Standard name
Human Ortholog
Description Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.84 0.8 0.73 0.8 0.54 0.43 0.43 0.32 0.36 0.25 0.35 0.86 0.81 0.62 0.12 0.14 0.15 0.34 0.45 0.19 0.69 0.61 0.52
Endoplasmic Reticulum 0.08 0.13 0.07 0.05 0 0 0 0 0.06 0 0 0 0.09 0.07 0.12 0.07 0.06 0 0.24 0.18 0.28 0.08 0.08 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.17 0.57 0.53 0.53 0 0.05 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0.06 0.27 0.21 0.55 0.67 0.64 0.72 0.76 0.87 0.75 0 0 0 0.12 0.12 0.11 0.21 0.13 0.3 0.1 0.13 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.16 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0 0.05 0 0.16 0.13 0.16 0.08 0.08 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 8 2 1 0 0 0 1 1 0 0 5 3 9 4 7 10 8 8 18 3 7 10
Bud 1 0 1 4 8 15 30 23 13 26 9 15 0 0 0 1 2 3 0 1 1 1 7 10
Bud Neck 1 0 1 0 0 2 0 0 1 0 0 0 1 3 7 0 0 0 5 5 4 2 5 11
Bud Site 0 0 0 0 0 0 2 7 0 12 9 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 2 4 1 4 0 2 1 2 1 0 0 1 1 0 0 2 1 6 6 6 8
Cytoplasm 167 147 238 143 250 194 230 241 137 233 92 152 245 266 231 27 32 25 88 88 71 205 255 235
Endoplasmic Reticulum 16 22 21 9 4 12 6 2 24 0 0 3 25 23 44 16 14 5 60 36 106 24 34 60
Endosome 3 1 9 4 9 2 4 11 2 2 0 1 6 19 65 124 122 89 8 10 18 1 13 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 14 5 0 1 5 0 1 3
Mitochondria 20 9 19 53 66 197 361 364 311 488 320 332 3 0 1 26 29 19 53 25 112 28 53 47
Nucleus 0 1 1 0 2 1 5 9 2 3 3 8 0 4 2 4 4 2 1 0 0 0 1 0
Nuclear Periphery 1 0 0 0 0 2 1 1 5 0 0 2 1 0 0 2 3 3 1 0 1 0 0 1
Nucleolus 0 0 0 0 1 0 0 2 0 0 1 1 0 0 0 2 3 7 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 2 0 1 1 0 0 1 4 7 4 1 0 0 1 0 0 0
SpindlePole 1 0 7 2 0 1 3 14 7 5 3 9 4 13 29 35 38 25 1 0 5 2 3 7
Vac/Vac Membrane 3 4 7 3 12 12 22 26 15 28 18 28 3 15 11 35 30 27 19 15 14 11 22 31
Unique Cell Count 201 174 298 195 311 360 541 566 434 640 368 440 284 329 373 216 232 169 257 199 374 296 421 450
Labelled Cell Count 215 185 316 222 357 439 668 702 520 800 458 557 293 347 406 290 302 221 257 199 374 296 421 450


