Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 6 | 6 | 6 | 8 | – | 5 | 11 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 1 |
Bud | 1 | 0 | 3 | 0 | 2 | 0 | 10 | 7 | 10 | – | 20 | 11 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 10 | 15 | 10 | 15 | 16 | 13 | 7 | – | 22 | 14 | 1 | 16 | 13 | 6 | 19 | 14 | 0 | 1 | 0 | 0 | 0 | 1 |
Cytoplasm | 190 | 262 | 527 | 286 | 367 | 366 | 521 | 491 | 267 | – | 298 | 319 | 202 | 473 | 507 | 115 | 187 | 162 | 174 | 248 | 501 | 96 | 284 | 284 |
Endoplasmic Reticulum | 0 | 5 | 0 | 0 | 2 | 7 | 2 | 4 | 10 | – | 5 | 7 | 1 | 6 | 3 | 3 | 3 | 3 | 0 | 1 | 3 | 4 | 6 | 5 |
Endosome | 0 | 1 | 1 | 1 | 1 | 0 | 6 | 7 | 3 | – | 9 | 11 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 1 | 2 | 1 | 2 | 0 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 2 | 0 |
Mitochondria | 2 | 0 | 0 | 3 | 10 | 19 | 56 | 37 | 72 | – | 97 | 73 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 4 | 6 | 0 | 4 | 4 |
Nucleus | 0 | 9 | 0 | 2 | 2 | 1 | 5 | 9 | 1 | – | 3 | 2 | 1 | 4 | 1 | 3 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 3 | 1 | 3 | 8 | 1 | – | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 4 | 1 | 7 | 2 | 13 | 41 | 13 | – | 2 | 25 | 0 | 0 | 2 | 3 | 4 | 10 | 1 | 35 | 4 | 3 | 6 | 7 |
Unique Cell Count | 192 | 275 | 529 | 290 | 384 | 393 | 578 | 574 | 343 | 390 | 422 | 204 | 482 | 514 | 120 | 189 | 174 | 184 | 306 | 533 | 114 | 318 | 321 | |
Labelled Cell Count | 194 | 282 | 547 | 310 | 405 | 419 | 638 | 627 | 393 | 469 | 478 | 207 | 501 | 530 | 134 | 215 | 198 | 184 | 306 | 533 | 114 | 318 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 8.3 | 7.0 | 6.7 | 7.5 | 6.0 | 6.4 | 6.7 | 5.6 | – | 5.3 | 6.0 | 7.6 | 7.9 | 7.8 | 11.7 | 12.8 | 11.0 | 7.1 | 6.9 | 7.8 |
Std Deviation (1e-4) | 1.3 | 1.6 | 1.2 | 1.2 | 1.5 | 1.0 | 1.3 | 1.2 | 1.0 | – | 1.0 | 1.3 | 1.5 | 1.4 | 1.3 | 2.6 | 2.4 | 2.0 | 1.6 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.1 | -0.22 | -0.13 | -0.06 | -0.32 | – | -0.4 | -0.22 | 0.12 | 0.17 | 0.17 | 0.74 | 0.88 | 0.66 | 0.03 | -0.01 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.8 | 0 | 2.8 | – | 4.4 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.6 | 0.7 | 1.8 | 1.0 | 0.4 | 0.2 | – | 3.1 | 1.4 | 0 | 1.4 | 0.7 | 0 | 4.9 | 3.9 |
Cytoplasm | -1.6 | -4.2 | -5.6 | -7.0 | -8.8 | -11.0 | – | -11.5 | -11.7 | -1.0 | -2.3 | -1.7 | -3.6 | -1.1 | -5.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.1 | 7.3 | 5.9 | 11.0 | – | 12.1 | 10.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.0 | 5.4 | 3.2 | – | 0 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0801 | 7.0169 | 5.9538 | 5.4362 | 5.4706 | 5.9781 | 5.2644 | 6.114 | 5.5977 | 4.9969 | 4.5369 | 5.6622 | 3.2896 | 4.1329 | 3.506 | 3.9077 | 3.6847 | 3.5148 |
