Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.95 0.98 0.97 0.94 0.92 0.87 0.84 0.86 0.73 0.74 1.0 0.99 0.98 – 0.91 0.92 0.74 0.81 0.61 0.87 0.81 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.08 0.08 0 0.15 0.16 0.2 0.28 0.32 0.41 0.43 0 0 0 – 0 0 0.14 0.08 0.21 0 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 3 4 0 2 1
Bud 0 0 3 0 0 2 5 10 5 9 11 7 0 0 3 – 1 0 0 1 2 0 4 4
Bud Neck 1 0 2 1 5 7 7 8 1 4 4 3 0 0 0 – 1 1 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 2 1 1 0 1 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 9 3 1 0 1 1 3 4 2 2 0 3 0 0 3 – 2 1 2 1 4 0 1 0
Cytoplasm 223 147 152 191 307 297 364 448 197 261 183 227 128 271 310 – 74 103 221 123 92 97 166 194
Endoplasmic Reticulum 0 1 0 0 1 2 0 0 0 0 0 0 0 0 1 – 2 11 4 0 0 1 0 1
Endosome 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 – 5 3 2 1 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 1 1 0 0 0 1 0
Mitochondria 2 8 13 15 11 49 64 101 65 98 102 130 0 1 6 – 1 1 42 11 32 3 13 8
Nucleus 1 0 1 0 0 0 2 3 1 0 3 0 0 1 0 – 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 – 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0
SpindlePole 2 0 0 1 1 0 5 8 9 9 6 11 0 0 0 – 3 1 3 2 1 0 2 2
Vac/Vac Membrane 1 0 0 0 0 0 2 2 0 1 1 2 0 0 1 – 3 3 7 2 2 0 4 3
Unique Cell Count 232 153 160 194 315 317 396 513 235 305 251 305 128 273 315 81 112 299 152 152 112 206 226
Labelled Cell Count 240 159 172 208 326 358 457 586 281 384 311 388 128 274 324 93 125 299 152 152 112 206 226


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.2 2.9 2.8 3.2 2.9 3.0 2.9 2.7 2.3 2.9 2.2 3.9 4.1 3.5 4.9 5.3 6.2 3.7 3.7 4.3
Std Deviation (1e-4) 0.8 1.2 1.1 1.2 1.4 1.4 2.1 1.3 1.9 0.8 2.2 1.2 1.5 1.2 1.1 1.4 1.7 3.0 1.3 1.5 1.8
Intensity Change (Log2) – – – -0.06 0.14 -0.02 0.05 -0.01 -0.1 -0.33 0.02 -0.38 0.42 0.5 0.3 0.75 0.88 1.09 0.34 0.38 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1855 0.1569 -0.0332 -0.0331 -0.4548 0.5301 0.0377 0.0619 0.0041 -0.2709 0.2222 0.3585 0.0693 0.1232 -0.4755 -0.0139 -1.2523 -0.2179
Actin 0.0112 0.0001 0.0036 0.0002 0.0001 0.0174 0.0277 0.0002 0.0073 0.001 0.0054 0.0135 0.0489 0.0042 0.0001 0.0001 0.0002 0.0001
Bud 0.0004 0 0.0009 0 0 0.0003 0.0054 0.0002 0.0049 0.0004 0.0004 0.0002 0.0006 0.0002 0 0 0 0
Bud Neck 0.0006 0 0.0003 0 0 0.0006 0.0038 0 0.0021 0 0.0011 0.0005 0.0005 0.0173 0 0 0 0.0001
Bud Periphery 0.0011 0 0.0017 0 0 0.0007 0.0025 0.0004 0.0024 0.0004 0.0004 0.0002 0.0016 0.0005 0 0.0002 0.0001 0
Bud Site 0.0053 0.0001 0.0031 0 0 0.0004 0.0219 0.0007 0.0112 0.0002 0.0012 0.0011 0.0049 0.0104 0 0 0 0
