Standard name
Human Ortholog
Description Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.97 0.98 0.96 0.95 0.84 0.77 0.77 0.68 0.6 0.59 0.56 0.99 0.99 0.99 0.97 0.96 0.98 0.88 0.92 0.81 0.96 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0.14 0.12 0.37 0.49 0.39 0.51 0.58 0.6 0.67 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 2 0 1 1
Bud 1 1 3 2 1 8 23 8 10 18 21 14 0 0 0 1 0 0 0 1 4 0 4 13
Bud Neck 0 0 0 1 1 1 3 3 2 4 0 2 1 1 2 0 0 0 0 0 1 0 1 4
Bud Site 0 0 0 0 0 1 2 0 4 3 7 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 2 1 3 2 2 0 1 3 1 0 1 2 17 15 8 0 0 2 0 1 1
Cytoplasm 156 235 283 240 307 393 482 390 272 276 221 247 600 579 711 433 409 284 148 223 235 250 284 294
Endoplasmic Reticulum 3 0 0 1 0 0 0 0 0 2 0 2 0 1 1 15 24 8 0 0 4 1 1 0
Endosome 0 0 0 1 1 1 0 2 1 2 0 0 3 1 1 0 3 0 2 4 2 0 0 1
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 0 0 1 0 0 1
Mitochondria 25 8 3 35 38 174 308 198 203 266 223 292 0 0 0 5 4 1 5 8 23 1 5 7
Nucleus 0 0 1 0 0 1 3 0 1 1 1 1 0 0 4 3 0 2 0 0 0 0 0 2
Nuclear Periphery 0 1 0 0 0 1 1 0 1 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 4 5 8 5 10 9 9 12 0 0 3 0 0 0 1 0 1 0 1 6
Vac/Vac Membrane 0 0 0 1 2 4 7 2 5 2 3 7 1 2 1 18 13 8 3 1 2 1 3 2
Unique Cell Count 169 242 289 250 323 470 623 509 398 458 373 438 604 584 719 447 425 291 169 244 292 261 311 342
Labelled Cell Count 186 246 294 283 355 592 840 611 509 586 489 583 605 585 727 492 474 313 169 244 292 261 311 342


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.2 4.0 3.7 3.9 3.1 3.0 3.0 2.8 2.7 2.5 2.5 5.3 5.3 5.2 7.5 7.1 6.7 5.5 5.8 6.0
Std Deviation (1e-4) 0.7 0.7 1.3 1.7 1.7 1.2 1.2 1.4 1.0 0.9 1.3 1.0 1.0 1.2 1.3 1.6 1.4 1.2 1.1 1.6 2.0
Intensity Change (Log2) – – – -0.1 -0.03 -0.37 -0.4 -0.39 -0.49 -0.56 -0.65 -0.64 0.43 0.41 0.39 0.93 0.84 0.76 0.47 0.54 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.5 3.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.651 1.3269 1.8475 0.8138 1.4496 1.745 0.6623 0.2881 0.4619 0.2207 0.4326 0.2914 0.1532 -0.4895 -0.442 0.483 -0.0175 -0.6114
Actin 0.0363 0.002 0.0266 0.0003 0.0438 0.0061 0.0416 0.0005 0.0266 0.0377 0.004 0.008 0.0516 0.0037 0.0215 0.0311 0.0512 0.0064
Bud 0.0014 0.0034 0.0004 0.0005 0.0002 0.008 0.0015 0.0015 0.0024 0.0011 0.0011 0.0004 0.0044 0.0063 0.0004 0.003 0.001 0.0025
Bud Neck 0.0068 0.0001 0.0008 0.0313 0.0023 0.0007 0.0062 0.0006 0.0012 0.0069 0.0003 0.0026 0.0123 0.0001 0.0006 0.0052 0.0004 0.0004
Bud Periphery 0.0034 0.0018 0.0006 0.0009 0.0003 0.0053 0.0017 0.0015 0.0022 0.0017 0.0013 0.0009 0.0116 0.0035 0.0004 0.0033 0.0012 0.0044
