Standard name
Human Ortholog
Description Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.07 0.05 0.07 0.08 0.08 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.26 0.2 0.15 0.11 0.13 0.08 0.13 0 0 0.09 0.23 0.44 0.43 0.46 0.48 0.51 0.43 0.58 0.5 0.31 0.3 0.34
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0
Mitochondria 0.59 0.61 0.29 0.22 0.09 0.24 0.41 0.32 0.66 0.73 0.62 0.38 0.15 0.12 0.09 0.06 0.08 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.13 0.05 0.14 0.09 0.14 0.14 0.09 0.18 0.17 0.18 0.13 0.11 0.16 0.15 0.25 0.26 0 0 0 0 0 0
Peroxisomes 0.33 0.19 0.17 0.16 0.14 0.16 0.09 0.08 0 0 0 0.58 0.62 0.55 0.44 0.32 0.24 0.32 0.16 0.28 0.44 0.44 0.47
SpindlePole 0.27 0.17 0.55 0.52 0.74 0.58 0.49 0.54 0.27 0.24 0.29 0.29 0.39 0.48 0.47 0.48 0.58 0.1 0.1 0.12 0.12 0.18 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 4 0 2 1 2 0 2 1 0 0 0 0 0 1 0 1 2 0 1 3 8
Bud 6 6 3 2 7 3 12 21 12 13 7 14 3 2 0 1 1 1 1 0 0 3 0
Bud Neck 1 0 0 2 3 2 3 2 2 2 1 0 1 0 1 1 0 1 0 0 0 0 1
Bud Site 2 0 0 3 3 3 0 2 7 8 7 2 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 8 10 12 14 24 29 15 5 15 6 15 13 3 5 2 0 0 1 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 1 1 0 0 1 0 1 1
Endosome 33 43 25 20 26 27 24 46 2 13 26 86 193 209 72 66 56 78 109 95 40 110 146
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 13 9 3 3 7 16
Mitochondria 104 102 37 30 21 50 131 114 164 223 183 141 67 58 14 8 9 3 9 4 5 4 9
Nucleus 1 0 0 2 0 2 6 6 11 9 11 0 3 2 0 0 1 0 0 0 0 0 0
Nuclear Periphery 7 4 3 2 1 2 4 5 3 14 5 1 3 0 0 1 1 2 1 1 0 0 0
Nucleolus 32 21 7 19 21 29 44 33 44 53 52 47 46 76 23 34 28 1 1 0 1 0 0
Peroxisomes 58 32 22 22 34 33 29 28 9 7 8 218 269 268 69 44 26 59 29 53 57 163 204
SpindlePole 47 29 70 71 180 120 156 192 66 74 87 109 168 231 74 66 63 19 18 22 15 64 38
Vac/Vac Membrane 2 5 1 1 6 1 2 2 3 4 9 3 1 3 1 7 2 0 0 0 0 0 1
Unique Cell Count 176 167 128 137 244 207 316 355 247 307 296 375 436 485 158 138 109 185 188 192 130 368 434
Labelled Cell Count 296 243 180 184 316 287 437 480 341 427 411 627 770 863 259 236 191 185 188 192 130 368 434


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.0 6.1 5.7 6.0 4.6 4.8 5.0 4.1 3.9 4.5 6.1 6.8 6.8 9.9 9.5 9.4 6.3 6.4 6.6
Std Deviation (1e-4) 1.4 1.6 1.2 1.4 1.4 1.2 1.3 1.4 1.1 0.9 1.0 1.7 1.9 1.6 2.2 2.1 1.9 1.5 1.4 1.4
Intensity Change (Log2) -0.11 -0.02 -0.41 -0.36 -0.29 -0.59 -0.67 -0.46 0.0 0.16 0.14 0.69 0.62 0.61 0.03 0.05 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 1.6 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 -2.4 0 0 -1.8 0 0 0.8 5.1 4.9 4.6 4.9 5.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 -5.1 0 0 0.7 0 0 1.8 -3.5 -4.7 -4.4 -5.0 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 2.3 1.1 0 0 1.3 0 0 2.2 1.7 3.0 2.5 4.3 4.4
Peroxisomes -0.2 -0.8 0 0 -3.0 0 0 8.0 8.9 7.7 4.8 2.8 1.3
