Standard name
Human Ortholog
Description 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.75 0.73 0.91 0.84 0.78 0.83 0.85 0.82 0.67 0.61 0.51 0.65 0.59 0.62 0.66 0.47 – – 0.52 0.53 0.54 0.62 0.76 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.15 0.08 0 0 0 0.13 0.13 0.08 0.4 0.45 0.53 0.27 0.08 0 0 0.09 – – 0.09 0.06 0.07 0.14 0.09 0.08
Nucleus 0.09 0.06 0.05 0.1 0.17 0.05 0 0.07 0.08 0.11 0.16 0.13 0.25 0.22 0.19 0.11 – – 0.11 0.15 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0.05 0.05 0 0 0
Vac/Vac Membrane 0.1 0.19 0 0 0 0 0 0 0 0 0 0 0.13 0.19 0.19 0.35 – – 0.14 0.15 0.15 0.1 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 1 – – 1 2 3 0 2 3
Bud 0 1 0 1 0 3 2 2 0 7 8 4 2 0 0 0 – – 2 4 0 1 1 5
Bud Neck 1 1 14 6 12 13 14 27 4 9 3 5 0 0 0 0 – – 1 1 1 1 1 2
Bud Site 0 0 0 0 0 0 0 0 2 2 5 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 1 5 0 7 0 0 1 0 1 0 0 0 0 0 1 – – 0 0 2 0 1 2
Cytoplasm 211 257 229 184 256 336 419 346 187 178 142 132 136 142 184 26 – – 174 214 158 87 162 186
Endoplasmic Reticulum 2 2 2 0 1 0 2 0 1 1 0 2 1 0 0 2 – – 2 0 1 1 1 3
Endosome 0 2 0 0 0 0 0 1 0 0 0 0 0 1 2 0 – – 4 4 3 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 – – 0 0 0 0 1 0
Mitochondria 41 27 0 8 5 53 63 32 113 132 147 55 19 4 8 5 – – 28 23 21 20 19 20
Nucleus 26 22 12 22 55 22 18 31 23 31 45 26 57 50 53 6 – – 36 59 20 4 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 0 1 0 0 1 1 0 0 – – 1 0 0 0 1 0
Nucleolus 0 0 0 0 1 1 0 0 0 1 0 0 1 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 4 1 11 5 1 2 2 5 0 0 0 0 – – 17 20 14 1 1 4
Vac/Vac Membrane 28 66 7 3 7 3 5 9 3 4 5 1 30 43 52 19 – – 46 61 45 14 14 22
Unique Cell Count 280 353 253 218 327 406 491 420 280 293 278 202 232 228 279 55 333 406 294 141 214 260
Labelled Cell Count 309 379 269 224 349 432 534 455 334 369 357 232 247 242 299 61 333 406 294 141 214 260


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 4.6 4.0 4.5 3.6 3.7 3.9 3.0 3.0 2.9 3.4 4.1 4.4 4.3 6.2 – – 3.8 3.8 4.2
Std Deviation (1e-4) 0.5 0.7 0.6 0.7 1.1 1.0 1.0 0.9 0.4 0.7 0.8 0.8 0.8 1.3 1.0 1.5 – – 1.6 1.7 1.6
Intensity Change (Log2) – – – -0.21 -0.02 -0.36 -0.3 -0.22 -0.6 -0.63 -0.63 -0.42 -0.17 -0.06 -0.09 0.45 – – -0.26 -0.27 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3489 -1.0145 -0.9287 -1.411 -1.3663 -1.0746 -1.3506 -0.9261 -0.874 -0.8237 -1.2071 -1.16 -1.1829 -0.8998 -1.0019 -1.0188 -1.2616 -1.3694
Actin 0.0155 0.0003 0.0057 0.0031 0.0233 0.0065 0.024 0.0002 0.0085 0.0145 0.0031 0.0006 0.0473 0.0002 0.01 0.0012 0.0391 0.0073
Bud 0.0029 0.0006 0.0011 0.0007 0.0002 0.0034 0.0016 0.0003 0.0002 0.0004 0.0011 0.0002 0.0006 0.0001 0.0002 0.0001 0.0006 0.0002
