Standard name
Human Ortholog
Description Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.18 0.15 0.12 0.1 0.14 0.09 0.09 0.07 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.75 0.72 0.69 0.74 0.76 0.77 0.78 0.77 0.74 0.64 0.59 0.91 0.96 0.97 0.67 0.66 0.64 0.49 0.46 0.41 0.55 0.68 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0 0 0 0.15 0.11 0.08 0.29 0.23 0.22 0.24 0 0 0 0.05 0 0 0.05 0.06 0.07 0.2 0.14 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0.1 0.11 0.19 0.21 0.07 0.14 0.13 0 0.05 0.1 0.14 0 0 0 0.21 0.22 0.28 0.27 0.27 0.33 0.07 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 0 0 1 2 0 0 0 1 0 0 0 1 0 0 2 0 1 1 5 10
Bud 2 2 4 1 1 2 8 6 5 5 4 3 5 0 0 1 3 0 6 8 4 6 13 11
Bud Neck 12 7 15 4 10 7 26 19 14 12 11 10 2 0 1 8 7 8 8 9 3 6 4 8
Bud Site 8 4 2 1 1 1 2 0 0 1 0 4 0 0 0 2 2 2 – – – – – –
Cell Periphery 5 0 0 0 0 0 0 1 1 0 1 0 0 0 0 5 1 0 0 1 1 2 4 8
Cytoplasm 244 195 59 18 59 52 138 167 122 122 52 46 249 346 366 163 192 161 165 133 43 138 252 332
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 9 9 13 0 0 0 3 5 3
Endosome 4 7 0 0 0 0 0 0 0 0 0 0 6 8 4 13 15 16 13 11 0 3 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 1 0 0 2 1
Mitochondria 9 38 2 0 1 10 20 18 46 37 18 19 8 1 4 11 12 4 17 17 6 50 53 49
Nucleus 5 5 2 1 2 2 3 3 4 4 2 1 0 1 0 4 6 0 1 3 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 0 0 0 0 0 1 0 1 0
Nucleolus 6 6 1 0 0 2 0 0 1 3 1 1 0 0 0 18 9 7 8 9 1 1 0 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 3 5 3 3 4 8 1 0 0 1 0 1
SpindlePole 43 27 9 5 17 5 26 27 6 8 8 11 6 8 5 51 63 69 90 77 34 18 3 6
Vac/Vac Membrane 3 3 0 1 0 0 0 0 1 0 0 0 1 2 1 11 9 3 8 7 1 7 16 15
Unique Cell Count 318 261 82 26 80 68 180 213 158 164 81 78 273 361 376 242 289 250 339 293 106 252 373 463
Labelled Cell Count 345 294 95 31 91 81 224 244 200 192 97 96 281 372 386 300 334 292 339 293 106 252 373 463


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 4.3 4.2 4.6 3.5 3.6 3.7 3.0 3.1 3.1 3.3 3.8 4.2 4.1 5.9 5.9 5.9 3.6 4.0 4.1
Std Deviation (1e-4) 1.0 0.6 1.2 0.8 1.8 1.3 1.0 1.2 1.0 1.0 1.5 1.1 1.4 2.4 1.8 1.5 1.6 1.7 1.1 1.9 1.9
Intensity Change (Log2) – – – -0.05 0.1 -0.29 -0.28 -0.22 -0.52 -0.51 -0.47 -0.39 -0.21 -0.05 -0.08 0.44 0.44 0.45 -0.26 -0.14 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3093 1.7226 1.4438 1.5516 1.8063 1.7596 -0.7803 -0.6915 -0.5077 -0.7725 -0.1549 -0.578 -0.7811 -0.2042 -0.4341 0.3541 0.2478 -0.3278
Actin 0.0488 0.0001 0.0107 0.0005 0.0391 0.0003 0.0551 0.0009 0.0105 0 0.04 0.0066 0.0122 0.0005 0.0059 0.0006 0.0056 0.006
Bud 0.001 0.0004 0.003 0.0004 0.0011 0.0016 0.0057 0.0136 0.0011 0 0.0035 0.0008 0.0067 0.0219 0.0106 0.0017 0.0006 0.0029