SpindlePole -0.5 3.7 0 0 -0.1 0 0 -5.2 -3.3 -1.4 -1.3 -1.1 0.5
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.5 0.2 0.5 0.7 -0.1 -0.5 0 -1.4 0 0 -1.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5138 3.2491 2.2394 1.6951 1.558 1.9738 0.9628 3.4165 2.7503 2.9044 2.726 2.6384 0.689 3.4079 2.8478 2.3508 1.7637 2.6186
Actin 0.0078 0.0002 0.0005 0.0001 0.0002 0.0067 0.0193 0.0002 0.002 0.0063 0.0323 0.0004 0.0172 0 0.0113 0.0002 0.0387 0.0001
Bud 0 0 0 0 0 0 0.0007 0 0.0001 0.0002 0.0002 0 0.0002 0 0 0 0.0003 0
Bud Neck 0.0014 0 0.0001 0.0001 0.0004 0.0002 0.0052 0.0001 0.0028 0.0015 0.0005 0.0002 0.0003 0.0002 0.0004 0.0001 0.0006 0.0001
Bud Periphery 0 0 0.0001 0 0 0.0001 0.0009 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0 0 0.0005 0
Bud Site 0.0001 0 0.0002 0 0.0001 0.0001 0.0067 0.0002 0.0008 0.0018 0.0004 0.0001 0.001 0.0002 0.0011 0.0001 0.001 0
Cell Periphery 0.0001 0 0.0001 0 0.0006 0 0.0002 0 0.0003 0.0001 0 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0053 0 0.0001 0 0.0001 0.0004 0.0019 0.0001 0.0004 0.0003 0.0004 0.0001 0.002 0.0006 0.0001 0.0001 0.0009 0
Cytoplasmic Foci 0.1574 0.1805 0.1678 0.2676 0.1618 0.1622 0.2547 0.2523 0.2491 0.1854 0.1679 0.2063 0.219 0.1993 0.1765 0.1592 0.1426 0.1323
Eisosomes 0.0005 0 0 0 0 0.0001 0.0002 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.0015 0 0.0001 0 0 0.0004 0.0011 0.0001 0.0001 0.0001 0.0007 0.0001 0.0004 0.0005 0 0.0001 0.0016 0
Endosome 0.0134 0.0054 0.0083 0.0021 0.0095 0.0108 0.029 0.0163 0.0153 0.0262 0.0144 0.0101 0.0194 0.0161 0.0118 0.0564 0.0327 0.0036
Golgi 0.0158 0.012 0.0128 0.0071 0.01 0.0088 0.0193 0.0145 0.0136 0.029 0.0274 0.0102 0.0144 0.014 0.0154 0.0312 0.02 0.0096
Lipid Particles 0.7218 0.7632 0.6952 0.6205 0.5972 0.7657 0.5247 0.6317 0.5815 0.6365 0.5162 0.6963 0.6362 0.7069 0.6869 0.6716 0.6748 0.7966
Mitochondria 0.0035 0.0008 0.008 0.0008 0.0008 0.0036 0.0063 0.0007 0.0032 0.0086 0.0112 0.0051 0.0079 0.0005 0.0011 0.0014 0.0106 0.0006
None 0.001 0 0.0001 0 0 0.0014 0.001 0.0001 0.0002 0.0002 0.0001 0 0.0034 0.0006 0 0.0002 0.0032 0
Nuclear Periphery 0.0048 0.0001 0.0003 0 0 0.0034 0.0021 0.0002 0.0006 0.0002 0.0006 0.0003 0.0016 0.0008 0.0003 0.0002 0.0109 0.0005
Nucleolus 0.0035 0.0003 0.0049 0.0006 0.0119 0.0063 0.0064 0.0037 0.0049 0.0014 0.0002 0.001 0.0033 0.0047 0.0004 0.0006 0.0023 0.0007
Nucleus 0.0036 0 0.0003 0 0.0006 0.0015 0.0013 0.0003 0.0002 0.0003 0.0001 0.0001 0.001 0.0002 0 0 0.0017 0
Peroxisomes 0.0424 0.0339 0.0897 0.0959 0.1957 0.0147 0.0797 0.066 0.1034 0.0956 0.2096 0.0446 0.0332 0.0371 0.0789 0.0658 0.0333 0.0421
Punctate Nuclear 0.0149 0.0031 0.0082 0.0048 0.0017 0.0124 0.0366 0.0127 0.0206 0.0045 0.0168 0.0219 0.0376 0.0173 0.0153 0.0113 0.0203 0.0135
Vacuole 0.0004 0.0001 0.0018 0 0.0086 0.0007 0.0016 0.0004 0.0004 0.0011 0.0002 0.0013 0.0009 0.0006 0.0001 0.0006 0.0016 0.0001