Bud Neck 0.0012 0.0004 0.0016 0.0006 0.0021 0.002 0.0014 0.0004 0.0006 0.0007 0.0008 0.0018 0.0092 0.0002 0.0009 0.001 0.0003 0.0011
Bud Periphery 0.0067 0.0003 0.0018 0.0021 0.0003 0.0013 0.0037 0.0003 0.0003 0.0004 0.0015 0.0002 0.0008 0.0001 0.0003 0.0001 0.0007 0.0003
Bud Site 0.0049 0.0053 0.012 0.0003 0.0005 0.0067 0.0042 0.0023 0.0013 0.0011 0.0035 0.0002 0.0036 0.0012 0.0025 0.0003 0.0005 0.0002
Cell Periphery 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0856 0.1455 0.0929 0.0659 0.169 0.1363 0.2525 0.2102 0.1459 0.1202 0.1709 0.2564 0.1534 0.1656 0.1229 0.1639 0.1247 0.1935
Cytoplasmic Foci 0.0221 0.0078 0.0201 0.0143 0.0098 0.0108 0.0212 0.0085 0.0073 0.0178 0.0179 0.016 0.0267 0.0027 0.0093 0.0081 0.0042 0.0086
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0002 0.0002 0.0004 0.0003
Endoplasmic Reticulum 0.0071 0.004 0.003 0.0026 0.0044 0.0027 0.0097 0.0055 0.0032 0.0016 0.0039 0.0017 0.0072 0.0028 0.0027 0.0043 0.0017 0.002
Endosome 0.0136 0.0054 0.0104 0.0251 0.0064 0.0069 0.0171 0.0046 0.0086 0.0138 0.0279 0.0037 0.0171 0.0019 0.0049 0.0025 0.0025 0.0028
Golgi 0.0033 0.0001 0.0053 0.0011 0.0019 0.0031 0.0043 0.0001 0.0011 0.0136 0.014 0.0003 0.005 0.0001 0.0015 0.0001 0.0041 0.0009
Lipid Particles 0.011 0.0021 0.0087 0.0091 0.0017 0.0016 0.0095 0.0009 0.0051 0.0076 0.0275 0.0005 0.01 0.0002 0.0026 0.0003 0.0076 0.0006
Mitochondria 0.0112 0.0005 0.0071 0.002 0.0015 0.0066 0.0072 0.0005 0.0015 0.006 0.0185 0.0048 0.0024 0.0004 0.0012 0.0007 0.0023 0.0008
None 0.7187 0.6326 0.3841 0.707 0.5593 0.7557 0.5273 0.4871 0.242 0.2611 0.5081 0.6377 0.6055 0.6206 0.3807 0.4546 0.6383 0.6627
Nuclear Periphery 0.0229 0.0188 0.0249 0.0243 0.0196 0.0074 0.02 0.01 0.037 0.0145 0.0087 0.0029 0.0144 0.0071 0.0139 0.0113 0.005 0.0074
Nucleolus 0.0019 0.0029 0.0085 0.0051 0.0032 0.0013 0.0025 0.0028 0.007 0.0054 0.0017 0.0011 0.0017 0.0021 0.005 0.0017 0.0014 0.0015
Nucleus 0.0413 0.1453 0.3561 0.0895 0.1616 0.0339 0.0628 0.2338 0.5062 0.4683 0.1499 0.0582 0.0535 0.1859 0.4106 0.3211 0.15 0.1017
Peroxisomes 0.0083 0.0002 0.0072 0.0016 0.0005 0.0006 0.0052 0.0002 0.0022 0.0117 0.0046 0.0003 0.0117 0.0001 0.0032 0.0001 0.0048 0.0013
Punctate Nuclear 0.0154 0.0243 0.0441 0.0406 0.0308 0.011 0.0112 0.0288 0.0177 0.0394 0.03 0.0111 0.0202 0.0065 0.0205 0.0263 0.0111 0.0055
Vacuole 0.004 0.0028 0.0037 0.0031 0.0022 0.0014 0.0115 0.003 0.003 0.0015 0.0046 0.0017 0.0075 0.0019 0.0059 0.0018 0.0004 0.001
Vacuole Periphery 0.0019 0.0004 0.0011 0.0018 0.0009 0.0004 0.0023 0.0004 0.0011 0.0004 0.0013 0.0004 0.0016 0.0002 0.0007 0.0004 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.3283 13.0956 10.0645 15.9108 26.116 27.7136 12.4133 11.3305 13.4949 24.6165
Translational Efficiency 0.4886 0.2499 0.2762 0.4317 0.291 0.4522 0.3203 0.3724 0.3098 0.4069

