Standard name
Human Ortholog
Description Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.97 0.89 0.89 0.89 0.86 0.74 0.62 0.58 – 0.52 0.4 0.39 0.99 1.0 0.98 – – – 0.75 0.75 0.53 0.39 0.44 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.1 0.13 0.18 0.18 0.4 0.58 0.59 – 0.67 0.74 0.78 0 0 0 – – – 0.1 0.12 0.31 0.38 0.32 0.27
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 – – – 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0.1 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 – – – 5 1 1 2 6 4
Bud 1 1 1 2 1 2 3 9 – 6 3 6 0 0 1 – – – 5 1 0 0 3 8
Bud Neck 2 0 1 0 0 2 0 0 – 2 0 0 3 0 3 – – – 3 0 1 0 7 10
Bud Site 0 0 0 0 0 2 1 6 – 2 3 2 0 0 0 – – – – – – – – –
Cell Periphery 1 1 0 1 1 0 0 1 – 1 0 0 1 0 0 – – – 1 0 0 0 3 2
Cytoplasm 368 110 81 83 96 137 82 147 – 79 63 70 287 267 386 – – – 314 96 57 45 123 118
Endoplasmic Reticulum 1 2 0 0 0 0 0 1 – 0 0 1 1 1 0 – – – 5 0 1 2 4 2
Endosome 2 2 0 0 3 0 0 0 – 0 1 1 0 0 1 – – – 10 3 2 0 4 5
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 1 0 0 – – – 0 1 0 1 0 0
Mitochondria 6 12 12 17 20 74 76 151 – 103 116 139 0 0 0 – – – 40 15 33 45 88 74
Nucleus 0 1 1 0 1 2 0 5 – 0 0 1 1 0 0 – – – 0 0 0 0 0 1
Nuclear Periphery 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 2 0
Nucleolus 2 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 – 0 0 0 1 0 0 – – – 0 0 0 0 0 0
SpindlePole 2 2 0 1 0 5 5 6 – 6 6 11 0 0 3 – – – 8 1 5 1 7 6
Vac/Vac Membrane 6 1 0 1 2 0 2 2 – 1 2 2 1 0 3 – – – 10 2 1 12 21 36
Unique Cell Count 381 124 91 93 111 184 132 255 153 157 179 291 268 393 422 128 108 118 278 282
Labelled Cell Count 393 133 96 105 125 224 169 329 200 194 233 296 268 398 422 128 108 118 278 282


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.0 3.2 3.4 4.0 3.6 2.9 3.2 – 2.7 3.1 2.8 5.2 4.8 4.9 – – – 5.9 5.4 5.5
Std Deviation (1e-4) 0.8 0.9 0.6 1.1 2.5 2.1 1.2 1.9 – 1.1 2.0 1.7 1.1 1.0 1.3 – – – 2.1 1.5 1.6
Intensity Change (Log2) – – – 0.06 0.29 0.13 -0.18 -0.01 – -0.25 -0.07 -0.2 0.67 0.57 0.6 – – – 0.87 0.74 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7772 -0.258 -0.5436 -0.6338 -0.7395 -0.4008 1.8205 1.5972 1.999 2.5396 2.2949 2.2469 -0.6672 -0.6296 -0.4695 -0.4251 0.7137 -0.4815
Actin 0.001 0.0012 0.0007 0.0003 0.0001 0.0004 0.0457 0.0008 0.0214 0.0025 0.0166 0.0036 0.0486 0 0.0364 0.0648 0.0543 0.0002
Bud 0.0002 0.0002 0.0033 0.0001 0.0001 0.0006 0.0043 0.0011 0.0003 0.0004 0.0108 0.0004 0 0 0.0019 0.0001 0.0012 0
Bud Neck 0.0021 0.0002 0.0002 0.0001 0.0001 0.0051 0.0022 0 0.0002 0.0008 0.0061 0.0004 0.0007 0 0.0014 0.0002 0.0006 0.0001
Bud Periphery 0.0002 0.0003 0.0031 0.0003 0.0001 0.0005 0.0123 0.0074 0.0012 0.0008 0.0248 0.0019 0.0001 0 0.0032 0.0001 0.0008 0