Vacuole Periphery 0.0007 0.0002 0.0015 0.0001 0.0005 0.0006 0.0012 0.0004 0.0003 0.0005 0.0004 0.0017 0.0005 0.0003 0.0002 0.0007 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5777 10.5213 10.0844 13.1734 16.5315 14.7039 14.6274 13.4625 11.7903 21.7703
Translational Efficiency 0.4737 0.8924 0.5776 0.4359 0.6524 0.7088 0.4952 0.5203 0.6547 0.5571

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
817 1452 1300 1693 1735 1340 200 855 2552 2792 1500 2548

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 757.65 875.02 1069.67 1072.22 765.32 851.65 1213.91 1058.29 762.86 863.80 1088.90 1067.55
Standard Deviation 96.52 143.97 153.77 189.69 122.82 133.75 157.00 197.63 115.12 139.65 161.82 192.50
Intensity Change Log 2 0.207784 0.497562 0.500998 0.154198 0.665526 0.467600 0.181105 0.584410 0.484311

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002100 0.003611 0.005338 0.042045 0.003562 0.003101 0.008237 0.076106 0.003094 0.003366 0.005725 0.053474
Bud Neck 0.000411 0.001487 0.032814 0.005069 0.000670 0.001455 0.007995 0.004351 0.000587 0.001472 0.029504 0.004828
Bud Site 0.000154 0.001189 0.026778 0.011921 0.001117 0.001747 0.002462 0.008569 0.000808 0.001457 0.023536 0.010796
Cell Periphery 0.000047 0.000096 0.000423 0.000167 0.000119 0.000088 0.000091 0.000150 0.000096 0.000092 0.000379 0.000161
Cytoplasm 0.000369 0.002782 0.095593 0.005698 0.004652 0.003129 0.000775 0.004390 0.003281 0.002949 0.082950 0.005259
Cytoplasmic Foci 0.058009 0.157961 0.061828 0.256226* 0.123484 0.152711 0.213656* 0.224268* 0.102523 0.155441 0.082071 0.245502*
Eisosomes 0.000111 0.000135 0.000065 0.000181 0.000068 0.000072 0.000183 0.000400 0.000082 0.000105 0.000080 0.000254
Endoplasmic Reticulum 0.000391 0.000645 0.013710 0.000667 0.000549 0.000448 0.000903 0.000910 0.000498 0.000550 0.012003 0.000748
Endosome 0.031033 0.071892 0.019293 0.150649 0.059787 0.124463 0.070701 0.099998 0.050581 0.097123 0.026148 0.133653
Golgi 0.042824 0.069403 0.001966 0.110450* 0.046189 0.062848 0.061960 0.173144* 0.045112 0.066257 0.009965 0.131487*
Lipid Particles 0.761909* 0.588170* 0.051429 0.142068* 0.653991* 0.532153* 0.448846* 0.201952* 0.688540* 0.561285* 0.104418* 0.162162*
Mitochondria 0.012800 0.005212 0.002371 0.028978 0.003220 0.007732 0.001901 0.032444 0.006287 0.006421 0.002308 0.030141
Mitotic Spindle 0.007102 0.017124 0.178100* 0.090792 0.015795 0.013903 0.097339 0.034416 0.013012 0.015578 0.167332* 0.071874
None 0.000179 0.000523 0.002870 0.000125 0.001061 0.000281 0.000050 0.000133 0.000779 0.000407 0.002494 0.000128
Nuclear Periphery 0.000694 0.000423 0.007967 0.002030 0.000261 0.000296 0.003947 0.000896 0.000399 0.000362 0.007431 0.001650
Nuclear Periphery Foci 0.013714 0.012644 0.050147 0.004220 0.014119 0.009975 0.015176 0.002555 0.013989 0.011363 0.045485 0.003661
