Standard name | |
---|---|
Human Ortholog | |
Description | Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 5 | 4 | – | 7 | 11 | 6 | 7 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 10 | 10 |
Bud Neck | 0 | 0 | 0 | 7 | 0 | 7 | 9 | – | 4 | 12 | 5 | 9 | 0 | 10 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 10 | 3 | – | 13 | 17 | 15 | 21 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 8 | 14 | 13 | 11 | 35 | – | 16 | 22 | 4 | 12 | 24 | 44 | 61 | 6 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 6 | 4 | 3 | 1 | 23 | 1 | 46 | – | 4 | 15 | 12 | 8 | 10 | 5 | 47 | 0 | 5 | 1 | 6 | 4 | 4 | 4 | 16 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 0 |
Mitochondria | 6 | 7 | 1 | 2 | 6 | 85 | 20 | – | 80 | 43 | 53 | 51 | 2 | 6 | 0 | 9 | 9 | 12 | 0 | 1 | 0 | 3 | 3 | 2 |
Nucleus | 71 | 40 | 39 | 29 | 57 | 47 | 50 | – | 49 | 44 | 45 | 42 | 34 | 58 | 48 | 36 | 47 | 33 | 50 | 37 | 20 | 26 | 51 | 64 |
Nuclear Periphery | 3 | 0 | 2 | 0 | 0 | 1 | 3 | – | 2 | 1 | 3 | 1 | 2 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 182 | 76 | 117 | 195 | 194 | 192 | 253 | – | 208 | 290 | 197 | 196 | 195 | 312 | 288 | 60 | 93 | 74 | 173 | 62 | 118 | 155 | 318 | 306 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 3 | – | 1 | 3 | 0 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 6 | 2 | 36 | 119 | 86 | 70 | 192 | – | 85 | 172 | 87 | 123 | 45 | 95 | 79 | 6 | 14 | 5 | 9 | 4 | 11 | 22 | 63 | 43 |
Vac/Vac Membrane | 0 | 3 | 0 | 1 | 3 | 1 | 2 | – | 2 | 2 | 4 | 4 | 3 | 4 | 3 | 0 | 1 | 1 | 3 | 1 | 4 | 14 | 15 | 10 |
Unique Cell Count | 235 | 100 | 155 | 226 | 277 | 285 | 398 | 318 | 406 | 284 | 304 | 241 | 396 | 399 | 95 | 133 | 100 | 252 | 118 | 168 | 234 | 506 | 462 | |
Labelled Cell Count | 277 | 137 | 206 | 369 | 385 | 430 | 621 | 471 | 633 | 431 | 475 | 317 | 537 | 535 | 122 | 182 | 133 | 252 | 118 | 168 | 234 | 506 | 462 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 8.3 | 8.6 | 8.2 | 8.1 | 5.2 | 6.8 | – | 5.3 | 6.0 | 5.7 | 5.8 | 9.7 | 8.0 | 9.3 | 10.3 | 10.4 | 10.6 | 8.7 | 9.0 | 9.1 |
Std Deviation (1e-4) | 2.1 | 1.8 | 2.4 | 2.2 | 2.0 | 1.9 | 2.1 | – | 1.4 | 1.6 | 1.3 | 1.8 | 3.1 | 2.7 | 3.1 | 3.6 | 2.7 | 2.8 | 2.1 | 2.5 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.71 | -0.33 | – | -0.68 | -0.51 | -0.59 | -0.57 | 0.18 | -0.1 | 0.12 | 0.27 | 0.28 | 0.3 | 0.02 | 0.08 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.9 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.2 | -0.6 | 1.4 | – | -0.1 | 0.1 | 0 | -0.6 | 1.7 | 2.1 | 3.2 | 0.4 | -0.3 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 0 | 3.6 | – | 0 | 0 | 1.3 | 0 | 1.2 | 0 | 3.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.4 | 2.4 | – | 6.6 | 3.9 | 5.5 | 5.2 | 0 | 0 | 0 | 0 | 0 | 4.0 |