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2390 818 1385 1142 1073 1683 1460 374 3463 2501 2845 1516

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.39 730.83 965.00 859.23 713.83 720.23 874.36 872.47 659.69 723.70 918.49 862.50
Standard Deviation 66.67 94.97 121.58 101.98 81.48 86.31 94.13 114.01 80.25 89.37 117.45 105.23
Intensity Change Log 2 — 0.201893 0.602887 0.435402 — 0.012877 0.292647 0.289525 — 0.104982 0.447078 0.360063

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000212 0.000584 0.000605 0.000055 0.000542 0.000193 0.000527 0.000042 0.000434 0.000383 0.000586
Bud Neck 0.006632 0.016053 0.007745 0.012474 0.004784 0.030636 0.005867 0.014342 0.006060 0.025866 0.006781 0.012935
Bud Site 0.002231 0.004757 0.007349 0.018156 0.001802 0.012942 0.004240 0.007519 0.002098 0.010265 0.005753 0.015532
Cell Periphery 0.000139 0.000148 0.000127 0.000237 0.000162 0.000231 0.000103 0.000123 0.000146 0.000204 0.000115 0.000209
Cytoplasm 0.476121* 0.331900* 0.060422 0.118557* 0.447208* 0.345222* 0.104867* 0.073268 0.467162* 0.340865* 0.083230 0.107384*
Cytoplasmic Foci 0.083925 0.170250 0.000799 0.005543 0.104329 0.207057* 0.001908 0.002655 0.090247 0.195018* 0.001369 0.004830
Eisosomes 0.000070 0.000104 0.000024 0.000044 0.000085 0.000158 0.000025 0.000032 0.000075 0.000140 0.000025 0.000041
Endoplasmic Reticulum 0.000402 0.001128 0.007742 0.008826 0.000698 0.000736 0.003709 0.005883 0.000494 0.000864 0.005672 0.008100
Endosome 0.001502 0.010495 0.000787 0.005095 0.004607 0.013048 0.000983 0.003586 0.002464 0.012213 0.000888 0.004723
Golgi 0.000597 0.002887 0.000049 0.000831 0.000876 0.005219 0.000037 0.005324 0.000683 0.004456 0.000043 0.001939
Lipid Particles 0.003364 0.006237 0.000568 0.001186 0.005733 0.011707 0.000613 0.000874 0.004098 0.009918 0.000591 0.001109
Mitochondria 0.002357 0.006930 0.001577 0.006055 0.002282 0.016806 0.000921 0.003434 0.002334 0.013576 0.001240 0.005408
Mitotic Spindle 0.001545 0.004607 0.004602 0.021937 0.002362 0.009945 0.002843 0.022232 0.001798 0.008199 0.003699 0.022009
None 0.013704 0.004465 0.002510 0.007025 0.007531 0.005610 0.005110 0.009037 0.011791 0.005235 0.003844 0.007521
Nuclear Periphery 0.000309 0.000844 0.002105 0.003329 0.000728 0.000566 0.001787 0.003934 0.000439 0.000657 0.001942 0.003478
Nuclear Periphery Foci 0.000175 0.000599 0.000532 0.002338 0.000800 0.000309 0.000260 0.001209 0.000369 0.000404 0.000393 0.002059
Nucleolus 0.001642 0.003304 0.000524 0.001506 0.001645 0.002532 0.000707 0.004278 0.001643 0.002785 0.000618 0.002190
Nucleus 0.395186* 0.390793* 0.887526* 0.739012* 0.393908* 0.279424* 0.849951* 0.810279* 0.394790* 0.315849* 0.868243* 0.756594*