Cell Periphery 0.0007 0.0001 0.0003 0 0 0.0002 0.0004 0.0002 0.0002 0 0.0001 0 0.0004 0.0017 0 0 0.0003 0
Cytoplasm 0.0683 0.0833 0.073 0.0966 0.0444 0.1291 0.0551 0.1178 0.0932 0.0598 0.094 0.1422 0.0019 0.0098 0.0072 0.0095 0.0093 0.0322
Cytoplasmic Foci 0.0116 0.003 0.0082 0.003 0.0018 0.0097 0.03 0.0026 0.0083 0.0309 0.0582 0.0144 0.0027 0.0021 0.0005 0.0012 0.0005 0.001
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0001 0.0002 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0043 0.0039 0.0002 0.0001 0.001 0.0002
Endoplasmic Reticulum 0.0027 0.0008 0.0017 0.0007 0.0005 0.0015 0.0032 0.0008 0.0006 0.0012 0.0007 0.0005 0.0004 0.0003 0.0001 0.0001 0.0002 0.0001
Endosome 0.0175 0.0017 0.0047 0.0006 0.0002 0.0088 0.0331 0.0003 0.0072 0.0204 0.0152 0.0036 0.0021 0.0001 0 0 0 0.0001
Golgi 0.0064 0.0001 0.0021 0 0 0.0085 0.0136 0.0001 0.0032 0.0056 0.0075 0.0046 0.0033 0.0002 0 0 0 0
Lipid Particles 0.0172 0.0002 0.0054 0.0003 0.0001 0.0076 0.0372 0.0002 0.0039 0.0019 0.0279 0.0021 0.0167 0.0002 0.0001 0.0001 0.0001 0
Mitochondria 0.0108 0.0001 0.0101 0.0002 0 0.0069 0.0299 0.0002 0.0008 0.0071 0.0232 0.0008 0.2047 0.0004 0 0.0001 0.0001 0
None 0.796 0.9092 0.8667 0.897 0.9523 0.7733 0.6901 0.8747 0.8443 0.8616 0.7081 0.8105 0.6648 0.9473 0.9914 0.9877 0.9878 0.9658
Nuclear Periphery 0.0153 0.0001 0.0072 0.0002 0.0001 0.0193 0.0081 0.0002 0.0003 0.0011 0.0006 0.0003 0.0003 0.0001 0 0.0001 0 0
Nucleolus 0.0008 0.0001 0.0015 0 0 0.0004 0.001 0.0001 0.0003 0.0014 0.0013 0.0002 0.0004 0.0003 0 0.0001 0.0001 0
Nucleus 0.0191 0.0003 0.0014 0.0002 0.0001 0.0044 0.0009 0.0003 0.0004 0.0005 0.0008 0.0005 0.0002 0.0003 0 0.0001 0 0.0001
Peroxisomes 0.008 0 0.0041 0.0003 0 0.0057 0.0157 0.0001 0.0053 0.0004 0.0482 0.0014 0.0397 0.0004 0 0.0002 0.0001 0
Punctate Nuclear 0.0021 0.0002 0.0025 0.0002 0.0002 0.0014 0.0098 0.0003 0.0015 0.004 0.0031 0.0028 0.0003 0.0002 0.0001 0.0002 0 0
Vacuole 0.0026 0.0003 0.0008 0.0001 0.0001 0.0017 0.0082 0.0004 0.0022 0.0008 0.0021 0.0003 0.0006 0.0001 0 0.0001 0.0001 0.0001
Vacuole Periphery 0.0017 0 0.0008 0 0 0.0019 0.0017 0 0.0003 0.0011 0.0005 0.0001 0.0006 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.0035 48.1316 53.018 68.936 58.0188 56.4431 64.5633 65.2543 61.7235 72.3898
Translational Efficiency 1.0435 1.2087 0.9666 0.7529 0.972 1.1207 0.7378 0.655 0.7916 0.7387

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
107 684 559 1193 1843 1397 1360 59 1950 2081 1919 1252

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 581.79 654.08 931.54 755.57 622.19 654.77 789.51 811.06 619.97 654.54 830.88 758.18