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.8 4.4 4.1 4.7 3.8 3.7 3.6 3.3 3.3 2.8 2.9 5.8 6.1 6.0 7.9 8.0 7.6 5.7 6.0 6.5
Std Deviation (1e-4) 0.6 0.9 0.8 0.9 1.8 1.2 1.4 1.5 1.0 1.2 0.7 1.0 1.0 1.1 1.1 1.5 1.8 1.4 1.1 1.5 1.5
Intensity Change (Log2) – – – -0.09 0.11 -0.19 -0.25 -0.27 -0.41 -0.43 -0.67 -0.59 0.4 0.47 0.45 0.84 0.86 0.8 0.37 0.45 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.7 4.7 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2132 4.374 4.0365 4.1922 3.7017 4.1388 1.8039 2.0219 1.8627 1.5081 1.3457 2.008 0.9009 1.0422 0.9192 1.0739 0.7854 1.016
Actin 0.0066 0.0022 0.0048 0.0093 0.0021 0.0021 0.0244 0.0014 0.0074 0.022 0.0078 0.0036 0.0064 0.0006 0.0053 0.0142 0.011 0.0014
Bud 0.0001 0.0001 0.0002 0.0005 0.0001 0.0001 0.0003 0.0003 0.0002 0.0005 0.0007 0.0006 0.0023 0.0008 0.0013 0.0001 0.0002 0.0006
Bud Neck 0.0011 0.0011 0.0012 0.0013 0.0013 0.0034 0.0046 0.0022 0.0071 0.0013 0.0018 0.0027 0.0054 0.0005 0.0007 0.0005 0.001 0.001
Bud Periphery 0.0002 0.0002 0.0003 0.0013 0.0002 0.0002 0.0004 0.0002 0.0003 0.0008 0.0013 0.0009 0.0027 0.0014 0.0005 0.0002 0.0003 0.0004
Bud Site 0.0047 0.0068 0.0018 0.0007 0.0005 0.0006 0.0024 0.0197 0.0045 0.0023 0.0011 0.0005 0.0049 0.0088 0.0068 0.0007 0.0005 0.0003
Cell Periphery 0.001 0.0018 0.0015 0.002 0.0031 0.0008 0.0009 0.0006 0.0008 0.0005 0.0003 0.0005 0.0023 0.0005 0.0005 0.0005 0.0004 0.0003
Cytoplasm 0.1177 0.1845 0.1396 0.2533 0.3455 0.182 0.0992 0.1679 0.1647 0.1156 0.1105 0.1893 0.1109 0.1005 0.1058 0.1942 0.0963 0.1493
Cytoplasmic Foci 0.1398 0.1194 0.1186 0.1379 0.1078 0.1246 0.1058 0.1102 0.1229 0.138 0.1183 0.1172 0.122 0.1505 0.1536 0.1285 0.2245 0.133
Eisosomes 0.0002 0.0003 0.0004 0.0001 0.0004 0.0002 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.1129 0.0939 0.0707 0.0721 0.1179 0.121 0.0827 0.0978 0.1017 0.069 0.1441 0.115 0.0665 0.0746 0.1101 0.0703 0.0845 0.0956
Endosome 0.1798 0.182 0.2045 0.1586 0.1168 0.1593 0.265 0.2885 0.2496 0.2478 0.2788 0.2135 0.239 0.3157 0.2685 0.3388 0.2329 0.2322
Golgi 0.0217 0.0396 0.0402 0.0321 0.0188 0.0215 0.0264 0.0195 0.0325 0.0464 0.0238 0.0242 0.0266 0.0332 0.0326 0.0313 0.0351 0.0297
Lipid Particles 0.3213 0.2387 0.261 0.1745 0.1233 0.241 0.2651 0.1899 0.1812 0.2592 0.2457 0.2473 0.2847 0.2311 0.1679 0.1421 0.2263 0.2422
Mitochondria 0.0019 0.008 0.0157 0.0035 0.0011 0.0055 0.0095 0.0024 0.0047 0.0099 0.002 0.0047 0.013 0.0026 0.0152 0.0039 0.0044 0.0066
None 0.051 0.0828 0.0916 0.0767 0.127 0.1029 0.035 0.044 0.0586 0.0175 0.0089 0.0202 0.0511 0.0248 0.0675 0.0187 0.0406 0.0524
Nuclear Periphery 0.0092 0.007 0.0046 0.0052 0.0041 0.0072 0.0251 0.0125 0.0125 0.0132 0.0237 0.0161 0.0077 0.0107 0.0116 0.0153 0.0062 0.0122
Nucleolus 0.0004 0.0005 0.0005 0.0003 0.0003 0.0003 0.0011 0.0032 0.0008 0.0004 0.0004 0.0008 0.0027 0.0012 0.0008 0.0004 0.0002 0.0004
Nucleus 0.0011 0.0014 0.0014 0.0018 0.0019 0.0018 0.0061 0.0027 0.0068 0.0022 0.0042 0.0045 0.0096 0.002 0.0039 0.0045 0.0014 0.0037
Peroxisomes 0.0121 0.0124 0.0179 0.0422 0.0176 0.009 0.0102 0.0042 0.0191 0.0261 0.0042 0.0087 0.0109 0.0157 0.0238 0.0099 0.0221 0.0115
Punctate Nuclear 0.0038 0.0049 0.0049 0.007 0.0019 0.0037 0.0123 0.0074 0.0075 0.0136 0.0061 0.0076 0.0136 0.0057 0.0088 0.0122 0.0042 0.0112
Vacuole 0.0121 0.0108 0.017 0.0171 0.0076 0.0112 0.0205 0.023 0.0149 0.0111 0.014 0.0195 0.0157 0.0174 0.0122 0.0119 0.0065 0.0141
Vacuole Periphery 0.0012 0.0016 0.0017 0.0025 0.0009 0.0015 0.0028 0.0023 0.002 0.0024 0.0021 0.0026 0.0019 0.0016 0.0024 0.0018 0.0012 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3105 16.7677 12.9833 17.108 20.0899 3.2603 21.0079 17.4127 20.5159 18.6003
Translational Efficiency 0.5571 0.9319 0.6644 0.6008 0.3686 3.7287 1.0598 0.8828 0.6449 0.4629

