Standard name | |
---|---|
Human Ortholog | |
Description | Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 19 | 4 | 4 | 1 | 5 | 6 | 4 | 9 | 2 | 4 | 4 | 6 | 31 | 39 | 35 | 20 | 27 | 12 | 11 | 6 | 1 | 9 | 9 | 13 |
Bud | 10 | 2 | 5 | 2 | 3 | 2 | 6 | 3 | 5 | 1 | 1 | 1 | 7 | 8 | 3 | 2 | 4 | 8 | 2 | 0 | 0 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 0 | 6 | 7 | 6 | 8 | 11 | 26 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 106 | 47 | 32 | 14 | 19 | 47 | 47 | 45 | 44 | 37 | 24 | 55 | 264 | 309 | 366 | 104 | 85 | 81 | 172 | 53 | 51 | 223 | 282 | 356 |
Cytoplasm | 172 | 73 | 83 | 20 | 45 | 71 | 101 | 138 | 86 | 77 | 54 | 71 | 302 | 391 | 511 | 250 | 137 | 106 | 35 | 26 | 21 | 33 | 28 | 44 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 1 | 2 | 4 | 2 | 4 | 18 | 19 | 6 | 10 | 1 | 3 | 3 | 13 | 13 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 1 | 0 | 2 | 3 | 0 |
Mitochondria | 17 | 3 | 2 | 0 | 3 | 11 | 29 | 18 | 44 | 28 | 34 | 47 | 6 | 3 | 2 | 17 | 13 | 12 | 17 | 3 | 3 | 5 | 5 | 7 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 5 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 2 | 2 | 1 | 1 | 0 | 4 |
Unique Cell Count | 223 | 96 | 91 | 22 | 55 | 91 | 135 | 170 | 131 | 108 | 85 | 125 | 412 | 526 | 623 | 327 | 220 | 179 | 269 | 104 | 94 | 295 | 364 | 481 |
Labelled Cell Count | 330 | 130 | 128 | 37 | 75 | 138 | 191 | 216 | 184 | 149 | 120 | 185 | 616 | 753 | 927 | 427 | 299 | 228 | 269 | 104 | 94 | 295 | 364 | 481 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.2 | 5.1 | 5.3 | 5.5 | 5.0 | 4.6 | 4.9 | 5.2 | 5.1 | 5.1 | 5.5 | 6.9 | 6.3 | 6.4 | 5.9 | 7.1 | 7.1 | 6.1 | 6.5 | 6.9 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.1 | 1.1 | 1.1 | 0.7 | 0.8 | 0.8 | 0.9 | 0.9 | 1.1 | 1.4 | 0.9 | 1.0 | 1.0 | 1.1 | 1.5 | 1.2 | 0.9 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.11 | -0.02 | -0.14 | -0.06 | 0.05 | 0.01 | 0.01 | 0.12 | 0.44 | 0.33 | 0.35 | 0.23 | 0.49 | 0.49 | 0.26 | 0.36 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 1.0 | 0 | 0.6 | 2.1 | 0.8 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | -0.1 | 2.2 | 0 | 0 | -0.2 | -0.1 | -1.0 | 1.3 | 5.1 | 4.2 | 4.2 | -0.6 | 0.6 | 1.6 |
Cytoplasm | 0 | -1.7 | -2.5 | -3.1 | -2.1 | -4.4 | -3.5 | -4.4 | -5.5 | -3.6 | -3.5 | -2.2 | -3.1 | -5.1 | -5.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.6 | 0 | 0 | 5.7 | 4.7 | 6.2 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.5391 | 6.6225 | 6.089 | 5.7286 | 5.8933 | 6.3404 | 3.573 | 4.5136 | 4.2146 | 3.8972 | 3.7564 | 4.1851 | 5.7488 | 6.7233 | 6.021 | 6.2242 | 5.4283 | 6.3104 |
