Standard name | |
---|---|
Description | Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 77 | 49 | 50 | 27 | 84 | 71 | 80 | 52 | 32 | 65 | 27 | 43 | – | 31 | 48 | 45 | 46 | 31 | 25 | 35 | 15 | 3 | 8 | 8 |
Bud | 31 | 27 | 32 | 19 | 55 | 52 | 69 | 65 | 45 | 60 | 44 | 56 | – | 12 | 12 | 31 | 29 | 17 | 17 | 13 | 8 | 0 | 5 | 6 |
Bud Neck | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 7 | 0 | 53 | 65 | 26 | 9 | 9 | 16 |
Bud Site | 18 | 11 | 9 | 6 | 17 | 9 | 17 | 5 | 2 | 8 | 6 | 8 | – | 6 | 9 | 23 | 16 | 10 | – | – | – | – | – | – |
Cell Periphery | 168 | 103 | 77 | 102 | 84 | 154 | 197 | 131 | 166 | 165 | 160 | 198 | – | 52 | 82 | 33 | 42 | 49 | 162 | 78 | 81 | 29 | 38 | 48 |
Cytoplasm | 91 | 90 | 49 | 112 | 141 | 178 | 315 | 299 | 248 | 341 | 294 | 334 | – | 39 | 60 | 97 | 58 | 76 | 18 | 35 | 8 | 5 | 7 | 17 |
Endoplasmic Reticulum | 9 | 7 | 5 | 12 | 23 | 21 | 34 | 10 | 14 | 11 | 8 | 10 | – | 2 | 5 | 12 | 5 | 8 | 26 | 17 | 12 | 2 | 11 | 9 |
Endosome | 0 | 1 | 1 | 5 | 6 | 0 | 3 | 11 | 2 | 7 | 11 | 4 | – | 0 | 9 | 7 | 11 | 12 | 1 | 1 | 1 | 0 | 0 | 0 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 0 | 0 | 2 | 0 | 1 | 13 | 38 | 46 | 35 | 53 | 42 | 73 | – | 0 | 0 | 10 | 6 | 6 | 1 | 0 | 1 | 1 | 1 | 0 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 1 | 3 | 6 | 1 | 2 | 1 | 2 | – | 0 | 1 | 9 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 17 | 1 | 1 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 3 | 0 | 2 | 1 | 3 | 23 | 5 | 6 | 7 | 15 | – | 1 | 3 | 24 | 26 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 1 | 3 | 6 | – | 1 | 0 | 5 | 6 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 3 | 6 | 11 | 11 | 2 | 5 | 10 | 6 | 19 | 14 | 30 | – | 3 | 14 | 74 | 69 | 68 | 0 | 0 | 2 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 2 | 3 | 4 | 8 | 3 | 23 | 29 | 16 | 28 | 16 | 13 | – | 0 | 8 | 14 | 14 | 10 | 2 | 3 | 0 | 2 | 0 | 0 |
Unique Cell Count | 259 | 199 | 148 | 194 | 295 | 334 | 538 | 520 | 382 | 521 | 423 | 529 | 102 | 164 | 270 | 225 | 223 | 312 | 255 | 161 | 57 | 85 | 114 | |
Labelled Cell Count | 400 | 296 | 237 | 301 | 434 | 506 | 789 | 706 | 575 | 767 | 636 | 793 | 147 | 251 | 385 | 343 | 314 | 312 | 255 | 161 | 57 | 85 | 114 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 13.0 | 9.4 | 9.3 | 9.8 | 9.2 | 8.8 | 8.9 | 9.2 | 8.8 | 8.8 | 9.4 | – | 11.5 | 11.5 | 12.5 | 13.5 | 13.3 | 12.9 | 13.0 | 13.3 |
Std Deviation (1e-4) | 2.4 | 2.3 | 1.8 | 1.9 | 2.0 | 1.8 | 2.0 | 2.1 | 2.2 | 2.1 | 2.1 | 2.4 | – | 1.7 | 2.0 | 3.7 | 3.9 | 3.8 | 2.4 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.06 | -0.02 | -0.09 | -0.07 | -0.02 | -0.09 | -0.1 | 0.01 | – | 0.3 | 0.3 | 0.42 | 0.53 | 0.5 | 0.46 | 0.48 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -4.4 | -1.1 | -2.9 | -5.2 | -7.1 | -7.3 | -6.1 | -8.4 | -8.0 | – | -0.6 | -0.9 | -4.0 | -2.9 | -4.5 |
Bud | -3.0 | -0.7 | -1.6 | -2.7 | -2.8 | -2.9 | -3.2 | -3.5 | -3.5 | – | -2.0 | -3.6 | -2.8 | -2.2 | -3.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.3 | -0.1 | -1.8 | -1.6 | 0 | 0 | 0 | 0 | 0 | – | -0.1 | -0.2 | 0.9 | 0.4 | -0.7 |
Cell Periphery | 0.1 | -4.9 | -1.2 | -3.4 | -6.2 | -1.8 | -4.5 | -3.0 | -3.2 | – | -0.2 | -0.4 | -8.8 | -6.8 | -6.0 |
Cytoplasm | 4.5 | 2.9 | 4.1 | 5.5 | 5.2 | 6.6 | 7.0 | 7.8 | 6.5 | – | 0.8 | 0.6 | 0.6 | -1.5 | 0.2 |
Endoplasmic Reticulum | 1.2 | 1.8 | 1.3 | 1.4 | 0 | 0.2 | 0 | 0 | 0 | – | 0 | 0 | 0.5 | 0 | 0.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.6 | 3.1 | 3.2 | 3.4 | 3.4 | 4.3 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.7 | 3.4 | 1.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.7 | -0.2 | 0 | 0 | 0 | 0 | -0.2 | -0.4 | 0.8 | – | 0 | 1.6 | 5.8 | 6.3 | 6.2 |
Vacuole | 0 | 0 | 0 | 1.3 | 1.8 | 1.2 | 1.7 | 0 | 0 | – | 0 | 1.4 | 1.6 | 1.9 | 1.3 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|