Actin | 0.0179 | 0.0003 | 0.0016 | 0.011 | 0.0436 | 0.0026 | 0.018 | 0.0018 | 0.0136 | 0.0096 | 0.0025 | 0.0145 | 0.0322 | 0.0006 | 0.0274 | 0.004 | 0.0184 | 0.0072 |
Bud | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0003 | 0.0015 | 0.0015 | 0.0015 | 0.0032 | 0.0012 | 0.001 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0096 | 0.0002 | 0.005 | 0.0005 | 0.0023 | 0.0027 | 0.0096 | 0.0006 | 0.0018 | 0.0049 | 0.0014 | 0.008 | 0.0061 | 0.0002 | 0.0012 | 0.0004 | 0.0007 | 0.001 |
Bud Periphery | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0003 | 0.0017 | 0.0008 | 0.0014 | 0.0033 | 0.0016 | 0.0012 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0054 | 0.0032 | 0.0006 | 0.0006 | 0.0159 | 0.0017 | 0.008 | 0.0087 | 0.0108 | 0.0062 | 0.0034 | 0.0008 | 0.0024 | 0.0013 | 0.0008 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.001 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.8064 | 0.9217 | 0.9337 | 0.8299 | 0.8159 | 0.9156 | 0.8351 | 0.9488 | 0.8877 | 0.8197 | 0.8311 | 0.9004 | 0.6799 | 0.9259 | 0.8807 | 0.7335 | 0.8655 | 0.9082 |
Cytoplasmic Foci | 0.0303 | 0.0116 | 0.0081 | 0.0278 | 0.048 | 0.0196 | 0.0263 | 0.0137 | 0.0222 | 0.0243 | 0.0369 | 0.0249 | 0.0257 | 0.0086 | 0.0091 | 0.0036 | 0.002 | 0.0147 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.004 | 0.0034 | 0.0027 | 0.0024 | 0.0037 | 0.0093 | 0.0022 | 0.0041 | 0.0039 | 0.0045 | 0.004 | 0.012 | 0.0037 | 0.0052 | 0.0144 | 0.003 | 0.0047 |
Endosome | 0.0165 | 0.0083 | 0.0096 | 0.0348 | 0.011 | 0.0043 | 0.035 | 0.0052 | 0.0131 | 0.038 | 0.0377 | 0.0113 | 0.0414 | 0.0063 | 0.0096 | 0.0063 | 0.0067 | 0.0202 |
Golgi | 0.0059 | 0.0007 | 0.002 | 0.0048 | 0.0157 | 0.0007 | 0.0075 | 0.0007 | 0.0045 | 0.0094 | 0.0062 | 0.0032 | 0.0101 | 0.0006 | 0.0027 | 0.0004 | 0.0029 | 0.0015 |
Lipid Particles | 0.0145 | 0.0003 | 0.0052 | 0.0411 | 0.0034 | 0.0002 | 0.0018 | 0.0001 | 0.0012 | 0.0109 | 0.0029 | 0.0004 | 0.0041 | 0.0002 | 0.0027 | 0.0004 | 0.0017 | 0.0005 |
Mitochondria | 0.0063 | 0.0001 | 0.0005 | 0.0032 | 0.002 | 0.0002 | 0.0033 | 0.0004 | 0.0079 | 0.0323 | 0.0036 | 0.0016 | 0.0039 | 0.0002 | 0.0007 | 0.0027 | 0.0012 | 0.0007 |
None | 0.0559 | 0.0469 | 0.025 | 0.0059 | 0.0161 | 0.0443 | 0.0222 | 0.0093 | 0.018 | 0.0137 | 0.0465 | 0.0189 | 0.1413 | 0.0482 | 0.0398 | 0.1229 | 0.0626 | 0.0279 |
Nuclear Periphery | 0.0016 | 0.0003 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0029 | 0.0009 | 0.0009 | 0.0028 | 0.0031 | 0.0008 | 0.0088 | 0.0009 | 0.0028 | 0.0502 | 0.0266 | 0.0083 |
