Standard name
Human Ortholog
Description Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.98 0.97 1.0 0.97 0.99 0.97 0.95 0.93 0.83 0.81 0.78 0.82 1.0 0.99 1.0 0.97 0.93 0.9 0.94 0.9 0.84 0.78 0.74 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.07 0.1 0.05 0.31 0.28 0.38 0.23 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 0 2 3 0 0 0 0 0 0 0 1 0 0 1 2 2 2 5 5
Bud 1 0 1 2 1 3 6 2 8 13 10 12 1 0 2 0 0 0 0 2 1 3 8 4
Bud Neck 0 1 10 2 2 1 4 7 8 4 0 3 0 0 0 0 0 0 0 2 3 4 5 6
Bud Site 0 0 0 0 0 0 0 0 2 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 5 2 7 2 2 6 7 15 4 2 3 4 3 8 13 11 0 0 4 1 3 2
Cytoplasm 136 239 288 181 349 428 598 596 447 450 300 339 241 335 463 176 126 90 135 223 246 173 265 323
Endoplasmic Reticulum 0 5 0 0 1 1 0 3 1 0 2 1 1 1 0 6 8 1 1 0 1 6 13 5
Endosome 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 1 1 0 3 1 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 1 0 1 0 1
Mitochondria 2 9 0 10 2 33 61 35 167 155 147 95 0 4 2 1 0 1 1 6 20 17 36 19
Nucleus 0 0 0 4 2 0 2 10 1 1 2 1 0 0 2 1 1 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 1 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 5 0 2 1 5 0 0 0 1 0 1 0 0 1 0 0 1
Vac/Vac Membrane 0 0 0 0 1 1 2 7 1 9 5 8 0 0 1 4 0 2 1 2 1 8 14 12
Unique Cell Count 139 247 289 187 352 442 630 639 540 553 386 412 242 337 465 182 135 100 144 249 292 221 360 392
Labelled Cell Count 141 258 306 203 365 470 678 675 643 652 475 467 246 344 473 200 151 111 144 249 292 221 360 392