Nucleolus 0.001424 0.000607 0.005716 0.000357 0.001307 0.000558 0.001123 0.000568 0.001344 0.000584 0.005104 0.000428
Nucleus 0.000543 0.000386 0.392410* 0.000679 0.000895 0.000301 0.000542 0.000389 0.000782 0.000345 0.340161* 0.000582
Peroxisomes 0.065054 0.063731 0.018031 0.128148* 0.067790 0.081280 0.054606 0.124920* 0.066914 0.072154 0.022908 0.127065*
Vacuole 0.000619 0.001569 0.032640 0.015314 0.001106 0.002206 0.008376 0.007536 0.000950 0.001875 0.029405 0.012704
Vacuole Periphery 0.000516 0.000409 0.000510 0.004215 0.000260 0.001252 0.001132 0.001904 0.000342 0.000814 0.000593 0.003440

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.31 -4.54 -14.01 -13.55 -12.32 0.70 -2.05 -11.92 -12.00 -10.50 -0.58 -4.22 -17.96 -17.92 -16.36
Bud Neck -3.89 -18.90 -13.77 -8.48 16.49 -2.20 -5.43 -7.75 -5.14 2.61 -3.89 -19.03 -15.17 -9.89 16.60
Bud Site -5.84 -15.45 -11.42 -10.32 8.75 -0.97 -2.49 -5.96 -5.21 -4.64 -1.86 -15.02 -12.19 -11.19 8.79
Cell Periphery -2.75 -15.44 -7.81 -3.15 10.30 0.72 0.70 -0.68 -3.07 -3.75 0.13 -8.98 -2.10 -4.45 10.31
Cytoplasm -3.47 -22.55 -8.28 -3.49 20.51 1.39 4.42 0.19 -1.23 -4.38 0.43 -20.77 -3.01 -3.67 20.07
Cytoplasmic Foci -18.63 0.75 -33.33 -14.25 -33.06* -5.10 -7.45 -13.31 -9.03 -0.78 -12.76 6.11 -29.06 -17.36 -31.39
Eisosomes -1.53 2.40 -4.64 -3.29 -11.24 -0.60 -4.82 -12.68 -12.53 -6.16 -3.08 -1.70 -15.10 -12.47 -14.00
Endoplasmic Reticulum -1.91 -17.34 -3.81 -0.20 17.05 1.21 -2.66 -2.73 -3.61 0.03 -0.55 -17.04 -3.59 -2.28 16.75
Endosome -9.11 4.37 -23.72 -14.82 -31.65* -11.18 -1.19 -7.58 3.58 -2.97 -12.56 9.96 -21.93 -8.23 -31.04*
Golgi -7.02 15.58 -15.25 -9.03 -31.39* -4.66 -2.08 -18.07 -15.09 -11.37 -8.18 17.86 -23.33 -16.82 -35.29*
Lipid Particles 13.07 68.95* 55.53* 44.07* -16.53 9.45 8.81 38.12* 25.22 10.48 13.71 70.65 67.23 50.14* -9.58
Mitochondria 2.94 3.96 -4.70 -9.58 -11.00 -3.09 1.59 -7.79 -6.30 -8.19 -0.11 3.67 -10.86 -11.11 -13.62
Mitotic Spindle -4.01 -18.30 -16.52 -14.00 6.64 0.88 -5.26 -4.43 -4.85 3.80 -1.34 -18.36 -15.78 -14.94 9.10
None -1.19 -13.96 0.79 1.43 15.04 1.59 2.13 1.97 1.33 -6.29 1.06 -4.56 2.01 1.82 14.88
Nuclear Periphery 0.63 -10.68 -2.35 -4.12 9.09 -0.63 -5.07 -3.10 -2.92 4.05 0.27 -13.71 -4.19 -4.81 10.55
Nuclear Periphery Foci 0.65 -8.24 3.75 4.83 12.92 2.20 -0.17 6.99 4.75 3.86 1.89 -9.28 7.46 6.51 13.41
Nucleolus 1.74 -6.45 2.38 1.56 8.52 2.53 0.54 2.14 0.04 0.91 3.14 -6.82 3.81 1.02 8.45
Nucleus 0.54 -37.60 -0.64 -1.00 37.54 1.62 0.84 1.20 -0.41 0.31 1.68 -35.11 0.16 -1.09 35.11
Peroxisomes 0.39 9.65 -8.37 -9.99 -21.11* -2.36 1.43 -6.95 -5.03 -6.17 -1.16 13.91 -11.49 -10.60 -23.36*
Vacuole -4.58 -17.68 -11.33 -10.69 10.11 -2.20 -5.28 -6.97 -5.36 0.54 -3.40 -17.71 -12.62 -11.61 11.25
Vacuole Periphery 0.37 -0.10 -6.69 -8.04 -7.74 -2.76 -3.51 -6.72 -1.54 -2.23 -2.37 -2.49 -9.17 -7.16 -8.56