Nucleus | -3.1 | -1.1 | -2.2 | -3.6 | – | -2.6 | -4.3 | -2.4 | -3.0 | -2.8 | -2.9 | -3.8 | 2.1 | 1.9 | 1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.7 | -1.2 | -1.8 | -2.7 | – | -2.2 | -1.0 | -1.4 | -2.4 | 1.3 | 0.8 | -0.8 | -2.1 | -1.1 | -0.3 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5.7 | 1.7 | 0.3 | 5.4 | – | 0.8 | 4.2 | 1.7 | 3.7 | -1.1 | 0.2 | -0.9 | -3.5 | -2.8 | -3.9 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9184 | 5.542 | 5.1092 | 4.0913 | 4.2327 | 5.0659 | 6.7522 | 7.5317 | 6.5906 | 6.0122 | 6.7012 | 6.5147 | 9.0112 | 10.1836 | 8.6571 | 9.1122 | 7.4927 | 8.7889 |
Actin | 0.0399 | 0.001 | 0.003 | 0.0015 | 0.0156 | 0.0125 | 0.0435 | 0 | 0.0051 | 0.0398 | 0.0022 | 0.0057 | 0.0048 | 0 | 0.016 | 0.0099 | 0.0012 | 0 |
Bud | 0.0007 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0007 | 0.0011 | 0.0005 | 0.0012 | 0.0003 | 0.0005 | 0.0011 | 0.0038 | 0.0003 | 0.0003 |
Bud Periphery | 0.0009 | 0.0003 | 0.0013 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0011 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 |
Bud Site | 0.0058 | 0.0024 | 0.0026 | 0.0003 | 0.002 | 0.0002 | 0.0012 | 0.001 | 0.0008 | 0.0041 | 0.0008 | 0.0007 | 0.0005 | 0.0004 | 0.0004 | 0.0024 | 0.0003 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasm | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0048 | 0 | 0.0026 | 0.0001 | 0.0039 | 0.0062 | 0.0047 | 0 | 0.0002 | 0.0005 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0165 | 0.0015 | 0.0026 | 0.0139 | 0.0313 | 0.0015 | 0.0122 | 0 | 0.0038 | 0.0002 | 0.0083 | 0.0052 | 0.0038 | 0.0002 | 0.001 | 0.0276 | 0.0175 | 0.0001 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0024 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0008 | 0.003 | 0 | 0.0027 | 0.0004 | 0.0025 | 0.0042 | 0.0021 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Endosome | 0.0243 | 0.0047 | 0.0084 | 0.0049 | 0.0302 | 0.0077 | 0.02 | 0 | 0.0029 | 0.0003 | 0.0141 | 0.0085 | 0.013 | 0.0001 | 0.0028 | 0.0024 | 0.0014 | 0 |
Golgi | 0.0284 | 0.0038 | 0.0014 | 0.009 | 0.0657 | 0.0019 | 0.0073 | 0 | 0.0018 | 0.0004 | 0.0022 | 0.0046 | 0.0011 | 0 | 0.0019 | 0.0025 | 0.0011 | 0 |
Lipid Particles | 0.0218 | 0.0007 | 0.0067 | 0.0125 | 0.0244 | 0.0037 | 0.0158 | 0.0001 | 0.0077 | 0.0004 | 0.0412 | 0.0084 | 0.0029 | 0.0005 | 0.0014 | 0.0046 | 0.0049 | 0.0004 |
Mitochondria | 0.0072 | 0.0052 | 0.0159 | 0.0013 | 0.0308 | 0.0023 | 0.005 | 0 | 0.0014 | 0.0043 | 0.0039 | 0.0015 | 0.0009 | 0.0002 | 0.0003 | 0.0088 | 0.0001 | 0.0001 |
None | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0062 | 0 | 0.0023 | 0.0004 | 0.0021 | 0.0011 | 0.007 | 0 | 0.0004 | 0.0009 | 0.0002 | 0 |