Nucleolus | 0.0014 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0012 | 0 | 0.0003 | 0.001 | 0.0003 | 0.0001 |
Nucleus | 0.0014 | 0.0005 | 0.0007 | 0.0006 | 0.0005 | 0.0006 | 0.0034 | 0.002 | 0.0023 | 0.0018 | 0.0046 | 0.0017 | 0.0059 | 0.0009 | 0.0028 | 0.0526 | 0.0031 | 0.0015 |
Peroxisomes | 0.0094 | 0.0001 | 0.0015 | 0.0328 | 0.0168 | 0.0014 | 0.0062 | 0.0003 | 0.004 | 0.0098 | 0.0039 | 0.0044 | 0.0101 | 0 | 0.0034 | 0.0001 | 0.0003 | 0.0003 |
Punctate Nuclear | 0.0037 | 0.0001 | 0.0003 | 0.0014 | 0.0028 | 0.0002 | 0.0019 | 0.0005 | 0.0014 | 0.0013 | 0.0016 | 0.001 | 0.0075 | 0.0002 | 0.0084 | 0.0032 | 0.0027 | 0.001 |
Vacuole | 0.0032 | 0.0005 | 0.0012 | 0.0008 | 0.0007 | 0.0009 | 0.0044 | 0.0021 | 0.0025 | 0.0032 | 0.0055 | 0.0012 | 0.0033 | 0.0015 | 0.0013 | 0.0016 | 0.0006 | 0.0011 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0008 | 0.0011 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0017 | 0.001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.4614 | 6.2777 | 10.4812 | 21.3128 | 5.6906 | 5.7026 | 13.2897 | 7.6309 | 18.6585 | 12.9505 |
Translational Efficiency | 0.6849 | 0.9667 | 0.8434 | 0.6181 | 1.07 | 1.6998 | 0.6237 | 1.164 | 0.8649 | 0.8441 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1950 | 1589 | 1448 | 1364 | 671 | 1328 | 124 | 2796 | 2621 | 2917 | 1572 | 4160 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 763.21 | 827.90 | 1153.32 | 976.93 | 796.62 | 779.62 | 1129.90 | 1030.11 | 771.76 | 805.92 | 1151.47 | 1012.67 |
Standard Deviation | 99.90 | 119.56 | 189.61 | 147.74 | 113.86 | 114.48 | 155.75 | 139.52 | 104.68 | 119.72 | 187.27 | 144.44 |
Intensity Change Log 2 | — | 0.117376 | 0.595641 | 0.356175 | — | -0.031121 | 0.504231 | 0.370835 | — | 0.043448 | 0.549681 | 0.363681 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000134 | 0.000221 | 0.000175 | 0.001074 | 0.000329 | 0.000356 | 0.000534 | 0.000637 | 0.000184 | 0.000283 | 0.000203 | 0.000780 |
Bud Neck | 0.007455 | 0.010721 | 0.001609 | 0.006686 | 0.006659 | 0.016189 | 0.001749 | 0.008792 | 0.007251 | 0.013210 | 0.001620 | 0.008102 |
Bud Site | 0.008650 | 0.013397 | 0.006182 | 0.042944 | 0.011667 | 0.019821 | 0.005462 | 0.030109 | 0.009422 | 0.016322 | 0.006126 | 0.034318 |
Cell Periphery | 0.000115 | 0.000066 | 0.000090 | 0.000101 | 0.000175 | 0.000075 | 0.000103 | 0.000044 | 0.000130 | 0.000070 | 0.000091 | 0.000063 |
Cytoplasm | 0.701859* | 0.653861* | 0.707004* | 0.659444* | 0.630795* | 0.571968* | 0.425670* | 0.786442* | 0.683666* | 0.616579* | 0.684812* | 0.744802* |