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 5.1 4.8 5.2 4.3 4.6 4.9 4.0 4.0 3.8 4.3 4.8 5.1 4.9 6.9 7.1 6.8 4.6 4.5 4.8
Std Deviation (1e-4) 0.3 0.8 0.9 1.4 1.1 0.8 1.1 1.0 1.0 0.8 1.2 0.7 0.8 1.3 1.1 1.2 2.1 1.1 1.3 1.3 1.0
Intensity Change (Log2) – – – -0.09 0.04 -0.26 -0.15 -0.06 -0.35 -0.35 -0.41 -0.24 -0.09 -0.01 -0.05 0.44 0.48 0.41 -0.14 -0.17 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3685 3.1209 2.8869 2.6619 2.6198 2.8513 0.3025 0.7053 0.4227 0.3122 0.1248 0.3657 -0.1339 1.0247 0.5342 0.5573 0.1308 0.1485
Actin 0.0228 0.0008 0.0083 0.0001 0.0379 0.0031 0.04 0.002 0.014 0.0024 0.0141 0.0069 0.0258 0.001 0.0294 0.0686 0.0355 0.0125
Bud 0.0008 0.0006 0.0008 0.0002 0.0025 0.0003 0.0013 0.0026 0.003 0.0002 0.0049 0.0021 0.0003 0.001 0.0022 0.0002 0.0002 0.0002
Bud Neck 0.0056 0.0011 0.0008 0.0002 0.0098 0.0065 0.0041 0.0016 0.0012 0.0009 0.0056 0.0025 0.0113 0.0002 0.001 0.0002 0.0004 0.0006
Bud Periphery 0.0016 0.0006 0.0011 0.0002 0.0052 0.0012 0.002 0.0032 0.0038 0.0003 0.0121 0.0018 0.0004 0.0006 0.002 0.0001 0.0002 0.0002
Bud Site 0.0074 0.0114 0.0033 0.0003 0.0028 0.0001 0.0062 0.0108 0.0068 0.0002 0.002 0.0004 0.0008 0.0111 0.0044 0.0002 0.0002 0.0001
Cell Periphery 0.0015 0.001 0.001 0.0001 0.0006 0.0003 0.0005 0.001 0.0004 0.0002 0.0004 0.0002 0.0005 0.0006 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.418 0.6113 0.5542 0.6719 0.4782 0.6234 0.5962 0.7572 0.7138 0.75 0.5715 0.8315 0.6416 0.7715 0.6737 0.7548 0.7497 0.8077
Cytoplasmic Foci 0.0253 0.0092 0.0188 0.0069 0.011 0.0132 0.0453 0.0213 0.0307 0.0285 0.058 0.0169 0.026 0.0126 0.0264 0.0188 0.0131 0.0162
Eisosomes 0.0013 0.0001 0.0003 0 0.0005 0.0001 0.0003 0 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0083 0.0025 0.0034 0.0011 0.003 0.0029 0.0074 0.0041 0.0045 0.0036 0.0031 0.0036 0.0107 0.004 0.0064 0.0162 0.0057 0.0037
Endosome 0.0154 0.0049 0.0061 0.0042 0.0139 0.0113 0.0448 0.0178 0.026 0.0277 0.0444 0.0135 0.0255 0.0059 0.0213 0.0252 0.0158 0.0148
Golgi 0.0045 0.0015 0.0023 0.0003 0.0079 0.0025 0.0102 0.0014 0.0084 0.0017 0.0161 0.0013 0.0065 0.0005 0.0048 0.0015 0.0073 0.0021
Lipid Particles 0.0078 0.0002 0.0019 0.0001 0.0012 0.0014 0.0096 0.001 0.0036 0.0002 0.0037 0.0018 0.0159 0.0008 0.0027 0.0036 0.0492 0.0004
Mitochondria 0.0024 0.0007 0.0102 0.0002 0.0252 0.0009 0.007 0.0006 0.0082 0.0005 0.0558 0.0007 0.0034 0.0001 0.0024 0.0003 0.0015 0.0015
None 0.4408 0.3504 0.3702 0.3118 0.3923 0.3221 0.1946 0.1394 0.1589 0.1752 0.1789 0.107 0.211 0.1846 0.2078 0.1029 0.0921 0.1355
Nuclear Periphery 0.0038 0.0003 0.0006 0.0003 0.001 0.0052 0.0057 0.0011 0.0018 0.0008 0.0017 0.0015 0.0042 0.0008 0.0017 0.0012 0.0066 0.0005
Nucleolus 0.0012 0.0001 0.0003 0.0001 0.0002 0.0001 0.0008 0.0068 0.0002 0.0001 0.0007 0.0005 0.0002 0.0001 0.0003 0 0.0001 0
Nucleus 0.0069 0.0007 0.0012 0.0005 0.0008 0.0019 0.0023 0.0036 0.0017 0.0013 0.0015 0.002 0.0009 0.0009 0.0012 0.0006 0.0011 0.0007
Peroxisomes 0.0097 0.0001 0.0117 0.0001 0.0018 0.0009 0.01 0.0003 0.0052 0.0007 0.0072 0.0011 0.0082 0.0001 0.0048 0.0012 0.016 0.0011
Punctate Nuclear 0.0113 0.0003 0.0008 0.0002 0.0012 0.0011 0.0046 0.001 0.0024 0.0007 0.002 0.0025 0.0013 0.0008 0.003 0.0004 0.0029 0.0003
Vacuole 0.0029 0.002 0.0023 0.001 0.0022 0.001 0.0056 0.0199 0.0046 0.0038 0.0102 0.0019 0.0036 0.0025 0.0035 0.0031 0.0016 0.0014
Vacuole Periphery 0.0005 0.0002 0.0003 0.0002 0.0007 0.0004 0.0014 0.0033 0.0008 0.0007 0.0058 0.0003 0.0017 0.0002 0.0006 0.0006 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0786 8.74 10.5003 16.8983 10.2618 13.4941 17.9897 17.0412 17.1977 17.6482
Translational Efficiency 0.7245 0.9392 0.8055 0.6685 0.7676 0.7536 0.689 0.58 0.7452 0.6826