Nuclear Periphery | 0.003 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0071 | 0.0227 | 0 | 0.0018 | 0.0003 | 0.0167 | 0.0066 | 0.0132 | 0 | 0.0008 | 0.0002 | 0.0007 | 0 |
Nucleolus | 0.7556 | 0.8263 | 0.8685 | 0.8131 | 0.6664 | 0.8579 | 0.7486 | 0.8453 | 0.8386 | 0.8336 | 0.7349 | 0.8263 | 0.8327 | 0.8506 | 0.8793 | 0.8026 | 0.7686 | 0.9113 |
Nucleus | 0.0785 | 0.1507 | 0.081 | 0.1292 | 0.1055 | 0.0912 | 0.084 | 0.1523 | 0.1188 | 0.095 | 0.1141 | 0.1066 | 0.0966 | 0.1443 | 0.0906 | 0.1038 | 0.1174 | 0.0825 |
Peroxisomes | 0.0083 | 0.0003 | 0.0028 | 0.0116 | 0.0217 | 0.0006 | 0.0055 | 0 | 0.0027 | 0.0003 | 0.0026 | 0.0006 | 0.0006 | 0 | 0.0005 | 0.0211 | 0.0086 | 0 |
Punctate Nuclear | 0.0022 | 0.001 | 0.0019 | 0.0011 | 0.0009 | 0.0023 | 0.0156 | 0.0004 | 0.0046 | 0.0137 | 0.0461 | 0.0102 | 0.0138 | 0.0009 | 0.0015 | 0.0049 | 0.0769 | 0.0049 |
Vacuole | 0.0011 | 0.0006 | 0.0007 | 0.0005 | 0.0011 | 0.0039 | 0.0015 | 0.0001 | 0.0008 | 0.001 | 0.0014 | 0.0011 | 0.0008 | 0.0016 | 0.0006 | 0.0018 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0004 | 0.0002 | 0.0012 | 0.0044 | 0.0016 | 0 | 0.0003 | 0.0002 | 0.0013 | 0.0008 | 0.0008 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.0318 | 39.7609 | 73.7608 | 46.5686 | 58.7771 | 42.7439 | 42.9081 | 38.6135 | 38.6776 | 54.2969 |
Translational Efficiency | 2.2114 | 2.8216 | 1.207 | 1.6367 | 1.6923 | 1.7424 | 1.5433 | 1.2873 | 1.1553 | 1.2611 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
221 | 753 | 1512 | 1300 | 2261 | 2252 | 1985 | 41 | 2482 | 3005 | 3497 | 1341 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 900.25 | 1134.10 | 1030.32 | 1124.99 | 915.90 | 1030.90 | 1078.51 | 975.12 | 914.51 | 1056.76 | 1057.67 | 1120.41 |
Standard Deviation | 138.97 | 220.19 | 169.92 | 186.68 | 152.63 | 180.76 | 155.59 | 191.72 | 151.53 | 196.56 | 163.69 | 188.61 |
Intensity Change Log 2 | — | 0.333150 | 0.194695 | 0.321515 | — | 0.170642 | 0.235778 | 0.090390 | — | 0.253484 | 0.215559 | 0.209582 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000293 | 0.000703 | 0.000354 | 0.000338 | 0.000162 | 0.000336 | 0.000227 | 0.000562 | 0.000173 | 0.000428 | 0.000282 | 0.000345 |
Bud Neck | 0.002317 | 0.006225 | 0.007289 | 0.006153 | 0.002144 | 0.006677 | 0.004908 | 0.006084 | 0.002159 | 0.006564 | 0.005937 | 0.006151 |
Bud Site | 0.000833 | 0.001580 | 0.000840 | 0.000393 | 0.000314 | 0.001434 | 0.000636 | 0.000572 | 0.000360 | 0.001470 | 0.000724 | 0.000399 |
Cell Periphery | 0.000042 | 0.000076 | 0.000041 | 0.000034 | 0.000039 | 0.000107 | 0.000027 | 0.000038 | 0.000039 | 0.000099 | 0.000033 | 0.000034 |
