Standard name | |
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Human Ortholog | |
Description | Core component of TRAPP complexes I, II and IV; transport protein particle (TRAPP) complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII, and IV); proposed subunit of a novel complex, TRAPPIV, that may function redundantly with TRAPPIII as a GEF that activates Ypt1 during autophagy |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 5 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 7 | 7 | 1 | 13 | 5 | 3 | 0 | 8 | 8 | 6 | 9 | 8 |
Bud | – | 2 | 6 | 4 | 5 | 7 | 20 | 26 | 11 | 16 | 26 | 22 | 2 | 0 | 0 | 5 | 5 | 1 | 0 | 5 | 2 | 1 | 3 | 5 |
Bud Neck | – | 0 | 0 | 0 | 1 | 3 | 3 | 1 | 2 | 2 | 2 | 1 | 3 | 4 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 3 | 3 |
Bud Site | – | 0 | 0 | 0 | 0 | 2 | 5 | 2 | 5 | 2 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 3 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 3 | 4 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 |
Cytoplasm | – | 92 | 99 | 143 | 226 | 165 | 197 | 193 | 152 | 164 | 176 | 253 | 172 | 222 | 333 | 36 | 38 | 46 | 3 | 64 | 58 | 66 | 122 | 151 |
Endoplasmic Reticulum | – | 8 | 3 | 1 | 1 | 5 | 0 | 2 | 5 | 2 | 1 | 5 | 12 | 7 | 4 | 8 | 8 | 5 | 0 | 1 | 1 | 4 | 3 | 5 |
Endosome | – | 13 | 2 | 5 | 18 | 3 | 3 | 8 | 4 | 22 | 5 | 3 | 8 | 8 | 26 | 45 | 43 | 38 | 2 | 24 | 13 | 18 | 14 | 11 |
Golgi | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 0 | 9 | 2 | 2 | 3 | 2 |
Mitochondria | – | 11 | 39 | 42 | 106 | 145 | 249 | 232 | 243 | 198 | 279 | 390 | 1 | 1 | 2 | 31 | 18 | 8 | 7 | 41 | 45 | 73 | 86 | 67 |
Nucleus | – | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 3 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 1 | 0 | 3 | 4 | 1 | 2 | 3 | 2 | 17 | 1 | 6 | 1 | 0 | 4 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 3 | 1 |
SpindlePole | – | 0 | 2 | 5 | 8 | 6 | 22 | 18 | 13 | 27 | 16 | 25 | 5 | 6 | 3 | 29 | 25 | 29 | 0 | 2 | 3 | 7 | 9 | 9 |
Vac/Vac Membrane | – | 4 | 3 | 1 | 4 | 3 | 1 | 5 | 3 | 8 | 4 | 2 | 2 | 1 | 4 | 7 | 6 | 4 | 0 | 7 | 4 | 7 | 23 | 22 |
Unique Cell Count | 129 | 132 | 173 | 328 | 276 | 401 | 405 | 343 | 364 | 412 | 564 | 202 | 251 | 371 | 130 | 127 | 127 | 16 | 172 | 147 | 198 | 289 | 298 | |
Labelled Cell Count | 141 | 155 | 204 | 375 | 342 | 504 | 496 | 443 | 459 | 520 | 721 | 217 | 259 | 382 | 175 | 156 | 142 | 16 | 172 | 147 | 198 | 289 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.1 | 3.5 | 3.6 | 3.5 | 3.5 | 2.7 | 3.0 | 2.9 | 2.9 | 3.0 | 2.8 | 5.5 | 5.2 | 5.2 | 5.5 | 6.2 | 6.1 | 5.6 | 5.7 | 5.6 |