Bud Site 0.0031 0.0025 0.0051 0.0001 0 0.0002 0.0023 0.0004 0.0004 0.0006 0.0214 0.0001 0.0002 0.0002 0.0062 0.0001 0.0017 0
Cell Periphery 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0014 0.001 0.0006 0.0001 0.0011 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0
Cytoplasm 0.0474 0.0658 0.0531 0.1155 0.0634 0.0801 0.0723 0.0761 0.0828 0.186 0.1094 0.1606 0.0546 0.1082 0.0776 0.0984 0.059 0.1458
Cytoplasmic Foci 0.0157 0.0086 0.0118 0.0054 0.0062 0.0121 0.0093 0.0084 0.0078 0.042 0.0304 0.0054 0.0053 0.002 0.0159 0.0042 0.0351 0.0022
Eisosomes 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0005 0.0002 0.0006 0.0012 0.0005 0.0002 0.0005 0.0003 0.0004 0.0001
Endoplasmic Reticulum 0.0008 0.0011 0.0007 0.0015 0.0005 0.0009 0.0104 0.0111 0.0209 0.0008 0.0485 0.0268 0.0023 0.0013 0.0023 0.0015 0.0071 0.0011
Endosome 0.0017 0.0019 0.001 0.0011 0.0027 0.0033 0.0067 0.0039 0.0028 0.0111 0.0221 0.0012 0.0013 0.0008 0.0099 0.0013 0.0394 0.0004
Golgi 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002 0.0012 0.0004 0.0007 0.0065 0.0041 0.0003 0.0005 0 0.0112 0.0026 0.0183 0
Lipid Particles 0.0015 0.0004 0.0005 0.0002 0.0002 0.0012 0.0022 0.0008 0.0006 0.0135 0.0025 0.0005 0.0011 0.0002 0.0034 0.0014 0.0758 0.0001
Mitochondria 0.0003 0.0007 0.0006 0.0011 0.0004 0.0005 0.0011 0.0034 0.0007 0.006 0.0105 0.0009 0.0003 0 0.0043 0.0004 0.0049 0.0001
None 0.9178 0.9091 0.9122 0.871 0.9228 0.8856 0.8107 0.8762 0.8535 0.7006 0.6638 0.7936 0.8804 0.8863 0.7862 0.8195 0.627 0.8492
Nuclear Periphery 0.0006 0.0007 0.0005 0.0005 0.0004 0.0004 0.0016 0.0048 0.0012 0.0003 0.0084 0.0008 0.001 0.0001 0.0066 0.0003 0.0202 0.0001
Nucleolus 0.0004 0.0004 0.0004 0.0001 0.0001 0.0008 0.0005 0.0001 0.0002 0.0002 0.0021 0.0003 0.0003 0 0.0005 0.0001 0.0005 0
Nucleus 0.0012 0.0019 0.0013 0.0008 0.0005 0.0012 0.0012 0.0014 0.0007 0.0004 0.0053 0.0006 0.0009 0.0001 0.0251 0.0003 0.0085 0.0002
Peroxisomes 0.0011 0.0006 0.0007 0.0004 0.0001 0.0005 0.0102 0.0004 0.0004 0.0251 0.0031 0.0003 0.0002 0 0.0029 0.0033 0.0276 0
Punctate Nuclear 0.0034 0.0028 0.0035 0.0006 0.0006 0.0055 0.0015 0.0003 0.001 0.0008 0.0046 0.0005 0.0008 0.0001 0.0023 0.0004 0.0143 0.0001
Vacuole 0.0006 0.0009 0.0006 0.0004 0.0013 0.0005 0.0024 0.0013 0.0019 0.001 0.0024 0.0004 0.0007 0.0002 0.0012 0.0005 0.0018 0.0002
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0006 0.0003 0.0002 0.0014 0.0001 0.0001 0 0.0005 0.0001 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 97.8827 116.6681 59.0269 102.8619 103.8347 47.432 100.5367 166.4721 121.8448 155.1717
Translational Efficiency 3.4799 2.2007 4.1576 1.3162 2.4609 9.9655 3.5645 2.6307 3.1491 2.4526