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2172 101 2046 1300 2078 2251 198 425 4250 2352 2244 1725

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 679.59 826.46 835.13 853.62 691.02 763.94 937.34 920.71 685.18 766.62 844.15 870.15
Standard Deviation 73.96 98.18 95.52 126.00 78.24 93.82 113.01 130.25 76.30 94.86 101.42 130.30
Intensity Change Log 2 — 0.282280 0.297336 0.328929 — 0.144732 0.439845 0.414019 — 0.214572 0.370943 0.372456

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000368 0.002852 0.000412 0.004536 0.000497 0.000833 0.001131 0.016273 0.000431 0.000920 0.000475 0.007428
Bud Neck 0.021288 0.020477 0.010838 0.014259 0.012054 0.026297 0.003111 0.010672 0.016773 0.026047 0.010156 0.013375
Bud Site 0.006364 0.008267 0.012177 0.037058 0.002889 0.011034 0.003394 0.047138 0.004665 0.010915 0.011402 0.039541
Cell Periphery 0.000249 0.000464 0.000267 0.000266 0.000245 0.000412 0.000414 0.000285 0.000247 0.000414 0.000280 0.000271
Cytoplasm 0.372868* 0.121697* 0.417666* 0.231792* 0.353590* 0.196108* 0.256894* 0.215908* 0.363442* 0.192912* 0.403480* 0.227878*
Cytoplasmic Foci 0.295222* 0.337000* 0.036708 0.135902 0.273630* 0.250693* 0.130327 0.153845 0.284664* 0.254399* 0.044968 0.140323
Eisosomes 0.000593 0.000824 0.000077 0.000167 0.000632 0.000454 0.000338 0.000363 0.000612 0.000470 0.000100 0.000215
Endoplasmic Reticulum 0.008372 0.037940 0.007303 0.006873 0.018723 0.020205 0.006235 0.006244 0.013433 0.020967 0.007208 0.006718
Endosome 0.018272 0.110528 0.005640 0.087690 0.023986 0.090087 0.025322 0.063881 0.021066 0.090965 0.007376 0.081824
Golgi 0.008395 0.056085 0.000287 0.014939 0.011510 0.027815 0.001243 0.011141 0.009918 0.029029 0.000372 0.014004
Lipid Particles 0.049898 0.143077* 0.007012 0.039138 0.093905 0.139882* 0.031915 0.042197 0.071415 0.140019* 0.009209 0.039892
Mitochondria 0.007622 0.008392 0.001282 0.006109 0.010348 0.023256 0.000552 0.006324 0.008955 0.022618 0.001218 0.006162
Mitotic Spindle 0.002147 0.010243 0.005072 0.072826 0.000665 0.005261 0.006459 0.079631 0.001422 0.005474 0.005194 0.074503
None 0.002127 0.000360 0.003008 0.002118 0.002752 0.002672 0.003289 0.001212 0.002433 0.002573 0.003033 0.001895
Nuclear Periphery 0.001107 0.001296 0.004551 0.003312 0.002382 0.004207 0.004730 0.001973 0.001730 0.004082 0.004567 0.002982
Nuclear Periphery Foci 0.001076 0.014725 0.005402 0.011426 0.002354 0.004087 0.013990 0.016994 0.001701 0.004544 0.006160 0.012798
Nucleolus 0.001253 0.001227 0.000998 0.002841 0.001621 0.004207 0.003718 0.000995 0.001433 0.004079 0.001238 0.002387
Nucleus 0.139722* 0.018185 0.415978* 0.063995 0.132010* 0.064353 0.354786* 0.046150 0.135951* 0.062370 0.410579* 0.059598
Peroxisomes 0.009177 0.028914 0.001605 0.026064 0.009553 0.017383 0.013921 0.047132 0.009361 0.017878 0.002692 0.031255
Vacuole 0.052558 0.070182 0.063285 0.233703* 0.045127 0.105510 0.137736 0.230180* 0.048925 0.103993 0.069854 0.232835*
Vacuole Periphery 0.001319 0.007267 0.000433 0.004984 0.001529 0.005244 0.000495 0.001462 0.001422 0.005331 0.000439 0.004117