Cytoplasm | 0.004938 | 0.000245 | 0.000394 | 0.000064 | 0.000774 | 0.001816 | 0.000607 | 0.000203 | 0.001145 | 0.001422 | 0.000515 | 0.000069 |
Cytoplasmic Foci | 0.001187 | 0.001672 | 0.000594 | 0.000075 | 0.000473 | 0.001078 | 0.000364 | 0.000186 | 0.000537 | 0.001227 | 0.000464 | 0.000078 |
Eisosomes | 0.000086 | 0.000160 | 0.000062 | 0.000115 | 0.000089 | 0.000126 | 0.000159 | 0.000060 | 0.000088 | 0.000134 | 0.000117 | 0.000114 |
Endoplasmic Reticulum | 0.001100 | 0.000843 | 0.000803 | 0.000554 | 0.000774 | 0.001284 | 0.000488 | 0.001417 | 0.000803 | 0.001174 | 0.000624 | 0.000580 |
Endosome | 0.000420 | 0.001197 | 0.000603 | 0.000153 | 0.000217 | 0.001250 | 0.000230 | 0.000150 | 0.000235 | 0.001237 | 0.000391 | 0.000153 |
Golgi | 0.000494 | 0.000524 | 0.000443 | 0.000124 | 0.000198 | 0.000424 | 0.000171 | 0.000179 | 0.000224 | 0.000449 | 0.000288 | 0.000125 |
Lipid Particles | 0.000579 | 0.000173 | 0.000053 | 0.000029 | 0.000122 | 0.000394 | 0.000019 | 0.000029 | 0.000162 | 0.000339 | 0.000034 | 0.000029 |
Mitochondria | 0.000646 | 0.001837 | 0.001029 | 0.001053 | 0.000472 | 0.000986 | 0.000628 | 0.001165 | 0.000487 | 0.001199 | 0.000802 | 0.001056 |
Mitotic Spindle | 0.013082 | 0.004083 | 0.009773 | 0.001405 | 0.002878 | 0.002796 | 0.001219 | 0.002094 | 0.003787 | 0.003119 | 0.004917 | 0.001426 |
None | 0.000688 | 0.000609 | 0.001135 | 0.000400 | 0.000754 | 0.002106 | 0.000519 | 0.001373 | 0.000748 | 0.001731 | 0.000786 | 0.000430 |
Nuclear Periphery | 0.000373 | 0.000221 | 0.000326 | 0.000497 | 0.000127 | 0.000263 | 0.000114 | 0.000130 | 0.000149 | 0.000252 | 0.000205 | 0.000485 |
Nuclear Periphery Foci | 0.002840 | 0.001294 | 0.000538 | 0.000405 | 0.001519 | 0.000790 | 0.000241 | 0.000138 | 0.001637 | 0.000916 | 0.000369 | 0.000397 |
Nucleolus | 0.663244* | 0.622350* | 0.574453* | 0.512309* | 0.562556* | 0.492753* | 0.513158* | 0.474368* | 0.571522* | 0.525227* | 0.539660* | 0.511149* |
Nucleus | 0.288578* | 0.339638* | 0.394065* | 0.469712* | 0.421938* | 0.478439* | 0.471170* | 0.503289* | 0.410063* | 0.443658* | 0.437832* | 0.470739* |
Peroxisomes | 0.016972 | 0.013007 | 0.005054 | 0.001865 | 0.003937 | 0.004693 | 0.003600 | 0.007218 | 0.005098 | 0.006776 | 0.004229 | 0.002029 |
Vacuole | 0.000806 | 0.001524 | 0.001243 | 0.001696 | 0.000251 | 0.001333 | 0.000794 | 0.000313 | 0.000301 | 0.001381 | 0.000988 | 0.001654 |
Vacuole Periphery | 0.000482 | 0.002040 | 0.000910 | 0.002625 | 0.000264 | 0.000915 | 0.000721 | 0.000433 | 0.000283 | 0.001197 | 0.000803 | 0.002558 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.00 | -1.42 | -0.80 | 0.89 | 1.68 | -6.48 | -4.39 | -3.43 | -2.23 | -2.98 | -2.50 | -8.58 | -10.26 | 0.73 | -3.60 |