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1208 1261 192 1209 1765 1328 2007 1244 2973 2589 2199 2453

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.24 794.34 923.55 877.52 688.90 764.08 866.19 865.86 666.69 778.82 871.20 871.61
Standard Deviation 77.13 99.27 97.13 117.16 95.33 95.78 96.41 105.48 92.37 98.66 97.82 111.54
Intensity Change Log 2 — 0.324728 0.542161 0.468403 — 0.149429 0.330389 0.329839 — 0.236118 0.435784 0.397921

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000315 0.000207 0.001351 0.005838 0.000162 0.000456 0.000135 0.002195 0.000224 0.000335 0.000241 0.003991
Bud Neck 0.030836 0.017062 0.002329 0.008053 0.016178 0.047498 0.002914 0.003816 0.022134 0.032674 0.002863 0.005904
Bud Site 0.010123 0.012475 0.017036 0.047491 0.005791 0.027271 0.010818 0.035854 0.007551 0.020064 0.011361 0.041589
Cell Periphery 0.000258 0.000118 0.000327 0.000382 0.000135 0.000266 0.000127 0.000368 0.000185 0.000193 0.000144 0.000375
Cytoplasm 0.482510* 0.564689* 0.439134* 0.492673* 0.529808* 0.384761* 0.663443* 0.545581* 0.510590* 0.472397* 0.643858* 0.519505*
Cytoplasmic Foci 0.246360* 0.225496* 0.040892 0.049387 0.243006* 0.307863* 0.024487 0.048793 0.244369* 0.267745* 0.025919 0.049086
Eisosomes 0.000361 0.000116 0.000112 0.000145 0.000184 0.000393 0.000036 0.000140 0.000256 0.000258 0.000042 0.000142
Endoplasmic Reticulum 0.000943 0.000752 0.006364 0.001861 0.001429 0.001046 0.000894 0.001655 0.001231 0.000903 0.001372 0.001756
Endosome 0.030911 0.021775 0.009162 0.007016 0.018811 0.052951 0.001454 0.004792 0.023728 0.037766 0.002127 0.005888
Golgi 0.003868 0.004754 0.000172 0.003057 0.004754 0.014314 0.000075 0.002347 0.004394 0.009658 0.000083 0.002697
Lipid Particles 0.010705 0.003684 0.003802 0.001238 0.007875 0.006076 0.000683 0.001306 0.009025 0.004911 0.000956 0.001272
Mitochondria 0.006300 0.002502 0.000238 0.006872 0.004359 0.008531 0.000279 0.000794 0.005148 0.005594 0.000275 0.003790
Mitotic Spindle 0.000415 0.001741 0.001872 0.018780 0.000635 0.003301 0.002159 0.007737 0.000546 0.002541 0.002134 0.013180
None 0.003243 0.001790 0.002581 0.000950 0.001916 0.000864 0.002414 0.000978 0.002455 0.001315 0.002428 0.000964
Nuclear Periphery 0.000333 0.000183 0.001396 0.000359 0.000335 0.000109 0.000588 0.000350 0.000334 0.000145 0.000658 0.000354
Nuclear Periphery Foci 0.000300 0.000663 0.005686 0.001737 0.000536 0.000185 0.000881 0.001778 0.000440 0.000418 0.001300 0.001758
Nucleolus 0.001546 0.000469 0.000349 0.000158 0.000875 0.000520 0.000135 0.000088 0.001147 0.000495 0.000154 0.000122
Nucleus 0.060747 0.052330 0.225554* 0.070214 0.077292 0.025161 0.208985* 0.092490 0.070569 0.038394 0.210432* 0.081511
Peroxisomes 0.006724 0.004142 0.001036 0.002026 0.003615 0.014559 0.000190 0.000937 0.004878 0.009485 0.000264 0.001474
Vacuole 0.101012 0.083354 0.240362* 0.279787* 0.080819 0.100419 0.079146 0.247263* 0.089024 0.092108 0.093222 0.263293*
Vacuole Periphery 0.002189 0.001698 0.000244 0.001976 0.001486 0.003456 0.000159 0.000739 0.001772 0.002600 0.000167 0.001348