Bud Neck 0.0073 0.0008 0.0107 0.0251 0.0823 0.0586 0.001 0.0015 0.0007 0.0017 0.1024 0.0417 0.0027 0.002 0.0029 0.0382 0.1612 0.0526
Bud Periphery 0.0021 0.0006 0.0048 0.0008 0.0021 0.0017 0.0068 0.012 0.0022 0 0.0058 0.0013 0.0055 0.0072 0.0057 0.0018 0.0007 0.0017
Bud Site 0.0114 0.0039 0.0035 0.0008 0.0066 0.0005 0.0081 0.0256 0.0023 0.0001 0.004 0.0007 0.0131 0.0369 0.0227 0.0073 0.0059 0.0004
Cell Periphery 0.0008 0.0001 0.0001 0.0001 0.0002 0.0003 0.0004 0.001 0.0003 0 0.0006 0.0002 0.0007 0.0007 0.0004 0.0001 0.0003 0.0002
Cytoplasm 0.0124 0.0263 0.0247 0.0065 0.0013 0.0429 0.0908 0.0972 0.0564 0.0003 0.0029 0.1283 0.0304 0.0608 0.0275 0.0026 0.0048 0.0871
Cytoplasmic Foci 0.1163 0.0443 0.1107 0.0225 0.135 0.0624 0.1188 0.0713 0.2079 0.121 0.1127 0.1426 0.1434 0.0554 0.0912 0.0622 0.0958 0.1065
Eisosomes 0.0025 0.0001 0.0003 0.0001 0.0004 0.0001 0.0005 0.0001 0.0001 0 0.0003 0.0001 0.0006 0.0002 0.0002 0 0.001 0.0001
Endoplasmic Reticulum 0.0035 0.0004 0.0013 0.0003 0.0036 0.0005 0.0039 0.0016 0.0024 0 0.0011 0.0012 0.0043 0.0024 0.0012 0.0001 0.0024 0.0026
Endosome 0.013 0.0032 0.004 0.0016 0.0216 0.0039 0.0292 0.0102 0.0208 0.0003 0.0147 0.0101 0.0081 0.0052 0.0033 0.0006 0.0144 0.0059
Golgi 0.0139 0.0003 0.004 0.0004 0.0105 0.0009 0.0149 0.0009 0.0148 0.0003 0.0311 0.0034 0.003 0.0005 0.0008 0.0004 0.0032 0.0016
Lipid Particles 0.0197 0.0056 0.0103 0.0026 0.0109 0.0385 0.0309 0.0047 0.0267 0.0012 0.0088 0.0396 0.0139 0.0066 0.0034 0.0019 0.0194 0.0258
Mitochondria 0.0065 0.0001 0.0025 0.0006 0.0076 0.0075 0.0169 0.0013 0.0107 0 0.0379 0.0172 0.0032 0.001 0.0013 0.0002 0.0079 0.0037
None 0.62 0.7348 0.4397 0.0789 0.0034 0.6707 0.5337 0.5326 0.2464 0.0006 0.0105 0.4669 0.5808 0.5759 0.3183 0.1228 0.0279 0.5347
Nuclear Periphery 0.0034 0.0006 0.0013 0.0013 0.0251 0.0029 0.0047 0.0019 0.0022 0.0004 0.0005 0.0025 0.0041 0.0036 0.0032 0.0006 0.0077 0.0059
Nucleolus 0.0041 0.0134 0.0106 0.0284 0.0248 0.0138 0.0006 0.0261 0.002 0.0039 0.0473 0.0066 0.0093 0.0079 0.0077 0.0043 0.0446 0.0027
Nucleus 0.0032 0.0178 0.0249 0.0216 0.0052 0.0429 0.0125 0.0434 0.0033 0.0013 0.01 0.0417 0.0075 0.0194 0.023 0.0039 0.0185 0.0241
Peroxisomes 0.0234 0.0008 0.0133 0.0006 0.0218 0.011 0.0374 0.0027 0.0125 0.0014 0.0031 0.0202 0.0085 0.0014 0.002 0.001 0.0075 0.0125
Punctate Nuclear 0.081 0.1458 0.3187 0.8065 0.5926 0.0373 0.0099 0.148 0.3747 0.8675 0.5584 0.065 0.1393 0.1877 0.4675 0.7495 0.5669 0.1213
Vacuole 0.0042 0.0005 0.0005 0.0005 0.0017 0.0013 0.0094 0.003 0.0014 0 0.0035 0.0022 0.0022 0.0025 0.001 0.0003 0.0022 0.0012
Vacuole Periphery 0.0015 0.0001 0.0003 0.0001 0.0027 0.0004 0.0087 0.0003 0.0005 0 0.0008 0.0009 0.0005 0.0003 0.0002 0 0.0017 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6042 26.719 23.6818 22.974 7.2661 8.7128 30.9553 35.8342 25.6453 11.2337