Standard Deviation 79.04 95.51 106.99 105.03 67.94 96.92 92.22 99.64 69.21 96.46 116.30 105.44
Intensity Change Log 2 — 0.168969 0.679119 0.377067 — 0.073633 0.343602 0.382453 — 0.120488 0.515478 0.379853

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000170 0.000969 0.000600 0.001680 0.000111 0.000474 0.000079 0.000349 0.000115 0.000636 0.000231 0.001617
Bud Neck 0.022994 0.048449 0.005415 0.012088 0.018536 0.036900 0.001936 0.002968 0.018781 0.040696 0.002950 0.011658
Bud Site 0.010509 0.022095 0.015464 0.028873 0.003223 0.015305 0.003734 0.011227 0.003623 0.017537 0.007151 0.028041
Cell Periphery 0.000275 0.000184 0.000172 0.000584 0.000099 0.000252 0.000146 0.000343 0.000109 0.000230 0.000153 0.000573
Cytoplasm 0.402354* 0.311484* 0.134400* 0.448727* 0.445678* 0.414191* 0.297670* 0.299887* 0.443301* 0.380432* 0.250110* 0.441713*
Cytoplasmic Foci 0.203790* 0.278358* 0.005739 0.034683 0.239842* 0.239903* 0.014747 0.033487 0.237864* 0.252543* 0.012123 0.034627
Eisosomes 0.000322 0.000207 0.000056 0.000086 0.000165 0.000150 0.000054 0.000101 0.000174 0.000169 0.000054 0.000087
Endoplasmic Reticulum 0.001108 0.000866 0.007903 0.001549 0.000802 0.000600 0.001040 0.004222 0.000819 0.000687 0.003039 0.001675
Endosome 0.003832 0.038070 0.002373 0.013446 0.008769 0.020830 0.001340 0.042258 0.008498 0.026497 0.001641 0.014804
Golgi 0.000707 0.006895 0.000082 0.004464 0.001633 0.006047 0.000042 0.004735 0.001582 0.006326 0.000054 0.004477
Lipid Particles 0.010617 0.015737 0.001747 0.001951 0.011000 0.009781 0.001130 0.003385 0.010979 0.011739 0.001310 0.002018
Mitochondria 0.004808 0.016949 0.000717 0.007257 0.005418 0.016337 0.000301 0.007621 0.005385 0.016538 0.000423 0.007274
Mitotic Spindle 0.000346 0.003802 0.002780 0.041793 0.001341 0.009899 0.003984 0.003483 0.001286 0.007895 0.003634 0.039988
None 0.003059 0.006470 0.003686 0.009462 0.002509 0.004782 0.008077 0.010791 0.002539 0.005337 0.006798 0.009525
Nuclear Periphery 0.000536 0.000663 0.002368 0.001351 0.000625 0.000383 0.001161 0.001736 0.000620 0.000475 0.001512 0.001369
Nuclear Periphery Foci 0.000718 0.000857 0.001447 0.001315 0.000191 0.000271 0.000267 0.001696 0.000220 0.000463 0.000611 0.001333
Nucleolus 0.002724 0.004660 0.000710 0.001240 0.001164 0.001630 0.000771 0.000798 0.001249 0.002626 0.000753 0.001219
Nucleus 0.303860* 0.127183 0.763806* 0.268069* 0.207491* 0.145787* 0.637297* 0.386137* 0.212779* 0.139672* 0.674149* 0.273633*
Peroxisomes 0.004517 0.035537 0.000417 0.001825 0.006708 0.015470 0.000275 0.002167 0.006588 0.022066 0.000317 0.001841