Actin | 0.0378 | 0.0035 | 0.0098 | 0.0071 | 0.0121 | 0.0113 | 0.0484 | 0.0026 | 0.0235 | 0.0268 | 0.0232 | 0.0074 | 0.0578 | 0.0078 | 0.0129 | 0.0076 | 0.0382 | 0.0062 |
Bud | 0.0021 | 0.0004 | 0.001 | 0.0005 | 0.0003 | 0.0009 | 0.0014 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0012 | 0.0002 | 0.0002 | 0.0001 | 0.0012 | 0.0004 |
Bud Neck | 0.0068 | 0.0013 | 0.0048 | 0.0114 | 0.0048 | 0.021 | 0.0099 | 0.0015 | 0.0044 | 0.002 | 0.0077 | 0.008 | 0.0126 | 0.0018 | 0.0015 | 0.0022 | 0.002 | 0.0026 |
Bud Periphery | 0.0088 | 0.0013 | 0.0102 | 0.0055 | 0.0023 | 0.0077 | 0.007 | 0.0007 | 0.0028 | 0.0041 | 0.0034 | 0.0065 | 0.0064 | 0.0011 | 0.0023 | 0.0022 | 0.0036 | 0.0044 |
Bud Site | 0.0127 | 0.0258 | 0.0193 | 0.0037 | 0.0036 | 0.0036 | 0.0198 | 0.0295 | 0.0359 | 0.0027 | 0.001 | 0.0015 | 0.0158 | 0.0242 | 0.0045 | 0.0015 | 0.0053 | 0.0008 |
Cell Periphery | 0.3971 | 0.3872 | 0.4633 | 0.3836 | 0.4807 | 0.4942 | 0.5095 | 0.5376 | 0.4889 | 0.5681 | 0.5541 | 0.6601 | 0.5532 | 0.5426 | 0.5823 | 0.6915 | 0.5262 | 0.664 |
Cytoplasm | 0.2916 | 0.5355 | 0.4212 | 0.4897 | 0.43 | 0.4061 | 0.2736 | 0.3793 | 0.3897 | 0.3402 | 0.281 | 0.2745 | 0.2488 | 0.4036 | 0.3365 | 0.2675 | 0.3057 | 0.3002 |
Cytoplasmic Foci | 0.0234 | 0.0009 | 0.0038 | 0.001 | 0.0037 | 0.004 | 0.0218 | 0.0026 | 0.0035 | 0.0088 | 0.0053 | 0.0034 | 0.0128 | 0.0025 | 0.0078 | 0.0026 | 0.0107 | 0.0002 |
Eisosomes | 0.0064 | 0.0013 | 0.001 | 0.0037 | 0.0005 | 0.0021 | 0.0031 | 0.0021 | 0.002 | 0.0013 | 0.0025 | 0.0009 | 0.0155 | 0.0007 | 0.0071 | 0.0008 | 0.0008 | 0.0007 |
Endoplasmic Reticulum | 0.0148 | 0.0054 | 0.0076 | 0.0179 | 0.0035 | 0.0078 | 0.0123 | 0.0025 | 0.0033 | 0.0067 | 0.0079 | 0.0044 | 0.0117 | 0.0032 | 0.0075 | 0.0057 | 0.0042 | 0.0075 |
Endosome | 0.0448 | 0.0008 | 0.0136 | 0.0058 | 0.006 | 0.0064 | 0.0207 | 0.0001 | 0.0087 | 0.0066 | 0.0153 | 0.0014 | 0.0054 | 0.0005 | 0.0037 | 0.0024 | 0.008 | 0.0013 |
Golgi | 0.0126 | 0.0006 | 0.0045 | 0.007 | 0.0054 | 0.0078 | 0.0133 | 0.0004 | 0.0055 | 0.0107 | 0.0071 | 0.002 | 0.0052 | 0.0012 | 0.0027 | 0.0023 | 0.0051 | 0.0011 |
Lipid Particles | 0.0262 | 0.0011 | 0.0021 | 0.007 | 0.0082 | 0.0013 | 0.0166 | 0.0014 | 0.0017 | 0.0055 | 0.0091 | 0.0011 | 0.0125 | 0.0014 | 0.0065 | 0.0076 | 0.0069 | 0.0006 |
Mitochondria | 0.0274 | 0.0096 | 0.0139 | 0.0444 | 0.0045 | 0.0162 | 0.0037 | 0.0001 | 0.0013 | 0.0025 | 0.03 | 0.0013 | 0.0033 | 0.0001 | 0.0024 | 0.0008 | 0.0012 | 0.0012 |
None | 0.02 | 0.0221 | 0.0101 | 0.0019 | 0.028 | 0.005 | 0.0142 | 0.0254 | 0.0239 | 0.0046 | 0.0331 | 0.0245 | 0.0036 | 0.0014 | 0.0089 | 0.0008 | 0.0112 | 0.0012 |