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1522 1759 133 1180 1456 1351 1414 896 2978 3110 1547 2076

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.29 729.54 812.32 795.89 606.51 673.11 814.79 833.97 623.77 705.03 814.58 812.33
Standard Deviation 73.51 128.52 80.72 108.83 95.44 80.00 90.55 109.28 86.61 113.60 89.75 110.64
Intensity Change Log 2 — 0.188262 0.343323 0.313844 — 0.150311 0.425897 0.459464 — 0.169925 0.384082 0.386518

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000027 0.000192 0.000296 0.000443 0.000181 0.000329 0.000073 0.000461 0.000102 0.000251 0.000092 0.000450
Bud Neck 0.004824 0.038039 0.001594 0.007578 0.018677 0.061083 0.004173 0.011760 0.011597 0.048049 0.003951 0.009383
Bud Site 0.001216 0.011568 0.002031 0.023059 0.002480 0.016339 0.007633 0.025607 0.001834 0.013640 0.007151 0.024159
Cell Periphery 0.000136 0.000249 0.000294 0.000152 0.000164 0.000172 0.000081 0.000237 0.000150 0.000216 0.000099 0.000189
Cytoplasm 0.598261* 0.418855* 0.267512* 0.488242* 0.444807* 0.317702* 0.505664* 0.447762* 0.523235* 0.374914* 0.485189* 0.470771*
Cytoplasmic Foci 0.160654 0.234021* 0.049556 0.028024 0.221429* 0.278482* 0.013405 0.040928 0.190368* 0.253335* 0.016513 0.033593
Eisosomes 0.000090 0.000113 0.000125 0.000057 0.000237 0.000191 0.000026 0.000076 0.000162 0.000147 0.000034 0.000065
Endoplasmic Reticulum 0.000361 0.000848 0.004203 0.001543 0.000973 0.000817 0.000958 0.002412 0.000660 0.000834 0.001237 0.001918
Endosome 0.004208 0.023453 0.006934 0.005275 0.016253 0.043017 0.001536 0.012591 0.010097 0.031952 0.002000 0.008433
Golgi 0.000892 0.004967 0.000195 0.001356 0.001648 0.008764 0.000089 0.004657 0.001262 0.006617 0.000098 0.002781
Lipid Particles 0.005654 0.009233 0.006287 0.001641 0.020255 0.011847 0.000844 0.002100 0.012792 0.010368 0.001312 0.001839
Mitochondria 0.001936 0.012238 0.000459 0.005731 0.004753 0.035561 0.000739 0.002894 0.003313 0.022370 0.000715 0.004507
Mitotic Spindle 0.000048 0.005060 0.003807 0.022494 0.000959 0.007455 0.007432 0.023472 0.000494 0.006100 0.007120 0.022916
None 0.011446 0.006898 0.004407 0.004976 0.010291 0.001699 0.004981 0.008327 0.010881 0.004639 0.004931 0.006423
Nuclear Periphery 0.000367 0.000593 0.001815 0.001477 0.000750 0.000533 0.001337 0.001285 0.000554 0.000567 0.001378 0.001394
Nuclear Periphery Foci 0.000249 0.000653 0.002355 0.001093 0.000395 0.000198 0.000354 0.000790 0.000321 0.000455 0.000526 0.000962
Nucleolus 0.001207 0.002858 0.001932 0.001204 0.003127 0.002276 0.000408 0.001866 0.002146 0.002605 0.000539 0.001490
Nucleus 0.187428* 0.149155* 0.576601* 0.277490* 0.194176* 0.094936 0.409605* 0.253520* 0.190727* 0.125602 0.423962* 0.267145*
