Standard name
Human Ortholog
Description Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0.05 0.07 0 0 – 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.15 0.43 0.31 0.34 0.21 0.23 0.22 0.24 – 0.25 0.19 0.22 0.69 0.67 0.78 0.5 0.55 0.48 0 0.07 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.05 0.06 0.07 0.11 0.07 0 0 0 0 0 0
Endosome 0.18 0.1 0.05 0.09 0.07 0 0 0 – 0 0 0 0 0.13 0 0.08 0.1 0.14 0.11 0.08 0.1 0.06 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.35 0.55 0.57 0.73 0.82 0.82 0.76 – 0.76 0.86 0.83 0 0 0 0.19 0.09 0.08 0.67 0.61 0.68 0.63 0.64 0.63
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.07 0.07 0.05 0.05 0.06 – 0.08 0.05 0.06 0 0 0 0.17 0.1 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.12 0 0 0.08 0 0 0 – 0 0 0 0.06 0.08 0.05 0.05 0.09 0.13 0.05 0.07 0 0.1 0.1 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 1 0 0 0 0 0 – 0 0 0 16 1 3 5 2 2 15 28 22 12 19 13
Bud 8 10 32 11 29 68 43 31 – 54 60 57 5 1 3 6 5 6 7 7 6 4 13 9
Bud Neck 0 1 5 0 0 2 1 2 – 0 2 1 6 0 4 1 12 1 5 10 5 8 18 17
Bud Site 0 0 0 1 5 14 8 7 – 11 13 10 0 0 0 1 0 0 – – – – – –
Cell Periphery 13 14 10 5 4 0 5 4 – 4 6 12 9 3 5 18 14 15 0 6 2 0 2 4
Cytoplasm 78 193 158 137 129 223 233 226 – 245 163 244 173 115 168 164 150 127 9 44 11 11 13 14
Endoplasmic Reticulum 5 8 9 0 0 1 2 1 – 1 1 1 17 8 12 24 29 19 5 11 7 3 5 4
Endosome 96 46 24 37 40 13 12 13 – 16 0 9 6 23 9 26 27 38 78 49 62 20 31 33
Golgi 3 0 1 0 0 0 0 0 – 0 0 0 1 0 0 5 5 3 20 11 11 2 7 8
Mitochondria 318 155 280 231 440 809 883 702 – 754 725 897 5 6 4 61 25 21 470 393 432 221 377 328
Nucleus 6 0 3 3 3 21 16 20 – 6 4 11 3 1 2 5 4 2 0 3 1 1 2 2
Nuclear Periphery 4 0 0 0 1 0 1 1 – 2 1 0 0 0 0 0 0 0 9 1 4 0 3 1
Nucleolus 2 0 0 0 2 5 9 9 – 2 3 4 0 0 0 0 0 0 4 0 1 0 3 1
Peroxisomes 18 7 27 33 27 12 15 16 – 18 1 16 2 3 3 14 3 32 12 5 18 9 5 9
SpindlePole 21 9 38 29 45 54 50 60 – 83 38 64 11 2 3 55 28 28 13 13 16 9 15 10
Vac/Vac Membrane 88 54 17 16 47 27 32 29 – 40 23 14 15 13 10 15 26 33 37 47 19 35 59 54
Unique Cell Count 528 447 508 405 605 987 1079 928 996 840 1086 251 171 215 326 275 263 705 645 637 352 591 521
Labelled Cell Count 660 503 605 503 772 1249 1310 1121 1236 1040 1340 269 176 226 400 330 327 705 645 637 352 591 521


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.5 4.0 4.1 3.7 3.6 3.1 3.3 – 3.0 2.8 2.9 5.5 5.3 5.3 5.7 5.5 5.9 6.1 5.9 5.8
Std Deviation (1e-4) 0.9 1.0 1.1 1.7 1.6 1.3 1.3 1.2 – 1.2 0.6 0.7 0.8 1.0 0.9 1.2 1.3 5.5 1.7 1.4 1.3
Intensity Change (Log2) – – – 0.03 -0.11 -0.16 -0.39 -0.28 – -0.39 -0.51 -0.47 0.45 0.4 0.41 0.5 0.47 0.55 0.6 0.55 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.9 3.6 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1689 0.6246 0.3193 0.3864 0.0453 0.4296 2.7163 3.2419 3.0797 2.7678 3.1165 3.4758 1.8032 1.8417 1.6535 1.7374 1.7582 1.8534