Bud Site 0.0093 0.0037 0.0016 0.0035 0.0005 0.0006 0.0103 0.0065 0.0087 0.0237 0.0003 0.0002 0.0181 0.0099 0.006 0.0117 0.0046 0.004
Cell Periphery 0.0009 0.0002 0.0001 0.0001 0 0.0001 0.0003 0.0002 0.0003 0.0003 0.0001 0.0001 0.0007 0.0003 0.0002 0.0006 0.0002 0.0001
Cytoplasm 0.0537 0.0609 0.088 0.0598 0.1328 0.1181 0.1577 0.2684 0.2915 0.2912 0.3412 0.3954 0.116 0.273 0.2105 0.2328 0.229 0.3329
Cytoplasmic Foci 0.0118 0.0057 0.007 0.0279 0.0188 0.0069 0.0542 0.009 0.027 0.0255 0.0134 0.0087 0.055 0.0047 0.026 0.0222 0.0109 0.0117
Eisosomes 0.0004 0.0002 0.0004 0.0001 0.0002 0.0001 0.0004 0.0003 0.0003 0.0003 0 0.0001 0.0005 0.0002 0.0002 0.0003 0.0002 0
Endoplasmic Reticulum 0.0117 0.0005 0.0008 0.0004 0.0012 0.0009 0.0086 0.0013 0.009 0.0027 0.0033 0.0035 0.0036 0.0031 0.0016 0.0056 0.0024 0.0008
Endosome 0.0163 0.0019 0.0023 0.003 0.024 0.0035 0.0327 0.001 0.0199 0.0221 0.0061 0.0132 0.0266 0.002 0.015 0.0824 0.0251 0.0031
Golgi 0.0042 0.0002 0.0018 0.0013 0.0118 0.0023 0.0091 0.0002 0.0116 0.0164 0.0006 0.0045 0.0154 0.0004 0.0054 0.0192 0.0048 0.0008
Lipid Particles 0.0043 0.0004 0.0026 0.0004 0.0139 0.0033 0.017 0.0006 0.0091 0.0081 0.0008 0.0022 0.0265 0.0008 0.0044 0.0161 0.0046 0.0001
Mitochondria 0.0023 0.0006 0.0017 0.0004 0.0016 0.0041 0.0078 0.0005 0.0036 0.0197 0.0011 0.0039 0.007 0.0002 0.0009 0.0072 0.0037 0.0005
None 0.7989 0.9089 0.8462 0.8556 0.7289 0.8206 0.6036 0.7025 0.563 0.5262 0.6169 0.5482 0.5735 0.6869 0.6884 0.4874 0.6411 0.621
Nuclear Periphery 0.0145 0.0006 0.0018 0.0002 0.0013 0.0026 0.0142 0.0005 0.0032 0.0018 0.0019 0.002 0.0132 0.0009 0.0015 0.0187 0.0044 0.0004
Nucleolus 0.001 0.0004 0.0015 0.0005 0.0002 0.0007 0.001 0.0004 0.0003 0.001 0.0001 0.0001 0.0029 0.0001 0.0002 0.0013 0.001 0.0003
Nucleus 0.0059 0.003 0.0026 0.0005 0.0009 0.0052 0.0043 0.0014 0.002 0.0012 0.0027 0.0016 0.0093 0.0011 0.0012 0.0071 0.0036 0.0013
Peroxisomes 0.0042 0.0005 0.0041 0.012 0.0117 0.005 0.0125 0.0006 0.0126 0.0066 0.0005 0.0009 0.03 0.0003 0.0073 0.0033 0.0023 0.0031
Punctate Nuclear 0.0056 0.0045 0.0084 0.0007 0.0051 0.0052 0.0097 0.0014 0.0032 0.0029 0.001 0.002 0.0143 0.0009 0.0031 0.0296 0.0044 0.0006
Vacuole 0.0053 0.0005 0.0004 0.0006 0.0004 0.0005 0.0037 0.0009 0.0018 0.0021 0.0025 0.0011 0.0058 0.0014 0.0046 0.0089 0.0034 0.005
Vacuole Periphery 0.0017 0.0001 0.0002 0.0001 0.0003 0.0003 0.0019 0.0001 0.0005 0.0008 0.0006 0.0005 0.0018 0.0001 0.0006 0.003 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.23 28.8117 11.3186 13.1153 33.1843 46.4234 31.0593 19.3726 22.3377 28.8273
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1699 705 2222 671 1371 1749 134 1273 3070 2454 2356 1944

