Standard name
Human Ortholog
Description Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; Ahc2p may tether Ahc1p to the complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.97 0.95 0.94 0.9 0.81 0.85 0.66 0.68 0.43 0.51 0.92 0.95 0.98 0.85 0.81 0.76 0.73 0.79 0.59 0.86 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.12 0.17 0.11 0.45 0.29 0.65 0.5 0 0 0 0 0 0.05 0.14 0.06 0.18 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0.13 0.15 0.15 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 3 2 2 0 0 0
Bud 0 0 0 0 1 0 0 3 1 0 7 2 0 0 0 0 1 0 0 3 0 0 4 0
Bud Neck 0 1 4 3 0 1 6 9 2 3 1 6 1 0 1 2 0 1 0 3 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 1 0 0 1 1 0 0 0 1 1 0 1 1 1 1 0 0 1 0 0 0
Cytoplasm 204 241 107 168 151 177 214 236 114 137 103 108 68 35 123 163 126 148 174 226 74 28 57 62
Endoplasmic Reticulum 0 1 1 1 1 0 0 1 0 1 0 2 0 1 2 11 9 7 4 1 2 1 0 0
Endosome 0 0 0 1 0 0 6 0 1 1 0 0 1 0 0 1 2 10 1 3 4 0 0 1
Golgi 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Mitochondria 13 1 0 4 2 23 45 31 79 59 156 107 0 0 0 7 3 9 33 18 23 0 1 1
Nucleus 0 0 2 1 3 5 5 7 4 12 8 10 1 1 0 3 4 4 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 0 1 0 1 5 6 3 4 1 12 0 0 0 0 2 3 4 7 1 0 1 0
Vac/Vac Membrane 6 11 0 1 6 5 7 8 3 10 7 7 5 1 1 24 23 30 9 12 8 0 0 0
Unique Cell Count 217 252 110 176 160 197 265 279 174 202 239 212 74 37 125 191 155 195 239 286 126 33 67 72
Labelled Cell Count 226 256 116 181 165 213 289 303 209 228 284 256 77 38 128 212 173 217 239 286 126 33 67 72


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 4.8 4.6 5.4 4.2 4.4 4.6 3.6 3.8 3.4 3.6 5.1 5.4 4.9 5.5 5.5 5.4 – 4.8 5.4
Std Deviation (1e-4) 0.7 1.0 0.8 1.0 2.0 1.3 1.5 1.8 1.2 1.0 1.3 1.3 1.4 2.8 1.6 0.9 1.2 1.6 – 1.6 2.0
Intensity Change (Log2) – – – -0.06 0.17 -0.21 -0.14 -0.06 -0.42 -0.34 -0.5 -0.42 0.08 0.15 0.01 0.19 0.18 0.17 – 0.0 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 4.2 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2116 -0.855 -0.783 -0.6782 -0.4697 -0.6685 -0.6585 -0.4956 -0.3015 -0.6261 0.0745 -0.1995 -0.3074 -1.1751 -1.3279 -1.3965 -0.6304 -1.2523
Actin 0.0549 0.0005 0.0108 0.0018 0.0091 0.0053 0.0411 0.0002 0.0182 0.0589 0.0193 0.0161 0.051 0.0004 0.0077 0.0143 0.0802 0.0066
Bud 0.0011 0.0028 0.0003 0.0001 0.0099 0.0002 0.0005 0.0001 0.0008 0.0007 0.0038 0.0003 0.0021 0.0004 0.0002 0.0001 0.0003 0.005
Bud Neck 0.0013 0.0002 0.0005 0.0007 0.026 0.0025 0.0036 0.0001 0.0053 0.0025 0.0021 0.0024 0.0061 0.0003 0.0007 0.0009 0.0016 0.0037
Bud Periphery 0.0021 0.0005 0.0004 0.0002 0.0225 0.0005 0.0008 0.0001 0.0013 0.0009 0.0091 0.0003 0.0039 0.0009 0.0002 0.0001 0.0006 0.0032