Std Deviation (1e-4) | – | 1.2 | 0.7 | 1.2 | 1.3 | 1.1 | 0.7 | 0.8 | 0.7 | 1.0 | 0.9 | 0.9 | 1.0 | 1.0 | 0.9 | 0.8 | 1.1 | 1.2 | 1.0 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.0 | -0.01 | -0.39 | -0.23 | -0.27 | -0.27 | -0.22 | -0.32 | 0.64 | 0.56 | 0.57 | 0.65 | 0.81 | 0.8 | 0.67 | 0.68 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | -0.2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | -1.3 | -3.0 | -5.2 | -5.5 | -6.0 | -5.9 | -6.5 | -6.2 | 2.3 | 3.4 | 4.2 | -7.7 | -7.3 | -6.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 1.6 | 1.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 7.0 | 6.9 | 6.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -8.0 | -8.9 | -10.5 | -1.0 | -3.0 | -4.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.2 | 4.8 | 5.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7055 | 0.458 | 0.7705 | 0.4778 | 0.6873 | 0.6338 | 0.6157 | 1.1624 | 0.9854 | 0.0676 | 1.1318 | 1.1905 | -1.9207 | -0.7856 | -1.3566 | -1.7378 | -1.3548 | -1.4632 |
Actin | 0.0232 | 0.0063 | 0.0105 | 0.023 | 0.0496 | 0.0187 | 0.0212 | 0.0041 | 0.0085 | 0.0059 | 0.0347 | 0.0091 | 0.0047 | 0.0029 | 0.0067 | 0.0008 | 0.0007 | 0.0099 |
Bud | 0.002 | 0.0018 | 0.002 | 0.0101 | 0.0042 | 0.0078 | 0.0005 | 0.0026 | 0.0015 | 0.0026 | 0.0008 | 0.0007 | 0.0001 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.0035 | 0.0005 | 0.0058 | 0.0024 | 0.0012 | 0.0013 | 0.0026 | 0.0006 | 0.0009 | 0.0007 | 0.0017 | 0.0041 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0012 |
Bud Periphery | 0.0017 | 0.0032 | 0.0062 | 0.0121 | 0.009 | 0.004 | 0.0016 | 0.006 | 0.0039 | 0.0041 | 0.0013 | 0.0019 | 0.0002 | 0.0003 | 0.0006 | 0.0011 | 0.0001 | 0.0001 |
Bud Site | 0.0088 | 0.0113 | 0.0124 | 0.0101 | 0.0024 | 0.0046 | 0.011 | 0.0068 | 0.0081 | 0.0023 | 0.0027 | 0.0007 | 0.0013 | 0.0053 | 0.0017 | 0.0003 | 0.0001 | 0.0002 |
Cell Periphery | 0.002 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0016 | 0.0006 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0614 | 0.0935 | 0.0759 | 0.0842 | 0.0918 | 0.0983 | 0.0692 | 0.1065 | 0.0764 | 0.0504 | 0.0546 | 0.0705 | 0.0946 | 0.2198 | 0.1477 | 0.113 | 0.1982 | 0.185 |
Cytoplasmic Foci | 0.1505 | 0.1862 | 0.1639 | 0.1039 | 0.0848 | 0.202 | 0.2013 | 0.1826 | 0.1981 | 0.0953 | 0.2025 | 0.2273 | 0.2354 | 0.2017 | 0.1821 | 0.2047 | 0.227 | 0.1978 |
Eisosomes | 0.0007 | 0.0003 | 0.0004 | 0.0006 | 0.0003 | 0.0002 | 0.0013 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0057 | 0.0026 | 0.0033 | 0.0033 | 0.0057 | 0.0023 | 0.0049 | 0.0021 | 0.0037 | 0.0011 | 0.003 | 0.0019 | 0.006 | 0.0037 | 0.0048 | 0.0055 | 0.0036 | 0.0028 |
Endosome | 0.1287 | 0.1222 | 0.123 | 0.096 | 0.1823 | 0.106 | 0.106 | 0.1046 | 0.1363 | 0.1763 | 0.106 | 0.1011 | 0.1719 | 0.1415 | 0.1753 | 0.2775 | 0.2429 | 0.1189 |
Golgi | 0.0281 | 0.0156 | 0.0254 | 0.0306 | 0.049 | 0.0192 | 0.0214 | 0.0179 | 0.0255 | 0.0418 | 0.0396 | 0.0214 | 0.0273 | 0.0354 | 0.0282 | 0.0307 | 0.0671 | 0.0313 |
Lipid Particles | 0.04 | 0.0142 | 0.0287 | 0.0127 | 0.0181 | 0.0096 | 0.0301 | 0.0185 | 0.0262 | 0.0108 | 0.0175 | 0.0214 | 0.0329 | 0.014 | 0.02 | 0.0199 | 0.0277 | 0.0095 |