Peroxisomes 0.001436 0.011224 0.000172 0.001262 0.002207 0.020774 0.000171 0.000501 0.001675 0.017650 0.000171 0.001074
Vacuole 0.008373 0.032189 0.014065 0.043804 0.017845 0.034936 0.015529 0.027447 0.011308 0.034037 0.014817 0.039769
Vacuole Periphery 0.000255 0.000873 0.000190 0.002180 0.000354 0.001602 0.000174 0.003516 0.000285 0.001364 0.000182 0.002510

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.54 -5.74 -15.74 -10.00 -0.42 -3.08 -8.64 -5.95 -2.16 -4.69 -3.76 -7.33 -15.17 -3.77 -4.39
Bud Neck -5.05 -1.49 -6.69 -0.17 -5.15 -11.31 -1.18 -3.81 5.19 -3.33 -12.11 -1.40 -7.84 5.21 -6.89
Bud Site -2.97 -3.31 -6.03 -4.43 -2.86 -6.77 -1.89 -3.34 3.22 -1.68 -7.24 -3.67 -6.68 -0.76 -3.94
Cell Periphery -0.83 0.33 -1.95 -1.23 -2.06 -4.14 6.76 3.47 6.47 -1.68 -5.05 3.83 -0.86 3.18 -3.30
Cytoplasm 13.98 69.41* 53.44 21.73 -7.21 9.38 37.32 33.05 27.62 2.45 18.37 75.83 62.20 36.90 -3.22
Cytoplasmic Foci -11.96 31.76 29.94 24.25 -4.00 -15.00 23.97 23.92 39.27 -0.16 -22.09 39.54 38.02 45.72 -3.19
Eisosomes -6.12 10.47 1.35 7.42 -10.09 -10.11 12.29 3.94 13.08 -6.31 -13.21 14.89 3.15 15.90 -11.76
Endoplasmic Reticulum -7.56 -22.72 -21.81 -20.09 -2.29 -0.42 -19.00 -13.12 -13.06 -6.60 -5.47 -29.02 -25.23 -24.20 -7.53
Endosome -7.85 3.45 -3.64 5.88 -4.64 -7.85 5.71 2.15 9.39 -2.34 -13.48 6.53 -1.49 11.34 -5.15
Golgi -2.88 5.45 1.46 3.15 -3.05 -5.55 2.99 -0.65 1.92 -1.21 -6.70 5.73 -0.18 5.45 -1.84
Lipid Particles -5.10 7.14 5.35 10.36 -4.92 -5.54 9.68 8.15 11.60 -2.51 -8.52 11.27 9.02 14.04 -5.74
Mitochondria -3.14 1.50 -1.08 2.13 -2.03 -7.62 1.38 -0.08 8.32 -2.72 -8.75 2.38 -1.04 7.54 -2.79
Mitotic Spindle -2.02 -2.83 -7.54 -6.39 -6.07 -3.62 -0.97 -3.00 -1.60 -2.75 -4.68 -3.00 -8.07 -5.69 -6.96
None 10.74 14.38 8.54 -1.81 -5.58 1.30 2.33 -0.36 -1.69 -2.67 8.50 11.77 6.35 -1.55 -4.88
Nuclear Periphery -9.25 -20.53 -18.44 -16.03 -9.06 2.83 -16.57 -6.01 -6.32 -2.96 -7.00 -28.30 -18.62 -17.61 -9.84
Nuclear Periphery Foci -3.12 -4.27 -5.24 -3.74 -3.58 1.68 1.55 -2.21 -4.60 -4.47 -0.64 -1.71 -5.48 -5.40 -4.96
Nucleolus -4.07 4.95 -2.76 0.86 -5.38 -1.28 2.64 -2.18 -1.82 -3.21 -4.19 5.29 -3.44 -1.25 -6.02
Nucleus 0.50 -66.60 -31.86 -24.33 15.81 10.37 -44.71 -23.09 -31.95 4.85 10.67 -80.01 -38.90 -44.85 15.49
Peroxisomes -7.56 5.27 3.06 8.19 -2.28 -11.15 5.45 4.87 12.75 -2.19 -13.64 7.70 5.11 14.76 -2.88
Vacuole -10.98 -8.24 -15.46 -8.38 -12.07 -8.08 -2.75 -6.29 -4.11 -5.51 -16.52 -9.20 -16.36 -9.80 -13.10
Vacuole Periphery -6.48 1.62 -2.15 -0.12 -2.37 -6.55 4.41 -1.75 0.39 -2.08 -8.23 3.76 -2.73 1.13 -3.14
Standard name
Human Ortholog
Description 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (28%), nucleus (5%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Exo1

Exo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available