Nuclear Periphery | 0.0094 | 0.0003 | 0.0038 | 0.0013 | 0.0003 | 0.0002 | 0.0036 | 0.0002 | 0.0004 | 0.0007 | 0.0053 | 0.0002 | 0.002 | 0.0002 | 0.0012 | 0.0003 | 0.0083 | 0.002 |
Nucleolus | 0.0033 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0033 | 0.0002 |
Nucleus | 0.0236 | 0.0003 | 0.0008 | 0.0004 | 0.0001 | 0.0003 | 0.0028 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0046 | 0.0001 | 0.0003 | 0.0001 | 0.0143 | 0.0029 |
Peroxisomes | 0.0065 | 0 | 0.0016 | 0.0003 | 0.0041 | 0.0003 | 0.008 | 0.0128 | 0.002 | 0.0056 | 0.0045 | 0.0003 | 0.0163 | 0.0066 | 0.0046 | 0.0021 | 0.0074 | 0 |
Punctate Nuclear | 0.0061 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0036 | 0.0001 | 0.0001 | 0.0003 | 0.0063 | 0.0001 | 0.0053 | 0.0002 | 0.0051 | 0.0005 | 0.0345 | 0.0003 |
Vacuole | 0.0159 | 0.0023 | 0.0063 | 0.0061 | 0.0014 | 0.0036 | 0.005 | 0.0007 | 0.0013 | 0.0022 | 0.0014 | 0.0016 | 0.0046 | 0.0008 | 0.0016 | 0.0014 | 0.0016 | 0.002 |
Vacuole Periphery | 0.0027 | 0.0001 | 0.0006 | 0.0013 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.2305 | 29.7061 | 33.5657 | 35.3846 | 29.376 | 27.1736 | 36.3501 | 35.2458 | 31.1444 | 36.2575 |
Translational Efficiency | 0.8765 | 0.9767 | 0.785 | 0.7889 | 0.7778 | 0.8998 | 0.7396 | 0.6061 | 0.8885 | 0.7274 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1030 | 1310 | 1518 | 1370 | 1253 | 2302 | 147 | 1089 | 2283 | 3612 | 1665 | 2459 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 709.37 | 762.49 | 1034.95 | 972.49 | 834.09 | 824.45 | 1117.93 | 975.39 | 777.82 | 801.98 | 1042.28 | 973.77 |
Standard Deviation | 83.70 | 129.44 | 121.88 | 115.53 | 101.01 | 109.06 | 125.02 | 113.34 | 112.30 | 120.60 | 124.41 | 114.57 |
Intensity Change Log 2 | — | 0.104180 | 0.544951 | 0.455145 | — | -0.016771 | 0.422555 | 0.225776 | — | 0.040079 | 0.480100 | 0.335737 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002589 | 0.015583 | 0.029142 | 0.065788 | 0.007004 | 0.018553 | 0.051276 | 0.053399 | 0.005012 | 0.017476 | 0.031096 | 0.060302 |
Bud Neck | 0.053358 | 0.045247 | 0.029770 | 0.040775 | 0.033732 | 0.064062 | 0.019592 | 0.022850 | 0.042587 | 0.057238 | 0.028872 | 0.032837 |
Bud Site | 0.037492 | 0.116627* | 0.173655* | 0.185094* | 0.079599 | 0.088755 | 0.179640* | 0.141536* | 0.060602 | 0.098863 | 0.174183* | 0.165804* |
Cell Periphery | 0.048261 | 0.029281 | 0.121307* | 0.098215 | 0.099403 | 0.039418 | 0.082730 | 0.059755 | 0.076330 | 0.035742 | 0.117901* | 0.081183 |
Cytoplasm | 0.606574* | 0.542965* | 0.351016* | 0.370917* | 0.654663* | 0.605260* | 0.222775* | 0.415517* | 0.632967* | 0.582666* | 0.339694* | 0.390669* |