Bud Neck | -4.31 | -10.76 | -7.06 | -0.28 | 2.05 | -11.19 | -9.77 | -2.58 | -0.41 | -1.21 | -11.79 | -16.70 | -9.28 | -0.05 | -0.77 |
Bud Site | -1.02 | -0.10 | 1.21 | 2.10 | 5.09 | -5.30 | -3.29 | -1.48 | 1.11 | -0.64 | -5.13 | -4.64 | -0.81 | 4.95 | 4.54 |
Cell Periphery | -1.23 | 0.17 | 0.70 | 1.87 | 1.11 | -4.98 | 1.44 | -0.37 | 2.57 | -0.93 | -4.92 | 0.76 | 0.52 | 5.43 | -0.12 |
Cytoplasm | 1.27 | 1.22 | 1.33 | 2.33 | 3.19 | -1.28 | 0.36 | 1.00 | 2.29 | 0.67 | -0.25 | 1.16 | 2.21 | 3.09 | 2.31 |
Cytoplasmic Foci | -0.77 | 0.85 | 1.75 | 2.46 | 1.45 | -1.51 | -0.01 | 1.19 | 3.01 | 1.03 | -1.96 | 0.09 | 2.06 | 4.35 | 2.02 |
Eisosomes | -4.82 | 4.48 | -3.13 | 2.80 | -6.91 | -5.33 | -9.05 | 4.27 | 7.32 | 10.27 | -7.06 | -5.78 | -3.27 | 2.29 | 0.46 |
Endoplasmic Reticulum | 1.32 | 1.30 | 2.75 | 3.44 | 4.31 | -5.92 | 5.58 | -1.59 | -0.64 | -2.11 | -5.08 | 3.01 | 3.07 | 7.54 | 0.96 |
Endosome | -1.30 | -0.64 | 1.93 | 1.82 | 1.98 | -3.68 | -0.18 | 0.20 | 3.65 | 0.31 | -3.92 | -1.34 | 1.30 | 4.24 | 2.03 |
Golgi | -0.07 | 0.41 | 6.16 | 3.05 | 1.98 | -3.42 | 0.35 | 0.08 | 3.17 | -0.25 | -3.68 | -0.88 | 3.88 | 5.45 | 2.21 |
Lipid Particles | 0.87 | 1.11 | 1.18 | 2.44 | 1.79 | -1.35 | 1.85 | 1.39 | 1.90 | -1.30 | -1.07 | 1.93 | 1.99 | 2.21 | 0.43 |
Mitochondria | -2.17 | -3.10 | -2.74 | 1.30 | -0.30 | -4.86 | -1.88 | -2.91 | -1.20 | -2.33 | -4.45 | -4.31 | -4.60 | 0.39 | -2.20 |
Mitotic Spindle | 2.43 | 0.76 | 3.19 | 2.06 | 5.15 | 0.08 | 2.10 | -0.80 | -0.80 | -0.96 | 0.89 | -1.49 | 2.37 | 1.82 | 3.68 |
None | 0.46 | -1.49 | 1.83 | 1.37 | 2.69 | -3.79 | 1.45 | -0.94 | 0.81 | -1.24 | -3.55 | -0.26 | 2.18 | 4.84 | 2.39 |
Nuclear Periphery | 1.41 | 0.23 | -0.93 | -2.04 | -1.23 | -2.70 | 0.30 | -0.89 | 0.01 | -0.94 | -2.63 | -2.37 | -2.83 | -1.86 | -2.26 |
Nuclear Periphery Foci | 2.46 | 4.06 | 4.25 | 2.44 | 0.71 | 2.07 | 3.61 | 3.03 | 2.09 | -0.31 | 2.20 | 3.89 | 3.57 | 2.90 | -0.27 |
Nucleolus | 2.43 | 5.77 | 9.41 | 9.69 | 5.80 | 9.47 | 6.36 | 2.68 | 0.93 | 1.42 | 6.95 | 5.10 | 7.01 | 1.83 | 3.27 |
Nucleus | -3.28 | -7.10 | -11.79 | -11.68 | -7.46 | -7.73 | -6.36 | -2.33 | -0.84 | -1.02 | -5.09 | -4.37 | -7.06 | -3.27 | -3.88 |
Peroxisomes | 2.12 | 6.81 | 8.77 | 12.72 | 10.84 | -2.00 | 1.45 | -1.45 | -1.07 | -1.62 | -4.05 | 2.99 | 11.94 | 13.32 | 11.97 |
Vacuole | -1.90 | -1.14 | -1.52 | -0.36 | -0.54 | -4.80 | -3.08 | -1.51 | 4.00 | 2.23 | -5.93 | -3.78 | -2.73 | -0.55 | -1.12 |
Vacuole Periphery | -1.60 | -1.21 | -3.04 | -0.50 | -2.25 | -4.64 | -4.14 | -3.41 | 3.34 | 2.56 | -3.48 | -3.40 | -3.38 | -1.88 | -2.53 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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