Cytoplasmic Foci | 0.136828 | 0.146556 | 0.015998 | 0.032892 | 0.216599* | 0.157850 | 0.046085 | 0.008703 | 0.157250 | 0.151698 | 0.018371 | 0.016634 |
Eisosomes | 0.000074 | 0.000043 | 0.000014 | 0.000020 | 0.000088 | 0.000064 | 0.000048 | 0.000009 | 0.000078 | 0.000052 | 0.000017 | 0.000012 |
Endoplasmic Reticulum | 0.000575 | 0.000556 | 0.000966 | 0.000724 | 0.001396 | 0.000506 | 0.001684 | 0.000156 | 0.000786 | 0.000534 | 0.001023 | 0.000343 |
Endosome | 0.007506 | 0.011086 | 0.002711 | 0.005807 | 0.015778 | 0.016005 | 0.026533 | 0.002709 | 0.009624 | 0.013325 | 0.004590 | 0.003725 |
Golgi | 0.002553 | 0.002363 | 0.000169 | 0.003073 | 0.003597 | 0.003934 | 0.003139 | 0.001268 | 0.002820 | 0.003078 | 0.000403 | 0.001860 |
Lipid Particles | 0.001532 | 0.001707 | 0.000805 | 0.000998 | 0.003244 | 0.001581 | 0.002308 | 0.000206 | 0.001971 | 0.001650 | 0.000924 | 0.000466 |
Mitochondria | 0.001269 | 0.002794 | 0.000132 | 0.002740 | 0.001124 | 0.006155 | 0.001442 | 0.003107 | 0.001232 | 0.004324 | 0.000235 | 0.002987 |
Mitotic Spindle | 0.000782 | 0.006223 | 0.004590 | 0.053993 | 0.000569 | 0.010384 | 0.014871 | 0.050408 | 0.000728 | 0.008117 | 0.005401 | 0.051584 |
None | 0.004550 | 0.002920 | 0.007223 | 0.003835 | 0.002233 | 0.001514 | 0.004019 | 0.003749 | 0.003957 | 0.002280 | 0.006970 | 0.003777 |
Nuclear Periphery | 0.000071 | 0.000175 | 0.000395 | 0.000250 | 0.000105 | 0.000234 | 0.000972 | 0.000308 | 0.000080 | 0.000202 | 0.000440 | 0.000289 |
Nuclear Periphery Foci | 0.000563 | 0.000673 | 0.002743 | 0.001417 | 0.001601 | 0.000380 | 0.005656 | 0.000247 | 0.000829 | 0.000539 | 0.002973 | 0.000631 |
Nucleolus | 0.000460 | 0.000599 | 0.000246 | 0.000219 | 0.000332 | 0.000859 | 0.000152 | 0.000202 | 0.000427 | 0.000717 | 0.000239 | 0.000207 |
Nucleus | 0.044032 | 0.064779 | 0.159034* | 0.041906 | 0.036673 | 0.065814 | 0.042077 | 0.052012 | 0.042148 | 0.065250 | 0.149808* | 0.048699 |
Peroxisomes | 0.001292 | 0.003917 | 0.000190 | 0.003965 | 0.001657 | 0.008321 | 0.001404 | 0.002578 | 0.001386 | 0.005922 | 0.000286 | 0.003033 |
Vacuole | 0.078916 | 0.076388 | 0.089526 | 0.135869 | 0.064137 | 0.115369 | 0.415531* | 0.046127 | 0.075132 | 0.094134 | 0.115242 | 0.075552 |
Vacuole Periphery | 0.000781 | 0.000956 | 0.000196 | 0.002041 | 0.001243 | 0.002622 | 0.000562 | 0.002185 | 0.000900 | 0.001714 | 0.000224 | 0.002138 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.37 | -5.21 | -5.43 | -4.42 | -4.42 | -0.17 | 0.38 | 0.10 | 0.37 | -0.33 | -3.42 | -1.59 | -4.36 | -2.73 | -3.82 |
Bud Neck | -3.05 | 9.00 | 5.27 | 7.41 | -3.53 | -5.61 | 3.47 | 1.79 | 9.47 | -3.19 | -6.64 | 9.46 | 4.79 | 11.42 | -5.19 |