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.21 743.25 782.73 751.96 665.71 725.53 897.95 811.47 678.16 730.62 789.28 790.93
Standard Deviation 76.50 98.76 94.49 96.50 77.28 89.37 91.34 109.30 77.66 92.51 98.02 108.80
Intensity Change Log 2 — 0.110999 0.185666 0.127807 — 0.124141 0.431741 0.285644 — 0.117476 0.311903 0.207572

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000114 0.000352 0.000086 0.000399 0.000068 0.000244 0.000247 0.000626 0.000094 0.000275 0.000095 0.000548
Bud Neck 0.016551 0.026729 0.007514 0.008121 0.006180 0.022316 0.004781 0.006940 0.011920 0.023584 0.007358 0.007348
Bud Site 0.003926 0.010636 0.009299 0.026335 0.001756 0.013717 0.004915 0.017081 0.002957 0.012832 0.009050 0.020275
Cell Periphery 0.000129 0.000182 0.000115 0.000259 0.000168 0.000299 0.000223 0.000241 0.000146 0.000265 0.000121 0.000247
Cytoplasm 0.506621* 0.417087* 0.478730* 0.420429* 0.569775* 0.505924* 0.232174* 0.480130* 0.534824* 0.480403* 0.464707* 0.459523*
Cytoplasmic Foci 0.226147* 0.247795* 0.011003 0.052150 0.166128 0.161695 0.024715 0.030273 0.199344* 0.186430* 0.011783 0.037824
Eisosomes 0.000145 0.000150 0.000029 0.000099 0.000113 0.000108 0.000100 0.000071 0.000131 0.000120 0.000033 0.000081
Endoplasmic Reticulum 0.000688 0.000827 0.000971 0.003200 0.000967 0.000576 0.004027 0.001226 0.000813 0.000648 0.001145 0.001908
Endosome 0.008264 0.029100 0.001276 0.009039 0.004856 0.013885 0.006937 0.008581 0.006742 0.018256 0.001598 0.008739
Golgi 0.001492 0.004456 0.000132 0.004069 0.001457 0.002183 0.000092 0.004238 0.001476 0.002836 0.000130 0.004180
Lipid Particles 0.008476 0.012329 0.000696 0.002924 0.005609 0.006198 0.003581 0.001636 0.007196 0.007959 0.000860 0.002081
Mitochondria 0.003233 0.011464 0.000820 0.003736 0.001703 0.007452 0.000837 0.004077 0.002550 0.008604 0.000821 0.003959
Mitotic Spindle 0.001637 0.004635 0.006115 0.012564 0.000279 0.007486 0.000904 0.032908 0.001031 0.006667 0.005819 0.025886
None 0.002616 0.003294 0.003797 0.005735 0.004526 0.003202 0.003660 0.006544 0.003469 0.003228 0.003789 0.006265
Nuclear Periphery 0.000451 0.000655 0.001509 0.001193 0.000333 0.000589 0.001719 0.001103 0.000399 0.000608 0.001521 0.001134
Nuclear Periphery Foci 0.000311 0.000552 0.000264 0.001621 0.000239 0.000261 0.001329 0.000624 0.000279 0.000344 0.000324 0.000968
Nucleolus 0.001012 0.003251 0.000317 0.000514 0.000740 0.001395 0.000969 0.001086 0.000891 0.001928 0.000354 0.000889
Nucleus 0.174758* 0.127227 0.442242* 0.285177* 0.206955* 0.165438* 0.632246* 0.288320* 0.189137* 0.154460* 0.453049* 0.287235*
Peroxisomes 0.005297 0.012833 0.000681 0.001425 0.002698 0.006436 0.000708 0.002243 0.004136 0.008274 0.000683 0.001960