Actin 0.0083 0.0296 0.006 0.0105 0.0095 0.0118 0.0191 0.0216 0.0278 0.0138 0.0709 0.0157 0.0155 0.0099 0.0048 0.004 0.0043 0.0068
Bud 0.0028 0.0035 0.0159 0.0084 0.0121 0.0071 0.0018 0.0064 0.0054 0.0061 0.0105 0.002 0.0348 0.0548 0.0684 0.0424 0.0448 0.0324
Bud Neck 0.0023 0.0025 0.0091 0.0279 0.0011 0.007 0.0021 0.0029 0.0031 0.0434 0.0023 0.005 0.0037 0.0027 0.0067 0.0032 0.0031 0.008
Bud Periphery 0.005 0.0048 0.0087 0.0206 0.0105 0.005 0.004 0.0179 0.0162 0.0192 0.0462 0.0061 0.0197 0.0192 0.0235 0.0179 0.0281 0.021
Bud Site 0.0134 0.027 0.0238 0.0053 0.0159 0.0032 0.0077 0.0317 0.0157 0.0074 0.0077 0.0025 0.0349 0.1081 0.0723 0.0128 0.0177 0.0081
Cell Periphery 0.0022 0.0037 0.0028 0.0017 0.0011 0.0018 0.0015 0.002 0.0018 0.0024 0.0086 0.0011 0.0036 0.0032 0.0029 0.0008 0.0039 0.0018
Cytoplasm 0.0265 0.0592 0.034 0.0833 0.0251 0.0512 0.0349 0.0757 0.0502 0.0832 0.0631 0.0485 0.0698 0.0851 0.0956 0.0946 0.1252 0.1603
Cytoplasmic Foci 0.1181 0.1373 0.1018 0.1678 0.1119 0.1252 0.1399 0.1246 0.1136 0.1123 0.1583 0.1115 0.1468 0.1198 0.1223 0.1132 0.1743 0.1379
Eisosomes 0.0016 0.0075 0.0011 0.0013 0.0016 0.0007 0.0007 0.001 0.001 0.0005 0.0005 0.0006 0.0008 0.0005 0.0007 0.0002 0.001 0.0003
Endoplasmic Reticulum 0.0073 0.0032 0.0029 0.0043 0.0012 0.0033 0.0034 0.0024 0.0025 0.0017 0.0013 0.0018 0.0078 0.0049 0.0038 0.0025 0.007 0.0038
Endosome 0.1315 0.1777 0.096 0.0639 0.091 0.0944 0.1904 0.1127 0.1465 0.1195 0.1462 0.1214 0.1552 0.1474 0.0985 0.15 0.0813 0.1012
Golgi 0.0419 0.0561 0.0308 0.0171 0.0382 0.0347 0.0742 0.0703 0.0548 0.0618 0.0663 0.0657 0.0359 0.0381 0.0336 0.0267 0.0273 0.0302
Lipid Particles 0.0531 0.022 0.0203 0.0168 0.0085 0.0222 0.035 0.0115 0.0121 0.0081 0.0032 0.0203 0.0226 0.0085 0.0096 0.0205 0.0173 0.0101
Mitochondria 0.2992 0.2855 0.3396 0.3041 0.4935 0.3435 0.3013 0.3744 0.3408 0.3166 0.3244 0.3816 0.1722 0.1705 0.1896 0.2683 0.2012 0.1684
None 0.2111 0.0796 0.204 0.1703 0.1003 0.1522 0.1013 0.0623 0.1104 0.1344 0.022 0.129 0.181 0.1357 0.1665 0.1271 0.1341 0.1874
Nuclear Periphery 0.0028 0.0014 0.004 0.0012 0.0009 0.0021 0.0036 0.0007 0.0019 0.0011 0.0005 0.0007 0.0065 0.0026 0.0033 0.004 0.0048 0.0036
Nucleolus 0.0062 0.0073 0.0051 0.0018 0.0014 0.0046 0.0017 0.0014 0.0017 0.0019 0.0005 0.0006 0.0104 0.0079 0.0075 0.0056 0.0067 0.0066
Nucleus 0.0035 0.0026 0.0045 0.0038 0.004 0.0068 0.0028 0.002 0.0029 0.0025 0.0011 0.0008 0.0114 0.0158 0.0202 0.0182 0.0151 0.0288
Peroxisomes 0.0369 0.0675 0.0528 0.0407 0.0606 0.0944 0.0483 0.065 0.0642 0.0383 0.0532 0.074 0.0197 0.0205 0.0255 0.0371 0.0587 0.0375
Punctate Nuclear 0.0072 0.0051 0.0137 0.0058 0.0038 0.0154 0.0041 0.0009 0.0079 0.0027 0.0008 0.0015 0.0194 0.0153 0.023 0.0235 0.0175 0.0299
Vacuole 0.0146 0.0127 0.0166 0.02 0.0053 0.0092 0.0165 0.0094 0.0133 0.0181 0.0085 0.0065 0.022 0.0226 0.0159 0.0202 0.0201 0.0109
