* | 0.423822* | 0.394660* | 0.330977* | 0.419177* | 0.405176* | 0.433457* | 0.275532* | 0.418147* | 0.413686* | 0.418396* |
Peroxisomes | 0.004203 | 0.010531 | 0.005504 | 0.005804 | 0.003857 | 0.002710 | 0.003430 | 0.006019 | 0.004052 | 0.003137 | 0.004377 | 0.005935 |
Vacuole | 0.000405 | 0.000614 | 0.000214 | 0.001117 | 0.000452 | 0.001669 | 0.000205 | 0.001303 | 0.000426 | 0.001612 | 0.000209 | 0.001231 |
Vacuole Periphery | 0.000237 | 0.000235 | 0.000232 | 0.001044 | 0.000209 | 0.000984 | 0.000230 | 0.000611 | 0.000225 | 0.000943 | 0.000231 | 0.000779 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.40 | -14.13 | -13.11 | 0.71 | -4.57 | -1.57 | -1.77 | -4.04 | -3.59 | -4.53 | -5.27 | -8.78 | -12.11 | -4.52 | -6.08 |
Bud Neck | 1.80 | -6.94 | -9.79 | -10.10 | -6.66 | -5.83 | -10.19 | -9.19 | -4.02 | 1.12 | -4.25 | -11.88 | -12.77 | -8.51 | -4.10 |
Bud Site | 2.61 | 2.64 | -3.10 | -6.09 | -6.36 | 0.53 | 1.59 | -0.62 | -0.89 | -1.31 | 1.52 | 3.06 | -1.60 | -2.52 | -3.25 |
Cell Periphery | 2.30 | 4.98 | 3.41 | 0.51 | -5.87 | 0.19 | 5.38 | 3.44 | 2.86 | -1.96 | 0.74 | 7.02 | 4.69 | 2.94 | -3.68 |
Cytoplasm | 4.41 | 4.84 | 4.11 | -1.13 | -3.33 | 1.70 | 4.12 | 3.06 | 2.07 | -0.94 | 2.46 | 6.28 | 4.75 | 2.65 | -1.57 |
Cytoplasmic Foci | 4.14 | 5.93 | 4.50 | 0.24 | -3.85 | 0.66 | 3.47 | 1.80 | 1.41 | -1.95 | 1.58 | 6.61 | 3.85 | 1.82 | -2.72 |
Eisosomes | 0.89 | 19.42 | 12.53 | 3.22 | -4.04 | 5.01 | 10.34 | 9.21 | 4.32 | -0.21 | 11.82 | 21.86 | 17.04 | 3.55 | -2.92 |
Endoplasmic Reticulum | -2.75 | 11.92 | 2.78 | 3.20 | -3.71 | 0.04 | 11.61 | 11.26 | 10.64 | 0.76 | -2.30 | 16.82 | 10.83 | 9.23 | -2.49 |
Endosome | 5.31 | 7.23 | 5.09 | -0.12 | -2.84 | -1.40 | 4.81 | 2.78 | 3.50 | -1.74 | -0.95 | 8.57 | 5.21 | 3.72 | -2.63 |
Golgi | 2.26 | 8.76 | 7.71 | 2.71 | -6.42 | 1.36 | 4.07 | 3.43 | 3.39 | -0.90 | 3.28 | 8.75 | 7.26 | 3.87 | -1.83 |
Lipid Particles | 0.06 | 3.43 | 3.16 | 1.95 | -3.92 | 0.49 | 5.62 | 4.89 | 3.76 | -1.48 | 1.21 | 4.97 | 4.55 | 4.06 | -2.59 |
Mitochondria | 2.09 | 0.17 | -4.29 | -5.56 | -4.77 | -3.50 | -0.47 | -3.69 | 2.69 | -3.91 | -3.45 | -0.19 | -5.55 | 2.44 | -6.11 |
Mitotic Spindle | 0.35 | 5.29 | -0.08 | -0.37 | -3.72 | 4.18 | 6.41 | 5.54 | 2.19 | -0.24 | 6.21 | 8.61 | 4.40 | -1.06 | -2.75 |
None | 6.60 | 7.27 | 6.42 | -0.76 | -2.80 | 5.24 | 6.68 | 6.50 | 4.10 | -1.95 | 5.92 | 9.11 | 8.64 | 3.99 | -3.19 |
Nuclear Periphery | 4.39 | 7.41 | -1.34 | -3.67 | -4.70 | -2.31 | 1.37 | 0.87 | 2.51 | -0.40 | -1.93 | 4.09 | 0.09 | 1.72 | -2.74 |
Nuclear Periphery Foci | 7.07 | 17.31 | 16.75 | 4.10 | -2.69 | 7.74 | 11.48 | 11.32 | 8.65 | -0.68 | 14.77 | 20.70 | 20.26 | 9.09 | -2.21 |
Nucleolus | 6.51 | 14.74 | 11.24 | -0.04 | 1.11 | 6.55 | 1.76 | 4.74 | -0.19 | 3.27 | 15.34* | 12.29 | 12.45 | -0.27 | 3.14 |
Nucleus | -8.32 | -25.21 | -14.03 | 0.29 | 2.40 | -11.84 | -9.75 | -10.26 | -1.55 | -2.53 | -23.82 | -25.46 | -19.10 | 0.16 | -0.51 |
Peroxisomes | -2.39 | -2.05 | -3.88 | 1.20 | -2.84 | 3.40 | 2.17 | -3.40 | -8.15 | -7.21 | 3.67 | 0.38 | -5.01 | -8.46 | -6.27 |
Vacuole | -1.67 | 0.87 | -3.57 | -2.72 | -3.83 | -3.63 | 2.38 | -1.55 | 0.28 | -1.90 | -3.87 | 2.34 | -2.64 | 0.19 | -2.99 |
Vacuole Periphery | -0.46 | -1.07 | -2.16 | -2.10 | -2.08 | -3.90 | -1.87 | -3.16 | 1.56 | -2.86 | -3.87 | -1.92 | -3.48 | 0.85 | -3.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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