Translational Efficiency 0.4348 0.606 0.7927 0.6011 0.5163 0.23 0.6281 0.6337 0.6557 0.6119

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
196 1366 1507 1567 1760 1523 205 53 1956 2889 1712 1620

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.83 700.26 872.19 900.22 609.12 724.28 916.33 868.54 611.80 712.92 877.48 899.18
Standard Deviation 67.90 116.71 95.94 131.11 89.21 93.31 83.91 88.60 87.68 105.71 95.66 130.06
Intensity Change Log 2 — 0.139250 0.456001 0.501637 — 0.249821 0.589141 0.511866 — 0.194408 0.522678 0.506651

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000282 0.000544 0.000450 0.000754 0.000123 0.001092 0.000505 0.001374 0.000139 0.000833 0.000457 0.000774
Bud Neck 0.043796 0.044495 0.038551 0.059513 0.039223 0.046557 0.026216 0.060747 0.039681 0.045582 0.037074 0.059553
Bud Site 0.024486 0.044190 0.047687 0.114904* 0.014369 0.036239 0.019851 0.158943* 0.015383 0.039999 0.044354 0.116345*
Cell Periphery 0.000306 0.000264 0.000190 0.000162 0.000264 0.000400 0.000155 0.000164 0.000268 0.000335 0.000186 0.000162
Cytoplasm 0.392355* 0.310200* 0.133947* 0.035698 0.402538* 0.255801* 0.058129 0.117927* 0.401518* 0.281522* 0.124869* 0.038389
Cytoplasmic Foci 0.276725* 0.277025* 0.008466 0.019083 0.234701* 0.344741* 0.018082 0.032555 0.238912* 0.312723* 0.009617 0.019523
Eisosomes 0.000299 0.000139 0.000044 0.000061 0.000173 0.000299 0.000121 0.000094 0.000186 0.000223 0.000053 0.000062
Endoplasmic Reticulum 0.000430 0.000659 0.003969 0.000630 0.000723 0.000471 0.003727 0.001467 0.000694 0.000560 0.003940 0.000657
Endosome 0.019559 0.029957 0.003984 0.006046 0.013100 0.029410 0.004081 0.022006 0.013747 0.029668 0.003996 0.006568
Golgi 0.002496 0.012144 0.000540 0.002975 0.002979 0.017947 0.000569 0.001183 0.002931 0.015204 0.000543 0.002916
Lipid Particles 0.007623 0.011984 0.001314 0.000896 0.013688 0.013211 0.003690 0.005489 0.013080 0.012631 0.001599 0.001047
Mitochondria 0.005525 0.019550 0.002729 0.006447 0.006536 0.023720 0.000493 0.002695 0.006435 0.021748 0.002461 0.006325
Mitotic Spindle 0.018978 0.076278 0.168401* 0.684505* 0.024066 0.075097 0.353842* 0.399720* 0.023556 0.075655 0.190607* 0.675188*
None 0.007334 0.010179 0.004382 0.000921 0.035643 0.008208 0.003209 0.001104 0.032806 0.009140 0.004242 0.000927
Nuclear Periphery 0.000303 0.000411 0.002274 0.000658 0.000863 0.000458 0.001451 0.000918 0.000807 0.000436 0.002175 0.000667
Nuclear Periphery Foci 0.001581 0.003014 0.002270 0.001520 0.003266 0.001950 0.003421 0.006450 0.003097 0.002453 0.002408 0.001681
Nucleolus 0.016049 0.010630 0.012761 0.013596 0.021116 0.008545 0.007430 0.002884 0.020609 0.009531 0.012122 0.013245
Nucleus 0.120029 0.069642 0.528561* 0.020987 0.150326* 0.049828 0.407146* 0.023120 0.147290* 0.059197 0.514022* 0.021056