Mitochondria | 0.0178 | 0.0091 | 0.0227 | 0.0316 | 0.0164 | 0.0068 | 0.0075 | 0.0055 | 0.0117 | 0.0069 | 0.0055 | 0.0129 | 0.0075 | 0.0123 | 0.0069 | 0.015 | 0.0045 | 0.0073 |
None | 0.451 | 0.4867 | 0.4554 | 0.4804 | 0.4248 | 0.476 | 0.4586 | 0.5125 | 0.4384 | 0.5764 | 0.4049 | 0.4669 | 0.3654 | 0.324 | 0.3751 | 0.2425 | 0.1706 | 0.3983 |
Nuclear Periphery | 0.0088 | 0.0012 | 0.0018 | 0.005 | 0.0063 | 0.0046 | 0.0035 | 0.0006 | 0.0018 | 0.0006 | 0.0037 | 0.0008 | 0.0047 | 0.0008 | 0.0015 | 0.0023 | 0.0031 | 0.0006 |
Nucleolus | 0.0014 | 0.0008 | 0.0015 | 0.0024 | 0.0004 | 0.0005 | 0.001 | 0.0005 | 0.0011 | 0.0002 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0002 |
Nucleus | 0.0047 | 0.0014 | 0.0014 | 0.0278 | 0.0019 | 0.0014 | 0.0018 | 0.0011 | 0.0012 | 0.0004 | 0.001 | 0.0009 | 0.0016 | 0.0008 | 0.0012 | 0.001 | 0.0011 | 0.0007 |
Peroxisomes | 0.046 | 0.024 | 0.0496 | 0.0388 | 0.0382 | 0.0279 | 0.0453 | 0.0194 | 0.0469 | 0.0178 | 0.11 | 0.0512 | 0.0339 | 0.028 | 0.0325 | 0.0717 | 0.041 | 0.0315 |
Punctate Nuclear | 0.0074 | 0.0067 | 0.003 | 0.0093 | 0.0047 | 0.0049 | 0.0055 | 0.0012 | 0.0034 | 0.0012 | 0.0051 | 0.0035 | 0.0048 | 0.0011 | 0.0063 | 0.0022 | 0.0037 | 0.0022 |
Vacuole | 0.0045 | 0.0103 | 0.0052 | 0.0104 | 0.0062 | 0.0026 | 0.0035 | 0.0049 | 0.0042 | 0.0033 | 0.0034 | 0.002 | 0.0046 | 0.0053 | 0.0058 | 0.0078 | 0.0062 | 0.0016 |
Vacuole Periphery | 0.0018 | 0.002 | 0.0015 | 0.0046 | 0.0025 | 0.001 | 0.0008 | 0.001 | 0.0013 | 0.0016 | 0.001 | 0.0007 | 0.0014 | 0.0012 | 0.0013 | 0.0028 | 0.0017 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.815 | 18.3341 | 23.5815 | 22.6024 | 32.7959 | 35.5299 | 19.4645 | 23.7721 | 23.7292 | 31.4757 |
Translational Efficiency | 1.1323 | 1.3363 | 0.8673 | 0.9631 | 1.0092 | 1.1004 | 1.3017 | 1.1226 | 0.9793 | 1.2185 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1802 | 1702 | 338 | 931 | 1679 | 1766 | 1847 | 1776 | 3481 | 3468 | 2185 | 2707 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 653.15 | 742.23 | 1008.81 | 803.75 | 677.41 | 702.42 | 860.41 | 862.53 | 664.85 | 721.96 | 883.37 | 842.31 |
Standard Deviation | 72.47 | 102.11 | 118.14 | 118.13 | 77.01 | 95.02 | 108.69 | 114.74 | 75.67 | 100.55 | 122.58 | 119.23 |
Intensity Change Log 2 | — | 0.184452 | 0.627168 | 0.299332 | — | 0.052305 | 0.344995 | 0.348545 | — | 0.118686 | 0.490403 | 0.324597 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000359 | 0.000488 | 0.007489 | 0.001812 | 0.000513 | 0.000633 | 0.005102 | 0.001740 | 0.000433 | 0.000562 | 0.005472 | 0.001765 |
Bud Neck | 0.023279 | 0.026009 | 0.009319 | 0.015004 | 0.018812 | 0.035770 | 0.017003 | 0.013298 | 0.021124 | 0.030980 | 0.015814 | 0.013885 |
Bud Site | 0.006290 | 0.008977 | 0.025438 | 0.031482 | 0.004970 | 0.013761 | 0.019021 | 0.040347 | 0.005654 | 0.011413 | 0.020013 | 0.037298 |