Vacuole Periphery 0.0045 0.0041 0.0066 0.0234 0.0025 0.0042 0.0056 0.0032 0.006 0.0048 0.0039 0.0033 0.0063 0.007 0.0059 0.0072 0.0065 0.0048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5164 8.9589 4.6535 10.2643 7.1466 5.0081 8.379 11.6162 8.6166 11.7218
Translational Efficiency 1.1025 0.7451 1.4059 0.5064 0.848 2.0692 1.0718 0.7692 1.0147 1.0173

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
439 1167 171 1423 1951 1246 1399 1434 2390 2413 1570 2857

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 619.58 744.46 829.88 806.40 678.02 706.33 794.96 809.19 667.29 724.77 798.76 807.80
Standard Deviation 75.05 108.28 94.35 120.91 75.81 92.10 98.68 130.60 78.98 102.05 98.82 125.88
Intensity Change Log 2 — 0.264904 0.421612 0.380205 — 0.059015 0.229554 0.255151 — 0.160993 0.324452 0.316215

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000329 0.002214 0.007015 0.004025 0.000472 0.003351 0.005168 0.002904 0.000446 0.002801 0.005369 0.003462
Bud Neck 0.047117 0.033974 0.017651 0.033067 0.041422 0.026941 0.063950 0.040828 0.042468 0.030343 0.058907 0.036963
Bud Site 0.012449 0.035967 0.035337 0.078210 0.015479 0.030875 0.080865 0.075066 0.014922 0.033338 0.075907 0.076632
Cell Periphery 0.000275 0.000285 0.000327 0.000299 0.000161 0.000366 0.000206 0.000224 0.000182 0.000327 0.000219 0.000261
Cytoplasm 0.193326* 0.042616 0.176437* 0.343373* 0.061144 0.032932 0.256587* 0.329089* 0.085423 0.037615 0.247857* 0.336203*
Cytoplasmic Foci 0.382992* 0.196119* 0.173946 0.089541 0.256741* 0.160833 0.104178 0.131650 0.279931* 0.177898 0.111777 0.110677
Eisosomes 0.000296 0.000191 0.000224 0.000132 0.000177 0.000282 0.000134 0.000133 0.000199 0.000238 0.000144 0.000133
Endoplasmic Reticulum 0.001222 0.001918 0.015224 0.003524 0.001077 0.000806 0.004833 0.002153 0.001104 0.001344 0.005965 0.002836
Endosome 0.050422 0.080252 0.050681 0.016748 0.091114 0.094806 0.020736 0.024296 0.083639 0.087767 0.023998 0.020536
Golgi 0.016914 0.088980 0.007966 0.008342 0.063138 0.120940* 0.009170 0.010209 0.054647 0.105483 0.009039 0.009279
Lipid Particles 0.027151 0.010476 0.018861 0.003410 0.016613 0.007444 0.004585 0.003862 0.018548 0.008910 0.006140 0.003637
Mitochondria 0.080798 0.298067* 0.004412 0.010759 0.311918* 0.367593* 0.040227 0.021584 0.269465* 0.333968* 0.036326 0.016193
Mitotic Spindle 0.002497 0.028507 0.037947 0.066310 0.010912 0.013903 0.060885 0.042909 0.009366 0.020966 0.058386 0.054565
None 0.003229 0.001472 0.004919 0.003867 0.000727 0.000429 0.001694 0.005732 0.001186 0.000933 0.002045 0.004803
Nuclear Periphery 0.000939 0.000653 0.002943 0.001612 0.000630 0.000270 0.001466 0.000890 0.000687 0.000455 0.001627 0.001249
Nuclear Periphery Foci 0.000581 0.000705 0.014034 0.002033 0.000282 0.000266 0.000940 0.001044 0.000337 0.000478 0.002366 0.001537
Nucleolus 0.004085 0.003932 0.005048 0.001887 0.002495 0.000980 0.001909 0.001702 0.002787 0.002408 0.002251 0.001794