Vacuole 0.022169 0.077339 0.049936 0.113965 0.043939 0.059302 0.025846 0.153373 0.042745 0.065231 0.032863 0.115823
Vacuole Periphery 0.000587 0.003226 0.000181 0.005591 0.000754 0.001706 0.000102 0.029237 0.000745 0.002206 0.000125 0.006706

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.18 -3.84 -1.57 -0.07 -0.51 -2.02 -0.04 -3.99 0.18 -3.99 -3.14 -5.72 -1.76 -0.66 -1.39
Bud Neck -2.27 1.61 1.30 9.35 -2.66 -7.19 12.77 11.94 15.05 -1.43 -9.11 11.64 6.05 14.18 -5.24
Bud Site -1.37 -0.03 -1.10 0.66 -2.34 -7.43 -0.56 -1.34 3.11 -1.05 -9.42 -1.88 -6.82 -0.60 -5.40
Cell Periphery 2.23 2.20 -0.72 -1.79 -1.77 -4.17 -4.54 -3.19 0.46 -2.17 -4.76 -5.41 -2.71 -1.21 -2.19
Cytoplasm 3.38 10.82 0.85 -5.26 -24.37 3.30 18.99 6.33 5.26 0.72 7.50 26.97 6.87 0.61 -14.75
Cytoplasmic Foci -3.77 10.97 9.73 29.39 -12.68 -0.02 44.71 29.48 27.13 -2.90 -2.18 46.64 41.82 44.73 -9.60
Eisosomes 1.19 2.58 2.48 6.88 -1.48 1.41 12.98 3.33 2.32 -4.55 0.44 11.85 8.74 9.49 -5.13
Endoplasmic Reticulum 0.37 -11.66 -1.87 -6.21 13.71 1.12 -6.66 -5.51 -5.72 -4.33 0.83 -15.36 -7.17 -7.92 7.01
Endosome -11.44 0.40 -3.20 9.82 -4.33 -7.70 11.78 -1.05 0.21 -1.84 -12.21 11.31 0.03 10.29 -5.85
Golgi -5.35 3.51 -2.23 3.49 -3.20 -4.91 11.01 -0.16 2.73 -1.46 -6.60 11.10 -0.95 4.29 -3.39
Lipid Particles -1.97 4.61 4.82 7.38 1.60 1.01 12.79 7.80 5.72 -3.85 -0.69 13.00 12.32 11.08 -2.07
Mitochondria -3.22 1.59 0.28 4.52 -3.76 -5.55 6.66 1.01 4.72 -1.35 -6.62 6.70 1.26 7.27 -4.24
Mitotic Spindle -4.05 -1.90 -8.00 -6.90 -6.47 -4.43 -2.24 -1.00 -0.37 -0.72 -4.80 -2.53 -7.73 -5.68 -6.55
None -2.05 0.15 -2.55 0.05 -3.01 -2.46 -4.52 -0.86 -0.15 0.49 -3.42 -4.46 -3.45 -0.75 -0.44
Nuclear Periphery -0.77 -18.12 -11.06 -7.98 5.83 2.95 -9.97 -5.86 -6.73 -3.00 1.62 -15.63 -10.97 -12.80 -0.93
Nuclear Periphery Foci -0.52 -2.73 -0.64 -0.10 4.46 -1.47 -5.87 -3.71 -3.54 -3.31 -2.55 -9.75 -5.09 -2.86 -0.81
Nucleolus -1.41 1.51 1.33 3.94 -1.13 -2.28 1.22 0.65 1.80 -0.15 -3.97 2.01 1.34 4.58 -0.62
Nucleus 6.72 -15.74 -0.11 -17.09 32.44 9.34 -48.99 -5.27 -6.82 5.77 12.17 -59.07 -11.19 -19.75 35.48
Peroxisomes -8.43 4.34 3.56 9.66 -3.09 -6.16 10.76 7.25 10.49 -1.89 -9.79 11.03 9.26 14.23 -4.53
Vacuole -9.22 -9.07 -21.46 -13.83 -11.01 -5.16 -1.07 -5.63 -5.14 -5.50 -8.17 -5.20 -21.88 -18.17 -18.42
Vacuole Periphery -3.81 2.27 -3.00 0.30 -3.59 -6.56 8.99 -1.17 -1.06 -1.24 -5.96 8.95 -3.04 -1.26 -3.80
Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Sin3

Sin3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sin3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available