Bud Site 0.0036 0.0017 0.0055 0.0004 0.0201 0.0003 0.0153 0.0004 0.0049 0.0088 0.0079 0.0003 0.0122 0.0012 0.0022 0.0004 0.0012 0.0019
Cell Periphery 0.0005 0.0001 0.0001 0.0001 0.0014 0.0001 0.0004 0.0001 0.0005 0.0003 0.0015 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0651 0.1304 0.1253 0.0645 0.1416 0.1847 0.082 0.1039 0.0518 0.0797 0.0515 0.1922 0.1236 0.1851 0.2154 0.1913 0.1431 0.2828
Cytoplasmic Foci 0.0361 0.0141 0.0208 0.0328 0.0127 0.0114 0.0093 0.0025 0.0168 0.0123 0.0154 0.0062 0.0621 0.0118 0.0123 0.0115 0.007 0.0118
Eisosomes 0.0009 0.0002 0.0004 0.0001 0.0008 0.0002 0.0006 0.0003 0.0004 0.0004 0.0006 0.0001 0.0007 0.0001 0.0001 0.0001 0.001 0.0001
Endoplasmic Reticulum 0.0097 0.0029 0.0026 0.002 0.0049 0.003 0.0037 0.0013 0.0067 0.0014 0.0035 0.0024 0.0083 0.0019 0.0031 0.0023 0.0023 0.0025
Endosome 0.0519 0.0036 0.0133 0.0447 0.0488 0.009 0.0289 0.001 0.0282 0.0042 0.0197 0.006 0.0322 0.0031 0.009 0.0047 0.0044 0.0047
Golgi 0.0251 0.0002 0.009 0.0135 0.022 0.0025 0.0077 0.0001 0.0073 0.0022 0.0072 0.0065 0.0106 0.0002 0.0016 0.0038 0.0032 0.0017
Lipid Particles 0.0257 0.0019 0.0208 0.0397 0.0036 0.0035 0.017 0.0002 0.0053 0.0014 0.018 0.0019 0.02 0.0009 0.0037 0.0176 0.003 0.0025
Mitochondria 0.025 0.0004 0.0186 0.0216 0.1085 0.0033 0.0124 0.0005 0.0048 0.0012 0.0367 0.0082 0.0045 0.0012 0.0019 0.0049 0.0026 0.0011
None 0.5971 0.7643 0.6449 0.6753 0.3587 0.5054 0.6544 0.8153 0.7618 0.6767 0.6059 0.547 0.4989 0.688 0.5992 0.5338 0.6056 0.3712
Nuclear Periphery 0.0184 0.0065 0.0084 0.0061 0.0095 0.0126 0.0117 0.0021 0.0059 0.0027 0.0143 0.0066 0.0174 0.0032 0.0083 0.0075 0.0062 0.0077
Nucleolus 0.0025 0.0012 0.0012 0.0017 0.008 0.0042 0.0026 0.0016 0.0009 0.0019 0.0258 0.0026 0.0027 0.0014 0.0017 0.0013 0.0017 0.0046
Nucleus 0.0388 0.0417 0.0814 0.0521 0.1245 0.2103 0.0718 0.0553 0.0469 0.0744 0.0435 0.1775 0.0791 0.0704 0.1058 0.1552 0.1204 0.2282
Peroxisomes 0.0124 0.0007 0.0093 0.0048 0.0014 0.002 0.0146 0.0001 0.0113 0.0015 0.0771 0.0008 0.0242 0.0043 0.0014 0.0269 0.0012 0.001
Punctate Nuclear 0.0195 0.0247 0.0237 0.0321 0.0552 0.0345 0.0104 0.0134 0.016 0.0671 0.0074 0.0206 0.0347 0.0225 0.0227 0.0218 0.013 0.0568
Vacuole 0.0051 0.0013 0.0016 0.0042 0.0084 0.0031 0.0069 0.0015 0.0032 0.0007 0.0232 0.0014 0.0038 0.0025 0.002 0.0011 0.001 0.0023
Vacuole Periphery 0.0034 0.0002 0.001 0.0015 0.0023 0.0013 0.004 0.0001 0.0015 0.0001 0.0065 0.0005 0.0014 0.0002 0.0005 0.0003 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1355 15.0769 17.6421 18.2993 13.0361 10.3731 20.6676 22.952 16.3186 15.7261
Translational Efficiency 1.272 0.9163 0.9213 0.5698 0.6184 1.0472 0.7615 0.8872 1.01 0.7312

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