Cytoplasmic Foci | 0.033242 | 0.097367 | 0.015992 | 0.009100 | 0.029121 | 0.041601 | 0.051836 | 0.025291 | 0.030980 | 0.061826 | 0.019156 | 0.016270 |
Eisosomes | 0.029874 | 0.011802 | 0.014947 | 0.035884 | 0.021914 | 0.031490 | 0.017856 | 0.028197 | 0.025505 | 0.024350 | 0.015204 | 0.032480 |
Endoplasmic Reticulum | 0.005504 | 0.010072 | 0.082624 | 0.010829 | 0.009884 | 0.005348 | 0.060459 | 0.010458 | 0.007908 | 0.007062 | 0.080667 | 0.010665 |
Endosome | 0.001336 | 0.014660 | 0.001697 | 0.003267 | 0.002116 | 0.005106 | 0.003434 | 0.003281 | 0.001764 | 0.008571 | 0.001851 | 0.003273 |
Golgi | 0.004162 | 0.010840 | 0.002198 | 0.003367 | 0.008271 | 0.010112 | 0.001887 | 0.002305 | 0.006417 | 0.010376 | 0.002171 | 0.002896 |
Lipid Particles | 0.004293 | 0.008656 | 0.006277 | 0.002199 | 0.003578 | 0.005439 | 0.009231 | 0.002989 | 0.003901 | 0.006605 | 0.006538 | 0.002549 |
Mitochondria | 0.002607 | 0.003845 | 0.000833 | 0.002783 | 0.001859 | 0.004576 | 0.000122 | 0.000711 | 0.002197 | 0.004311 | 0.000770 | 0.001865 |
Mitotic Spindle | 0.000227 | 0.000192 | 0.000341 | 0.003573 | 0.000200 | 0.000210 | 0.000311 | 0.003934 | 0.000212 | 0.000203 | 0.000338 | 0.003733 |
None | 0.005792 | 0.002794 | 0.001206 | 0.000513 | 0.001593 | 0.001492 | 0.000163 | 0.000302 | 0.003487 | 0.001964 | 0.001114 | 0.000419 |
Nuclear Periphery | 0.000169 | 0.000141 | 0.000287 | 0.000113 | 0.000085 | 0.000070 | 0.000210 | 0.000114 | 0.000123 | 0.000096 | 0.000280 | 0.000114 |
Nuclear Periphery Foci | 0.000491 | 0.000784 | 0.003405 | 0.000876 | 0.001243 | 0.000382 | 0.008384 | 0.001782 | 0.000903 | 0.000528 | 0.003844 | 0.001277 |
Nucleolus | 0.000292 | 0.000318 | 0.000077 | 0.000054 | 0.000152 | 0.000174 | 0.000037 | 0.000038 | 0.000216 | 0.000226 | 0.000073 | 0.000047 |
Nucleus | 0.142554* | 0.029051 | 0.112069 | 0.063143 | 0.023898 | 0.039238 | 0.071821 | 0.049885 | 0.077431 | 0.035543 | 0.108516 | 0.057271 |
Peroxisomes | 0.002939 | 0.005592 | 0.000804 | 0.001321 | 0.002719 | 0.005955 | 0.001279 | 0.002703 | 0.002819 | 0.005823 | 0.000846 | 0.001933 |
Vacuole | 0.018000 | 0.052713 | 0.052217 | 0.100749 | 0.018516 | 0.032189 | 0.216866* | 0.174695 | 0.018283 | 0.039633 | 0.066753 | 0.133497 |
Vacuole Periphery | 0.000241 | 0.001471 | 0.000136 | 0.001441 | 0.000450 | 0.000571 | 0.000092 | 0.000258 | 0.000356 | 0.000897 | 0.000132 | 0.000917 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -12.06 | -19.34 | -22.53 | -16.56 | -11.38 | -11.83 | -8.42 | -14.51 | -10.15 | -0.03 | -17.12* | -19.32 | -26.21 | -18.97 | -11.05 |
Bud Neck | 2.28 | 7.79 | 4.19 | 2.11 | -4.34 | -10.73 | 5.86 | 5.79 | 17.75 | -0.99 | -6.38 | 7.27 | 5.65 | 13.21 | -1.51 |
