Standard name
Description Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.2 0.43 0.34 0.32 0.36 0.33 0.34 0.29 0.34 0.22 0.31 0.44 0.46 0.54 0.21 0.19 0.12 0.18 0.09 0.16 0.22 0.24 0.25
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.09 0.09 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0 0 0.07 0.09 0.39 0.54 0.44 0.65 0.54 0.73 0.55 0 0 0 0.09 0 0 0.05 0 0.06 0 0.06 0
Nucleus 0.31 0.46 0.34 0.41 0.34 0.28 0.2 0.31 0.22 0.21 0.28 0.28 0.42 0.27 0.27 0.38 0.55 0.54 0.39 0.51 0.32 0.33 0.28 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.19 0.14 0.06 0.06 0.06 0 0.05 0 0.05 0.05 0.12 0.07 0.16 0.06 0.09 0.1 0.07 0.13 0 0 0.06
Vac/Vac Membrane 0.21 0.4 0.06 0.07 0.16 0.1 0.07 0.06 0.06 0.09 0 0.07 0.06 0.12 0.09 0.2 0.17 0.2 0.22 0.2 0.25 0.3 0.29 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 4
Bud 0 0 0 0 0 0 4 3 4 2 8 6 0 0 0 0 0 0 2 4 1 4 13 9
Bud Neck 0 0 3 2 8 1 4 8 2 1 0 3 5 8 5 0 0 0 0 1 0 1 9 13
Bud Site 0 0 0 0 0 2 5 8 4 6 7 6 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 41 32 42 46 68 84 95 118 64 79 70 73 116 180 234 27 34 24 38 17 24 59 118 123
Endoplasmic Reticulum 2 0 1 0 2 1 1 0 1 0 0 1 0 3 2 10 5 9 0 0 0 0 2 3
Endosome 3 7 3 6 7 4 0 2 2 2 0 1 7 32 32 12 16 21 3 4 1 1 5 6
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 1 1 2 1 0 1 0 1 0 0 1 1
Mitochondria 71 4 3 10 19 92 156 155 144 124 227 130 9 0 3 12 5 8 9 5 9 11 27 20
Nucleus 51 73 33 55 71 67 58 109 50 49 87 65 110 107 117 50 97 103 83 98 49 88 136 110
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 4 1 1 2 1 1 1 1 2 3 2 1 4 2 2 11 12 22 0 1 1 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 7 3 10 25 30 15 17 20 5 12 2 11 14 47 30 21 11 17 20 14 21 9 20 31
Vac/Vac Membrane 35 63 6 10 33 23 20 20 13 20 14 16 15 47 38 26 30 39 45 39 39 82 142 151
Unique Cell Count 164 159 98 134 210 236 287 350 223 231 312 236 263 392 433 130 176 192 211 193 158 270 498 493
Labelled Cell Count 214 183 102 156 239 290 362 445 292 299 417 314 285 428 467 170 210 245 211 193 158 270 498 493


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 5.0 5.2 5.1 4.8 3.9 3.6 4.1 3.8 3.8 3.2 3.7 4.8 5.4 5.3 5.8 6.6 6.4 4.9 5.3 5.5
Std Deviation (1e-4) 0.9 1.3 1.6 2.5 1.3 1.6 1.3 1.5 2.8 1.8 0.7 0.8 1.1 1.4 1.3 1.5 1.8 1.5 1.7 1.8 2.1
Intensity Change (Log2) – – – -0.03 -0.11 -0.43 -0.52 -0.33 -0.45 -0.44 -0.71 -0.49 -0.11 0.05 0.04 0.18 0.36 0.3 -0.09 0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.8 -1.2 -1.7 -1.7 -2.5 -1.5 -4.0 -2.1 0.2 0.5 2.0 -3.6 -4.2 -5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.8 1.6 1.9 1.9 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Nucleus 1.1 0 -1.0 -2.7 -0.5 -2.1 -2.4 -1.1 -1.1 1.4 -1.2 -1.3 0.7 3.4 3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.8 0 0 0 0 0 0 0 0 0 0.5 -1.1 1.3 -1.2 -0.4
Vacuole 0.4 2.4 1.1 0.3 -0.2 -0.1 0.8 0 0.2 -0.2 1.7 0.9 3.0 2.6 3.2