Peroxisomes 0.019599 0.038900 0.004579 0.013285 0.010707 0.058905 0.008714 0.020490 0.011598 0.049446 0.005074 0.013521
Vacuole 0.041389 0.037182 0.034178 0.015398 0.024697 0.025163 0.078843 0.140244 0.026369 0.030846 0.039526 0.019482
Vacuole Periphery 0.000855 0.002611 0.000722 0.001963 0.000900 0.001958 0.000326 0.000427 0.000896 0.002267 0.000674 0.001913

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.43 -6.70 -5.07 -2.69 0.44 -4.13 -7.03 -2.80 -0.76 0.36 -5.62 -15.48 -8.93 0.02 1.26
Bud Neck -0.17 0.10 -0.90 -1.56 -2.16 -2.30 2.56 0.69 1.37 -0.37 -2.22 -0.46 -2.99 -1.24 -2.71
Bud Site -2.76 -2.48 -9.48 -8.93 -9.16 -7.76 -2.75 -2.89 -2.12 -2.28 -10.21 -7.85 -15.92 -10.69 -9.76
Cell Periphery 0.65 1.89 2.32 3.56 1.24 -2.32 1.33 2.58 3.67 1.37 -1.82 3.15 4.49 5.01 1.55
Cytoplasm 2.21 10.16 13.00 26.55 10.83 10.96 29.11 6.62 3.13 -2.70 10.01 30.48 37.87 32.24 9.56
Cytoplasmic Foci 1.38 15.51 14.99 37.75 -5.66 -8.10 32.31 23.25 29.88 -1.53 -5.43 43.00 40.87 58.38 -5.05
Eisosomes 2.84 4.32 4.53 11.50 1.91 -4.25 -1.25 0.45 3.50 1.29 -1.98 5.55 6.87 11.89 3.95
Endoplasmic Reticulum -1.71 -18.66 -5.68 -5.30 15.28 2.08 -9.06 -3.89 -4.14 4.23 0.90 -20.78 -6.00 -7.01 16.59
Endosome -2.30 3.11 3.08 12.65 -0.13 -9.20 2.29 -0.89 1.18 -1.34 -10.63 8.58 7.74 17.91 -0.06
Golgi -7.14 4.83 1.78 8.59 -3.40 -11.46 8.34 2.61 11.44 -0.60 -13.64 12.62 2.89 14.02 -3.34
Lipid Particles -2.78 4.80 5.33 9.59 3.02 -0.61 6.70 2.28 2.57 -0.85 -0.57 11.62 12.46 14.51 3.66
Mitochondria -4.14 1.03 0.22 5.98 -1.95 -6.99 6.30 5.08 9.48 -0.79 -8.42 4.30 1.42 9.14 -2.14
Mitotic Spindle -5.74 -13.18 -47.57* -48.95* -35.14* -7.83 -9.24 -5.69 -4.80 -1.11 -10.53 -19.14 -60.02 -52.90* -34.15*
None -0.59 2.33 4.09 9.48 12.99 10.73 11.38 14.29 6.96 2.69 10.52 13.25 15.01 12.48 12.43
Nuclear Periphery -2.62 -24.87 -8.55 -7.65 12.22 1.27 -5.79 -2.53 -3.67 2.81 1.45 -12.70 -3.16 -6.42 12.29
Nuclear Periphery Foci 0.00 -0.21 0.79 1.83 2.35 2.86 -3.33 -1.76 -2.14 0.49 1.79 -1.71 1.98 0.50 3.39
Nucleolus 1.76 0.39 1.65 -0.32 3.16 8.81 2.29 5.99 0.84 2.48 8.81 3.62 7.06 -1.16 3.42
Nucleus 3.35 -26.70 5.01 4.29 43.97 16.13 -8.88 4.25 -0.89 9.99 15.51 -35.33 15.72 2.46 44.80
Peroxisomes -3.67 5.12 3.58 13.43 -6.28 -16.84 2.79 0.15 8.37 -0.90 -18.77 11.02 4.51 21.64 -5.88
Vacuole 0.01 -2.31 -0.65 -1.02 2.17 -1.17 -6.71 -4.43 -4.37 -1.78 -3.53 -10.31 -5.68 -4.04 2.79
Vacuole Periphery -3.22 0.29 -1.39 2.24 -2.58 -3.69 3.21 3.90 5.42 0.88 -5.45 1.35 -2.02 2.22 -2.66
Standard name
Human Ortholog
Description Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ase1

Ase1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ase1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available