797 197 1155 1142 1272 1520 152 137 2069 1717 1307 1279

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 580.30 651.33 868.25 832.77 651.85 682.22 891.70 876.85 624.29 678.68 870.98 837.49
Standard Deviation 58.82 82.48 102.53 112.14 70.53 89.62 84.71 88.68 74.85 89.38 100.90 110.71
Intensity Change Log 2 — 0.166590 0.581312 0.521119 — 0.065697 0.452018 0.427790 — 0.114094 0.514357 0.472498

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000649 0.000319 0.000699 0.000059 0.000207 0.000170 0.000684 0.000065 0.000258 0.000301 0.000697
Bud Neck 0.015630 0.047170 0.003265 0.006592 0.013039 0.027925 0.000882 0.004359 0.014037 0.030133 0.002988 0.006353
Bud Site 0.002644 0.018569 0.011421 0.015523 0.002363 0.010082 0.001022 0.004884 0.002471 0.011055 0.010212 0.014383
Cell Periphery 0.000298 0.000217 0.000143 0.000481 0.000185 0.000134 0.000266 0.000178 0.000229 0.000143 0.000157 0.000448
Cytoplasm 0.396478* 0.239526* 0.224350* 0.165044* 0.393398* 0.341210* 0.291665* 0.279689* 0.394584* 0.329543* 0.232179* 0.177324*
Cytoplasmic Foci 0.239928* 0.287742* 0.007556 0.005538 0.258262* 0.176164 0.051600 0.001737 0.251199* 0.188966* 0.012678 0.005131
Eisosomes 0.000300 0.000226 0.000042 0.000041 0.000201 0.000109 0.000152 0.000051 0.000239 0.000122 0.000055 0.000042
Endoplasmic Reticulum 0.000784 0.001056 0.004626 0.006717 0.001079 0.001043 0.002944 0.006901 0.000965 0.001044 0.004431 0.006737
Endosome 0.006223 0.039201 0.002065 0.003039 0.011027 0.018630 0.006056 0.001287 0.009176 0.020990 0.002529 0.002851
Golgi 0.001786 0.006825 0.000074 0.001344 0.002573 0.004613 0.000109 0.000070 0.002270 0.004867 0.000078 0.001207
Lipid Particles 0.023120 0.024356 0.001797 0.001075 0.022084 0.013016 0.005999 0.001068 0.022483 0.014317 0.002286 0.001074
Mitochondria 0.007720 0.019177 0.000716 0.001967 0.007116 0.020406 0.000253 0.000885 0.007349 0.020265 0.000662 0.001851
Mitotic Spindle 0.000097 0.006744 0.002642 0.024030 0.001703 0.012815 0.000186 0.009924 0.001085 0.012118 0.002356 0.022519
None 0.007691 0.005265 0.004646 0.009647 0.006505 0.006760 0.005424 0.010636 0.006962 0.006589 0.004736 0.009753
Nuclear Periphery 0.000940 0.000797 0.002386 0.002802 0.001010 0.001792 0.001491 0.001848 0.000983 0.001678 0.002282 0.002700
Nuclear Periphery Foci 0.000258 0.001011 0.001185 0.001695 0.000290 0.000999 0.001881 0.000709 0.000278 0.001000 0.001266 0.001589
Nucleolus 0.001968 0.008625 0.000908 0.001238 0.002060 0.006434 0.002170 0.000609 0.002025 0.006686 0.001055 0.001171
Nucleus 0.264449* 0.170844* 0.698467* 0.713626* 0.239352* 0.292156* 0.530738* 0.616913* 0.249020* 0.278237* 0.678960* 0.703267*
Peroxisomes 0.007515 0.050464 0.000236 0.000305 0.008856 0.014826 0.000673 0.000137 0.008339 0.018915 0.000287 0.000287