Peroxisomes 0.001219 0.010761 0.000904 0.001728 0.005244 0.020922 0.000197 0.001559 0.003187 0.015175 0.000258 0.001655
Vacuole 0.019128 0.067325 0.068260 0.122636 0.052131 0.093205 0.040127 0.153849 0.035264 0.078567 0.042546 0.136107
Vacuole Periphery 0.000646 0.002923 0.000435 0.003802 0.001070 0.004472 0.000339 0.003846 0.000853 0.003596 0.000347 0.003821

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.61 -6.12 -4.50 -2.12 0.41 -1.03 0.55 -1.28 -0.37 -3.97 -2.12 -0.42 -3.12 -1.74 -4.51
Bud Neck -14.19 4.20 -1.83 13.16 -5.82 -11.72 7.57 4.96 15.50 -2.42 -17.37 6.43 3.73 20.22 -3.05
Bud Site -10.95 -2.36 -6.14 -1.11 -5.55 -9.91 -2.78 -5.61 -0.21 -3.87 -14.41 -3.52 -8.25 -0.95 -4.87
Cell Periphery -2.33 -5.96 2.60 2.74 6.61 -0.35 10.60 0.08 0.31 -3.98 -2.27 8.63 1.32 2.63 -3.70
Cytoplasm 20.66 18.79 21.00 2.72 -7.29 12.74 -0.25 4.34 -6.38 4.33 21.90 10.80 17.14 -2.11 3.95
Cytoplasmic Foci -10.91 15.29 31.50 40.42 3.85 -7.16 41.48 34.90 38.14 -9.40 -12.08 50.97 46.83 55.31 -7.76
Eisosomes -4.68 -4.16 6.56 10.54 6.34 2.36 12.68 9.98 9.71 -9.81 1.58 14.42 11.21 13.29 -8.94
Endoplasmic Reticulum -5.35 -7.63 -13.75 -10.97 4.30 0.61 -3.75 -3.82 -4.03 -2.29 -1.46 -9.93 -9.70 -8.66 -2.93
Endosome -14.12 -3.24 1.34 14.56 3.92 -9.90 13.31 5.25 12.91 -5.02 -15.22 13.52 5.93 18.66 -5.59
Golgi -9.57 5.89 1.26 9.27 -1.57 -7.54 6.24 -0.63 4.75 -2.22 -11.17 8.62 -0.10 8.17 -2.72
Lipid Particles -3.68 -0.92 7.81 9.34 6.15 4.81 13.28 12.56 9.94 -6.23 2.54 14.74 14.31 13.61 -2.15
Mitochondria -10.34 4.55 -0.55 7.07 -2.07 -10.96 4.95 3.38 12.50 -2.33 -13.90 5.98 1.61 14.17 -2.49
Mitotic Spindle -5.35 -1.03 -6.49 -4.12 -3.54 -3.78 -3.62 -4.83 -2.61 -2.80 -6.12 -4.23 -7.97 -4.76 -4.18
None 3.16 6.47 6.90 2.02 -0.54 5.46 3.54 2.38 -3.21 -0.79 5.70 6.49 5.54 -0.45 -0.57
Nuclear Periphery -2.16 -10.42 -16.90 -16.75 -3.23 1.64 -14.89 -10.72 -8.86 -0.15 -0.21 -18.01 -20.07 -18.21 -4.83
Nuclear Periphery Foci -2.09 -6.09 -10.30 -2.96 3.38 3.10 -1.87 -7.15 -8.66 -5.83 -1.35 -6.47 -12.34 -5.30 -5.87
Nucleolus -3.84 -2.13 2.09 4.61 3.32 2.18 6.33 3.87 2.75 -2.09 -1.08 7.13 4.53 5.37 -2.08
Nucleus 6.38 -16.78 -13.55 -18.18 11.54 16.23 -27.10 -7.99 -19.18 14.82 15.01 -32.51 -15.36 -26.17 17.84
Peroxisomes -12.37 1.45 1.12 11.82 0.29 -9.35 6.56 5.14 13.15 -3.25 -13.76 7.67 5.09 17.48 -2.87
Vacuole -18.64 -7.15 -27.89 -20.25 -10.70 -9.55 -4.80 -20.21 -14.61 -16.80 -17.91 -10.48 -33.80 -24.79 -23.21
Vacuole Periphery -6.87 1.59 -1.30 1.86 -1.65 -9.61 5.15 -1.51 3.62 -2.84 -11.34 4.22 -1.87 3.61 -3.08
Standard name
Human Ortholog
Description Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tom6

Tom6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tom6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available