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.47 -0.63 -5.69 -0.21 -5.53 -1.45 -1.28 -4.04 -3.90 -3.68 -2.40 -0.69 -6.26 -5.46 -6.13
Bud Neck 0.23 5.41 4.75 1.32 -0.82 -8.35 8.49 0.06 6.65 -5.07 -5.57 4.29 3.23 7.57 -1.24
Bud Site -1.03 -3.60 -7.69 -6.09 -5.02 -6.91 -1.32 -6.30 -4.65 -5.94 -5.32 -4.99 -10.37 -7.19 -6.59
Cell Periphery -1.42 -0.77 1.93 1.71 2.63 -2.02 -4.87 0.41 2.19 5.35 -2.16 -1.64 1.79 2.61 3.40
Cytoplasm 15.98 -2.63 14.42 -7.38 15.84 22.39 6.45 9.58 -1.66 0.85 29.74 -2.44 17.01 -5.76 16.57
Cytoplasmic Foci -2.09 53.03 31.31 11.75 -15.94 3.68 14.07 16.20 13.99 -0.55 5.77 63.79 36.00 26.45 -16.72
Eisosomes -1.30 14.08 12.22 4.13 -5.37 2.00 3.17 3.69 3.03 0.96 2.66 11.25 9.36 8.75 -4.14
Endoplasmic Reticulum -2.75 0.41 2.54 3.00 2.68 -0.67 7.29 8.24 9.02 1.18 -4.43 5.70 8.18 9.89 2.97
Endosome -6.77 12.39 -11.68 3.57 -15.48 -22.06 -0.21 -4.71 9.18 -3.61 -24.91 15.03 -12.09 8.97 -16.99
Golgi -4.41 14.99 -0.07 4.37 -6.07 -9.27 12.88 3.01 9.50 -3.09 -11.50 20.80 1.68 10.86 -6.91
Lipid Particles -5.07 19.19 7.07 6.27 -8.35 -8.48 12.49 12.04 18.81 0.19 -15.14 29.05 15.17 24.76 -8.62
Mitochondria -0.42 11.07 3.72 2.02 -3.23 -7.96 10.50 2.66 7.27 -1.31 -9.90 13.90 5.07 12.26 -3.12
Mitotic Spindle -1.38 -2.81 -12.19 -5.57 -11.34 -5.06 -1.74 -6.95 -6.39 -5.85 -4.37 -4.00 -14.16 -12.97 -12.87
None 6.13 -1.92 0.43 -3.38 1.78 0.33 -0.15 2.01 1.57 1.36 -0.17 -0.91 1.58 1.30 2.25
Nuclear Periphery -0.60 -12.81 -6.38 -5.03 2.19 -3.39 -1.89 -0.08 3.12 1.83 -5.38 -9.93 -4.82 0.97 3.33
Nuclear Periphery Foci -2.89 -7.49 -9.30 0.88 -2.94 -2.97 -4.19 -4.33 -3.73 0.30 -5.13 -7.80 -9.40 -6.09 -3.10
Nucleolus -0.12 0.08 -2.63 -2.06 -2.65 -5.17 -2.30 0.08 4.43 2.24 -6.13 -0.18 -2.32 2.23 -2.24
Nucleus 25.79 -38.94 10.94 -12.58 44.35 17.13 -9.82 10.30 0.04 12.69 22.97 -42.16 14.16 -4.24 47.59
Peroxisomes -3.74 12.29 -4.87 2.08 -9.05 -7.48 -0.88 -4.95 -3.22 -3.23 -8.94 11.14 -6.86 -1.89 -10.39
Vacuole -1.95 -7.93 -32.33 -20.38 -27.54 -17.04 -9.32 -18.64 -14.36 -7.09 -16.68 -12.13 -37.77 -27.77 -29.66
Vacuole Periphery -1.60 8.48 -3.36 1.13 -5.26 -12.85 4.41 1.83 10.87 -1.03 -13.15 9.75 -2.72 6.09 -5.41
Standard name
Human Ortholog
Description Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (22%), cytoplasm (12%), mixed (47%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.0001)
Subcompartmental Group N/A

Lpl1

Lpl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lpl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available