Vacuole 0.021206 0.068314 0.032885 0.037553 0.027976 0.047800 0.096091 0.057286 0.025368 0.050153 0.040235 0.039666
Vacuole Periphery 0.000890 0.003223 0.000270 0.001045 0.000861 0.002881 0.000229 0.000144 0.000872 0.002920 0.000266 0.000949

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.93 -9.94 -12.52 -0.96 -6.08 -3.94 -5.34 -6.07 -5.05 -4.38 -5.08 -13.08 -14.78 -9.20 -7.17
Bud Neck -4.14 7.11 4.07 5.22 -7.94 -6.85 9.86 1.62 7.71 -6.00 -7.81 10.33 4.79 11.38 -9.39
Bud Site -4.13 -3.12 -4.83 1.74 -0.60 -4.87 0.90 -2.75 1.85 -3.65 -6.09 -3.13 -5.16 0.23 -0.85
Cell Periphery 1.87 4.93 0.37 -0.68 -1.68 4.81 -4.87 0.57 -1.24 3.39 6.07 4.23 -0.48 -1.86 -1.47
Cytoplasm 7.75 19.08 27.59 6.14 8.09 5.40 6.62 14.56 11.71 5.14 7.77 23.50 37.01 24.76 9.43
Cytoplasmic Foci -2.57 31.05 31.56 17.78 4.27 10.32 26.80 41.73 33.87 8.61 9.13 49.83 52.16 37.47 8.16
Eisosomes 2.76 12.92 12.55 9.85 -2.09 9.33 1.20 8.10 0.74 5.29 11.48 17.61 18.34 11.10 0.73
Endoplasmic Reticulum -1.08 -17.56 -21.33 -19.02 -6.86 0.11 -6.25 -9.72 -9.82 -6.21 -0.48 -17.41 -22.53 -22.70 -8.44
Endosome -6.41 5.56 5.86 7.26 1.18 -4.96 3.58 9.70 12.99 6.95 -8.46 9.73 10.79 14.68 2.95
Golgi -1.75 6.94 3.70 2.22 -1.82 -2.18 5.00 4.99 5.88 -0.07 -3.11 6.89 4.68 5.60 -1.82
Lipid Particles -0.09 10.37 10.76 3.95 4.16 5.52 9.85 14.20 13.47 6.72 5.37 16.92 18.12 13.42 6.67
Mitochondria -1.89 4.39 3.81 3.13 -5.52 -6.55 6.38 5.46 11.27 -4.72 -6.84 7.38 6.30 11.40 -6.16
Mitotic Spindle -2.88 -3.28 -6.46 -3.31 -5.01 -5.48 2.11 -1.92 -0.56 -2.12 -6.31 -1.89 -6.33 -2.03 -5.43
None 1.01 3.48 0.96 -0.42 -3.62 -0.28 1.43 -0.18 0.03 -1.35 0.14 3.15 0.35 0.21 -3.86
Nuclear Periphery 0.44 -7.25 -10.63 -14.62 -6.04 -3.83 -5.13 -6.74 -5.14 -4.73 -3.49 -11.99 -15.56 -11.52 -7.13
Nuclear Periphery Foci -2.57 -7.28 -7.30 -2.56 -0.18 -1.89 -6.93 -3.93 -1.90 0.88 -2.18 -8.41 -7.96 -2.56 0.03
Nucleolus -3.07 3.55 2.66 3.46 -0.87 -6.51 0.90 5.53 8.27 1.88 -7.25 5.70 4.46 8.60 -0.26
Nucleus 5.46 -40.30 -38.45 -30.94 0.37 -5.63 -11.18 -14.52 -12.51 -3.76 -3.59 -48.01 -47.49 -40.44 -1.08
Peroxisomes -5.82 7.33 7.38 6.88 1.64 -3.92 9.67 10.31 11.60 5.73 -6.76 12.51 12.67 13.19 3.41
Vacuole -5.96 -8.54 -13.41 -2.69 -6.96 -7.93 -8.34 -6.99 -5.94 -0.49 -10.46 -10.84 -14.73 -9.20 -6.15
Vacuole Periphery -3.45 3.79 1.84 4.10 -1.22 -4.25 9.94 10.74 5.78 2.21 -4.74 6.94 2.65 5.51 -1.17
Standard name
Human Ortholog
Description Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; Ahc2p may tether Ahc1p to the complex
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ahc1

Ahc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ahc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available