Standard name
Description Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.25 0.34 0.14 0.28 0.21 0.15 0.1 0.08 0.12 0.06 0.08 – 0.3 0.29 0.17 0.2 0.14 0.08 0.14 0.1 0.06 0.1 0.08
Bud 0.12 0.14 0.22 0.1 0.19 0.16 0.13 0.12 0.12 0.12 0.1 0.11 – 0.12 0.07 0.11 0.13 0.08 0.06 0.05 0.05 0 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.17 0.26 0.17 0.16 0.11 0.14
Bud Site 0.07 0.06 0.06 0 0.06 0 0 0 0 0 0 0 – 0.06 0.05 0.09 0.07 0 – – – – – –
Cell Periphery 0.65 0.52 0.52 0.53 0.28 0.46 0.37 0.25 0.43 0.32 0.38 0.37 – 0.51 0.5 0.12 0.19 0.22 0.52 0.31 0.5 0.51 0.45 0.42
Cytoplasm 0.35 0.45 0.33 0.58 0.48 0.53 0.59 0.57 0.65 0.65 0.7 0.63 – 0.38 0.37 0.36 0.26 0.34 0.06 0.14 0.06 0.09 0.09 0.15
Endoplasmic Reticulum 0 0 0 0.06 0.08 0.06 0.06 0 0 0 0 0 – 0 0 0 0 0 0.08 0.07 0.08 0 0.14 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.09 0.09 0.1 0.1 0.14 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0.12 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0.06 – 0 0.09 0.27 0.31 0.3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.05 0 0 – 0 0.05 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 77 49 50 27 84 71 80 52 32 65 27 43 – 31 48 45 46 31 25 35 15 3 8 8
Bud 31 27 32 19 55 52 69 65 45 60 44 56 – 12 12 31 29 17 17 13 8 0 5 6
Bud Neck 1 0 0 1 1 0 0 0 0 0 0 0 – 0 0 1 7 0 53 65 26 9 9 16
Bud Site 18 11 9 6 17 9 17 5 2 8 6 8 – 6 9 23 16 10 – – – – – –
Cell Periphery 168 103 77 102 84 154 197 131 166 165 160 198 – 52 82 33 42 49 162 78 81 29 38 48
Cytoplasm 91 90 49 112 141 178 315 299 248 341 294 334 – 39 60 97 58 76 18 35 8 5 7 17
Endoplasmic Reticulum 9 7 5 12 23 21 34 10 14 11 8 10 – 2 5 12 5 8 26 17 12 2 11 9
Endosome 0 1 1 5 6 0 3 11 2 7 11 4 – 0 9 7 11 12 1 1 1 0 0 0
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 3 0 1 1 0 1 0 0
Mitochondria 0 0 2 0 1 13 38 46 35 53 42 73 – 0 0 10 6 6 1 0 1 1 1 0
Nucleus 0 2 0 0 1 1 3 6 1 2 1 2 – 0 1 9 5 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 17 1 1 3 1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 3 0 2 1 3 23 5 6 7 15 – 1 3 24 26 12 0 0 0 0 0 0
Peroxisomes 0 1 0 2 0 1 0 2 2 1 3 6 – 1 0 5 6 12 0 0 0 0 0 0
SpindlePole 2 3 6 11 11 2 5 10 6 19 14 30 – 3 14 74 69 68 0 0 2 0 0 2
Vac/Vac Membrane 0 2 3 4 8 3 23 29 16 28 16 13 – 0 8 14 14 10 2 3 0 2 0 0
Unique Cell Count 259 199 148 194 295 334 538 520 382 521 423 529 102 164 270 225 223 312 255 161 57 85 114
Labelled Cell Count 400 296 237 301 434 506 789 706 575 767 636 793 147 251 385 343 314 312 255 161 57 85 114


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 13.0 9.4 9.3 9.8 9.2 8.8 8.9 9.2 8.8 8.8 9.4 – 11.5 11.5 12.5 13.5 13.3 12.9 13.0 13.3
Std Deviation (1e-4) 2.4 2.3 1.8 1.9 2.0 1.8 2.0 2.1 2.2 2.1 2.1 2.4 – 1.7 2.0 3.7 3.9 3.8 2.4 2.4 2.4
Intensity Change (Log2) – – – -0.02 0.06 -0.02 -0.09 -0.07 -0.02 -0.09 -0.1 0.01 – 0.3 0.3 0.42 0.53 0.5 0.46 0.48 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.4 -1.1 -2.9 -5.2 -7.1 -7.3 -6.1 -8.4 -8.0 – -0.6 -0.9 -4.0 -2.9 -4.5
Bud -3.0 -0.7 -1.6 -2.7 -2.8 -2.9 -3.2 -3.5 -3.5 – -2.0 -3.6 -2.8 -2.2 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site -1.3 -0.1 -1.8 -1.6 0 0 0 0 0 – -0.1 -0.2 0.9 0.4 -0.7
Cell Periphery 0.1 -4.9 -1.2 -3.4 -6.2 -1.8 -4.5 -3.0 -3.2 – -0.2 -0.4 -8.8 -6.8 -6.0
Cytoplasm 4.5 2.9 4.1 5.5 5.2 6.6 7.0 7.8 6.5 – 0.8 0.6 0.6 -1.5 0.2
Endoplasmic Reticulum 1.2 1.8 1.3 1.4 0 0.2 0 0 0 – 0 0 0.5 0 0.1
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2.4
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 2.6 3.1 3.2 3.4 3.4 4.3 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 1.3 0 0 0 0 – 0 0 2.7 3.4 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0.7 -0.2 0 0 0 0 -0.2 -0.4 0.8 – 0 1.6 5.8 6.3 6.2
Vacuole 0 0 0 1.3 1.8 1.2 1.7 0 0 – 0 1.4 1.6 1.9 1.3