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.05 -2.50 -2.56 -2.68 -1.73 -5.66 0.34 -2.21 -1.68 -2.22 -2.27 -0.26 -3.35 -3.16 -3.33
Bud Neck 5.56 12.71 11.54 7.32 -1.86 -11.57 10.27 9.79 18.06 -1.04 -5.79 16.50 14.72 18.81 -2.72
Bud Site -1.63 -0.36 -6.47 -5.62 -3.27 -11.01 -2.20 -7.22 0.88 -5.37 -10.40 -1.16 -9.94 -2.94 -7.65
Cell Periphery 8.16 -2.12 -0.82 -9.13 1.50 -6.52 1.25 -8.72 -1.32 -9.09 -0.68 4.28 -7.22 -5.93 -10.47
Cytoplasm -7.63 7.05 6.38 13.39 -3.58 15.21 -8.40 6.69 -6.88 13.94 5.27 -8.98 9.77 4.45 17.09
Cytoplasmic Foci 2.72 33.54 36.20 31.96 -2.35 -8.53 44.87 40.05 43.29 -7.78 -4.23 58.91 53.96 52.63 -9.07
Eisosomes 7.81 7.03 7.45 -1.26 0.64 -6.65 15.90 4.61 8.40 -12.57 -0.10 14.95 8.74 7.60 -15.47
Endoplasmic Reticulum 0.80 -9.94 -3.28 -4.78 8.63 0.92 0.63 -1.82 -2.38 -5.29 1.33 -3.57 -3.33 -4.55 0.01
Endosome 4.03 11.47 14.35 10.83 3.35 -13.78 16.73 13.30 21.48 -2.51 -8.37 22.94 19.27 23.32 -2.42
Golgi -1.18 9.42 4.18 4.55 -2.19 -8.73 14.06 6.11 11.44 -2.15 -7.90 16.96 7.46 12.00 -3.18
Lipid Particles 7.29 6.87 10.42 6.02 7.80 2.64 12.12 10.99 12.36 -8.24 7.32 15.72 15.13 13.04 -4.50
Mitochondria 2.88 5.18 2.20 -0.25 -2.27 -2.85 4.33 4.08 7.08 -1.01 -0.44 6.65 3.87 4.62 -2.41
Mitotic Spindle -2.28 -2.64 -5.26 -4.54 -4.68 -2.43 -2.70 -3.03 -0.84 -1.64 -3.15 -3.12 -6.03 -4.09 -4.37
None 2.17 1.92 3.48 2.14 2.57 3.31 -1.45 4.22 -0.34 8.32 3.37 0.75 4.97 1.48 8.55
Nuclear Periphery 2.09 -9.85 -3.67 -9.47 8.85 4.97 -9.61 -3.66 -13.23 7.75 4.72 -13.01 -5.31 -15.62 10.75
Nuclear Periphery Foci -2.20 -9.37 -13.33 -5.16 7.16 5.66 -5.30 -9.53 -13.64 -5.23 0.28 -10.51 -15.03 -11.75 -1.72
Nucleolus 2.57 2.90 3.28 6.38 2.96 3.39 8.01 9.13 6.72 2.98 3.58 5.26 5.60 9.17 2.15
Nucleus 2.57 -11.11 -4.82 -7.20 10.03 19.50 -30.06 -5.33 -20.66 23.26 15.30 -34.46 -6.60 -19.63 28.96
Peroxisomes 2.81 6.76 6.19 6.31 -0.77 -8.48 12.04 9.64 11.06 -2.96 -5.82 11.85 9.51 12.43 -3.04
Vacuole 3.40 -11.51 -32.14 -34.58 -4.05 -4.50 -8.52 -31.68 -28.32 -25.44 -1.16 -10.59 -45.46 -44.32 -32.89
Vacuole Periphery 1.48 8.80 3.60 2.20 -1.87 -5.61 9.67 4.48 7.95 -1.10 -3.45 12.88 5.32 7.19 -2.31
Standard name
Human Ortholog
Description Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

She4

She4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (She4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available