Vacuole 0.037551 0.083502 0.034083 0.158583 0.024963 0.078089 0.075339 0.109453 0.031930 0.079644 0.036429 0.126411
Vacuole Periphery 0.000580 0.002943 0.000321 0.002428 0.000485 0.002509 0.000499 0.002598 0.000538 0.002633 0.000331 0.002539

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.32 0.32 -6.18 -0.73 -6.67 -4.22 -4.68 -4.27 -1.85 -0.88 -4.98 -1.66 -5.78 -2.10 -5.35
Bud Neck -3.26 5.43 5.05 6.66 0.90 -9.81 1.03 0.39 9.46 -0.83 -7.30 3.99 5.43 11.48 1.47
Bud Site -4.82 -3.26 -4.03 -1.99 -2.73 -7.77 -1.27 -6.10 0.99 -2.88 -8.24 -4.39 -6.69 -0.45 -3.10
Cell Periphery -3.30 2.65 -4.63 -1.86 -5.72 -1.18 -2.21 0.36 1.27 2.55 -1.51 3.00 -2.02 1.05 -4.34
Cytoplasm 7.53 8.75 12.71 4.23 6.58 6.98 17.84 16.19 9.87 -9.43 7.70 15.69 19.07 11.02 3.80
Cytoplasmic Foci -2.31 41.87 31.19 24.30 -9.95 0.85 24.91 29.71 30.72 0.31 2.56 52.16 44.79 37.92 -11.66
Eisosomes -0.48 18.32 5.12 4.54 -14.60 0.80 -0.63 7.52 7.02 4.33 1.93 21.48 10.36 7.80 -15.53
Endoplasmic Reticulum -0.72 -4.31 -8.80 -9.15 -7.25 1.01 -5.75 -2.27 -8.87 5.15 0.81 -4.51 -7.46 -12.62 -4.85
Endosome -7.79 12.86 1.64 8.03 -4.55 -10.47 -0.96 -0.06 8.51 0.88 -11.50 13.89 1.97 11.51 -6.15
Golgi -3.75 6.24 -0.54 1.70 -1.82 -1.30 3.63 -0.60 0.40 -3.13 -3.29 6.51 -0.83 1.30 -3.48
Lipid Particles -1.68 10.50 7.13 4.53 -8.01 -0.82 2.73 9.86 9.00 4.91 -0.76 14.00 11.27 8.47 -7.11
Mitochondria -3.39 6.44 1.45 3.77 -1.73 -5.93 2.33 -0.70 4.85 -2.28 -6.21 6.56 0.79 6.15 -2.83
Mitotic Spindle -1.98 -3.55 -3.33 -1.94 -1.68 -5.12 -0.75 -7.66 -4.83 -7.36 -5.00 -4.48 -8.16 -5.09 -5.79
None -0.67 -2.36 -1.09 -0.58 -0.35 2.09 2.31 -0.55 -2.04 -2.29 0.48 -0.46 -1.69 -1.87 -1.46
Nuclear Periphery -1.69 -18.64 -10.19 -6.95 2.87 -5.23 -10.83 -15.52 -12.33 0.67 -3.89 -24.06 -17.59 -14.32 0.86
Nuclear Periphery Foci -3.05 -0.73 -8.08 -6.90 -7.95 -0.79 -5.32 -7.89 -7.31 3.37 -1.76 -3.17 -10.35 -9.72 -8.26
Nucleolus -2.32 4.28 0.45 2.36 -1.22 -3.98 -0.45 0.26 2.98 0.53 -3.37 5.40 0.77 3.46 -1.93
Nucleus 6.35 -39.94 -11.15 -14.36 15.27 6.26 -16.55 -10.49 -15.85 12.56 7.15 -42.62 -16.25 -20.88 21.88
Peroxisomes -5.60 7.23 6.82 9.60 -1.04 -4.42 4.44 3.05 7.00 -1.59 -5.68 7.52 7.18 11.15 -1.49
Vacuole -9.98 -5.26 -20.58 -14.46 -18.96 -16.88 -6.72 -25.97 -17.13 -5.98 -18.43 -9.40 -32.60 -22.45 -27.03
Vacuole Periphery -3.71 3.51 -1.44 1.96 -2.04 -4.54 0.46 -1.76 2.09 -1.82 -5.71 3.21 -2.20 2.79 -2.95
Standard name
Human Ortholog
Description Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lpx1

Lpx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lpx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available