Cell Periphery | 0.000120 | 0.000164 | 0.000217 | 0.000660 | 0.000167 | 0.000174 | 0.000095 | 0.000552 | 0.000143 | 0.000169 | 0.000114 | 0.000589 |
Cytoplasm | 0.369138* | 0.325319* | 0.110184* | 0.400170* | 0.341880* | 0.283197* | 0.183994* | 0.499181* | 0.355991* | 0.303869* | 0.172577* | 0.465129* |
Cytoplasmic Foci | 0.359426* | 0.349030* | 0.241331* | 0.097281 | 0.392603* | 0.373026* | 0.267429* | 0.071179 | 0.375428* | 0.361249* | 0.263392* | 0.080156 |
Eisosomes | 0.000261 | 0.000204 | 0.000121 | 0.000084 | 0.000377 | 0.000265 | 0.000083 | 0.000064 | 0.000317 | 0.000235 | 0.000089 | 0.000071 |
Endoplasmic Reticulum | 0.000994 | 0.000846 | 0.006868 | 0.001429 | 0.001077 | 0.000527 | 0.001440 | 0.001009 | 0.001034 | 0.000683 | 0.002279 | 0.001154 |
Endosome | 0.026203 | 0.040983 | 0.130466 | 0.037363 | 0.028610 | 0.054942 | 0.096283 | 0.019729 | 0.027364 | 0.048091 | 0.101571 | 0.025794 |
Golgi | 0.014283 | 0.024524 | 0.030269 | 0.011803 | 0.021398 | 0.032799 | 0.023164 | 0.004782 | 0.017715 | 0.028738 | 0.024263 | 0.007196 |
Lipid Particles | 0.020276 | 0.014609 | 0.030806 | 0.006242 | 0.023927 | 0.016745 | 0.020291 | 0.002077 | 0.022037 | 0.015697 | 0.021917 | 0.003509 |
Mitochondria | 0.011379 | 0.016684 | 0.000580 | 0.007513 | 0.015708 | 0.029949 | 0.005223 | 0.003734 | 0.013467 | 0.023439 | 0.004505 | 0.005033 |
Mitotic Spindle | 0.001380 | 0.008488 | 0.119608* | 0.040281 | 0.004425 | 0.007776 | 0.126828* | 0.032694 | 0.002849 | 0.008126 | 0.125712* | 0.035303 |
None | 0.001947 | 0.004793 | 0.000696 | 0.009179 | 0.002104 | 0.001854 | 0.001499 | 0.003749 | 0.002023 | 0.003297 | 0.001375 | 0.005617 |
Nuclear Periphery | 0.000402 | 0.000512 | 0.002491 | 0.001418 | 0.000317 | 0.000244 | 0.001277 | 0.001054 | 0.000361 | 0.000375 | 0.001464 | 0.001179 |
Nuclear Periphery Foci | 0.000290 | 0.000593 | 0.021229 | 0.003799 | 0.000528 | 0.000400 | 0.004399 | 0.000724 | 0.000405 | 0.000495 | 0.007003 | 0.001782 |
Nucleolus | 0.001137 | 0.002059 | 0.001665 | 0.001706 | 0.000988 | 0.001754 | 0.002835 | 0.000668 | 0.001065 | 0.001904 | 0.002654 | 0.001025 |
Nucleus | 0.097807 | 0.082379 | 0.065327 | 0.129427 | 0.076249 | 0.049644 | 0.064319 | 0.143522* | 0.087409 | 0.065709 | 0.064475 | 0.138674* |
Peroxisomes | 0.018107 | 0.029459 | 0.042943 | 0.017595 | 0.024515 | 0.034144 | 0.085663 | 0.015480 | 0.021198 | 0.031845 | 0.079055 | 0.016208 |
Vacuole | 0.045341 | 0.060482 | 0.150846 | 0.177233 | 0.039181 | 0.058270 | 0.073143 | 0.141671 | 0.042370 | 0.059356 | 0.085163 | 0.153901 |
Vacuole Periphery | 0.001582 | 0.003400 | 0.002104 | 0.008519 | 0.001649 | 0.004124 | 0.000906 | 0.002746 | 0.001614 | 0.003768 | 0.001092 | 0.004731 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.73 | -4.69 | -2.80 | -2.19 | 4.20 | -0.93 | -8.82 | -1.12 | -0.85 | 5.48 | -1.73 | -10.16 | -1.86 | -1.43 | 7.27 |