Bud Site | -3.14 | 2.91 | -5.63 | -3.01 | -6.67 | -3.44 | 2.88 | -0.57 | 3.24 | -4.05 | -5.52 | 3.89 | -5.26 | 0.40 | -7.63 |
Cell Periphery | 7.41 | 2.88 | 4.40 | -2.79 | 1.32 | 9.25 | 5.59 | 13.21 | 7.65 | 6.16 | 10.70 | 5.26 | 14.17 | 5.17 | 6.94 |
Cytoplasm | 6.14 | 6.92 | 14.27 | 7.89 | 7.27 | 5.64 | 11.28 | -7.30 | -15.89 | -15.33 | 10.69 | 7.76 | 3.49 | -7.84 | -5.24 |
Cytoplasmic Foci | -1.37 | 34.16 | 29.24 | 25.37 | -7.31 | 7.29 | 24.79 | 29.62 | 30.54 | 4.02 | 1.75 | 42.69 | 43.58 | 40.91 | 0.13 |
Eisosomes | 7.21 | 15.12 | 12.39 | 7.85 | -8.03 | 4.02 | 6.71 | 14.53 | 17.00 | 10.03 | 6.96 | 18.53 | 19.21 | 19.63 | 0.62 |
Endoplasmic Reticulum | 0.01 | -2.72 | 0.63 | 0.76 | 4.10 | 2.40 | -0.34 | 3.36 | 6.22 | 6.52 | 1.82 | -1.35 | 4.08 | 5.01 | 8.60 |
Endosome | -3.71 | 11.72 | 8.91 | 9.38 | -0.85 | 0.31 | 2.82 | 9.52 | 12.47 | 2.17 | -3.83 | 12.27 | 15.04 | 16.20 | 3.12 |
Golgi | 0.75 | 8.90 | 3.51 | 3.07 | -1.68 | -0.00 | 4.52 | 5.62 | 4.81 | 0.32 | -0.17 | 10.59 | 7.94 | 6.53 | -2.37 |
Lipid Particles | -0.85 | 5.05 | 3.18 | 2.62 | -1.74 | 4.91 | 3.65 | 8.61 | 9.68 | 7.29 | 1.79 | 7.50 | 11.16 | 7.27 | 4.05 |
Mitochondria | -1.73 | 2.93 | 0.66 | 2.04 | -1.23 | -3.59 | 2.47 | -0.08 | 3.64 | -3.52 | -3.78 | 3.65 | 0.49 | 4.16 | -4.11 |
Mitotic Spindle | -3.50 | -5.82 | -6.98 | -4.62 | -4.22 | -5.24 | -3.03 | -10.19 | -4.15 | -3.97 | -6.08 | -6.41 | -12.20 | -6.62 | -6.74 |
None | 2.75 | -1.96 | 3.60 | 0.55 | 6.31 | 1.14 | -0.11 | -3.39 | -8.29 | -2.60 | 3.76 | -2.88 | 1.06 | -4.39 | 4.80 |
Nuclear Periphery | -7.55 | -21.70 | -20.08 | -12.19 | 4.97 | -6.39 | -10.40 | -10.25 | -6.10 | 6.54 | -10.44 | -23.44 | -15.65 | -9.89 | 5.25 |
Nuclear Periphery Foci | -1.46 | -10.13 | -6.97 | -4.98 | 6.11 | 7.17 | -5.40 | 7.79 | 2.06 | 7.72 | 4.11 | -10.08 | 2.86 | -1.62 | 11.01 |
Nucleolus | -1.39 | 4.19 | 4.74 | 5.12 | 1.10 | -1.27 | 1.51 | 1.24 | 3.57 | -2.30 | -2.13 | 3.51 | 3.62 | 5.43 | 0.16 |
Nucleus | -8.71 | -25.94 | -9.07 | -0.13 | 20.16 | -8.33 | -4.10 | -18.32 | -5.95 | -1.65 | -11.58 | -26.37 | -21.27 | -6.09 | 17.78 |
Peroxisomes | -6.30 | 11.70 | -0.41 | 4.40 | -3.43 | -7.23 | 5.30 | 2.26 | 7.87 | -1.22 | -9.58 | 13.50 | 1.41 | 9.22 | -3.29 |
Vacuole | -0.98 | -9.32 | -25.45 | -24.36 | -15.46 | -11.67 | -17.42 | -15.95 | -2.03 | 14.24 | -8.75 | -14.09 | -27.27 | -18.35 | -3.37 |
Vacuole Periphery | -1.48 | 9.45 | 1.00 | 1.68 | -2.10 | -1.45 | 4.99 | 2.02 | 2.25 | -1.88 | -1.97 | 10.13 | 1.57 | 2.56 | -2.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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