Bud Site | -16.06 | -24.76* | -22.56 | -8.97 | -0.82 | -2.16 | -6.61 | -8.27 | -7.31 | 2.69 | -12.04 | -22.34 | -21.27 | -13.08 | 2.35 |
Cell Periphery | 5.57 | -14.88 | -9.15 | -14.35 | 4.92 | 13.98 | 2.43 | 9.01 | -4.21 | 2.33 | 14.21 | -8.68 | 0.38 | -13.78 | 9.00 |
Cytoplasm | 7.51 | 30.34 | 27.04 | 19.48 | -1.79 | 6.82 | 28.06 | 26.17 | 22.55 | -11.69 | 9.14 | 41.53 | 38.26 | 31.93 | -6.15 |
Cytoplasmic Foci | -15.07 | 8.82 | 12.29 | 22.24 | 5.72 | -5.82 | -3.69 | 1.49 | 6.80 | 4.16 | -14.54 | 8.26 | 10.49 | 22.45 | 1.84 |
Eisosomes | 11.18 | 10.09 | -1.68 | -11.30 | -10.30 | -6.17 | 2.28 | -1.80 | 3.00 | -3.28 | 1.00 | 10.51 | -3.02 | -3.85 | -11.29 |
Endoplasmic Reticulum | -3.32 | -21.27 | -4.80 | -0.63 | 19.76 | 4.65 | -7.95 | -0.05 | -4.30 | 7.92 | 1.08 | -21.83 | -2.99 | -4.33 | 21.03 |
Endosome | -11.33 | -2.02 | -2.28 | 8.40 | -1.84 | -5.18 | -3.20 | -1.15 | 2.08 | 0.61 | -12.03 | -0.63 | -2.32 | 7.10 | -2.17 |
Golgi | -9.35 | 6.84 | 1.19 | 7.21 | -1.14 | -2.78 | 17.45 | 8.20 | 8.79 | -0.43 | -8.02 | 18.44 | 6.30 | 10.76 | -0.94 |
Lipid Particles | -5.56 | -5.66 | 7.96 | 8.17 | 11.98 | -5.40 | -5.55 | 2.99 | 7.12 | 6.14 | -7.49 | -8.69 | 8.04 | 11.19 | 13.06 |
Mitochondria | -2.30 | 9.49 | 0.02 | 1.16 | -1.83 | -6.97 | 12.88 | 5.38 | 9.60 | -2.82 | -6.68 | 12.39 | 0.85 | 4.19 | -1.77 |
Mitotic Spindle | 0.46 | -1.44 | -2.86 | -2.89 | -2.76 | -0.21 | -1.10 | -2.79 | -2.78 | -1.40 | 0.22 | -2.14 | -3.99 | -4.01 | -3.71 |
None | 2.21 | 4.47 | 5.10 | 2.50 | 2.96 | 0.16 | 3.76 | 3.34 | 2.84 | -1.29 | 2.31 | 4.55 | 5.94 | 3.60 | 4.00 |
Nuclear Periphery | 0.81 | -7.62 | 1.48 | 0.25 | 5.86 | 1.28 | -6.33 | -2.61 | -3.16 | 4.14 | 1.88 | -11.64 | -0.37 | -1.65 | 8.13 |
Nuclear Periphery Foci | -2.68 | -17.02 | -4.36 | -1.47 | 14.00 | 2.22 | -6.35 | -1.01 | -10.98 | 6.38 | 1.79 | -9.97 | -1.48 | -10.05 | 13.58 |
Nucleolus | -0.47 | 7.37 | 7.72 | 3.37 | 2.13 | -0.16 | 2.15 | 2.17 | 17.41 | 0.17 | -0.20 | 4.31 | 4.99 | 5.99 | 4.36 |
Nucleus | 22.36 | 4.34 | 13.48 | -12.40 | 10.64 | -8.66 | -7.46 | -11.10 | -4.62 | 3.36 | 15.04 | -8.22 | 5.44 | -11.96 | 13.59 |
Peroxisomes | -5.85 | 7.49 | 5.22 | 11.30 | -3.19 | -10.04 | 2.90 | 0.35 | 5.13 | -1.56 | -11.09 | 12.78 | 3.18 | 11.11 | -3.44 |
Vacuole | -13.69 | -18.60 | -21.99 | -13.27 | -13.54 | -9.20 | -13.58 | -27.25 | -25.12 | 1.86 | -16.20 | -20.60 | -34.28 | -28.32 | -18.47 |
Vacuole Periphery | -5.58 | 4.51 | -2.32 | 0.25 | -2.55 | -2.38 | 11.10 | 2.57 | 3.85 | -1.90 | -6.28 | 10.75 | -1.86 | 0.16 | -2.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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