Bud Neck | -1.21 | 5.92 | 5.83 | 7.14 | -0.82 | -7.24 | 0.04 | 4.80 | 11.00 | 4.97 | -6.09 | 2.78 | 7.78 | 13.20 | 4.66 |
Bud Site | -2.69 | -6.61 | -4.57 | -3.61 | 1.71 | -7.15 | -9.47 | -9.33 | -4.79 | -2.99 | -7.15 | -10.58 | -10.08 | -6.50 | -1.99 |
Cell Periphery | -2.16 | -5.73 | -1.15 | -0.89 | -0.51 | -0.21 | 3.96 | -0.81 | -0.78 | -1.20 | -1.75 | 2.55 | -1.29 | -1.10 | -1.49 |
Cytoplasm | 4.73 | 20.49 | 2.46 | -1.34 | -15.56 | 6.48 | 16.19 | -9.87 | -16.16 | -25.62 | 8.04 | 23.91 | -6.50 | -13.54 | -27.51 |
Cytoplasmic Foci | 1.22 | 11.93 | 43.93 | 41.62 | 14.25 | 2.32 | 18.41 | 51.79 | 50.73 | 34.32* | 2.37 | 21.36 | 68.29 | 65.84 | 37.39* |
Eisosomes | 3.27 | 8.94 | 13.78 | 9.13 | 5.43 | 4.30 | 12.81 | 13.82 | 14.86 | 5.64 | 5.28 | 17.28 | 19.21 | 17.38 | 6.41 |
Endoplasmic Reticulum | 0.88 | -9.72 | -6.46 | -8.63 | 7.97 | 2.80 | -3.70 | -1.04 | -7.66 | 4.20 | 2.76 | -9.31 | -4.20 | -10.41 | 7.13 |
Endosome | -6.25 | -11.60 | 2.49 | 7.06 | 12.10 | -9.28 | -15.22 | 8.29 | 15.95 | 20.32* | -11.19 | -19.21 | 8.24 | 17.39 | 22.81 |
Golgi | -4.55 | -2.50 | 5.27 | 8.59 | 4.76 | -4.19 | 1.56 | 11.32 | 13.68 | 11.85 | -6.24 | -0.99 | 12.63 | 16.55 | 12.02 |
Lipid Particles | 3.41 | -2.69 | 10.42 | 8.91 | 8.19 | 4.22 | 2.36 | 16.83 | 13.15 | 14.27 | 5.31 | 0.61 | 19.66 | 16.74 | 15.72 |
Mitochondria | -2.79 | 9.01 | 5.23 | 7.85 | -2.93 | -5.61 | 6.35 | 8.16 | 14.16 | 4.90 | -6.30 | 8.53 | 10.10 | 16.43 | 3.18 |
Mitotic Spindle | -4.49 | -8.74 | -6.79 | -4.63 | 6.25 | -2.12 | -21.07* | -7.25 | -5.99 | 16.15* | -4.77 | -23.31 | -10.35 | -7.46 | 17.25 |
None | -3.40 | 3.55 | -2.78 | -0.65 | -3.64 | 0.51 | 0.94 | -1.94 | -2.53 | -3.17 | -2.68 | 1.62 | -3.50 | -1.16 | -4.42 |
Nuclear Periphery | -1.23 | -4.68 | -12.40 | -10.97 | 0.46 | 1.98 | -12.83 | -16.05 | -17.44 | 0.68 | -0.34 | -12.73 | -19.66 | -18.43 | -0.15 |
Nuclear Periphery Foci | -1.62 | -7.74 | -3.83 | -3.20 | 6.49 | 0.12 | -7.38 | -1.68 | -1.58 | 7.03 | -1.10 | -10.55 | -4.41 | -3.45 | 8.44 |
Nucleolus | -3.82 | -1.79 | -1.43 | 2.06 | 0.93 | -1.96 | -7.28 | 0.08 | 2.03 | 7.52 | -3.52 | -7.76 | -0.52 | 3.11 | 7.31 |
Nucleus | 3.41 | 2.17 | -10.67 | -12.77 | -8.72 | 7.80 | 0.36 | -19.28 | -24.99 | -17.92 | 7.65 | 3.18 | -21.00 | -26.29 | -20.50 |
Peroxisomes | -5.02 | -3.70 | 4.17 | 7.72 | 5.24 | -4.01 | -13.30 | 7.43 | 11.30 | 18.03* | -6.46 | -15.11 | 8.38 | 13.56 | 18.83 |
Vacuole | -5.54 | -11.54 | -26.92 | -24.94 | -8.28 | -6.61 | -15.21 | -30.40 | -26.00 | -18.47 | -8.53 | -18.40 | -40.08 | -35.75 | -22.76 |
Vacuole Periphery | -4.85 | -1.11 | -1.40 | -0.10 | -1.15 | -6.64 | 5.72 | 0.57 | 4.82 | -1.28 | -8.17 | 5.85 | -0.89 | 2.69 | -2.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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