Nucleus 0.089220 0.024641 0.205450* 0.143969* 0.014711 0.005197 0.157450* 0.108734 0.028397 0.014601 0.162678* 0.126283
Peroxisomes 0.045000 0.077311 0.058979 0.025926 0.045395 0.093271 0.060340 0.039549 0.045322 0.085552 0.060191 0.032764
Vacuole 0.034969 0.056667 0.158656 0.157541 0.049544 0.027524 0.121590 0.152912 0.046866 0.041619 0.125628 0.155218
Vacuole Periphery 0.006190 0.015053 0.003943 0.005426 0.015851 0.010991 0.003086 0.004531 0.014076 0.012955 0.003180 0.004977

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.42 -3.59 -7.60 -1.39 2.01 -5.05 -10.52 -5.26 1.48 4.49 -6.52 -11.04 -8.81 0.31 4.84
Bud Neck 2.08 5.03 3.43 2.28 -3.42 4.53 -4.80 1.60 -3.12 6.48 4.35 -3.65 4.07 -0.63 7.88
Bud Site -7.14 -4.75 -10.60 -4.66 -4.06 -6.74 -14.82 -11.75 -6.86 1.74 -10.13 -15.39 -16.15 -8.18 1.76
Cell Periphery -0.25 -1.47 0.93 1.48 3.29 -7.10 -2.46 -1.94 5.95 0.36 -6.53 -2.11 -2.04 5.10 -0.06
Cytoplasm 14.42 1.32 -10.63 -36.49 -9.59 7.86 -27.01 -33.62 -37.63 -6.91 13.49 -23.28 -39.87 -52.45 -9.77
Cytoplasmic Foci 14.97 13.28 25.97 19.90 7.09 14.42 27.80 22.47 7.23 -3.68 17.97 31.67 35.46 18.81 1.98
Eisosomes 3.77 2.09 6.96 5.04 6.69 -5.11 4.74 5.99 8.05 1.45 -2.78 5.90 9.02 9.46 3.72
Endoplasmic Reticulum -2.01 -7.87 -6.95 -5.89 6.60 1.36 -11.50 -5.10 -6.24 7.92 -1.48 -13.63 -10.15 -8.61 8.96
Endosome -4.95 0.46 7.74 18.30 5.73 -0.73 22.60 21.67 18.06 -1.14 -1.04 21.44 24.56 25.60 2.97
Golgi -15.04 3.95 5.46 18.71 0.80 -11.03 22.51 21.79 23.59 -0.21 -13.36 22.38 23.08 29.95 0.81
Lipid Particles 5.24 2.25 8.07 6.49 6.82 6.33 9.12 10.24 4.56 2.20 7.21 9.94 13.02 7.93 5.81
Mitochondria -17.92 9.04 8.65 32.92* -2.73 -4.91 38.39* 41.91* 37.71* 5.22 -7.22 37.07 41.83* 49.77* 7.09
Mitotic Spindle -8.96 -4.68 -11.59 -4.71 -1.64 -1.58 -11.00 -7.68 -6.74 3.54 -6.45 -12.01 -12.57 -7.86 2.02
None 1.87 -0.49 0.21 -1.85 0.59 2.98 -6.67 -3.42 -3.69 -2.54 0.83 -2.44 -3.86 -4.10 -2.52
Nuclear Periphery 0.61 -2.99 -3.02 -6.69 1.44 1.72 -5.04 -3.52 -5.14 2.25 1.24 -5.61 -6.37 -8.44 1.13
Nuclear Periphery Foci -0.79 -3.87 -3.71 -3.14 3.50 0.20 -8.28 -4.37 -4.26 -0.39 -1.65 -5.42 -6.31 -5.00 2.29
Nucleolus 0.31 -0.70 3.43 3.40 3.45 2.79 1.13 1.47 -2.31 0.83 0.84 1.22 2.45 1.81 2.52
Nucleus 8.17 -6.03 -9.24 -24.06 1.74 6.07 -27.00 -23.55 -26.03 7.07 5.84 -25.86 -30.12 -34.79 4.73
Peroxisomes -5.38 -0.82 5.75 12.86 3.92 -9.46 -1.43 4.14 11.88 4.74 -10.90 -1.62 8.09 17.40 7.66
Vacuole -5.30 -9.93 -26.75 -23.38 -2.45 7.86 -19.46 -24.11 -27.91 -6.46 1.65 -21.98 -35.22 -36.11 -8.89
Vacuole Periphery -6.63 2.56 2.56 9.32 0.07 4.28 18.00 13.07 7.06 -1.28 1.27 17.98 14.01 11.61 -1.50
Standard name
Human Ortholog
Description Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
Localization
Cell Percentages mitochondrion (69%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cyc2

Cyc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available